Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038492.t1 | XP_003628734.2 | 98.5 | 197 | 3 | 0 | 1 | 197 | 98 | 294 | 1.80E-103 | 385.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038492.t1 | Q4R599 | 34.6 | 185 | 101 | 4 | 8 | 175 | 85 | 266 | 8.0e-23 | 108.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038492.t1 | G7LG96 | 98.5 | 197 | 3 | 0 | 1 | 197 | 98 | 294 | 1.3e-103 | 385.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene038492 | MS.gene014694 | PPI |
| MS.gene038492 | MS.gene055453 | PPI |
| MS.gene073052 | MS.gene038492 | PPI |
| MS.gene038492 | MS.gene22417 | PPI |
| MS.gene055455 | MS.gene038492 | PPI |
| MS.gene038492 | MS.gene40349 | PPI |
| MS.gene038492 | MS.gene007531 | PPI |
| MS.gene055454 | MS.gene038492 | PPI |
| MS.gene007416 | MS.gene038492 | PPI |
| MS.gene20079 | MS.gene038492 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038492.t1 | MTR_8g066180 | 98.477 | 197 | 3 | 0 | 1 | 197 | 98 | 294 | 4.13e-145 | 405 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038492.t1 | AT2G03780 | 77.551 | 196 | 44 | 0 | 1 | 196 | 49 | 244 | 3.71e-113 | 322 |
| MS.gene038492.t1 | AT2G03780 | 77.551 | 196 | 44 | 0 | 1 | 196 | 7 | 202 | 3.96e-113 | 321 |
| MS.gene038492.t1 | AT2G03780 | 77.551 | 196 | 44 | 0 | 1 | 196 | 92 | 287 | 5.91e-113 | 323 |
Find 36 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATCCTTGACTATCTATT+AGG | 0.234815 | 8.4:-36455181 | MS.gene038492:CDS |
| ACATCCTTGACTATCTATTA+GGG | 0.277012 | 8.4:-36455180 | MS.gene038492:CDS |
| TCAGAGTATATACCACTTCT+AGG | 0.333170 | 8.4:-36454203 | MS.gene038492:CDS |
| ATACCTCATAGTTCAATATC+TGG | 0.349942 | 8.4:+36454146 | None:intergenic |
| GCTTTGCACGTGATATATAT+AGG | 0.370536 | 8.4:-36454838 | MS.gene038492:CDS |
| TCGACTAGTCAAAGAATTGC+AGG | 0.386016 | 8.4:-36455451 | MS.gene038492:CDS |
| GAGCTGATGCGTTTGGCTAT+TGG | 0.405037 | 8.4:-36454905 | MS.gene038492:CDS |
| GACCGGAGAGCTGATGCGTT+TGG | 0.425505 | 8.4:-36454912 | MS.gene038492:CDS |
| TACGCTCGTTGTGCCACATA+TGG | 0.433548 | 8.4:-36454810 | MS.gene038492:CDS |
| TGATCCAAGTTCTTTCTTAG+TGG | 0.448490 | 8.4:-36454174 | MS.gene038492:CDS |
| CTTTGCACGTGATATATATA+GGG | 0.471371 | 8.4:-36454837 | MS.gene038492:CDS |
| GATCCAAGTTCTTTCTTAGT+GGG | 0.475255 | 8.4:-36454173 | MS.gene038492:CDS |
| AGTACTTGAGAAAGCAGAAA+AGG | 0.487405 | 8.4:-36455505 | MS.gene038492:CDS |
| AGCCAAACGCATCAGCTCTC+CGG | 0.501275 | 8.4:+36454910 | None:intergenic |
| TTCTGATATGAAAACAAAGA+TGG | 0.507798 | 8.4:-36454780 | MS.gene038492:CDS |
