Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71609.t1 | XP_013469979.1 | 97 | 235 | 7 | 0 | 1 | 235 | 1 | 235 | 2.10E-130 | 474.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71609.t1 | Q9SXP7 | 74.0 | 235 | 61 | 0 | 1 | 235 | 1 | 235 | 1.8e-101 | 370.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71609.t1 | A0A072VQE6 | 97.0 | 235 | 7 | 0 | 1 | 235 | 1 | 235 | 1.5e-130 | 474.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene71609 | 0.8251 | 5.63E-54 | -1.69E-46 |
| MS.gene049171 | MS.gene71609 | 0.833787 | 4.36E-56 | -1.69E-46 |
| MS.gene049217 | MS.gene71609 | 0.859149 | 5.05E-63 | -1.69E-46 |
| MS.gene049222 | MS.gene71609 | 0.852167 | 5.51E-61 | -1.69E-46 |
| MS.gene049285 | MS.gene71609 | 0.85388 | 1.78E-61 | -1.69E-46 |
| MS.gene049320 | MS.gene71609 | 0.833683 | 4.63E-56 | -1.69E-46 |
| MS.gene049425 | MS.gene71609 | -0.80854 | 2.95E-50 | -1.69E-46 |
| MS.gene049427 | MS.gene71609 | 0.806341 | 8.62E-50 | -1.69E-46 |
| MS.gene049609 | MS.gene71609 | 0.864856 | 9.00E-65 | -1.69E-46 |
| MS.gene049805 | MS.gene71609 | 0.867434 | 1.37E-65 | -1.69E-46 |
| MS.gene05010 | MS.gene71609 | 0.818636 | 1.77E-52 | -1.69E-46 |
| MS.gene050163 | MS.gene71609 | 0.814551 | 1.45E-51 | -1.69E-46 |
| MS.gene050165 | MS.gene71609 | 0.80366 | 3.14E-49 | -1.69E-46 |
| MS.gene050325 | MS.gene71609 | -0.801136 | 1.04E-48 | -1.69E-46 |
| MS.gene050327 | MS.gene71609 | -0.817898 | 2.60E-52 | -1.69E-46 |
| MS.gene050333 | MS.gene71609 | 0.821278 | 4.40E-53 | -1.69E-46 |
| MS.gene050432 | MS.gene71609 | 0.811344 | 7.34E-51 | -1.69E-46 |
| MS.gene050504 | MS.gene71609 | 0.810655 | 1.03E-50 | -1.69E-46 |
| MS.gene050518 | MS.gene71609 | 0.81223 | 4.71E-51 | -1.69E-46 |
| MS.gene050538 | MS.gene71609 | 0.812052 | 5.15E-51 | -1.69E-46 |
| MS.gene050593 | MS.gene71609 | 0.849934 | 2.35E-60 | -1.69E-46 |
| MS.gene050612 | MS.gene71609 | 0.80982 | 1.57E-50 | -1.69E-46 |
| MS.gene050662 | MS.gene71609 | 0.807215 | 5.64E-50 | -1.69E-46 |
| MS.gene050670 | MS.gene71609 | 0.816228 | 6.16E-52 | -1.69E-46 |
| MS.gene050686 | MS.gene71609 | 0.841944 | 3.50E-58 | -1.69E-46 |
| MS.gene050699 | MS.gene71609 | 0.807743 | 4.35E-50 | -1.69E-46 |
| MS.gene050747 | MS.gene71609 | 0.837615 | 4.68E-57 | -1.69E-46 |
| MS.gene050770 | MS.gene71609 | 0.801949 | 7.07E-49 | -1.69E-46 |
| MS.gene050772 | MS.gene71609 | 0.884849 | 1.35E-71 | -1.69E-46 |
| MS.gene05080 | MS.gene71609 | 0.80531 | 1.42E-49 | -1.69E-46 |
| MS.gene05081 | MS.gene71609 | 0.812566 | 3.97E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene71609 | 0.802131 | 6.49E-49 | -1.69E-46 |
| MS.gene050871 | MS.gene71609 | 0.80196 | 7.04E-49 | -1.69E-46 |
| MS.gene050950 | MS.gene71609 | 0.829408 | 5.23E-55 | -1.69E-46 |
| MS.gene05100 | MS.gene71609 | 0.804492 | 2.11E-49 | -1.69E-46 |
| MS.gene05105 | MS.gene71609 | 0.841982 | 3.42E-58 | -1.69E-46 |
