AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35719

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35719.t1 MTR_2g087780 97.222 396 11 0 1 396 1 396 0.0 798
MS.gene35719.t1 MTR_4g050190 78.571 392 83 1 4 395 1 391 0.0 650
MS.gene35719.t1 MTR_7g090300 51.136 352 159 5 47 395 28 369 3.24e-118 348
MS.gene35719.t1 MTR_7g490350 50.852 352 160 5 47 395 28 369 4.36e-117 345
MS.gene35719.t1 MTR_2g099030 45.220 387 202 6 6 385 13 396 3.89e-109 327
MS.gene35719.t1 MTR_7g490330 48.968 339 123 4 60 395 34 325 2.54e-105 314
MS.gene35719.t1 MTR_5g091980 47.443 352 131 6 47 395 23 323 8.78e-101 302
MS.gene35719.t1 MTR_7g090310 42.943 333 182 6 69 394 54 385 1.83e-88 273
MS.gene35719.t1 MTR_7g090320 44.242 330 175 7 73 394 58 386 3.64e-88 272
MS.gene35719.t1 MTR_4g112670 37.313 335 177 7 63 393 29 334 5.59e-69 221
MS.gene35719.t1 MTR_4g112650 37.576 330 167 8 71 393 36 333 3.65e-64 208
MS.gene35719.t1 MTR_4g112630 36.667 330 161 6 71 393 36 324 2.56e-63 206
MS.gene35719.t1 MTR_2g058450 36.250 320 168 7 71 385 38 326 6.13e-58 192
MS.gene35719.t1 MTR_4g112620 32.110 327 151 6 71 393 40 299 2.39e-45 158
MS.gene35719.t1 MTR_8g074170 31.316 380 214 9 13 390 27 361 5.81e-43 154
MS.gene35719.t1 MTR_8g074210 31.926 379 215 10 13 391 27 362 9.19e-43 154
MS.gene35719.t1 MTR_8g074270 31.662 379 214 9 13 390 27 361 1.42e-42 153
MS.gene35719.t1 MTR_2g058410 34.375 256 140 4 139 392 1 230 3.56e-34 127
MS.gene35719.t1 MTR_0003s0530 32.039 206 115 3 183 387 2 183 3.28e-30 115
MS.gene35719.t1 MTR_2g042370 28.626 262 159 5 146 395 112 357 3.31e-30 119
MS.gene35719.t1 MTR_2g042420 29.762 252 168 7 146 395 105 349 3.09e-28 114
MS.gene35719.t1 MTR_2g042430 30.769 247 164 6 146 391 124 364 1.07e-27 112
MS.gene35719.t1 MTR_8g074320 41.085 129 72 2 136 261 95 222 1.46e-25 103
MS.gene35719.t1 MTR_1g075865 44.762 105 58 0 187 291 7 111 1.89e-25 100
MS.gene35719.t1 MTR_4g112840 37.500 96 60 0 208 303 56 151 6.63e-18 80.9
MS.gene35719.t1 MTR_2g058430 50.633 79 38 1 130 207 52 130 2.53e-15 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35719.t1 AT1G08470 67.358 386 124 1 10 395 6 389 0.0 559
MS.gene35719.t1 AT5G22020 65.426 376 128 1 20 395 22 395 0.0 526
MS.gene35719.t1 AT5G22020 65.426 376 128 1 20 395 22 395 0.0 526
MS.gene35719.t1 AT5G22020 65.426 376 128 1 20 395 22 395 0.0 526
MS.gene35719.t1 AT3G57030 50.733 341 158 3 58 395 41 374 8.24e-122 358
MS.gene35719.t1 AT3G59530 43.797 395 203 6 4 392 13 394 4.44e-107 321
MS.gene35719.t1 AT3G59530 43.797 395 203 6 4 392 13 394 4.44e-107 321
MS.gene35719.t1 AT3G59530 43.797 395 203 6 4 392 13 394 4.44e-107 321
MS.gene35719.t1 AT3G57010 46.201 329 157 7 71 392 52 367 1.56e-93 285
MS.gene35719.t1 AT3G57020 45.289 329 160 7 71 392 51 366 1.35e-90 278
MS.gene35719.t1 AT2G41300 44.828 319 161 5 78 392 83 390 2.09e-90 278
MS.gene35719.t1 AT3G57020 44.073 329 150 7 71 392 51 352 3.17e-85 263
MS.gene35719.t1 AT2G41290 41.983 343 162 10 73 395 51 376 3.05e-79 249
MS.gene35719.t1 AT1G74010 37.838 333 161 12 69 395 33 325 3.30e-52 177
MS.gene35719.t1 AT1G74000 34.650 329 170 9 73 395 40 329 4.42e-50 172
MS.gene35719.t1 AT1G74020 34.545 330 171 11 72 395 37 327 1.06e-47 166