| CTAAGACGAGCCTACTCACC+CGG | 0.512679 | 8.4:-36455411 | MS.gene038492:CDS |
| TGCTTTAGTGTTCATGTGAG+AGG | 0.513044 | 8.4:-36454227 | MS.gene038492:CDS |
| ACTCCCACTAAGAAAGAACT+TGG | 0.522033 | 8.4:+36454170 | None:intergenic |
| TTTCATGACACTTTGGAGCA+TGG | 0.523941 | 8.4:+36454754 | None:intergenic |
| TTACAGCTTGCAGATTTGAC+CGG | 0.531970 | 8.4:-36454929 | MS.gene038492:intron |
| ATCTGCAGAGGCTGAAGAGA+AGG | 0.540287 | 8.4:+36455205 | None:intergenic |
| AGTGTCATGAAAATAGAGAA+TGG | 0.543198 | 8.4:-36454743 | MS.gene038492:intron |
| TGACTAGTCGAGACACGTGC+TGG | 0.545601 | 8.4:+36455463 | None:intergenic |
| AAAGTATACCCCGGGTGAGT+AGG | 0.551350 | 8.4:+36455401 | None:intergenic |
| GTTCCAGATATTGAACTATG+AGG | 0.571700 | 8.4:-36454149 | None:intergenic |
| GGATCATTTGATCCTAGAAG+TGG | 0.576528 | 8.4:+36454191 | None:intergenic |
| TCAGAACTGTCATCCATATG+TGG | 0.587378 | 8.4:+36454797 | None:intergenic |
| TGATGCGTTTGGCTATTGGT+AGG | 0.594582 | 8.4:-36454901 | MS.gene038492:CDS |
| TCAAGGATGTTTATCTGCAG+AGG | 0.603539 | 8.4:+36455193 | None:intergenic |
| GCTATTGGTAGGATATCAGA+TGG | 0.607525 | 8.4:-36454890 | MS.gene038492:CDS |
| TAAGACGAGCCTACTCACCC+GGG | 0.643500 | 8.4:-36455410 | MS.gene038492:CDS |
| CGACTAGTCAAAGAATTGCA+GGG | 0.652001 | 8.4:-36455450 | MS.gene038492:CDS |
| CATCCTTGACTATCTATTAG+GGG | 0.657819 | 8.4:-36455179 | MS.gene038492:intron |
| ATACCCCTAATAGATAGTCA+AGG | 0.666089 | 8.4:+36455176 | None:intergenic |
| AAGACGAGCCTACTCACCCG+GGG | 0.730892 | 8.4:-36455409 | MS.gene038492:intron |
| TGAAGAGAAGGATCACTAAG+CGG | 0.736059 | 8.4:+36455217 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAGATAATAAATAGTCAA+AGG | + | chr8.4:36455216-36455235 | None:intergenic | 15.0% |
| !! | TAATATTGAGAACATTTAAA+AGG | + | chr8.4:36454733-36454752 | None:intergenic | 15.0% |
| !!! | ATTTCTATTAATTTTGCAAA+AGG | + | chr8.4:36455270-36455289 | None:intergenic | 15.0% |
| !!! | TAGATATAACATTTTAACAA+GGG | - | chr8.4:36454579-36454598 | MS.gene038492:intron | 15.0% |
| !! | AATGTTGATATTAATGAGTT+TGG | - | chr8.4:36455287-36455306 | MS.gene038492:CDS | 20.0% |
| !! | TTAGAAGTAACAAAATTTAC+TGG | + | chr8.4:36455167-36455186 | None:intergenic | 20.0% |
| !!! | CTTCTTATTTGTTGTTTTAA+GGG | - | chr8.4:36455118-36455137 | MS.gene038492:intron | 20.0% |
| !!! | GTAAATTTTGTTACTTCTAA+TGG | - | chr8.4:36455167-36455186 | MS.gene038492:intron | 20.0% |
| !!! | GTAGATATAACATTTTAACA+AGG | - | chr8.4:36454578-36454597 | MS.gene038492:intron | 20.0% |
