AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01773

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01773.t1 MTR_2g012510 98.747 399 4 1 1 399 69 466 0.0 812
MS.gene01773.t1 MTR_4g090525 70.750 400 102 3 1 399 65 450 0.0 560
MS.gene01773.t1 MTR_8g464260 67.725 378 119 1 22 399 4 378 0.0 530
MS.gene01773.t1 MTR_4g094672 39.373 287 137 6 80 357 1 259 7.28e-60 195
MS.gene01773.t1 MTR_2g011680 34.097 393 207 16 19 390 44 405 5.48e-41 150
MS.gene01773.t1 MTR_3g109970 76.667 60 13 1 76 134 6 65 2.58e-23 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01773.t1 AT5G54390 64.343 373 125 2 27 399 9 373 2.48e-170 481
MS.gene01773.t1 AT5G63980 44.789 355 168 6 24 378 3 329 1.71e-94 287
MS.gene01773.t1 AT5G09290 42.162 370 173 8 24 393 3 331 2.18e-84 261
MS.gene01773.t1 AT5G63990 40.282 355 181 9 24 378 3 326 5.74e-78 245
MS.gene01773.t1 AT5G64000 41.570 344 170 7 24 367 3 315 1.52e-73 233
MS.gene01773.t1 AT5G63990 38.006 321 169 8 24 344 3 293 8.29e-64 206
MS.gene01773.t1 AT5G64000 40.816 294 146 6 74 367 7 272 1.35e-59 196
MS.gene01773.t1 AT4G05090 31.486 397 198 14 23 393 45 393 3.52e-39 144
MS.gene01773.t1 AT4G05090 30.311 386 185 12 23 393 45 361 6.93e-35 132

Find 91 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTCCGCATAGGGTAATT+TGG 0.135632 2.3:+71555824 None:intergenic
ACCATGCTGTATACTCTTAA+TGG 0.160691 2.3:+71554668 None:intergenic
GAATTTCCAAGGTCTGATTT+TGG 0.209004 2.3:+71556016 None:intergenic
TTGTGTGCTATATGCTAAAA+AGG 0.214002 2.3:-71555723 MS.gene01773:CDS
GAAGCAGTGCTTGGTGTTCT+TGG 0.245513 2.3:-71555851 MS.gene01773:CDS
GTGGTCCCTCTGGAAGATTT+TGG 0.265186 2.3:-71555955 MS.gene01773:CDS
CGCGGTATAATTGCTTGTTC+TGG 0.292721 2.3:-71554458 MS.gene01773:CDS
TCAAAAGGATTATATTTAGA+AGG 0.294817 2.3:-71554488 MS.gene01773:CDS
CTGAACCTGATGATTGGTTT+TGG 0.301141 2.3:+71557247 None:intergenic
CTTGAATTGGCCTTCTCAAC+TGG 0.304582 2.3:+71555578 None:intergenic
AATTCTCCTGTTACTGTTGC+TGG 0.304788 2.3:-71557212 MS.gene01773:intron
GACTAACCAGCAACAGTAAC+AGG 0.314879 2.3:+71557206 None:intergenic
CAATCATCAGGTTCAGTCTA+AGG 0.320159 2.3:-71557240 MS.gene01773:CDS
TTGATGCTGTTGATGCTAGC+TGG 0.336054 2.3:-71554412 MS.gene01773:CDS
AAAATTTGTATTGCATGGTT+TGG 0.340323 2.3:+71555644 None:intergenic
TAGTTCAGATGCATTAGATT+TGG 0.341068 2.3:+71556105 None:intergenic
GTTATCAACACCGGTATCAT+AGG 0.364679 2.3:-71555793 MS.gene01773:CDS
TGAAGAATATTCCAAGGAAT+TGG 0.368050 2.3:-71557336 MS.gene01773:CDS
CAGGTTACAAGGAGAAAATA+TGG 0.379484 2.3:-71554589 MS.gene01773:CDS
TGGGGCTGTTCTAGCCTTTG+AGG 0.398273 2.3:-71554392 None:intergenic
CTAGCTGAAGCGCCTCGATT+TGG 0.410609 2.3:-71556049 MS.gene01773:CDS
CTTAGACTGAACCTGATGAT+TGG 0.412831 2.3:+71557241 None:intergenic
GATTTCCAAAACCAATCATC+AGG 0.419970 2.3:-71557252 MS.gene01773:CDS
CTCCTCCAACTTCTGAAAGT+TGG 0.420854 2.3:-71555754 MS.gene01773:CDS
AGTAAAAGAATGGCTTGAAT+TGG 0.437244 2.3:+71555565 None:intergenic
AACTCAACTGGTGGTCCCTC+TGG 0.439010 2.3:-71555965 MS.gene01773:CDS
TAGCATTGACAATCCAGCTT+TGG 0.439179 2.3:-71555615 MS.gene01773:CDS
TGATGCTGTTGATGCTAGCT+GGG 0.442043 2.3:-71554411 MS.gene01773:CDS
TGTTCTGGAGCCTCATTACA+TGG 0.442558 2.3:-71554443 MS.gene01773:CDS
ATGGTAAAATATGCAGCAAT+AGG 0.443434 2.3:-71554650 MS.gene01773:CDS
TTGTTCCAACTTTCAGAAGT+TGG 0.452682 2.3:+71555749 None:intergenic
