AlfalfaGEDB | MS.gene86926

M. sativa cultivar XinJiangDaYe / MS.gene86926

KEGG: K23735
GO: GO:0003674 GO:0003824 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009249 GO:0009987 GO:0016740 GO:0016746 GO:0016747 GO:0018065 GO:0018193 GO:0018205 GO:0019538 GO:0033819 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0051604 GO:0071704 GO:1901564
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86926.t1	XP_013469979.1	97.4	235	6	0	1	235	1	235	5.50E-131	476.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86926.t1	Q9SXP7	74.0	235	61	0	1	235	1	235	1.8e-101	370.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86926.t1	A0A072VQE6	97.4	235	6	0	1	235	1	235	4.0e-131	476.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049217	MS.gene86926	0.81194	5.44E-51	-1.69E-46
MS.gene052271	MS.gene86926	0.820157	7.95E-53	-1.69E-46
MS.gene052379	MS.gene86926	0.818907	1.53E-52	-1.69E-46
MS.gene053322	MS.gene86926	0.80921	2.12E-50	-1.69E-46
MS.gene053813	MS.gene86926	0.812175	4.84E-51	-1.69E-46
MS.gene055636	MS.gene86926	0.817362	3.43E-52	-1.69E-46
MS.gene055647	MS.gene86926	0.800327	1.52E-48	-1.69E-46
MS.gene05592	MS.gene86926	0.822546	2.24E-53	-1.69E-46
MS.gene057146	MS.gene86926	0.806414	8.32E-50	-1.69E-46
MS.gene057416	MS.gene86926	0.806688	7.28E-50	-1.69E-46
MS.gene059029	MS.gene86926	0.81442	1.55E-51	-1.69E-46
MS.gene059711	MS.gene86926	0.818591	1.81E-52	-1.69E-46
MS.gene060818	MS.gene86926	0.838924	2.15E-57	-1.69E-46
MS.gene061134	MS.gene86926	0.825242	5.21E-54	-1.69E-46
MS.gene061171	MS.gene86926	0.817121	3.89E-52	-1.69E-46
MS.gene061200	MS.gene86926	0.807666	4.52E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene015317	MS.gene86926	PPI
MS.gene86926	MS.gene059677	PPI
MS.gene09661	MS.gene86926	PPI
MS.gene86926	MS.gene21474	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86926.t1	MTR_1g106190	97.447	235	6	0	1	235	1	235	4.20e-175	480
MS.gene86926.t1	MTR_3g111580	32.143	168	98	7	1	156	44	207	4.08e-16	75.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86926.t1	AT1G04640	74.043	235	61	0	1	235	1	235	1.56e-132	373
MS.gene86926.t1	AT1G04640	74.043	235	61	0	1	235	1	235	1.56e-132	373
MS.gene86926.t1	AT1G47578	35.323	201	117	4	23	214	70	266	5.37e-34	123
MS.gene86926.t1	AT4G31050	35.047	214	122	6	14	214	54	263	6.69e-34	123