| MS.gene051113 | MS.gene71609 | -0.827391 | 1.61E-54 | -1.69E-46 |
| MS.gene051167 | MS.gene71609 | -0.808948 | 2.41E-50 | -1.69E-46 |
| MS.gene051228 | MS.gene71609 | 0.811018 | 8.63E-51 | -1.69E-46 |
| MS.gene051387 | MS.gene71609 | 0.850717 | 1.42E-60 | -1.69E-46 |
| MS.gene051666 | MS.gene71609 | 0.856305 | 3.52E-62 | -1.69E-46 |
| MS.gene051692 | MS.gene71609 | 0.809379 | 1.95E-50 | -1.69E-46 |
| MS.gene051694 | MS.gene71609 | 0.853848 | 1.82E-61 | -1.69E-46 |
| MS.gene051810 | MS.gene71609 | 0.811628 | 6.37E-51 | -1.69E-46 |
| MS.gene051838 | MS.gene71609 | 0.803718 | 3.05E-49 | -1.69E-46 |
| MS.gene051858 | MS.gene71609 | 0.866785 | 2.21E-65 | -1.69E-46 |
| MS.gene051877 | MS.gene71609 | 0.804069 | 2.58E-49 | -1.69E-46 |
| MS.gene052271 | MS.gene71609 | 0.822974 | 1.78E-53 | -1.69E-46 |
| MS.gene052322 | MS.gene71609 | 0.808045 | 3.76E-50 | -1.69E-46 |
| MS.gene052379 | MS.gene71609 | 0.862848 | 3.79E-64 | -1.69E-46 |
| MS.gene052421 | MS.gene71609 | 0.844215 | 8.68E-59 | -1.69E-46 |
| MS.gene052484 | MS.gene71609 | 0.825241 | 5.22E-54 | -1.69E-46 |
| MS.gene052501 | MS.gene71609 | 0.802319 | 5.93E-49 | -1.69E-46 |
| MS.gene052503 | MS.gene71609 | 0.804276 | 2.34E-49 | -1.69E-46 |
| MS.gene052506 | MS.gene71609 | 0.821368 | 4.19E-53 | -1.69E-46 |
| MS.gene052605 | MS.gene71609 | 0.812015 | 5.24E-51 | -1.69E-46 |
| MS.gene052615 | MS.gene71609 | 0.8273 | 1.69E-54 | -1.69E-46 |
| MS.gene052820 | MS.gene71609 | 0.802103 | 6.58E-49 | -1.69E-46 |
| MS.gene052822 | MS.gene71609 | 0.82595 | 3.55E-54 | -1.69E-46 |
| MS.gene052870 | MS.gene71609 | 0.819829 | 9.46E-53 | -1.69E-46 |
| MS.gene052872 | MS.gene71609 | 0.820443 | 6.84E-53 | -1.69E-46 |
| MS.gene053009 | MS.gene71609 | 0.820333 | 7.25E-53 | -1.69E-46 |
| MS.gene053103 | MS.gene71609 | 0.833501 | 5.14E-56 | -1.69E-46 |
| MS.gene053104 | MS.gene71609 | 0.850242 | 1.93E-60 | -1.69E-46 |
| MS.gene053203 | MS.gene71609 | 0.809052 | 2.29E-50 | -1.69E-46 |
| MS.gene053207 | MS.gene71609 | 0.857927 | 1.17E-62 | -1.69E-46 |
| MS.gene053213 | MS.gene71609 | 0.860788 | 1.62E-63 | -1.69E-46 |
| MS.gene053286 | MS.gene71609 | 0.849269 | 3.60E-60 | -1.69E-46 |
| MS.gene053322 | MS.gene71609 | 0.857049 | 2.13E-62 | -1.69E-46 |
| MS.gene053449 | MS.gene71609 | 0.825354 | 4.91E-54 | -1.69E-46 |
| MS.gene053479 | MS.gene71609 | 0.800472 | 1.42E-48 | -1.69E-46 |
| MS.gene053574 | MS.gene71609 | 0.851987 | 6.20E-61 | -1.69E-46 |
| MS.gene053632 | MS.gene71609 | 0.846788 | 1.74E-59 | -1.69E-46 |
| MS.gene053813 | MS.gene71609 | 0.817495 | 3.20E-52 | -1.69E-46 |
| MS.gene053824 | MS.gene71609 | 0.83697 | 6.85E-57 | -1.69E-46 |
| MS.gene053916 | MS.gene71609 | 0.846122 | 2.65E-59 | -1.69E-46 |
| MS.gene054003 | MS.gene71609 | 0.831648 | 1.48E-55 | -1.69E-46 |
| MS.gene054408 | MS.gene71609 | 0.802479 | 5.50E-49 | -1.69E-46 |
