Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96697.t1 | XP_013469979.1 | 97 | 235 | 7 | 0 | 1 | 235 | 1 | 235 | 2.10E-130 | 474.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96697.t1 | Q9SXP7 | 74.0 | 235 | 61 | 0 | 1 | 235 | 1 | 235 | 1.8e-101 | 370.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96697.t1 | A0A072VQE6 | 97.0 | 235 | 7 | 0 | 1 | 235 | 1 | 235 | 1.5e-130 | 474.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene96697 | 0.867592 | 1.22E-65 | -1.69E-46 |
| MS.gene049171 | MS.gene96697 | 0.801459 | 8.92E-49 | -1.69E-46 |
| MS.gene049217 | MS.gene96697 | 0.850473 | 1.66E-60 | -1.69E-46 |
| MS.gene049222 | MS.gene96697 | 0.816994 | 4.15E-52 | -1.69E-46 |
| MS.gene049236 | MS.gene96697 | -0.813423 | 2.58E-51 | -1.69E-46 |
| MS.gene049285 | MS.gene96697 | 0.818573 | 1.83E-52 | -1.69E-46 |
| MS.gene049320 | MS.gene96697 | 0.802439 | 5.61E-49 | -1.69E-46 |
| MS.gene049351 | MS.gene96697 | 0.853249 | 2.71E-61 | -1.69E-46 |
| MS.gene049356 | MS.gene96697 | 0.802696 | 4.96E-49 | -1.69E-46 |
| MS.gene049369 | MS.gene96697 | 0.801595 | 8.36E-49 | -1.69E-46 |
| MS.gene049427 | MS.gene96697 | 0.865743 | 4.73E-65 | -1.69E-46 |
| MS.gene049609 | MS.gene96697 | 0.85857 | 7.53E-63 | -1.69E-46 |
| MS.gene049614 | MS.gene96697 | 0.805304 | 1.42E-49 | -1.69E-46 |
| MS.gene049661 | MS.gene96697 | 0.848377 | 6.37E-60 | -1.69E-46 |
| MS.gene049662 | MS.gene96697 | 0.838518 | 2.74E-57 | -1.69E-46 |
| MS.gene049805 | MS.gene96697 | 0.880631 | 4.68E-70 | -1.69E-46 |
| MS.gene050076 | MS.gene96697 | 0.838519 | 2.74E-57 | -1.69E-46 |
| MS.gene050163 | MS.gene96697 | 0.801556 | 8.52E-49 | -1.69E-46 |
| MS.gene050380 | MS.gene96697 | -0.807569 | 4.74E-50 | -1.69E-46 |
| MS.gene050397 | MS.gene96697 | 0.812217 | 4.74E-51 | -1.69E-46 |
| MS.gene050432 | MS.gene96697 | 0.852252 | 5.22E-61 | -1.69E-46 |
| MS.gene050529 | MS.gene96697 | 0.804648 | 1.95E-49 | -1.69E-46 |
| MS.gene050538 | MS.gene96697 | 0.817601 | 3.03E-52 | -1.69E-46 |
| MS.gene050593 | MS.gene96697 | 0.854789 | 9.74E-62 | -1.69E-46 |
| MS.gene050625 | MS.gene96697 | 0.8001 | 1.69E-48 | -1.69E-46 |
| MS.gene050670 | MS.gene96697 | 0.861963 | 7.09E-64 | -1.69E-46 |
| MS.gene050686 | MS.gene96697 | 0.821429 | 4.06E-53 | -1.69E-46 |
| MS.gene050747 | MS.gene96697 | 0.833352 | 5.60E-56 | -1.69E-46 |
| MS.gene050767 | MS.gene96697 | 0.817762 | 2.79E-52 | -1.69E-46 |
| MS.gene050770 | MS.gene96697 | 0.823971 | 1.04E-53 | -1.69E-46 |
| MS.gene050772 | MS.gene96697 | 0.853096 | 2.99E-61 | -1.69E-46 |
| MS.gene050950 | MS.gene96697 | 0.824081 | 9.79E-54 | -1.69E-46 |
| MS.gene05101 | MS.gene96697 | 0.844313 | 8.17E-59 | -1.69E-46 |
| MS.gene05105 | MS.gene96697 | 0.867979 | 9.18E-66 | -1.69E-46 |
| MS.gene051113 | MS.gene96697 | -0.811421 | 7.06E-51 | -1.69E-46 |
| MS.gene051387 | MS.gene96697 | 0.882428 | 1.05E-70 | -1.69E-46 |
| MS.gene051582 | MS.gene96697 | 0.815259 | 1.01E-51 | -1.69E-46 |
| MS.gene051666 | MS.gene96697 | 0.860442 | 2.06E-63 | -1.69E-46 |
