Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017137.t1 | XP_003617458.1 | 98.7 | 157 | 2 | 0 | 1 | 157 | 1 | 157 | 2.60E-84 | 321.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017137.t1 | Q682H0 | 63.3 | 158 | 57 | 1 | 1 | 157 | 1 | 158 | 2.0e-53 | 209.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017137.t1 | B7FNC9 | 98.7 | 157 | 2 | 0 | 1 | 157 | 1 | 157 | 1.9e-84 | 321.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene017137 | MS.gene074024 | PPI |
| MS.gene017137 | MS.gene78507 | PPI |
| MS.gene017137 | MS.gene032837 | PPI |
| MS.gene017137 | MS.gene91904 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017137.t1 | MTR_5g091800 | 98.726 | 157 | 2 | 0 | 1 | 157 | 1 | 157 | 6.96e-116 | 324 |
| MS.gene017137.t1 | MTR_3g452470 | 79.114 | 158 | 32 | 1 | 1 | 157 | 1 | 158 | 2.81e-94 | 270 |
| MS.gene017137.t1 | MTR_4g108350 | 51.333 | 150 | 71 | 1 | 4 | 153 | 12 | 159 | 1.59e-46 | 149 |
| MS.gene017137.t1 | MTR_5g039530 | 44.578 | 166 | 74 | 2 | 2 | 155 | 7 | 166 | 8.60e-41 | 135 |
| MS.gene017137.t1 | MTR_4g108350 | 48.000 | 150 | 67 | 2 | 4 | 153 | 12 | 150 | 2.82e-39 | 130 |
| MS.gene017137.t1 | MTR_3g099010 | 48.760 | 121 | 56 | 1 | 1 | 121 | 1 | 115 | 9.20e-34 | 116 |
| MS.gene017137.t1 | MTR_8g096670 | 50.962 | 104 | 51 | 0 | 1 | 104 | 1 | 104 | 7.52e-33 | 112 |
| MS.gene017137.t1 | MTR_8g096670 | 55.682 | 88 | 39 | 0 | 17 | 104 | 7 | 94 | 5.43e-30 | 105 |
| MS.gene017137.t1 | MTR_4g093260 | 31.333 | 150 | 89 | 2 | 3 | 147 | 6 | 146 | 2.86e-20 | 86.7 |
| MS.gene017137.t1 | MTR_4g093510 | 33.088 | 136 | 77 | 2 | 17 | 147 | 20 | 146 | 2.98e-20 | 86.7 |
| MS.gene017137.t1 | MTR_4g093270 | 31.159 | 138 | 81 | 2 | 17 | 149 | 17 | 145 | 1.16e-19 | 84.0 |
| MS.gene017137.t1 | MTR_4g093260 | 32.540 | 126 | 71 | 2 | 27 | 147 | 21 | 137 | 6.04e-17 | 77.0 |
| MS.gene017137.t1 | MTR_4g093280 | 30.496 | 141 | 84 | 2 | 17 | 152 | 20 | 151 | 2.19e-14 | 69.7 |
| MS.gene017137.t1 | MTR_5g019720 | 29.688 | 128 | 78 | 2 | 7 | 134 | 28 | 143 | 3.57e-14 | 67.0 |
| MS.gene017137.t1 | MTR_5g019720 | 32.990 | 97 | 61 | 1 | 7 | 103 | 28 | 120 | 3.09e-13 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017137.t1 | AT2G42820 | 63.291 | 158 | 57 | 1 | 1 | 157 | 1 | 158 | 2.38e-74 | 219 |
| MS.gene017137.t1 | AT1G74520 | 46.053 | 152 | 80 | 1 | 4 | 155 | 9 | 158 | 4.09e-42 | 138 |
| MS.gene017137.t1 | AT1G74520 | 46.053 | 152 | 80 | 1 | 4 | 155 | 9 | 158 | 4.09e-42 | 138 |
| MS.gene017137.t1 | AT1G69700 | 44.966 | 149 | 79 | 1 | 2 | 147 | 9 | 157 | 4.71e-37 | 125 |
| MS.gene017137.t1 | AT5G50720 | 58.095 | 105 | 44 | 0 | 1 | 105 | 1 | 105 | 2.15e-35 | 119 |
