Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018837.t1 | XP_003628625.1 | 97.3 | 73 | 2 | 0 | 1 | 73 | 1 | 73 | 1.20E-32 | 149.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018837.t1 | P58728 | 89.0 | 73 | 8 | 0 | 1 | 73 | 1 | 73 | 8.0e-33 | 141.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018837.t1 | G7IW51 | 97.3 | 73 | 2 | 0 | 1 | 73 | 1 | 73 | 8.8e-33 | 149.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene65850 | MS.gene018837 | PPI |
| MS.gene018837 | MS.gene85479 | PPI |
| MS.gene85479 | MS.gene018837 | PPI |
| MS.gene018837 | MS.gene039781 | PPI |
| MS.gene018837 | MS.gene040992 | PPI |
| MS.gene78633 | MS.gene018837 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018837.t1 | MTR_3g019310 | 97.260 | 73 | 2 | 0 | 1 | 73 | 1 | 73 | 2.68e-48 | 150 |
| MS.gene018837.t1 | MTR_3g019300 | 97.260 | 73 | 2 | 0 | 1 | 73 | 1 | 73 | 2.68e-48 | 150 |
| MS.gene018837.t1 | MTR_8g062750 | 97.260 | 73 | 2 | 0 | 1 | 73 | 1 | 73 | 2.93e-48 | 150 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018837.t1 | AT4G14342 | 70.297 | 101 | 28 | 1 | 1 | 101 | 1 | 99 | 3.49e-45 | 143 |
| MS.gene018837.t1 | AT4G14342 | 89.041 | 73 | 8 | 0 | 1 | 73 | 1 | 73 | 5.55e-45 | 142 |
| MS.gene018837.t1 | AT3G23325 | 87.671 | 73 | 9 | 0 | 1 | 73 | 1 | 73 | 2.66e-44 | 140 |
Find 34 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAACTCTGGTCGGTTATT+TGG | 0.170901 | 1.2:-37835029 | MS.gene018837:CDS |
| CCAAATAACCGACCAGAGTT+TGG | 0.252627 | 1.2:+37835029 | None:intergenic |
| GATAGCTATGCTTCGTATAT+TGG | 0.282004 | 1.2:-37835435 | MS.gene018837:CDS |
| TGACAGAGAATTGGGGCCTT+TGG | 0.304093 | 1.2:-37834927 | MS.gene018837:CDS |
| CAAGCGATTTGACAGAGAAT+TGG | 0.308184 | 1.2:-37834936 | MS.gene018837:CDS |
| AAGCGATTTGACAGAGAATT+GGG | 0.345762 | 1.2:-37834935 | MS.gene018837:CDS |
| GACAGAGAATTGGGGCCTTT+GGG | 0.347567 | 1.2:-37834926 | MS.gene018837:CDS |
| ATTGCTCAAAACGAATCTAT+TGG | 0.369668 | 1.2:-37835384 | MS.gene018837:CDS |
| AGAATTGGGGCCTTTGGGTT+GGG | 0.369816 | 1.2:-37834921 | MS.gene018837:CDS |
| CAAATAACCGACCAGAGTTT+GGG | 0.405326 | 1.2:+37835030 | None:intergenic |
| TAGAAAAGTAAGAAAGTAAA+GGG | 0.419141 | 1.2:+37835406 | None:intergenic |
| GCAATATGCAGGCGAGTGAT+AGG | 0.420071 | 1.2:-37835557 | None:intergenic |
| CTAAAGCATTTGCCGCAAAT+AGG | 0.422416 | 1.2:-37835073 | MS.gene018837:CDS |
| TCTGGTCGGTTATTTGGAAA+TGG | 0.439376 | 1.2:-37835023 | MS.gene018837:CDS |
| CAAGCTAAATATGTTGGAAC+TGG | 0.450379 | 1.2:-37835504 | MS.gene018837:CDS |
| ATAGAAAAGTAAGAAAGTAA+AGG | 0.454525 | 1.2:+37835405 | None:intergenic |
| GAGAATTGGGGCCTTTGGGT+TGG | 0.461778 | 1.2:-37834922 | MS.gene018837:CDS |
| GACCGTGATTTAGAAATAAT+AGG | 0.464747 | 1.2:-37834980 | MS.gene018837:CDS |
| GTCGGTTATTTGGAAATGGC+AGG | 0.466962 | 1.2:-37835019 | MS.gene018837:CDS |
| CTGATATCAATAGATTTGAA+TGG | 0.473717 | 1.2:-37835476 | MS.gene018837:CDS |