MS.gene35719.t1 AT3G51420 29.254 335 207 7 57 391 53 357 2.66e-36 136
MS.gene35719.t1 AT3G51440 33.333 267 154 6 56 321 52 295 4.31e-34 130
MS.gene35719.t1 AT3G51430 30.097 309 185 9 13 321 18 295 1.69e-32 126
MS.gene35719.t1 AT3G51430 30.690 290 170 9 13 302 18 276 8.40e-32 124
MS.gene35719.t1 AT3G51450 30.566 265 162 5 57 321 53 295 9.42e-32 124

Find 109 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATTCAGTTGATACTTTC+TGG 0.149122 2.1:-14086629 MS.gene35719:CDS
TACATAGCAGATGCATATTT+CGG 0.221241 2.1:-14086932 MS.gene35719:CDS
GACATCTGCGGGAGGCCTTT+GGG 0.272968 2.1:-14086986 MS.gene35719:CDS
GGACATCTGCGGGAGGCCTT+TGG 0.299116 2.1:-14086987 MS.gene35719:CDS
ACATAGCAGATGCATATTTC+GGG 0.314837 2.1:-14086931 MS.gene35719:CDS
CTAAGCAAGTACTGGCTAAA+AGG 0.321104 2.1:-14086302 MS.gene35719:CDS
ATACTTTCTGGTGATAATAA+TGG 0.334662 2.1:-14086617 MS.gene35719:CDS
TTGGATAGGGCTTTGCTTGA+AGG 0.344074 2.1:+14087722 None:intergenic
TTGCTTAAGGAAATCCCATT+TGG 0.360210 2.1:+14086530 None:intergenic
CGGTAAACTTCTGCAAATAT+TGG 0.362581 2.1:-14086036 MS.gene35719:CDS
TGGGATAGGTAGCTTGAGTA+TGG 0.373674 2.1:+14086127 None:intergenic
ATTCAATACCTGCTACATAT+TGG 0.377715 2.1:-14086098 MS.gene35719:CDS
CAACGAAAATGGTGACCTTT+GGG 0.392894 2.1:-14086216 MS.gene35719:CDS
TTCGTTGACTCTCACATTGT+CGG 0.397838 2.1:+14086232 None:intergenic
CACAAGAACAGTGGTTTCCT+TGG 0.403080 2.1:+14086568 None:intergenic
CTAAGATTTGACAAGAAAAC+TGG 0.404603 2.1:-14086962 MS.gene35719:CDS
AGAAATGTTCAATTTCCAAA+TGG 0.409686 2.1:-14086545 MS.gene35719:CDS
TCAACGAAAATGGTGACCTT+TGG 0.415508 2.1:-14086217 MS.gene35719:CDS
ATCATGGTCTGAAGTTCCTC+TGG 0.416576 2.1:-14087661 MS.gene35719:CDS
GGGTGGCTATCCATTGTAGA+AGG 0.420650 2.1:-14086196 MS.gene35719:CDS
TGCTCTCCGGTCCCTGCACT+TGG 0.422805 2.1:+14087589 None:intergenic
TGGATAGGGCTTTGCTTGAA+GGG 0.424691 2.1:+14087723 None:intergenic
AGTGGTGTTGCAGATGCCTT+TGG 0.424846 2.1:+14087028 None:intergenic
GAAGGAACCATCCTTGCTTA+AGG 0.426528 2.1:+14086517 None:intergenic
AGTGAGTGAAGTGGAGGAGA+AGG 0.431067 2.1:-14085985 MS.gene35719:CDS
CCATCAGCAACACCGGTGTA+AGG 0.434665 2.1:+14087540 None:intergenic
AGACAACTTGTTGAAGAAAT+CGG 0.446631 2.1:-14087628 MS.gene35719:CDS
GAAATGTTCAATTTCCAAAT+GGG 0.451526 2.1:-14086544 MS.gene35719:CDS
AATGTGAGAGTCAACGAAAA+TGG 0.457761 2.1:-14086227 MS.gene35719:CDS
TTGCATACACCTCGCCCAAC+AGG 0.458425 2.1:-14087479 MS.gene35719:intron
CTGATGAAGGTGGGGCCTCA+AGG 0.459786 2.1:-14086908 MS.gene35719:CDS
GCAGGTATTGAATCCTTGTT+GGG 0.464036 2.1:+14086108 None:intergenic
TCTGTAGGCTAAGCAAGTAC+TGG 0.465199 2.1:-14086310 MS.gene35719:intron
ATTTGCAGAAGTTTACCGTC+AGG 0.467604 2.1:+14086041 None:intergenic
GAAGAGAAGCGTGACCTGTT+GGG 0.474973 2.1:+14087465 None:intergenic
ACAAAATTAACTTGCCATCA+TGG 0.475567 2.1:-14087677 MS.gene35719:CDS
CCTCAAAATCAGGGAAGCCT+TGG 0.484602 2.1:+14087703 None:intergenic
TATTGAATCCTTGTTGGGAT+AGG 0.492693 2.1:+14086113 None:intergenic
TGTACATATACCTTCTACAA+TGG 0.493786 2.1:+14086186 None:intergenic
TGCATATTTCGGGCTGATGA+AGG 0.499203 2.1:-14086921 MS.gene35719:CDS
AAGAACAGTGGTTTCCTTGG+TGG 0.500187 2.1:+14086571 None:intergenic
ACGTCATTAGTAAATCTAAA+CGG 0.500664 2.1:+14086845 None:intergenic