| ! | AACGGTAAGATAACAATAAA+AGG | + | chr8.4:36454539-36454558 | None:intergenic | 25.0% |
| ! | ACGGTAAGATAACAATAAAA+GGG | + | chr8.4:36454538-36454557 | None:intergenic | 25.0% |
| ! | ATACCTCAATTAAGATATTG+AGG | + | chr8.4:36455023-36455042 | None:intergenic | 25.0% |
| ! | ATCTTAATTGAGGTATGAAT+AGG | - | chr8.4:36455027-36455046 | MS.gene038492:intron | 25.0% |
| ! | CAACATCTATTATCAAGATT+TGG | + | chr8.4:36455421-36455440 | None:intergenic | 25.0% |
| ! | CCTTAAAACAACAAATAAGA+AGG | + | chr8.4:36455120-36455139 | None:intergenic | 25.0% |
| ! | CTTAATTTAGAGATTACCAA+AGG | + | chr8.4:36454675-36454694 | None:intergenic | 25.0% |
| ! | GATTACCAAAGGAAATATAA+AGG | + | chr8.4:36454664-36454683 | None:intergenic | 25.0% |
| ! | TTCTGATATGAAAACAAAGA+TGG | - | chr8.4:36454899-36454918 | MS.gene038492:CDS | 25.0% |
| ! | TTGAGAACATTTAAAAGGAA+AGG | + | chr8.4:36454728-36454747 | None:intergenic | 25.0% |
| ! | TTGTATAGAAAAACTCAATG+TGG | - | chr8.4:36455312-36455331 | MS.gene038492:intron | 25.0% |
| !! | AATAGAGAATGGTAATGTAT+TGG | - | chr8.4:36454947-36454966 | MS.gene038492:intron | 25.0% |
| !! | TGGAGTATCATTATATTCAA+TGG | + | chr8.4:36455091-36455110 | None:intergenic | 25.0% |
| !! | TTTATCTGATATACTTGTTG+TGG | - | chr8.4:36454339-36454358 | MS.gene038492:intron | 25.0% |
| !!! | ATTTTGCAAAAGGTTTTATC+AGG | + | chr8.4:36455260-36455279 | None:intergenic | 25.0% |
| !!! | CCTTCTTATTTGTTGTTTTA+AGG | - | chr8.4:36455117-36455136 | MS.gene038492:intron | 25.0% |
| !!! | TTATCTGATATACTTGTTGT+GGG | - | chr8.4:36454340-36454359 | MS.gene038492:intron | 25.0% |
| !!! | TTTTGTTACTTCTAATGGAA+TGG | - | chr8.4:36455172-36455191 | MS.gene038492:intron | 25.0% |
| AACAAATAAGAAGGAACTGA+TGG | + | chr8.4:36455111-36455130 | None:intergenic | 30.0% | |
| AACATCCTTGACTATCTATT+AGG | - | chr8.4:36454498-36454517 | MS.gene038492:intron | 30.0% | |
| ACATCCTTGACTATCTATTA+GGG | - | chr8.4:36454499-36454518 | MS.gene038492:intron | 30.0% | |
| ACTCAACAACATGAATAGAA+CGG | + | chr8.4:36454557-36454576 | None:intergenic | 30.0% | |
| AGTGTCATGAAAATAGAGAA+TGG | - | chr8.4:36454936-36454955 | MS.gene038492:intron | 30.0% | |
| CACTTATGAATCCATTGAAA+TGG | + | chr8.4:36454605-36454624 | None:intergenic | 30.0% | |
| CGGTAAGATAACAATAAAAG+GGG | + | chr8.4:36454537-36454556 | None:intergenic | 30.0% | |
| CTTTGCACGTGATATATATA+GGG | - | chr8.4:36454842-36454861 | MS.gene038492:CDS | 30.0% | |
| GATCACTTCACTTTCTATTA+GGG | + | chr8.4:36455361-36455380 | None:intergenic | 30.0% | |