| MS.gene051692 | MS.gene96697 | 0.848968 | 4.37E-60 | -1.69E-46 |
| MS.gene051694 | MS.gene96697 | 0.84384 | 1.09E-58 | -1.69E-46 |
| MS.gene051858 | MS.gene96697 | 0.852542 | 4.31E-61 | -1.69E-46 |
| MS.gene051877 | MS.gene96697 | 0.846301 | 2.37E-59 | -1.69E-46 |
| MS.gene052078 | MS.gene96697 | 0.812717 | 3.68E-51 | -1.69E-46 |
| MS.gene052188 | MS.gene96697 | 0.820446 | 6.83E-53 | -1.69E-46 |
| MS.gene052253 | MS.gene96697 | 0.817109 | 3.91E-52 | -1.69E-46 |
| MS.gene052271 | MS.gene96697 | 0.82742 | 1.58E-54 | -1.69E-46 |
| MS.gene052290 | MS.gene96697 | 0.8102 | 1.30E-50 | -1.69E-46 |
| MS.gene052310 | MS.gene96697 | 0.852179 | 5.47E-61 | -1.69E-46 |
| MS.gene052322 | MS.gene96697 | 0.844741 | 6.26E-59 | -1.69E-46 |
| MS.gene052326 | MS.gene96697 | 0.813456 | 2.54E-51 | -1.69E-46 |
| MS.gene052379 | MS.gene96697 | 0.868234 | 7.59E-66 | -1.69E-46 |
| MS.gene052421 | MS.gene96697 | 0.875262 | 3.54E-68 | -1.69E-46 |
| MS.gene052481 | MS.gene96697 | 0.853752 | 1.94E-61 | -1.69E-46 |
| MS.gene052484 | MS.gene96697 | 0.86491 | 8.65E-65 | -1.69E-46 |
| MS.gene052485 | MS.gene96697 | 0.859916 | 2.97E-63 | -1.69E-46 |
| MS.gene052501 | MS.gene96697 | 0.823235 | 1.54E-53 | -1.69E-46 |
| MS.gene052503 | MS.gene96697 | 0.801513 | 8.69E-49 | -1.69E-46 |
| MS.gene052505 | MS.gene96697 | 0.827657 | 1.39E-54 | -1.69E-46 |
| MS.gene052615 | MS.gene96697 | 0.86327 | 2.81E-64 | -1.69E-46 |
| MS.gene052982 | MS.gene96697 | 0.82829 | 9.76E-55 | -1.69E-46 |
| MS.gene053009 | MS.gene96697 | 0.802891 | 4.52E-49 | -1.69E-46 |
| MS.gene053103 | MS.gene96697 | 0.863835 | 1.87E-64 | -1.69E-46 |
| MS.gene053104 | MS.gene96697 | 0.852539 | 4.32E-61 | -1.69E-46 |
| MS.gene053107 | MS.gene96697 | 0.802611 | 5.17E-49 | -1.69E-46 |
| MS.gene053207 | MS.gene96697 | 0.841908 | 3.57E-58 | -1.69E-46 |
| MS.gene053209 | MS.gene96697 | 0.819871 | 9.25E-53 | -1.69E-46 |
| MS.gene053213 | MS.gene96697 | 0.84793 | 8.47E-60 | -1.69E-46 |
| MS.gene053286 | MS.gene96697 | 0.835316 | 1.80E-56 | -1.69E-46 |
| MS.gene053322 | MS.gene96697 | 0.879461 | 1.22E-69 | -1.69E-46 |
| MS.gene053449 | MS.gene96697 | 0.819465 | 1.15E-52 | -1.69E-46 |
| MS.gene053574 | MS.gene96697 | 0.834591 | 2.74E-56 | -1.69E-46 |
| MS.gene053632 | MS.gene96697 | 0.848067 | 7.76E-60 | -1.69E-46 |
| MS.gene053758 | MS.gene96697 | 0.816246 | 6.11E-52 | -1.69E-46 |
| MS.gene053813 | MS.gene96697 | 0.841 | 6.19E-58 | -1.69E-46 |
| MS.gene053824 | MS.gene96697 | 0.801325 | 9.50E-49 | -1.69E-46 |
| MS.gene053915 | MS.gene96697 | 0.824884 | 6.34E-54 | -1.69E-46 |
| MS.gene053916 | MS.gene96697 | 0.80808 | 3.69E-50 | -1.69E-46 |
| MS.gene054003 | MS.gene96697 | 0.800743 | 1.25E-48 | -1.69E-46 |
| MS.gene054376 | MS.gene96697 | 0.81385 | 2.08E-51 | -1.69E-46 |
| MS.gene054408 | MS.gene96697 | 0.804005 | 2.66E-49 | -1.69E-46 |
| MS.gene054595 | MS.gene96697 | 0.813098 | 3.04E-51 | -1.69E-46 |
| MS.gene054600 | MS.gene96697 | 0.80999 | 1.44E-50 | -1.69E-46 |
| MS.gene054642 | MS.gene96697 | 0.839222 | 1.80E-57 | -1.69E-46 |