GAATCGAAGGCAATGCTCTC+CGG 0.502502 2.1:+14087576 None:intergenic
AGTGAAGTGGAGGAGAAGGA+TGG 0.504056 2.1:-14085981 MS.gene35719:CDS
CGCAACATCGCTTGCAACTG+AGG 0.505541 2.1:-14086879 MS.gene35719:CDS
GGGATTTCCTTAAGCAAGGA+TGG 0.509307 2.1:-14086524 MS.gene35719:CDS
AGCAGGTATTGAATCCTTGT+TGG 0.510712 2.1:+14086107 None:intergenic
AAATCAGGGAAGCCTTGGAT+AGG 0.512244 2.1:+14087708 None:intergenic
GAAATCTTAGCAATCCTACC+TGG 0.521950 2.1:-14086260 MS.gene35719:CDS
GCCTTCGATTCTCATGGTCG+TGG 0.522772 2.1:-14087564 MS.gene35719:CDS
TGGCTAAAAGGAGATAAAGC+TGG 0.528508 2.1:-14086290 MS.gene35719:CDS
CTCTTGTTTGCTCTCTACTG+TGG 0.530078 2.1:-14087753 MS.gene35719:CDS
GGATAGGGCTTTGCTTGAAG+GGG 0.530593 2.1:+14087724 None:intergenic
AAATGGGATTTCCTTAAGCA+AGG 0.531261 2.1:-14086528 MS.gene35719:CDS
TAAAGCAGTGAGTGAAGTGG+AGG 0.531558 2.1:-14085991 MS.gene35719:CDS
GCAAATATTGGAGGACAATG+AGG 0.532562 2.1:-14086024 MS.gene35719:CDS
TTGTCAAATCTTAGTCCCAA+AGG 0.536364 2.1:+14086971 None:intergenic
CATGTTTGTGAACCAAGTGC+AGG 0.540963 2.1:-14087601 MS.gene35719:CDS
AAATGATAAACCGCAATGAA+AGG 0.541109 2.1:+14085930 None:intergenic
TAAACTTCTGCAAATATTGG+AGG 0.544725 2.1:-14086033 MS.gene35719:CDS
CTCACATTGTCGGCAAAACC+AGG 0.545473 2.1:+14086242 None:intergenic
AGCATTGCCTTCGATTCTCA+TGG 0.549052 2.1:-14087570 MS.gene35719:CDS
TTTCAGAAGGAGTAATTGGA+AGG 0.549054 2.1:-14086487 MS.gene35719:intron
ACACCGGTGTTGCTGATGGA+AGG 0.549231 2.1:-14087536 MS.gene35719:CDS
TATTTCGGGCTGATGAAGGT+GGG 0.549565 2.1:-14086917 MS.gene35719:CDS
TGTTAAAGCAGTGAGTGAAG+TGG 0.551000 2.1:-14085994 MS.gene35719:CDS
AATCAGGGAAGCCTTGGATA+GGG 0.552957 2.1:+14087709 None:intergenic
AAGGATGGTAAACTCTGGAT+AGG 0.555622 2.1:-14085966 MS.gene35719:CDS
TCCTTCTGAAAATACAAAGA+AGG 0.555909 2.1:+14086499 None:intergenic
TGTGAACCAAGTGCAGGGAC+CGG 0.556419 2.1:-14087595 MS.gene35719:CDS
CATGGTCGTGGCCCTTACAC+CGG 0.556626 2.1:-14087552 MS.gene35719:CDS
CAATACCTGCTACATATTGG+AGG 0.561063 2.1:-14086095 MS.gene35719:CDS
GAAGCGTGACCTGTTGGGCG+AGG 0.565272 2.1:+14087470 None:intergenic
TTCTGGTGATAATAATGGAA+GGG 0.568249 2.1:-14086612 MS.gene35719:CDS
TTTGCAGAAGTTTACCGTCA+GGG 0.572710 2.1:+14086042 None:intergenic
CCTTACACCGGTGTTGCTGA+TGG 0.584330 2.1:-14087540 MS.gene35719:CDS
GACGTAGACGTTGATGCAGA+AGG 0.584580 2.1:-14086827 MS.gene35719:CDS
CATCAGCAACACCGGTGTAA+GGG 0.586543 2.1:+14087541 None:intergenic
TTTCTGGTGATAATAATGGA+AGG 0.588715 2.1:-14086613 MS.gene35719:CDS
GCCACGACCATGAGAATCGA+AGG 0.595727 2.1:+14087563 None:intergenic
CAAATATTGGAGGACAATGA+GGG 0.596205 2.1:-14086023 MS.gene35719:CDS
AATCCTTCCATCAGCAACAC+CGG 0.596968 2.1:+14087533 None:intergenic
GAAATACAATTCTGCCACCA+AGG 0.601190 2.1:-14086585 MS.gene35719:CDS
ATTTCGGGCTGATGAAGGTG+GGG 0.604505 2.1:-14086916 MS.gene35719:CDS
AGGAGAAGGATGGTAAACTC+TGG 0.608335 2.1:-14085971 MS.gene35719:CDS
GTCGTTAAGTATAGCCCTGA+CGG 0.613243 2.1:-14086056 MS.gene35719:CDS
TCAGTGTCAACATAACTAAG+TGG 0.615561 2.1:+14087010 None:intergenic
ACGTAGACGTTGATGCAGAA+GGG 0.617394 2.1:-14086826 MS.gene35719:CDS
TTGTCTTTGTCATGATCCAG+AGG 0.617900 2.1:+14087645 None:intergenic
TTGACACTGAGGACATCTGC+GGG 0.621256 2.1:-14086997 MS.gene35719:CDS
ATATTTCGGGCTGATGAAGG+TGG 0.623437 2.1:-14086918 MS.gene35719:CDS
