AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene062939

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062939.t1 MTR_4g072210 97.959 245 5 0 1 245 29 273 0.0 500
MS.gene062939.t1 MTR_5g021980 70.886 237 67 1 4 238 36 272 5.52e-127 361
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene062939.t1 AT5G45600 70.492 244 67 1 1 244 29 267 2.00e-136 384
MS.gene062939.t1 AT5G45600 70.492 244 66 1 1 244 29 266 3.30e-136 384
MS.gene062939.t1 AT2G18000 58.750 240 97 1 5 242 24 263 6.80e-105 305
MS.gene062939.t1 AT2G18000 60.920 174 66 1 5 176 24 197 1.00e-78 236

Find 54 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCTGCTTCTGAGAAATTC+AGG 0.264995 4.2:-35672027 None:intergenic
TATACGGGAACGTAGCATTT+TGG 0.267574 4.2:+35670778 MS.gene062939:CDS
CAAGGTATCAATCACATAAA+TGG 0.269072 4.2:+35670939 MS.gene062939:intron
TTCAGGAACCATTGGCTTAA+GGG 0.287646 4.2:-35672010 None:intergenic
ATTAGTGTTCCAATAGTATA+CGG 0.306773 4.2:+35670762 MS.gene062939:CDS
ATTCAGGAACCATTGGCTTA+AGG 0.353599 4.2:-35672011 None:intergenic
GCTACGTTCCCGTATACTAT+TGG 0.356357 4.2:-35670771 None:intergenic
AAGGAACTGCCTTCATCATC+TGG 0.371847 4.2:-35671705 None:intergenic
GCTGGCAGCAGCTCGTCAGC+AGG 0.387535 4.2:+35672060 MS.gene062939:CDS
AGTCTTGGCATGTTCACAAC+TGG 0.396171 4.2:-35671819 None:intergenic
AACAGGCTTCTTTGTGGAAA+AGG 0.415370 4.2:-35671724 None:intergenic
CACAACAACAGGCTTCTTTG+TGG 0.416649 4.2:-35671730 None:intergenic
CTGAGAAATTCAGGAACCAT+TGG 0.419880 4.2:-35672018 None:intergenic
TCATAAAATTCCACAACAAC+AGG 0.422701 4.2:-35671741 None:intergenic
GGAGGCAAGGTAAGCCCAGC+AGG 0.425533 4.2:-35671843 None:intergenic
CACAAAGAAGCCTGTTGTTG+TGG 0.444280 4.2:+35671731 MS.gene062939:CDS
TGGAGCATCCAACGAGGACC+TGG 0.447185 4.2:+35670976 MS.gene062939:CDS
TAATTATATATCTATTGGTC+AGG 0.454108 4.2:-35674288 None:intergenic
TGAGTTGTCAGAGGCAGGAT+GGG 0.486427 4.2:+35671078 MS.gene062939:CDS
TACCTTCTTATCATTGTCAT+CGG 0.494938 4.2:-35669910 None:intergenic
CGTTTACTAGCATCTTCGAT+TGG 0.501880 4.2:-35671962 None:intergenic
GTAAGCCCAGCAGGTAGTCT+TGG 0.502144 4.2:-35671834 None:intergenic
CAATCGAAGATGCTAGTAAA+CGG 0.502979 4.2:+35671963 MS.gene062939:CDS
TTGAGTTGTCAGAGGCAGGA+TGG 0.507623 4.2:+35671077 MS.gene062939:CDS
AGCAGATGAACTCTTGCAGC+TGG 0.510733 4.2:+35672042 MS.gene062939:CDS
TTACTCTTTGTGTAGAACTC+AGG 0.519723 4.2:+35670717 MS.gene062939:intron
ATCCGATGACAATGATAAGA+AGG 0.520538 4.2:+35669908 MS.gene062939:CDS
CCTGCCTCTGACAACTCAAA+AGG 0.523948 4.2:-35671073 None:intergenic
CAATAATCCCACAAGGATTG+TGG 0.525358 4.2:+35671042 MS.gene062939:CDS
GGAGCATCCAACGAGGACCT+GGG 0.547877 4.2:+35670977 MS.gene062939:CDS
GAGTTGTCAGAGGCAGGATG+GGG 0.549359 4.2:+35671079 MS.gene062939:CDS
GTCTTGGCATGTTCACAACT+GGG 0.560043 4.2:-35671818 None:intergenic
AAATGGACTATATATGTTCG+TGG 0.565195 4.2:+35670956 MS.gene062939:CDS
GGTGCATCCACAATCCTTGT+GGG 0.565259 4.2:-35671049 None:intergenic
AAGATAATCCCTTAAGCCAA+TGG 0.568475 4.2:+35672002 MS.gene062939:CDS
CAAAGTAACATACTAGGAGG+AGG 0.586112 4.2:-35671861 None:intergenic
GAGCATCCAACGAGGACCTG+GGG 0.590570 4.2:+35670978 MS.gene062939:CDS
AACATGCCAAGACTACCTGC+TGG 0.595578 4.2:+35671828 MS.gene062939:CDS
TTAGTGTTCCAATAGTATAC+GGG 0.596441 4.2:+35670763 MS.gene062939:CDS
TAACATACTAGGAGGAGGCA+AGG 0.606191 4.2:-35671856 None:intergenic
AGGTGCATCCACAATCCTTG+TGG 0.606632 4.2:-35671050 None:intergenic
