Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71227.t1 | XP_003628734.2 | 98.5 | 197 | 3 | 0 | 1 | 197 | 98 | 294 | 4.80E-104 | 387.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71227.t1 | Q4R599 | 34.4 | 186 | 102 | 4 | 7 | 175 | 84 | 266 | 3.6e-23 | 109.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71227.t1 | G7LG96 | 98.5 | 197 | 3 | 0 | 1 | 197 | 98 | 294 | 3.5e-104 | 387.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene055455 | MS.gene71227 | PPI |
| MS.gene71227 | MS.gene007531 | PPI |
| MS.gene71227 | MS.gene014694 | PPI |
| MS.gene055454 | MS.gene71227 | PPI |
| MS.gene007416 | MS.gene71227 | PPI |
| MS.gene20079 | MS.gene71227 | PPI |
| MS.gene71227 | MS.gene055453 | PPI |
| MS.gene22417 | MS.gene71227 | PPI |
| MS.gene073052 | MS.gene71227 | PPI |
| MS.gene40349 | MS.gene71227 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71227.t1 | MTR_8g066180 | 98.477 | 197 | 3 | 0 | 1 | 197 | 98 | 294 | 1.10e-145 | 407 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71227.t1 | AT2G03780 | 77.551 | 196 | 44 | 0 | 1 | 196 | 49 | 244 | 5.22e-114 | 325 |
| MS.gene71227.t1 | AT2G03780 | 77.551 | 196 | 44 | 0 | 1 | 196 | 7 | 202 | 5.50e-114 | 323 |
| MS.gene71227.t1 | AT2G03780 | 77.551 | 196 | 44 | 0 | 1 | 196 | 92 | 287 | 1.11e-113 | 325 |
Find 38 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATCCTTGACTATCTATT+AGG | 0.234815 | 8.3:-36694495 | MS.gene71227:CDS |
| ATCAGAGTATATACCACTTC+TGG | 0.253259 | 8.3:-36693525 | MS.gene71227:CDS |
| AAATGATCCAAGTTCTTTCT+TGG | 0.271639 | 8.3:-36693498 | MS.gene71227:CDS |
| ACATCCTTGACTATCTATTA+GGG | 0.277012 | 8.3:-36694494 | MS.gene71227:CDS |
| ATACCTCATAGTTCAATATC+TGG | 0.349942 | 8.3:+36693467 | None:intergenic |
| GCTTTGCACGTGATATATAT+AGG | 0.370536 | 8.3:-36694158 | MS.gene71227:CDS |
| TCGACTAGTCAAAGAATTGC+AGG | 0.371441 | 8.3:-36694763 | MS.gene71227:CDS |
| GAGCTAATGCGTTTGGCTAT+TGG | 0.387746 | 8.3:-36694225 | MS.gene71227:CDS |
| TACGCTTGTCGTGCCACATA+TGG | 0.407182 | 8.3:-36694130 | MS.gene71227:CDS |
| GACCGGAGAGCTAATGCGTT+TGG | 0.423648 | 8.3:-36694232 | MS.gene71227:CDS |
| CTTTGCACGTGATATATATA+GGG | 0.471371 | 8.3:-36694157 | MS.gene71227:CDS |
| AGCCAAACGCATTAGCTCTC+CGG | 0.473967 | 8.3:+36694230 | None:intergenic |
| TCAGAGTATATACCACTTCT+GGG | 0.477042 | 8.3:-36693524 | MS.gene71227:CDS |
| GATCCAAGTTCTTTCTTGGT+GGG | 0.492081 | 8.3:-36693494 | MS.gene71227:CDS |
| ACTCCCACCAAGAAAGAACT+TGG | 0.505823 | 8.3:+36693491 | None:intergenic |
| TTCTGATATGAAAACAAAGA+TGG | 0.507798 | 8.3:-36694100 | MS.gene71227:CDS |