ATGAAGGTGGGGCCTCAAGG+TGG 0.624685 2.1:-14086905 MS.gene35719:CDS
AACATTTCTCACAAGAACAG+TGG 0.629432 2.1:+14086559 None:intergenic
TCGCTTGCAACTGAGGCAGA+AGG 0.629466 2.1:-14086872 MS.gene35719:CDS
GTCAGAACTATGCAATCCAA+AGG 0.629677 2.1:-14087044 MS.gene35719:CDS
TTCAAGCAAAGCCCTATCCA+AGG 0.630560 2.1:-14087720 MS.gene35719:CDS
GCAGCACTAAATATCAGAGA+AGG 0.631433 2.1:-14086784 MS.gene35719:intron
ATGTTTGTGAACCAAGTGCA+GGG 0.633422 2.1:-14087600 MS.gene35719:CDS
AGAGGAACTTCAGACCATGA+TGG 0.637623 2.1:+14087663 None:intergenic
TACAATGGATAGCCACCCAA+AGG 0.641529 2.1:+14086201 None:intergenic
CGAAAATGGTGACCTTTGGG+TGG 0.646638 2.1:-14086213 MS.gene35719:CDS
GTTGACACTGAGGACATCTG+CGG 0.649702 2.1:-14086998 MS.gene35719:CDS
CATTGTCGGCAAAACCAGGT+AGG 0.653789 2.1:+14086246 None:intergenic
TCAAGCTACCTATCCCAACA+AGG 0.673835 2.1:-14086121 MS.gene35719:CDS
TCATACATGACGCTAACTCG+AGG 0.676086 2.1:-14087792 MS.gene35719:CDS
GTCAGGGCTATACTTAACGA+CGG 0.676277 2.1:+14086058 None:intergenic
ACACTGAGGACATCTGCGGG+AGG 0.694284 2.1:-14086994 MS.gene35719:CDS
ACTTAGTTATGTTGACACTG+AGG 0.721000 2.1:-14087008 MS.gene35719:CDS
GATGTTGCGAAACCACCTTG+AGG 0.764503 2.1:+14086893 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATTTCTAACTTAATGT+TGG - chr2.1:14086435-14086454 MS.gene35719:intron 15.0%
!!! TAAAATGTTGATAATTTCAA+AGG + chr2.1:14086993-14087012 None:intergenic 15.0%
!! TCAATAGTTATCAAATTCAT+AGG - chr2.1:14086351-14086370 MS.gene35719:intron 20.0%
!!! TTTAAGTTGGAAAAGTTATA+AGG + chr2.1:14086282-14086301 None:intergenic 20.0%
! ACGTCATTAGTAAATCTAAA+CGG + chr2.1:14086876-14086895 None:intergenic 25.0%
! AGAAATAGCAAAACAATGTT+TGG - chr2.1:14087019-14087038 MS.gene35719:CDS 25.0%
! AGAAATGTTCAATTTCCAAA+TGG - chr2.1:14087173-14087192 MS.gene35719:intron 25.0%
! ATCGTTATTAGACGAAAAAA+GGG + chr2.1:14087277-14087296 None:intergenic 25.0%
! GAAATGTTCAATTTCCAAAT+GGG - chr2.1:14087174-14087193 MS.gene35719:intron 25.0%
! TAAGATGAATGGATAATTTG+TGG - chr2.1:14086593-14086612 MS.gene35719:CDS 25.0%
! TATCGTTATTAGACGAAAAA+AGG + chr2.1:14087278-14087297 None:intergenic 25.0%
!! AAGACTTTTCTTAAGATGAA+TGG - chr2.1:14086582-14086601 MS.gene35719:CDS 25.0%
!! ATACTTTCTGGTGATAATAA+TGG - chr2.1:14087101-14087120 MS.gene35719:intron 25.0%
AAATGATAAACCGCAATGAA+AGG + chr2.1:14087791-14087810 None:intergenic 30.0%
ATTCAATACCTGCTACATAT+TGG - chr2.1:14087620-14087639 MS.gene35719:CDS 30.0%
CTAAACATATTGTGTCAGAA+TGG + chr2.1:14087347-14087366 None:intergenic 30.0%
CTAAGATTTGACAAGAAAAC+TGG - chr2.1:14086756-14086775 MS.gene35719:intron 30.0%
CTTGTACTAAGCATTTAAGT+TGG + chr2.1:14086295-14086314 None:intergenic 30.0%
TAAACTTCTGCAAATATTGG+AGG - chr2.1:14087685-14087704 MS.gene35719:CDS 30.0%
TACATAGCAGATGCATATTT+CGG - chr2.1:14086786-14086805 MS.gene35719:CDS 30.0%
TGTACATATACCTTCTACAA+TGG + chr2.1:14087535-14087554 None:intergenic 30.0%
! AGTATGGCTTTTCTAATCTT+TGG + chr2.1:14087578-14087597 None:intergenic 30.0%
! GTATGGCTTTTCTAATCTTT+GGG + chr2.1:14087577-14087596 None:intergenic 30.0%
! GTATTTTCAGAAGGAGTAAT+TGG - chr2.1:14087227-14087246 MS.gene35719:intron 30.0%
! TCCTTCTGAAAATACAAAGA+AGG + chr2.1:14087222-14087241 None:intergenic 30.0%