Find 57 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACCCTTGAGGTATGGAAAA+TGG	0.246851	1.2:+79143682	MS.gene86926:CDS
AATGGGAGTCGTCAACTATT+TGG	0.263377	1.2:+79143700	MS.gene86926:CDS
ATCCTGATTTAAACTACTTT+AGG	0.329725	1.2:+79144164	MS.gene86926:CDS
ATTAATCCCTCAGTCAGAAT+TGG	0.351556	1.2:+79143847	MS.gene86926:CDS
GGAGGAGACATTACATTTCA+TGG	0.377047	1.2:+79143905	MS.gene86926:CDS
CCTGGACGGAAGTGCGAGAC+AGG	0.381350	1.2:+79144055	MS.gene86926:CDS
ATTGAACTGGCAGGTCTATA+CGG	0.398561	1.2:+79144019	MS.gene86926:CDS
TGCCTAAAGTAGTTTAAATC+AGG	0.405093	1.2:-79144166	None:intergenic
CTCTTGTATTATTTCTTCTG+TGG	0.407985	1.2:-79144257	None:intergenic
GTGTCCCGGAGCGATATAAT+AGG	0.424169	1.2:-79143950	None:intergenic
TTGGTTATGATAACCTGGTT+TGG	0.424805	1.2:+79144308	MS.gene86926:CDS
TGAGTTAACTATGATTGAAC+TGG	0.440439	1.2:+79144006	MS.gene86926:CDS
ACCCTTGAGGTATGGAAAAT+GGG	0.459093	1.2:+79143683	MS.gene86926:CDS
GTATTATTTCTTCTGTGGGA+AGG	0.467664	1.2:-79144252	None:intergenic
TCGCTCCGGGACACGGGACT+TGG	0.469720	1.2:+79143959	MS.gene86926:CDS
CTTTGCGAACCCTTGAGGTA+TGG	0.471055	1.2:+79143675	MS.gene86926:CDS
TACGGTGTGAAAGCCTGTCC+TGG	0.472535	1.2:+79144037	MS.gene86926:CDS
CTCGCACTTCCGTCCAGGAC+AGG	0.473460	1.2:-79144050	None:intergenic
AGAAATAATACAAGAGCAGT+TGG	0.475927	1.2:+79144264	MS.gene86926:CDS
TCGAATGCAATCACTTCTCA+TGG	0.484306	1.2:+79144124	MS.gene86926:CDS
GAAAGAAAGATAGGTGCAAT+TGG	0.488210	1.2:+79144091	MS.gene86926:CDS
TATATCCTATTATATCGCTC+CGG	0.489442	1.2:+79143945	MS.gene86926:CDS
ACTGCAAGAAAAGCTAGTCT+TGG	0.489810	1.2:+79143733	MS.gene86926:CDS
TCTTGTATTATTTCTTCTGT+GGG	0.491663	1.2:-79144256	None:intergenic
TATTATATCGCTCCGGGACA+CGG	0.491774	1.2:+79143952	MS.gene86926:CDS
AGATAGGTGCAATTGGTGTT+CGG	0.495508	1.2:+79144098	MS.gene86926:CDS
GGAAGTGCGAGACAGGGGTG+TGG	0.505514	1.2:+79144062	MS.gene86926:CDS
CAGTCAGAATTGGAACAGAT+TGG	0.505622	1.2:+79143857	MS.gene86926:CDS
CCTGTCTCGCACTTCCGTCC+AGG	0.510685	1.2:-79144055	None:intergenic
ATATCCTATTATATCGCTCC+GGG	0.515465	1.2:+79143946	MS.gene86926:CDS
CGATATAATAGGATATAAAA+CGG	0.517995	1.2:-79143939	None:intergenic
TTGCCAACTGTGTATGTAGG+AGG	0.525301	1.2:-79143803	None:intergenic
TGCGAGACAGGGGTGTGGGT+TGG	0.535248	1.2:+79144067	MS.gene86926:CDS
TAGCACCAAGTCCCGTGTCC+CGG	0.536717	1.2:-79143964	None:intergenic
TGGGTTGGAGAAAGAAAGAT+AGG	0.538417	1.2:+79144082	MS.gene86926:CDS
TGCAATCACTTCTCATGGGA+TGG	0.538484	1.2:+79144129	MS.gene86926:CDS
ATTATATCGCTCCGGGACAC+GGG	0.540311	1.2:+79143953	MS.gene86926:CDS
TTATGATAACCTGGTTTGGA+AGG	0.544272	1.2:+79144312	MS.gene86926:CDS
TAATAGGATATAAAACGGCT+TGG	0.546253	1.2:-79143934	None:intergenic
TGATAACCTGGTTTGGAAGG+AGG	0.556596	1.2:+79144315	MS.gene86926:CDS
TCTGTTCCAATTCTGACTGA+GGG	0.560843	1.2:-79143853	None:intergenic
AAGCATCCTCCTTCCAAACC+AGG	0.571828	1.2:-79144321	None:intergenic
GGATATAAAACGGCTTGGTG+TGG	0.578369	1.2:-79143929	None:intergenic
ATCTGTTCCAATTCTGACTG+AGG	0.581218	1.2:-79143854	None:intergenic
GAAGTGCGAGACAGGGGTGT+GGG	0.583742	1.2:+79144063	MS.gene86926:CDS
