AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91314


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91314.t1 MTR_1g106190 97.021 235 7 0 1 235 1 235 2.04e-174 479
MS.gene91314.t1 MTR_3g111580 32.143 168 98 7 1 156 44 207 4.00e-16 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91314.t1 AT1G04640 74.043 235 61 0 1 235 1 235 1.42e-132 373
MS.gene91314.t1 AT1G04640 74.043 235 61 0 1 235 1 235 1.42e-132 373
MS.gene91314.t1 AT1G47578 35.323 201 117 4 23 214 70 266 5.43e-34 123
MS.gene91314.t1 AT4G31050 35.047 214 122 6 14 214 54 263 5.95e-34 123

Find 56 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCCTTGAGGTATGGAAAA+TGG 0.246851 1.3:+72921239 MS.gene91314:CDS
AATGGGAGTCGTCAACTATT+TGG 0.263377 1.3:+72921257 MS.gene91314:CDS
CCTGGACGGAAGTGCGAGAC+TGG 0.289747 1.3:+72921612 MS.gene91314:CDS
ATCCTGATTTAAACTACTTT+AGG 0.329725 1.3:+72921721 MS.gene91314:CDS
ATTAATCCCTCAGTCAGAAT+TGG 0.351556 1.3:+72921404 MS.gene91314:CDS
GGAGGAGACATTACATTTCA+TGG 0.377047 1.3:+72921462 MS.gene91314:CDS
ATTGAACTGGCAGGTCTATA+CGG 0.398561 1.3:+72921576 MS.gene91314:CDS
TGCCTAAAGTAGTTTAAATC+AGG 0.405093 1.3:-72921723 None:intergenic
GTGTCCCGGAGCGATATAAT+AGG 0.424169 1.3:-72921507 None:intergenic
TTGGTTATGATAACCTGGTT+TGG 0.424805 1.3:+72921865 MS.gene91314:CDS
TGAGTTAACTATGATTGAAC+TGG 0.440439 1.3:+72921563 MS.gene91314:CDS
CTCTTGTATTATTTCTTCTG+CGG 0.453281 1.3:-72921814 None:intergenic
ACCCTTGAGGTATGGAAAAT+GGG 0.459093 1.3:+72921240 MS.gene91314:CDS
TCGCTCCGGGACACGGGACT+TGG 0.469720 1.3:+72921516 MS.gene91314:CDS
GGAAGTGCGAGACTGGGGTG+TGG 0.469895 1.3:+72921619 MS.gene91314:CDS
CTTTGCGAACCCTTGAGGTA+TGG 0.471055 1.3:+72921232 MS.gene91314:CDS
AGAAATAATACAAGAGCAGT+TGG 0.475927 1.3:+72921821 MS.gene91314:CDS
TCGAATGCAATCACTTCTCA+TGG 0.484306 1.3:+72921681 MS.gene91314:CDS
AGATAGGTGCAATCGGTGTT+CGG 0.488844 1.3:+72921655 MS.gene91314:CDS
TATATCCTATTATATCGCTC+CGG 0.489442 1.3:+72921502 MS.gene91314:CDS
ACTGCAAGAAAAGCTAGTCT+TGG 0.489810 1.3:+72921290 MS.gene91314:CDS
TATTATATCGCTCCGGGACA+CGG 0.491774 1.3:+72921509 MS.gene91314:CDS
CAGTCAGAATTGGAACAGAT+TGG 0.505622 1.3:+72921414 MS.gene91314:CDS
TACGGTGTGAAAGCTTGTCC+TGG 0.514005 1.3:+72921594 MS.gene91314:CDS
ATATCCTATTATATCGCTCC+GGG 0.515465 1.3:+72921503 MS.gene91314:CDS
TGCGAGACTGGGGTGTGGGT+TGG 0.517715 1.3:+72921624 MS.gene91314:CDS
CGATATAATAGGATATAAAA+CGG 0.517995 1.3:-72921496 None:intergenic
CCAGTCTCGCACTTCCGTCC+AGG 0.521959 1.3:-72921612 None:intergenic
TTGCCAACTGTGTATGTAGG+AGG 0.525301 1.3:-72921360 None:intergenic
CTGGACGGAAGTGCGAGACT+GGG 0.527482 1.3:+72921613 MS.gene91314:CDS
TAGCACCAAGTCCCGTGTCC+CGG 0.536717 1.3:-72921521 None:intergenic
TCTTGTATTATTTCTTCTGC+GGG 0.538283 1.3:-72921813 None:intergenic
TGGGTTGGAGAAAGAAAGAT+AGG 0.538417 1.3:+72921639 MS.gene91314:CDS
TGCAATCACTTCTCATGGGA+TGG 0.538484 1.3:+72921686 MS.gene91314:CDS
ATTATATCGCTCCGGGACAC+GGG 0.540311 1.3:+72921510 MS.gene91314:CDS
GTATTATTTCTTCTGCGGGA+AGG 0.543520 1.3:-72921809 None:intergenic
TTATGATAACCTGGTTTGGA+AGG 0.544272 1.3:+72921869 MS.gene91314:CDS
TAATAGGATATAAAACGGCT+TGG 0.546253 1.3:-72921491 None:intergenic