! TCCTTCTTTGTATTTTCAGA+AGG - chr2.1:14087218-14087237 MS.gene35719:intron 30.0%
! TTCATTCAGTTGATACTTTC+TGG - chr2.1:14087089-14087108 MS.gene35719:intron 30.0%
! TTCTGGTGATAATAATGGAA+GGG - chr2.1:14087106-14087125 MS.gene35719:intron 30.0%
! TTTCTGGTGATAATAATGGA+AGG - chr2.1:14087105-14087124 MS.gene35719:intron 30.0%
!! ACAAAATTAACTTGCCATCA+TGG - chr2.1:14086041-14086060 MS.gene35719:CDS 30.0%
!! AGACAACTTGTTGAAGAAAT+CGG - chr2.1:14086090-14086109 MS.gene35719:CDS 30.0%
!! CTGATGGAAGGATTTTATTT+TGG - chr2.1:14086194-14086213 MS.gene35719:CDS 30.0%
!!! GGAAGGATTTTATTTTGGAA+CGG - chr2.1:14086199-14086218 MS.gene35719:CDS 30.0%
!!! TCTGTAACTTTTTCACAGAT+CGG + chr2.1:14086526-14086545 None:intergenic 30.0%
AAATGGGATTTCCTTAAGCA+AGG - chr2.1:14087190-14087209 MS.gene35719:intron 35.0%
AACATTTCTCACAAGAACAG+TGG + chr2.1:14087162-14087181 None:intergenic 35.0%
AATAGTGCAAGCCTATTAGT+AGG - chr2.1:14086389-14086408 MS.gene35719:intron 35.0%
AATGTGAGAGTCAACGAAAA+TGG - chr2.1:14087491-14087510 MS.gene35719:CDS 35.0%
ACATAGCAGATGCATATTTC+GGG - chr2.1:14086787-14086806 MS.gene35719:CDS 35.0%
ACTTAGTTATGTTGACACTG+AGG - chr2.1:14086710-14086729 MS.gene35719:intron 35.0%
ATAGCAAAACAATGTTTGGC+TGG - chr2.1:14087023-14087042 MS.gene35719:CDS 35.0%
ATAGTGCAAGCCTATTAGTA+GGG - chr2.1:14086390-14086409 MS.gene35719:intron 35.0%
CAAATATTGGAGGACAATGA+GGG - chr2.1:14087695-14087714 MS.gene35719:CDS 35.0%
CACATATATCCCCTACTAAT+AGG + chr2.1:14086403-14086422 None:intergenic 35.0%
CGGTAAACTTCTGCAAATAT+TGG - chr2.1:14087682-14087701 MS.gene35719:CDS 35.0%
CTATTAGTAGGGGATATATG+TGG - chr2.1:14086401-14086420 MS.gene35719:intron 35.0%
GTCAGAATGGCAAAATATGT+TGG + chr2.1:14087334-14087353 None:intergenic 35.0%
TATTGAATCCTTGTTGGGAT+AGG + chr2.1:14087608-14087627 None:intergenic 35.0%
TCAGTGTCAACATAACTAAG+TGG + chr2.1:14086711-14086730 None:intergenic 35.0%
TGCTTAGCCTACAGAAAAAA+CGG + chr2.1:14087403-14087422 None:intergenic 35.0%
TTGCTTAAGGAAATCCCATT+TGG + chr2.1:14087191-14087210 None:intergenic 35.0%
TTGTCAAATCTTAGTCCCAA+AGG + chr2.1:14086750-14086769 None:intergenic 35.0%
TTTCAGAAGGAGTAATTGGA+AGG - chr2.1:14087231-14087250 MS.gene35719:intron 35.0%
! ATTTTGTGCACCTCAAAATC+AGG + chr2.1:14086028-14086047 None:intergenic 35.0%
! TTTTGTGCACCTCAAAATCA+GGG + chr2.1:14086027-14086046 None:intergenic 35.0%
!!! ATCAAGTCCGTTTTTTCTGT+AGG - chr2.1:14087393-14087412 MS.gene35719:intron 35.0%
!!! TGTTTTGATGCCTTTCATTG+CGG - chr2.1:14087778-14087797 MS.gene35719:CDS 35.0%
AAGGATGGTAAACTCTGGAT+AGG - chr2.1:14087752-14087771 MS.gene35719:CDS 40.0%
ATGTTTGTGAACCAAGTGCA+GGG - chr2.1:14086118-14086137 MS.gene35719:CDS 40.0%
ATTCTGCTACATTGCATCTG+AGG - chr2.1:14086559-14086578 MS.gene35719:CDS 40.0%
ATTTGCAGAAGTTTACCGTC+AGG + chr2.1:14087680-14087699 None:intergenic 40.0%
CAACGAAAATGGTGACCTTT+GGG - chr2.1:14087502-14087521 MS.gene35719:CDS 40.0%
CAATACCTGCTACATATTGG+AGG - chr2.1:14087623-14087642 MS.gene35719:CDS 40.0%
CTGACCTGTGTTCAGAAATA+AGG + chr2.1:14086659-14086678 None:intergenic 40.0%
GAAATACAATTCTGCCACCA+AGG - chr2.1:14087133-14087152 MS.gene35719:intron 40.0%
GAAATCTTAGCAATCCTACC+TGG - chr2.1:14087458-14087477 MS.gene35719:intron 40.0%