| AGCTTGCAGATGCTCGAGTT+GGG | 0.481702 | 1.2:+37835521 | None:intergenic |
| AGTAAGAAAGTAAAGGGTAA+TGG | 0.502423 | 1.2:+37835412 | None:intergenic |
| TAGCTTGCAGATGCTCGAGT+TGG | 0.512932 | 1.2:+37835520 | None:intergenic |
| CATCTGCAAGCTAAATATGT+TGG | 0.518362 | 1.2:-37835510 | MS.gene018837:CDS |
| CTCTCCTCGACGCCTATTTG+CGG | 0.519190 | 1.2:+37835061 | None:intergenic |
| ATCCTATTATTTCTAAATCA+CGG | 0.522724 | 1.2:+37834978 | None:intergenic |
| AATAATAGGATACGATGAGA+AGG | 0.549854 | 1.2:-37834966 | MS.gene018837:CDS |
| TGATATCAATAGATTTGAAT+GGG | 0.559675 | 1.2:-37835475 | MS.gene018837:CDS |
| GAGTGTCGGTACCCAAACTC+TGG | 0.588931 | 1.2:-37835041 | MS.gene018837:CDS |
| GTCGGTACCCAAACTCTGGT+CGG | 0.618939 | 1.2:-37835037 | MS.gene018837:CDS |
| ATAGGCGTCGAGGAGAGTGT+CGG | 0.643134 | 1.2:-37835055 | MS.gene018837:CDS |
| AGCGATTTGACAGAGAATTG+GGG | 0.653308 | 1.2:-37834934 | MS.gene018837:CDS |
| GGATATGAATCCCAACCCAA+AGG | 0.674653 | 1.2:+37834911 | None:intergenic |
| TTTGCCGCAAATAGGCGTCG+AGG | 0.702272 | 1.2:-37835065 | MS.gene018837:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAAAATAGTAACGATAT+AGG | + | chr1.2:37835160-37835179 | None:intergenic | 20.0% |
| !! | ATAGAAAAGTAAGAAAGTAA+AGG | + | chr1.2:37835048-37835067 | None:intergenic | 20.0% |
| !! | TAGAAAAGTAAGAAAGTAAA+GGG | + | chr1.2:37835047-37835066 | None:intergenic | 20.0% |
| !! | TGATATCAATAGATTTGAAT+GGG | - | chr1.2:37834975-37834994 | MS.gene018837:CDS | 20.0% |
| !!! | ATCCTATTATTTCTAAATCA+CGG | + | chr1.2:37835475-37835494 | None:intergenic | 20.0% |
| ! | CTGATATCAATAGATTTGAA+TGG | - | chr1.2:37834974-37834993 | MS.gene018837:CDS | 25.0% |
| AATAATAGGATACGATGAGA+AGG | - | chr1.2:37835484-37835503 | MS.gene018837:CDS | 30.0% | |
| AGTAAGAAAGTAAAGGGTAA+TGG | + | chr1.2:37835041-37835060 | None:intergenic | 30.0% | |
| ATTGCTCAAAACGAATCTAT+TGG | - | chr1.2:37835066-37835085 | MS.gene018837:CDS | 30.0% | |
| GACCGTGATTTAGAAATAAT+AGG | - | chr1.2:37835470-37835489 | MS.gene018837:CDS | 30.0% | |
| AAGCGATTTGACAGAGAATT+GGG | - | chr1.2:37835515-37835534 | MS.gene018837:CDS | 35.0% | |
| CAAGCTAAATATGTTGGAAC+TGG | - | chr1.2:37834946-37834965 | MS.gene018837:CDS | 35.0% | |
| CATCTGCAAGCTAAATATGT+TGG | - | chr1.2:37834940-37834959 | MS.gene018837:CDS | 35.0% | |
| CGCTTTATGATGTCAAAACA+TGG | - | chr1.2:37835205-37835224 | MS.gene018837:intron | 35.0% | |
| GATAGCTATGCTTCGTATAT+TGG | - | chr1.2:37835015-37835034 | MS.gene018837:CDS | 35.0% | |
| TCAAAACATGGAACTTGGTT+AGG | - | chr1.2:37835217-37835236 | MS.gene018837:intron | 35.0% | |
| TGATGTCAAAACATGGAACT+TGG | - | chr1.2:37835212-37835231 | MS.gene018837:intron | 35.0% | |
| TTGACACATCAACTTGTAGT+AGG | - | chr1.2:37835255-37835274 | MS.gene018837:intron | 35.0% | |
| AGCGATTTGACAGAGAATTG+GGG | - | chr1.2:37835516-37835535 | MS.gene018837:CDS | 40.0% | |