CAAACGACTCACAAGTTCAC+AGG 0.610052 4.2:-35671154 None:intergenic
TATACAGATATCATCAGTTG+AGG 0.617329 4.2:+35671676 MS.gene062939:intron
GCCTCTGACAACTCAAAAGG+AGG 0.617900 4.2:-35671070 None:intergenic
CGACAGATAAGTTTGATCGA+TGG 0.622925 4.2:+35674240 MS.gene062939:CDS
ACATGCCAAGACTACCTGCT+GGG 0.629730 4.2:+35671829 MS.gene062939:CDS
AGGTTGTATCCAGATGATGA+AGG 0.632066 4.2:+35671696 MS.gene062939:CDS
CTAGTTTCAATAATCCCACA+AGG 0.653695 4.2:+35671035 MS.gene062939:CDS
GATGCTAGTAAACGGAGGAG+AGG 0.657501 4.2:+35671971 MS.gene062939:CDS
ACTGTGGAAATAAAGTGTGA+TGG 0.663057 4.2:-35671116 None:intergenic
AGCATCCAACGAGGACCTGG+GGG 0.700369 4.2:+35670979 MS.gene062939:CDS
TCGAAGATGCTAGTAAACGG+AGG 0.723176 4.2:+35671966 MS.gene062939:CDS
TGTTCGTGGAGCATCCAACG+AGG 0.768144 4.2:+35670970 MS.gene062939:CDS
GCTTGTCGCAAACATCACTG+TGG 0.771336 4.2:-35671132 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAGTAAAAGATAAAATAA+AGG - chr4.2:35670333-35670352 None:intergenic 10.0%
!! TTATCATTATTAATAAAATG+CGG - chr4.2:35671444-35671463 None:intergenic 10.0%
!!! ATTAAAAAGTTAGTTTAATT+AGG + chr4.2:35672985-35673004 MS.gene062939:intron 10.0%
!!! TTAAAAAGTTAGTTTAATTA+GGG + chr4.2:35672986-35673005 MS.gene062939:intron 10.0%
!!! TTTTTCTTAAATAAGATTAT+TGG + chr4.2:35672498-35672517 MS.gene062939:intron 10.0%
!! AATAAATGTATTGCTTAAAT+GGG + chr4.2:35670866-35670885 MS.gene062939:intron 15.0%
!! AATATGCTTAAATAAATTTC+TGG + chr4.2:35672744-35672763 MS.gene062939:intron 15.0%
!! ATCTATTCTAATTAATACAA+AGG - chr4.2:35672469-35672488 None:intergenic 15.0%
!! CAACTTAATAATAAAATACA+AGG - chr4.2:35673727-35673746 None:intergenic 15.0%
!! TAATAAATGTATTGCTTAAA+TGG + chr4.2:35670865-35670884 MS.gene062939:intron 15.0%
!! TTAATTAAAACAACAATTGA+AGG - chr4.2:35670844-35670863 None:intergenic 15.0%
!!! ATATTTTGAATAAAACCATT+TGG - chr4.2:35672563-35672582 None:intergenic 15.0%
!!! CTATTTTATCTTAGTTATTT+AGG + chr4.2:35672839-35672858 MS.gene062939:intron 15.0%
!! AAAGAAAATATTTAGGAAAG+AGG - chr4.2:35673050-35673069 None:intergenic 20.0%
!! AAAGCTTCAAATTTGAATAA+AGG - chr4.2:35670024-35670043 None:intergenic 20.0%
!! AAATTGATGAAAGATAATAG+AGG - chr4.2:35673344-35673363 None:intergenic 20.0%
!! AACAATGTTTATGTTATACA+AGG + chr4.2:35670921-35670940 MS.gene062939:intron 20.0%
!! AACATATAATCAAGAATCTT+CGG + chr4.2:35672343-35672362 MS.gene062939:intron 20.0%
!! AAGTTAATTCTATCATCAAA+AGG + chr4.2:35673827-35673846 MS.gene062939:intron 20.0%
!! AATTTGCACTGTATATTTAA+TGG + chr4.2:35673020-35673039 MS.gene062939:intron 20.0%
!! AGTTAATTCTATCATCAAAA+GGG + chr4.2:35673828-35673847 MS.gene062939:intron 20.0%
!! ATTATCTTTCATCAATTTAC+AGG + chr4.2:35673346-35673365 MS.gene062939:intron 20.0%
!! CAGAAATAAAAAGTAAATCA+TGG - chr4.2:35673620-35673639 None:intergenic 20.0%
!! GAAGAACAAAGAAAATATTT+AGG - chr4.2:35673057-35673076 None:intergenic 20.0%
!! TAAATCATTTAGTTACAAAC+AGG - chr4.2:35673267-35673286 None:intergenic 20.0%
!! TGTTCTAATAATATCTATGT+GGG + chr4.2:35672675-35672694 MS.gene062939:intron 20.0%
!! TTCAAATAAATGTATCATCA+TGG - chr4.2:35672151-35672170 None:intergenic 20.0%
!! TTGTTCTAATAATATCTATG+TGG + chr4.2:35672674-35672693 MS.gene062939:intron 20.0%
!!! ATTTTGACATTCACAAAATA+TGG - chr4.2:35671619-35671638 None:intergenic 20.0%
!!! TTTTACTCAAATAAGATTGT+AGG + chr4.2:35673213-35673232 MS.gene062939:intron 20.0%