TTGCTCACAGTGTTGGTCTC+AGG 0.458647 2.3:-71555535 MS.gene01773:intron
ATAAGTCGCTGCAACTCAAC+TGG 0.460187 2.3:-71555977 MS.gene01773:CDS
TTAACCATTCCTTCCGCATA+GGG 0.463845 2.3:+71555816 None:intergenic
AACGGCAACATCCAATTCCT+TGG 0.469756 2.3:+71557325 None:intergenic
ATTTGCAAGGGCAGGTTACA+AGG 0.471911 2.3:-71554600 MS.gene01773:CDS
GTGTCCAAGCAGTTGTCAGC+TGG 0.480697 2.3:-71556195 MS.gene01773:CDS
TTCACTTTGTCAGAGAGTGC+AGG 0.484393 2.3:-71557282 MS.gene01773:CDS
TTCATGAAATTTGCAAGGGC+AGG 0.487309 2.3:-71554608 MS.gene01773:CDS
GATCCTGTCGATGGTACACT+CGG 0.498632 2.3:-71555926 MS.gene01773:CDS
TGCCGTTAGAGCAGTGCAAA+TGG 0.510068 2.3:-71557309 MS.gene01773:CDS
AGCCATTTGCACTGCTCTAA+CGG 0.525970 2.3:+71557307 None:intergenic
ATATCCAGCTGACAACTGCT+TGG 0.526105 2.3:+71556191 None:intergenic
GGTACACTCGGGTTTGTTCG+TGG 0.527148 2.3:-71555914 MS.gene01773:CDS
CTTAACCATTCCTTCCGCAT+AGG 0.528534 2.3:+71555815 None:intergenic
CAAGTCCTGCAGTAAAAGAA+TGG 0.531366 2.3:+71555555 None:intergenic
TGCAAATTTCATGAATACCT+CGG 0.535090 2.3:+71554616 None:intergenic
GGTGGAGTGGTAACAGATGC+TGG 0.542538 2.3:-71554530 MS.gene01773:CDS
TTCACCTATCATGGAGGATG+AGG 0.542664 2.3:-71557408 None:intergenic
GTTATCATCATACAAGAAGC+TGG 0.542954 2.3:-71554551 MS.gene01773:CDS
TGTCCCCTAGACTTCTCAAA+AGG 0.543780 2.3:-71554503 MS.gene01773:CDS
TCGATACAATCCTATGATAC+CGG 0.549696 2.3:+71555783 None:intergenic
GCAGGACTTGCTCACAGTGT+TGG 0.551173 2.3:-71555542 MS.gene01773:CDS
AAATATGCAGCAATAGGTCG+TGG 0.554833 2.3:-71554644 MS.gene01773:CDS
CGAGAACTTCTGAATTTCCA+AGG 0.556247 2.3:+71556005 None:intergenic
TGGGTATTGGATCCTGTCGA+TGG 0.556759 2.3:-71555935 MS.gene01773:CDS
AAAATATGGGATCATGCTGC+AGG 0.556967 2.3:-71554575 MS.gene01773:CDS
ATCTTGTTCACATGTAGCAA+TGG 0.560010 2.3:+71555677 None:intergenic
AGGTTACAAGGAGAAAATAT+GGG 0.562448 2.3:-71554588 MS.gene01773:CDS
AACCCGAGTGTACCATCGAC+AGG 0.563225 2.3:+71555923 None:intergenic
AATGATCTTTCCATGTAATG+AGG 0.563576 2.3:+71554433 None:intergenic
CAAATCGAGGCGCTTCAGCT+AGG 0.564002 2.3:+71556050 None:intergenic
GCTCTAGCGCTCGTAGAGGA+TGG 0.564050 2.3:-71555875 MS.gene01773:CDS
GAGGATGGAGAAGCAGTGCT+TGG 0.565663 2.3:-71555860 MS.gene01773:CDS
ATTACCCTATGCGGAAGGAA+TGG 0.571169 2.3:-71555820 MS.gene01773:CDS
AAAAGGGTACTGGAAAGGCT+TGG 0.572766 2.3:-71555706 MS.gene01773:CDS
TGGATATTATCTGAGTGTCT+AGG 0.576919 2.3:-71556175 MS.gene01773:CDS
AGATGATGAAGAATATTCCA+AGG 0.585121 2.3:-71557342 MS.gene01773:CDS
AGGTATTCATGAAATTTGCA+AGG 0.586091 2.3:-71554613 MS.gene01773:CDS
TTCCAACTTTCAGAAGTTGG+AGG 0.587710 2.3:+71555752 None:intergenic
ACTTCTGAAAGTTGGAACAA+AGG 0.591206 2.3:-71555746 MS.gene01773:CDS
GGTATTCATGAAATTTGCAA+GGG 0.607340 2.3:-71554612 MS.gene01773:CDS
CAGAAGCCAAAATCAGACCT+TGG 0.608552 2.3:-71556022 MS.gene01773:CDS
GGCTTCTGAACACCAAATCG+AGG 0.616592 2.3:+71556037 None:intergenic
TCCAAATTACCCTATGCGGA+AGG 0.618727 2.3:-71555825 MS.gene01773:CDS
CATACAAGAAGCTGGTGGAG+TGG 0.635866 2.3:-71554543 MS.gene01773:CDS
GATGCTGTTGATGCTAGCTG+GGG 0.654425 2.3:-71554410 MS.gene01773:CDS
AATAGGTCGTGGAGATGCCG+AGG 0.654938 2.3:-71554633 MS.gene01773:CDS
AATGGTTAAGTTATCAACAC+CGG 0.658343 2.3:-71555802 MS.gene01773:CDS
ATCTGAACTATTAGACTCCG+TGG 0.662295 2.3:-71556092 MS.gene01773:CDS
TATTTAGAAGGCTTAGATCG+CGG 0.665083 2.3:-71554476 MS.gene01773:CDS
AGTCGCTGCAACTCAACTGG+TGG 0.674152 2.3:-71555974 MS.gene01773:CDS