| MS.gene054595 | MS.gene71609 | 0.81482 | 1.27E-51 | -1.69E-46 |
| MS.gene054600 | MS.gene71609 | 0.829831 | 4.13E-55 | -1.69E-46 |
| MS.gene054642 | MS.gene71609 | 0.861117 | 1.29E-63 | -1.69E-46 |
| MS.gene054802 | MS.gene71609 | 0.807373 | 5.22E-50 | -1.69E-46 |
| MS.gene054803 | MS.gene71609 | 0.801797 | 7.60E-49 | -1.69E-46 |
| MS.gene054804 | MS.gene71609 | 0.808098 | 3.66E-50 | -1.69E-46 |
| MS.gene054927 | MS.gene71609 | 0.829298 | 5.57E-55 | -1.69E-46 |
| MS.gene05494 | MS.gene71609 | 0.806926 | 6.49E-50 | -1.69E-46 |
| MS.gene054954 | MS.gene71609 | 0.812313 | 4.52E-51 | -1.69E-46 |
| MS.gene05505 | MS.gene71609 | 0.876629 | 1.20E-68 | -1.69E-46 |
| MS.gene055206 | MS.gene71609 | 0.874156 | 8.42E-68 | -1.69E-46 |
| MS.gene055212 | MS.gene71609 | 0.813079 | 3.07E-51 | -1.69E-46 |
| MS.gene055224 | MS.gene71609 | 0.818304 | 2.10E-52 | -1.69E-46 |
| MS.gene055289 | MS.gene71609 | 0.846492 | 2.10E-59 | -1.69E-46 |
| MS.gene055362 | MS.gene71609 | 0.84578 | 3.28E-59 | -1.69E-46 |
| MS.gene055499 | MS.gene71609 | 0.815366 | 9.59E-52 | -1.69E-46 |
| MS.gene055502 | MS.gene71609 | 0.846754 | 1.78E-59 | -1.69E-46 |
| MS.gene055577 | MS.gene71609 | 0.833586 | 4.90E-56 | -1.69E-46 |
| MS.gene055636 | MS.gene71609 | 0.82383 | 1.12E-53 | -1.69E-46 |
| MS.gene055638 | MS.gene71609 | 0.814873 | 1.23E-51 | -1.69E-46 |
| MS.gene055647 | MS.gene71609 | 0.818891 | 1.55E-52 | -1.69E-46 |
| MS.gene055722 | MS.gene71609 | 0.855439 | 6.30E-62 | -1.69E-46 |
| MS.gene05592 | MS.gene71609 | 0.898241 | 6.44E-77 | -1.69E-46 |
| MS.gene055941 | MS.gene71609 | 0.842988 | 1.85E-58 | -1.69E-46 |
| MS.gene056083 | MS.gene71609 | 0.818275 | 2.13E-52 | -1.69E-46 |
| MS.gene056374 | MS.gene71609 | 0.837284 | 5.69E-57 | -1.69E-46 |
| MS.gene056410 | MS.gene71609 | 0.80756 | 4.76E-50 | -1.69E-46 |
| MS.gene056422 | MS.gene71609 | 0.800676 | 1.29E-48 | -1.69E-46 |
| MS.gene056448 | MS.gene71609 | 0.842331 | 2.76E-58 | -1.69E-46 |
| MS.gene056487 | MS.gene71609 | 0.806804 | 6.89E-50 | -1.69E-46 |
| MS.gene056511 | MS.gene71609 | 0.833732 | 4.50E-56 | -1.69E-46 |
| MS.gene056549 | MS.gene71609 | 0.800229 | 1.59E-48 | -1.69E-46 |
| MS.gene056657 | MS.gene71609 | 0.838683 | 2.49E-57 | -1.69E-46 |
| MS.gene056692 | MS.gene71609 | 0.816685 | 4.87E-52 | -1.69E-46 |
| MS.gene056898 | MS.gene71609 | 0.815194 | 1.05E-51 | -1.69E-46 |
| MS.gene057011 | MS.gene71609 | 0.82189 | 3.17E-53 | -1.69E-46 |
| MS.gene057076 | MS.gene71609 | 0.828895 | 6.97E-55 | -1.69E-46 |
| MS.gene057123 | MS.gene71609 | 0.801945 | 7.08E-49 | -1.69E-46 |
| MS.gene057129 | MS.gene71609 | 0.855175 | 7.52E-62 | -1.69E-46 |
| MS.gene057146 | MS.gene71609 | 0.856823 | 2.48E-62 | -1.69E-46 |
| MS.gene057285 | MS.gene71609 | 0.812377 | 4.37E-51 | -1.69E-46 |
| MS.gene057331 | MS.gene71609 | 0.844566 | 6.98E-59 | -1.69E-46 |