| GGTAAGATAACAATAAAAGG+GGG | + | chr8.4:36454536-36454555 | None:intergenic | 30.0% | |
| TGATCACTTCACTTTCTATT+AGG | + | chr8.4:36455362-36455381 | None:intergenic | 30.0% | |
| TTCTGCAGTTTCTGTAAAAA+CGG | - | chr8.4:36454399-36454418 | MS.gene038492:intron | 30.0% | |
| ! | TCTCTATTTTCATGACACTT+TGG | + | chr8.4:36454935-36454954 | None:intergenic | 30.0% |
| !! | GTTAGCCTTTATATTTCCTT+TGG | - | chr8.4:36454656-36454675 | MS.gene038492:intron | 30.0% |
| ACGCCTCAATATCTTAATTG+AGG | - | chr8.4:36455017-36455036 | MS.gene038492:intron | 35.0% | |
| AGGATAGTCTAATGACTGAT+TGG | - | chr8.4:36455047-36455066 | MS.gene038492:intron | 35.0% | |
| AGTACTTGAGAAAGCAGAAA+AGG | - | chr8.4:36454174-36454193 | MS.gene038492:CDS | 35.0% | |
| ATACCCCTAATAGATAGTCA+AGG | + | chr8.4:36454506-36454525 | None:intergenic | 35.0% | |
| CATCCTTGACTATCTATTAG+GGG | - | chr8.4:36454500-36454519 | MS.gene038492:intron | 35.0% | |
| GATCCAAGTTCTTTCTTAGT+GGG | - | chr8.4:36455506-36455525 | MS.gene038492:CDS | 35.0% | |
| GCTTTGCACGTGATATATAT+AGG | - | chr8.4:36454841-36454860 | MS.gene038492:CDS | 35.0% | |
| TCAGAGTATATACCACTTCT+AGG | - | chr8.4:36455476-36455495 | MS.gene038492:CDS | 35.0% | |
| TGATCCAAGTTCTTTCTTAG+TGG | - | chr8.4:36455505-36455524 | MS.gene038492:CDS | 35.0% | |
| TTTAACAAGGGCCATTTCAA+TGG | - | chr8.4:36454591-36454610 | MS.gene038492:intron | 35.0% | |
| ! | AAGTAACTGAAAGTATACCC+CGG | + | chr8.4:36454290-36454309 | None:intergenic | 35.0% |
| !! | AATTGCAGGGAACTGATTTT+TGG | - | chr8.4:36454242-36454261 | MS.gene038492:intron | 35.0% |
| !! | CAAAATTTACTGGCTTGTCT+TGG | + | chr8.4:36455157-36455176 | None:intergenic | 35.0% |
| ACTCCCACTAAGAAAGAACT+TGG | + | chr8.4:36455512-36455531 | None:intergenic | 40.0% | |
| CACTTCACTTTCTATTAGGG+TGG | + | chr8.4:36455358-36455377 | None:intergenic | 40.0% | |
| CTTTCTATTAGGGTGGAAAG+AGG | + | chr8.4:36455351-36455370 | None:intergenic | 40.0% | |
| GCTATTGGTAGGATATCAGA+TGG | - | chr8.4:36454789-36454808 | MS.gene038492:CDS | 40.0% | |
| GGATCATTTGATCCTAGAAG+TGG | + | chr8.4:36455491-36455510 | None:intergenic | 40.0% | |
| TCAAGGATGTTTATCTGCAG+AGG | + | chr8.4:36454489-36454508 | None:intergenic | 40.0% | |
| TCAGAACTGTCATCCATATG+TGG | + | chr8.4:36454885-36454904 | None:intergenic | 40.0% | |
| TGAAGAGAAGGATCACTAAG+CGG | + | chr8.4:36454465-36454484 | None:intergenic | 40.0% | |
| TGCTTTAGTGTTCATGTGAG+AGG | - | chr8.4:36455452-36455471 | MS.gene038492:CDS | 40.0% | |
| TTACAGCTTGCAGATTTGAC+CGG | - | chr8.4:36454750-36454769 | MS.gene038492:CDS | 40.0% | |