AATACCCAAAATCTTCCAGA+GGG 0.675463 2.3:+71555950 None:intergenic
GCTGTCCAAATTACCCTATG+CGG 0.675859 2.3:-71555829 MS.gene01773:CDS
ATCATCATACAAGAAGCTGG+TGG 0.677049 2.3:-71554548 MS.gene01773:CDS
ACCATTAAGAGTATACAGCA+TGG 0.679685 2.3:-71554669 MS.gene01773:CDS
ATCCTGTCGATGGTACACTC+GGG 0.682196 2.3:-71555925 MS.gene01773:CDS
CAATACCCAAAATCTTCCAG+AGG 0.693841 2.3:+71555949 None:intergenic
TGTAGCTCTAGCGCTCGTAG+AGG 0.710634 2.3:-71555879 MS.gene01773:CDS
ATGTGAACAAGATGTTTGTG+TGG 0.712148 2.3:-71555667 MS.gene01773:CDS
GGAGTGGTAACAGATGCTGG+AGG 0.815190 2.3:-71554527 MS.gene01773:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTAATATTCATGAATA+AGG - chr2.3:71556625-71556644 MS.gene01773:intron 10.0%
!!! ATAATATAGTTGTTTTTTTA+TGG - chr2.3:71557085-71557104 MS.gene01773:intron 10.0%
!! AAATATTAATGAAAAAGTTG+AGG - chr2.3:71555252-71555271 MS.gene01773:intron 15.0%
!! AGTTTACTAAAAATAATTAC+AGG - chr2.3:71556462-71556481 MS.gene01773:intron 15.0%
!! GTTTACTAAAAATAATTACA+GGG - chr2.3:71556463-71556482 MS.gene01773:intron 15.0%
!! TTAATATCTGTAAAAAAGAT+TGG - chr2.3:71556775-71556794 MS.gene01773:intron 15.0%
!!! TCTTTTTTACAGATATTAAT+AGG + chr2.3:71556774-71556793 None:intergenic 15.0%
!! AAGTTAAAGTAACTTATAAG+TGG + chr2.3:71555450-71555469 None:intergenic 20.0%
!! ATAGCTTAGAAATATAACAT+AGG + chr2.3:71555506-71555525 None:intergenic 20.0%
!! CAAAAGAATGATTATAAATG+AGG - chr2.3:71555061-71555080 MS.gene01773:intron 20.0%
!! TATAAACCTATAATATGCTT+AGG + chr2.3:71557068-71557087 None:intergenic 20.0%
!! TCAAAAGGATTATATTTAGA+AGG - chr2.3:71557304-71557323 MS.gene01773:CDS 20.0%
!! TCTAGAAATATTAGTTGTTA+CGG - chr2.3:71554748-71554767 MS.gene01773:intron 20.0%
!!! AATTAAGTTGCTTATCTAAA+CGG - chr2.3:71557040-71557059 MS.gene01773:intron 20.0%
!!! ACATGATATTCTTATTTACA+CGG - chr2.3:71556502-71556521 MS.gene01773:intron 20.0%
!!! TATGTTATATTTCTAAGCTA+TGG - chr2.3:71555505-71555524 MS.gene01773:intron 20.0%
!!! TATGTTTCCATTTTTATATG+CGG - chr2.3:71554959-71554978 MS.gene01773:intron 20.0%
! AAAATTTGTATTGCATGGTT+TGG + chr2.3:71556151-71556170 None:intergenic 25.0%
! AAGAATGATTATAAATGAGG+AGG - chr2.3:71555064-71555083 MS.gene01773:intron 25.0%
! AATTTGACCGCATATAAAAA+TGG + chr2.3:71554969-71554988 None:intergenic 25.0%
! ACTAGTTAAACCATACATTT+AGG + chr2.3:71556745-71556764 None:intergenic 25.0%
! AGACAAAAATTTGTATTGCA+TGG + chr2.3:71556156-71556175 None:intergenic 25.0%
! ATAAGCATTTATGAGTGTTT+CGG - chr2.3:71556885-71556904 MS.gene01773:intron 25.0%
! CTAGTGTAAACTAAAATCAT+TGG - chr2.3:71554993-71555012 MS.gene01773:intron 25.0%
! GAAGAGGTTGTTAAATAAAA+CGG - chr2.3:71556682-71556701 MS.gene01773:intron 25.0%
! GAATAAGATGCAATCATAAT+TGG - chr2.3:71555353-71555372 MS.gene01773:intron 25.0%
! GATTGGAAAATTGAAATCAT+TGG - chr2.3:71556792-71556811 MS.gene01773:intron 25.0%
! TATCTTGTAGCAATTTATCT+GGG + chr2.3:71556598-71556617 None:intergenic 25.0%
! TGAAAATGTAAAATGAACAC+TGG + chr2.3:71555304-71555323 None:intergenic 25.0%
! TTTAGGCTATTATGTCTAAT+AGG + chr2.3:71555098-71555117 None:intergenic 25.0%
!! ATCTTCTTTTTCTGATGATT+TGG + chr2.3:71554434-71554453 None:intergenic 25.0%
!!! TCCACTAAACGATTTTTTAA+AGG + chr2.3:71554911-71554930 None:intergenic 25.0%
!!! TTCTTATTTTCACAGCAAAA+AGG + chr2.3:71555029-71555048 None:intergenic 25.0%
AATGATCTTTCCATGTAATG+AGG + chr2.3:71557362-71557381 None:intergenic 30.0%
