Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017353.t1 | RHN60700.1 | 98.9 | 178 | 2 | 0 | 1 | 178 | 4 | 181 | 5.60E-51 | 210.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017353.t1 | Q9H6L4 | 62.1 | 58 | 22 | 0 | 19 | 76 | 13 | 70 | 1.8e-13 | 77.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017353.t1 | A0A396I501 | 98.9 | 178 | 2 | 0 | 1 | 178 | 4 | 181 | 4.0e-51 | 210.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene017353 | MS.gene00478 | PPI |
| MS.gene00474 | MS.gene017353 | PPI |
| MS.gene017353 | MS.gene044085 | PPI |
| MS.gene017353 | MS.gene24450 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017353.t1 | MTR_5g077040 | 97.753 | 178 | 4 | 0 | 1 | 178 | 1 | 178 | 1.70e-127 | 355 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017353.t1 | AT5G37290 | 73.184 | 179 | 46 | 1 | 1 | 177 | 1 | 179 | 1.50e-100 | 287 |
| MS.gene017353.t1 | AT5G37290 | 73.184 | 179 | 46 | 1 | 1 | 177 | 1 | 179 | 1.50e-100 | 287 |
| MS.gene017353.t1 | AT5G37290 | 72.951 | 122 | 33 | 0 | 1 | 122 | 1 | 122 | 3.48e-67 | 201 |
Find 42 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTGTTGCCTTTGGTTAT+TGG | 0.174361 | 5.1:-60067811 | None:intergenic |
| AATGAAAAGATTATAGAATT+TGG | 0.211644 | 5.1:+60068346 | MS.gene017353:CDS |
| AGAAATTAAAGAGAAGATTT+TGG | 0.229064 | 5.1:+60068121 | MS.gene017353:intron |
| ATAATTGCAGCTAAATGTTT+TGG | 0.267268 | 5.1:+60068294 | MS.gene017353:intron |
| ATCCTTACAGTGTTCCTAAC+TGG | 0.273581 | 5.1:-60068559 | None:intergenic |
| CGGTCCGCTCTTGTTGCCTT+TGG | 0.333981 | 5.1:-60067818 | None:intergenic |
| GTTAAAATTGTAAGGATCAT+AGG | 0.342362 | 5.1:-60068162 | None:intergenic |
| TAGTGTGAGCTTCAGTAATC+TGG | 0.391697 | 5.1:+60068936 | MS.gene017353:CDS |
| CAAGAGCGGACCGGAAGATA+TGG | 0.397319 | 5.1:+60067828 | MS.gene017353:CDS |
| CACCTGAAGGTATTGCAATC+TGG | 0.406154 | 5.1:-60067856 | None:intergenic |
| AATGTTTATCAAGCCCAGTT+AGG | 0.407380 | 5.1:+60068545 | MS.gene017353:CDS |
| CGCAAGAAGTTAAAATTGTA+AGG | 0.419936 | 5.1:-60068170 | None:intergenic |
| CCTTTGGTTATTGGTGAACA+TGG | 0.442442 | 5.1:-60067802 | None:intergenic |
| TTGATGACATCAATAACTTC+AGG | 0.448267 | 5.1:-60068889 | None:intergenic |
| TTCCAGATTGCAATACCTTC+AGG | 0.449232 | 5.1:+60067854 | MS.gene017353:CDS |
| GATAAACATTGAATGATAAG+AGG | 0.452418 | 5.1:-60068532 | None:intergenic |
| TCCTTACAGTGTTCCTAACT+GGG | 0.459088 | 5.1:-60068558 | None:intergenic |
| GCTACGATTGTAGCGTTGGC+TGG | 0.464215 | 5.1:-60068493 | None:intergenic |
| TTCTTGACAAACATCTATCA+AGG | 0.487090 | 5.1:+60068968 | MS.gene017353:CDS |
| TCCAGCTACGATTGTAGCGT+TGG | 0.517915 | 5.1:-60068497 | None:intergenic |
| TCTGGAAGTACCATATCTTC+CGG | 0.518139 | 5.1:-60067838 | None:intergenic |
| GCCAACGCTACAATCGTAGC+TGG | 0.526233 | 5.1:+60068496 | MS.gene017353:CDS |
| GTAAGGATCATAGGCAAAGT+TGG | 0.532617 | 5.1:-60068153 | None:intergenic |
| ATCAGGTAAATTATGCACTC+GGG | 0.532999 | 5.1:+60068818 | MS.gene017353:intron |
| TTATAGAATTTGGTGTCGGA+GGG | 0.539943 | 5.1:+60068356 | MS.gene017353:CDS |