| MS.gene017137.t1 | AT5G62490 | 56.842 | 95 | 41 | 0 | 7 | 101 | 12 | 106 | 1.35e-34 | 119 |
| MS.gene017137.t1 | AT4G24960 | 45.669 | 127 | 68 | 1 | 8 | 134 | 8 | 133 | 4.01e-32 | 112 |
| MS.gene017137.t1 | AT4G24960 | 40.441 | 136 | 71 | 1 | 20 | 155 | 1 | 126 | 1.27e-29 | 105 |
| MS.gene017137.t1 | AT4G24960 | 41.748 | 103 | 59 | 1 | 32 | 134 | 1 | 102 | 7.39e-23 | 87.4 |
| MS.gene017137.t1 | AT4G36720 | 36.842 | 95 | 56 | 1 | 7 | 101 | 36 | 126 | 5.64e-16 | 72.0 |
Find 36 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGACATACGTCAAGAAATT+TGG | 0.280571 | 5.3:+73832848 | MS.gene017137:CDS |
| GTCAAGAAATTTGGTAACTA+TGG | 0.297751 | 5.3:+73832857 | MS.gene017137:CDS |
| ACCAACAGTGGCTAACATAT+TGG | 0.311228 | 5.3:+73832577 | MS.gene017137:CDS |
| GAACGTTATATTGATAGATT+TGG | 0.323087 | 5.3:+73832950 | MS.gene017137:CDS |
| CCAACAGTGGCTAACATATT+GGG | 0.341811 | 5.3:+73832578 | MS.gene017137:CDS |
| CAACTTACAAGGTCCTATAT+TGG | 0.344124 | 5.3:+73832634 | MS.gene017137:CDS |
| ATCACTCTCTCAAATGCATC+TGG | 0.360770 | 5.3:-73832974 | None:intergenic |
| ATATCAATAGGTTTCCCATT+TGG | 0.373951 | 5.3:+73832744 | MS.gene017137:intron |
| AGACATTTGGACACAACAAT+AGG | 0.378599 | 5.3:+73832029 | MS.gene017137:CDS |
| ATTGCTGAACCTACCAATAT+AGG | 0.380907 | 5.3:-73832647 | None:intergenic |
| AAGCTTCATGTATGGCCAAA+TGG | 0.383376 | 5.3:-73832759 | None:intergenic |
| TGGAGCAATACTAAGACATT+TGG | 0.388072 | 5.3:+73832016 | MS.gene017137:CDS |
| CTTCGAGCTTTCAACTTACA+AGG | 0.426910 | 5.3:+73832623 | MS.gene017137:CDS |
| AAAATCATCATGGGTGTCCT+TGG | 0.439409 | 5.3:+73831996 | None:intergenic |
| AGCTTCATGTATGGCCAAAT+GGG | 0.440653 | 5.3:-73832758 | None:intergenic |
| GACATTTGGACACAACAATA+GGG | 0.477953 | 5.3:+73832030 | MS.gene017137:CDS |
| TATTGAAAGTCCTTCAACCT+TGG | 0.507265 | 5.3:+73832551 | MS.gene017137:CDS |
| TGAAGCTTGTGTTCTGCGTA+TGG | 0.521277 | 5.3:+73832774 | MS.gene017137:CDS |
| TGTTGGTCATCCAAGGTTGA+AGG | 0.521506 | 5.3:-73832561 | None:intergenic |
| AGAACCTTCTTGTACTCCTC+AGG | 0.524082 | 5.3:-73832893 | None:intergenic |
| CCCAATATGTTAGCCACTGT+TGG | 0.527231 | 5.3:-73832578 | None:intergenic |
| CAGAACACAAGCTTCATGTA+TGG | 0.542978 | 5.3:-73832767 | None:intergenic |
| TAGCCACTGTTGGTCATCCA+AGG | 0.555283 | 5.3:-73832568 | None:intergenic |
| AGGATAAAGAAGCATTACTC+CGG | 0.560010 | 5.3:-73832423 | None:intergenic |
| TTGGTACTGCCAATGTTCAA+TGG | 0.561469 | 5.3:+73832797 | MS.gene017137:CDS |
| GCTTGTGTTCTGCGTATGGT+TGG | 0.576715 | 5.3:+73832778 | MS.gene017137:CDS |
| GGATAAAGAAGCATTACTCC+GGG | 0.584585 | 5.3:-73832422 | None:intergenic |
| TCATAGATACGCATCAATGA+GGG | 0.589923 | 5.3:+73832527 | MS.gene017137:intron |