| MS.gene057416 | MS.gene71609 | 0.88875 | 4.48E-73 | -1.69E-46 |
| MS.gene057856 | MS.gene71609 | 0.827585 | 1.44E-54 | -1.69E-46 |
| MS.gene057857 | MS.gene71609 | 0.805569 | 1.25E-49 | -1.69E-46 |
| MS.gene057926 | MS.gene71609 | 0.850786 | 1.36E-60 | -1.69E-46 |
| MS.gene058006 | MS.gene71609 | 0.829994 | 3.77E-55 | -1.69E-46 |
| MS.gene058019 | MS.gene71609 | 0.81754 | 3.13E-52 | -1.69E-46 |
| MS.gene058130 | MS.gene71609 | 0.811039 | 8.54E-51 | -1.69E-46 |
| MS.gene058323 | MS.gene71609 | 0.836681 | 8.11E-57 | -1.69E-46 |
| MS.gene058376 | MS.gene71609 | 0.800318 | 1.52E-48 | -1.69E-46 |
| MS.gene05837 | MS.gene71609 | 0.83945 | 1.57E-57 | -1.69E-46 |
| MS.gene058419 | MS.gene71609 | 0.86073 | 1.68E-63 | -1.69E-46 |
| MS.gene058544 | MS.gene71609 | 0.831814 | 1.35E-55 | -1.69E-46 |
| MS.gene058660 | MS.gene71609 | 0.805722 | 1.16E-49 | -1.69E-46 |
| MS.gene058723 | MS.gene71609 | 0.817478 | 3.23E-52 | -1.69E-46 |
| MS.gene058773 | MS.gene71609 | 0.841804 | 3.80E-58 | -1.69E-46 |
| MS.gene058838 | MS.gene71609 | 0.807726 | 4.39E-50 | -1.69E-46 |
| MS.gene05883 | MS.gene71609 | 0.805861 | 1.09E-49 | -1.69E-46 |
| MS.gene058840 | MS.gene71609 | -0.801069 | 1.07E-48 | -1.69E-46 |
| MS.gene059022 | MS.gene71609 | 0.822193 | 2.70E-53 | -1.69E-46 |
| MS.gene059023 | MS.gene71609 | 0.828722 | 7.68E-55 | -1.69E-46 |
| MS.gene059029 | MS.gene71609 | 0.844737 | 6.28E-59 | -1.69E-46 |
| MS.gene05913 | MS.gene71609 | 0.829014 | 6.52E-55 | -1.69E-46 |
| MS.gene05934 | MS.gene71609 | 0.86229 | 5.63E-64 | -1.69E-46 |
| MS.gene059536 | MS.gene71609 | 0.852942 | 3.32E-61 | -1.69E-46 |
| MS.gene059677 | MS.gene71609 | 0.832911 | 7.21E-56 | -1.69E-46 |
| MS.gene059688 | MS.gene71609 | 0.814845 | 1.25E-51 | -1.69E-46 |
| MS.gene059695 | MS.gene71609 | 0.801489 | 8.79E-49 | -1.69E-46 |
| MS.gene059711 | MS.gene71609 | 0.823453 | 1.37E-53 | -1.69E-46 |
| MS.gene059718 | MS.gene71609 | 0.854558 | 1.14E-61 | -1.69E-46 |
| MS.gene059801 | MS.gene71609 | 0.809301 | 2.02E-50 | -1.69E-46 |
| MS.gene059902 | MS.gene71609 | 0.867334 | 1.48E-65 | -1.69E-46 |
| MS.gene059960 | MS.gene71609 | 0.832124 | 1.13E-55 | -1.69E-46 |
| MS.gene060193 | MS.gene71609 | 0.808048 | 3.75E-50 | -1.69E-46 |
| MS.gene060403 | MS.gene71609 | 0.806421 | 8.30E-50 | -1.69E-46 |
| MS.gene060482 | MS.gene71609 | 0.822193 | 2.70E-53 | -1.69E-46 |
| MS.gene060762 | MS.gene71609 | 0.807311 | 5.38E-50 | -1.69E-46 |
| MS.gene06077 | MS.gene71609 | 0.801098 | 1.06E-48 | -1.69E-46 |
| MS.gene060804 | MS.gene71609 | 0.87548 | 2.98E-68 | -1.69E-46 |
| MS.gene060818 | MS.gene71609 | 0.85346 | 2.35E-61 | -1.69E-46 |
| MS.gene060835 | MS.gene71609 | 0.826038 | 3.38E-54 | -1.69E-46 |
| MS.gene060837 | MS.gene71609 | 0.847979 | 8.21E-60 | -1.69E-46 |
| MS.gene06092 | MS.gene71609 | 0.879272 | 1.43E-69 | -1.69E-46 |