CACCCTCCTACATACACAGT+TGG	0.584308	1.2:+79143800	MS.gene86926:CDS
CTGGACGGAAGTGCGAGACA+GGG	0.584751	1.2:+79144056	MS.gene86926:CDS
TTAACTATGATTGAACTGGC+AGG	0.588243	1.2:+79144010	MS.gene86926:CDS
TGCCAACTGTGTATGTAGGA+GGG	0.592743	1.2:-79143802	None:intergenic
GAGGGCTTTGCGAACCCTTG+AGG	0.595771	1.2:+79143670	MS.gene86926:CDS
TGGACGGAAGTGCGAGACAG+GGG	0.609441	1.2:+79144057	MS.gene86926:CDS
CGAATGCAATCACTTCTCAT+GGG	0.616033	1.2:+79144125	MS.gene86926:CDS
GTGTGAAAGCCTGTCCTGGA+CGG	0.616171	1.2:+79144041	MS.gene86926:CDS
TCTTTATCAGCAATTCCACA+CGG	0.675103	1.2:-79144196	None:intergenic
TTTAGGCACATTGTACCGTG+TGG	0.676153	1.2:+79144181	MS.gene86926:CDS
CTTCACTACACACAACGTGG+AGG	0.719716	1.2:+79143887	MS.gene86926:CDS
GAGCTTCACTACACACAACG+TGG	0.727037	1.2:+79143884	MS.gene86926:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CGATATAATAGGATATAAAA+CGG	-	chr1.2:79143942-79143961	None:intergenic	20.0%
!	ATCCTGATTTAAACTACTTT+AGG	+	chr1.2:79144164-79144183	MS.gene86926:CDS	25.0%
!!	TCTTGTATTATTTCTTCTGT+GGG	-	chr1.2:79144259-79144278	None:intergenic	25.0%
!!!	AATTTTTGGTTATGATAACC+TGG	+	chr1.2:79144303-79144322	MS.gene86926:CDS	25.0%
!!!	TCTTGTTTTGCAAGAATTTT+TGG	+	chr1.2:79144289-79144308	MS.gene86926:CDS	25.0%
	AGAAATAATACAAGAGCAGT+TGG	+	chr1.2:79144264-79144283	MS.gene86926:CDS	30.0%
	TAATAGGATATAAAACGGCT+TGG	-	chr1.2:79143937-79143956	None:intergenic	30.0%
	TATATCCTATTATATCGCTC+CGG	+	chr1.2:79143945-79143964	MS.gene86926:CDS	30.0%
	TGAGTTAACTATGATTGAAC+TGG	+	chr1.2:79144006-79144025	MS.gene86926:CDS	30.0%
	TGCCTAAAGTAGTTTAAATC+AGG	-	chr1.2:79144169-79144188	None:intergenic	30.0%
!	CTCTTGTATTATTTCTTCTG+TGG	-	chr1.2:79144260-79144279	None:intergenic	30.0%
	ATATCCTATTATATCGCTCC+GGG	+	chr1.2:79143946-79143965	MS.gene86926:CDS	35.0%
	ATTAATCCCTCAGTCAGAAT+TGG	+	chr1.2:79143847-79143866	MS.gene86926:CDS	35.0%
	GAAAGAAAGATAGGTGCAAT+TGG	+	chr1.2:79144091-79144110	MS.gene86926:CDS	35.0%
	TCTTTATCAGCAATTCCACA+CGG	-	chr1.2:79144199-79144218	None:intergenic	35.0%
	TTAACTATGATTGAACTGGC+AGG	+	chr1.2:79144010-79144029	MS.gene86926:CDS	35.0%
	TTGGTTATGATAACCTGGTT+TGG	+	chr1.2:79144308-79144327	MS.gene86926:CDS	35.0%
!	GTATTATTTCTTCTGTGGGA+AGG	-	chr1.2:79144255-79144274	None:intergenic	35.0%
!!	TTATGATAACCTGGTTTGGA+AGG	+	chr1.2:79144312-79144331	MS.gene86926:CDS	35.0%
	AACCCTTGAGGTATGGAAAA+TGG	+	chr1.2:79143682-79143701	MS.gene86926:CDS	40.0%
	AATGGGAGTCGTCAACTATT+TGG	+	chr1.2:79143700-79143719	MS.gene86926:CDS	40.0%
	ACCCTTGAGGTATGGAAAAT+GGG	+	chr1.2:79143683-79143702	MS.gene86926:CDS	40.0%
	ATCTGTTCCAATTCTGACTG+AGG	-	chr1.2:79143857-79143876	None:intergenic	40.0%
	ATTGAACTGGCAGGTCTATA+CGG	+	chr1.2:79144019-79144038	MS.gene86926:CDS	40.0%
	CAGTCAGAATTGGAACAGAT+TGG	+	chr1.2:79143857-79143876	MS.gene86926:CDS	40.0%
	CGAATGCAATCACTTCTCAT+GGG	+	chr1.2:79144125-79144144	MS.gene86926:CDS	40.0%
	GGAGGAGACATTACATTTCA+TGG	+	chr1.2:79143905-79143924	MS.gene86926:CDS	40.0%
	TCGAATGCAATCACTTCTCA+TGG	+	chr1.2:79144124-79144143	MS.gene86926:CDS	40.0%
