Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015317.t1 | XP_003611464.1 | 99.4 | 166 | 1 | 0 | 1 | 166 | 1 | 166 | 3.50E-87 | 330.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015317.t1 | P16048 | 91.6 | 166 | 13 | 1 | 1 | 166 | 1 | 165 | 7.1e-81 | 301.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015317.t1 | G7JZW9 | 99.4 | 166 | 1 | 0 | 1 | 166 | 1 | 166 | 2.5e-87 | 330.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene015317 | MS.gene86926 | PPI |
| MS.gene015317 | MS.gene71609 | PPI |
| MS.gene015317 | MS.gene56419 | PPI |
| MS.gene015317 | MS.gene96697 | PPI |
| MS.gene015317 | MS.gene006904 | PPI |
| MS.gene015317 | MS.gene067556 | PPI |
| MS.gene015317 | MS.gene91314 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015317.t1 | MTR_5g014260 | 99.398 | 166 | 1 | 0 | 1 | 166 | 1 | 166 | 1.36e-120 | 337 |
| MS.gene015317.t1 | MTR_5g014260 | 99.200 | 125 | 1 | 0 | 1 | 125 | 1 | 125 | 2.29e-87 | 252 |
| MS.gene015317.t1 | MTR_4g084110 | 59.880 | 167 | 54 | 3 | 1 | 166 | 1 | 155 | 2.82e-69 | 207 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015317.t1 | AT1G32470 | 83.133 | 166 | 28 | 0 | 1 | 166 | 1 | 166 | 4.67e-94 | 270 |
| MS.gene015317.t1 | AT2G35370 | 83.133 | 166 | 27 | 1 | 1 | 166 | 1 | 165 | 2.24e-89 | 258 |
| MS.gene015317.t1 | AT2G35120 | 58.929 | 168 | 55 | 3 | 1 | 166 | 1 | 156 | 1.52e-64 | 195 |
Find 46 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAACTGAACCGCCAGGTTC+TGG | 0.305688 | 5.4:+8140800 | None:intergenic |
| ATCATCCATCCATCTTCATA+TGG | 0.339685 | 5.4:+8140313 | None:intergenic |
| GTTACAAAGGGTAACGGTTT+CGG | 0.348504 | 5.4:-8140782 | MS.gene015317:CDS |
| AAACATCTGGAGATGGAAAA+AGG | 0.352185 | 5.4:+8141455 | None:intergenic |
| TCAACAATCTCACCAGATAT+TGG | 0.373303 | 5.4:+8140719 | None:intergenic |
| ATCTCCAGATGTTTCTCAAC+TGG | 0.379569 | 5.4:-8141446 | MS.gene015317:intron |
| TCTTGGAGAGGTTGTGTTTG+TGG | 0.407524 | 5.4:-8140828 | MS.gene015317:CDS |
| CGGAGCTGTTGAAAGTGTTA+AGG | 0.409242 | 5.4:-8140762 | MS.gene015317:CDS |
| GATGTAAACTCTCCAATATC+TGG | 0.410884 | 5.4:-8140731 | MS.gene015317:CDS |
| ATCTGAATTAGAAGCCTTGT+TGG | 0.425384 | 5.4:-8140269 | MS.gene015317:CDS |
| AGTATGCATCTTCACATGAA+TGG | 0.426720 | 5.4:-8141322 | MS.gene015317:CDS |
| TAACAATGGCACTGAGGATG+TGG | 0.450009 | 5.4:-8141541 | None:intergenic |
| AGAAGAGAGTTTGAGAGCAT+TGG | 0.453540 | 5.4:+8141505 | None:intergenic |
| GATAAGCTCACCGGAAAACC+TGG | 0.464278 | 5.4:-8140689 | MS.gene015317:CDS |
| TGTTGGTTGCAGGACCATCT+TGG | 0.467787 | 5.4:-8140845 | MS.gene015317:intron |
| GAAGGCTCAGTAGCCACTAT+TGG | 0.468590 | 5.4:-8141290 | MS.gene015317:CDS |