TGATAACCTGGTTTGGAAGG+AGG 0.556596 1.3:+72921872 MS.gene91314:CDS
TCTGTTCCAATTCTGACTGA+GGG 0.560843 1.3:-72921410 None:intergenic
GAAGTGCGAGACTGGGGTGT+GGG 0.568019 1.3:+72921620 MS.gene91314:CDS
AAGCATCCTCCTTCCAAACC+AGG 0.571828 1.3:-72921878 None:intergenic
GAAAGAAAGATAGGTGCAAT+CGG 0.577631 1.3:+72921648 MS.gene91314:CDS
GGATATAAAACGGCTTGGTG+TGG 0.578369 1.3:-72921486 None:intergenic
ATCTGTTCCAATTCTGACTG+AGG 0.581218 1.3:-72921411 None:intergenic
CACCCTCCTACATACACAGT+TGG 0.584308 1.3:+72921357 MS.gene91314:CDS
TTAACTATGATTGAACTGGC+AGG 0.588243 1.3:+72921567 MS.gene91314:CDS
TGCCAACTGTGTATGTAGGA+GGG 0.592743 1.3:-72921359 None:intergenic
GAGGGCTTTGCGAACCCTTG+AGG 0.595771 1.3:+72921227 MS.gene91314:CDS
TGGACGGAAGTGCGAGACTG+GGG 0.596502 1.3:+72921614 MS.gene91314:CDS
CGAATGCAATCACTTCTCAT+GGG 0.616033 1.3:+72921682 MS.gene91314:CDS
GTGTGAAAGCTTGTCCTGGA+CGG 0.630714 1.3:+72921598 MS.gene91314:CDS
TCTTTATCAGCAATTCCACA+CGG 0.675103 1.3:-72921753 None:intergenic
TTTAGGCACATTGTACCGTG+TGG 0.676153 1.3:+72921738 MS.gene91314:CDS
CTTCACTACACACAACGTGG+AGG 0.719716 1.3:+72921444 MS.gene91314:CDS
GAGCTTCACTACACACAACG+TGG 0.727037 1.3:+72921441 MS.gene91314:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CGATATAATAGGATATAAAA+CGG - chr1.3:72921499-72921518 None:intergenic 20.0%
! ATCCTGATTTAAACTACTTT+AGG + chr1.3:72921721-72921740 MS.gene91314:CDS 25.0%
!! TCATGTTTTGCAAGAATTTT+TGG + chr1.3:72921846-72921865 MS.gene91314:CDS 25.0%
!!! AATTTTTGGTTATGATAACC+TGG + chr1.3:72921860-72921879 MS.gene91314:CDS 25.0%
AGAAATAATACAAGAGCAGT+TGG + chr1.3:72921821-72921840 MS.gene91314:CDS 30.0%
TAATAGGATATAAAACGGCT+TGG - chr1.3:72921494-72921513 None:intergenic 30.0%
TATATCCTATTATATCGCTC+CGG + chr1.3:72921502-72921521 MS.gene91314:CDS 30.0%
TGAGTTAACTATGATTGAAC+TGG + chr1.3:72921563-72921582 MS.gene91314:CDS 30.0%
TGCCTAAAGTAGTTTAAATC+AGG - chr1.3:72921726-72921745 None:intergenic 30.0%
! CTCTTGTATTATTTCTTCTG+CGG - chr1.3:72921817-72921836 None:intergenic 30.0%
! TCTTGTATTATTTCTTCTGC+GGG - chr1.3:72921816-72921835 None:intergenic 30.0%
ATATCCTATTATATCGCTCC+GGG + chr1.3:72921503-72921522 MS.gene91314:CDS 35.0%
ATTAATCCCTCAGTCAGAAT+TGG + chr1.3:72921404-72921423 MS.gene91314:CDS 35.0%
GAAAGAAAGATAGGTGCAAT+CGG + chr1.3:72921648-72921667 MS.gene91314:CDS 35.0%
TCTTTATCAGCAATTCCACA+CGG - chr1.3:72921756-72921775 None:intergenic 35.0%
TTAACTATGATTGAACTGGC+AGG + chr1.3:72921567-72921586 MS.gene91314:CDS 35.0%
TTGGTTATGATAACCTGGTT+TGG + chr1.3:72921865-72921884 MS.gene91314:CDS 35.0%
!! TTATGATAACCTGGTTTGGA+AGG + chr1.3:72921869-72921888 MS.gene91314:CDS 35.0%
AACCCTTGAGGTATGGAAAA+TGG + chr1.3:72921239-72921258 MS.gene91314:CDS 40.0%
AATGGGAGTCGTCAACTATT+TGG + chr1.3:72921257-72921276 MS.gene91314:CDS 40.0%
ACCCTTGAGGTATGGAAAAT+GGG + chr1.3:72921240-72921259 MS.gene91314:CDS 40.0%
ATCTGTTCCAATTCTGACTG+AGG - chr1.3:72921414-72921433 None:intergenic 40.0%
ATTGAACTGGCAGGTCTATA+CGG + chr1.3:72921576-72921595 MS.gene91314:CDS 40.0%
CAGTCAGAATTGGAACAGAT+TGG + chr1.3:72921414-72921433 MS.gene91314:CDS 40.0%
CGAATGCAATCACTTCTCAT+GGG + chr1.3:72921682-72921701 MS.gene91314:CDS 40.0%