| MS.gene061041 | MS.gene71609 | 0.833127 | 6.37E-56 | -1.69E-46 |
| MS.gene061134 | MS.gene71609 | 0.845853 | 3.13E-59 | -1.69E-46 |
| MS.gene061150 | MS.gene71609 | 0.823287 | 1.50E-53 | -1.69E-46 |
| MS.gene061171 | MS.gene71609 | 0.80237 | 5.79E-49 | -1.69E-46 |
| MS.gene061200 | MS.gene71609 | 0.859029 | 5.48E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene015317 | MS.gene71609 | PPI |
| MS.gene21474 | MS.gene71609 | PPI |
| MS.gene09661 | MS.gene71609 | PPI |
| MS.gene059677 | MS.gene71609 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71609.t1 | MTR_1g106190 | 97.021 | 235 | 7 | 0 | 1 | 235 | 1 | 235 | 2.04e-174 | 479 |
| MS.gene71609.t1 | MTR_3g111580 | 32.143 | 168 | 98 | 7 | 1 | 156 | 44 | 207 | 4.00e-16 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71609.t1 | AT1G04640 | 74.043 | 235 | 61 | 0 | 1 | 235 | 1 | 235 | 1.42e-132 | 373 |
| MS.gene71609.t1 | AT1G04640 | 74.043 | 235 | 61 | 0 | 1 | 235 | 1 | 235 | 1.42e-132 | 373 |
| MS.gene71609.t1 | AT1G47578 | 35.323 | 201 | 117 | 4 | 23 | 214 | 70 | 266 | 5.43e-34 | 123 |
| MS.gene71609.t1 | AT4G31050 | 35.047 | 214 | 122 | 6 | 14 | 214 | 54 | 263 | 5.95e-34 | 123 |
Find 56 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCCTTGAGGTATGGAAAA+TGG | 0.246851 | 1.4:+82143966 | MS.gene71609:CDS |
| AATGGGAGTCGTCAACTATT+TGG | 0.263377 | 1.4:+82143984 | MS.gene71609:CDS |
| CCTGGACGGAAGTGCGAGAC+TGG | 0.289747 | 1.4:+82144339 | MS.gene71609:CDS |
| ATCCTGATTTAAACTACTTT+AGG | 0.329725 | 1.4:+82144448 | MS.gene71609:CDS |
| ATTAATCCCTCAGTCAGAAT+TGG | 0.351556 | 1.4:+82144131 | MS.gene71609:CDS |
| GGAGGAGACATTACATTTCA+TGG | 0.377047 | 1.4:+82144189 | MS.gene71609:CDS |
| ATTGAACTGGCAGGTCTATA+CGG | 0.398561 | 1.4:+82144303 | MS.gene71609:CDS |
| TGCCTAAAGTAGTTTAAATC+AGG | 0.405093 | 1.4:-82144450 | None:intergenic |
| GTGTCCCGGAGCGATATAAT+AGG | 0.424169 | 1.4:-82144234 | None:intergenic |
| TTGGTTATGATAACCTGGTT+TGG | 0.424805 | 1.4:+82144592 | MS.gene71609:CDS |
| TGAGTTAACTATGATTGAAC+TGG | 0.440439 | 1.4:+82144290 | MS.gene71609:CDS |
| CTCTTGTATTATTTCTTCTG+CGG | 0.453281 | 1.4:-82144541 | None:intergenic |
| ACCCTTGAGGTATGGAAAAT+GGG | 0.459093 | 1.4:+82143967 | MS.gene71609:CDS |
| TCGCTCCGGGACACGGGACT+TGG | 0.469720 | 1.4:+82144243 | MS.gene71609:CDS |
| GGAAGTGCGAGACTGGGGTG+TGG | 0.469895 | 1.4:+82144346 | MS.gene71609:CDS |
| CTTTGCGAACCCTTGAGGTA+TGG | 0.471055 | 1.4:+82143959 | MS.gene71609:CDS |
| AGAAATAATACAAGAGCAGT+TGG | 0.475927 | 1.4:+82144548 | MS.gene71609:CDS |
| TCGAATGCAATCACTTCTCA+TGG | 0.484306 | 1.4:+82144408 | MS.gene71609:CDS |
| GAAAGAAAGATAGGTGCAAT+TGG | 0.488210 | 1.4:+82144375 | MS.gene71609:CDS |
| TATATCCTATTATATCGCTC+CGG | 0.489442 | 1.4:+82144229 | MS.gene71609:CDS |
| ACTGCAAGAAAAGCTAGTCT+TGG | 0.489810 | 1.4:+82144017 | MS.gene71609:CDS |
| TATTATATCGCTCCGGGACA+CGG | 0.491774 | 1.4:+82144236 | MS.gene71609:CDS |