| TCTGAATTAGAAGCCTTGTT+GGG | 0.485713 | 5.4:-8140268 | MS.gene015317:CDS |
| TCATCCATCCATCTTCATAT+GGG | 0.487407 | 5.4:+8140314 | None:intergenic |
| AATACCAGTTGAGAAACATC+TGG | 0.490504 | 5.4:+8141442 | None:intergenic |
| GTATGCATCTTCACATGAAT+GGG | 0.495142 | 5.4:-8141321 | MS.gene015317:CDS |
| AGAAGCCTTGTTGGGTGCAA+AGG | 0.506140 | 5.4:-8140260 | MS.gene015317:CDS |
| AATGAATGAATTACCAGGCC+AGG | 0.510526 | 5.4:+8140671 | None:intergenic |
| TTTGTGGAGCTTCCAGAACC+TGG | 0.512073 | 5.4:-8140812 | MS.gene015317:CDS |
| TCTAATTCAGATGGATCACT+TGG | 0.513292 | 5.4:+8140280 | None:intergenic |
| TGGTATCACTGACCATGCTC+AGG | 0.528384 | 5.4:-8141270 | MS.gene015317:intron |
| GCTCACCGGAAAACCTGGCC+TGG | 0.529026 | 5.4:-8140684 | MS.gene015317:intron |
| AACTCAAGCCCATATGAAGA+TGG | 0.530377 | 5.4:-8140322 | MS.gene015317:CDS |
| AGGGGAGAGATGTAATCTTG+AGG | 0.555174 | 5.4:+8141478 | None:intergenic |
| CATGAATGGGTGAAGCATGA+AGG | 0.562003 | 5.4:-8141308 | MS.gene015317:CDS |
| CCCTTTGTAACTGAACCGCC+AGG | 0.564034 | 5.4:+8140794 | None:intergenic |
| CAAGCCCATATGAAGATGGA+TGG | 0.567565 | 5.4:-8140318 | MS.gene015317:CDS |
| AACAAGGCTTCTAATTCAGA+TGG | 0.567704 | 5.4:+8140271 | None:intergenic |
| ACCTGGCGGTTCAGTTACAA+AGG | 0.580268 | 5.4:-8140795 | MS.gene015317:CDS |
| AGTTGAGAAACATCTGGAGA+TGG | 0.581853 | 5.4:+8141448 | None:intergenic |
| GTTGCAGGACCATCTTGGAG+AGG | 0.582318 | 5.4:-8140840 | MS.gene015317:intron |
| GGTTCAGTTACAAAGGGTAA+CGG | 0.588941 | 5.4:-8140788 | MS.gene015317:CDS |
| CAAACACAACCTCTCCAAGA+TGG | 0.593260 | 5.4:+8140831 | None:intergenic |
| GAGGTTAATGATAAGCTCAC+CGG | 0.595144 | 5.4:-8140698 | MS.gene015317:CDS |
| AATATCTGGTGAGATTGTTG+AGG | 0.595757 | 5.4:-8140717 | MS.gene015317:CDS |
| AACAATGGCACTGAGGATGT+GGG | 0.614850 | 5.4:-8141540 | None:intergenic |
| CCTGGCGGTTCAGTTACAAA+GGG | 0.619186 | 5.4:-8140794 | MS.gene015317:CDS |
| ATGGTCAGTGATACCAATAG+TGG | 0.628719 | 5.4:+8141277 | None:intergenic |
| GTACTCCTTTGCACCCAACA+AGG | 0.634212 | 5.4:+8140255 | None:intergenic |
| GCAGTTAACTAACCTGAGCA+TGG | 0.648601 | 5.4:+8141258 | None:intergenic |
| GTGGAGCTTCCAGAACCTGG+CGG | 0.691995 | 5.4:-8140809 | MS.gene015317:CDS |
| GAAGCTTAACAATGGCACTG+AGG | 0.716107 | 5.4:-8141547 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTACATTTATGAAAAATAAG+CGG | - | chr5.4:8141251-8141270 | MS.gene015317:intron | 20.0% |
| !!! | ATTCGAATTAGTTTGTTTTT+GGG | - | chr5.4:8140609-8140628 | MS.gene015317:intron | 20.0% |
| !!! | TATATAAATTTTTGCAGTGT+TGG | - | chr5.4:8140401-8140420 | MS.gene015317:intron | 20.0% |
| !!! | TATTCGAATTAGTTTGTTTT+TGG | - | chr5.4:8140608-8140627 | MS.gene015317:intron | 20.0% |
