AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene034366

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034366.t1 MTR_2g087780 95.489 399 13 2 1 397 1 396 0.0 778
MS.gene034366.t1 MTR_4g050190 77.468 395 83 3 4 396 1 391 0.0 634
MS.gene034366.t1 MTR_7g090300 50.141 355 159 7 47 396 28 369 4.10e-112 333
MS.gene034366.t1 MTR_7g490350 50.000 356 158 8 47 396 28 369 6.00e-112 332
MS.gene034366.t1 MTR_2g099030 44.103 390 203 8 6 386 13 396 2.40e-102 309
MS.gene034366.t1 MTR_7g490330 47.953 342 123 6 60 396 34 325 3.53e-99 298
MS.gene034366.t1 MTR_5g091980 46.197 355 132 7 47 396 23 323 1.67e-94 286
MS.gene034366.t1 MTR_7g090310 42.560 336 180 8 69 395 54 385 2.06e-84 263
MS.gene034366.t1 MTR_7g090320 43.844 333 173 9 73 395 58 386 6.90e-83 259
MS.gene034366.t1 MTR_4g112670 37.353 340 171 10 63 394 29 334 7.66e-64 207
MS.gene034366.t1 MTR_4g112650 36.937 333 166 10 71 394 36 333 1.62e-58 194
MS.gene034366.t1 MTR_4g112630 36.036 333 160 8 71 394 36 324 1.23e-57 191
MS.gene034366.t1 MTR_2g058450 42.857 238 124 6 71 301 38 270 9.09e-54 181
MS.gene034366.t1 MTR_8g074170 34.084 311 169 7 13 321 27 303 1.63e-40 147
MS.gene034366.t1 MTR_8g074270 34.405 311 168 7 13 321 27 303 4.01e-40 146
MS.gene034366.t1 MTR_4g112620 31.532 333 146 9 71 394 40 299 8.07e-40 144
MS.gene034366.t1 MTR_8g074210 31.414 382 214 11 13 392 27 362 8.43e-40 145
MS.gene034366.t1 MTR_2g058410 41.317 167 94 3 139 301 1 167 6.64e-31 118
MS.gene034366.t1 MTR_2g042370 28.302 265 157 7 146 396 112 357 2.02e-27 112
MS.gene034366.t1 MTR_0003s0530 31.100 209 114 5 183 388 2 183 1.47e-25 103
MS.gene034366.t1 MTR_8g074320 41.085 129 72 2 136 261 95 222 1.65e-25 103
MS.gene034366.t1 MTR_2g042420 29.412 255 166 9 146 396 105 349 1.86e-25 106
MS.gene034366.t1 MTR_2g042430 30.400 250 162 8 146 392 124 364 5.43e-25 105
MS.gene034366.t1 MTR_1g075865 44.762 105 56 1 187 289 7 111 1.11e-23 95.9
MS.gene034366.t1 MTR_4g112840 37.500 96 58 1 208 301 56 151 5.32e-16 75.5
MS.gene034366.t1 MTR_2g058430 50.633 79 38 1 130 207 52 130 2.97e-15 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034366.t1 AT1G08470 66.067 389 125 3 10 396 6 389 0.0 542
MS.gene034366.t1 AT5G22020 64.380 379 128 3 20 396 22 395 0.0 512
MS.gene034366.t1 AT5G22020 64.380 379 128 3 20 396 22 395 0.0 512
MS.gene034366.t1 AT5G22020 64.380 379 128 3 20 396 22 395 0.0 512
MS.gene034366.t1 AT3G57030 50.000 344 157 5 58 396 41 374 4.75e-116 343
MS.gene034366.t1 AT3G59530 42.462 398 205 7 4 393 13 394 1.86e-101 306
MS.gene034366.t1 AT3G59530 42.462 398 205 7 4 393 13 394 1.86e-101 306
MS.gene034366.t1 AT3G59530 42.462 398 205 7 4 393 13 394 1.86e-101 306
MS.gene034366.t1 AT3G57010 46.224 331 155 8 71 393 52 367 9.65e-92 281
MS.gene034366.t1 AT3G57020 45.921 331 156 10 71 393 51 366 2.84e-88 272
MS.gene034366.t1 AT2G41300 44.410 322 159 7 78 393 83 390 1.01e-86 269
MS.gene034366.t1 AT3G57020 43.807 331 149 8 71 393 51 352 1.31e-82 257
MS.gene034366.t1 AT2G41290 41.520 342 166 9 73 396 51 376 1.25e-78 247
MS.gene034366.t1 AT1G74010 37.202 336 160 13 69 396 33 325 8.05e-49 168
MS.gene034366.t1 AT1G74000 34.535 333 166 11 73 396 40 329 6.71e-47 163
MS.gene034366.t1 AT1G74020 33.934 333 170 12 72 396 37 327 3.25e-44 156