| MS.gene054802 | MS.gene96697 | 0.833171 | 6.21E-56 | -1.69E-46 |
| MS.gene054803 | MS.gene96697 | 0.830687 | 2.55E-55 | -1.69E-46 |
| MS.gene054804 | MS.gene96697 | 0.833239 | 5.98E-56 | -1.69E-46 |
| MS.gene054927 | MS.gene96697 | 0.82901 | 6.54E-55 | -1.69E-46 |
| MS.gene05505 | MS.gene96697 | 0.880006 | 7.83E-70 | -1.69E-46 |
| MS.gene055116 | MS.gene96697 | 0.826678 | 2.38E-54 | -1.69E-46 |
| MS.gene055206 | MS.gene96697 | 0.871367 | 7.21E-67 | -1.69E-46 |
| MS.gene055277 | MS.gene96697 | 0.850825 | 1.32E-60 | -1.69E-46 |
| MS.gene055289 | MS.gene96697 | 0.825917 | 3.61E-54 | -1.69E-46 |
| MS.gene055362 | MS.gene96697 | 0.803847 | 2.87E-49 | -1.69E-46 |
| MS.gene055397 | MS.gene96697 | 0.800495 | 1.40E-48 | -1.69E-46 |
| MS.gene055412 | MS.gene96697 | 0.847819 | 9.08E-60 | -1.69E-46 |
| MS.gene055428 | MS.gene96697 | 0.812268 | 4.62E-51 | -1.69E-46 |
| MS.gene055486 | MS.gene96697 | 0.848904 | 4.55E-60 | -1.69E-46 |
| MS.gene055499 | MS.gene96697 | 0.847193 | 1.35E-59 | -1.69E-46 |
| MS.gene055502 | MS.gene96697 | 0.826617 | 2.46E-54 | -1.69E-46 |
| MS.gene055577 | MS.gene96697 | 0.802413 | 5.68E-49 | -1.69E-46 |
| MS.gene055636 | MS.gene96697 | 0.800265 | 1.56E-48 | -1.69E-46 |
| MS.gene055647 | MS.gene96697 | 0.847835 | 8.99E-60 | -1.69E-46 |
| MS.gene055722 | MS.gene96697 | 0.851035 | 1.15E-60 | -1.69E-46 |
| MS.gene055911 | MS.gene96697 | 0.801949 | 7.07E-49 | -1.69E-46 |
| MS.gene05592 | MS.gene96697 | 0.868453 | 6.46E-66 | -1.69E-46 |
| MS.gene055941 | MS.gene96697 | 0.838393 | 2.95E-57 | -1.69E-46 |
| MS.gene056083 | MS.gene96697 | 0.815388 | 9.49E-52 | -1.69E-46 |
| MS.gene056191 | MS.gene96697 | 0.83176 | 1.39E-55 | -1.69E-46 |
| MS.gene056374 | MS.gene96697 | 0.833658 | 4.70E-56 | -1.69E-46 |
| MS.gene056407 | MS.gene96697 | 0.837067 | 6.47E-57 | -1.69E-46 |
| MS.gene056410 | MS.gene96697 | 0.83003 | 3.69E-55 | -1.69E-46 |
| MS.gene056422 | MS.gene96697 | 0.814767 | 1.30E-51 | -1.69E-46 |
| MS.gene056448 | MS.gene96697 | 0.83653 | 8.87E-57 | -1.69E-46 |
| MS.gene056452 | MS.gene96697 | 0.811578 | 6.53E-51 | -1.69E-46 |
| MS.gene056511 | MS.gene96697 | 0.851485 | 8.61E-61 | -1.69E-46 |
| MS.gene056549 | MS.gene96697 | 0.803268 | 3.78E-49 | -1.69E-46 |
| MS.gene056609 | MS.gene96697 | 0.802529 | 5.37E-49 | -1.69E-46 |
| MS.gene056657 | MS.gene96697 | 0.808855 | 2.52E-50 | -1.69E-46 |
| MS.gene056796 | MS.gene96697 | 0.809122 | 2.21E-50 | -1.69E-46 |
| MS.gene056942 | MS.gene96697 | 0.818242 | 2.17E-52 | -1.69E-46 |
| MS.gene057011 | MS.gene96697 | 0.878264 | 3.23E-69 | -1.69E-46 |
| MS.gene057048 | MS.gene96697 | 0.801273 | 9.73E-49 | -1.69E-46 |
| MS.gene057076 | MS.gene96697 | 0.83176 | 1.39E-55 | -1.69E-46 |
| MS.gene057123 | MS.gene96697 | 0.842903 | 1.95E-58 | -1.69E-46 |
| MS.gene057124 | MS.gene96697 | 0.824701 | 7.00E-54 | -1.69E-46 |
| MS.gene057129 | MS.gene96697 | 0.86591 | 4.19E-65 | -1.69E-46 |
| MS.gene057146 | MS.gene96697 | 0.896963 | 2.23E-76 | -1.69E-46 |