| AGATAGGTGCAATTGGTGTT+CGG | 0.495508 | 1.4:+82144382 | MS.gene71609:CDS |
| CAGTCAGAATTGGAACAGAT+TGG | 0.505622 | 1.4:+82144141 | MS.gene71609:CDS |
| TACGGTGTGAAAGCTTGTCC+TGG | 0.514005 | 1.4:+82144321 | MS.gene71609:CDS |
| ATATCCTATTATATCGCTCC+GGG | 0.515465 | 1.4:+82144230 | MS.gene71609:CDS |
| TGCGAGACTGGGGTGTGGGT+TGG | 0.517715 | 1.4:+82144351 | MS.gene71609:CDS |
| CGATATAATAGGATATAAAA+CGG | 0.517995 | 1.4:-82144223 | None:intergenic |
| CCAGTCTCGCACTTCCGTCC+AGG | 0.521959 | 1.4:-82144339 | None:intergenic |
| TTGCCAACTGTGTATGTAGG+AGG | 0.525301 | 1.4:-82144087 | None:intergenic |
| CTGGACGGAAGTGCGAGACT+GGG | 0.527482 | 1.4:+82144340 | MS.gene71609:CDS |
| TAGCACCAAGTCCCGTGTCC+CGG | 0.536717 | 1.4:-82144248 | None:intergenic |
| TCTTGTATTATTTCTTCTGC+GGG | 0.538283 | 1.4:-82144540 | None:intergenic |
| TGGGTTGGAGAAAGAAAGAT+AGG | 0.538417 | 1.4:+82144366 | MS.gene71609:CDS |
| TGCAATCACTTCTCATGGGA+TGG | 0.538484 | 1.4:+82144413 | MS.gene71609:CDS |
| ATTATATCGCTCCGGGACAC+GGG | 0.540311 | 1.4:+82144237 | MS.gene71609:CDS |
| GTATTATTTCTTCTGCGGGA+AGG | 0.543520 | 1.4:-82144536 | None:intergenic |
| TTATGATAACCTGGTTTGGA+AGG | 0.544272 | 1.4:+82144596 | MS.gene71609:CDS |
| TAATAGGATATAAAACGGCT+TGG | 0.546253 | 1.4:-82144218 | None:intergenic |
| TGATAACCTGGTTTGGAAGG+AGG | 0.556596 | 1.4:+82144599 | MS.gene71609:CDS |
| TCTGTTCCAATTCTGACTGA+GGG | 0.560843 | 1.4:-82144137 | None:intergenic |
| GAAGTGCGAGACTGGGGTGT+GGG | 0.568019 | 1.4:+82144347 | MS.gene71609:CDS |
| AAGCATCCTCCTTCCAAACC+AGG | 0.571828 | 1.4:-82144605 | None:intergenic |
| GGATATAAAACGGCTTGGTG+TGG | 0.578369 | 1.4:-82144213 | None:intergenic |
| ATCTGTTCCAATTCTGACTG+AGG | 0.581218 | 1.4:-82144138 | None:intergenic |
| CACCCTCCTACATACACAGT+TGG | 0.584308 | 1.4:+82144084 | MS.gene71609:CDS |
| TTAACTATGATTGAACTGGC+AGG | 0.588243 | 1.4:+82144294 | MS.gene71609:CDS |
| TGCCAACTGTGTATGTAGGA+GGG | 0.592743 | 1.4:-82144086 | None:intergenic |
| GAGGGCTTTGCGAACCCTTG+AGG | 0.595771 | 1.4:+82143954 | MS.gene71609:CDS |
| TGGACGGAAGTGCGAGACTG+GGG | 0.596502 | 1.4:+82144341 | MS.gene71609:CDS |
| CGAATGCAATCACTTCTCAT+GGG | 0.616033 | 1.4:+82144409 | MS.gene71609:CDS |
| GTGTGAAAGCTTGTCCTGGA+CGG | 0.630714 | 1.4:+82144325 | MS.gene71609:CDS |
| TCTTTATCAGCAATTCCACA+CGG | 0.675103 | 1.4:-82144480 | None:intergenic |
| TTTAGGCACATTGTACCGTG+TGG | 0.676153 | 1.4:+82144465 | MS.gene71609:CDS |
| CTTCACTACACACAACGTGG+AGG | 0.719716 | 1.4:+82144171 | MS.gene71609:CDS |
| GAGCTTCACTACACACAACG+TGG | 0.727037 | 1.4:+82144168 | MS.gene71609:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CGATATAATAGGATATAAAA+CGG | - | chr1.4:82144226-82144245 | None:intergenic | 20.0% |
| ! | ATCCTGATTTAAACTACTTT+AGG | + | chr1.4:82144448-82144467 | MS.gene71609:CDS | 25.0% |
| !! | TCATGTTTTGCAAGAATTTT+TGG | + | chr1.4:82144573-82144592 | MS.gene71609:CDS | 25.0% |