MS.gene034366.t1 AT3G51420 28.994 338 205 8 57 392 53 357 1.36e-33 129
MS.gene034366.t1 AT3G51440 32.959 267 153 7 56 319 52 295 9.09e-31 121
MS.gene034366.t1 AT3G51430 29.773 309 184 10 13 319 18 295 1.34e-29 118
MS.gene034366.t1 AT3G51430 31.301 246 145 6 57 300 53 276 7.12e-29 116
MS.gene034366.t1 AT3G51450 30.189 265 161 6 57 319 53 295 1.67e-28 115

Find 108 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATTCAGTTGATACTTTC+TGG 0.149122 2.4:+13374481 MS.gene034366:CDS
TACATAGCAGATGCATATTT+CGG 0.221241 2.4:+13374178 MS.gene034366:CDS
GACATCTGCGGGAGGCCTTT+GGG 0.272968 2.4:+13374124 MS.gene034366:CDS
GGACATCTGCGGGAGGCCTT+TGG 0.299116 2.4:+13374123 MS.gene034366:CDS
ACATAGCAGATGCATATTTC+GGG 0.314837 2.4:+13374179 MS.gene034366:CDS
CTAAGCAAGTACTGGCTAAA+AGG 0.321104 2.4:+13374897 MS.gene034366:intron
ATACTTTCTGGTGATAATAA+TGG 0.334662 2.4:+13374493 MS.gene034366:CDS
TTGGATAGGGCTTTGCTTGA+AGG 0.344074 2.4:-13373374 None:intergenic
TTGCTTAAGGAAATCCCATT+TGG 0.360210 2.4:-13374580 None:intergenic
CGGTAAACTTCTGCAAATAT+TGG 0.362581 2.4:+13375163 MS.gene034366:CDS
TGGGATAGGTAGCTTGAGTA+TGG 0.373674 2.4:-13375072 None:intergenic
ATTCAATACCTGCTACATAT+TGG 0.377715 2.4:+13375101 MS.gene034366:CDS
CAACGAAAATGGTGACCTTT+GGG 0.392894 2.4:+13374983 MS.gene034366:CDS
TTCGTTGACTCTCACATTGT+CGG 0.397838 2.4:-13374967 None:intergenic
CACAAGAACAGTGGTTTCCT+TGG 0.403080 2.4:-13374542 None:intergenic
CTAAGATTTGACAAGAAAAC+TGG 0.404603 2.4:+13374148 MS.gene034366:CDS
AGAAATGTTCAATTTCCAAA+TGG 0.409686 2.4:+13374565 MS.gene034366:CDS
TCAACGAAAATGGTGACCTT+TGG 0.415508 2.4:+13374982 MS.gene034366:CDS
ATCATGGTCTGAAGTTCCTC+TGG 0.416576 2.4:+13373435 MS.gene034366:CDS
GGGTGGCTATCCATTGTAGA+AGG 0.420650 2.4:+13375003 MS.gene034366:CDS
TGCTCTCCGGTCCCTGCACT+TGG 0.422805 2.4:-13373507 None:intergenic
TGGATAGGGCTTTGCTTGAA+GGG 0.424691 2.4:-13373373 None:intergenic
AGTGGTGTTGCAGATGCCTT+TGG 0.424846 2.4:-13374082 None:intergenic
GAAGGAACCATCCTTGCTTA+AGG 0.426528 2.4:-13374593 None:intergenic
TTGCATATACCTCGCCCAAC+AGG 0.446476 2.4:+13373617 MS.gene034366:CDS
GAAATGTTCAATTTCCAAAT+GGG 0.451526 2.4:+13374566 MS.gene034366:CDS
CCATCAGCAACACCAGTGTA+AGG 0.454210 2.4:-13373556 None:intergenic
TTTGTAGGCTAAGCAAGTAC+TGG 0.457715 2.4:+13374889 MS.gene034366:intron
AATGTGAGAGTCAACGAAAA+TGG 0.457761 2.4:+13374972 MS.gene034366:CDS
CTGATGAAGGTGGGGCCTCA+AGG 0.459786 2.4:+13374202 MS.gene034366:CDS
GCAGGTATTGAATCCTTGTT+GGG 0.464036 2.4:-13375091 None:intergenic
ATTTGCAGAAGTTTACCGTC+AGG 0.467604 2.4:-13375158 None:intergenic
GAAGAGAAGCGTGACCTGTT+GGG 0.474973 2.4:-13373631 None:intergenic
ACAAAATTAACTTGCCATCA+TGG 0.475567 2.4:+13373419 MS.gene034366:CDS
CCTCAAAATCAGGGAAGCCT+TGG 0.484602 2.4:-13373393 None:intergenic
TATTGAATCCTTGTTGGGAT+AGG 0.492693 2.4:-13375086 None:intergenic
TGTACATATACCTTCTACAA+TGG 0.493786 2.4:-13375013 None:intergenic
TGCATATTTCGGGCTGATGA+AGG 0.499203 2.4:+13374189 MS.gene034366:CDS
AGCATTGCCTTCGATTATCA+TGG 0.499411 2.4:+13373526 MS.gene034366:CDS
TAATCGAAGGCAATGCTCTC+CGG 0.499769 2.4:-13373520 None:intergenic
AAGAACAGTGGTTTCCTTGG+TGG 0.500187 2.4:-13374539 None:intergenic
ACGTCATTAGTAAATCTAAA+CGG 0.500664 2.4:-13374265 None:intergenic