| CTAAGACGAGCCTACTCACC+CGG | 0.512679 | 8.3:-36694723 | MS.gene71227:CDS |
| AGTACTTGAGAAAGCAGAAA+AGG | 0.512813 | 8.3:-36694817 | MS.gene71227:CDS |
| TGCTTTAGTGTTCATGTCAG+AGG | 0.516215 | 8.3:-36693548 | MS.gene71227:CDS |
| TGATCCAAGTTCTTTCTTGG+TGG | 0.519549 | 8.3:-36693495 | MS.gene71227:CDS |
| TTACAGCTTGCAGACTTGAC+CGG | 0.531897 | 8.3:-36694249 | MS.gene71227:intron |
| TGACTAGTCGAGAGACGTGC+TGG | 0.534137 | 8.3:+36694775 | None:intergenic |
| ATCTGCAGAGGCTGAAGAGA+AGG | 0.540287 | 8.3:+36694519 | None:intergenic |
| AAAGTATACCCCGGGTGAGT+AGG | 0.551350 | 8.3:+36694713 | None:intergenic |
| GTTCCAGATATTGAACTATG+AGG | 0.571700 | 8.3:-36693470 | None:intergenic |
| TTTCACGACACTTTGAAGCA+TGG | 0.577406 | 8.3:+36694074 | None:intergenic |
| GGATCATTTGATCCCAGAAG+TGG | 0.578340 | 8.3:+36693512 | None:intergenic |
| TCAGAACTGTCATCCATATG+TGG | 0.587378 | 8.3:+36694117 | None:intergenic |
| AGTGTCGTGAAAATAGAGAA+TGG | 0.588753 | 8.3:-36694063 | MS.gene71227:intron |
| TAATGCGTTTGGCTATTGGT+AGG | 0.589118 | 8.3:-36694221 | MS.gene71227:CDS |
| TCAAGGATGTTTATCTGCAG+AGG | 0.603539 | 8.3:+36694507 | None:intergenic |
| GCTATTGGTAGGATATCAGA+TGG | 0.607525 | 8.3:-36694210 | MS.gene71227:CDS |
| CGACTAGTCAAAGAATTGCA+GGG | 0.607636 | 8.3:-36694762 | MS.gene71227:CDS |
| TAAGACGAGCCTACTCACCC+GGG | 0.643500 | 8.3:-36694722 | MS.gene71227:CDS |
| CATCCTTGACTATCTATTAG+GGG | 0.657819 | 8.3:-36694493 | MS.gene71227:intron |
| ATACCCCTAATAGATAGTCA+AGG | 0.666089 | 8.3:+36694490 | None:intergenic |
| AAGACGAGCCTACTCACCCG+GGG | 0.730892 | 8.3:-36694721 | MS.gene71227:intron |
| TGAAGAGAAGGATCACTAAG+CGG | 0.736059 | 8.3:+36694531 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAGATAATAAATAGTCA+AGG | + | chr8.3:36694529-36694548 | None:intergenic | 15.0% |
| !! | ATAAGATAATAAATAGTCAA+GGG | + | chr8.3:36694528-36694547 | None:intergenic | 15.0% |
| !! | TTGTATAAAAAAACTCAATA+TGG | - | chr8.3:36694624-36694643 | MS.gene71227:CDS | 15.0% |
| !! | AATAGAGAATGGTAATATAT+CGG | - | chr8.3:36694260-36694279 | MS.gene71227:intron | 20.0% |
| !! | AATGTTGATATTAATGAGAT+TGG | - | chr8.3:36694599-36694618 | MS.gene71227:CDS | 20.0% |
| !! | AGTAGATATAACATTTAACA+AGG | - | chr8.3:36693895-36693914 | MS.gene71227:intron | 20.0% |
| !! | GTAGATATAACATTTAACAA+GGG | - | chr8.3:36693896-36693915 | MS.gene71227:intron | 20.0% |
| !!! | ATTTCTATTAATTTTGCACA+AGG | + | chr8.3:36694582-36694601 | None:intergenic | 20.0% |
| !!! | CTTCTTATTTGTTGTTTTAA+GGG | - | chr8.3:36694431-36694450 | MS.gene71227:intron | 20.0% |