! AAATAGTGTTTCATTAATCG+TGG + chr4.2:35673937-35673956 MS.gene062939:intron 25.0%
! AACAATTTACAGCAAGTATA+TGG + chr4.2:35672179-35672198 MS.gene062939:intron 25.0%
! AAGATGAAATAACTATCAGT+TGG - chr4.2:35674180-35674199 None:intergenic 25.0%
! AAGTACCATATGTAAAAGTA+AGG - chr4.2:35672910-35672929 None:intergenic 25.0%
! AATAAGCTAAAAACATCCAA+AGG - chr4.2:35670257-35670276 None:intergenic 25.0%
! AATGAATAACTTTCAAGCAT+TGG - chr4.2:35671537-35671556 None:intergenic 25.0%
! ACATCCATTATTATCAATTG+TGG + chr4.2:35673474-35673493 MS.gene062939:intron 25.0%
! ACGTTAATTAAACTCAAATG+TGG - chr4.2:35671920-35671939 None:intergenic 25.0%
! ATTAGTGTTCCAATAGTATA+CGG + chr4.2:35670762-35670781 MS.gene062939:CDS 25.0%
! CAATTCAATCAAGTCAATAA+GGG - chr4.2:35670479-35670498 None:intergenic 25.0%
! CATCCATTATTATCAATTGT+GGG + chr4.2:35673475-35673494 MS.gene062939:intron 25.0%
! CCATTATCATCAATATATTG+TGG + chr4.2:35672645-35672664 MS.gene062939:intron 25.0%
! GTTAATGATATGTAACAAAC+AGG - chr4.2:35671327-35671346 None:intergenic 25.0%
! GTTCTAATAATATCTATGTG+GGG + chr4.2:35672676-35672695 MS.gene062939:intron 25.0%
! TCAACGAATAATACAAAAGT+GGG - chr4.2:35672431-35672450 None:intergenic 25.0%
! TCAATTCAATCAAGTCAATA+AGG - chr4.2:35670480-35670499 None:intergenic 25.0%
! TCATCAATATATTGTGGATT+AGG + chr4.2:35672651-35672670 MS.gene062939:intron 25.0%
! TGATTATATGTTTAGAAGTG+TGG - chr4.2:35672335-35672354 None:intergenic 25.0%
! TGTTCTAATAATTTCTATGC+CGG + chr4.2:35673505-35673524 MS.gene062939:intron 25.0%
! TTATTTGAGCTTATCTATTG+AGG + chr4.2:35670506-35670525 MS.gene062939:intron 25.0%
! TTGTGATTGTTCATCATTTA+AGG + chr4.2:35672607-35672626 MS.gene062939:intron 25.0%
!! ATCATTTAAGGATTTGTTTC+TGG + chr4.2:35672619-35672638 MS.gene062939:intron 25.0%
!! CCACAATATATTGATGATAA+TGG - chr4.2:35672648-35672667 None:intergenic 25.0%
!!! CTTAAATAAATTTCTGGTCT+TGG + chr4.2:35672750-35672769 MS.gene062939:intron 25.0%
!!! GAAGTACACAATTTTTTGTA+AGG - chr4.2:35672800-35672819 None:intergenic 25.0%
!!! GTTGTAAAAAGCGTTATTTT+GGG - chr4.2:35669967-35669986 None:intergenic 25.0%
!!! GTTGTTTTCATAAACTATTC+AGG - chr4.2:35670292-35670311 None:intergenic 25.0%
!!! GTTGTTTTTGATTTAGTCTT+TGG + chr4.2:35671179-35671198 MS.gene062939:intron 25.0%
!!! TAGTTATTTCATCTTTTCCA+TGG + chr4.2:35674185-35674204 MS.gene062939:intron 25.0%
AACAGTGAATAATAGACCTA+TGG - chr4.2:35671575-35671594 None:intergenic 30.0%
AACTCTACTAATCCCAAAAT+TGG - chr4.2:35673162-35673181 None:intergenic 30.0%
AAGCATTTGTGATACTCTTT+GGG + chr4.2:35670224-35670243 MS.gene062939:intron 30.0%
ATGTACAATCGACATTTCTA+GGG - chr4.2:35672266-35672285 None:intergenic 30.0%
CAATTGCAAAGTAACATACT+AGG - chr4.2:35671870-35671889 None:intergenic 30.0%
CCATGATAGCTTAATGAAAA+TGG + chr4.2:35670613-35670632 MS.gene062939:intron 30.0%
GGTTAAACTACTTAGATACA+AGG + chr4.2:35673857-35673876 MS.gene062939:intron 30.0%
GTCAACGAATAATACAAAAG+TGG - chr4.2:35672432-35672451 None:intergenic 30.0%
GTTAGATTGAGTTAGTAGTT+TGG + chr4.2:35671406-35671425 MS.gene062939:intron 30.0%
GTTCTAATAATTTCTATGCC+GGG + chr4.2:35673506-35673525 MS.gene062939:intron 30.0%
TAAGCATTTGTGATACTCTT+TGG + chr4.2:35670223-35670242 MS.gene062939:intron 30.0%
TACAGACTTCATTATGAGTA+AGG - chr4.2:35672211-35672230 None:intergenic 30.0%
TACCATATGTAAAAGTAAGG+TGG - chr4.2:35672907-35672926 None:intergenic 30.0%