| !!! | AATTTTTGGTTATGATAACC+TGG | + | chr1.4:82144587-82144606 | MS.gene71609:CDS | 25.0% |
| AGAAATAATACAAGAGCAGT+TGG | + | chr1.4:82144548-82144567 | MS.gene71609:CDS | 30.0% | |
| TAATAGGATATAAAACGGCT+TGG | - | chr1.4:82144221-82144240 | None:intergenic | 30.0% | |
| TATATCCTATTATATCGCTC+CGG | + | chr1.4:82144229-82144248 | MS.gene71609:CDS | 30.0% | |
| TGAGTTAACTATGATTGAAC+TGG | + | chr1.4:82144290-82144309 | MS.gene71609:CDS | 30.0% | |
| TGCCTAAAGTAGTTTAAATC+AGG | - | chr1.4:82144453-82144472 | None:intergenic | 30.0% | |
| ! | CTCTTGTATTATTTCTTCTG+CGG | - | chr1.4:82144544-82144563 | None:intergenic | 30.0% |
| ! | TCTTGTATTATTTCTTCTGC+GGG | - | chr1.4:82144543-82144562 | None:intergenic | 30.0% |
| ATATCCTATTATATCGCTCC+GGG | + | chr1.4:82144230-82144249 | MS.gene71609:CDS | 35.0% | |
| ATTAATCCCTCAGTCAGAAT+TGG | + | chr1.4:82144131-82144150 | MS.gene71609:CDS | 35.0% | |
| GAAAGAAAGATAGGTGCAAT+TGG | + | chr1.4:82144375-82144394 | MS.gene71609:CDS | 35.0% | |
| TCTTTATCAGCAATTCCACA+CGG | - | chr1.4:82144483-82144502 | None:intergenic | 35.0% | |
| TTAACTATGATTGAACTGGC+AGG | + | chr1.4:82144294-82144313 | MS.gene71609:CDS | 35.0% | |
| TTGGTTATGATAACCTGGTT+TGG | + | chr1.4:82144592-82144611 | MS.gene71609:CDS | 35.0% | |
| !! | TTATGATAACCTGGTTTGGA+AGG | + | chr1.4:82144596-82144615 | MS.gene71609:CDS | 35.0% |
| AACCCTTGAGGTATGGAAAA+TGG | + | chr1.4:82143966-82143985 | MS.gene71609:CDS | 40.0% | |
| AATGGGAGTCGTCAACTATT+TGG | + | chr1.4:82143984-82144003 | MS.gene71609:CDS | 40.0% | |
| ACCCTTGAGGTATGGAAAAT+GGG | + | chr1.4:82143967-82143986 | MS.gene71609:CDS | 40.0% | |
| ATCTGTTCCAATTCTGACTG+AGG | - | chr1.4:82144141-82144160 | None:intergenic | 40.0% | |
| ATTGAACTGGCAGGTCTATA+CGG | + | chr1.4:82144303-82144322 | MS.gene71609:CDS | 40.0% | |
| CAGTCAGAATTGGAACAGAT+TGG | + | chr1.4:82144141-82144160 | MS.gene71609:CDS | 40.0% | |
| CGAATGCAATCACTTCTCAT+GGG | + | chr1.4:82144409-82144428 | MS.gene71609:CDS | 40.0% | |
| GGAGGAGACATTACATTTCA+TGG | + | chr1.4:82144189-82144208 | MS.gene71609:CDS | 40.0% | |
| TCGAATGCAATCACTTCTCA+TGG | + | chr1.4:82144408-82144427 | MS.gene71609:CDS | 40.0% | |
| TCTGTTCCAATTCTGACTGA+GGG | - | chr1.4:82144140-82144159 | None:intergenic | 40.0% | |
| ! | ACTGCAAGAAAAGCTAGTCT+TGG | + | chr1.4:82144017-82144036 | MS.gene71609:CDS | 40.0% |
| ! | AGATAGGTGCAATTGGTGTT+CGG | + | chr1.4:82144382-82144401 | MS.gene71609:CDS | 40.0% |
| ! | CTTTTGCCAACTGTGTATGT+AGG | - | chr1.4:82144093-82144112 | None:intergenic | 40.0% |
| ! | GTATTATTTCTTCTGCGGGA+AGG | - | chr1.4:82144539-82144558 | None:intergenic | 40.0% |
| ! | TCCCATTTTCCATACCTCAA+GGG | - | chr1.4:82143971-82143990 | None:intergenic | 40.0% |
| ! | TGGGTTGGAGAAAGAAAGAT+AGG | + | chr1.4:82144366-82144385 | MS.gene71609:CDS | 40.0% |
