AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23366

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23366.t1 MTR_4g072210 97.436 273 7 0 1 273 1 273 0.0 551
MS.gene23366.t1 MTR_5g021980 70.886 237 67 1 32 266 36 272 4.41e-127 362
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23366.t1 AT5G45600 68.015 272 82 1 1 272 1 267 6.97e-143 402
MS.gene23366.t1 AT5G45600 68.015 272 81 1 1 272 1 266 1.20e-142 401
MS.gene23366.t1 AT2G18000 58.750 240 97 1 33 270 24 263 9.86e-105 305
MS.gene23366.t1 AT2G18000 58.889 180 72 1 27 204 18 197 1.18e-78 237

Find 61 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCTGCTTCTGAGAAATTC+AGG 0.264995 4.4:-36916136 None:intergenic
TATTTCTCACCCAATCAAAA+TGG 0.267469 4.4:+36913993 MS.gene23366:CDS
TATACGGGAACGTAGCATTT+TGG 0.267574 4.4:+36914888 MS.gene23366:CDS
CAAGGTATCAATCACATAAA+TGG 0.269072 4.4:+36915049 MS.gene23366:intron
TTCAGGAACCATTGGCTTAA+GGG 0.287646 4.4:-36916119 None:intergenic
ATTAGTGTTCCAATAGTATA+CGG 0.306773 4.4:+36914872 MS.gene23366:CDS
ATTCAGGAACCATTGGCTTA+AGG 0.353599 4.4:-36916120 None:intergenic
GCTACGTTCCCGTATACTAT+TGG 0.356357 4.4:-36914881 None:intergenic
AAGGAACTGCCTTCATCATC+TGG 0.371847 4.4:-36915814 None:intergenic
GCTGGCAGCAGCTCGTCAGC+AGG 0.387535 4.4:+36916169 MS.gene23366:CDS
AGTCTTGGCATGTTCACAAC+TGG 0.396171 4.4:-36915928 None:intergenic
AACAGGCTTCTTTGTGGAAA+AGG 0.415370 4.4:-36915833 None:intergenic
CACAACAACAGGCTTCTTTG+TGG 0.416649 4.4:-36915839 None:intergenic
CTGAGAAATTCAGGAACCAT+TGG 0.419880 4.4:-36916127 None:intergenic
TCATAAAATTCCACAACAAC+AGG 0.422701 4.4:-36915850 None:intergenic
GGAGGCAAGGTAAGCCCAGC+AGG 0.425533 4.4:-36915952 None:intergenic
CACAAAGAAGCCTGTTGTTG+TGG 0.444280 4.4:+36915840 MS.gene23366:CDS
TGGAGCATCCAACGAGGACC+TGG 0.447185 4.4:+36915086 MS.gene23366:CDS
TAATTATATATCTATTGGTC+AGG 0.454108 4.4:-36918397 None:intergenic
TGAGTTGTCAGAGGCAGGAT+GGG 0.486427 4.4:+36915188 MS.gene23366:CDS
TACCTTCTTATCATTGTCAT+CGG 0.494938 4.4:-36914022 None:intergenic
CGTTTACTAGCATCTTCGAT+TGG 0.501880 4.4:-36916071 None:intergenic
GTAAGCCCAGCAGGTAGTCT+TGG 0.502144 4.4:-36915943 None:intergenic
CAATCGAAGATGCTAGTAAA+CGG 0.502979 4.4:+36916072 MS.gene23366:CDS
TTGAGTTGTCAGAGGCAGGA+TGG 0.507623 4.4:+36915187 MS.gene23366:CDS
ATTTCTCACCCAATCAAAAT+GGG 0.508818 4.4:+36913994 MS.gene23366:CDS
AGCAGATGAACTCTTGCAGC+TGG 0.510733 4.4:+36916151 MS.gene23366:CDS
TATTACTCTGTGTAGAACTC+AGG 0.519723 4.4:+36914827 MS.gene23366:intron
ATCCGATGACAATGATAAGA+AGG 0.520538 4.4:+36914020 MS.gene23366:CDS
CCTGCCTCTGACAACTCAAA+AGG 0.523948 4.4:-36915183 None:intergenic
CAATAATCCCACAAGGATTG+TGG 0.525358 4.4:+36915152 MS.gene23366:CDS
CAAGCTCAGTCTCAATTAAG+TGG 0.527609 4.4:+36913961 MS.gene23366:CDS
TTTGATTGGGTGAGAAATAG+GGG 0.531862 4.4:-36913989 None:intergenic
GGAGCATCCAACGAGGACCT+GGG 0.547877 4.4:+36915087 MS.gene23366:CDS
GAGTTGTCAGAGGCAGGATG+GGG 0.549359 4.4:+36915189 MS.gene23366:CDS
GTCTTGGCATGTTCACAACT+GGG 0.560043 4.4:-36915927 None:intergenic
AAATGGACTATATATGTTCG+TGG 0.565195 4.4:+36915066 MS.gene23366:CDS
GGTGCATCCACAATCCTTGT+GGG 0.565259 4.4:-36915159 None:intergenic
AAGATAATCCCTTAAGCCAA+TGG 0.568475 4.4:+36916111 MS.gene23366:CDS
CAAAGTAACATACTAGGAGG+AGG 0.586112 4.4:-36915970 None:intergenic
GAGCATCCAACGAGGACCTG+GGG 0.590570 4.4:+36915088 MS.gene23366:CDS
AACATGCCAAGACTACCTGC+TGG 0.595578 4.4:+36915937 MS.gene23366:CDS
TTAGTGTTCCAATAGTATAC+GGG 0.596441 4.4:+36914873 MS.gene23366:CDS
TAACATACTAGGAGGAGGCA+AGG 0.606191 4.4:-36915965 None:intergenic