TACCTTCTTATCATTGTCAT+CGG - chr4.2:35669913-35669932 None:intergenic 30.0%
TATACAGATATCATCAGTTG+AGG + chr4.2:35671676-35671695 MS.gene062939:intron 30.0%
TCAAAGTATGCTCAAAAGTA+GGG + chr4.2:35671277-35671296 MS.gene062939:intron 30.0%
TCATAAAATTCCACAACAAC+AGG - chr4.2:35671744-35671763 None:intergenic 30.0%
TCATGGAAACAGTATATCTT+TGG - chr4.2:35673603-35673622 None:intergenic 30.0%
TGATATTACTACATGACCAT+AGG + chr4.2:35671556-35671575 MS.gene062939:intron 30.0%
TTAGTGTTCCAATAGTATAC+GGG + chr4.2:35670763-35670782 MS.gene062939:CDS 30.0%
TTCAAAGTATGCTCAAAAGT+AGG + chr4.2:35671276-35671295 MS.gene062939:intron 30.0%
TTCACACAAAAAATGTGCTA+AGG + chr4.2:35670897-35670916 MS.gene062939:intron 30.0%
TTTATGCACTCCGAAAAAAA+GGG - chr4.2:35669994-35670013 None:intergenic 30.0%
! AACTACTTTGTTTACCCAAA+TGG - chr4.2:35673778-35673797 None:intergenic 30.0%
! CAAGGTATCAATCACATAAA+TGG + chr4.2:35670939-35670958 MS.gene062939:intron 30.0%
! CCATTTTCATTAAGCTATCA+TGG - chr4.2:35670616-35670635 None:intergenic 30.0%
! CTATTGAGGTAAACACTTTT+AGG + chr4.2:35670520-35670539 MS.gene062939:intron 30.0%
! TGACCCACAATTGATAATAA+TGG - chr4.2:35673481-35673500 None:intergenic 30.0%
! TGTTGTGGAATTTTATGATG+AGG + chr4.2:35671746-35671765 MS.gene062939:CDS 30.0%
!! AAATGGACTATATATGTTCG+TGG + chr4.2:35670956-35670975 MS.gene062939:CDS 30.0%
!! TTCCACCTTACTTTTACATA+TGG + chr4.2:35672902-35672921 MS.gene062939:intron 30.0%
!!! ACTTTATTTTCCCCATAAAC+AGG - chr4.2:35671208-35671227 None:intergenic 30.0%
!!! AGGTAAACACTTTTAGGTTT+GGG + chr4.2:35670526-35670545 MS.gene062939:intron 30.0%
!!! CCTTTGTTTTCATTCAGATT+CGG + chr4.2:35669934-35669953 MS.gene062939:intron 30.0%
!!! GCTTCAACTCTATTTTTGTT+TGG + chr4.2:35673123-35673142 MS.gene062939:intron 30.0%
!!! GGTTGTAAAAAGCGTTATTT+TGG - chr4.2:35669968-35669987 None:intergenic 30.0%
!!! TACAACCATACCCTTTTTTT+CGG + chr4.2:35669981-35670000 MS.gene062939:intron 30.0%
!!! TTAGCACATTTTTTGTGTGA+AGG - chr4.2:35670898-35670917 None:intergenic 30.0%
AAGATAATCCCTTAAGCCAA+TGG + chr4.2:35672002-35672021 MS.gene062939:CDS 35.0%
ACTGAACATGGTCAATGATT+TGG - chr4.2:35673676-35673695 None:intergenic 35.0%
ACTGTGGAAATAAAGTGTGA+TGG - chr4.2:35671119-35671138 None:intergenic 35.0%
AGAGTTTAGCATACTGAACA+TGG - chr4.2:35673688-35673707 None:intergenic 35.0%
AGATCACAGTATCACACAAA+AGG - chr4.2:35671238-35671257 None:intergenic 35.0%
ATCATACATGTACACGTGTT+TGG + chr4.2:35672230-35672249 MS.gene062939:intron 35.0%
ATCCGATGACAATGATAAGA+AGG + chr4.2:35669908-35669927 MS.gene062939:CDS 35.0%
ATCCTTTAATGAACAAGCAC+AGG - chr4.2:35673445-35673464 None:intergenic 35.0%
CAATCGAAGATGCTAGTAAA+CGG + chr4.2:35671963-35671982 MS.gene062939:CDS 35.0%
CAATCTAACTGAACTTAGCA+GGG - chr4.2:35671395-35671414 None:intergenic 35.0%
CATGTACAATCGACATTTCT+AGG - chr4.2:35672267-35672286 None:intergenic 35.0%
CCGAATCTGAATGAAAACAA+AGG - chr4.2:35669937-35669956 None:intergenic 35.0%
CTAGTTTCAATAATCCCACA+AGG + chr4.2:35671035-35671054 MS.gene062939:CDS 35.0%
CTATCATCAAAAGGGAAATG+TGG + chr4.2:35673836-35673855 MS.gene062939:intron 35.0%
CTTTATGCACTCCGAAAAAA+AGG - chr4.2:35669995-35670014 None:intergenic 35.0%
GATCACAGTATCACACAAAA+GGG - chr4.2:35671237-35671256 None:intergenic 35.0%
TAAAAAAGCTTCAGAGGGAT+CGG - chr4.2:35671781-35671800 None:intergenic 35.0%