| MS.gene057285 | MS.gene96697 | 0.833064 | 6.61E-56 | -1.69E-46 |
| MS.gene057331 | MS.gene96697 | 0.820693 | 5.99E-53 | -1.69E-46 |
| MS.gene05733 | MS.gene96697 | 0.803717 | 3.05E-49 | -1.69E-46 |
| MS.gene057416 | MS.gene96697 | 0.876453 | 1.38E-68 | -1.69E-46 |
| MS.gene057478 | MS.gene96697 | -0.811796 | 5.85E-51 | -1.69E-46 |
| MS.gene057749 | MS.gene96697 | 0.825749 | 3.96E-54 | -1.69E-46 |
| MS.gene057823 | MS.gene96697 | 0.836589 | 8.56E-57 | -1.69E-46 |
| MS.gene057845 | MS.gene96697 | 0.817225 | 3.68E-52 | -1.69E-46 |
| MS.gene057856 | MS.gene96697 | 0.830602 | 2.68E-55 | -1.69E-46 |
| MS.gene057926 | MS.gene96697 | 0.810103 | 1.36E-50 | -1.69E-46 |
| MS.gene058006 | MS.gene96697 | 0.845465 | 3.99E-59 | -1.69E-46 |
| MS.gene058007 | MS.gene96697 | 0.809809 | 1.58E-50 | -1.69E-46 |
| MS.gene058019 | MS.gene96697 | 0.845789 | 3.26E-59 | -1.69E-46 |
| MS.gene05811 | MS.gene96697 | 0.801342 | 9.42E-49 | -1.69E-46 |
| MS.gene058130 | MS.gene96697 | 0.820938 | 5.26E-53 | -1.69E-46 |
| MS.gene058233 | MS.gene96697 | 0.826525 | 2.59E-54 | -1.69E-46 |
| MS.gene058293 | MS.gene96697 | 0.830685 | 2.55E-55 | -1.69E-46 |
| MS.gene058323 | MS.gene96697 | 0.832202 | 1.08E-55 | -1.69E-46 |
| MS.gene058376 | MS.gene96697 | 0.836516 | 8.94E-57 | -1.69E-46 |
| MS.gene05837 | MS.gene96697 | 0.809572 | 1.77E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene96697 | 0.835706 | 1.44E-56 | -1.69E-46 |
| MS.gene058544 | MS.gene96697 | 0.818482 | 1.92E-52 | -1.69E-46 |
| MS.gene058660 | MS.gene96697 | 0.873787 | 1.12E-67 | -1.69E-46 |
| MS.gene058787 | MS.gene96697 | 0.801042 | 1.09E-48 | -1.69E-46 |
| MS.gene058838 | MS.gene96697 | 0.800818 | 1.21E-48 | -1.69E-46 |
| MS.gene05883 | MS.gene96697 | 0.842498 | 2.49E-58 | -1.69E-46 |
| MS.gene058928 | MS.gene96697 | 0.824281 | 8.79E-54 | -1.69E-46 |
| MS.gene059023 | MS.gene96697 | 0.800815 | 1.21E-48 | -1.69E-46 |
| MS.gene059029 | MS.gene96697 | 0.829174 | 5.97E-55 | -1.69E-46 |
| MS.gene05907 | MS.gene96697 | 0.818156 | 2.27E-52 | -1.69E-46 |
| MS.gene059141 | MS.gene96697 | 0.828164 | 1.05E-54 | -1.69E-46 |
| MS.gene05934 | MS.gene96697 | 0.846218 | 2.49E-59 | -1.69E-46 |
| MS.gene059366 | MS.gene96697 | 0.801019 | 1.10E-48 | -1.69E-46 |
| MS.gene059368 | MS.gene96697 | 0.809485 | 1.85E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene96697 | 0.805204 | 1.49E-49 | -1.69E-46 |
| MS.gene059455 | MS.gene96697 | 0.827639 | 1.40E-54 | -1.69E-46 |
| MS.gene059536 | MS.gene96697 | 0.881572 | 2.15E-70 | -1.69E-46 |
| MS.gene059597 | MS.gene96697 | 0.815012 | 1.15E-51 | -1.69E-46 |
| MS.gene059676 | MS.gene96697 | 0.848462 | 6.04E-60 | -1.69E-46 |
| MS.gene059688 | MS.gene96697 | 0.8762 | 1.69E-68 | -1.69E-46 |
| MS.gene059692 | MS.gene96697 | 0.856272 | 3.60E-62 | -1.69E-46 |
| MS.gene059695 | MS.gene96697 | 0.857722 | 1.34E-62 | -1.69E-46 |
| MS.gene059711 | MS.gene96697 | 0.834075 | 3.70E-56 | -1.69E-46 |
| MS.gene059718 | MS.gene96697 | 0.84029 | 9.51E-58 | -1.69E-46 |