AGGTGCATCCACAATCCTTG+TGG 0.606632 4.4:-36915160 None:intergenic
CAAACGACTCACAAGTTCAC+AGG 0.610052 4.4:-36915264 None:intergenic
ATTGAGACTGAGCTTGATCG+TGG 0.614512 4.4:-36913954 None:intergenic
TATACAGATATCATCAGTTG+AGG 0.617329 4.4:+36915785 MS.gene23366:intron
GCCTCTGACAACTCAAAAGG+AGG 0.617900 4.4:-36915180 None:intergenic
CGACAGATAAGTTTGATCGA+TGG 0.622925 4.4:+36918349 MS.gene23366:CDS
ACATGCCAAGACTACCTGCT+GGG 0.629730 4.4:+36915938 MS.gene23366:CDS
AGGTTGTATCCAGATGATGA+AGG 0.632066 4.4:+36915805 MS.gene23366:CDS
ATTGGGTGAGAAATAGGGGT+GGG 0.632597 4.4:-36913985 None:intergenic
GATTGGGTGAGAAATAGGGG+TGG 0.652805 4.4:-36913986 None:intergenic
CTAGTTTCAATAATCCCACA+AGG 0.653695 4.4:+36915145 MS.gene23366:CDS
GATGCTAGTAAACGGAGGAG+AGG 0.657501 4.4:+36916080 MS.gene23366:CDS
ACTGTGGAAATAAAGTGTGA+TGG 0.663057 4.4:-36915226 None:intergenic
AGCATCCAACGAGGACCTGG+GGG 0.700369 4.4:+36915089 MS.gene23366:CDS
TCGAAGATGCTAGTAAACGG+AGG 0.723176 4.4:+36916075 MS.gene23366:CDS
TGTTCGTGGAGCATCCAACG+AGG 0.768144 4.4:+36915080 MS.gene23366:CDS
GCTTGTCGCAAACATCACTG+TGG 0.771336 4.4:-36915242 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAGTAAAAGATAAAATAA+AGG - chr4.4:36914445-36914464 None:intergenic 10.0%
!! TTATCATTATTAATAAAATG+CGG - chr4.4:36915554-36915573 None:intergenic 10.0%
!!! ATTAAAAAGTTAGTTTAATT+AGG + chr4.4:36917094-36917113 MS.gene23366:intron 10.0%
!!! TTAAAAAGTTAGTTTAATTA+GGG + chr4.4:36917095-36917114 MS.gene23366:intron 10.0%
!!! TTTTTCTTAAATAAGATTAT+TGG + chr4.4:36916607-36916626 MS.gene23366:intron 10.0%
!! AATAAATGTATTGCTTAAAT+GGG + chr4.4:36914976-36914995 MS.gene23366:intron 15.0%
!! AATATGCTTAAATAAATTTC+TGG + chr4.4:36916853-36916872 MS.gene23366:intron 15.0%
!! ATCTATTCTAATTAATACAA+AGG - chr4.4:36916578-36916597 None:intergenic 15.0%
!! CAACTTAATAATAAAATACA+AGG - chr4.4:36917836-36917855 None:intergenic 15.0%
!! TAATAAATGTATTGCTTAAA+TGG + chr4.4:36914975-36914994 MS.gene23366:intron 15.0%
!! TTAATTAAAACAACAATTGA+AGG - chr4.4:36914954-36914973 None:intergenic 15.0%
!!! ATATTTTGAATAAAACCATT+TGG - chr4.4:36916672-36916691 None:intergenic 15.0%
!!! CTATTTTATCTTAGTTATTT+AGG + chr4.4:36916948-36916967 MS.gene23366:intron 15.0%
!! AAAGAAAATATTTAGGAAAG+AGG - chr4.4:36917159-36917178 None:intergenic 20.0%
!! AAAGCTTCAAATTTGAATAA+AGG - chr4.4:36914136-36914155 None:intergenic 20.0%
!! AAATTGATGAAAGATAATAG+AGG - chr4.4:36917453-36917472 None:intergenic 20.0%
!! AACAATGTTTATGTTATACA+AGG + chr4.4:36915031-36915050 MS.gene23366:intron 20.0%
!! AACATATAATCAAGAATCTT+CGG + chr4.4:36916452-36916471 MS.gene23366:intron 20.0%
!! AAGTTAATTCTATCATCAAA+AGG + chr4.4:36917936-36917955 MS.gene23366:intron 20.0%
!! AATTTGCACTGTATATTTAA+TGG + chr4.4:36917129-36917148 MS.gene23366:intron 20.0%
!! AGTTAATTCTATCATCAAAA+GGG + chr4.4:36917937-36917956 MS.gene23366:intron 20.0%
!! ATTATCTTTCATCAATTTAC+AGG + chr4.4:36917455-36917474 MS.gene23366:intron 20.0%
!! CAGAAATAAAAAGTAAATCA+TGG - chr4.4:36917729-36917748 None:intergenic 20.0%
!! GAAGAACAAAGAAAATATTT+AGG - chr4.4:36917166-36917185 None:intergenic 20.0%
!! TAAATCATTTAGTTACAAAC+AGG - chr4.4:36917376-36917395 None:intergenic 20.0%
!! TGTTCTAATAATATCTATGT+GGG + chr4.4:36916784-36916803 MS.gene23366:intron 20.0%
!! TTCAAATAAATGTATCATCA+TGG - chr4.4:36916260-36916279 None:intergenic 20.0%
!! TTGTTCTAATAATATCTATG+TGG + chr4.4:36916783-36916802 MS.gene23366:intron 20.0%
!!! ATTTTGACATTCACAAAATA+TGG - chr4.4:36915728-36915747 None:intergenic 20.0%