TCAATCTAACTGAACTTAGC+AGG - chr4.2:35671396-35671415 None:intergenic 35.0%
TGATGAACAATCACAAGAGA+TGG - chr4.2:35672603-35672622 None:intergenic 35.0%
TGTATCAACTTCCACAAATG+AGG - chr4.2:35674058-35674077 None:intergenic 35.0%
TTACTCTTTCTTGACGACTT+TGG + chr4.2:35674115-35674134 MS.gene062939:intron 35.0%
TTACTCTTTGTGTAGAACTC+AGG + chr4.2:35670717-35670736 MS.gene062939:intron 35.0%
TTGCAAAGTAACATACTAGG+AGG - chr4.2:35671867-35671886 None:intergenic 35.0%
! AGCATTTTGAATATTCAGCC+CGG - chr4.2:35673527-35673546 None:intergenic 35.0%
! ATGATGCTTACGTCCAATTT+TGG + chr4.2:35673146-35673165 MS.gene062939:intron 35.0%
! GATTCTTGTTTGTGACCAAA+TGG + chr4.2:35672545-35672564 MS.gene062939:intron 35.0%
! TGATGCTTACGTCCAATTTT+GGG + chr4.2:35673147-35673166 MS.gene062939:intron 35.0%
!!! GAGGTAAACACTTTTAGGTT+TGG + chr4.2:35670525-35670544 MS.gene062939:intron 35.0%
AAAAAAGCTTCAGAGGGATC+GGG - chr4.2:35671780-35671799 None:intergenic 40.0%
AACAGGCTTCTTTGTGGAAA+AGG - chr4.2:35671727-35671746 None:intergenic 40.0%
AATCTTCTCCTGACCAATAG+AGG + chr4.2:35674280-35674299 MS.gene062939:CDS 40.0%
ACGAGCTAAAAAAGCTTCAG+AGG - chr4.2:35671787-35671806 None:intergenic 40.0%
AGGTTGTATCCAGATGATGA+AGG + chr4.2:35671696-35671715 MS.gene062939:CDS 40.0%
AGTCTTTGGTTGCCTGTTTA+TGG + chr4.2:35671193-35671212 MS.gene062939:intron 40.0%
CAAAGTAACATACTAGGAGG+AGG - chr4.2:35671864-35671883 None:intergenic 40.0%
CAAGAATCCTTTACATGCAC+AGG - chr4.2:35672533-35672552 None:intergenic 40.0%
CAATAATCCCACAAGGATTG+TGG + chr4.2:35671042-35671061 MS.gene062939:CDS 40.0%
CACCTGTGCTTGTTCATTAA+AGG + chr4.2:35673440-35673459 MS.gene062939:intron 40.0%
CGACAGATAAGTTTGATCGA+TGG + chr4.2:35674240-35674259 MS.gene062939:CDS 40.0%
CGAGCTAAAAAAGCTTCAGA+GGG - chr4.2:35671786-35671805 None:intergenic 40.0%
CGTTTACTAGCATCTTCGAT+TGG - chr4.2:35671965-35671984 None:intergenic 40.0%
CTGAGAAATTCAGGAACCAT+TGG - chr4.2:35672021-35672040 None:intergenic 40.0%
GTAGTTACCTGTGCATGTAA+AGG + chr4.2:35672523-35672542 MS.gene062939:intron 40.0%
GTATGCTAAACTCTGTGTGA+TGG + chr4.2:35673694-35673713 MS.gene062939:intron 40.0%
GTCTTTGGTTGCCTGTTTAT+GGG + chr4.2:35671194-35671213 MS.gene062939:intron 40.0%
TCTGTGATCATGCTCTATAG+AGG + chr4.2:35673636-35673655 MS.gene062939:intron 40.0%
TCTTTGGTTGCCTGTTTATG+GGG + chr4.2:35671195-35671214 MS.gene062939:intron 40.0%
TGCTTATGTGTCCTCATTTG+TGG + chr4.2:35674044-35674063 MS.gene062939:intron 40.0%
TGTAAAGTCTTGCAGTACTG+TGG + chr4.2:35671362-35671381 MS.gene062939:intron 40.0%
! CATCTGCTTCTGAGAAATTC+AGG - chr4.2:35672030-35672049 None:intergenic 40.0%
! CTCTTTGGGAAGATTTCCTT+TGG + chr4.2:35670238-35670257 MS.gene062939:intron 40.0%
! TATACGGGAACGTAGCATTT+TGG + chr4.2:35670778-35670797 MS.gene062939:CDS 40.0%
! TTTAGGATCCCTCCATTACA+TGG + chr4.2:35672856-35672875 MS.gene062939:intron 40.0%
! TTTAGGTTTGGGAGAACTGA+TGG + chr4.2:35670537-35670556 MS.gene062939:intron 40.0%
! TTTCATCTTTTCCATGGCGT+AGG + chr4.2:35674191-35674210 MS.gene062939:intron 40.0%
!! ATTCAGGAACCATTGGCTTA+AGG - chr4.2:35672014-35672033 None:intergenic 40.0%
!! TTCAGGAACCATTGGCTTAA+GGG - chr4.2:35672013-35672032 None:intergenic 40.0%
AAAAAGCTTCAGAGGGATCG+GGG - chr4.2:35671779-35671798 None:intergenic 45.0%
AAGGAACTGCCTTCATCATC+TGG - chr4.2:35671708-35671727 None:intergenic 45.0%
AGTCTTGGCATGTTCACAAC+TGG - chr4.2:35671822-35671841 None:intergenic 45.0%
