Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017150.t1 | XP_024638953.1 | 95.9 | 319 | 13 | 0 | 55 | 373 | 5 | 323 | 7.60E-175 | 623.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017150.t1 | Q4V3D9 | 57.1 | 345 | 105 | 2 | 72 | 373 | 30 | 374 | 2.1e-109 | 397.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017150.t1 | G7K3F5 | 95.9 | 319 | 13 | 0 | 55 | 373 | 5 | 323 | 5.5e-175 | 623.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene061292 | MS.gene017150 | PPI |
| MS.gene017150 | MS.gene35719 | PPI |
| MS.gene034366 | MS.gene017150 | PPI |
| MS.gene017150 | MS.gene61630 | PPI |
| MS.gene059296 | MS.gene017150 | PPI |
| MS.gene66625 | MS.gene017150 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017150.t1 | MTR_5g091980 | 95.925 | 319 | 13 | 0 | 55 | 373 | 5 | 323 | 0.0 | 627 |
| MS.gene017150.t1 | MTR_7g490350 | 69.505 | 364 | 64 | 3 | 57 | 373 | 6 | 369 | 8.07e-179 | 501 |
| MS.gene017150.t1 | MTR_7g090300 | 68.956 | 364 | 66 | 3 | 57 | 373 | 6 | 369 | 8.53e-177 | 496 |
| MS.gene017150.t1 | MTR_7g490330 | 74.455 | 321 | 78 | 2 | 56 | 373 | 6 | 325 | 4.35e-174 | 488 |
| MS.gene017150.t1 | MTR_2g087780 | 48.580 | 352 | 127 | 7 | 73 | 373 | 47 | 395 | 1.73e-103 | 311 |
| MS.gene017150.t1 | MTR_4g050190 | 46.724 | 351 | 134 | 7 | 73 | 373 | 44 | 391 | 8.12e-97 | 293 |
| MS.gene017150.t1 | MTR_7g090320 | 42.470 | 332 | 137 | 5 | 95 | 372 | 55 | 386 | 3.12e-85 | 264 |
| MS.gene017150.t1 | MTR_7g090310 | 41.210 | 347 | 150 | 6 | 80 | 372 | 39 | 385 | 4.09e-85 | 263 |
| MS.gene017150.t1 | MTR_2g099030 | 44.548 | 321 | 127 | 5 | 98 | 368 | 82 | 401 | 8.57e-84 | 261 |
| MS.gene017150.t1 | MTR_4g112670 | 39.466 | 337 | 172 | 4 | 57 | 371 | 8 | 334 | 1.49e-76 | 239 |
| MS.gene017150.t1 | MTR_2g058450 | 44.300 | 307 | 150 | 4 | 88 | 373 | 30 | 336 | 3.16e-72 | 229 |
| MS.gene017150.t1 | MTR_4g112630 | 41.176 | 289 | 157 | 5 | 97 | 372 | 37 | 325 | 3.11e-71 | 226 |
| MS.gene017150.t1 | MTR_4g112650 | 39.796 | 294 | 155 | 4 | 97 | 368 | 37 | 330 | 1.26e-67 | 217 |
| MS.gene017150.t1 | MTR_4g112620 | 36.552 | 290 | 140 | 4 | 97 | 372 | 41 | 300 | 2.49e-54 | 181 |
| MS.gene017150.t1 | MTR_2g058410 | 39.565 | 230 | 121 | 3 | 159 | 370 | 1 | 230 | 2.94e-42 | 147 |