AGTGAGTGAAGTGGAGGAGA+AGG 0.503333 2.4:+13375223 MS.gene034366:CDS
AGTGAAGTGGAGGAGAAGGA+TGG 0.504056 2.4:+13375227 MS.gene034366:CDS
CGCAACATCGCTTGCAACTG+AGG 0.505541 2.4:+13374231 MS.gene034366:CDS
GGGATTTCCTTAAGCAAGGA+TGG 0.509307 2.4:+13374586 MS.gene034366:CDS
AGCAGGTATTGAATCCTTGT+TGG 0.510712 2.4:-13375092 None:intergenic
AAATCAGGGAAGCCTTGGAT+AGG 0.512244 2.4:-13373388 None:intergenic
CCTTACACTGGTGTTGCTGA+TGG 0.513319 2.4:+13373556 MS.gene034366:CDS
GCCTTCGATTATCATGGCCG+TGG 0.516919 2.4:+13373532 MS.gene034366:CDS
GAAATCTTAGCAATCCTACC+TGG 0.521950 2.4:+13374939 MS.gene034366:CDS
TGGCTAAAAGGAGATAAAGC+TGG 0.528508 2.4:+13374909 MS.gene034366:CDS
CATGGCCGTGGCCCTTACAC+TGG 0.529185 2.4:+13373544 MS.gene034366:CDS
CTCTTGTTTGCTCTCTACTG+TGG 0.530078 2.4:+13373343 MS.gene034366:CDS
GGATAGGGCTTTGCTTGAAG+GGG 0.530593 2.4:-13373372 None:intergenic
AAATGGGATTTCCTTAAGCA+AGG 0.531261 2.4:+13374582 MS.gene034366:CDS
GCAAATATTGGAGGACAATG+AGG 0.532562 2.4:+13375175 MS.gene034366:CDS
TTGTCAAATCTTAGTCCCAA+AGG 0.536364 2.4:-13374139 None:intergenic
AAATGATAAACTGCAATGAA+AGG 0.537931 2.4:-13375278 None:intergenic
CATGTTTGTGAACCAAGTGC+AGG 0.540963 2.4:+13373495 MS.gene034366:CDS
TAAACTTCTGCAAATATTGG+AGG 0.544725 2.4:+13375166 MS.gene034366:CDS
CTCACATTGTCGGCAAAACC+AGG 0.545473 2.4:-13374957 None:intergenic
TTTCAGAAGGAGTAATTGGA+AGG 0.549054 2.4:+13374623 MS.gene034366:CDS
TATTTCGGGCTGATGAAGGT+GGG 0.549565 2.4:+13374193 MS.gene034366:CDS
AATCAGGGAAGCCTTGGATA+GGG 0.552957 2.4:-13373387 None:intergenic
ACACTGGTGTTGCTGATGGA+AGG 0.553412 2.4:+13373560 MS.gene034366:CDS
AAGGATGGTAAACTCTGGAT+AGG 0.555622 2.4:+13375242 MS.gene034366:CDS
TCCTTCTGAAAATACAAAGA+AGG 0.555909 2.4:-13374611 None:intergenic
TGTGAACCAAGTGCAGGGAC+CGG 0.556419 2.4:+13373501 MS.gene034366:CDS
CAATACCTGCTACATATTGG+AGG 0.561063 2.4:+13375104 MS.gene034366:CDS
TTCTGGTGATAATAATGGAA+GGG 0.568249 2.4:+13374498 MS.gene034366:CDS
GAAGCGTGACCTGTTGGGCG+AGG 0.570009 2.4:-13373626 None:intergenic
TTTGCAGAAGTTTACCGTCA+GGG 0.572710 2.4:-13375157 None:intergenic
GACGTAGACGTTGATGCAGA+AGG 0.584580 2.4:+13374283 MS.gene034366:CDS
GAGTGAAGTGAGTGAAGTGG+AGG 0.588505 2.4:+13375217 MS.gene034366:CDS
TTTCTGGTGATAATAATGGA+AGG 0.588715 2.4:+13374497 MS.gene034366:CDS
AGTGAGTGAAGTGAGTGAAG+TGG 0.595807 2.4:+13375214 MS.gene034366:CDS
CAAATATTGGAGGACAATGA+GGG 0.596205 2.4:+13375176 MS.gene034366:CDS
GAAATACAATTCTGCCACCA+AGG 0.601190 2.4:+13374525 MS.gene034366:CDS
CAACACCAGTGTAAGGGCCA+CGG 0.604427 2.4:-13373549 None:intergenic
ATTTCGGGCTGATGAAGGTG+GGG 0.604505 2.4:+13374194 MS.gene034366:CDS
AGGAGAAGGATGGTAAACTC+TGG 0.608335 2.4:+13375237 MS.gene034366:CDS
CATCAGCAACACCAGTGTAA+GGG 0.608492 2.4:-13373555 None:intergenic
GTCGTTAAGTATAGCCCTGA+CGG 0.613243 2.4:+13375143 MS.gene034366:CDS
GCCACGGCCATGATAATCGA+AGG 0.615291 2.4:-13373533 None:intergenic
TCAGTGTCAACATAACTAAG+TGG 0.615561 2.4:-13374100 None:intergenic
ACGTAGACGTTGATGCAGAA+GGG 0.617394 2.4:+13374284 MS.gene034366:CDS
TTGTCTTTGTCATGATCCAG+AGG 0.617900 2.4:-13373451 None:intergenic
TTGACACTGAGGACATCTGC+GGG 0.621256 2.4:+13374113 MS.gene034366:CDS
ATATTTCGGGCTGATGAAGG+TGG 0.623437 2.4:+13374192 MS.gene034366:CDS
ATGAAGGTGGGGCCTCAAGG+TGG 0.624685 2.4:+13374205 MS.gene034366:CDS