| MS.gene059801 | MS.gene96697 | 0.808999 | 2.35E-50 | -1.69E-46 |
| MS.gene059902 | MS.gene96697 | 0.853689 | 2.02E-61 | -1.69E-46 |
| MS.gene059960 | MS.gene96697 | 0.856871 | 2.40E-62 | -1.69E-46 |
| MS.gene060403 | MS.gene96697 | 0.854587 | 1.11E-61 | -1.69E-46 |
| MS.gene060482 | MS.gene96697 | 0.813539 | 2.43E-51 | -1.69E-46 |
| MS.gene060804 | MS.gene96697 | 0.880007 | 7.83E-70 | -1.69E-46 |
| MS.gene060818 | MS.gene96697 | 0.86959 | 2.76E-66 | -1.69E-46 |
| MS.gene060835 | MS.gene96697 | 0.824902 | 6.28E-54 | -1.69E-46 |
| MS.gene060837 | MS.gene96697 | 0.861794 | 7.99E-64 | -1.69E-46 |
| MS.gene06092 | MS.gene96697 | 0.861596 | 9.18E-64 | -1.69E-46 |
| MS.gene061041 | MS.gene96697 | 0.831359 | 1.75E-55 | -1.69E-46 |
| MS.gene061134 | MS.gene96697 | 0.823347 | 1.45E-53 | -1.69E-46 |
| MS.gene061150 | MS.gene96697 | 0.821697 | 3.52E-53 | -1.69E-46 |
| MS.gene061200 | MS.gene96697 | 0.87165 | 5.81E-67 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene059677 | MS.gene96697 | PPI |
| MS.gene015317 | MS.gene96697 | PPI |
| MS.gene96697 | MS.gene21474 | PPI |
| MS.gene09661 | MS.gene96697 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96697.t1 | MTR_1g106190 | 97.021 | 235 | 7 | 0 | 1 | 235 | 1 | 235 | 2.04e-174 | 479 |
| MS.gene96697.t1 | MTR_3g111580 | 32.143 | 168 | 98 | 7 | 1 | 156 | 44 | 207 | 4.00e-16 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96697.t1 | AT1G04640 | 74.043 | 235 | 61 | 0 | 1 | 235 | 1 | 235 | 1.42e-132 | 373 |
| MS.gene96697.t1 | AT1G04640 | 74.043 | 235 | 61 | 0 | 1 | 235 | 1 | 235 | 1.42e-132 | 373 |
| MS.gene96697.t1 | AT1G47578 | 35.323 | 201 | 117 | 4 | 23 | 214 | 70 | 266 | 5.43e-34 | 123 |
| MS.gene96697.t1 | AT4G31050 | 35.047 | 214 | 122 | 6 | 14 | 214 | 54 | 263 | 5.95e-34 | 123 |
Find 56 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCCTTGAGGTATGGAAAA+TGG | 0.246851 | 1.1:+76393762 | MS.gene96697:CDS |
| AATGGGAGTCGTCAACTATT+TGG | 0.263377 | 1.1:+76393780 | MS.gene96697:CDS |
| CCTGGACGGAAGTGCGAGAC+TGG | 0.289747 | 1.1:+76394135 | MS.gene96697:CDS |
| ATCCTGATTTAAACTACTTT+AGG | 0.329725 | 1.1:+76394244 | MS.gene96697:CDS |
| ATTAATCCCTCAGTCAGAAT+TGG | 0.351556 | 1.1:+76393927 | MS.gene96697:CDS |
| GGAGGAGACATTACATTTCA+TGG | 0.377047 | 1.1:+76393985 | MS.gene96697:CDS |
| ATTGAACTGGCAGGTCTATA+CGG | 0.398561 | 1.1:+76394099 | MS.gene96697:CDS |
| TGCCTAAAGTAGTTTAAATC+AGG | 0.405093 | 1.1:-76394246 | None:intergenic |
| GTGTCCCGGAGCGATATAAT+AGG | 0.424169 | 1.1:-76394030 | None:intergenic |
| TTGGTTATGATAACCTGGTT+TGG | 0.424805 | 1.1:+76394388 | MS.gene96697:CDS |
| TGAGTTAACTATGATTGAAC+TGG | 0.440439 | 1.1:+76394086 | MS.gene96697:CDS |
| CTCTTGTATTATTTCTTCTG+CGG | 0.453281 | 1.1:-76394337 | None:intergenic |
| ACCCTTGAGGTATGGAAAAT+GGG | 0.459093 | 1.1:+76393763 | MS.gene96697:CDS |
| TCGCTCCGGGACACGGGACT+TGG | 0.469720 | 1.1:+76394039 | MS.gene96697:CDS |
| GGAAGTGCGAGACTGGGGTG+TGG | 0.469895 | 1.1:+76394142 | MS.gene96697:CDS |
| CTTTGCGAACCCTTGAGGTA+TGG | 0.471055 | 1.1:+76393755 | MS.gene96697:CDS |