| MS.gene017150.t1 | MTR_0003s0530 | 37.158 | 183 | 94 | 3 | 203 | 365 | 2 | 183 | 5.37e-34 | 125 |
| MS.gene017150.t1 | MTR_8g074270 | 35.371 | 229 | 127 | 5 | 98 | 326 | 78 | 285 | 4.61e-32 | 124 |
| MS.gene017150.t1 | MTR_8g074170 | 34.783 | 230 | 127 | 6 | 98 | 326 | 78 | 285 | 5.09e-31 | 121 |
| MS.gene017150.t1 | MTR_8g074210 | 33.478 | 230 | 132 | 4 | 97 | 326 | 77 | 285 | 5.20e-29 | 116 |
| MS.gene017150.t1 | MTR_4g112840 | 45.361 | 97 | 53 | 0 | 236 | 332 | 63 | 159 | 8.79e-24 | 97.1 |
| MS.gene017150.t1 | MTR_2g042370 | 29.210 | 291 | 156 | 8 | 123 | 373 | 77 | 357 | 2.67e-22 | 97.1 |
| MS.gene017150.t1 | MTR_2g042430 | 29.739 | 306 | 160 | 10 | 108 | 369 | 70 | 364 | 1.49e-21 | 95.1 |
| MS.gene017150.t1 | MTR_2g042420 | 28.522 | 291 | 157 | 8 | 123 | 373 | 70 | 349 | 3.27e-21 | 94.0 |
| MS.gene017150.t1 | MTR_1g075865 | 37.008 | 127 | 71 | 3 | 207 | 331 | 7 | 126 | 7.90e-19 | 82.4 |
| MS.gene017150.t1 | MTR_5g037520 | 81.481 | 54 | 10 | 0 | 1 | 54 | 299 | 352 | 2.02e-18 | 86.7 |
| MS.gene017150.t1 | MTR_8g074320 | 36.434 | 129 | 77 | 3 | 156 | 281 | 95 | 221 | 1.58e-15 | 75.5 |
| MS.gene017150.t1 | MTR_2g058430 | 40.206 | 97 | 56 | 1 | 150 | 244 | 52 | 148 | 4.71e-15 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017150.t1 | AT3G57030 | 56.936 | 346 | 106 | 2 | 71 | 373 | 29 | 374 | 1.65e-137 | 397 |
| MS.gene017150.t1 | AT5G22020 | 47.801 | 341 | 127 | 5 | 81 | 373 | 58 | 395 | 2.37e-98 | 298 |
| MS.gene017150.t1 | AT5G22020 | 47.801 | 341 | 127 | 5 | 81 | 373 | 58 | 395 | 2.37e-98 | 298 |
| MS.gene017150.t1 | AT5G22020 | 47.801 | 341 | 127 | 5 | 81 | 373 | 58 | 395 | 2.37e-98 | 298 |
| MS.gene017150.t1 | AT1G08470 | 46.286 | 350 | 134 | 7 | 74 | 373 | 44 | 389 | 6.46e-98 | 296 |
| MS.gene017150.t1 | AT3G57010 | 44.414 | 367 | 151 | 8 | 52 | 370 | 6 | 367 | 3.17e-89 | 273 |
| MS.gene017150.t1 | AT2G41290 | 43.902 | 369 | 151 | 7 | 60 | 373 | 9 | 376 | 1.52e-88 | 272 |
| MS.gene017150.t1 | AT2G41300 | 46.602 | 309 | 119 | 4 | 105 | 370 | 85 | 390 | 1.97e-87 | 270 |
| MS.gene017150.t1 | AT3G57020 | 44.510 | 337 | 139 | 6 | 78 | 370 | 34 | 366 | 5.77e-87 | 268 |
| MS.gene017150.t1 | AT3G57020 | 42.730 | 337 | 131 | 6 | 78 | 370 | 34 | 352 | 8.24e-80 | 249 |
| MS.gene017150.t1 | AT3G59530 | 47.925 | 265 | 125 | 4 | 71 | 323 | 53 | 316 | 7.11e-77 | 243 |