| ATTATAGAATTTGGTGTCGG+AGG | 0.541324 | 5.1:+60068355 | MS.gene017353:CDS |
| CAAAGGCAACAAGAGCGGAC+CGG | 0.544877 | 5.1:+60067819 | MS.gene017353:CDS |
| GGGATCTGCAATTGTTGTGC+TGG | 0.546095 | 5.1:+60068376 | MS.gene017353:CDS |
| GCTACAATCGTAGCTGGAGC+TGG | 0.546747 | 5.1:+60068502 | MS.gene017353:CDS |
| GATCAGGTAAATTATGCACT+CGG | 0.549275 | 5.1:+60068817 | MS.gene017353:intron |
| ACAATCGTAGCTGGAGCTGG+TGG | 0.549647 | 5.1:+60068505 | MS.gene017353:CDS |
| TAATGAATCTAACAAGGAAG+AGG | 0.567771 | 5.1:+60068858 | MS.gene017353:CDS |
| TGGGTTCAGTTACGCAGTCA+AGG | 0.570036 | 5.1:-60068324 | None:intergenic |
| AAGATTATAGAATTTGGTGT+CGG | 0.575863 | 5.1:+60068352 | MS.gene017353:CDS |
| TTTCAGAATTCATCAGAGGA+TGG | 0.593173 | 5.1:+60067981 | MS.gene017353:CDS |
| CAATCGTAGCTGGAGCTGGT+GGG | 0.597842 | 5.1:+60068506 | MS.gene017353:CDS |
| CATATGTAATGAATCTAACA+AGG | 0.609568 | 5.1:+60068852 | MS.gene017353:CDS |
| AAGTTATTGATGTCATCAAG+AGG | 0.636725 | 5.1:+60068893 | MS.gene017353:CDS |
| TCAGTTTCAGAATTCATCAG+AGG | 0.641335 | 5.1:+60067977 | MS.gene017353:CDS |
| CCATGTTCACCAATAACCAA+AGG | 0.659452 | 5.1:+60067802 | None:intergenic |
| ATAACCAAAGGCAACAAGAG+CGG | 0.667897 | 5.1:+60067814 | MS.gene017353:CDS |
| TCAGGTAAATTATGCACTCG+GGG | 0.677160 | 5.1:+60068819 | MS.gene017353:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAAAAGATTATAGAATT+TGG | + | chr5.1:60068346-60068365 | MS.gene017353:CDS | 15.0% |
| !!! | AAATTCTATAATCTTTTCAT+TGG | - | chr5.1:60068347-60068366 | None:intergenic | 15.0% |
| !!! | AATTCTATAATCTTTTCATT+GGG | - | chr5.1:60068346-60068365 | None:intergenic | 15.0% |
| !!! | AGTTGTTTATCAAATTTTTT+GGG | - | chr5.1:60068784-60068803 | None:intergenic | 15.0% |
| !!! | TAGTTGTTTATCAAATTTTT+TGG | - | chr5.1:60068785-60068804 | None:intergenic | 15.0% |
| !! | AAACAAACGTTATTCATATA+AGG | - | chr5.1:60067943-60067962 | None:intergenic | 20.0% |
| !! | AATGCAAATTAAGTAGTATA+AGG | - | chr5.1:60068657-60068676 | None:intergenic | 20.0% |
| !! | ATGCAAATTAAGTAGTATAA+GGG | - | chr5.1:60068656-60068675 | None:intergenic | 20.0% |
| !!! | AGAAATTAAAGAGAAGATTT+TGG | + | chr5.1:60068121-60068140 | MS.gene017353:intron | 20.0% |
| !!! | GTTGTTTATCAAATTTTTTG+GGG | - | chr5.1:60068783-60068802 | None:intergenic | 20.0% |
| ! | ATATGAATAACGTTTGTTTC+AGG | + | chr5.1:60067944-60067963 | MS.gene017353:intron | 25.0% |
| ! | CATATGTAATGAATCTAACA+AGG | + | chr5.1:60068852-60068871 | MS.gene017353:CDS | 25.0% |
| ! | GATAAACATTGAATGATAAG+AGG | - | chr5.1:60068535-60068554 | None:intergenic | 25.0% |
| ! | GTTAAAATTGTAAGGATCAT+AGG | - | chr5.1:60068165-60068184 | None:intergenic | 25.0% |
| ! | TTATTGCAAAGACAATTAGT+GGG | - | chr5.1:60068207-60068226 | None:intergenic | 25.0% |
| !! | ATAATTGCAGCTAAATGTTT+TGG | + | chr5.1:60068294-60068313 | MS.gene017353:intron | 25.0% |
| !!! | AAGATTATAGAATTTGGTGT+CGG | + | chr5.1:60068352-60068371 | MS.gene017353:CDS | 25.0% |
| !!! | AATTTTTTGGGGATCTTTTA+TGG | - | chr5.1:60068772-60068791 | None:intergenic | 25.0% |