!!! TTTTACTCAAATAAGATTGT+AGG + chr4.4:36917322-36917341 MS.gene23366:intron 20.0%
! AAATAGTGTTTCATTAATCG+TGG + chr4.4:36918046-36918065 MS.gene23366:intron 25.0%
! AACAATTTACAGCAAGTATA+TGG + chr4.4:36916288-36916307 MS.gene23366:intron 25.0%
! AAGATGAAATAACTATCAGT+TGG - chr4.4:36918289-36918308 None:intergenic 25.0%
! AAGTACCATATGTAAAAGTA+AGG - chr4.4:36917019-36917038 None:intergenic 25.0%
! AATAAGCTAAAAACATCCAA+AGG - chr4.4:36914369-36914388 None:intergenic 25.0%
! AATGAATAACTTTCAAGCAT+TGG - chr4.4:36915647-36915666 None:intergenic 25.0%
! ACATCCATTATTATCAATTG+TGG + chr4.4:36917583-36917602 MS.gene23366:intron 25.0%
! ACGTTAATTAAACTCAAATG+TGG - chr4.4:36916029-36916048 None:intergenic 25.0%
! ATTAGTGTTCCAATAGTATA+CGG + chr4.4:36914872-36914891 MS.gene23366:CDS 25.0%
! CAATTCAATCAAGTCAATAA+GGG - chr4.4:36914591-36914610 None:intergenic 25.0%
! CATCCATTATTATCAATTGT+GGG + chr4.4:36917584-36917603 MS.gene23366:intron 25.0%
! CCATTATCATCAATATATTG+TGG + chr4.4:36916754-36916773 MS.gene23366:intron 25.0%
! GTTAATGATATGTAACAAAC+AGG - chr4.4:36915437-36915456 None:intergenic 25.0%
! GTTCTAATAATATCTATGTG+GGG + chr4.4:36916785-36916804 MS.gene23366:intron 25.0%
! TCAACGAATAATACAAAAGT+GGG - chr4.4:36916540-36916559 None:intergenic 25.0%
! TCAATTCAATCAAGTCAATA+AGG - chr4.4:36914592-36914611 None:intergenic 25.0%
! TCATCAATATATTGTGGATT+AGG + chr4.4:36916760-36916779 MS.gene23366:intron 25.0%
! TGATTATATGTTTAGAAGTG+TGG - chr4.4:36916444-36916463 None:intergenic 25.0%
! TGTTCTAATAATTTCTATGC+CGG + chr4.4:36917614-36917633 MS.gene23366:intron 25.0%
! TTATTTGAGCTTATCTATTG+AGG + chr4.4:36914618-36914637 MS.gene23366:intron 25.0%
! TTGTGATTGTTCATCATTTA+AGG + chr4.4:36916716-36916735 MS.gene23366:intron 25.0%
!! ATCATTTAAGGATTTGTTTC+TGG + chr4.4:36916728-36916747 MS.gene23366:intron 25.0%
!! CCACAATATATTGATGATAA+TGG - chr4.4:36916757-36916776 None:intergenic 25.0%
!! TGTATGTTTTCAAAAATCAC+AGG + chr4.4:36917989-36918008 MS.gene23366:intron 25.0%
!!! CTTAAATAAATTTCTGGTCT+TGG + chr4.4:36916859-36916878 MS.gene23366:intron 25.0%
!!! GAAGTACACAATTTTTTGTA+AGG - chr4.4:36916909-36916928 None:intergenic 25.0%
!!! GTTGTAAAAAGCGTTATTTT+GGG - chr4.4:36914079-36914098 None:intergenic 25.0%
!!! GTTGTTTTCATAAACTATTC+AGG - chr4.4:36914404-36914423 None:intergenic 25.0%
!!! GTTGTTTTTGATTTAGTCTT+TGG + chr4.4:36915289-36915308 MS.gene23366:intron 25.0%
!!! TAGTTATTTCATCTTTTCCA+TGG + chr4.4:36918294-36918313 MS.gene23366:intron 25.0%
AACAGTGAATAATAGACCTA+TGG - chr4.4:36915684-36915703 None:intergenic 30.0%
AACTCTACTAATCCCAAAAT+TGG - chr4.4:36917271-36917290 None:intergenic 30.0%
AAGCATTTGTGATACTCTTT+GGG + chr4.4:36914336-36914355 MS.gene23366:intron 30.0%
ATGTACAATCGACATTTCTA+GGG - chr4.4:36916375-36916394 None:intergenic 30.0%
ATTTCTCACCCAATCAAAAT+GGG + chr4.4:36913994-36914013 MS.gene23366:CDS 30.0%
CAATTGCAAAGTAACATACT+AGG - chr4.4:36915979-36915998 None:intergenic 30.0%
CCATGATAGCTTAATGAAAA+TGG + chr4.4:36914725-36914744 MS.gene23366:intron 30.0%
GGTTAAACTACTTAGATACA+AGG + chr4.4:36917966-36917985 MS.gene23366:intron 30.0%
GTCAACGAATAATACAAAAG+TGG - chr4.4:36916541-36916560 None:intergenic 30.0%
GTTAGATTGAGTTAGTAGTT+TGG + chr4.4:36915516-36915535 MS.gene23366:intron 30.0%
GTTCTAATAATTTCTATGCC+GGG + chr4.4:36917615-36917634 MS.gene23366:intron 30.0%
TAAGCATTTGTGATACTCTT+TGG + chr4.4:36914335-36914354 MS.gene23366:intron 30.0%
TACAGACTTCATTATGAGTA+AGG - chr4.4:36916320-36916339 None:intergenic 30.0%