| ! | AACGCTAAGATAACAATAAA+AGG | + | chr8.3:36693857-36693876 | None:intergenic | 25.0% |
| ! | ACGCTAAGATAACAATAAAA+GGG | + | chr8.3:36693856-36693875 | None:intergenic | 25.0% |
| ! | ATACCTCAATTAAGATATTG+AGG | + | chr8.3:36694336-36694355 | None:intergenic | 25.0% |
| ! | ATCTTAATTGAGGTATGTAT+AGG | - | chr8.3:36694340-36694359 | MS.gene71227:intron | 25.0% |
| ! | CAACATCTATTATCAAGATT+TGG | + | chr8.3:36694733-36694752 | None:intergenic | 25.0% |
| ! | CCTTAAAACAACAAATAAGA+AGG | + | chr8.3:36694433-36694452 | None:intergenic | 25.0% |
| ! | CTTAATAAAGAGATTACCAA+AGG | + | chr8.3:36693992-36694011 | None:intergenic | 25.0% |
| ! | TTCTGATATGAAAACAAAGA+TGG | - | chr8.3:36694212-36694231 | MS.gene71227:CDS | 25.0% |
| !! | TGGAGTATCATTATATTCAA+TGG | + | chr8.3:36694404-36694423 | None:intergenic | 25.0% |
| !! | TTTATCTGATATACTTGTTG+TGG | - | chr8.3:36693658-36693677 | MS.gene71227:intron | 25.0% |
| !!! | CCTTCTTATTTGTTGTTTTA+AGG | - | chr8.3:36694430-36694449 | MS.gene71227:intron | 25.0% |
| !!! | GTTTGCTTTTATTTTTCCTT+TGG | - | chr8.3:36693973-36693992 | MS.gene71227:intron | 25.0% |
| !!! | TTATCTGATATACTTGTTGT+GGG | - | chr8.3:36693659-36693678 | MS.gene71227:intron | 25.0% |
| AAATGATCCAAGTTCTTTCT+TGG | - | chr8.3:36694814-36694833 | MS.gene71227:CDS | 30.0% | |
| AACAAATAAGAAGGAACAGA+TGG | + | chr8.3:36694424-36694443 | None:intergenic | 30.0% | |
| AACATCCTTGACTATCTATT+AGG | - | chr8.3:36693817-36693836 | MS.gene71227:intron | 30.0% | |
| ACACCTCAATATCTTAATTG+AGG | - | chr8.3:36694330-36694349 | MS.gene71227:intron | 30.0% | |
| ACATCCTTGACTATCTATTA+GGG | - | chr8.3:36693818-36693837 | MS.gene71227:intron | 30.0% | |
| CAAGCTGTAATATTGAGAAT+AGG | + | chr8.3:36694053-36694072 | None:intergenic | 30.0% | |
| CACTTATGAATCCATTGAAA+TGG | + | chr8.3:36693922-36693941 | None:intergenic | 30.0% | |
| CGCTAAGATAACAATAAAAG+GGG | + | chr8.3:36693855-36693874 | None:intergenic | 30.0% | |
| CTTTGCACGTGATATATATA+GGG | - | chr8.3:36694155-36694174 | MS.gene71227:CDS | 30.0% | |
| GCTAAGATAACAATAAAAGG+GGG | + | chr8.3:36693854-36693873 | None:intergenic | 30.0% | |
| TCAGAAGCAACAAAATTTAC+TGG | + | chr8.3:36694479-36694498 | None:intergenic | 30.0% | |
| TTCTGCAGTTTCTGTAAAAA+CGG | - | chr8.3:36693718-36693737 | MS.gene71227:intron | 30.0% | |
| ! | ACAAAATTTACTGGTTGTCT+TGG | + | chr8.3:36694470-36694489 | None:intergenic | 30.0% |
| !! | ATTTTGCACAAGGTTTTATC+AGG | + | chr8.3:36694572-36694591 | None:intergenic | 30.0% |