GCAAATATTGGAGGACAATG+AGG - chr2.1:14087694-14087713 MS.gene35719:CDS 40.0%
GCAGCACTAAATATCAGAGA+AGG - chr2.1:14086934-14086953 MS.gene35719:CDS 40.0%
GTACAATGACATCAGAAGTC+AGG + chr2.1:14087059-14087078 None:intergenic 40.0%
GTCAGAACTATGCAATCCAA+AGG - chr2.1:14086674-14086693 MS.gene35719:intron 40.0%
TAGTGCAAGCCTATTAGTAG+GGG - chr2.1:14086391-14086410 MS.gene35719:intron 40.0%
TCAACGAAAATGGTGACCTT+TGG - chr2.1:14087501-14087520 MS.gene35719:CDS 40.0%
TCTGATGTCATTGTACAAGC+AGG - chr2.1:14087063-14087082 MS.gene35719:intron 40.0%
TGGCTAAAAGGAGATAAAGC+TGG - chr2.1:14087428-14087447 MS.gene35719:intron 40.0%
TGTTAAAGCAGTGAGTGAAG+TGG - chr2.1:14087724-14087743 MS.gene35719:CDS 40.0%
TTGTCTTTGTCATGATCCAG+AGG + chr2.1:14086076-14086095 None:intergenic 40.0%
TTTGCAGAAGTTTACCGTCA+GGG + chr2.1:14087679-14087698 None:intergenic 40.0%
! TTTTGCCTCCAATATGTAGC+AGG + chr2.1:14087631-14087650 None:intergenic 40.0%
!! AGCAGGTATTGAATCCTTGT+TGG + chr2.1:14087614-14087633 None:intergenic 40.0%
!! CTAAGCAAGTACTGGCTAAA+AGG - chr2.1:14087416-14087435 MS.gene35719:intron 40.0%
!! GCAGGTATTGAATCCTTGTT+GGG + chr2.1:14087613-14087632 None:intergenic 40.0%
!! TTCGTTGACTCTCACATTGT+CGG + chr2.1:14087489-14087508 None:intergenic 40.0%
!!! CAATGTTTGGCTGGTTTTGT+TGG - chr2.1:14087032-14087051 MS.gene35719:CDS 40.0%
!!! TATTTTGGAACGGACTGTCT+TGG - chr2.1:14086209-14086228 MS.gene35719:CDS 40.0%
AAATCAGGGAAGCCTTGGAT+AGG + chr2.1:14086013-14086032 None:intergenic 45.0%
AAGAACAGTGGTTTCCTTGG+TGG + chr2.1:14087150-14087169 None:intergenic 45.0%
AATCAGGGAAGCCTTGGATA+GGG + chr2.1:14086012-14086031 None:intergenic 45.0%
AATCCTTCCATCAGCAACAC+CGG + chr2.1:14086188-14086207 None:intergenic 45.0%
ACGTAGACGTTGATGCAGAA+GGG - chr2.1:14086892-14086911 MS.gene35719:CDS 45.0%
AGAGGAACTTCAGACCATGA+TGG + chr2.1:14086058-14086077 None:intergenic 45.0%
AGCATTGCCTTCGATTCTCA+TGG - chr2.1:14086148-14086167 MS.gene35719:CDS 45.0%
AGGAGAAGGATGGTAAACTC+TGG - chr2.1:14087747-14087766 MS.gene35719:CDS 45.0%
ATATTTCGGGCTGATGAAGG+TGG - chr2.1:14086800-14086819 MS.gene35719:CDS 45.0%
CACAAGAACAGTGGTTTCCT+TGG + chr2.1:14087153-14087172 None:intergenic 45.0%
CATGTTTGTGAACCAAGTGC+AGG - chr2.1:14086117-14086136 MS.gene35719:CDS 45.0%
GAAGGAACCATCCTTGCTTA+AGG + chr2.1:14087204-14087223 None:intergenic 45.0%
GGGATTTCCTTAAGCAAGGA+TGG - chr2.1:14087194-14087213 MS.gene35719:intron 45.0%
GTCAGGGCTATACTTAACGA+CGG + chr2.1:14087663-14087682 None:intergenic 45.0%
GTCGTTAAGTATAGCCCTGA+CGG - chr2.1:14087662-14087681 MS.gene35719:CDS 45.0%
TAAAGCAGTGAGTGAAGTGG+AGG - chr2.1:14087727-14087746 MS.gene35719:CDS 45.0%
TACAATGGATAGCCACCCAA+AGG + chr2.1:14087520-14087539 None:intergenic 45.0%
TATTTCGGGCTGATGAAGGT+GGG - chr2.1:14086801-14086820 MS.gene35719:CDS 45.0%
TCAAGCTACCTATCCCAACA+AGG - chr2.1:14087597-14087616 MS.gene35719:CDS 45.0%
TCATACATGACGCTAACTCG+AGG - chr2.1:14085926-14085945 MS.gene35719:CDS 45.0%
TCTGTAGGCTAAGCAAGTAC+TGG - chr2.1:14087408-14087427 MS.gene35719:intron 45.0%
TGCATATTTCGGGCTGATGA+AGG - chr2.1:14086797-14086816 MS.gene35719:CDS 45.0%
TGGATAGGGCTTTGCTTGAA+GGG + chr2.1:14085998-14086017 None:intergenic 45.0%
TGGGATAGGTAGCTTGAGTA+TGG + chr2.1:14087594-14087613 None:intergenic 45.0%