TACCATATGTAAAAGTAAGG+TGG - chr4.4:36917016-36917035 None:intergenic 30.0%
TACCTTCTTATCATTGTCAT+CGG - chr4.4:36914025-36914044 None:intergenic 30.0%
TATACAGATATCATCAGTTG+AGG + chr4.4:36915785-36915804 MS.gene23366:intron 30.0%
TATTTCTCACCCAATCAAAA+TGG + chr4.4:36913993-36914012 MS.gene23366:CDS 30.0%
TCAAAGTATGCTCAAAAGTA+GGG + chr4.4:36915387-36915406 MS.gene23366:intron 30.0%
TCATAAAATTCCACAACAAC+AGG - chr4.4:36915853-36915872 None:intergenic 30.0%
TCATGGAAACAGTATATCTT+TGG - chr4.4:36917712-36917731 None:intergenic 30.0%
TTAGTGTTCCAATAGTATAC+GGG + chr4.4:36914873-36914892 MS.gene23366:CDS 30.0%
TTCAAAGTATGCTCAAAAGT+AGG + chr4.4:36915386-36915405 MS.gene23366:intron 30.0%
TTCACACAAAAAATGTGCTA+AGG + chr4.4:36915007-36915026 MS.gene23366:intron 30.0%
TTGATATACTACATGACCAT+AGG + chr4.4:36915665-36915684 MS.gene23366:intron 30.0%
TTTATGCACTCCGAAAAAAA+GGG - chr4.4:36914106-36914125 None:intergenic 30.0%
! AACTACTTTGTTTACCCAAA+TGG - chr4.4:36917887-36917906 None:intergenic 30.0%
! CAAGGTATCAATCACATAAA+TGG + chr4.4:36915049-36915068 MS.gene23366:intron 30.0%
! CCATTTTCATTAAGCTATCA+TGG - chr4.4:36914728-36914747 None:intergenic 30.0%
! CTATTGAGGTAAACACTTTT+GGG + chr4.4:36914632-36914651 MS.gene23366:intron 30.0%
! TCTATTGAGGTAAACACTTT+TGG + chr4.4:36914631-36914650 MS.gene23366:intron 30.0%
! TGACCCACAATTGATAATAA+TGG - chr4.4:36917590-36917609 None:intergenic 30.0%
! TGTTGTGGAATTTTATGATG+AGG + chr4.4:36915855-36915874 MS.gene23366:CDS 30.0%
!! AAATGGACTATATATGTTCG+TGG + chr4.4:36915066-36915085 MS.gene23366:CDS 30.0%
!! TTCCACCTTACTTTTACATA+TGG + chr4.4:36917011-36917030 MS.gene23366:intron 30.0%
!! TTTTGATTGGGTGAGAAATA+GGG - chr4.4:36913993-36914012 None:intergenic 30.0%
!!! ACTTTATTTTCCCCATAAAC+AGG - chr4.4:36915318-36915337 None:intergenic 30.0%
!!! ATTTTGATTGGGTGAGAAAT+AGG - chr4.4:36913994-36914013 None:intergenic 30.0%
!!! CCTTTGTTTTCATTCAGATT+CGG + chr4.4:36914046-36914065 MS.gene23366:intron 30.0%
!!! GCTTCAACTCTATTTTTGTT+TGG + chr4.4:36917232-36917251 MS.gene23366:intron 30.0%
!!! GGTTGTAAAAAGCGTTATTT+TGG - chr4.4:36914080-36914099 None:intergenic 30.0%
!!! TACAACCATACCCTTTTTTT+CGG + chr4.4:36914093-36914112 MS.gene23366:intron 30.0%
!!! TTAGCACATTTTTTGTGTGA+AGG - chr4.4:36915008-36915027 None:intergenic 30.0%
AAGATAATCCCTTAAGCCAA+TGG + chr4.4:36916111-36916130 MS.gene23366:CDS 35.0%
ACTGAACATGGTCAATGATT+TGG - chr4.4:36917785-36917804 None:intergenic 35.0%
ACTGTGGAAATAAAGTGTGA+TGG - chr4.4:36915229-36915248 None:intergenic 35.0%
AGAGTTTAGCATACTGAACA+TGG - chr4.4:36917797-36917816 None:intergenic 35.0%
AGATCACAGTATCACACAAA+AGG - chr4.4:36915348-36915367 None:intergenic 35.0%
ATCATACATGTACACGTGTT+TGG + chr4.4:36916339-36916358 MS.gene23366:intron 35.0%
ATCCGATGACAATGATAAGA+AGG + chr4.4:36914020-36914039 MS.gene23366:CDS 35.0%
ATCCTTTAATGAACAAGCAC+AGG - chr4.4:36917554-36917573 None:intergenic 35.0%
CAATCGAAGATGCTAGTAAA+CGG + chr4.4:36916072-36916091 MS.gene23366:CDS 35.0%
CAATCTAACTGAACTTAGCA+GGG - chr4.4:36915505-36915524 None:intergenic 35.0%
CATGTACAATCGACATTTCT+AGG - chr4.4:36916376-36916395 None:intergenic 35.0%
CCGAATCTGAATGAAAACAA+AGG - chr4.4:36914049-36914068 None:intergenic 35.0%
CTAGTTTCAATAATCCCACA+AGG + chr4.4:36915145-36915164 MS.gene23366:CDS 35.0%
CTATCATCAAAAGGGAAATG+TGG + chr4.4:36917945-36917964 MS.gene23366:intron 35.0%
CTTTATGCACTCCGAAAAAA+AGG - chr4.4:36914107-36914126 None:intergenic 35.0%
GATCACAGTATCACACAAAA+GGG - chr4.4:36915347-36915366 None:intergenic 35.0%
TAAAAAAGCTTCAGAGGGAT+CGG - chr4.4:36915890-36915909 None:intergenic 35.0%