AATGGTTAAGTTATCAACAC+CGG - chr2.3:71555990-71556009 MS.gene01773:CDS 30.0%
AGATGATGAAGAATATTCCA+AGG - chr2.3:71554450-71554469 MS.gene01773:CDS 30.0%
AGGTATTCATGAAATTTGCA+AGG - chr2.3:71557179-71557198 MS.gene01773:intron 30.0%
AGGTTACAAGGAGAAAATAT+GGG - chr2.3:71557204-71557223 MS.gene01773:intron 30.0%
AGTAAAAGAATGGCTTGAAT+TGG + chr2.3:71556230-71556249 None:intergenic 30.0%
ATATTCCACACCTAAATGTA+TGG - chr2.3:71556732-71556751 MS.gene01773:intron 30.0%
ATCAAACAACAAAGATCAAG+AGG + chr2.3:71556343-71556362 None:intergenic 30.0%
ATGGTAAAATATGCAGCAAT+AGG - chr2.3:71557142-71557161 MS.gene01773:intron 30.0%
ATGTAAAATGAACACTGGAA+AGG + chr2.3:71555299-71555318 None:intergenic 30.0%
CTAAAAATTTACCTTGCAGT+AGG - chr2.3:71555113-71555132 MS.gene01773:intron 30.0%
CTATCTTGTAGCAATTTATC+TGG + chr2.3:71556599-71556618 None:intergenic 30.0%
GGTATTCATGAAATTTGCAA+GGG - chr2.3:71557180-71557199 MS.gene01773:intron 30.0%
TACAATGAGAAAGGAGATTA+TGG + chr2.3:71555411-71555430 None:intergenic 30.0%
TAGTTCAGATGCATTAGATT+TGG + chr2.3:71555690-71555709 None:intergenic 30.0%
TATAGCTGTTCTTAGGATTT+CGG - chr2.3:71555182-71555201 MS.gene01773:intron 30.0%
TGAAGAATATTCCAAGGAAT+TGG - chr2.3:71554456-71554475 MS.gene01773:CDS 30.0%
TGCAAATTTCATGAATACCT+CGG + chr2.3:71557179-71557198 None:intergenic 30.0%
TGTGTGCTATATGCTAAAAA+GGG - chr2.3:71556070-71556089 MS.gene01773:CDS 30.0%
TTAAACCATACATTTAGGTG+TGG + chr2.3:71556740-71556759 None:intergenic 30.0%
TTATTCATACATATCCTCAG+CGG + chr2.3:71556846-71556865 None:intergenic 30.0%
TTCGAACTTTACAATGAGAA+AGG + chr2.3:71555420-71555439 None:intergenic 30.0%
TTGTGTGCTATATGCTAAAA+AGG - chr2.3:71556069-71556088 MS.gene01773:CDS 30.0%
! ATTCCAACGGATAATTTTTC+CGG - chr2.3:71554782-71554801 MS.gene01773:intron 30.0%
! CTACTGCAAGGTAAATTTTT+AGG + chr2.3:71555115-71555134 None:intergenic 30.0%
! GCCTTTAAAAAATCGTTTAG+TGG - chr2.3:71554907-71554926 MS.gene01773:intron 30.0%
! TCTTTTCTTTTTCCTCAACT+GGG - chr2.3:71554607-71554626 MS.gene01773:CDS 30.0%
!! ATAATCCTTTTGAGAAGTCT+AGG + chr2.3:71557297-71557316 None:intergenic 30.0%
!! ATCATTGACAGTTTTAACCA+CGG + chr2.3:71555720-71555739 None:intergenic 30.0%
!! ATTGTGCTGTTTGAATAGAT+TGG - chr2.3:71555573-71555592 MS.gene01773:CDS 30.0%
!! TAATCCTTTTGAGAAGTCTA+GGG + chr2.3:71557296-71557315 None:intergenic 30.0%
!!! TTTTTAAAGGCATCTTTGAC+TGG + chr2.3:71554898-71554917 None:intergenic 30.0%
AAATAAGCCATGATATGTCC+AGG - chr2.3:71556919-71556938 MS.gene01773:intron 35.0%
AACAGTCGACATATTAGCAT+TGG - chr2.3:71556540-71556559 MS.gene01773:intron 35.0%
AATACCCAAAATCTTCCAGA+GGG + chr2.3:71555845-71555864 None:intergenic 35.0%
ACAAAGATCAAGAGGTGAAA+AGG + chr2.3:71556335-71556354 None:intergenic 35.0%
ACCATGCTGTATACTCTTAA+TGG + chr2.3:71557127-71557146 None:intergenic 35.0%
ATCTTGTTCACATGTAGCAA+TGG + chr2.3:71556118-71556137 None:intergenic 35.0%
ATGTGAACAAGATGTTTGTG+TGG - chr2.3:71556125-71556144 MS.gene01773:CDS 35.0%
CAAAGATCAAGAGGTGAAAA+GGG + chr2.3:71556334-71556353 None:intergenic 35.0%
CAGGTTACAAGGAGAAAATA+TGG - chr2.3:71557203-71557222 MS.gene01773:intron 35.0%
GATTTCCAAAACCAATCATC+AGG - chr2.3:71554540-71554559 MS.gene01773:CDS 35.0%
GCAACTTAATTAAGCGCTTA+AGG + chr2.3:71557031-71557050 None:intergenic 35.0%
GTTATCATCATACAAGAAGC+TGG - chr2.3:71557241-71557260 MS.gene01773:CDS 35.0%