| ATCATAGATACGCATCAATG+AGG | 0.601236 | 5.3:+73832526 | MS.gene017137:intron |
| TCACTCTCTCAAATGCATCT+GGG | 0.601880 | 5.3:-73832973 | None:intergenic |
| TAAGCTGCTCCATTGAACAT+TGG | 0.632735 | 5.3:-73832806 | None:intergenic |
| TTATCCTGAGGAGTACAAGA+AGG | 0.638050 | 5.3:+73832889 | MS.gene017137:CDS |
| AATGTCTTAGTATTGCTCCA+AGG | 0.660944 | 5.3:-73832013 | None:intergenic |
| ACATATATGAGAATTATGCA+AGG | 0.691799 | 5.3:+73832828 | MS.gene017137:CDS |
| CAACCTTGGATGACCAACAG+TGG | 0.691907 | 5.3:+73832565 | MS.gene017137:CDS |
| TGGAAATTACAATTATCCTG+AGG | 0.710511 | 5.3:+73832877 | MS.gene017137:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTTAATAAAAAAATATCAAT+AGG | + | chr5.3:73832732-73832751 | MS.gene017137:intron | 10.0% |
| !! | TAAAGATATATTTATAACTT+TGG | - | chr5.3:73833030-73833049 | None:intergenic | 10.0% |
| !!! | TAATTATCTTATCTTTTTAT+TGG | + | chr5.3:73832165-73832184 | MS.gene017137:intron | 10.0% |
| !! | ATAAGATAATTATAGAAAAG+TGG | - | chr5.3:73832157-73832176 | None:intergenic | 15.0% |
| !!! | ACAATTTGATAATATTTATC+TGG | + | chr5.3:73832709-73832728 | MS.gene017137:intron | 15.0% |
| !! | AATATGATCATTACTTACAA+AGG | - | chr5.3:73832446-73832465 | None:intergenic | 20.0% |
| !! | TCTATTTCACTTTATATTTC+AGG | + | chr5.3:73833091-73833110 | MS.gene017137:intron | 20.0% |
| !! | TTCTATAATTATCTAGATGA+AGG | + | chr5.3:73832267-73832286 | MS.gene017137:intron | 20.0% |
| !!! | TTATCTTATCTTTTTATTGG+TGG | + | chr5.3:73832168-73832187 | MS.gene017137:intron | 20.0% |
| ! | AAAAGTAGGATGTTATTAAG+AGG | - | chr5.3:73832390-73832409 | None:intergenic | 25.0% |
| ! | ACATATATGAGAATTATGCA+AGG | + | chr5.3:73832828-73832847 | MS.gene017137:CDS | 25.0% |
| ! | GAAATTCTTTCAAAACACAT+GGG | + | chr5.3:73832328-73832347 | MS.gene017137:intron | 25.0% |
| ! | GAACGTTATATTGATAGATT+TGG | + | chr5.3:73832950-73832969 | MS.gene017137:CDS | 25.0% |
| ATATCAATAGGTTTCCCATT+TGG | + | chr5.3:73832744-73832763 | MS.gene017137:intron | 30.0% | |
| CGAAATTCTTTCAAAACACA+TGG | + | chr5.3:73832327-73832346 | MS.gene017137:intron | 30.0% | |
| GACAATGCATAAAATATGCA+AGG | - | chr5.3:73832216-73832235 | None:intergenic | 30.0% | |
| TGGAAATTACAATTATCCTG+AGG | + | chr5.3:73832877-73832896 | MS.gene017137:CDS | 30.0% | |
| ! | GTCAAGAAATTTGGTAACTA+TGG | + | chr5.3:73832857-73832876 | MS.gene017137:CDS | 30.0% |
| ! | TCTTATCTTTTTATTGGTGG+TGG | + | chr5.3:73832171-73832190 | MS.gene017137:intron | 30.0% |
| !! | TCGTACATGCAATATTTTGA+AGG | - | chr5.3:73832310-73832329 | None:intergenic | 30.0% |
| AATGTCTTAGTATTGCTCCA+AGG | - | chr5.3:73832016-73832035 | None:intergenic | 35.0% | |