| ! | AATCATTACAACTTAAGTGA+AGG | - | chr5.4:8140862-8140881 | MS.gene015317:intron | 25.0% |
| ! | GTTTAATTAATTGCACTTGT+TGG | - | chr5.4:8140893-8140912 | MS.gene015317:intron | 25.0% |
| ! | TTAGACTTTAATTACTGTCT+AGG | - | chr5.4:8140721-8140740 | MS.gene015317:CDS | 25.0% |
| !! | GCTAGTATTTTATCTTTTGT+CGG | + | chr5.4:8140821-8140840 | None:intergenic | 25.0% |
| !!! | ATTAACTCACTCTCATTTTT+AGG | - | chr5.4:8141104-8141123 | MS.gene015317:intron | 25.0% |
| ACATATTGTGGAGAATGTTA+AGG | + | chr5.4:8140532-8140551 | None:intergenic | 30.0% | |
| ATTGAGCTAAGAGTTAAATG+AGG | - | chr5.4:8140774-8140793 | MS.gene015317:CDS | 30.0% | |
| CATTCAACTTGATAGGAAAA+AGG | + | chr5.4:8140355-8140374 | None:intergenic | 30.0% | |
| GAGTTAATGAATGAATTACC+AGG | + | chr5.4:8141092-8141111 | None:intergenic | 30.0% | |
| !! | TAAATTTTTGCAGTGTTGGA+TGG | - | chr5.4:8140405-8140424 | MS.gene015317:intron | 30.0% |
| !!! | GAATTAGTTTGTTTTTGGGT+CGG | - | chr5.4:8140613-8140632 | MS.gene015317:intron | 30.0% |
| AAACATCTGGAGATGGAAAA+AGG | + | chr5.4:8140303-8140322 | None:intergenic | 35.0% | |
| AACAAGGCTTCTAATTCAGA+TGG | + | chr5.4:8141487-8141506 | None:intergenic | 35.0% | |
| AAGACATAAGTGAACTAGAG+GGG | - | chr5.4:8141229-8141248 | MS.gene015317:intron | 35.0% | |
| AATACCAGTTGAGAAACATC+TGG | + | chr5.4:8140316-8140335 | None:intergenic | 35.0% | |
| AATATCTGGTGAGATTGTTG+AGG | - | chr5.4:8141038-8141057 | MS.gene015317:intron | 35.0% | |
| AGTATGCATCTTCACATGAA+TGG | - | chr5.4:8140433-8140452 | MS.gene015317:intron | 35.0% | |
| ATCATCCATCCATCTTCATA+TGG | + | chr5.4:8141445-8141464 | None:intergenic | 35.0% | |
| CAACTTGATAGGAAAAAGGA+GGG | + | chr5.4:8140351-8140370 | None:intergenic | 35.0% | |
| CCACATTCATTCAACTTGAT+AGG | + | chr5.4:8140362-8140381 | None:intergenic | 35.0% | |
| GAAGACATAAGTGAACTAGA+GGG | - | chr5.4:8141228-8141247 | MS.gene015317:intron | 35.0% | |
| GATGTAAACTCTCCAATATC+TGG | - | chr5.4:8141024-8141043 | MS.gene015317:intron | 35.0% | |
| GTATGCATCTTCACATGAAT+GGG | - | chr5.4:8140434-8140453 | MS.gene015317:intron | 35.0% | |
| TATTGTGGAGAATGTTAAGG+AGG | + | chr5.4:8140529-8140548 | None:intergenic | 35.0% | |
| TCAACAATCTCACCAGATAT+TGG | + | chr5.4:8141039-8141058 | None:intergenic | 35.0% | |
| TCAACTTGATAGGAAAAAGG+AGG | + | chr5.4:8140352-8140371 | None:intergenic | 35.0% | |
| TCATCCATCCATCTTCATAT+GGG | + | chr5.4:8141444-8141463 | None:intergenic | 35.0% | |
| TCTAATTCAGATGGATCACT+TGG | + | chr5.4:8141478-8141497 | None:intergenic | 35.0% | |
| TGAAGACATAAGTGAACTAG+AGG | - | chr5.4:8141227-8141246 | MS.gene015317:intron | 35.0% | |