| CAAATAACCGACCAGAGTTT+GGG | + | chr1.2:37835423-37835442 | None:intergenic | 40.0% | |
| CAAGCGATTTGACAGAGAAT+TGG | - | chr1.2:37835514-37835533 | MS.gene018837:CDS | 40.0% | |
| TATTTCGCTATTGCCTGCTA+TGG | + | chr1.2:37835328-37835347 | None:intergenic | 40.0% | |
| TCTGGTCGGTTATTTGGAAA+TGG | - | chr1.2:37835427-37835446 | MS.gene018837:CDS | 40.0% | |
| ! | ACTGCGTTACACTTTTATGC+AGG | - | chr1.2:37835092-37835111 | MS.gene018837:intron | 40.0% |
| ! | CTAAAGCATTTGCCGCAAAT+AGG | - | chr1.2:37835377-37835396 | MS.gene018837:CDS | 40.0% |
| AGCAGGCAATAGCGAAATAG+CGG | - | chr1.2:37835329-37835348 | MS.gene018837:intron | 45.0% | |
| CCAAACTCTGGTCGGTTATT+TGG | - | chr1.2:37835421-37835440 | MS.gene018837:CDS | 45.0% | |
| CCAAATAACCGACCAGAGTT+TGG | + | chr1.2:37835424-37835443 | None:intergenic | 45.0% | |
| GGATATGAATCCCAACCCAA+AGG | + | chr1.2:37835542-37835561 | None:intergenic | 45.0% | |
| GTCGGTTATTTGGAAATGGC+AGG | - | chr1.2:37835431-37835450 | MS.gene018837:CDS | 45.0% | |
| AGCTTGCAGATGCTCGAGTT+GGG | + | chr1.2:37834932-37834951 | None:intergenic | 50.0% | |
| GACAGAGAATTGGGGCCTTT+GGG | - | chr1.2:37835524-37835543 | MS.gene018837:CDS | 50.0% | |
| TGACAGAGAATTGGGGCCTT+TGG | - | chr1.2:37835523-37835542 | MS.gene018837:CDS | 50.0% | |
| ! | AGAATTGGGGCCTTTGGGTT+GGG | - | chr1.2:37835529-37835548 | MS.gene018837:CDS | 50.0% |
| ! | GCAATAGCGAAATAGCGGAG+AGG | - | chr1.2:37835334-37835353 | MS.gene018837:intron | 50.0% |
| ! | GCGAAATAGCGGAGAGGATT+AGG | - | chr1.2:37835340-37835359 | MS.gene018837:intron | 50.0% |
| ! | TAGCTTGCAGATGCTCGAGT+TGG | + | chr1.2:37834933-37834952 | None:intergenic | 50.0% |
| CTCTCCTCGACGCCTATTTG+CGG | + | chr1.2:37835392-37835411 | None:intergenic | 55.0% | |
| GAGAATTGGGGCCTTTGGGT+TGG | - | chr1.2:37835528-37835547 | MS.gene018837:CDS | 55.0% | |
| GTCGGTACCCAAACTCTGGT+CGG | - | chr1.2:37835413-37835432 | MS.gene018837:CDS | 55.0% | |
| ! | TTTGCCGCAAATAGGCGTCG+AGG | - | chr1.2:37835385-37835404 | MS.gene018837:CDS | 55.0% |
| !! | ATAGGCGTCGAGGAGAGTGT+CGG | - | chr1.2:37835395-37835414 | MS.gene018837:CDS | 55.0% |
| !! | GAGTGTCGGTACCCAAACTC+TGG | - | chr1.2:37835409-37835428 | MS.gene018837:CDS | 55.0% |
| CTGTGCTGTGCTGCCATAGC+AGG | - | chr1.2:37835312-37835331 | MS.gene018837:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 37834898 | 37835574 | 37834898 | ID=MS.gene018837 |
| chr1.2 | mRNA | 37834898 | 37835574 | 37834898 | ID=MS.gene018837.t1;Parent=MS.gene018837 |
| chr1.2 | exon | 37835359 | 37835574 | 37835359 | ID=MS.gene018837.t1.exon1;Parent=MS.gene018837.t1 |
| chr1.2 | CDS | 37835359 | 37835574 | 37835359 | ID=cds.MS.gene018837.t1;Parent=MS.gene018837.t1 |
| chr1.2 | exon | 37834898 | 37835110 | 37834898 | ID=MS.gene018837.t1.exon2;Parent=MS.gene018837.t1 |
| chr1.2 | CDS | 37834898 | 37835110 | 37834898 | ID=cds.MS.gene018837.t1;Parent=MS.gene018837.t1 |
| Gene Sequence |
| Protein sequence |