AACATTTCTCACAAGAACAG+TGG 0.629432 2.4:-13374551 None:intergenic
TCGCTTGCAACTGAGGCAGA+AGG 0.629466 2.4:+13374238 MS.gene034366:CDS
GTCAGGACTATGCAATCCAA+AGG 0.629677 2.4:+13374066 MS.gene034366:CDS
TTCAAGCAAAGCCCTATCCA+AGG 0.630560 2.4:+13373376 MS.gene034366:CDS
GCAGCACTAAATATCAGAGA+AGG 0.631433 2.4:+13374326 MS.gene034366:CDS
ATGTTTGTGAACCAAGTGCA+GGG 0.633422 2.4:+13373496 MS.gene034366:CDS
AGAGGAACTTCAGACCATGA+TGG 0.637623 2.4:-13373433 None:intergenic
TACAATGGATAGCCACCCAA+AGG 0.641529 2.4:-13374998 None:intergenic
CGAAAATGGTGACCTTTGGG+TGG 0.646638 2.4:+13374986 MS.gene034366:CDS
GTTGACACTGAGGACATCTG+CGG 0.649702 2.4:+13374112 MS.gene034366:CDS
CATTGTCGGCAAAACCAGGT+AGG 0.653789 2.4:-13374953 None:intergenic
TCAAGCTACCTATCCCAACA+AGG 0.673835 2.4:+13375078 MS.gene034366:CDS
TCATACATGACGCTAACTCG+AGG 0.676086 2.4:+13373304 MS.gene034366:CDS
GTCAGGGCTATACTTAACGA+CGG 0.676277 2.4:-13375141 None:intergenic
ACACTGAGGACATCTGCGGG+AGG 0.694284 2.4:+13374116 MS.gene034366:CDS
ACTTAGTTATGTTGACACTG+AGG 0.721000 2.4:+13374102 MS.gene034366:CDS
GATGTTGCGAAACCACCTTG+AGG 0.764503 2.4:-13374217 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAATAGACATGAATT+AGG - chr2.4:13374863-13374882 None:intergenic 15.0%
!!! TAAAATGTTGATAATTTCAA+AGG - chr2.4:13374385-13374404 None:intergenic 15.0%
!! ATAAGATGATGAAATATGAT+TGG + chr2.4:13374779-13374798 MS.gene034366:intron 20.0%
!! TATAGTTTCTAACTTAATGT+TGG + chr2.4:13373827-13373846 MS.gene034366:intron 20.0%
!!! TTTAAGTTGGAAAAGTTATA+AGG - chr2.4:13373660-13373679 None:intergenic 20.0%
! AAATGATAAACTGCAATGAA+AGG - chr2.4:13375281-13375300 None:intergenic 25.0%
! ACGTCATTAGTAAATCTAAA+CGG - chr2.4:13374268-13374287 None:intergenic 25.0%
! AGAAATAGCAAAACAATGTT+TGG + chr2.4:13374411-13374430 MS.gene034366:intron 25.0%
! AGAAATGTTCAATTTCCAAA+TGG + chr2.4:13374565-13374584 MS.gene034366:CDS 25.0%
! ATCGTTATTAGACGAAAAAA+GGG - chr2.4:13374669-13374688 None:intergenic 25.0%
! GAAATGTTCAATTTCCAAAT+GGG + chr2.4:13374566-13374585 MS.gene034366:CDS 25.0%
! TAAGATGAATGGATAATTTG+TGG + chr2.4:13373985-13374004 MS.gene034366:intron 25.0%
! TATCGTTATTAGACGAAAAA+AGG - chr2.4:13374670-13374689 None:intergenic 25.0%
!! AAGACTTTTCTTAAGATGAA+TGG + chr2.4:13373974-13373993 MS.gene034366:intron 25.0%
!! ATACTTTCTGGTGATAATAA+TGG + chr2.4:13374493-13374512 MS.gene034366:CDS 25.0%
ATAGTGCAAAACCTATTAGT+AGG + chr2.4:13373781-13373800 MS.gene034366:intron 30.0%
ATTCAATACCTGCTACATAT+TGG + chr2.4:13375101-13375120 MS.gene034366:CDS 30.0%
CTAAGATTTGACAAGAAAAC+TGG + chr2.4:13374148-13374167 MS.gene034366:CDS 30.0%
CTTGTACTAAGCATTTAAGT+TGG - chr2.4:13373673-13373692 None:intergenic 30.0%
TAAACTTCTGCAAATATTGG+AGG + chr2.4:13375166-13375185 MS.gene034366:CDS 30.0%
TACATAGCAGATGCATATTT+CGG + chr2.4:13374178-13374197 MS.gene034366:CDS 30.0%
TAGTGCAAAACCTATTAGTA+GGG + chr2.4:13373782-13373801 MS.gene034366:intron 30.0%
TGTACATATACCTTCTACAA+TGG - chr2.4:13375016-13375035 None:intergenic 30.0%
TTCTAAACATTGTGTCAGAA+TGG - chr2.4:13374739-13374758 None:intergenic 30.0%
TTTAATAGTATGAGCGTATG+AGG - chr2.4:13373878-13373897 None:intergenic 30.0%
! AGTATGGCTTTTCTAATCTT+TGG - chr2.4:13375059-13375078 None:intergenic 30.0%