| ! | ATCTGAATTAGAAGCCTTGT+TGG | - | chr5.4:8141486-8141505 | MS.gene015317:CDS | 35.0% |
| ! | GGAGTACACAAAATTTTGTG+AGG | - | chr5.4:8141516-8141535 | MS.gene015317:CDS | 35.0% |
| !! | CCTATCAAGTTGAATGAATG+TGG | - | chr5.4:8140359-8140378 | MS.gene015317:intron | 35.0% |
| !! | TCTGAATTAGAAGCCTTGTT+GGG | - | chr5.4:8141487-8141506 | MS.gene015317:CDS | 35.0% |
| AACTCAAGCCCATATGAAGA+TGG | - | chr5.4:8141433-8141452 | MS.gene015317:intron | 40.0% | |
| AATCTCACAGTTCACTTGTG+GGG | + | chr5.4:8140584-8140603 | None:intergenic | 40.0% | |
| AATGAATGAATTACCAGGCC+AGG | + | chr5.4:8141087-8141106 | None:intergenic | 40.0% | |
| AGTTGAGAAACATCTGGAGA+TGG | + | chr5.4:8140310-8140329 | None:intergenic | 40.0% | |
| ATCTCCAGATGTTTCTCAAC+TGG | - | chr5.4:8140309-8140328 | MS.gene015317:CDS | 40.0% | |
| ATCTGGAGATGGAAAAAGGA+GGG | + | chr5.4:8140299-8140318 | None:intergenic | 40.0% | |
| ATGGTCAGTGATACCAATAG+TGG | + | chr5.4:8140481-8140500 | None:intergenic | 40.0% | |
| CAATCTCACAGTTCACTTGT+GGG | + | chr5.4:8140585-8140604 | None:intergenic | 40.0% | |
| GAGGTTAATGATAAGCTCAC+CGG | - | chr5.4:8141057-8141076 | MS.gene015317:intron | 40.0% | |
| GGTTCAGTTACAAAGGGTAA+CGG | - | chr5.4:8140967-8140986 | MS.gene015317:intron | 40.0% | |
| TCAATCTCACAGTTCACTTG+TGG | + | chr5.4:8140586-8140605 | None:intergenic | 40.0% | |
| ! | AGAAGAGAGTTTGAGAGCAT+TGG | + | chr5.4:8140253-8140272 | None:intergenic | 40.0% |
| ! | GTTACAAAGGGTAACGGTTT+CGG | - | chr5.4:8140973-8140992 | MS.gene015317:intron | 40.0% |
| ! | TAATTGCACTTGTTGGTTGC+AGG | - | chr5.4:8140900-8140919 | MS.gene015317:intron | 40.0% |
| AGGGGAGAGATGTAATCTTG+AGG | + | chr5.4:8140280-8140299 | None:intergenic | 45.0% | |
| ATCTCACAGTTCACTTGTGG+GGG | + | chr5.4:8140583-8140602 | None:intergenic | 45.0% | |
| CAAACACAACCTCTCCAAGA+TGG | + | chr5.4:8140927-8140946 | None:intergenic | 45.0% | |
| CAAGCCCATATGAAGATGGA+TGG | - | chr5.4:8141437-8141456 | MS.gene015317:intron | 45.0% | |
| CATCTGGAGATGGAAAAAGG+AGG | + | chr5.4:8140300-8140319 | None:intergenic | 45.0% | |
| CATGAATGGGTGAAGCATGA+AGG | - | chr5.4:8140447-8140466 | MS.gene015317:intron | 45.0% | |
| CGCTTTGCATGGACATATTG+TGG | + | chr5.4:8140544-8140563 | None:intergenic | 45.0% | |
| GCAGTTAACTAACCTGAGCA+TGG | + | chr5.4:8140500-8140519 | None:intergenic | 45.0% | |
| TCTGGAGATGGAAAAAGGAG+GGG | + | chr5.4:8140298-8140317 | None:intergenic | 45.0% | |
| TCTTGGAGAGGTTGTGTTTG+TGG | - | chr5.4:8140927-8140946 | MS.gene015317:intron | 45.0% | |
| TGGAGAATGTTAAGGAGGAG+AGG | + | chr5.4:8140524-8140543 | None:intergenic | 45.0% | |
| ! | AATTACCAGGCCAGGTTTTC+CGG | + | chr5.4:8141079-8141098 | None:intergenic | 45.0% |