GTTGAAAGAGAGAAAACAAG+AGG - chr2.3:71556370-71556389 MS.gene01773:intron 35.0%
GTTTATTTGGATGTGTGCAT+TGG - chr2.3:71554694-71554713 MS.gene01773:intron 35.0%
TATTTAGAAGGCTTAGATCG+CGG - chr2.3:71557316-71557335 MS.gene01773:CDS 35.0%
TCGATACAATCCTATGATAC+CGG + chr2.3:71556012-71556031 None:intergenic 35.0%
TCTAAGCTATGGTTGTATGA+TGG - chr2.3:71555516-71555535 MS.gene01773:intron 35.0%
TCTCATGTATAGCTGTTCTT+AGG - chr2.3:71555175-71555194 MS.gene01773:intron 35.0%
TGAGTGTTTCGGATATCTTA+TGG - chr2.3:71556896-71556915 MS.gene01773:intron 35.0%
TGATTTCATTGTTGCCACAT+GGG - chr2.3:71554672-71554691 MS.gene01773:CDS 35.0%
TGGATATTATCTGAGTGTCT+AGG - chr2.3:71555617-71555636 MS.gene01773:CDS 35.0%
TTGTTCCAACTTTCAGAAGT+TGG + chr2.3:71556046-71556065 None:intergenic 35.0%
! AATCAAGCAAGGTGTTTACT+AGG + chr2.3:71555152-71555171 None:intergenic 35.0%
! ACCATTAAGAGTATACAGCA+TGG - chr2.3:71557123-71557142 MS.gene01773:intron 35.0%
! ACTTCTGAAAGTTGGAACAA+AGG - chr2.3:71556046-71556065 MS.gene01773:CDS 35.0%
! CTATATGCTAAAAAGGGTAC+TGG - chr2.3:71556076-71556095 MS.gene01773:CDS 35.0%
! GAATTTCCAAGGTCTGATTT+TGG + chr2.3:71555779-71555798 None:intergenic 35.0%
! GTCTTTTCTTTTTCCTCAAC+TGG - chr2.3:71554606-71554625 MS.gene01773:CDS 35.0%
! TGTTTTCACATAAGCTATCC+TGG + chr2.3:71556940-71556959 None:intergenic 35.0%
! TTAGTTGTTACGGCAGTTTT+CGG - chr2.3:71554758-71554777 MS.gene01773:intron 35.0%
! TTTTTGTGAACCAGTTGAGA+AGG - chr2.3:71556204-71556223 MS.gene01773:CDS 35.0%
!! AATCCTTTTGAGAAGTCTAG+GGG + chr2.3:71557295-71557314 None:intergenic 35.0%
!!! TGTTTTTTTATGGTGTGTGC+AGG - chr2.3:71557095-71557114 MS.gene01773:intron 35.0%
AAAATATGGGATCATGCTGC+AGG - chr2.3:71557217-71557236 MS.gene01773:CDS 40.0%
AAATATGCAGCAATAGGTCG+TGG - chr2.3:71557148-71557167 MS.gene01773:intron 40.0%
AAGCTATCCTGGACATATCA+TGG + chr2.3:71556929-71556948 None:intergenic 40.0%
AATTCTCCTGTTACTGTTGC+TGG - chr2.3:71554580-71554599 MS.gene01773:CDS 40.0%
ACAACATAAATTTGCCGCTG+AGG - chr2.3:71556829-71556848 MS.gene01773:intron 40.0%
ACACCGGAAAAATTATCCGT+TGG + chr2.3:71554788-71554807 None:intergenic 40.0%
ACATGAGATGCAATCAAGCA+AGG + chr2.3:71555163-71555182 None:intergenic 40.0%
ACGGAGCCTAAGCATATTAT+AGG - chr2.3:71557059-71557078 MS.gene01773:intron 40.0%
ATCATCATACAAGAAGCTGG+TGG - chr2.3:71557244-71557263 MS.gene01773:CDS 40.0%
ATCTGAACTATTAGACTCCG+TGG - chr2.3:71555700-71555719 MS.gene01773:CDS 40.0%
CAAGTCCTGCAGTAAAAGAA+TGG + chr2.3:71556240-71556259 None:intergenic 40.0%
CAATACCCAAAATCTTCCAG+AGG + chr2.3:71555846-71555865 None:intergenic 40.0%
CAATCATCAGGTTCAGTCTA+AGG - chr2.3:71554552-71554571 MS.gene01773:CDS 40.0%
CACATCCAAATAAACCCATG+TGG + chr2.3:71554689-71554708 None:intergenic 40.0%
CGAGAACTTCTGAATTTCCA+AGG + chr2.3:71555790-71555809 None:intergenic 40.0%
CGTTGTGTAGTTGAAACACA+TGG + chr2.3:71556407-71556426 None:intergenic 40.0%
CTGATTTCATTGTTGCCACA+TGG - chr2.3:71554671-71554690 MS.gene01773:CDS 40.0%
CTTAGACTGAACCTGATGAT+TGG + chr2.3:71554554-71554573 None:intergenic 40.0%
GAAATGAAATGACCCAGTTG+AGG + chr2.3:71554622-71554641 None:intergenic 40.0%
GCGGCAAATTTATGTTGTCT+TGG + chr2.3:71556827-71556846 None:intergenic 40.0%
GTTATCAACACCGGTATCAT+AGG - chr2.3:71555999-71556018 MS.gene01773:CDS 40.0%
TGTGGCAACAATGAAATCAG+AGG + chr2.3:71554671-71554690 None:intergenic 40.0%