| AAGTTATTGATGTCATCAAG+AGG | + | chr5.1:60068893-60068912 | MS.gene017353:CDS | 30.0% | |
| ACTAACGTATTTATCTGATC+AGG | + | chr5.1:60068801-60068820 | MS.gene017353:intron | 30.0% | |
| AGAAATCACAATACTGTGAA+AGG | - | chr5.1:60068419-60068438 | None:intergenic | 30.0% | |
| ATAAAGAATCCTACCAACAA+AGG | - | chr5.1:60068056-60068075 | None:intergenic | 30.0% | |
| ATAACGTTTGTTTCAGGAAT+TGG | + | chr5.1:60067950-60067969 | MS.gene017353:intron | 30.0% | |
| CGCAAGAAGTTAAAATTGTA+AGG | - | chr5.1:60068173-60068192 | None:intergenic | 30.0% | |
| CTTATTGCAAAGACAATTAG+TGG | - | chr5.1:60068208-60068227 | None:intergenic | 30.0% | |
| TAATGAATCTAACAAGGAAG+AGG | + | chr5.1:60068858-60068877 | MS.gene017353:CDS | 30.0% | |
| TTCTTGACAAACATCTATCA+AGG | + | chr5.1:60068968-60068987 | MS.gene017353:CDS | 30.0% | |
| ! | TTGATGACATCAATAACTTC+AGG | - | chr5.1:60068892-60068911 | None:intergenic | 30.0% |
| ! | TTTACCATCTCCTTATTTGT+TGG | + | chr5.1:60068077-60068096 | MS.gene017353:intron | 30.0% |
| !! | ACAAGCAATTTTGAGAAATC+AGG | - | chr5.1:60068687-60068706 | None:intergenic | 30.0% |
| !! | AGAGAAGATTTTGGCAAATT+TGG | + | chr5.1:60068130-60068149 | MS.gene017353:CDS | 30.0% |
| !!! | GTTTGCTTGTTGATTTTGTA+AGG | + | chr5.1:60068586-60068605 | MS.gene017353:intron | 30.0% |
| AACACCAACAAATAAGGAGA+TGG | - | chr5.1:60068084-60068103 | None:intergenic | 35.0% | |
| AATCTTTCAAGCACCTTTGT+TGG | + | chr5.1:60068040-60068059 | MS.gene017353:intron | 35.0% | |
| AATGTTTATCAAGCCCAGTT+AGG | + | chr5.1:60068545-60068564 | MS.gene017353:CDS | 35.0% | |
| ACGAGAAACACCAACAAATA+AGG | - | chr5.1:60068090-60068109 | None:intergenic | 35.0% | |
| ATCAGGTAAATTATGCACTC+GGG | + | chr5.1:60068818-60068837 | MS.gene017353:intron | 35.0% | |
| GAAGAAGAAAAAACACCTGA+AGG | - | chr5.1:60067872-60067891 | None:intergenic | 35.0% | |
| GATCAGGTAAATTATGCACT+CGG | + | chr5.1:60068817-60068836 | MS.gene017353:intron | 35.0% | |
| TCAGTTTCAGAATTCATCAG+AGG | + | chr5.1:60067977-60067996 | MS.gene017353:CDS | 35.0% | |
| TTTCAGAATTCATCAGAGGA+TGG | + | chr5.1:60067981-60068000 | MS.gene017353:CDS | 35.0% | |
| !! | ATTATAGAATTTGGTGTCGG+AGG | + | chr5.1:60068355-60068374 | MS.gene017353:CDS | 35.0% |
| !! | TTATAGAATTTGGTGTCGGA+GGG | + | chr5.1:60068356-60068375 | MS.gene017353:CDS | 35.0% |
| ATAACCAAAGGCAACAAGAG+CGG | + | chr5.1:60067814-60067833 | MS.gene017353:CDS | 40.0% | |
| ATCCTTACAGTGTTCCTAAC+TGG | - | chr5.1:60068562-60068581 | None:intergenic | 40.0% | |
| CTCTTGTTGCCTTTGGTTAT+TGG | - | chr5.1:60067814-60067833 | None:intergenic | 40.0% | |
| GTAAGGATCATAGGCAAAGT+TGG | - | chr5.1:60068156-60068175 | None:intergenic | 40.0% | |
| TAGTGTGAGCTTCAGTAATC+TGG | + | chr5.1:60068936-60068955 | MS.gene017353:CDS | 40.0% | |
| TCAGGTAAATTATGCACTCG+GGG | + | chr5.1:60068819-60068838 | MS.gene017353:intron | 40.0% | |
| TCCTTACAGTGTTCCTAACT+GGG | - | chr5.1:60068561-60068580 | None:intergenic | 40.0% | |
| TTCCAGATTGCAATACCTTC+AGG | + | chr5.1:60067854-60067873 | MS.gene017353:CDS | 40.0% | |
| TTTCAAGCACCTTTGTTGGT+AGG | + | chr5.1:60068044-60068063 | MS.gene017353:intron | 40.0% | |