	TCTGTTCCAATTCTGACTGA+GGG	-	chr1.2:79143856-79143875	None:intergenic	40.0%
!	ACTGCAAGAAAAGCTAGTCT+TGG	+	chr1.2:79143733-79143752	MS.gene86926:CDS	40.0%
!	AGATAGGTGCAATTGGTGTT+CGG	+	chr1.2:79144098-79144117	MS.gene86926:CDS	40.0%
!	CTTTTGCCAACTGTGTATGT+AGG	-	chr1.2:79143809-79143828	None:intergenic	40.0%
!	TCCCATTTTCCATACCTCAA+GGG	-	chr1.2:79143687-79143706	None:intergenic	40.0%
!	TGGGTTGGAGAAAGAAAGAT+AGG	+	chr1.2:79144082-79144101	MS.gene86926:CDS	40.0%
	TATTATATCGCTCCGGGACA+CGG	+	chr1.2:79143952-79143971	MS.gene86926:CDS	45.0%
	TGCAATCACTTCTCATGGGA+TGG	+	chr1.2:79144129-79144148	MS.gene86926:CDS	45.0%
	TGCCAACTGTGTATGTAGGA+GGG	-	chr1.2:79143805-79143824	None:intergenic	45.0%
	TTGCCAACTGTGTATGTAGG+AGG	-	chr1.2:79143806-79143825	None:intergenic	45.0%
	TTTAGGCACATTGTACCGTG+TGG	+	chr1.2:79144181-79144200	MS.gene86926:CDS	45.0%
!	CTCCCATTTTCCATACCTCA+AGG	-	chr1.2:79143688-79143707	None:intergenic	45.0%
!!	GGATATAAAACGGCTTGGTG+TGG	-	chr1.2:79143932-79143951	None:intergenic	45.0%
!!	TGATAACCTGGTTTGGAAGG+AGG	+	chr1.2:79144315-79144334	MS.gene86926:CDS	45.0%
	AAGCATCCTCCTTCCAAACC+AGG	-	chr1.2:79144324-79144343	None:intergenic	50.0%
	ATTATATCGCTCCGGGACAC+GGG	+	chr1.2:79143953-79143972	MS.gene86926:CDS	50.0%
	CACCCTCCTACATACACAGT+TGG	+	chr1.2:79143800-79143819	MS.gene86926:CDS	50.0%
	CTTCACTACACACAACGTGG+AGG	+	chr1.2:79143887-79143906	MS.gene86926:CDS	50.0%
	CTTTGCGAACCCTTGAGGTA+TGG	+	chr1.2:79143675-79143694	MS.gene86926:CDS	50.0%
	GAGCTTCACTACACACAACG+TGG	+	chr1.2:79143884-79143903	MS.gene86926:CDS	50.0%
	GTGTCCCGGAGCGATATAAT+AGG	-	chr1.2:79143953-79143972	None:intergenic	50.0%
	GTGTGAAAGCCTGTCCTGGA+CGG	+	chr1.2:79144041-79144060	MS.gene86926:CDS	55.0%
	TACGGTGTGAAAGCCTGTCC+TGG	+	chr1.2:79144037-79144056	MS.gene86926:CDS	55.0%
	GAGGGCTTTGCGAACCCTTG+AGG	+	chr1.2:79143670-79143689	MS.gene86926:CDS	60.0%
	TAGCACCAAGTCCCGTGTCC+CGG	-	chr1.2:79143967-79143986	None:intergenic	60.0%
!	CTGGACGGAAGTGCGAGACA+GGG	+	chr1.2:79144056-79144075	MS.gene86926:CDS	60.0%
!	GAAGTGCGAGACAGGGGTGT+GGG	+	chr1.2:79144063-79144082	MS.gene86926:CDS	60.0%
!	TGGACGGAAGTGCGAGACAG+GGG	+	chr1.2:79144057-79144076	MS.gene86926:CDS	60.0%
	CCTGTCTCGCACTTCCGTCC+AGG	-	chr1.2:79144058-79144077	None:intergenic	65.0%
	CTCGCACTTCCGTCCAGGAC+AGG	-	chr1.2:79144053-79144072	None:intergenic	65.0%
!	CCTGGACGGAAGTGCGAGAC+AGG	+	chr1.2:79144055-79144074	MS.gene86926:CDS	65.0%
!	GGAAGTGCGAGACAGGGGTG+TGG	+	chr1.2:79144062-79144081	MS.gene86926:CDS	65.0%
!	TGCGAGACAGGGGTGTGGGT+TGG	+	chr1.2:79144067-79144086	MS.gene86926:CDS	65.0%
	TCGCTCCGGGACACGGGACT+TGG	+	chr1.2:79143959-79143978	MS.gene86926:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	79143668	79144375	79143668	ID=MS.gene86926
chr1.2	mRNA	79143668	79144375	79143668	ID=MS.gene86926.t1;Parent=MS.gene86926
chr1.2	exon	79143668	79144375	79143668	ID=MS.gene86926.t1.exon1;Parent=MS.gene86926.t1
chr1.2	CDS	79143668	79144375	79143668	ID=cds.MS.gene86926.t1;Parent=MS.gene86926.t1