| !! | CAAAGCGCTCTGGCTTTAAA+TGG | - | chr5.4:8140557-8140576 | MS.gene015317:intron | 45.0% |
| !! | CGGAGCTGTTGAAAGTGTTA+AGG | - | chr5.4:8140993-8141012 | MS.gene015317:intron | 45.0% |
| ACCTGGCGGTTCAGTTACAA+AGG | - | chr5.4:8140960-8140979 | MS.gene015317:intron | 50.0% | |
| CCTGGCGGTTCAGTTACAAA+GGG | - | chr5.4:8140961-8140980 | MS.gene015317:intron | 50.0% | |
| GAAGGCTCAGTAGCCACTAT+TGG | - | chr5.4:8140465-8140484 | MS.gene015317:intron | 50.0% | |
| GATAAGCTCACCGGAAAACC+TGG | - | chr5.4:8141066-8141085 | MS.gene015317:intron | 50.0% | |
| GTACTCCTTTGCACCCAACA+AGG | + | chr5.4:8141503-8141522 | None:intergenic | 50.0% | |
| TGGTATCACTGACCATGCTC+AGG | - | chr5.4:8140485-8140504 | MS.gene015317:intron | 50.0% | |
| TTTGTGGAGCTTCCAGAACC+TGG | - | chr5.4:8140943-8140962 | MS.gene015317:intron | 50.0% | |
| ! | TAAAGCCAGAGCGCTTTGCA+TGG | + | chr5.4:8140555-8140574 | None:intergenic | 50.0% |
| ! | TATGTCCATGCAAAGCGCTC+TGG | - | chr5.4:8140547-8140566 | MS.gene015317:intron | 50.0% |
| ! | TGTTGGTTGCAGGACCATCT+TGG | - | chr5.4:8140910-8140929 | MS.gene015317:intron | 50.0% |
| !! | AGAAGCCTTGTTGGGTGCAA+AGG | - | chr5.4:8141495-8141514 | MS.gene015317:CDS | 50.0% |
| CCCTTTGTAACTGAACCGCC+AGG | + | chr5.4:8140964-8140983 | None:intergenic | 55.0% | |
| GTAACTGAACCGCCAGGTTC+TGG | + | chr5.4:8140958-8140977 | None:intergenic | 55.0% | |
| GTTGCAGGACCATCTTGGAG+AGG | - | chr5.4:8140915-8140934 | MS.gene015317:intron | 55.0% | |
| GTGGAGCTTCCAGAACCTGG+CGG | - | chr5.4:8140946-8140965 | MS.gene015317:intron | 60.0% | |
| GCTCACCGGAAAACCTGGCC+TGG | - | chr5.4:8141071-8141090 | MS.gene015317:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 8140219 | 8141558 | 8140219 | ID=MS.gene015317 |
| chr5.4 | mRNA | 8140219 | 8141558 | 8140219 | ID=MS.gene015317.t1;Parent=MS.gene015317 |
| chr5.4 | exon | 8141447 | 8141558 | 8141447 | ID=MS.gene015317.t1.exon1;Parent=MS.gene015317.t1 |
| chr5.4 | CDS | 8141447 | 8141558 | 8141447 | ID=cds.MS.gene015317.t1;Parent=MS.gene015317.t1 |
| chr5.4 | exon | 8141271 | 8141359 | 8141271 | ID=MS.gene015317.t1.exon2;Parent=MS.gene015317.t1 |
| chr5.4 | CDS | 8141271 | 8141359 | 8141271 | ID=cds.MS.gene015317.t1;Parent=MS.gene015317.t1 |
| chr5.4 | exon | 8140685 | 8140855 | 8140685 | ID=MS.gene015317.t1.exon3;Parent=MS.gene015317.t1 |
| chr5.4 | CDS | 8140685 | 8140855 | 8140685 | ID=cds.MS.gene015317.t1;Parent=MS.gene015317.t1 |
| chr5.4 | exon | 8140219 | 8140347 | 8140219 | ID=MS.gene015317.t1.exon4;Parent=MS.gene015317.t1 |
| chr5.4 | CDS | 8140219 | 8140347 | 8140219 | ID=cds.MS.gene015317.t1;Parent=MS.gene015317.t1 |
| Gene Sequence |
| Protein sequence |