TGTTGCCACATGGGTTTATT+TGG - chr2.3:71554681-71554700 MS.gene01773:intron 40.0%
TTAACCATTCCTTCCGCATA+GGG + chr2.3:71555979-71555998 None:intergenic 40.0%
TTAGCACTCTTGTGATGAAG+AGG - chr2.3:71556666-71556685 MS.gene01773:intron 40.0%
TTCATGAAATTTGCAAGGGC+AGG - chr2.3:71557184-71557203 MS.gene01773:intron 40.0%
TTCCAACTTTCAGAAGTTGG+AGG + chr2.3:71556043-71556062 None:intergenic 40.0%
TTGGATGTGTGCATTGGAAT+TGG - chr2.3:71554700-71554719 MS.gene01773:intron 40.0%
! ACAAAGACTCCACTAGTTTG+TGG + chr2.3:71554938-71554957 None:intergenic 40.0%
! CTCAACTGGTTCACAAAAAG+TGG + chr2.3:71556203-71556222 None:intergenic 40.0%
! GAGAGAAAACAAGAGGCATA+TGG - chr2.3:71556377-71556396 MS.gene01773:intron 40.0%
! GAGGATATTTTCCTACTGCA+AGG + chr2.3:71555127-71555146 None:intergenic 40.0%
! TCAAGCCATTCTTTTACTGC+AGG - chr2.3:71556232-71556251 MS.gene01773:intron 40.0%
!! CACAAGAGTGCTAATTCAAC+AGG + chr2.3:71556660-71556679 None:intergenic 40.0%
!! CTCTGGAAGATTTTGGGTAT+TGG - chr2.3:71555844-71555863 MS.gene01773:CDS 40.0%
!! TAGCATTGACAATCCAGCTT+TGG - chr2.3:71556177-71556196 MS.gene01773:CDS 40.0%
!! TGCTAAAAAGGGTACTGGAA+AGG - chr2.3:71556081-71556100 MS.gene01773:CDS 40.0%
!!! CTGAACCTGATGATTGGTTT+TGG + chr2.3:71554548-71554567 None:intergenic 40.0%
AACGGCAACATCCAATTCCT+TGG + chr2.3:71554470-71554489 None:intergenic 45.0%
AGCCATTTGCACTGCTCTAA+CGG + chr2.3:71554488-71554507 None:intergenic 45.0%
ATAAGTCGCTGCAACTCAAC+TGG - chr2.3:71555815-71555834 MS.gene01773:CDS 45.0%
ATATCCAGCTGACAACTGCT+TGG + chr2.3:71555604-71555623 None:intergenic 45.0%
ATTACCCTATGCGGAAGGAA+TGG - chr2.3:71555972-71555991 MS.gene01773:CDS 45.0%
ATTTGCAAGGGCAGGTTACA+AGG - chr2.3:71557192-71557211 MS.gene01773:intron 45.0%
CAGAAGCCAAAATCAGACCT+TGG - chr2.3:71555770-71555789 MS.gene01773:CDS 45.0%
CGCGGTATAATTGCTTGTTC+TGG - chr2.3:71557334-71557353 MS.gene01773:CDS 45.0%
CTTAACCATTCCTTCCGCAT+AGG + chr2.3:71555980-71555999 None:intergenic 45.0%
GACTAACCAGCAACAGTAAC+AGG + chr2.3:71554589-71554608 None:intergenic 45.0%
GCTGTCCAAATTACCCTATG+CGG - chr2.3:71555963-71555982 MS.gene01773:CDS 45.0%
TCACAAAAAGTGGCCAAAGC+TGG + chr2.3:71556193-71556212 None:intergenic 45.0%
TCCAAATTACCCTATGCGGA+AGG - chr2.3:71555967-71555986 MS.gene01773:CDS 45.0%
TCCTTCCGCATAGGGTAATT+TGG + chr2.3:71555971-71555990 None:intergenic 45.0%
TGATGCTGTTGATGCTAGCT+GGG - chr2.3:71557381-71557400 MS.gene01773:CDS 45.0%
TGTCCCCTAGACTTCTCAAA+AGG - chr2.3:71557289-71557308 MS.gene01773:CDS 45.0%
TTGATGCTGTTGATGCTAGC+TGG - chr2.3:71557380-71557399 MS.gene01773:CDS 45.0%
! CTCCTCCAACTTCTGAAAGT+TGG - chr2.3:71556038-71556057 MS.gene01773:CDS 45.0%
! GACGCAGCTTTTTGTAGTAG+AGG - chr2.3:71554840-71554859 MS.gene01773:intron 45.0%
! GCAAGGTGTTTACTAGGAAG+AGG + chr2.3:71555146-71555165 None:intergenic 45.0%
! GGCAGTTTTCGGTATTCCAA+CGG - chr2.3:71554769-71554788 MS.gene01773:intron 45.0%
! TTCACTTTGTCAGAGAGTGC+AGG - chr2.3:71554510-71554529 MS.gene01773:CDS 45.0%
!! AAAAGGGTACTGGAAAGGCT+TGG - chr2.3:71556086-71556105 MS.gene01773:CDS 45.0%
!! CTTGAATTGGCCTTCTCAAC+TGG + chr2.3:71556217-71556236 None:intergenic 45.0%
!! TGGTCCCTCTGGAAGATTTT+GGG - chr2.3:71555838-71555857 MS.gene01773:CDS 45.0%
!! TGTTCTGGAGCCTCATTACA+TGG - chr2.3:71557349-71557368 MS.gene01773:CDS 45.0%
ACCAACCGAGCTGCTTACTT+GGG - chr2.3:71554818-71554837 MS.gene01773:intron 50.0%