GGAGGAGACATTACATTTCA+TGG + chr1.3:72921462-72921481 MS.gene91314:CDS 40.0%
TCGAATGCAATCACTTCTCA+TGG + chr1.3:72921681-72921700 MS.gene91314:CDS 40.0%
TCTGTTCCAATTCTGACTGA+GGG - chr1.3:72921413-72921432 None:intergenic 40.0%
! ACTGCAAGAAAAGCTAGTCT+TGG + chr1.3:72921290-72921309 MS.gene91314:CDS 40.0%
! CTTTTGCCAACTGTGTATGT+AGG - chr1.3:72921366-72921385 None:intergenic 40.0%
! GTATTATTTCTTCTGCGGGA+AGG - chr1.3:72921812-72921831 None:intergenic 40.0%
! TCCCATTTTCCATACCTCAA+GGG - chr1.3:72921244-72921263 None:intergenic 40.0%
! TGGGTTGGAGAAAGAAAGAT+AGG + chr1.3:72921639-72921658 MS.gene91314:CDS 40.0%
TATTATATCGCTCCGGGACA+CGG + chr1.3:72921509-72921528 MS.gene91314:CDS 45.0%
TGCAATCACTTCTCATGGGA+TGG + chr1.3:72921686-72921705 MS.gene91314:CDS 45.0%
TGCCAACTGTGTATGTAGGA+GGG - chr1.3:72921362-72921381 None:intergenic 45.0%
TTGCCAACTGTGTATGTAGG+AGG - chr1.3:72921363-72921382 None:intergenic 45.0%
TTTAGGCACATTGTACCGTG+TGG + chr1.3:72921738-72921757 MS.gene91314:CDS 45.0%
! AGATAGGTGCAATCGGTGTT+CGG + chr1.3:72921655-72921674 MS.gene91314:CDS 45.0%
! CTCCCATTTTCCATACCTCA+AGG - chr1.3:72921245-72921264 None:intergenic 45.0%
!! GGATATAAAACGGCTTGGTG+TGG - chr1.3:72921489-72921508 None:intergenic 45.0%
!! TGATAACCTGGTTTGGAAGG+AGG + chr1.3:72921872-72921891 MS.gene91314:CDS 45.0%
AAGCATCCTCCTTCCAAACC+AGG - chr1.3:72921881-72921900 None:intergenic 50.0%
ATTATATCGCTCCGGGACAC+GGG + chr1.3:72921510-72921529 MS.gene91314:CDS 50.0%
CACCCTCCTACATACACAGT+TGG + chr1.3:72921357-72921376 MS.gene91314:CDS 50.0%
CTTCACTACACACAACGTGG+AGG + chr1.3:72921444-72921463 MS.gene91314:CDS 50.0%
CTTTGCGAACCCTTGAGGTA+TGG + chr1.3:72921232-72921251 MS.gene91314:CDS 50.0%
GAGCTTCACTACACACAACG+TGG + chr1.3:72921441-72921460 MS.gene91314:CDS 50.0%
GTGTCCCGGAGCGATATAAT+AGG - chr1.3:72921510-72921529 None:intergenic 50.0%
GTGTGAAAGCTTGTCCTGGA+CGG + chr1.3:72921598-72921617 MS.gene91314:CDS 50.0%
TACGGTGTGAAAGCTTGTCC+TGG + chr1.3:72921594-72921613 MS.gene91314:CDS 50.0%
GAAGTGCGAGACTGGGGTGT+GGG + chr1.3:72921620-72921639 MS.gene91314:CDS 60.0%
GAGGGCTTTGCGAACCCTTG+AGG + chr1.3:72921227-72921246 MS.gene91314:CDS 60.0%
TAGCACCAAGTCCCGTGTCC+CGG - chr1.3:72921524-72921543 None:intergenic 60.0%
! CTGGACGGAAGTGCGAGACT+GGG + chr1.3:72921613-72921632 MS.gene91314:CDS 60.0%
! TGGACGGAAGTGCGAGACTG+GGG + chr1.3:72921614-72921633 MS.gene91314:CDS 60.0%
CCAGTCTCGCACTTCCGTCC+AGG - chr1.3:72921615-72921634 None:intergenic 65.0%
TGCGAGACTGGGGTGTGGGT+TGG + chr1.3:72921624-72921643 MS.gene91314:CDS 65.0%
! CCTGGACGGAAGTGCGAGAC+TGG + chr1.3:72921612-72921631 MS.gene91314:CDS 65.0%
! GGAAGTGCGAGACTGGGGTG+TGG + chr1.3:72921619-72921638 MS.gene91314:CDS 65.0%
TCGCTCCGGGACACGGGACT+TGG + chr1.3:72921516-72921535 MS.gene91314:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 72921225 72921932 72921225 ID=MS.gene91314
chr1.3 mRNA 72921225 72921932 72921225 ID=MS.gene91314.t1;Parent=MS.gene91314
chr1.3 exon 72921225 72921932 72921225 ID=MS.gene91314.t1.exon1;Parent=MS.gene91314.t1
chr1.3 CDS 72921225 72921932 72921225 ID=cds.MS.gene91314.t1;Parent=MS.gene91314.t1
Gene Sequence