CAAACGACTCACAAGTTCAC+AGG - chr4.2:35671157-35671176 None:intergenic 45.0%
CACAAAGAAGCCTGTTGTTG+TGG + chr4.2:35671731-35671750 MS.gene062939:CDS 45.0%
CACAACAACAGGCTTCTTTG+TGG - chr4.2:35671733-35671752 None:intergenic 45.0%
GATCTCTTGCTTCAGTGTAG+AGG + chr4.2:35671253-35671272 MS.gene062939:intron 45.0%
GCACTCCGAAAAAAAGGGTA+TGG - chr4.2:35669989-35670008 None:intergenic 45.0%
GCTACGTTCCCGTATACTAT+TGG - chr4.2:35670774-35670793 None:intergenic 45.0%
GTCTTGGCATGTTCACAACT+GGG - chr4.2:35671821-35671840 None:intergenic 45.0%
TAACATACTAGGAGGAGGCA+AGG - chr4.2:35671859-35671878 None:intergenic 45.0%
TCGAAGATGCTAGTAAACGG+AGG + chr4.2:35671966-35671985 MS.gene062939:CDS 45.0%
TGTGGAAAAATGAAAGCCGC+AGG - chr4.2:35671902-35671921 None:intergenic 45.0%
! GGCAACACAGTTTTCTCACA+AGG - chr4.2:35674159-35674178 None:intergenic 45.0%
!! CAAGTTGATTGCTCGAGTTG+TGG + chr4.2:35673751-35673770 MS.gene062939:intron 45.0%
!!! ACCTCCTTTTGAGTTGTCAG+AGG + chr4.2:35671069-35671088 MS.gene062939:CDS 45.0%
!!! TTTAAAATATATAATATTTG+TGG + chr4.2:35672104-35672123 MS.gene062939:intron 5.0%
AACATGCCAAGACTACCTGC+TGG + chr4.2:35671828-35671847 MS.gene062939:CDS 50.0%
ACATGCCAAGACTACCTGCT+GGG + chr4.2:35671829-35671848 MS.gene062939:CDS 50.0%
AGCAGATGAACTCTTGCAGC+TGG + chr4.2:35672042-35672061 MS.gene062939:CDS 50.0%
AGGTGCATCCACAATCCTTG+TGG - chr4.2:35671053-35671072 None:intergenic 50.0%
ATGAAAGCCGCAGGTGACTA+TGG - chr4.2:35671893-35671912 None:intergenic 50.0%
ATGAGCTTGAACCTACGCCA+TGG - chr4.2:35674205-35674224 None:intergenic 50.0%
CACGCTTTACAATAACCCCC+AGG - chr4.2:35670997-35671016 None:intergenic 50.0%
CAGGAATTCCTCTCATGCAC+AGG - chr4.2:35673248-35673267 None:intergenic 50.0%
CCTGCCTCTGACAACTCAAA+AGG - chr4.2:35671076-35671095 None:intergenic 50.0%
GATCCCTCCATTACATGGTG+AGG + chr4.2:35672861-35672880 MS.gene062939:intron 50.0%
GATGCTAGTAAACGGAGGAG+AGG + chr4.2:35671971-35671990 MS.gene062939:CDS 50.0%
GCAATTGCCATAGTCACCTG+CGG + chr4.2:35671883-35671902 MS.gene062939:intron 50.0%
GCCTCTGACAACTCAAAAGG+AGG - chr4.2:35671073-35671092 None:intergenic 50.0%
GCTTGTCGCAAACATCACTG+TGG - chr4.2:35671135-35671154 None:intergenic 50.0%
GGTGCATCCACAATCCTTGT+GGG - chr4.2:35671052-35671071 None:intergenic 50.0%
TCAGCCACCTCACCATGTAA+TGG - chr4.2:35672871-35672890 None:intergenic 50.0%
TGAGTTGTCAGAGGCAGGAT+GGG + chr4.2:35671078-35671097 MS.gene062939:CDS 50.0%
! CCACCTCACCATGTAATGGA+GGG - chr4.2:35672867-35672886 None:intergenic 50.0%
! GCTCGAGTTGTGGTCCATTT+GGG + chr4.2:35673761-35673780 MS.gene062939:intron 50.0%
! TGCTCGAGTTGTGGTCCATT+TGG + chr4.2:35673760-35673779 MS.gene062939:intron 50.0%
! TGTACTGACCTGTGCATGAG+AGG + chr4.2:35673237-35673256 MS.gene062939:intron 50.0%
! TTGAGTTGTCAGAGGCAGGA+TGG + chr4.2:35671077-35671096 MS.gene062939:CDS 50.0%
!!! CCTTTTGAGTTGTCAGAGGC+AGG + chr4.2:35671073-35671092 MS.gene062939:CDS 50.0%
!!! GGGAACGTAGCATTTTGGCT+TGG + chr4.2:35670783-35670802 MS.gene062939:CDS 50.0%
AATAACCCCCAGGTCCTCGT+TGG - chr4.2:35670987-35671006 None:intergenic 55.0%
CAGCTCGTCAGCAGGTATGA+TGG + chr4.2:35672068-35672087 MS.gene062939:intron 55.0%
CCCTCCATTACATGGTGAGG+TGG + chr4.2:35672864-35672883 MS.gene062939:intron 55.0%
GAGTTGTCAGAGGCAGGATG+GGG + chr4.2:35671079-35671098 MS.gene062939:CDS 55.0%
GCCACCTCACCATGTAATGG+AGG - chr4.2:35672868-35672887 None:intergenic 55.0%