TTCAAGCAAAGCCCTATCCA+AGG - chr2.1:14085998-14086017 MS.gene35719:CDS 45.0%
TTGGATAGGGCTTTGCTTGA+AGG + chr2.1:14085999-14086018 None:intergenic 45.0%
! ATCATGGTCTGAAGTTCCTC+TGG - chr2.1:14086057-14086076 MS.gene35719:CDS 45.0%
! CTCTTGTTTGCTCTCTACTG+TGG - chr2.1:14085965-14085984 MS.gene35719:CDS 45.0%
!! CGCACCTTATTTCTGAACAC+AGG - chr2.1:14086652-14086671 MS.gene35719:intron 45.0%
AGTGAAGTGGAGGAGAAGGA+TGG - chr2.1:14087737-14087756 MS.gene35719:CDS 50.0%
AGTGAGTGAAGTGGAGGAGA+AGG - chr2.1:14087733-14087752 MS.gene35719:CDS 50.0%
ATTTCGGGCTGATGAAGGTG+GGG - chr2.1:14086802-14086821 MS.gene35719:CDS 50.0%
CATCAGCAACACCGGTGTAA+GGG + chr2.1:14086180-14086199 None:intergenic 50.0%
CATTGTCGGCAAAACCAGGT+AGG + chr2.1:14087475-14087494 None:intergenic 50.0%
CCTCAAAATCAGGGAAGCCT+TGG + chr2.1:14086018-14086037 None:intergenic 50.0%
CGAAAATGGTGACCTTTGGG+TGG - chr2.1:14087505-14087524 MS.gene35719:CDS 50.0%
CTCACATTGTCGGCAAAACC+AGG + chr2.1:14087479-14087498 None:intergenic 50.0%
GAATCGAAGGCAATGCTCTC+CGG + chr2.1:14086145-14086164 None:intergenic 50.0%
GACGTAGACGTTGATGCAGA+AGG - chr2.1:14086891-14086910 MS.gene35719:CDS 50.0%
GATGTTGCGAAACCACCTTG+AGG + chr2.1:14086828-14086847 None:intergenic 50.0%
GGATAGGGCTTTGCTTGAAG+GGG + chr2.1:14085997-14086016 None:intergenic 50.0%
GGGTGGCTATCCATTGTAGA+AGG - chr2.1:14087522-14087541 MS.gene35719:CDS 50.0%
GTTGACACTGAGGACATCTG+CGG - chr2.1:14086720-14086739 MS.gene35719:intron 50.0%
TTGACACTGAGGACATCTGC+GGG - chr2.1:14086721-14086740 MS.gene35719:intron 50.0%
! AGAAGAGAAGCGTGACCTGT+TGG + chr2.1:14086257-14086276 None:intergenic 50.0%
! CCAAGGCTTCCCTGATTTTG+AGG - chr2.1:14086015-14086034 MS.gene35719:CDS 50.0%
! GAAGAGAAGCGTGACCTGTT+GGG + chr2.1:14086256-14086275 None:intergenic 50.0%
!! AGTGGTGTTGCAGATGCCTT+TGG + chr2.1:14086693-14086712 None:intergenic 50.0%
CCATCAGCAACACCGGTGTA+AGG + chr2.1:14086181-14086200 None:intergenic 55.0%
CGCAACATCGCTTGCAACTG+AGG - chr2.1:14086839-14086858 MS.gene35719:CDS 55.0%
GCCACGACCATGAGAATCGA+AGG + chr2.1:14086158-14086177 None:intergenic 55.0%
GCCTTCGATTCTCATGGTCG+TGG - chr2.1:14086154-14086173 MS.gene35719:CDS 55.0%
TCGCTTGCAACTGAGGCAGA+AGG - chr2.1:14086846-14086865 MS.gene35719:CDS 55.0%
TGTGAACCAAGTGCAGGGAC+CGG - chr2.1:14086123-14086142 MS.gene35719:CDS 55.0%
TTGCATACACCTCGCCCAAC+AGG - chr2.1:14086239-14086258 MS.gene35719:CDS 55.0%
!! ACACCGGTGTTGCTGATGGA+AGG - chr2.1:14086182-14086201 MS.gene35719:CDS 55.0%
!! CCTTACACCGGTGTTGCTGA+TGG - chr2.1:14086178-14086197 MS.gene35719:CDS 55.0%
ACACTGAGGACATCTGCGGG+AGG - chr2.1:14086724-14086743 MS.gene35719:intron 60.0%
ATGAAGGTGGGGCCTCAAGG+TGG - chr2.1:14086813-14086832 MS.gene35719:CDS 60.0%
CATGGTCGTGGCCCTTACAC+CGG - chr2.1:14086166-14086185 MS.gene35719:CDS 60.0%
CTGATGAAGGTGGGGCCTCA+AGG - chr2.1:14086810-14086829 MS.gene35719:CDS 60.0%
GACATCTGCGGGAGGCCTTT+GGG - chr2.1:14086732-14086751 MS.gene35719:intron 60.0%
GAAGCGTGACCTGTTGGGCG+AGG + chr2.1:14086251-14086270 None:intergenic 65.0%
GGACATCTGCGGGAGGCCTT+TGG - chr2.1:14086731-14086750 MS.gene35719:intron 65.0%
TGCTCTCCGGTCCCTGCACT+TGG + chr2.1:14086132-14086151 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 14085923 14087817 14085923 ID=MS.gene35719