| MS.gene017150.t1 | AT3G59530 | 47.925 | 265 | 125 | 4 | 71 | 323 | 53 | 316 | 7.11e-77 | 243 |
| MS.gene017150.t1 | AT3G59530 | 47.925 | 265 | 125 | 4 | 71 | 323 | 53 | 316 | 7.11e-77 | 243 |
| MS.gene017150.t1 | AT1G74020 | 41.980 | 293 | 150 | 6 | 98 | 373 | 38 | 327 | 8.13e-67 | 214 |
| MS.gene017150.t1 | AT1G74010 | 40.273 | 293 | 154 | 7 | 98 | 373 | 37 | 325 | 3.97e-62 | 202 |
| MS.gene017150.t1 | AT1G74000 | 39.456 | 294 | 158 | 7 | 97 | 373 | 39 | 329 | 4.30e-61 | 199 |
| MS.gene017150.t1 | AT3G51440 | 32.812 | 256 | 149 | 7 | 99 | 350 | 70 | 306 | 1.55e-30 | 120 |
| MS.gene017150.t1 | AT3G51430 | 32.171 | 258 | 155 | 6 | 74 | 330 | 45 | 283 | 1.77e-30 | 120 |
| MS.gene017150.t1 | AT3G51430 | 30.797 | 276 | 170 | 7 | 74 | 347 | 45 | 301 | 2.30e-30 | 119 |
| MS.gene017150.t1 | AT3G51450 | 32.189 | 233 | 138 | 6 | 99 | 330 | 70 | 283 | 3.79e-29 | 116 |
| MS.gene017150.t1 | AT3G51420 | 31.416 | 226 | 135 | 6 | 99 | 323 | 70 | 276 | 1.30e-26 | 109 |
Find 93 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACTTCCTTTGAGAGATTT+TGG | 0.170650 | 5.3:+73976749 | MS.gene017150:CDS |
| ATTCTTCGACTTTGGCTTCA+TGG | 0.205561 | 5.3:+73992043 | MS.gene017150:CDS |
| TAGGTGAAACTTCGACTTTC+CGG | 0.217649 | 5.3:+73992020 | MS.gene017150:CDS |
| CTGTCCTTTGATCCAGAAAT+TGG | 0.265268 | 5.3:-73978409 | None:intergenic |
| CTTCTTAGCGGAGACAAAAC+TGG | 0.298940 | 5.3:+73991890 | MS.gene017150:CDS |
| CTTTCCGGATTCTTCGACTT+TGG | 0.322440 | 5.3:+73992035 | MS.gene017150:CDS |
| ATATTGACTTGGCCAGCATT+AGG | 0.334062 | 5.3:-73992067 | None:intergenic |
| TCCATTCGCACCAGAGTTAG+AGG | 0.369138 | 5.3:+73991284 | MS.gene017150:CDS |
| CAGGTTATCCGGACAATATA+AGG | 0.377879 | 5.3:+73992104 | MS.gene017150:CDS |
| TATTGACTTGGCCAGCATTA+GGG | 0.387491 | 5.3:-73992066 | None:intergenic |
| TACTGTTCTTCCAGGTTATC+CGG | 0.387839 | 5.3:+73992093 | MS.gene017150:CDS |
| GATGGTTTATTTCTTCTAGT+AGG | 0.394434 | 5.3:+73992001 | MS.gene017150:CDS |
| CCATCCGCCACGCCGGTGTA+TGG | 0.411524 | 5.3:-73978511 | None:intergenic |
| CTCCATGTCACCGGAGCCGT+TGG | 0.420609 | 5.3:+73978451 | MS.gene017150:CDS |
| GGTGTTTGTTTAAGCAAAGA+TGG | 0.423443 | 5.3:+73991983 | MS.gene017150:CDS |
| TGGCTTCATGGCCCTAATGC+TGG | 0.424712 | 5.3:+73992055 | MS.gene017150:CDS |
| TTGCTTAAACAAACACCATT+GGG | 0.427535 | 5.3:-73991977 | None:intergenic |