| AGACATTTGGACACAACAAT+AGG | + | chr5.3:73832029-73832048 | MS.gene017137:CDS | 35.0% | |
| AGGACATACGTCAAGAAATT+TGG | + | chr5.3:73832848-73832867 | MS.gene017137:CDS | 35.0% | |
| ATCATAGATACGCATCAATG+AGG | + | chr5.3:73832526-73832545 | MS.gene017137:intron | 35.0% | |
| ATGGGACACACTCTAAATTT+AGG | + | chr5.3:73832346-73832365 | MS.gene017137:intron | 35.0% | |
| ATTGCTGAACCTACCAATAT+AGG | - | chr5.3:73832650-73832669 | None:intergenic | 35.0% | |
| CAACTTACAAGGTCCTATAT+TGG | + | chr5.3:73832634-73832653 | MS.gene017137:CDS | 35.0% | |
| GACATTTGGACACAACAATA+GGG | + | chr5.3:73832030-73832049 | MS.gene017137:CDS | 35.0% | |
| TATTGAAAGTCCTTCAACCT+TGG | + | chr5.3:73832551-73832570 | MS.gene017137:CDS | 35.0% | |
| TCATAGATACGCATCAATGA+GGG | + | chr5.3:73832527-73832546 | MS.gene017137:intron | 35.0% | |
| TGCATAAAATATGCAAGGTC+CGG | - | chr5.3:73832211-73832230 | None:intergenic | 35.0% | |
| TGGAGCAATACTAAGACATT+TGG | + | chr5.3:73832016-73832035 | MS.gene017137:CDS | 35.0% | |
| TTACAAGGTCCTATATTGGT+AGG | + | chr5.3:73832638-73832657 | MS.gene017137:intron | 35.0% | |
| !! | AGGATAAAGAAGCATTACTC+CGG | - | chr5.3:73832426-73832445 | None:intergenic | 35.0% |
| !!! | ATCCTACTTTTTTGTGTACC+AGG | + | chr5.3:73832399-73832418 | MS.gene017137:intron | 35.0% |
| AAGCTTCATGTATGGCCAAA+TGG | - | chr5.3:73832762-73832781 | None:intergenic | 40.0% | |
| ACCAACAGTGGCTAACATAT+TGG | + | chr5.3:73832577-73832596 | MS.gene017137:CDS | 40.0% | |
| AGCTTCATGTATGGCCAAAT+GGG | - | chr5.3:73832761-73832780 | None:intergenic | 40.0% | |
| ATCACTCTCTCAAATGCATC+TGG | - | chr5.3:73832977-73832996 | None:intergenic | 40.0% | |
| CAGAACACAAGCTTCATGTA+TGG | - | chr5.3:73832770-73832789 | None:intergenic | 40.0% | |
| CATAAAATATGCAAGGTCCG+GGG | - | chr5.3:73832209-73832228 | None:intergenic | 40.0% | |
| CCAACAGTGGCTAACATATT+GGG | + | chr5.3:73832578-73832597 | MS.gene017137:CDS | 40.0% | |
| CTTCGAGCTTTCAACTTACA+AGG | + | chr5.3:73832623-73832642 | MS.gene017137:CDS | 40.0% | |
| GCATAAAATATGCAAGGTCC+GGG | - | chr5.3:73832210-73832229 | None:intergenic | 40.0% | |
| TAAGCTGCTCCATTGAACAT+TGG | - | chr5.3:73832809-73832828 | None:intergenic | 40.0% | |
| TCACTCTCTCAAATGCATCT+GGG | - | chr5.3:73832976-73832995 | None:intergenic | 40.0% | |
| TTGGTACTGCCAATGTTCAA+TGG | + | chr5.3:73832797-73832816 | MS.gene017137:CDS | 40.0% | |
| ! | TTATCCTGAGGAGTACAAGA+AGG | + | chr5.3:73832889-73832908 | MS.gene017137:CDS | 40.0% |
| !! | GGATAAAGAAGCATTACTCC+GGG | - | chr5.3:73832425-73832444 | None:intergenic | 40.0% |
| !!! | ATCTTTTTATTGGTGGTGGC+CGG | + | chr5.3:73832175-73832194 | MS.gene017137:intron | 40.0% |
| AGAACCTTCTTGTACTCCTC+AGG | - | chr5.3:73832896-73832915 | None:intergenic | 45.0% | |