chr2.1 mRNA 14085923 14087817 14085923 ID=MS.gene35719.t1;Parent=MS.gene35719
chr2.1 exon 14087480 14087817 14087480 ID=MS.gene35719.t1.exon1;Parent=MS.gene35719.t1
chr2.1 CDS 14087480 14087817 14087480 ID=cds.MS.gene35719.t1;Parent=MS.gene35719.t1
chr2.1 exon 14086785 14087066 14086785 ID=MS.gene35719.t1.exon2;Parent=MS.gene35719.t1
chr2.1 CDS 14086785 14087066 14086785 ID=cds.MS.gene35719.t1;Parent=MS.gene35719.t1
chr2.1 exon 14086488 14086655 14086488 ID=MS.gene35719.t1.exon3;Parent=MS.gene35719.t1
chr2.1 CDS 14086488 14086655 14086488 ID=cds.MS.gene35719.t1;Parent=MS.gene35719.t1
chr2.1 exon 14085923 14086325 14085923 ID=MS.gene35719.t1.exon4;Parent=MS.gene35719.t1
chr2.1 CDS 14085923 14086325 14085923 ID=cds.MS.gene35719.t1;Parent=MS.gene35719.t1
Gene Sequence

>MS.gene35719

ATGTCATACATGACGCTAACTCGAGGACTCGCTATTGTAATTCTCTTGTTTGCTCTCTACTGTGGATTAGACCCCTTCAAGCAAAGCCCTATCCAAGGCTTCCCTGATTTTGAGGTGCACAAAATTAACTTGCCATCATGGTCTGAAGTTCCTCTGGATCATGACAAAGACAACTTGTTGAAGAAATCGGAGCTCATGTTTGTGAACCAAGTGCAGGGACCGGAGAGCATTGCCTTCGATTCTCATGGTCGTGGCCCTTACACCGGTGTTGCTGATGGAAGGATTTTATTTTGGAACGGACTGTCTTGGATTGATTTTGCATACACCTCGCCCAACAGGTCACGCTTCTCTTCTTTCCTTATAACTTTTCCAACTTAAATGCTTAGTACAAGATAATTTTGTGATTTTGTTATCTTTCTGCATTACTGTCAATAGTTATCAAATTCATAGGTTATAGTCATATTCTAATAGTGCAAGCCTATTAGTAGGGGATATATGTGGCTAAAAGCTACTATAATTTCTAACTTAATGTTGGAAAACTGCTTCATGCAAACTTTTTAGCTCATACGCTCATACTATTAAAGATTTTATGCTCTTGCACCGATCTGTGAAAAAGTTACAGAAAATATGTGTGAAATTCTGCTACATTGCATCTGAGGAAGACTTTTCTTAAGATGAATGGATAATTTGTGGCAATGTAACTTTGCGATATCGTGTGATAATATTTCTCGCACCTTATTTCTGAACACAGGTCAGAACTATGCAATCCAAAGGCATCTGCAACACCACTTAGTTATGTTGACACTGAGGACATCTGCGGGAGGCCTTTGGGACTAAGATTTGACAAGAAAACTGGTGATTTATACATAGCAGATGCATATTTCGGGCTGATGAAGGTGGGGCCTCAAGGTGGTTTCGCAACATCGCTTGCAACTGAGGCAGAAGGAGTGCCGTTTAGATTTACTAATGACGTAGACGTTGATGCAGAAGGGAACGTTTACTTCACAGATAGCAGCACTAAATATCAGAGAAGGTTGTTAGTAATATTTTTTCTTGTTAACACTTCACCTTTGAAATTATCAACATTTTAAATATGAGAAATAGCAAAACAATGTTTGGCTGGTTTTGTTGGTCCTGACTTCTGATGTCATTGTACAAGCAGGAATTTCATTCAGTTGATACTTTCTGGTGATAATAATGGAAGGGTTTTGAAATACAATTCTGCCACCAAGGAAACCACTGTTCTTGTGAGAAATGTTCAATTTCCAAATGGGATTTCCTTAAGCAAGGATGGTTCCTTCTTTGTATTTTCAGAAGGAGTAATTGGAAGGTGTGTGAATAATCAACATAACCCTTTTTTCGTCTAATAACGATACACTAATGATGCAAACAAGTTTTACAAAGACGTCCAACATATTTTGCCATTCTGACACAATATGTTTAGAAAGTAATTTGTTTGTAAAGTTATTAATCAAGTCCGTTTTTTCTGTAGGCTAAGCAAGTACTGGCTAAAAGGAGATAAAGCTGGAACTTTAGAAATCTTAGCAATCCTACCTGGTTTTGCCGACAATGTGAGAGTCAACGAAAATGGTGACCTTTGGGTGGCTATCCATTGTAGAAGGTATATGTACAGTTACATAAATGCAATCTACCCAAAGATTAGAAAAGCCATACTCAAGCTACCTATCCCAACAAGGATTCAATACCTGCTACATATTGGAGGCAAAATGCATGCAGCCGTCGTTAAGTATAGCCCTGACGGTAAACTTCTGCAAATATTGGAGGACAATGAGGGAAAAGTTGTTAAAGCAGTGAGTGAAGTGGAGGAGAAGGATGGTAAACTCTGGATAGGAAGTGTTTTGATGCCTTTCATTGCGGTTTATCATTTGACATGA
Protein sequence

>MS.gene35719.t1

MSYMTLTRGLAIVILLFALYCGLDPFKQSPIQGFPDFEVHKINLPSWSEVPLDHDKDNLLKKSELMFVNQVQGPESIAFDSHGRGPYTGVADGRILFWNGLSWIDFAYTSPNRSELCNPKASATPLSYVDTEDICGRPLGLRFDKKTGDLYIADAYFGLMKVGPQGGFATSLATEAEGVPFRFTNDVDVDAEGNVYFTDSSTKYQRRNFIQLILSGDNNGRVLKYNSATKETTVLVRNVQFPNGISLSKDGSFFVFSEGVIGRLSKYWLKGDKAGTLEILAILPGFADNVRVNENGDLWVAIHCRRYMYSYINAIYPKIRKAILKLPIPTRIQYLLHIGGKMHAAVVKYSPDGKLLQILEDNEGKVVKAVSEVEEKDGKLWIGSVLMPFIAVYHLT