| GCTATAAAGTTGAGTGATGA+AGG | 0.432202 | 5.3:+73992169 | MS.gene017150:CDS |
| TTGCTGTCACTAGTTCCAAT+AGG | 0.439270 | 5.3:+73978578 | MS.gene017150:CDS |
| CTTATATTGTCCGGATAACC+TGG | 0.443834 | 5.3:-73992103 | None:intergenic |
| CTCTGATTACTGATATGATT+AGG | 0.445203 | 5.3:+73976594 | None:intergenic |
| TTCACTAATGTCCATGTCAT+TGG | 0.446239 | 5.3:-73991460 | None:intergenic |
| GGATATTTGTGGGAGGCCAC+TGG | 0.449445 | 5.3:+73991305 | MS.gene017150:CDS |
| CCAGAGTTAGAGGATATTTG+TGG | 0.459327 | 5.3:+73991294 | MS.gene017150:CDS |
| GAATTTCTCCTTATATTGTC+CGG | 0.460285 | 5.3:-73992112 | None:intergenic |
| ACTCTCCGGCCCAACGGCTC+CGG | 0.460954 | 5.3:-73978461 | None:intergenic |
| TTTGCTTAAACAAACACCAT+TGG | 0.461079 | 5.3:-73991978 | None:intergenic |
| GAAGTGAATGTCTTATTAAG+TGG | 0.467731 | 5.3:+73991944 | MS.gene017150:CDS |
| TGAAGAGCCGTGCGAGGAAG+TGG | 0.469737 | 5.3:+73976718 | MS.gene017150:CDS |
| TATATTGTTGATGCCTACCT+TGG | 0.481015 | 5.3:+73991360 | MS.gene017150:CDS |
| TCCTCTAACTCTGGTGCGAA+TGG | 0.487071 | 5.3:-73991285 | None:intergenic |
| GAAGCCAAAGTCGAAGAATC+CGG | 0.487945 | 5.3:-73992039 | None:intergenic |
| AAACTATAAATACCAACATA+AGG | 0.492238 | 5.3:-73992286 | None:intergenic |
| GCCCAACGGCTCCGGTGACA+TGG | 0.497514 | 5.3:-73978453 | None:intergenic |
| AGAAGGATAACATCTCTCAT+AGG | 0.503698 | 5.3:-73976695 | None:intergenic |
| TGGATCAAAGGACAGTCTCC+AGG | 0.505663 | 5.3:+73978417 | MS.gene017150:CDS |
| CCACAAATATCCTCTAACTC+TGG | 0.512177 | 5.3:-73991294 | None:intergenic |
| CTGCAGGTCCTACTACGTTA+AGG | 0.516853 | 5.3:-73991383 | None:intergenic |
| TGAAGAGCCGTGTGAGAAAG+TGG | 0.522120 | 5.3:+73976664 | MS.gene017150:CDS |
| ATGTCATTGGTGAAGCGAAA+AGG | 0.526768 | 5.3:-73991447 | None:intergenic |
| TCCATGTCACCGGAGCCGTT+GGG | 0.527613 | 5.3:+73978452 | MS.gene017150:CDS |
| TCTCACACGGCTCTTCAAGA+AGG | 0.532683 | 5.3:-73976658 | None:intergenic |
| TGTCACCGGAGCCGTTGGGC+CGG | 0.534102 | 5.3:+73978456 | MS.gene017150:CDS |
| CTTAACGTAGTAGGACCTGC+AGG | 0.540126 | 5.3:+73991384 | MS.gene017150:CDS |
| GATATTTGTGGGAGGCCACT+GGG | 0.541095 | 5.3:+73991306 | MS.gene017150:CDS |
| ACACCGGCGTGGCGGATGGA+CGG | 0.542691 | 5.3:+73978515 | MS.gene017150:CDS |
| CCATACACCGGCGTGGCGGA+TGG | 0.548384 | 5.3:+73978511 | MS.gene017150:CDS |