| TATTATATCGCTCCGGGACA+CGG | + | chr1.4:82144236-82144255 | MS.gene71609:CDS | 45.0% | |
| TGCAATCACTTCTCATGGGA+TGG | + | chr1.4:82144413-82144432 | MS.gene71609:CDS | 45.0% | |
| TGCCAACTGTGTATGTAGGA+GGG | - | chr1.4:82144089-82144108 | None:intergenic | 45.0% | |
| TTGCCAACTGTGTATGTAGG+AGG | - | chr1.4:82144090-82144109 | None:intergenic | 45.0% | |
| TTTAGGCACATTGTACCGTG+TGG | + | chr1.4:82144465-82144484 | MS.gene71609:CDS | 45.0% | |
| ! | CTCCCATTTTCCATACCTCA+AGG | - | chr1.4:82143972-82143991 | None:intergenic | 45.0% |
| !! | GGATATAAAACGGCTTGGTG+TGG | - | chr1.4:82144216-82144235 | None:intergenic | 45.0% |
| !! | TGATAACCTGGTTTGGAAGG+AGG | + | chr1.4:82144599-82144618 | MS.gene71609:CDS | 45.0% |
| AAGCATCCTCCTTCCAAACC+AGG | - | chr1.4:82144608-82144627 | None:intergenic | 50.0% | |
| ATTATATCGCTCCGGGACAC+GGG | + | chr1.4:82144237-82144256 | MS.gene71609:CDS | 50.0% | |
| CACCCTCCTACATACACAGT+TGG | + | chr1.4:82144084-82144103 | MS.gene71609:CDS | 50.0% | |
| CTTCACTACACACAACGTGG+AGG | + | chr1.4:82144171-82144190 | MS.gene71609:CDS | 50.0% | |
| CTTTGCGAACCCTTGAGGTA+TGG | + | chr1.4:82143959-82143978 | MS.gene71609:CDS | 50.0% | |
| GAGCTTCACTACACACAACG+TGG | + | chr1.4:82144168-82144187 | MS.gene71609:CDS | 50.0% | |
| GTGTCCCGGAGCGATATAAT+AGG | - | chr1.4:82144237-82144256 | None:intergenic | 50.0% | |
| GTGTGAAAGCTTGTCCTGGA+CGG | + | chr1.4:82144325-82144344 | MS.gene71609:CDS | 50.0% | |
| TACGGTGTGAAAGCTTGTCC+TGG | + | chr1.4:82144321-82144340 | MS.gene71609:CDS | 50.0% | |
| GAAGTGCGAGACTGGGGTGT+GGG | + | chr1.4:82144347-82144366 | MS.gene71609:CDS | 60.0% | |
| GAGGGCTTTGCGAACCCTTG+AGG | + | chr1.4:82143954-82143973 | MS.gene71609:CDS | 60.0% | |
| TAGCACCAAGTCCCGTGTCC+CGG | - | chr1.4:82144251-82144270 | None:intergenic | 60.0% | |
| ! | CTGGACGGAAGTGCGAGACT+GGG | + | chr1.4:82144340-82144359 | MS.gene71609:CDS | 60.0% |
| ! | TGGACGGAAGTGCGAGACTG+GGG | + | chr1.4:82144341-82144360 | MS.gene71609:CDS | 60.0% |
| CCAGTCTCGCACTTCCGTCC+AGG | - | chr1.4:82144342-82144361 | None:intergenic | 65.0% | |
| TGCGAGACTGGGGTGTGGGT+TGG | + | chr1.4:82144351-82144370 | MS.gene71609:CDS | 65.0% | |
| ! | CCTGGACGGAAGTGCGAGAC+TGG | + | chr1.4:82144339-82144358 | MS.gene71609:CDS | 65.0% |
| ! | GGAAGTGCGAGACTGGGGTG+TGG | + | chr1.4:82144346-82144365 | MS.gene71609:CDS | 65.0% |
| TCGCTCCGGGACACGGGACT+TGG | + | chr1.4:82144243-82144262 | MS.gene71609:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 82143952 | 82144659 | 82143952 | ID=MS.gene71609 |
| chr1.4 | mRNA | 82143952 | 82144659 | 82143952 | ID=MS.gene71609.t1;Parent=MS.gene71609 |
| chr1.4 | exon | 82143952 | 82144659 | 82143952 | ID=MS.gene71609.t1.exon1;Parent=MS.gene71609.t1 |
| chr1.4 | CDS | 82143952 | 82144659 | 82143952 | ID=cds.MS.gene71609.t1;Parent=MS.gene71609.t1 |
| Gene Sequence |
| Protein sequence |