| !! | GTAAATTTTGTTGCTTCTGA+TGG | - | chr8.3:36694479-36694498 | MS.gene71227:intron | 30.0% |
| AGTACTTGAGAAAGCAGAAA+AGG | - | chr8.3:36693495-36693514 | MS.gene71227:CDS | 35.0% | |
| AGTGTCGTGAAAATAGAGAA+TGG | - | chr8.3:36694249-36694268 | MS.gene71227:intron | 35.0% | |
| ATACCCCTAATAGATAGTCA+AGG | + | chr8.3:36693825-36693844 | None:intergenic | 35.0% | |
| ATCAGAGTATATACCACTTC+TGG | - | chr8.3:36694787-36694806 | MS.gene71227:CDS | 35.0% | |
| CATCCTTGACTATCTATTAG+GGG | - | chr8.3:36693819-36693838 | MS.gene71227:intron | 35.0% | |
| GCTGTAATATTGAGAATAGG+AGG | + | chr8.3:36694050-36694069 | None:intergenic | 35.0% | |
| GCTTTGCACGTGATATATAT+AGG | - | chr8.3:36694154-36694173 | MS.gene71227:CDS | 35.0% | |
| TCAGAGTATATACCACTTCT+GGG | - | chr8.3:36694788-36694807 | MS.gene71227:CDS | 35.0% | |
| TTTAACAAGGGCCATTTCAA+TGG | - | chr8.3:36693908-36693927 | MS.gene71227:intron | 35.0% | |
| ! | AAGTAACTGAAAGTATACCC+CGG | + | chr8.3:36693611-36693630 | None:intergenic | 35.0% |
| ! | GATCACGTCACTTTCTATTA+GGG | + | chr8.3:36694673-36694692 | None:intergenic | 35.0% |
| ! | TGATCACGTCACTTTCTATT+AGG | + | chr8.3:36694674-36694693 | None:intergenic | 35.0% |
| !! | AATTGCAGGGAACTGATTTT+TGG | - | chr8.3:36693563-36693582 | MS.gene71227:intron | 35.0% |
| AGGATAGTCTAGTGACTGAT+GGG | - | chr8.3:36694360-36694379 | MS.gene71227:intron | 40.0% | |
| GATCCAAGTTCTTTCTTGGT+GGG | - | chr8.3:36694818-36694837 | MS.gene71227:CDS | 40.0% | |
| GCTATTGGTAGGATATCAGA+TGG | - | chr8.3:36694102-36694121 | MS.gene71227:CDS | 40.0% | |
| TAATGCGTTTGGCTATTGGT+AGG | - | chr8.3:36694091-36694110 | MS.gene71227:CDS | 40.0% | |
| TAGGATAGTCTAGTGACTGA+TGG | - | chr8.3:36694359-36694378 | MS.gene71227:intron | 40.0% | |
| TCAAGGATGTTTATCTGCAG+AGG | + | chr8.3:36693808-36693827 | None:intergenic | 40.0% | |
| TCAGAACTGTCATCCATATG+TGG | + | chr8.3:36694198-36694217 | None:intergenic | 40.0% | |
| TGAAGAGAAGGATCACTAAG+CGG | + | chr8.3:36693784-36693803 | None:intergenic | 40.0% | |
| TGATCCAAGTTCTTTCTTGG+TGG | - | chr8.3:36694817-36694836 | MS.gene71227:CDS | 40.0% | |
| TGCTTTAGTGTTCATGTCAG+AGG | - | chr8.3:36694764-36694783 | MS.gene71227:CDS | 40.0% | |
| ! | ACGTCACTTTCTATTAGGGT+GGG | + | chr8.3:36694669-36694688 | None:intergenic | 40.0% |
| ! | AGTAACTGAAAGTATACCCC+GGG | + | chr8.3:36693610-36693629 | None:intergenic | 40.0% |
| ! | CGACTAGTCAAAGAATTGCA+GGG | - | chr8.3:36693550-36693569 | MS.gene71227:CDS | 40.0% |
| ! | TCGACTAGTCAAAGAATTGC+AGG | - | chr8.3:36693549-36693568 | MS.gene71227:CDS | 40.0% |