| !! | TCTGGAAGTACCATATCTTC+CGG | - | chr5.1:60067841-60067860 | None:intergenic | 40.0% |
| !!! | CAATTTTAACTTCTTGCGCC+AGG | + | chr5.1:60068175-60068194 | MS.gene017353:CDS | 40.0% |
| TTAACTTCTTGCGCCAGGTA+TGG | + | chr5.1:60068180-60068199 | MS.gene017353:intron | 45.0% | |
| ! | ACAATTAGTGGGTCCATACC+TGG | - | chr5.1:60068196-60068215 | None:intergenic | 45.0% |
| !! | CACCTGAAGGTATTGCAATC+TGG | - | chr5.1:60067859-60067878 | None:intergenic | 45.0% |
| GCCCAGTTAGGAACACTGTA+AGG | + | chr5.1:60068557-60068576 | MS.gene017353:intron | 50.0% | |
| GGGATCTGCAATTGTTGTGC+TGG | + | chr5.1:60068376-60068395 | MS.gene017353:CDS | 50.0% | |
| TCCAGCTACGATTGTAGCGT+TGG | - | chr5.1:60068500-60068519 | None:intergenic | 50.0% | |
| TGGGTTCAGTTACGCAGTCA+AGG | - | chr5.1:60068327-60068346 | None:intergenic | 50.0% | |
| ACAATCGTAGCTGGAGCTGG+TGG | + | chr5.1:60068505-60068524 | MS.gene017353:CDS | 55.0% | |
| CAAAGGCAACAAGAGCGGAC+CGG | + | chr5.1:60067819-60067838 | MS.gene017353:CDS | 55.0% | |
| CAAGAGCGGACCGGAAGATA+TGG | + | chr5.1:60067828-60067847 | MS.gene017353:CDS | 55.0% | |
| CAATCGTAGCTGGAGCTGGT+GGG | + | chr5.1:60068506-60068525 | MS.gene017353:CDS | 55.0% | |
| GCCAACGCTACAATCGTAGC+TGG | + | chr5.1:60068496-60068515 | MS.gene017353:CDS | 55.0% | |
| GCTACAATCGTAGCTGGAGC+TGG | + | chr5.1:60068502-60068521 | MS.gene017353:CDS | 55.0% | |
| !! | GCTACGATTGTAGCGTTGGC+TGG | - | chr5.1:60068496-60068515 | None:intergenic | 55.0% |
| CGGTCCGCTCTTGTTGCCTT+TGG | - | chr5.1:60067821-60067840 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 60067804 | 60068999 | 60067804 | ID=MS.gene017353 |
| chr5.1 | mRNA | 60067804 | 60068999 | 60067804 | ID=MS.gene017353.t1;Parent=MS.gene017353 |
| chr5.1 | exon | 60067804 | 60067875 | 60067804 | ID=MS.gene017353.t1.exon1;Parent=MS.gene017353.t1 |
| chr5.1 | CDS | 60067804 | 60067875 | 60067804 | ID=cds.MS.gene017353.t1;Parent=MS.gene017353.t1 |
| chr5.1 | exon | 60067966 | 60068002 | 60067966 | ID=MS.gene017353.t1.exon2;Parent=MS.gene017353.t1 |
| chr5.1 | CDS | 60067966 | 60068002 | 60067966 | ID=cds.MS.gene017353.t1;Parent=MS.gene017353.t1 |
| chr5.1 | exon | 60068123 | 60068196 | 60068123 | ID=MS.gene017353.t1.exon3;Parent=MS.gene017353.t1 |
| chr5.1 | CDS | 60068123 | 60068196 | 60068123 | ID=cds.MS.gene017353.t1;Parent=MS.gene017353.t1 |
| chr5.1 | exon | 60068304 | 60068397 | 60068304 | ID=MS.gene017353.t1.exon4;Parent=MS.gene017353.t1 |
| chr5.1 | CDS | 60068304 | 60068397 | 60068304 | ID=cds.MS.gene017353.t1;Parent=MS.gene017353.t1 |
| chr5.1 | exon | 60068491 | 60068573 | 60068491 | ID=MS.gene017353.t1.exon5;Parent=MS.gene017353.t1 |
| chr5.1 | CDS | 60068491 | 60068573 | 60068491 | ID=cds.MS.gene017353.t1;Parent=MS.gene017353.t1 |
| chr5.1 | exon | 60068823 | 60068999 | 60068823 | ID=MS.gene017353.t1.exon6;Parent=MS.gene017353.t1 |
| chr5.1 | CDS | 60068823 | 60068999 | 60068823 | ID=cds.MS.gene017353.t1;Parent=MS.gene017353.t1 |
| Gene Sequence |
| Protein sequence |