GTAAGCCCAGCAGGTAGTCT+TGG - chr4.2:35671837-35671856 None:intergenic 55.0%
! TGTTCGTGGAGCATCCAACG+AGG + chr4.2:35670970-35670989 MS.gene062939:CDS 55.0%
AGCATCCAACGAGGACCTGG+GGG + chr4.2:35670979-35670998 MS.gene062939:CDS 60.0%
GAGCATCCAACGAGGACCTG+GGG + chr4.2:35670978-35670997 MS.gene062939:CDS 60.0%
! GGAGCATCCAACGAGGACCT+GGG + chr4.2:35670977-35670996 MS.gene062939:CDS 60.0%
! TGGAGCATCCAACGAGGACC+TGG + chr4.2:35670976-35670995 MS.gene062939:CDS 60.0%
GGAGGCAAGGTAAGCCCAGC+AGG - chr4.2:35671846-35671865 None:intergenic 65.0%
GCTGGCAGCAGCTCGTCAGC+AGG + chr4.2:35672060-35672079 MS.gene062939:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 35669900 35674299 35669900 ID=MS.gene062939
chr4.2 mRNA 35669900 35674299 35669900 ID=MS.gene062939.t1;Parent=MS.gene062939
chr4.2 exon 35669900 35669929 35669900 ID=MS.gene062939.t1.exon1;Parent=MS.gene062939.t1
chr4.2 CDS 35669900 35669929 35669900 ID=cds.MS.gene062939.t1;Parent=MS.gene062939.t1
chr4.2 exon 35670732 35670820 35670732 ID=MS.gene062939.t1.exon2;Parent=MS.gene062939.t1
chr4.2 CDS 35670732 35670820 35670732 ID=cds.MS.gene062939.t1;Parent=MS.gene062939.t1
chr4.2 exon 35670943 35671164 35670943 ID=MS.gene062939.t1.exon3;Parent=MS.gene062939.t1
chr4.2 CDS 35670943 35671164 35670943 ID=cds.MS.gene062939.t1;Parent=MS.gene062939.t1
chr4.2 exon 35671683 35671870 35671683 ID=MS.gene062939.t1.exon4;Parent=MS.gene062939.t1
chr4.2 CDS 35671683 35671870 35671683 ID=cds.MS.gene062939.t1;Parent=MS.gene062939.t1
chr4.2 exon 35671960 35672081 35671960 ID=MS.gene062939.t1.exon5;Parent=MS.gene062939.t1
chr4.2 CDS 35671960 35672081 35671960 ID=cds.MS.gene062939.t1;Parent=MS.gene062939.t1
chr4.2 exon 35674213 35674299 35674213 ID=MS.gene062939.t1.exon6;Parent=MS.gene062939.t1
chr4.2 CDS 35674213 35674299 35674213 ID=cds.MS.gene062939.t1;Parent=MS.gene062939.t1
Gene Sequence

>MS.gene062939

ATGGGCAAATCCGATGACAATGATAAGAAGGTAACCTTTGTTTTCATTCAGATTCGGTTAAAATCCCAAAATAACGCTTTTTACAACCATACCCTTTTTTTCGGAGTGCATAAAGTTTGCACCTTTATTCAAATTTGAAGCTTTGATTTGATACAAACTTATCACACTGCAATAACTTGAAATCAATCTGTAGAATGTTAAATTTGAAAATGATTTTTGAATGTGAATGCAATGTGAAGAGAAATTTTTCTATTTACTGAAGTAGAATCTCTTATTGATAATGAGCTTGATTGAATTGAATCAGTTAAGCTTATTTACTGATATAAGCATTTGTGATACTCTTTGGGAAGATTTCCTTTGGATGTTTTTAGCTTATTTCAATAAGCTTTCCTGAATAGTTTATGAAAACAACATATATGCAAATAGTTTGCCTTTATTTTATCTTTTACTATAGAAATAGTTTAAGAATAAGCACTTACATTACTATAAGCGTTAAATTAATTTGTTTATTCGAACATGTCGTATATTATCTGAGCTTGATTTAATTGTTCAAATGAGTTTTTGATCTTGTGTCGACCCTTATTGACTTGATTGAATTGAATTGAATTATTTGAGCTTATCTATTGAGGTAAACACTTTTAGGTTTGGGAGAACTGATGGAAACAACTTATAACATTTTCTTAAGCTATTTTCAGCTTATTTATATAAGTTTTCCATGATAGCTTAATGAAAATGGCTTATAGTTTGTAGCTTGTATGAAAACAATTTGTCTTTATTTTATCTTTTGTTACATAAAAAGCTTAATTAAGTGCTTTTATTACTCTTTGTGTAGAACTCAGGTAAGAAACTCAAAGATGTAGAAATTAGTGTTCCAATAGTATACGGGAACGTAGCATTTTGGCTTGGCAAAAAAGCTAGTGAGTAAGTAACTTACATTGCATCCTTCAATTGTTGTTTTAATTAATTAATAAATGTATTGCTTAAATGGGTTTTTTCCTTCACACAAAAAATGTGCTAAGGTAACAATGTTTATGTTATACAAGGTATCAATCACATAAATGGACTATATATGTTCGTGGAGCATCCAACGAGGACCTGGGGGTTATTGTAAAGCGTGTTGTTTTTCAGTTGCATACTAGTTTCAATAATCCCACAAGGATTGTGGATGCACCTCCTTTTGAGTTGTCAGAGGCAGGATGGGGAGAATTTGAAATTGCCATCACACTTTATTTCCACAGTGATGTTTGCGACAAGCCTGTGAACTTGTGAGTCGTTTGATGTTGTTTTTGATTTAGTCTTTGGTTGCCTGTTTATGGGGAAAATAAAGTTTCTTACCCTTTTGTGTGATACTGTGATCTCTTGCTTCAGTGTAGAGGTTCAAAGTATGCTCAAAAGTAGGGAAAATCTCAAATAGTGTTTTAAGTCCTGTTTGTTACATATCATTAACTATGTGCTTTTGTTTTGTAAAGTCTTGCAGTACTGTGGCTCTATGCCCTGCTAAGTTCAGTTAGATTGAGTTAGTAGTTTGGTTTGCTTTTGTGCCGCATTTTATTAATAATGATAAGCTTAAATGATTATAAAACAACTAATATCTATCGTGTTATGTTTTTATTAGAGCATGCAGCTATACGATTCCAATGCTTGAAAGTTATTCATTGATATTACTACATGACCATAGGTCTATTATTCACTGTTATAAATCATGAAGATTGCAAACCATATTTTGTGAATGTCAAAATAATTTCTGAATCTTGTTTTTTATTGAAGTGTAATGTATATACAGATATCATCAGTTGAGGTTGTATCCAGATGATGAAGGCAGTTCCTTTTCCACAAAGAAGCCTGTTGTTGTGGAATTTTATGATGAGGTAGTTTTCCCCGATCCCTCTGAAGCTTTTTTAGCTCGTGTGCAGAATCACCCAGTTGTGAACATGCCAAGACTACCTGCTGGGCTTACCTTGCCTCCTCCTAGTATGTTACTTTGCAATTGCCATAGTCACCTGCGGCTTTCATTTTTCCACATTTGAGTTTAATTAACGTTACATTATCTATAAATGCAGTTCCAATCGAAGATGCTAGTAAACGGAGGAGAGGTGATACTAAAGATAATCCCTTAAGCCAATGGTTCCTGAATTTCTCAGAAGCAGATGAACTCTTGCAGCTGGCAGCAGCTCGTCAGCAGGTATGATGGACATAATATCATATTTAAAATATATAATATTTGTGGAAGTCGAAGTAGTTGTTGTTGCCATGATGATACATTTATTTGAATTTGAATCAACAATTTACAGCAAGTATATGGTTGCATCCTTACTCATAATGAAGTCTGTATCATACATGTACACGTGTTTGGCTGTTTATTACCCTAGAAATGTCGATTGTACATGAGTGTGATAGCATCAAGTTTTATGATTTTGTTCTAAGTATTCTCTCCACACTTCTAAACATATAATCAAGAATCTTCGGTTAAAGATTCAAAACTTCTGTTCTATACTTGAAGTTTATTTTTGTTTACGTGATGCTTATGACCCACTTTTGTATTATTCGTTGACTCAAAATGATTATGCCTTTGTATTAATTAGAATAGATTGCGTCTCATTTTTCTTAAATAAGATTATTGGTTGTAGTTACCTGTGCATGTAAAGGATTCTTGTTTGTGACCAAATGGTTTTATTCAAAATATGTCATAGTTATCTTGTACCATCTCTTGTGATTGTTCATCATTTAAGGATTTGTTTCTGGCATCCATTATCATCAATATATTGTGGATTAGGTTGTTCTAATAATATCTATGTGGGGCATGAATGTTCAGAGTGTATATCATAATACTATTTTATACTAATAAATATGCTTAAATAAATTTCTGGTCTTGGTAATAGAAGCTTTTCAGCTTTGTTCCTTACAAAAAATTGTGTACTTCTATATGAAGAAAGAAAAATCTATTTTATCTTAGTTATTTAGGATCCCTCCATTACATGGTGAGGTGGCTGACAGTGACATGATTCCACCTTACTTTTACATATGGTACTTATTTGTTCTTTATTTAAAATTTATATAATATTTGTATAAATAAACATAAATAGAAATTAAAAAGTTAGTTTAATTAGGGTTAAAAGTTAGAATTTGCACTGTATATTTAATGGAGAACCTCTTTCCTAAATATTTTCTTTGTTCTTCGTTCATAACATATAATGATGACTTATCAGTCAAAACTTTTTTCTATGCTTCAACTCTATTTTTGTTTGGATGATGCTTACGTCCAATTTTGGGATTAGTAGAGTTAAAATAATTGTCTTCGAATTATTAGTACAAATTTTACTCAAATAAGATTGTAGGTTGTACTGACCTGTGCATGAGAGGAATTCCTGTTTGTAACTAAATGATTTAATTCAACTTCTAAAGAAAGAGCGTGTTCTTCATTGAAGAGTTTGCTTCTGTCATCCTCTATTATCTTTCATCAATTTACAGGTCAGAGAGAAAAGACATTGTACATGCAAATTAACATTATTTCAATTTTATGTAATAGCTATCTCGTACTATCACCTGTGCTTGTTCATTAAAGGATTTGCTTCTGACATCCATTATTATCAATTGTGGGTCATGTGTGTTCTAATAATTTCTATGCCGGGCTGAATATTCAAAATGCTTATCATAATGCTCTAAATAATAAATACTAGTAAGTCATTCTATGTCATTAAGACCAAAGATATACTGTTTCCATGATTTACTTTTTATTTCTGTGATCATGCTCTATAGAGGAACTTATTTTTTCTCCAAATCATTGACCATGTTCAGTATGCTAAACTCTGTGTGATGGTTCTTTTCCTTGTATTTTATTATTAAGTTGTTGTCAAGTTGATTGCTCGAGTTGTGGTCCATTTGGGTAAACAAAGTAGTTAAGTGCTTATCATATAAGCGTTTATGAATAAGTTAATTCTATCATCAAAAGGGAAATGTGGTTAAACTACTTAGATACAAGGTGTATGTTTTCAAAAATCACAGGCTATTGTTTATAATTTACTGAAACAAGTCGACGAAAATAGTGTTTCATTAATCGTGGACTAGTCATAAACTGTTTCATGAGTTAACTCAAACTACACGAGTGCTTATGTTATAAGATAAGCTTAAATAAGCTAATTATTGATGCTTATGTGTCCTCATTTGTGGAAGTTGATACATGCTGAATAATACTTGTTGCACAAATTATAATATGCTTTACTCTTTCTTGACGACTTTGGATGACTCTATCATCTTAACCTTGTGAGAAAACTGTGTTGCCAACTGATAGTTATTTCATCTTTTCCATGGCGTAGGTTCAAGCTCATATTTCAAAACTCAAACGACAGATAAGTTTGATCGATGGACAGCATAAACAGCTTAAATCTTCTCCTGACCAATAG
Protein sequence

>MS.gene062939.t1

MGKSDDNDKKNSGKKLKDVEISVPIVYGNVAFWLGKKASEYQSHKWTIYVRGASNEDLGVIVKRVVFQLHTSFNNPTRIVDAPPFELSEAGWGEFEIAITLYFHSDVCDKPVNLYHQLRLYPDDEGSSFSTKKPVVVEFYDEVVFPDPSEAFLARVQNHPVVNMPRLPAGLTLPPPIPIEDASKRRRGDTKDNPLSQWFLNFSEADELLQLAAARQQVQAHISKLKRQISLIDGQHKQLKSSPDQ