| AGAAATAATACAAGAGCAGT+TGG | 0.475927 | 1.1:+76394344 | MS.gene96697:CDS |
| TCGAATGCAATCACTTCTCA+TGG | 0.484306 | 1.1:+76394204 | MS.gene96697:CDS |
| AGATAGGTGCAATCGGTGTT+CGG | 0.488844 | 1.1:+76394178 | MS.gene96697:CDS |
| TATATCCTATTATATCGCTC+CGG | 0.489442 | 1.1:+76394025 | MS.gene96697:CDS |
| ACTGCAAGAAAAGCTAGTCT+TGG | 0.489810 | 1.1:+76393813 | MS.gene96697:CDS |
| TATTATATCGCTCCGGGACA+CGG | 0.491774 | 1.1:+76394032 | MS.gene96697:CDS |
| CAGTCAGAATTGGAACAGAT+TGG | 0.505622 | 1.1:+76393937 | MS.gene96697:CDS |
| TACGGTGTGAAAGCTTGTCC+TGG | 0.514005 | 1.1:+76394117 | MS.gene96697:CDS |
| ATATCCTATTATATCGCTCC+GGG | 0.515465 | 1.1:+76394026 | MS.gene96697:CDS |
| TGCGAGACTGGGGTGTGGGT+TGG | 0.517715 | 1.1:+76394147 | MS.gene96697:CDS |
| CGATATAATAGGATATAAAA+CGG | 0.517995 | 1.1:-76394019 | None:intergenic |
| CCAGTCTCGCACTTCCGTCC+AGG | 0.521959 | 1.1:-76394135 | None:intergenic |
| TTGCCAACTGTGTATGTAGG+AGG | 0.525301 | 1.1:-76393883 | None:intergenic |
| CTGGACGGAAGTGCGAGACT+GGG | 0.527482 | 1.1:+76394136 | MS.gene96697:CDS |
| TAGCACCAAGTCCCGTGTCC+CGG | 0.536717 | 1.1:-76394044 | None:intergenic |
| TCTTGTATTATTTCTTCTGC+GGG | 0.538283 | 1.1:-76394336 | None:intergenic |
| TGGGTTGGAGAAAGAAAGAT+AGG | 0.538417 | 1.1:+76394162 | MS.gene96697:CDS |
| TGCAATCACTTCTCATGGGA+TGG | 0.538484 | 1.1:+76394209 | MS.gene96697:CDS |
| ATTATATCGCTCCGGGACAC+GGG | 0.540311 | 1.1:+76394033 | MS.gene96697:CDS |
| GTATTATTTCTTCTGCGGGA+AGG | 0.543520 | 1.1:-76394332 | None:intergenic |
| TTATGATAACCTGGTTTGGA+AGG | 0.544272 | 1.1:+76394392 | MS.gene96697:CDS |
| TAATAGGATATAAAACGGCT+TGG | 0.546253 | 1.1:-76394014 | None:intergenic |
| TGATAACCTGGTTTGGAAGG+AGG | 0.556596 | 1.1:+76394395 | MS.gene96697:CDS |
| TCTGTTCCAATTCTGACTGA+GGG | 0.560843 | 1.1:-76393933 | None:intergenic |
| GAAGTGCGAGACTGGGGTGT+GGG | 0.568019 | 1.1:+76394143 | MS.gene96697:CDS |
| AAGCATCCTCCTTCCAAACC+AGG | 0.571828 | 1.1:-76394401 | None:intergenic |
| GAAAGAAAGATAGGTGCAAT+CGG | 0.577631 | 1.1:+76394171 | MS.gene96697:CDS |
| GGATATAAAACGGCTTGGTG+TGG | 0.578369 | 1.1:-76394009 | None:intergenic |
| ATCTGTTCCAATTCTGACTG+AGG | 0.581218 | 1.1:-76393934 | None:intergenic |
| CACCCTCCTACATACACAGT+TGG | 0.584308 | 1.1:+76393880 | MS.gene96697:CDS |
| TTAACTATGATTGAACTGGC+AGG | 0.588243 | 1.1:+76394090 | MS.gene96697:CDS |
| TGCCAACTGTGTATGTAGGA+GGG | 0.592743 | 1.1:-76393882 | None:intergenic |
| GAGGGCTTTGCGAACCCTTG+AGG | 0.595771 | 1.1:+76393750 | MS.gene96697:CDS |
| TGGACGGAAGTGCGAGACTG+GGG | 0.596502 | 1.1:+76394137 | MS.gene96697:CDS |
| CGAATGCAATCACTTCTCAT+GGG | 0.616033 | 1.1:+76394205 | MS.gene96697:CDS |
| GTGTGAAAGCTTGTCCTGGA+CGG | 0.630714 | 1.1:+76394121 | MS.gene96697:CDS |
| TCTTTATCAGCAATTCCACA+CGG | 0.675103 | 1.1:-76394276 | None:intergenic |
| TTTAGGCACATTGTACCGTG+TGG | 0.676153 | 1.1:+76394261 | MS.gene96697:CDS |
| CTTCACTACACACAACGTGG+AGG | 0.719716 | 1.1:+76393967 | MS.gene96697:CDS |