>MS.gene91314

ATGAGGGCTTTGCGAACCCTTGAGGTATGGAAAATGGGAGTCGTCAACTATTTGGATGCGTTAAAACTGCAAGAAAAGCTAGTCTTGGATAGAAAATCTCATAAGAGATGTGATACTCTTCTATCTTTGCAACACCCTCCTACATACACAGTTGGCAAAAGACAAACTGTTCATAATTTATTAATCCCTCAGTCAGAATTGGAACAGATTGGCGCTGAGCTTCACTACACACAACGTGGAGGAGACATTACATTTCATGGTCCACACCAAGCCGTTTTATATCCTATTATATCGCTCCGGGACACGGGACTTGGTGCTAGAAAATTTGTAGAAAAAATTGAGTTAACTATGATTGAACTGGCAGGTCTATACGGTGTGAAAGCTTGTCCTGGACGGAAGTGCGAGACTGGGGTGTGGGTTGGAGAAAGAAAGATAGGTGCAATCGGTGTTCGGATATCGAATGCAATCACTTCTCATGGGATGGCGTTTAACATTGATCCTGATTTAAACTACTTTAGGCACATTGTACCGTGTGGAATTGCTGATAAAGAAGTGACATCTTTGAGAAGAGAGACAGATTTTGACCTTCCCGCAGAAGAAATAATACAAGAGCAGTTGGTTTCATGTTTTGCAAGAATTTTTGGTTATGATAACCTGGTTTGGAAGGAGGATGCTTCTATATTATCTGATAAAAATGAAACTGAATGA

Protein sequence

>MS.gene91314.t1

MRALRTLEVWKMGVVNYLDALKLQEKLVLDRKSHKRCDTLLSLQHPPTYTVGKRQTVHNLLIPQSELEQIGAELHYTQRGGDITFHGPHQAVLYPIISLRDTGLGARKFVEKIELTMIELAGLYGVKACPGRKCETGVWVGERKIGAIGVRISNAITSHGMAFNIDPDLNYFRHIVPCGIADKEVTSLRRETDFDLPAEEIIQEQLVSCFARIFGYDNLVWKEDASILSDKNETE