! GTATGGCTTTTCTAATCTTT+GGG - chr2.4:13375058-13375077 None:intergenic 30.0%
! GTATTTTCAGAAGGAGTAAT+TGG + chr2.4:13374619-13374638 MS.gene034366:CDS 30.0%
! TCCTTCTGAAAATACAAAGA+AGG - chr2.4:13374614-13374633 None:intergenic 30.0%
! TCCTTCTTTGTATTTTCAGA+AGG + chr2.4:13374610-13374629 MS.gene034366:CDS 30.0%
! TTCATTCAGTTGATACTTTC+TGG + chr2.4:13374481-13374500 MS.gene034366:CDS 30.0%
! TTCTGGTGATAATAATGGAA+GGG + chr2.4:13374498-13374517 MS.gene034366:CDS 30.0%
! TTTCTGGTGATAATAATGGA+AGG + chr2.4:13374497-13374516 MS.gene034366:CDS 30.0%
!! ACAAAATTAACTTGCCATCA+TGG + chr2.4:13373419-13373438 MS.gene034366:CDS 30.0%
!!! TCTGTAACTTTTTCACAGAT+CGG - chr2.4:13373918-13373937 None:intergenic 30.0%
AAATGGGATTTCCTTAAGCA+AGG + chr2.4:13374582-13374601 MS.gene034366:CDS 35.0%
AACATTTCTCACAAGAACAG+TGG - chr2.4:13374554-13374573 None:intergenic 35.0%
AATGTGAGAGTCAACGAAAA+TGG + chr2.4:13374972-13374991 MS.gene034366:CDS 35.0%
ACATAGCAGATGCATATTTC+GGG + chr2.4:13374179-13374198 MS.gene034366:CDS 35.0%
ACTTAGTTATGTTGACACTG+AGG + chr2.4:13374102-13374121 MS.gene034366:CDS 35.0%
AGTGCAAAACCTATTAGTAG+GGG + chr2.4:13373783-13373802 MS.gene034366:intron 35.0%
ATAGCAAAACAATGTTTGGC+TGG + chr2.4:13374415-13374434 MS.gene034366:intron 35.0%
CAAATATTGGAGGACAATGA+GGG + chr2.4:13375176-13375195 MS.gene034366:CDS 35.0%
CACATATATCCCCTACTAAT+AGG - chr2.4:13373795-13373814 None:intergenic 35.0%
CGGTAAACTTCTGCAAATAT+TGG + chr2.4:13375163-13375182 MS.gene034366:CDS 35.0%
CTATTAGTAGGGGATATATG+TGG + chr2.4:13373793-13373812 MS.gene034366:intron 35.0%
GTCAGAATGGCAAAATATGT+TGG - chr2.4:13374726-13374745 None:intergenic 35.0%
TATTGAATCCTTGTTGGGAT+AGG - chr2.4:13375089-13375108 None:intergenic 35.0%
TCAGTGTCAACATAACTAAG+TGG - chr2.4:13374103-13374122 None:intergenic 35.0%
TTGCTTAAGGAAATCCCATT+TGG - chr2.4:13374583-13374602 None:intergenic 35.0%
TTGTCAAATCTTAGTCCCAA+AGG - chr2.4:13374142-13374161 None:intergenic 35.0%
TTTCAGAAGGAGTAATTGGA+AGG + chr2.4:13374623-13374642 MS.gene034366:CDS 35.0%
! ATTTTGTGCACCTCAAAATC+AGG - chr2.4:13373406-13373425 None:intergenic 35.0%
! TTTTGTGCACCTCAAAATCA+GGG - chr2.4:13373405-13373424 None:intergenic 35.0%
!! CTGATGGAAGGATTTTGTTT+TGG + chr2.4:13373572-13373591 MS.gene034366:CDS 35.0%
!!! GGAAGGATTTTGTTTTGGAA+TGG + chr2.4:13373577-13373596 MS.gene034366:CDS 35.0%
AAGGATGGTAAACTCTGGAT+AGG + chr2.4:13375242-13375261 MS.gene034366:CDS 40.0%
AGCATTGCCTTCGATTATCA+TGG + chr2.4:13373526-13373545 MS.gene034366:CDS 40.0%
ATGTTTGTGAACCAAGTGCA+GGG + chr2.4:13373496-13373515 MS.gene034366:CDS 40.0%
ATTCTGCTACATTGCATCTG+AGG + chr2.4:13373951-13373970 MS.gene034366:intron 40.0%
ATTTGCAGAAGTTTACCGTC+AGG - chr2.4:13375161-13375180 None:intergenic 40.0%
CAACGAAAATGGTGACCTTT+GGG + chr2.4:13374983-13375002 MS.gene034366:CDS 40.0%
CAATACCTGCTACATATTGG+AGG + chr2.4:13375104-13375123 MS.gene034366:CDS 40.0%
CTGACCTGTGTTCAGAAATA+AGG - chr2.4:13374051-13374070 None:intergenic 40.0%
GAAATACAATTCTGCCACCA+AGG + chr2.4:13374525-13374544 MS.gene034366:CDS 40.0%
GAAATCTTAGCAATCCTACC+TGG + chr2.4:13374939-13374958 MS.gene034366:CDS 40.0%
GCAAATATTGGAGGACAATG+AGG + chr2.4:13375175-13375194 MS.gene034366:CDS 40.0%
GCAGCACTAAATATCAGAGA+AGG + chr2.4:13374326-13374345 MS.gene034366:CDS 40.0%