TATTACTCTGTGTAGAACTC+AGG + chr4.4:36914827-36914846 MS.gene23366:intron 35.0%
TCAATCTAACTGAACTTAGC+AGG - chr4.4:36915506-36915525 None:intergenic 35.0%
TGATGAACAATCACAAGAGA+TGG - chr4.4:36916712-36916731 None:intergenic 35.0%
TGTATCAACTTCCACAAATG+AGG - chr4.4:36918167-36918186 None:intergenic 35.0%
TTACTCTTTCTTGACGACTT+TGG + chr4.4:36918224-36918243 MS.gene23366:intron 35.0%
TTGCAAAGTAACATACTAGG+AGG - chr4.4:36915976-36915995 None:intergenic 35.0%
TTTGATTGGGTGAGAAATAG+GGG - chr4.4:36913992-36914011 None:intergenic 35.0%
! AGCATTTTGAATATTCAGCC+CGG - chr4.4:36917636-36917655 None:intergenic 35.0%
! ATGATGCTTACGTCCAATTT+TGG + chr4.4:36917255-36917274 MS.gene23366:intron 35.0%
! GATTCTTGTTTGTGACCAAA+TGG + chr4.4:36916654-36916673 MS.gene23366:intron 35.0%
! TGATGCTTACGTCCAATTTT+GGG + chr4.4:36917256-36917275 MS.gene23366:intron 35.0%
!!! AGGTAAACACTTTTGGGTTT+GGG + chr4.4:36914638-36914657 MS.gene23366:intron 35.0%
AAAAAAGCTTCAGAGGGATC+GGG - chr4.4:36915889-36915908 None:intergenic 40.0%
AACAGGCTTCTTTGTGGAAA+AGG - chr4.4:36915836-36915855 None:intergenic 40.0%
ACGAGCTAAAAAAGCTTCAG+AGG - chr4.4:36915896-36915915 None:intergenic 40.0%
AGGTTGTATCCAGATGATGA+AGG + chr4.4:36915805-36915824 MS.gene23366:CDS 40.0%
AGTCTTTGGTTGCCTGTTTA+TGG + chr4.4:36915303-36915322 MS.gene23366:intron 40.0%
CAAAGTAACATACTAGGAGG+AGG - chr4.4:36915973-36915992 None:intergenic 40.0%
CAAGAATCCTTTACATGCAC+AGG - chr4.4:36916642-36916661 None:intergenic 40.0%
CAAGCTCAGTCTCAATTAAG+TGG + chr4.4:36913961-36913980 MS.gene23366:CDS 40.0%
CAATAATCCCACAAGGATTG+TGG + chr4.4:36915152-36915171 MS.gene23366:CDS 40.0%
CACCTGTGCTTGTTCATTAA+AGG + chr4.4:36917549-36917568 MS.gene23366:intron 40.0%
CGACAGATAAGTTTGATCGA+TGG + chr4.4:36918349-36918368 MS.gene23366:CDS 40.0%
CGAGCTAAAAAAGCTTCAGA+GGG - chr4.4:36915895-36915914 None:intergenic 40.0%
CGTTTACTAGCATCTTCGAT+TGG - chr4.4:36916074-36916093 None:intergenic 40.0%
CTGAGAAATTCAGGAACCAT+TGG - chr4.4:36916130-36916149 None:intergenic 40.0%
GTAGTTACCTGTGCATGTAA+AGG + chr4.4:36916632-36916651 MS.gene23366:intron 40.0%
GTATGCTAAACTCTGTGTGA+TGG + chr4.4:36917803-36917822 MS.gene23366:intron 40.0%
GTCTTTGGTTGCCTGTTTAT+GGG + chr4.4:36915304-36915323 MS.gene23366:intron 40.0%
TCTGTGATCATGCTCTATAG+AGG + chr4.4:36917745-36917764 MS.gene23366:intron 40.0%
TCTTTGGTTGCCTGTTTATG+GGG + chr4.4:36915305-36915324 MS.gene23366:intron 40.0%
TGCTTATGTGTCCTCATTTG+TGG + chr4.4:36918153-36918172 MS.gene23366:intron 40.0%
TGTAAAGTCTTGCAGTACTG+TGG + chr4.4:36915472-36915491 MS.gene23366:intron 40.0%
! CATCTGCTTCTGAGAAATTC+AGG - chr4.4:36916139-36916158 None:intergenic 40.0%
! CTCTTTGGGAAGATTTCCTT+TGG + chr4.4:36914350-36914369 MS.gene23366:intron 40.0%
! TATACGGGAACGTAGCATTT+TGG + chr4.4:36914888-36914907 MS.gene23366:CDS 40.0%
! TTTAGGATCCCTCCATTACA+TGG + chr4.4:36916965-36916984 MS.gene23366:intron 40.0%
! TTTCATCTTTTCCATGGCGT+AGG + chr4.4:36918300-36918319 MS.gene23366:intron 40.0%
!! ATCGTGGTTTTTCGAAGATG+AGG - chr4.4:36913941-36913960 None:intergenic 40.0%
!! ATTCAGGAACCATTGGCTTA+AGG - chr4.4:36916123-36916142 None:intergenic 40.0%
!! TCGGATTTGCCCATTTTGAT+TGG - chr4.4:36914006-36914025 None:intergenic 40.0%
!! TTCAGGAACCATTGGCTTAA+GGG - chr4.4:36916122-36916141 None:intergenic 40.0%
!!! CGGATTTGCCCATTTTGATT+GGG - chr4.4:36914005-36914024 None:intergenic 40.0%
!!! GAGGTAAACACTTTTGGGTT+TGG + chr4.4:36914637-36914656 MS.gene23366:intron 40.0%
AAAAAGCTTCAGAGGGATCG+GGG - chr4.4:36915888-36915907 None:intergenic 45.0%