| CCCAATATGTTAGCCACTGT+TGG | - | chr5.3:73832581-73832600 | None:intergenic | 45.0% | |
| GGCCTGGTACACAAAAAAGT+AGG | - | chr5.3:73832404-73832423 | None:intergenic | 45.0% | |
| TGAAGCTTGTGTTCTGCGTA+TGG | + | chr5.3:73832774-73832793 | MS.gene017137:CDS | 45.0% | |
| TGTTGGTCATCCAAGGTTGA+AGG | - | chr5.3:73832564-73832583 | None:intergenic | 45.0% | |
| ! | TGAGCTTAGCTCAGTTGGTA+TGG | - | chr5.3:73832238-73832257 | None:intergenic | 45.0% |
| !!! | ACTTTTTTGTGTACCAGGCC+CGG | + | chr5.3:73832404-73832423 | MS.gene017137:intron | 45.0% |
| !!! | TCTTTTTATTGGTGGTGGCC+GGG | + | chr5.3:73832176-73832195 | MS.gene017137:intron | 45.0% |
| CAACCTTGGATGACCAACAG+TGG | + | chr5.3:73832565-73832584 | MS.gene017137:CDS | 50.0% | |
| GCTTGTGTTCTGCGTATGGT+TGG | + | chr5.3:73832778-73832797 | MS.gene017137:CDS | 50.0% | |
| TAGCCACTGTTGGTCATCCA+AGG | - | chr5.3:73832571-73832590 | None:intergenic | 50.0% | |
| CCAACTGAGCTAAGCTCACG+TGG | + | chr5.3:73832240-73832259 | MS.gene017137:intron | 55.0% | |
| CCACGTGAGCTTAGCTCAGT+TGG | - | chr5.3:73832243-73832262 | None:intergenic | 55.0% | |
| !! | AAGAAGCATTACTCCGGGCC+TGG | - | chr5.3:73832420-73832439 | None:intergenic | 55.0% |
| AAGGTCCGGGGTTCGAATCC+CGG | - | chr5.3:73832197-73832216 | None:intergenic | 60.0% | |
| GGTGGCCGGGATTCGAACCC+CGG | + | chr5.3:73832189-73832208 | MS.gene017137:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 73832005 | 73833139 | 73832005 | ID=MS.gene017137 |
| chr5.3 | mRNA | 73832005 | 73833139 | 73832005 | ID=MS.gene017137.t1;Parent=MS.gene017137 |
| chr5.3 | exon | 73832005 | 73832051 | 73832005 | ID=MS.gene017137.t1.exon1;Parent=MS.gene017137.t1 |
| chr5.3 | CDS | 73832005 | 73832051 | 73832005 | ID=cds.MS.gene017137.t1;Parent=MS.gene017137.t1 |
| chr5.3 | exon | 73832421 | 73832447 | 73832421 | ID=MS.gene017137.t1.exon2;Parent=MS.gene017137.t1 |
| chr5.3 | CDS | 73832421 | 73832447 | 73832421 | ID=cds.MS.gene017137.t1;Parent=MS.gene017137.t1 |
| chr5.3 | exon | 73832533 | 73832655 | 73832533 | ID=MS.gene017137.t1.exon3;Parent=MS.gene017137.t1 |
| chr5.3 | CDS | 73832533 | 73832655 | 73832533 | ID=cds.MS.gene017137.t1;Parent=MS.gene017137.t1 |
| chr5.3 | exon | 73832754 | 73833003 | 73832754 | ID=MS.gene017137.t1.exon4;Parent=MS.gene017137.t1 |
| chr5.3 | CDS | 73832754 | 73833003 | 73832754 | ID=cds.MS.gene017137.t1;Parent=MS.gene017137.t1 |
| chr5.3 | exon | 73833113 | 73833139 | 73833113 | ID=MS.gene017137.t1.exon5;Parent=MS.gene017137.t1 |
| chr5.3 | CDS | 73833113 | 73833139 | 73833113 | ID=cds.MS.gene017137.t1;Parent=MS.gene017137.t1 |
| Gene Sequence |
| Protein sequence |