GTACAATGACATCAGAAGTC+AGG - chr2.4:13374451-13374470 None:intergenic 40.0%
TCAACGAAAATGGTGACCTT+TGG + chr2.4:13374982-13375001 MS.gene034366:CDS 40.0%
TCTGATGTCATTGTACAAGC+AGG + chr2.4:13374455-13374474 MS.gene034366:intron 40.0%
TGGCTAAAAGGAGATAAAGC+TGG + chr2.4:13374909-13374928 MS.gene034366:CDS 40.0%
TTGTCTTTGTCATGATCCAG+AGG - chr2.4:13373454-13373473 None:intergenic 40.0%
TTTGCAGAAGTTTACCGTCA+GGG - chr2.4:13375160-13375179 None:intergenic 40.0%
TTTGTAGGCTAAGCAAGTAC+TGG + chr2.4:13374889-13374908 MS.gene034366:intron 40.0%
! TTTTGCCTCCAATATGTAGC+AGG - chr2.4:13375112-13375131 None:intergenic 40.0%
!! AGCAGGTATTGAATCCTTGT+TGG - chr2.4:13375095-13375114 None:intergenic 40.0%
!! CTAAGCAAGTACTGGCTAAA+AGG + chr2.4:13374897-13374916 MS.gene034366:intron 40.0%
!! CTTATTTCTGAACACAGGTC+AGG + chr2.4:13374049-13374068 MS.gene034366:intron 40.0%
!! GCAGGTATTGAATCCTTGTT+GGG - chr2.4:13375094-13375113 None:intergenic 40.0%
!! TTCGTTGACTCTCACATTGT+CGG - chr2.4:13374970-13374989 None:intergenic 40.0%
!!! CAATGTTTGGCTGGTTTTGT+TGG + chr2.4:13374424-13374443 MS.gene034366:intron 40.0%
!!! TGTTTTGGAATGGACTGTCT+TGG + chr2.4:13373587-13373606 MS.gene034366:CDS 40.0%
AAATCAGGGAAGCCTTGGAT+AGG - chr2.4:13373391-13373410 None:intergenic 45.0%
AAGAACAGTGGTTTCCTTGG+TGG - chr2.4:13374542-13374561 None:intergenic 45.0%
AATCAGGGAAGCCTTGGATA+GGG - chr2.4:13373390-13373409 None:intergenic 45.0%
ACGTAGACGTTGATGCAGAA+GGG + chr2.4:13374284-13374303 MS.gene034366:CDS 45.0%
AGAGGAACTTCAGACCATGA+TGG - chr2.4:13373436-13373455 None:intergenic 45.0%
AGGAGAAGGATGGTAAACTC+TGG + chr2.4:13375237-13375256 MS.gene034366:CDS 45.0%
AGTGAGTGAAGTGAGTGAAG+TGG + chr2.4:13375214-13375233 MS.gene034366:CDS 45.0%
ATATTTCGGGCTGATGAAGG+TGG + chr2.4:13374192-13374211 MS.gene034366:CDS 45.0%
CACAAGAACAGTGGTTTCCT+TGG - chr2.4:13374545-13374564 None:intergenic 45.0%
CATCAGCAACACCAGTGTAA+GGG - chr2.4:13373558-13373577 None:intergenic 45.0%
CATGTTTGTGAACCAAGTGC+AGG + chr2.4:13373495-13373514 MS.gene034366:CDS 45.0%
GAAGGAACCATCCTTGCTTA+AGG - chr2.4:13374596-13374615 None:intergenic 45.0%
GGGATTTCCTTAAGCAAGGA+TGG + chr2.4:13374586-13374605 MS.gene034366:CDS 45.0%
GTCAGGACTATGCAATCCAA+AGG + chr2.4:13374066-13374085 MS.gene034366:CDS 45.0%
GTCAGGGCTATACTTAACGA+CGG - chr2.4:13375144-13375163 None:intergenic 45.0%
GTCGTTAAGTATAGCCCTGA+CGG + chr2.4:13375143-13375162 MS.gene034366:CDS 45.0%
TAATCGAAGGCAATGCTCTC+CGG - chr2.4:13373523-13373542 None:intergenic 45.0%
TACAATGGATAGCCACCCAA+AGG - chr2.4:13375001-13375020 None:intergenic 45.0%
TATTTCGGGCTGATGAAGGT+GGG + chr2.4:13374193-13374212 MS.gene034366:CDS 45.0%
TCAAGCTACCTATCCCAACA+AGG + chr2.4:13375078-13375097 MS.gene034366:CDS 45.0%
TCATACATGACGCTAACTCG+AGG + chr2.4:13373304-13373323 MS.gene034366:CDS 45.0%
TGCATATTTCGGGCTGATGA+AGG + chr2.4:13374189-13374208 MS.gene034366:CDS 45.0%
TGGATAGGGCTTTGCTTGAA+GGG - chr2.4:13373376-13373395 None:intergenic 45.0%
TGGGATAGGTAGCTTGAGTA+TGG - chr2.4:13375075-13375094 None:intergenic 45.0%
TTCAAGCAAAGCCCTATCCA+AGG + chr2.4:13373376-13373395 MS.gene034366:CDS 45.0%
TTGGATAGGGCTTTGCTTGA+AGG - chr2.4:13373377-13373396 None:intergenic 45.0%