AAGGAACTGCCTTCATCATC+TGG - chr4.4:36915817-36915836 None:intergenic 45.0%
AGTCTTGGCATGTTCACAAC+TGG - chr4.4:36915931-36915950 None:intergenic 45.0%
ATTGAGACTGAGCTTGATCG+TGG - chr4.4:36913957-36913976 None:intergenic 45.0%
ATTGGGTGAGAAATAGGGGT+GGG - chr4.4:36913988-36914007 None:intergenic 45.0%
CAAACGACTCACAAGTTCAC+AGG - chr4.4:36915267-36915286 None:intergenic 45.0%
CACAAAGAAGCCTGTTGTTG+TGG + chr4.4:36915840-36915859 MS.gene23366:CDS 45.0%
CACAACAACAGGCTTCTTTG+TGG - chr4.4:36915842-36915861 None:intergenic 45.0%
GATCTCTTGCTTCAGTGTAG+AGG + chr4.4:36915363-36915382 MS.gene23366:intron 45.0%
GCACTCCGAAAAAAAGGGTA+TGG - chr4.4:36914101-36914120 None:intergenic 45.0%
GCTACGTTCCCGTATACTAT+TGG - chr4.4:36914884-36914903 None:intergenic 45.0%
GTCTTGGCATGTTCACAACT+GGG - chr4.4:36915930-36915949 None:intergenic 45.0%
TAACATACTAGGAGGAGGCA+AGG - chr4.4:36915968-36915987 None:intergenic 45.0%
TCGAAGATGCTAGTAAACGG+AGG + chr4.4:36916075-36916094 MS.gene23366:CDS 45.0%
TGTGGAAAAATGAAAGCCGC+AGG - chr4.4:36916011-36916030 None:intergenic 45.0%
! GGCAACACAGTTTTCTCACA+AGG - chr4.4:36918268-36918287 None:intergenic 45.0%
! TTTGGGTTTGGGAGAACTGA+TGG + chr4.4:36914649-36914668 MS.gene23366:intron 45.0%
!! CAAGTTGATTGCTCGAGTTG+TGG + chr4.4:36917860-36917879 MS.gene23366:intron 45.0%
!!! ACCTCCTTTTGAGTTGTCAG+AGG + chr4.4:36915179-36915198 MS.gene23366:CDS 45.0%
!!! TTTAAAATATATAATATTTG+TGG + chr4.4:36916213-36916232 MS.gene23366:intron 5.0%
AACATGCCAAGACTACCTGC+TGG + chr4.4:36915937-36915956 MS.gene23366:CDS 50.0%
ACATGCCAAGACTACCTGCT+GGG + chr4.4:36915938-36915957 MS.gene23366:CDS 50.0%
AGCAGATGAACTCTTGCAGC+TGG + chr4.4:36916151-36916170 MS.gene23366:CDS 50.0%
AGGTGCATCCACAATCCTTG+TGG - chr4.4:36915163-36915182 None:intergenic 50.0%
ATGAAAGCCGCAGGTGACTA+TGG - chr4.4:36916002-36916021 None:intergenic 50.0%
ATGAGCTTGAACCTACGCCA+TGG - chr4.4:36918314-36918333 None:intergenic 50.0%
CACGCTTTACAATAACCCCC+AGG - chr4.4:36915107-36915126 None:intergenic 50.0%
CAGGAATTCCTCTCATGCAC+AGG - chr4.4:36917357-36917376 None:intergenic 50.0%
CCTGCCTCTGACAACTCAAA+AGG - chr4.4:36915186-36915205 None:intergenic 50.0%
GATCCCTCCATTACATGGTG+AGG + chr4.4:36916970-36916989 MS.gene23366:intron 50.0%
GATGCTAGTAAACGGAGGAG+AGG + chr4.4:36916080-36916099 MS.gene23366:CDS 50.0%
GATTGGGTGAGAAATAGGGG+TGG - chr4.4:36913989-36914008 None:intergenic 50.0%
GCAATTGCCATAGTCACCTG+CGG + chr4.4:36915992-36916011 MS.gene23366:intron 50.0%
GCCTCTGACAACTCAAAAGG+AGG - chr4.4:36915183-36915202 None:intergenic 50.0%
GCTTGTCGCAAACATCACTG+TGG - chr4.4:36915245-36915264 None:intergenic 50.0%
GGTGCATCCACAATCCTTGT+GGG - chr4.4:36915162-36915181 None:intergenic 50.0%
TCAGCCACCTCACCATGTAA+TGG - chr4.4:36916980-36916999 None:intergenic 50.0%
TGAGTTGTCAGAGGCAGGAT+GGG + chr4.4:36915188-36915207 MS.gene23366:CDS 50.0%
! CCACCTCACCATGTAATGGA+GGG - chr4.4:36916976-36916995 None:intergenic 50.0%
! GCTCGAGTTGTGGTCCATTT+GGG + chr4.4:36917870-36917889 MS.gene23366:intron 50.0%
! TGCTCGAGTTGTGGTCCATT+TGG + chr4.4:36917869-36917888 MS.gene23366:intron 50.0%
! TGTACTGACCTGTGCATGAG+AGG + chr4.4:36917346-36917365 MS.gene23366:intron 50.0%
! TTGAGTTGTCAGAGGCAGGA+TGG + chr4.4:36915187-36915206 MS.gene23366:CDS 50.0%
!!! CCTTTTGAGTTGTCAGAGGC+AGG + chr4.4:36915183-36915202 MS.gene23366:CDS 50.0%
!!! GGGAACGTAGCATTTTGGCT+TGG + chr4.4:36914893-36914912 MS.gene23366:CDS 50.0%
AATAACCCCCAGGTCCTCGT+TGG - chr4.4:36915097-36915116 None:intergenic 55.0%