| GAGCTTCACTACACACAACG+TGG | 0.727037 | 1.1:+76393964 | MS.gene96697:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CGATATAATAGGATATAAAA+CGG | - | chr1.1:76394022-76394041 | None:intergenic | 20.0% |
| ! | ATCCTGATTTAAACTACTTT+AGG | + | chr1.1:76394244-76394263 | MS.gene96697:CDS | 25.0% |
| !! | TCATGTTTTGCAAGAATTTT+TGG | + | chr1.1:76394369-76394388 | MS.gene96697:CDS | 25.0% |
| !!! | AATTTTTGGTTATGATAACC+TGG | + | chr1.1:76394383-76394402 | MS.gene96697:CDS | 25.0% |
| AGAAATAATACAAGAGCAGT+TGG | + | chr1.1:76394344-76394363 | MS.gene96697:CDS | 30.0% | |
| TAATAGGATATAAAACGGCT+TGG | - | chr1.1:76394017-76394036 | None:intergenic | 30.0% | |
| TATATCCTATTATATCGCTC+CGG | + | chr1.1:76394025-76394044 | MS.gene96697:CDS | 30.0% | |
| TGAGTTAACTATGATTGAAC+TGG | + | chr1.1:76394086-76394105 | MS.gene96697:CDS | 30.0% | |
| TGCCTAAAGTAGTTTAAATC+AGG | - | chr1.1:76394249-76394268 | None:intergenic | 30.0% | |
| ! | CTCTTGTATTATTTCTTCTG+CGG | - | chr1.1:76394340-76394359 | None:intergenic | 30.0% |
| ! | TCTTGTATTATTTCTTCTGC+GGG | - | chr1.1:76394339-76394358 | None:intergenic | 30.0% |
| ATATCCTATTATATCGCTCC+GGG | + | chr1.1:76394026-76394045 | MS.gene96697:CDS | 35.0% | |
| ATTAATCCCTCAGTCAGAAT+TGG | + | chr1.1:76393927-76393946 | MS.gene96697:CDS | 35.0% | |
| GAAAGAAAGATAGGTGCAAT+CGG | + | chr1.1:76394171-76394190 | MS.gene96697:CDS | 35.0% | |
| TCTTTATCAGCAATTCCACA+CGG | - | chr1.1:76394279-76394298 | None:intergenic | 35.0% | |
| TTAACTATGATTGAACTGGC+AGG | + | chr1.1:76394090-76394109 | MS.gene96697:CDS | 35.0% | |
| TTGGTTATGATAACCTGGTT+TGG | + | chr1.1:76394388-76394407 | MS.gene96697:CDS | 35.0% | |
| !! | TTATGATAACCTGGTTTGGA+AGG | + | chr1.1:76394392-76394411 | MS.gene96697:CDS | 35.0% |
| AACCCTTGAGGTATGGAAAA+TGG | + | chr1.1:76393762-76393781 | MS.gene96697:CDS | 40.0% | |
| AATGGGAGTCGTCAACTATT+TGG | + | chr1.1:76393780-76393799 | MS.gene96697:CDS | 40.0% | |
| ACCCTTGAGGTATGGAAAAT+GGG | + | chr1.1:76393763-76393782 | MS.gene96697:CDS | 40.0% | |
| ATCTGTTCCAATTCTGACTG+AGG | - | chr1.1:76393937-76393956 | None:intergenic | 40.0% | |
| ATTGAACTGGCAGGTCTATA+CGG | + | chr1.1:76394099-76394118 | MS.gene96697:CDS | 40.0% | |
| CAGTCAGAATTGGAACAGAT+TGG | + | chr1.1:76393937-76393956 | MS.gene96697:CDS | 40.0% | |
| CGAATGCAATCACTTCTCAT+GGG | + | chr1.1:76394205-76394224 | MS.gene96697:CDS | 40.0% | |
| GGAGGAGACATTACATTTCA+TGG | + | chr1.1:76393985-76394004 | MS.gene96697:CDS | 40.0% | |
| TCGAATGCAATCACTTCTCA+TGG | + | chr1.1:76394204-76394223 | MS.gene96697:CDS | 40.0% | |
| TCTGTTCCAATTCTGACTGA+GGG | - | chr1.1:76393936-76393955 | None:intergenic | 40.0% | |
| ! | ACTGCAAGAAAAGCTAGTCT+TGG | + | chr1.1:76393813-76393832 | MS.gene96697:CDS | 40.0% |
| ! | CTTTTGCCAACTGTGTATGT+AGG | - | chr1.1:76393889-76393908 | None:intergenic | 40.0% |
| ! | GTATTATTTCTTCTGCGGGA+AGG | - | chr1.1:76394335-76394354 | None:intergenic | 40.0% |
| ! | TCCCATTTTCCATACCTCAA+GGG | - | chr1.1:76393767-76393786 | None:intergenic | 40.0% |