Gene Sequence

>MS.gene86926

ATGAGGGCTTTGCGAACCCTTGAGGTATGGAAAATGGGAGTCGTCAACTATTTGGATGCGTTAAAACTGCAAGAAAAGCTAGTCTTGGATAGAAAATCTCATAAGAGATGTGATACTCTTCTATCTTTGCAACACCCTCCTACATACACAGTTGGCAAAAGACAAACTGTTCATAATTTATTAATCCCTCAGTCAGAATTGGAACAGATTGGCGCTGAGCTTCACTACACACAACGTGGAGGAGACATTACATTTCATGGTCCACACCAAGCCGTTTTATATCCTATTATATCGCTCCGGGACACGGGACTTGGTGCTAGAAAATTTGTAGAAAAAATTGAGTTAACTATGATTGAACTGGCAGGTCTATACGGTGTGAAAGCCTGTCCTGGACGGAAGTGCGAGACAGGGGTGTGGGTTGGAGAAAGAAAGATAGGTGCAATTGGTGTTCGGATATCGAATGCAATCACTTCTCATGGGATGGCGTTTAACATTGATCCTGATTTAAACTACTTTAGGCACATTGTACCGTGTGGAATTGCTGATAAAGAAGTGACATCTTTGAGAAGAGAGACAGATTTTGACCTTCCCACAGAAGAAATAATACAAGAGCAGTTGGTTTCTTGTTTTGCAAGAATTTTTGGTTATGATAACCTGGTTTGGAAGGAGGATGCTTCTATATTATCTGATAAAAATGAAACTGAATGA

Protein sequence

>MS.gene86926.t1

MRALRTLEVWKMGVVNYLDALKLQEKLVLDRKSHKRCDTLLSLQHPPTYTVGKRQTVHNLLIPQSELEQIGAELHYTQRGGDITFHGPHQAVLYPIISLRDTGLGARKFVEKIELTMIELAGLYGVKACPGRKCETGVWVGERKIGAIGVRISNAITSHGMAFNIDPDLNYFRHIVPCGIADKEVTSLRRETDFDLPTEEIIQEQLVSCFARIFGYDNLVWKEDASILSDKNETE