ATCCTGTCGATGGTACACTC+GGG - chr2.3:71555867-71555886 MS.gene01773:CDS 50.0%
CATACAAGAAGCTGGTGGAG+TGG - chr2.3:71557249-71557268 MS.gene01773:CDS 50.0%
GATCCTGTCGATGGTACACT+CGG - chr2.3:71555866-71555885 MS.gene01773:CDS 50.0%
GATGCTGTTGATGCTAGCTG+GGG - chr2.3:71557382-71557401 MS.gene01773:CDS 50.0%
GGCTTCTGAACACCAAATCG+AGG + chr2.3:71555758-71555777 None:intergenic 50.0%
GTGGAGACACCACAAACTAG+TGG - chr2.3:71554926-71554945 MS.gene01773:intron 50.0%
TACCAACCGAGCTGCTTACT+TGG - chr2.3:71554817-71554836 MS.gene01773:intron 50.0%
TGCCGTTAGAGCAGTGCAAA+TGG - chr2.3:71554483-71554502 MS.gene01773:CDS 50.0%
! GTTGGTAAGCTAAGGGACAC+CGG + chr2.3:71554804-71554823 None:intergenic 50.0%
!! CAGCTCGGTTGGTAAGCTAA+GGG + chr2.3:71554811-71554830 None:intergenic 50.0%
!! GAAGCAGTGCTTGGTGTTCT+TGG - chr2.3:71555941-71555960 MS.gene01773:CDS 50.0%
!! GTGGTCCCTCTGGAAGATTT+TGG - chr2.3:71555837-71555856 MS.gene01773:CDS 50.0%
!! TGGGTATTGGATCCTGTCGA+TGG - chr2.3:71555857-71555876 MS.gene01773:CDS 50.0%
!! TTGCTCACAGTGTTGGTCTC+AGG - chr2.3:71556257-71556276 MS.gene01773:intron 50.0%
AACCCGAGTGTACCATCGAC+AGG + chr2.3:71555872-71555891 None:intergenic 55.0%
AACTCAACTGGTGGTCCCTC+TGG - chr2.3:71555827-71555846 MS.gene01773:CDS 55.0%
AATAGGTCGTGGAGATGCCG+AGG - chr2.3:71557159-71557178 MS.gene01773:intron 55.0%
AGTCGCTGCAACTCAACTGG+TGG - chr2.3:71555818-71555837 MS.gene01773:CDS 55.0%
GAGGATGGAGAAGCAGTGCT+TGG - chr2.3:71555932-71555951 MS.gene01773:CDS 55.0%
GGAGTGGTAACAGATGCTGG+AGG - chr2.3:71557265-71557284 MS.gene01773:CDS 55.0%
GGTGGAGTGGTAACAGATGC+TGG - chr2.3:71557262-71557281 MS.gene01773:CDS 55.0%
GTGTCCAAGCAGTTGTCAGC+TGG - chr2.3:71555597-71555616 MS.gene01773:CDS 55.0%
TCCCAAGTAAGCAGCTCGGT+TGG + chr2.3:71554822-71554841 None:intergenic 55.0%
TGCGTCCCAAGTAAGCAGCT+CGG + chr2.3:71554826-71554845 None:intergenic 55.0%
! CAAATCGAGGCGCTTCAGCT+AGG + chr2.3:71555745-71555764 None:intergenic 55.0%
! CTAGCTGAAGCGCCTCGATT+TGG - chr2.3:71555743-71555762 MS.gene01773:CDS 55.0%
! GCAGGACTTGCTCACAGTGT+TGG - chr2.3:71556250-71556269 MS.gene01773:intron 55.0%
! GGTACACTCGGGTTTGTTCG+TGG - chr2.3:71555878-71555897 MS.gene01773:CDS 55.0%
!! GCAGCTCGGTTGGTAAGCTA+AGG + chr2.3:71554812-71554831 None:intergenic 55.0%
!! TGTAGCTCTAGCGCTCGTAG+AGG - chr2.3:71555913-71555932 MS.gene01773:CDS 55.0%
GCTCTAGCGCTCGTAGAGGA+TGG - chr2.3:71555917-71555936 MS.gene01773:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 71554394 71557420 71554394 ID=MS.gene01773
chr2.3 mRNA 71554394 71557420 71554394 ID=MS.gene01773.t1;Parent=MS.gene01773
chr2.3 exon 71557213 71557420 71557213 ID=MS.gene01773.t1.exon1;Parent=MS.gene01773.t1
chr2.3 CDS 71557213 71557420 71557213 ID=cds.MS.gene01773.t1;Parent=MS.gene01773.t1
chr2.3 exon 71555536 71556223 71555536 ID=MS.gene01773.t1.exon2;Parent=MS.gene01773.t1
chr2.3 CDS 71555536 71556223 71555536 ID=cds.MS.gene01773.t1;Parent=MS.gene01773.t1
chr2.3 exon 71554394 71554697 71554394 ID=MS.gene01773.t1.exon3;Parent=MS.gene01773.t1
chr2.3 CDS 71554394 71554697 71554394 ID=cds.MS.gene01773.t1;Parent=MS.gene01773.t1
Gene Sequence

>MS.gene01773