| CGCTTCACTGACATATCTCA+TGG | 0.548468 | 5.3:-73992221 | None:intergenic |
| AAGTGAATGTCTTATTAAGT+GGG | 0.550293 | 5.3:+73991945 | MS.gene017150:CDS |
| GGTTCCAATTTCTGGATCAA+AGG | 0.550697 | 5.3:+73978405 | MS.gene017150:CDS |
| TACCTTGGCCTTAACGTAGT+AGG | 0.551747 | 5.3:+73991375 | MS.gene017150:CDS |
| CAGAGTTAGAGGATATTTGT+GGG | 0.555272 | 5.3:+73991295 | MS.gene017150:CDS |
| AGGGGAAATATTAGAAATCG+TGG | 0.558229 | 5.3:+73992189 | MS.gene017150:CDS |
| CCTCGCACGGCTCTTCAAGA+AGG | 0.558443 | 5.3:-73976712 | None:intergenic |
| CATGGAGTAACCGATCAGCC+TGG | 0.564584 | 5.3:-73978435 | None:intergenic |
| TTCTTAGCGGAGACAAAACT+GGG | 0.567664 | 5.3:+73991891 | MS.gene017150:CDS |
| AGGACAGTCTCCAGGCTGAT+CGG | 0.569322 | 5.3:+73978425 | MS.gene017150:CDS |
| AGTGGGCTTAATTATCCCAA+TGG | 0.572203 | 5.3:+73991962 | MS.gene017150:CDS |
| AAGTATGAAGGGGAGGAACG+TGG | 0.583448 | 5.3:+73978544 | MS.gene017150:CDS |
| CAAGCAGAGTCTATCAGAGA+AGG | 0.584929 | 5.3:+73991511 | MS.gene017150:CDS |
| TATAAAGTTGAGTGATGAAG+GGG | 0.593424 | 5.3:+73992171 | MS.gene017150:CDS |
| GTCCTACTACGTTAAGGCCA+AGG | 0.595729 | 5.3:-73991377 | None:intergenic |
| AATCCGTCCATCCGCCACGC+CGG | 0.596699 | 5.3:-73978518 | None:intergenic |
| AATATAAGGAGAAATTCAGA+TGG | 0.600334 | 5.3:+73992118 | MS.gene017150:CDS |
| TTATCAAATCTTAGTCCCAG+TGG | 0.602844 | 5.3:-73991321 | None:intergenic |
| GACTTACCAAAATCTCTCAA+AGG | 0.605839 | 5.3:-73976755 | None:intergenic |
| GAAATATTAGAAATCGTGGA+AGG | 0.606501 | 5.3:+73992193 | MS.gene017150:CDS |
| ATGAAGGGGAGGAACGTGGT+TGG | 0.613290 | 5.3:+73978548 | MS.gene017150:CDS |
| CATTGGTGAAGCGAAAAGGT+TGG | 0.618180 | 5.3:-73991443 | None:intergenic |
| GATCGGTTACTCCATGTCAC+CGG | 0.620224 | 5.3:+73978442 | MS.gene017150:CDS |
| AGCTTCTGTTGCTAACTGTG+TGG | 0.621050 | 5.3:-73991415 | None:intergenic |
| TCGGTGTTAATGCCTTATGT+TGG | 0.621568 | 5.3:+73992274 | MS.gene017150:CDS |
| GTGCAAGAAAAGTTGCAGCA+TGG | 0.622116 | 5.3:-73978351 | None:intergenic |
| CTATAAAGTTGAGTGATGAA+GGG | 0.622219 | 5.3:+73992170 | MS.gene017150:CDS |
| AACAAGACTCTCCGGCCCAA+CGG | 0.631984 | 5.3:-73978467 | None:intergenic |
| TCTGCTACCACTTCCTCGCA+CGG | 0.638474 | 5.3:-73976725 | None:intergenic |
| CCTTCTTGAAGAGCCGTGCG+AGG | 0.639878 | 5.3:+73976712 | MS.gene017150:CDS |