! ATCATGGTCTGAAGTTCCTC+TGG + chr2.4:13373435-13373454 MS.gene034366:CDS 45.0%
! CTCTTGTTTGCTCTCTACTG+TGG + chr2.4:13373343-13373362 MS.gene034366:CDS 45.0%
!! CGCACCTTATTTCTGAACAC+AGG + chr2.4:13374044-13374063 MS.gene034366:intron 45.0%
!!! ATTTTTTTTTTTTTTTTTGT+AGG + chr2.4:13374874-13374893 MS.gene034366:intron 5.0%
AGTGAAGTGGAGGAGAAGGA+TGG + chr2.4:13375227-13375246 MS.gene034366:CDS 50.0%
AGTGAGTGAAGTGGAGGAGA+AGG + chr2.4:13375223-13375242 MS.gene034366:CDS 50.0%
ATTTCGGGCTGATGAAGGTG+GGG + chr2.4:13374194-13374213 MS.gene034366:CDS 50.0%
CATTGTCGGCAAAACCAGGT+AGG - chr2.4:13374956-13374975 None:intergenic 50.0%
CCATCAGCAACACCAGTGTA+AGG - chr2.4:13373559-13373578 None:intergenic 50.0%
CCTCAAAATCAGGGAAGCCT+TGG - chr2.4:13373396-13373415 None:intergenic 50.0%
CGAAAATGGTGACCTTTGGG+TGG + chr2.4:13374986-13375005 MS.gene034366:CDS 50.0%
CTCACATTGTCGGCAAAACC+AGG - chr2.4:13374960-13374979 None:intergenic 50.0%
GACGTAGACGTTGATGCAGA+AGG + chr2.4:13374283-13374302 MS.gene034366:CDS 50.0%
GAGTGAAGTGAGTGAAGTGG+AGG + chr2.4:13375217-13375236 MS.gene034366:CDS 50.0%
GATGTTGCGAAACCACCTTG+AGG - chr2.4:13374220-13374239 None:intergenic 50.0%
GGATAGGGCTTTGCTTGAAG+GGG - chr2.4:13373375-13373394 None:intergenic 50.0%
GGGTGGCTATCCATTGTAGA+AGG + chr2.4:13375003-13375022 MS.gene034366:CDS 50.0%
GTTGACACTGAGGACATCTG+CGG + chr2.4:13374112-13374131 MS.gene034366:CDS 50.0%
TTGACACTGAGGACATCTGC+GGG + chr2.4:13374113-13374132 MS.gene034366:CDS 50.0%
TTGCATATACCTCGCCCAAC+AGG + chr2.4:13373617-13373636 MS.gene034366:CDS 50.0%
! AGAAGAGAAGCGTGACCTGT+TGG - chr2.4:13373635-13373654 None:intergenic 50.0%
! CCAAGGCTTCCCTGATTTTG+AGG + chr2.4:13373393-13373412 MS.gene034366:CDS 50.0%
! GAAGAGAAGCGTGACCTGTT+GGG - chr2.4:13373634-13373653 None:intergenic 50.0%
!! ACACTGGTGTTGCTGATGGA+AGG + chr2.4:13373560-13373579 MS.gene034366:CDS 50.0%
!! AGTGGTGTTGCAGATGCCTT+TGG - chr2.4:13374085-13374104 None:intergenic 50.0%
!! CCTTACACTGGTGTTGCTGA+TGG + chr2.4:13373556-13373575 MS.gene034366:CDS 50.0%
CAACACCAGTGTAAGGGCCA+CGG - chr2.4:13373552-13373571 None:intergenic 55.0%
CGCAACATCGCTTGCAACTG+AGG + chr2.4:13374231-13374250 MS.gene034366:CDS 55.0%
GCCACGGCCATGATAATCGA+AGG - chr2.4:13373536-13373555 None:intergenic 55.0%
GCCTTCGATTATCATGGCCG+TGG + chr2.4:13373532-13373551 MS.gene034366:CDS 55.0%
TCGCTTGCAACTGAGGCAGA+AGG + chr2.4:13374238-13374257 MS.gene034366:CDS 55.0%
TGTGAACCAAGTGCAGGGAC+CGG + chr2.4:13373501-13373520 MS.gene034366:CDS 55.0%
ACACTGAGGACATCTGCGGG+AGG + chr2.4:13374116-13374135 MS.gene034366:CDS 60.0%
ATGAAGGTGGGGCCTCAAGG+TGG + chr2.4:13374205-13374224 MS.gene034366:CDS 60.0%
CTGATGAAGGTGGGGCCTCA+AGG + chr2.4:13374202-13374221 MS.gene034366:CDS 60.0%
GACATCTGCGGGAGGCCTTT+GGG + chr2.4:13374124-13374143 MS.gene034366:CDS 60.0%
CATGGCCGTGGCCCTTACAC+TGG + chr2.4:13373544-13373563 MS.gene034366:CDS 65.0%
GAAGCGTGACCTGTTGGGCG+AGG - chr2.4:13373629-13373648 None:intergenic 65.0%
GGACATCTGCGGGAGGCCTT+TGG + chr2.4:13374123-13374142 MS.gene034366:CDS 65.0%
TGCTCTCCGGTCCCTGCACT+TGG - chr2.4:13373510-13373529 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 13373301 13375307 13373301 ID=MS.gene034366
chr2.4 mRNA 13373301 13375307 13373301 ID=MS.gene034366.t1;Parent=MS.gene034366