| TTGTGGGTATTTGCAGATAC+AGG | - | chr8.4:36454356-36454375 | MS.gene038492:intron | 40.0% | |
| ! | AGTAACTGAAAGTATACCCC+GGG | + | chr8.4:36454289-36454308 | None:intergenic | 40.0% |
| ! | CGACTAGTCAAAGAATTGCA+GGG | - | chr8.4:36454229-36454248 | MS.gene038492:CDS | 40.0% |
| ! | TCGACTAGTCAAAGAATTGC+AGG | - | chr8.4:36454228-36454247 | MS.gene038492:CDS | 40.0% |
| !! | TTTCATGACACTTTGGAGCA+TGG | + | chr8.4:36454928-36454947 | None:intergenic | 40.0% |
| TGATGCGTTTGGCTATTGGT+AGG | - | chr8.4:36454778-36454797 | MS.gene038492:CDS | 45.0% | |
| AAAGTATACCCCGGGTGAGT+AGG | + | chr8.4:36454281-36454300 | None:intergenic | 50.0% | |
| ATCTGCAGAGGCTGAAGAGA+AGG | + | chr8.4:36454477-36454496 | None:intergenic | 50.0% | |
| GAGCTGATGCGTTTGGCTAT+TGG | - | chr8.4:36454774-36454793 | MS.gene038492:CDS | 50.0% | |
| TACGCTCGTTGTGCCACATA+TGG | - | chr8.4:36454869-36454888 | MS.gene038492:CDS | 50.0% | |
| AGCCAAACGCATCAGCTCTC+CGG | + | chr8.4:36454772-36454791 | None:intergenic | 55.0% | |
| CTAAGACGAGCCTACTCACC+CGG | - | chr8.4:36454268-36454287 | MS.gene038492:intron | 55.0% | |
| TAAGACGAGCCTACTCACCC+GGG | - | chr8.4:36454269-36454288 | MS.gene038492:intron | 55.0% | |
| ! | TGACTAGTCGAGACACGTGC+TGG | + | chr8.4:36454219-36454238 | None:intergenic | 55.0% |
| AAGACGAGCCTACTCACCCG+GGG | - | chr8.4:36454270-36454289 | MS.gene038492:intron | 60.0% | |
| GACCGGAGAGCTGATGCGTT+TGG | - | chr8.4:36454767-36454786 | MS.gene038492:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 36454151 | 36455550 | 36454151 | ID=MS.gene038492 |
| chr8.4 | mRNA | 36454151 | 36455550 | 36454151 | ID=MS.gene038492.t1;Parent=MS.gene038492 |
| chr8.4 | exon | 36455410 | 36455550 | 36455410 | ID=MS.gene038492.t1.exon1;Parent=MS.gene038492.t1 |
| chr8.4 | CDS | 36455410 | 36455550 | 36455410 | ID=cds.MS.gene038492.t1;Parent=MS.gene038492.t1 |
| chr8.4 | exon | 36455180 | 36455329 | 36455180 | ID=MS.gene038492.t1.exon2;Parent=MS.gene038492.t1 |
| chr8.4 | CDS | 36455180 | 36455329 | 36455180 | ID=cds.MS.gene038492.t1;Parent=MS.gene038492.t1 |
| chr8.4 | exon | 36454744 | 36454945 | 36454744 | ID=MS.gene038492.t1.exon3;Parent=MS.gene038492.t1 |
| chr8.4 | CDS | 36454744 | 36454945 | 36454744 | ID=cds.MS.gene038492.t1;Parent=MS.gene038492.t1 |
| chr8.4 | exon | 36454151 | 36454251 | 36454151 | ID=MS.gene038492.t1.exon4;Parent=MS.gene038492.t1 |
| chr8.4 | CDS | 36454151 | 36454251 | 36454151 | ID=cds.MS.gene038492.t1;Parent=MS.gene038492.t1 |
| Gene Sequence |
| Protein sequence |