| TTAACGTAGTAGGACCTGCA+GGG | 0.641562 | 5.3:+73991385 | MS.gene017150:CDS |
| CATGAGATATGTCAGTGAAG+CGG | 0.643807 | 5.3:+73992222 | MS.gene017150:CDS |
| CAGTTAGCAACAGAAGCTGA+AGG | 0.665226 | 5.3:+73991420 | MS.gene017150:CDS |
| ATATAAGGAGAAATTCAGAT+GGG | 0.667598 | 5.3:+73992119 | MS.gene017150:CDS |
| TCTCCGCTAAGAAGTAAAAG+CGG | 0.672637 | 5.3:-73991881 | None:intergenic |
| AATTTCAAGGTCGAACTGTG+TGG | 0.674077 | 5.3:+73991260 | MS.gene017150:intron |
| TACTACGTTAAGGCCAAGGT+AGG | 0.676893 | 5.3:-73991373 | None:intergenic |
| CACGGCGAAGGACCATACAC+CGG | 0.690062 | 5.3:+73978499 | MS.gene017150:CDS |
| TAACGTAGTAGGACCTGCAG+GGG | 0.691917 | 5.3:+73991386 | MS.gene017150:CDS |
| AGTTAGAGGATATTTGTGGG+AGG | 0.696868 | 5.3:+73991298 | MS.gene017150:CDS |
| GATTAGGTCTAAAGAGACAG+CGG | 0.705530 | 5.3:+73976610 | MS.gene017150:CDS |
| AGGACCATACACCGGCGTGG+CGG | 0.710184 | 5.3:+73978507 | MS.gene017150:CDS |
| AACGTAGTAGGACCTGCAGG+GGG | 0.714485 | 5.3:+73991387 | MS.gene017150:CDS |
| CGAAGGACCATACACCGGCG+TGG | 0.716462 | 5.3:+73978504 | MS.gene017150:CDS |
| ACTGCTACCACTTTCTCACA+CGG | 0.741910 | 5.3:-73976671 | None:intergenic |
| TTAGCGGAGACAAAACTGGG+AGG | 0.751407 | 5.3:+73991894 | MS.gene017150:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 73976608 | 73992312 | 73976608 | ID=MS.gene017150 |
| chr5.3 | mRNA | 73976608 | 73992312 | 73976608 | ID=MS.gene017150.t1;Parent=MS.gene017150 |
| chr5.3 | exon | 73976608 | 73976770 | 73976608 | ID=MS.gene017150.t1.exon1;Parent=MS.gene017150.t1 |
| chr5.3 | CDS | 73976608 | 73976770 | 73976608 | ID=cds.MS.gene017150.t1;Parent=MS.gene017150.t1 |
| chr5.3 | exon | 73978344 | 73978599 | 73978344 | ID=MS.gene017150.t1.exon2;Parent=MS.gene017150.t1 |
| chr5.3 | CDS | 73978344 | 73978599 | 73978344 | ID=cds.MS.gene017150.t1;Parent=MS.gene017150.t1 |
| chr5.3 | exon | 73991269 | 73991532 | 73991269 | ID=MS.gene017150.t1.exon3;Parent=MS.gene017150.t1 |
| chr5.3 | CDS | 73991269 | 73991532 | 73991269 | ID=cds.MS.gene017150.t1;Parent=MS.gene017150.t1 |
| chr5.3 | exon | 73991874 | 73992312 | 73991874 | ID=MS.gene017150.t1.exon4;Parent=MS.gene017150.t1 |
| chr5.3 | CDS | 73991874 | 73992312 | 73991874 | ID=cds.MS.gene017150.t1;Parent=MS.gene017150.t1 |
| Gene Sequence |
| Protein sequence |