CAGCTCGTCAGCAGGTATGA+TGG + chr4.4:36916177-36916196 MS.gene23366:intron 55.0%
CCCTCCATTACATGGTGAGG+TGG + chr4.4:36916973-36916992 MS.gene23366:intron 55.0%
GAGTTGTCAGAGGCAGGATG+GGG + chr4.4:36915189-36915208 MS.gene23366:CDS 55.0%
GCCACCTCACCATGTAATGG+AGG - chr4.4:36916977-36916996 None:intergenic 55.0%
GTAAGCCCAGCAGGTAGTCT+TGG - chr4.4:36915946-36915965 None:intergenic 55.0%
! TGTTCGTGGAGCATCCAACG+AGG + chr4.4:36915080-36915099 MS.gene23366:CDS 55.0%
AGCATCCAACGAGGACCTGG+GGG + chr4.4:36915089-36915108 MS.gene23366:CDS 60.0%
GAGCATCCAACGAGGACCTG+GGG + chr4.4:36915088-36915107 MS.gene23366:CDS 60.0%
! GGAGCATCCAACGAGGACCT+GGG + chr4.4:36915087-36915106 MS.gene23366:CDS 60.0%
! TGGAGCATCCAACGAGGACC+TGG + chr4.4:36915086-36915105 MS.gene23366:CDS 60.0%
GGAGGCAAGGTAAGCCCAGC+AGG - chr4.4:36915955-36915974 None:intergenic 65.0%
GCTGGCAGCAGCTCGTCAGC+AGG + chr4.4:36916169-36916188 MS.gene23366:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 36913928 36918408 36913928 ID=MS.gene23366
chr4.4 mRNA 36913928 36918408 36913928 ID=MS.gene23366.t1;Parent=MS.gene23366
chr4.4 exon 36913928 36914041 36913928 ID=MS.gene23366.t1.exon1;Parent=MS.gene23366.t1
chr4.4 CDS 36913928 36914041 36913928 ID=cds.MS.gene23366.t1;Parent=MS.gene23366.t1
chr4.4 exon 36914842 36914930 36914842 ID=MS.gene23366.t1.exon2;Parent=MS.gene23366.t1
chr4.4 CDS 36914842 36914930 36914842 ID=cds.MS.gene23366.t1;Parent=MS.gene23366.t1
chr4.4 exon 36915053 36915274 36915053 ID=MS.gene23366.t1.exon3;Parent=MS.gene23366.t1
chr4.4 CDS 36915053 36915274 36915053 ID=cds.MS.gene23366.t1;Parent=MS.gene23366.t1
chr4.4 exon 36915792 36915979 36915792 ID=MS.gene23366.t1.exon4;Parent=MS.gene23366.t1
chr4.4 CDS 36915792 36915979 36915792 ID=cds.MS.gene23366.t1;Parent=MS.gene23366.t1
chr4.4 exon 36916069 36916190 36916069 ID=MS.gene23366.t1.exon5;Parent=MS.gene23366.t1
chr4.4 CDS 36916069 36916190 36916069 ID=cds.MS.gene23366.t1;Parent=MS.gene23366.t1
chr4.4 exon 36918322 36918408 36918322 ID=MS.gene23366.t1.exon6;Parent=MS.gene23366.t1
chr4.4 CDS 36918322 36918408 36918322 ID=cds.MS.gene23366.t1;Parent=MS.gene23366.t1
Gene Sequence

>MS.gene23366

ATGCTCAACACCTCATCTTCGAAAAACCACGATCAAGCTCAGTCTCAATTAAGTGGTCCCACCCCTATTTCTCACCCAATCAAAATGGGCAAATCCGATGACAATGATAAGAAGGTAACCTTTGTTTTCATTCAGATTCGGTTAAAATCCCAAAATAACGCTTTTTACAACCATACCCTTTTTTTCGGAGTGCATAAAGTTTGCACCTTTATTCAAATTTGAAGCTTTGATTTGATACAAACTTATCACACTGCAATAACTTGAAATCAATCTGTAGAATGTTAAATTTGAAAATGATTTTTGAATGTGAATGCAATGTGAAGAGAAATTTTTCTATTTACTGAAGTAGAATCTCTTATTGATAATGAGCTTGATTGAATTGAATCAGTTAAGCTTATTTACTGATATAAGCATTTGTGATACTCTTTGGGAAGATTTCCTTTGGATGTTTTTAGCTTATTTCAATAAGCTTTCCTGAATAGTTTATGAAAACAACATATATGCAAATAGTTTGCCTTTATTTTATCTTTTACTATAGAAATAGTTTAAGAATAAGCACTTACATTACTATAAGCGTTAAATTAATTTGTTTATTCGAACATGTCGTATATTATCTGAGCTTGATTTAATTGTTCAAATGAGTTTTTGATCTTGTGTCGACCCTTATTGACTTGATTGAATTGAATTGAATTATTTGAGCTTATCTATTGAGGTAAACACTTTTGGGTTTGGGAGAACTGATGGAAACAACTTATAACATTTTCTTAAGCTATTTTCAGCTTATTTATATAAGTTTTCCATGATAGCTTAATGAAAATGGCTTATAGTTTGTAGCTTGTATGAAAACAATTTGTCTTTATTTTATCTTTTGTTACATAAAAAGCTTAATTAAGTGCTTTTATTACTCTGTGTAGAACTCAGGTAAGAAACTCAAAGATGTAGAAATTAGTGTTCCAATAGTATACGGGAACGTAGCATTTTGGCTTGGCAAAAAAGCTAGTGAGTAAGTAACTTACATTGCATCCTTCAATTGTTGTTTTAATTAATTAATAAATGTATTGCTTAAATGGGTTTTTTCCTTCACACAAAAAATGTGCTAAGGTAACAATGTTTATGTTATACAAGGTATCAATCACATAAATGGACTATATATGTTCGTGGAGCATCCAACGAGGACCTGGGGGTTATTGTAAAGCGTGTTGTTTTTCAGTTGCATACTAGTTTCAATAATCCCACAAGGATTGTGGATGCACCTCCTTTTGAGTTGTCAGAGGCAGGATGGGGAGAATTTGAAATTGCCATCACACTTTATTTCCACAGTGATGTTTGCGACAAGCCTGTGAACTTGTGAGTCGTTTGATGTTGTTTTTGATTTAGTCTTTGGTTGCCTGTTTATGGGGAAAATAAAGTTTCTTACCCTTTTGTGTGATACTGTGATCTCTTGCTTCAGTGTAGAGGTTCAAAGTATGCTCAAAAGTAGGGAAAATCTCAAATAGTGTTTTAAGTCCTGTTTGTTACATATCATTAACTATGTGCTTTTGTTTTGTAAAGTCTTGCAGTACTGTGGCTCTATGCCCTGCTAAGTTCAGTTAGATTGAGTTAGTAGTTTGGTTTGCTTTTGTGCCGCATTTTATTAATAATGATAAGCTTAAATGATTATAAAACAACTAATATCTATCGTGTTATGTTTTTATTAGAGCATGCAGCTATACGATTCCAATGCTTGAAAGTTATTCATTGATATACTACATGACCATAGGTCTATTATTCACTGTTATAAATCATGAAGATTGCAAACCATATTTTGTGAATGTCAAAATAATTTCTGAATCTTGTTTTTTATTGAAGTGTAATGTATATACAGATATCATCAGTTGAGGTTGTATCCAGATGATGAAGGCAGTTCCTTTTCCACAAAGAAGCCTGTTGTTGTGGAATTTTATGATGAGGTAGTTTTCCCCGATCCCTCTGAAGCTTTTTTAGCTCGTGTGCAGAATCACCCAGTTGTGAACATGCCAAGACTACCTGCTGGGCTTACCTTGCCTCCTCCTAGTATGTTACTTTGCAATTGCCATAGTCACCTGCGGCTTTCATTTTTCCACATTTGAGTTTAATTAACGTTACATTATCTATAAATGCAGTTCCAATCGAAGATGCTAGTAAACGGAGGAGAGGTGATACTAAAGATAATCCCTTAAGCCAATGGTTCCTGAATTTCTCAGAAGCAGATGAACTCTTGCAGCTGGCAGCAGCTCGTCAGCAGGTATGATGGACATAATATCATATTTAAAATATATAATATTTGTGGAAGTCGAAGTAGTTGTTGTTGCCATGATGATACATTTATTTGAATTTGAATCAACAATTTACAGCAAGTATATGGTTGCATCCTTACTCATAATGAAGTCTGTATCATACATGTACACGTGTTTGGCTGTTTATTACCCTAGAAATGTCGATTGTACATGAGTGTGATAGCATCAAGTTTTATGATTTTGTTCTAAGTATTCTCTCCACACTTCTAAACATATAATCAAGAATCTTCGGTTAAAGATTCAAAACTTCTGTTCTATACTTGAAGTTTATTTTTGTTTACGTGATGCTTATGACCCACTTTTGTATTATTCGTTGACTCAAAATGATTATGCCTTTGTATTAATTAGAATAGATTGCGTCTCATTTTTCTTAAATAAGATTATTGGTTGTAGTTACCTGTGCATGTAAAGGATTCTTGTTTGTGACCAAATGGTTTTATTCAAAATATTTCATAGTTATCTTGTACCATCTCTTGTGATTGTTCATCATTTAAGGATTTGTTTCTGGCATCCATTATCATCAATATATTGTGGATTAGGTTGTTCTAATAATATCTATGTGGGGCATGAATGTTCAGAGTGTATATCATAATGCTATTTTATACTAATAAATATGCTTAAATAAATTTCTGGTCTTGGTAATAGAAGCTTTTCAGCTTTGTTCCTTACAAAAAATTGTGTACTTCTATATGAAGAAAGAAAAATCTATTTTATCTTAGTTATTTAGGATCCCTCCATTACATGGTGAGGTGGCTGACAGTGACATGATTCCACCTTACTTTTACATATGGTACTTATTTGTTCTTTATTTAAAATTTATATAATATTTGTATAAATAAACATAAATAGAAATTAAAAAGTTAGTTTAATTAGGGTTAAAAGTTAGAATTTGCACTGTATATTTAATGGAGAACCTCTTTCCTAAATATTTTCTTTGTTCTTCGTTCATAACATATAATGATGACTTATCAGTCAAAACTTTTTTCTATGCTTCAACTCTATTTTTGTTTGGATGATGCTTACGTCCAATTTTGGGATTAGTAGAGTTAAAATAATTGTCTTCGAATTATTAGTACAAATTTTACTCAAATAAGATTGTAGGTTGTACTGACCTGTGCATGAGAGGAATTCCTGTTTGTAACTAAATGATTTAATTCAACTTCTAAAGAAAGAGCGTGTTCTTCATTGAAGAGTTTGCTTCTGTCATCCTCTATTATCTTTCATCAATTTACAGGTCAGAGAGAAAAGACATTGTACATGCAAATTAACATTATTTCAATTTTATGTAATAGCTATCTCGTACTATCACCTGTGCTTGTTCATTAAAGGATTTGCTTCTGACATCCATTATTATCAATTGTGGGTCATGTGTGTTCTAATAATTTCTATGCCGGGCTGAATATTCAAAATGCTTATCATAATGCTCTAAATAATAAATACTAGTAAGTCATTCTATGTCATTAAGACCAAAGATATACTGTTTCCATGATTTACTTTTTATTTCTGTGATCATGCTCTATAGAGGAACTTATTTTTTCTCCAAATCATTGACCATGTTCAGTATGCTAAACTCTGTGTGATGGTTCTTTTCCTTGTATTTTATTATTAAGTTGTTGTCAAGTTGATTGCTCGAGTTGTGGTCCATTTGGGTAAACAAAGTAGTTAAGTGCTTATCATATAAGCGTTTATGAATAAGTTAATTCTATCATCAAAAGGGAAATGTGGTTAAACTACTTAGATACAAGGTGTATGTTTTCAAAAATCACAGGCTATTGTTTATAATTTACTGAAACAAGTCGACGAAAATAGTGTTTCATTAATCGTGGACTAGTCATAAACTGTTTCATGAGTTAACTCAAACTACACGAGTGCTTATGTTATAAGATAAGCTTAAATAAGCTAATTATTGATGCTTATGTGTCCTCATTTGTGGAAGTTGATACATGCTGAATAGTACTTGTTGCACAAATTATAATATGCTTTACTCTTTCTTGACGACTTTGGATGACTCTATCATCTTAACCTTGTGAGAAAACTGTGTTGCCAACTGATAGTTATTTCATCTTTTCCATGGCGTAGGTTCAAGCTCATATTTCAAAACTCAAACGACAGATAAGTTTGATCGATGGACAGCATAAACAGCTTAAATCTTCTCCTGACCAATAG
Protein sequence

>MS.gene23366.t1

MLNTSSSKNHDQAQSQLSGPTPISHPIKMGKSDDNDKKNSGKKLKDVEISVPIVYGNVAFWLGKKASEYQSHKWTIYVRGASNEDLGVIVKRVVFQLHTSFNNPTRIVDAPPFELSEAGWGEFEIAITLYFHSDVCDKPVNLYHQLRLYPDDEGSSFSTKKPVVVEFYDEVVFPDPSEAFLARVQNHPVVNMPRLPAGLTLPPPIPIEDASKRRRGDTKDNPLSQWFLNFSEADELLQLAAARQQVQAHISKLKRQISLIDGQHKQLKSSPDQ