chr2.4 exon 13373301 13373638 13373301 ID=MS.gene034366.t1.exon1;Parent=MS.gene034366.t1
chr2.4 CDS 13373301 13373638 13373301 ID=cds.MS.gene034366.t1;Parent=MS.gene034366.t1
chr2.4 exon 13374066 13374347 13374066 ID=MS.gene034366.t1.exon2;Parent=MS.gene034366.t1
chr2.4 CDS 13374066 13374347 13374066 ID=cds.MS.gene034366.t1;Parent=MS.gene034366.t1
chr2.4 exon 13374477 13374644 13374477 ID=MS.gene034366.t1.exon3;Parent=MS.gene034366.t1
chr2.4 CDS 13374477 13374644 13374477 ID=cds.MS.gene034366.t1;Parent=MS.gene034366.t1
chr2.4 exon 13374902 13375307 13374902 ID=MS.gene034366.t1.exon4;Parent=MS.gene034366.t1
chr2.4 CDS 13374902 13375307 13374902 ID=cds.MS.gene034366.t1;Parent=MS.gene034366.t1
Gene Sequence

>MS.gene034366

ATGTCATACATGACGCTAACTCGAGGACTCGCTATTGTAATTCTCTTGTTTGCTCTCTACTGTGGATTAGACCCCTTCAAGCAAAGCCCTATCCAAGGCTTCCCTGATTTTGAGGTGCACAAAATTAACTTGCCATCATGGTCTGAAGTTCCTCTGGATCATGACAAAGACAACTTGTTGAAGAAATCTGAGCTCATGTTTGTGAACCAAGTGCAGGGACCGGAGAGCATTGCCTTCGATTATCATGGCCGTGGCCCTTACACTGGTGTTGCTGATGGAAGGATTTTGTTTTGGAATGGACTGTCTTGGATTGATTTTGCATATACCTCGCCCAACAGGTCACGCTTCTCTTCTTTCCTTATAACTTTTCCAACTTAAATGCTTAGTACAAGATAATTTTGTGATTTTGTTATCTTTCTGCATTACTAGAATACTGTCAATAGTTATCAAATTCATAAGCTATGCTATAGTCATATTCTAATAGTGCAAAACCTATTAGTAGGGGATATATGTGGCTAAAAGCTACTATAGTTTCTAACTTAATGTTGGAAAACTGCTTCATGCAAACTTTTTACCTCATACGCTCATACTATTAAAGATCTCATGCTCTTGCACCGATCTGTGAAAAAGTTACAGAAAATATGTGTGAAATTCTGCTACATTGCATCTGAGGAAGACTTTTCTTAAGATGAATGGATAATTTGTGGCAATGTAACTTTGCGATATCATGTGATAATATTTCTCGCACCTTATTTCTGAACACAGGTCAGGACTATGCAATCCAAAGGCATCTGCAACACCACTTAGTTATGTTGACACTGAGGACATCTGCGGGAGGCCTTTGGGACTAAGATTTGACAAGAAAACTGGTGATTTATACATAGCAGATGCATATTTCGGGCTGATGAAGGTGGGGCCTCAAGGTGGTTTCGCAACATCGCTTGCAACTGAGGCAGAAGGAGTGCCGTTTAGATTTACTAATGACGTAGACGTTGATGCAGAAGGGAACGTTTACTTCACAGATAGCAGCACTAAATATCAGAGAAGGTTGTTAGTAATATTTTTTCTTGTTAACACTTCACCTTTGAAATTATCAACATTTTAAATATGAGAAATAGCAAAACAATGTTTGGCTGGTTTTGTTGGTCCTGACTTCTGATGTCATTGTACAAGCAGGAATTTCATTCAGTTGATACTTTCTGGTGATAATAATGGAAGGGTTTTGAAATACAATTCTGCCACCAAGGAAACCACTGTTCTTGTGAGAAATGTTCAATTTCCAAATGGGATTTCCTTAAGCAAGGATGGTTCCTTCTTTGTATTTTCAGAAGGAGTAATTGGAAGGTGTGTGAATAATCAACATAACCCTTTTTTCGTCTAATAACGATACACTAATGATGCAAACAAGTTTTACATAGACGTCCAACATATTTTGCCATTCTGACACAATGTTTAGAAAGTAATATTGTGATTATGCAATAAGATGATGAAATATGATTGGACATCTATGTAAAATTTATTTGCATCATAGCATAAAGTTATTGAACTTTTATTTTCTTCCTAATTCATGTCTATTTTTTTTTTTTTTTTTGTAGGCTAAGCAAGTACTGGCTAAAAGGAGATAAAGCTGGAACTTTAGAAATCTTAGCAATCCTACCTGGTTTTGCCGACAATGTGAGAGTCAACGAAAATGGTGACCTTTGGGTGGCTATCCATTGTAGAAGGTATATGTACAGTTACATAAATGCAATCTACCCAAAGATTAGAAAAGCCATACTCAAGCTACCTATCCCAACAAGGATTCAATACCTGCTACATATTGGAGGCAAAATGCATGCAGCCGTCGTTAAGTATAGCCCTGACGGTAAACTTCTGCAAATATTGGAGGACAATGAGGGAAAAGTTGTCAAAGCAGTGAGTGAAGTGAGTGAAGTGGAGGAGAAGGATGGTAAACTCTGGATAGGAAGTGTTTTGATGCCTTTCATTGCAGTTTATCATTTGACATGA
Protein sequence

>MS.gene034366.t1

MSYMTLTRGLAIVILLFALYCGLDPFKQSPIQGFPDFEVHKINLPSWSEVPLDHDKDNLLKKSELMFVNQVQGPESIAFDYHGRGPYTGVADGRILFWNGLSWIDFAYTSPNRSGLCNPKASATPLSYVDTEDICGRPLGLRFDKKTGDLYIADAYFGLMKVGPQGGFATSLATEAEGVPFRFTNDVDVDAEGNVYFTDSSTKYQRRNFIQLILSGDNNGRVLKYNSATKETTVLVRNVQFPNGISLSKDGSFFVFSEGVIGSKYWLKGDKAGTLEILAILPGFADNVRVNENGDLWVAIHCRRYMYSYINAIYPKIRKAILKLPIPTRIQYLLHIGGKMHAAVVKYSPDGKLLQILEDNEGKVVKAVSEVSEVEEKDGKLWIGSVLMPFIAVYHLT