| ! | TGGGTTGGAGAAAGAAAGAT+AGG | + | chr1.1:76394162-76394181 | MS.gene96697:CDS | 40.0% |
| TATTATATCGCTCCGGGACA+CGG | + | chr1.1:76394032-76394051 | MS.gene96697:CDS | 45.0% | |
| TGCAATCACTTCTCATGGGA+TGG | + | chr1.1:76394209-76394228 | MS.gene96697:CDS | 45.0% | |
| TGCCAACTGTGTATGTAGGA+GGG | - | chr1.1:76393885-76393904 | None:intergenic | 45.0% | |
| TTGCCAACTGTGTATGTAGG+AGG | - | chr1.1:76393886-76393905 | None:intergenic | 45.0% | |
| TTTAGGCACATTGTACCGTG+TGG | + | chr1.1:76394261-76394280 | MS.gene96697:CDS | 45.0% | |
| ! | AGATAGGTGCAATCGGTGTT+CGG | + | chr1.1:76394178-76394197 | MS.gene96697:CDS | 45.0% |
| ! | CTCCCATTTTCCATACCTCA+AGG | - | chr1.1:76393768-76393787 | None:intergenic | 45.0% |
| !! | GGATATAAAACGGCTTGGTG+TGG | - | chr1.1:76394012-76394031 | None:intergenic | 45.0% |
| !! | TGATAACCTGGTTTGGAAGG+AGG | + | chr1.1:76394395-76394414 | MS.gene96697:CDS | 45.0% |
| AAGCATCCTCCTTCCAAACC+AGG | - | chr1.1:76394404-76394423 | None:intergenic | 50.0% | |
| ATTATATCGCTCCGGGACAC+GGG | + | chr1.1:76394033-76394052 | MS.gene96697:CDS | 50.0% | |
| CACCCTCCTACATACACAGT+TGG | + | chr1.1:76393880-76393899 | MS.gene96697:CDS | 50.0% | |
| CTTCACTACACACAACGTGG+AGG | + | chr1.1:76393967-76393986 | MS.gene96697:CDS | 50.0% | |
| CTTTGCGAACCCTTGAGGTA+TGG | + | chr1.1:76393755-76393774 | MS.gene96697:CDS | 50.0% | |
| GAGCTTCACTACACACAACG+TGG | + | chr1.1:76393964-76393983 | MS.gene96697:CDS | 50.0% | |
| GTGTCCCGGAGCGATATAAT+AGG | - | chr1.1:76394033-76394052 | None:intergenic | 50.0% | |
| GTGTGAAAGCTTGTCCTGGA+CGG | + | chr1.1:76394121-76394140 | MS.gene96697:CDS | 50.0% | |
| TACGGTGTGAAAGCTTGTCC+TGG | + | chr1.1:76394117-76394136 | MS.gene96697:CDS | 50.0% | |
| GAAGTGCGAGACTGGGGTGT+GGG | + | chr1.1:76394143-76394162 | MS.gene96697:CDS | 60.0% | |
| GAGGGCTTTGCGAACCCTTG+AGG | + | chr1.1:76393750-76393769 | MS.gene96697:CDS | 60.0% | |
| TAGCACCAAGTCCCGTGTCC+CGG | - | chr1.1:76394047-76394066 | None:intergenic | 60.0% | |
| ! | CTGGACGGAAGTGCGAGACT+GGG | + | chr1.1:76394136-76394155 | MS.gene96697:CDS | 60.0% |
| ! | TGGACGGAAGTGCGAGACTG+GGG | + | chr1.1:76394137-76394156 | MS.gene96697:CDS | 60.0% |
| CCAGTCTCGCACTTCCGTCC+AGG | - | chr1.1:76394138-76394157 | None:intergenic | 65.0% | |
| TGCGAGACTGGGGTGTGGGT+TGG | + | chr1.1:76394147-76394166 | MS.gene96697:CDS | 65.0% | |
| ! | CCTGGACGGAAGTGCGAGAC+TGG | + | chr1.1:76394135-76394154 | MS.gene96697:CDS | 65.0% |
| ! | GGAAGTGCGAGACTGGGGTG+TGG | + | chr1.1:76394142-76394161 | MS.gene96697:CDS | 65.0% |
| TCGCTCCGGGACACGGGACT+TGG | + | chr1.1:76394039-76394058 | MS.gene96697:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 76393748 | 76394455 | 76393748 | ID=MS.gene96697 |
| chr1.1 | mRNA | 76393748 | 76394455 | 76393748 | ID=MS.gene96697.t1;Parent=MS.gene96697 |
| chr1.1 | exon | 76393748 | 76394455 | 76393748 | ID=MS.gene96697.t1.exon1;Parent=MS.gene96697.t1 |
| chr1.1 | CDS | 76393748 | 76394455 | 76393748 | ID=cds.MS.gene96697.t1;Parent=MS.gene96697.t1 |
| Gene Sequence |
| Protein sequence |