ATGGAGGATGAGGATCAGAAAAAGTTGAGTTTTTTCTCCAAATCATCAGAAAAAGAAGATGATGAAGAATATTCCAAGGAATTGGATGTTGCCGTTAGAGCAGTGCAAATGGCTTGTTCACTTTGTCAGAGAGTGCAGGAGAGTTTGATTTCCAAAACCAATCATCAGGTTCAGTCTAAGGATGATAATTCTCCTGTTACTGTTGCTGGTTAGTCTTTTCTTTTTCCTCAACTGGGTCATTTCATTTCATTTCATATACAAGTTCTTTATTTTTCCTCTGATTTCATTGTTGCCACATGGGTTTATTTGGATGTGTGCATTGGAATTGGATATTATATTGTCTTGCATCAAATTTCTAGAAATATTAGTTGTTACGGCAGTTTTCGGTATTCCAACGGATAATTTTTCCGGTGTCCCTTAGCTTACCAACCGAGCTGCTTACTTGGGACGCAGCTTTTTGTAGTAGAGGTTTATTTAGTGAACTTAGCAATTATACAAACTCCAGTCAAAGATGCCTTTAAAAAATCGTTTAGTGGAGACACCACAAACTAGTGGAGTCTTTGTCTATGTTTCCATTTTTATATGCGGTCAAATTATGACTAGTGTAAACTAAAATCATTGGTGTGAAATATCCTTTTTGCTGTGAAAATAAGAAATTCTATTGAAACAAAAGAATGATTATAAATGAGGAGGATAAGTATCCTATTAGACATAATAGCCTAAAAATTTACCTTGCAGTAGGAAAATATCCTCTTCCTAGTAAACACCTTGCTTGATTGCATCTCATGTATAGCTGTTCTTAGGATTTCGGAAGCACATTTATGCTTTTGAAAGAATACAAGTTTTCAAAAGATTGAAAAATATTAATGAAAAAGTTGAGGTGATTTTTCAATTTATTTAGACCTTTCCAGTGTTCATTTTACATTTTCAACTTTACATGTTGCTATTAGCATTGAATTGAATAAGATGCAATCATAATTGGAATCATGATCTTCATAATTAATTTATTACATACCATAATCTCCTTTCTCATTGTAAAGTTCGAATGTATGACCACTTATAAGTTACTTTAACTTGAGCAAAGATCTATGATTAAATTTTTGCTGTGTCCTATGTTATATTTCTAAGCTATGGTTGTATGATGGTTGAAGCTTTTCTGAGTTTTGTACATAAAATTTCATTGTGCTGTTTGAATAGATTGGAGTGTCCAAGCAGTTGTCAGCTGGATATTATCTGAGTGTCTAGGAAGCGAAAATATCTCAATTGTAGCTGAAGAAGATGTTCAAACTCTCTCCAAATCTAATGCATCTGAACTATTAGACTCCGTGGTTAAAACTGTCAATGATTGCCTAGCTGAAGCGCCTCGATTTGGTGTTCAGAAGCCAAAATCAGACCTTGGAAATTCAGAAGTTCTCGATATTATAAGTCGCTGCAACTCAACTGGTGGTCCCTCTGGAAGATTTTGGGTATTGGATCCTGTCGATGGTACACTCGGGTTTGTTCGTGGCGATCAATATGCTGTAGCTCTAGCGCTCGTAGAGGATGGAGAAGCAGTGCTTGGTGTTCTTGGCTGTCCAAATTACCCTATGCGGAAGGAATGGTTAAGTTATCAACACCGGTATCATAGGATTGTATCGAAGTTGACTCCTCCAACTTCTGAAAGTTGGAACAAAGGTTGTGTGCTATATGCTAAAAAGGGTACTGGAAAGGCTTGGATGCAACCATTGCTACATGTGAACAAGATGTTTGTGTGGCCAAACCATGCAATACAAATTTTTGTCTCTAGCATTGACAATCCAGCTTTGGCCACTTTTTGTGAACCAGTTGAGAAGGCCAATTCAAGCCATTCTTTTACTGCAGGACTTGCTCACAGTGTTGGTCTCAGGTACATTTATCTTTTTTCAATTTCATTTCGATATCAATTACTCTCTGCGTCTCCCTTTTCACCTCTTGATCTTTGTTGTTTGATCATTGATGTTGAAAGAGAGAAAACAAGAGGCATATGGTGAACCATGTGTTTCAACTACACAACGTAGATTCTTCATATTGACTAAAATTTGTTTGTGAAAGTTTACTAAAAATAATTACAGGGTATTCTACTCGTAGTTACATGATATTCTTATTTACACGGTCATATTTTATCTTCAACAGTCGACATATTAGCATTGGATTATTATGATGTTCTTGTTTTTTTTAAATATCCCAGATAAATTGCTACAAGATAGATTCTAAAATTTAATATTCATGAATAAGGTCGAATAATCCTGTTGAATTAGCACTCTTGTGATGAAGAGGTTGTTAAATAAAACGGAAAGCAGAAATTGTGATGTGTAGTTTTATATTCCACACCTAAATGTATGGTTTAACTAGTTAGATTCCTATTAATATCTGTAAAAAAGATTGGAAAATTGAAATCATTGGAAAAGATAACCAAGACAACATAAATTTGCCGCTGAGGATATGTATGAATAACTTGTGTTTATTTACTAGAATAAGCATTTATGAGTGTTTCGGATATCTTATGGAAATAAGCCATGATATGTCCAGGATAGCTTATGTGAAAACAGTTTGACTTTATTTTATATTGTTATAGAAATAGATTATACATAAACTCTTAAACGATAAGCACTTATGCCTTAAGCGCTTAATTAAGTTGCTTATCTAAACGGAGCCTAAGCATATTATAGGTTTATAATATAGTTGTTTTTTTATGGTGTGTGCAGGAAACAACCATTAAGAGTATACAGCATGGTAAAATATGCAGCAATAGGTCGTGGAGATGCCGAGGTATTCATGAAATTTGCAAGGGCAGGTTACAAGGAGAAAATATGGGATCATGCTGCAGGTGTTATCATCATACAAGAAGCTGGTGGAGTGGTAACAGATGCTGGAGGATGTCCCCTAGACTTCTCAAAAGGATTATATTTAGAAGGCTTAGATCGCGGTATAATTGCTTGTTCTGGAGCCTCATTACATGGAAAGATCATTGATGCTGTTGATGCTAGCTGGGGCTGTTCTAGCCTTTGA
Protein sequence

>MS.gene01773.t1

MEDEDQKKLSFFSKSSEKEDDEEYSKELDVAVRAVQMACSLCQRVQESLISKTNHQVQSKDDNSPVTVADWSVQAVVSWILSECLGSENISIVAEEDVQTLSKSNASELLDSVVKTVNDCLAEAPRFGVQKPKSDLGNSEVLDIISRCNSTGGPSGRFWVLDPVDGTLGFVRGDQYAVALALVEDGEAVLGVLGCPNYPMRKEWLSYQHRYHRIVSKLTPPTSESWNKGCVLYAKKGTGKAWMQPLLHVNKMFVWPNHAIQIFVSSIDNPALATFCEPVEKANSSHSFTAGLAHSVGLRKQPLRVYSMVKYAAIGRGDAEVFMKFARAGYKEKIWDHAAGVIIIQEAGGVVTDAGGCPLDFSKGLYLEGLDRGIIACSGASLHGKIIDAVDASWGCSSL