| !! | TTTCACGACACTTTGAAGCA+TGG | + | chr8.3:36694241-36694260 | None:intergenic | 40.0% |
| ACTCCCACCAAGAAAGAACT+TGG | + | chr8.3:36694824-36694843 | None:intergenic | 45.0% | |
| CTTTCTATTAGGGTGGGAAG+AGG | + | chr8.3:36694663-36694682 | None:intergenic | 45.0% | |
| GAGCTAATGCGTTTGGCTAT+TGG | - | chr8.3:36694087-36694106 | MS.gene71227:CDS | 45.0% | |
| GGATCATTTGATCCCAGAAG+TGG | + | chr8.3:36694803-36694822 | None:intergenic | 45.0% | |
| TTACAGCTTGCAGACTTGAC+CGG | - | chr8.3:36694063-36694082 | MS.gene71227:intron | 45.0% | |
| TTGTGGGTGTTTGCAGATAC+AGG | - | chr8.3:36693675-36693694 | MS.gene71227:intron | 45.0% | |
| ! | CACGTCACTTTCTATTAGGG+TGG | + | chr8.3:36694670-36694689 | None:intergenic | 45.0% |
| AAAGTATACCCCGGGTGAGT+AGG | + | chr8.3:36693602-36693621 | None:intergenic | 50.0% | |
| ATCTGCAGAGGCTGAAGAGA+AGG | + | chr8.3:36693796-36693815 | None:intergenic | 50.0% | |
| TACGCTTGTCGTGCCACATA+TGG | - | chr8.3:36694182-36694201 | MS.gene71227:CDS | 50.0% | |
| ! | AGCCAAACGCATTAGCTCTC+CGG | + | chr8.3:36694085-36694104 | None:intergenic | 50.0% |
| CTAAGACGAGCCTACTCACC+CGG | - | chr8.3:36693589-36693608 | MS.gene71227:intron | 55.0% | |
| GACCGGAGAGCTAATGCGTT+TGG | - | chr8.3:36694080-36694099 | MS.gene71227:CDS | 55.0% | |
| TAAGACGAGCCTACTCACCC+GGG | - | chr8.3:36693590-36693609 | MS.gene71227:intron | 55.0% | |
| ! | TGACTAGTCGAGAGACGTGC+TGG | + | chr8.3:36693540-36693559 | None:intergenic | 55.0% |
| AAGACGAGCCTACTCACCCG+GGG | - | chr8.3:36693591-36693610 | MS.gene71227:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 36693472 | 36694862 | 36693472 | ID=MS.gene71227 |
| chr8.3 | mRNA | 36693472 | 36694862 | 36693472 | ID=MS.gene71227.t1;Parent=MS.gene71227 |
| chr8.3 | exon | 36694722 | 36694862 | 36694722 | ID=MS.gene71227.t1.exon1;Parent=MS.gene71227.t1 |
| chr8.3 | CDS | 36694722 | 36694862 | 36694722 | ID=cds.MS.gene71227.t1;Parent=MS.gene71227.t1 |
| chr8.3 | exon | 36694494 | 36694643 | 36694494 | ID=MS.gene71227.t1.exon2;Parent=MS.gene71227.t1 |
| chr8.3 | CDS | 36694494 | 36694643 | 36694494 | ID=cds.MS.gene71227.t1;Parent=MS.gene71227.t1 |
| chr8.3 | exon | 36694064 | 36694265 | 36694064 | ID=MS.gene71227.t1.exon3;Parent=MS.gene71227.t1 |
| chr8.3 | CDS | 36694064 | 36694265 | 36694064 | ID=cds.MS.gene71227.t1;Parent=MS.gene71227.t1 |
| chr8.3 | exon | 36693472 | 36693572 | 36693472 | ID=MS.gene71227.t1.exon4;Parent=MS.gene71227.t1 |
| chr8.3 | CDS | 36693472 | 36693572 | 36693472 | ID=cds.MS.gene71227.t1;Parent=MS.gene71227.t1 |
| Gene Sequence |
| Protein sequence |
