Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78633.t1 | XP_003628625.1 | 98.6 | 73 | 1 | 0 | 1 | 73 | 1 | 73 | 2.50E-33 | 151.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78633.t1 | P58728 | 89.0 | 73 | 8 | 0 | 1 | 73 | 1 | 73 | 2.8e-33 | 142.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78633.t1 | G7IW51 | 98.6 | 73 | 1 | 0 | 1 | 73 | 1 | 73 | 1.8e-33 | 151.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene78633 | MS.gene65850 | PPI |
| MS.gene78633 | MS.gene040992 | PPI |
| MS.gene78633 | MS.gene039781 | PPI |
| MS.gene78633 | MS.gene85479 | PPI |
| MS.gene78633 | MS.gene018837 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78633.t1 | MTR_3g019310 | 87.952 | 83 | 10 | 0 | 1 | 83 | 1 | 83 | 1.57e-49 | 153 |
| MS.gene78633.t1 | MTR_3g019300 | 87.952 | 83 | 10 | 0 | 1 | 83 | 1 | 83 | 1.57e-49 | 153 |
| MS.gene78633.t1 | MTR_8g062750 | 98.630 | 73 | 1 | 0 | 1 | 73 | 1 | 73 | 2.33e-49 | 153 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78633.t1 | AT4G14342 | 70.297 | 101 | 28 | 1 | 1 | 101 | 1 | 99 | 3.62e-46 | 145 |
| MS.gene78633.t1 | AT4G14342 | 89.041 | 73 | 8 | 0 | 1 | 73 | 1 | 73 | 1.12e-45 | 144 |
| MS.gene78633.t1 | AT3G23325 | 87.671 | 73 | 9 | 0 | 1 | 73 | 1 | 73 | 5.93e-45 | 142 |
Find 34 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAACTCTGGTCGGTTATT+TGG | 0.170901 | 1.4:+42098439 | MS.gene78633:CDS |
| CCAAATAACCGACCAGAGTT+TGG | 0.252627 | 1.4:-42098439 | None:intergenic |
| GATAGCTATGCTTCGTATAT+TGG | 0.282004 | 1.4:+42098033 | MS.gene78633:CDS |
| TGACAGAGAATTGGGGCCTT+TGG | 0.304093 | 1.4:+42098541 | MS.gene78633:CDS |
| CAAGCGATTTGACAGAGAAT+TGG | 0.308184 | 1.4:+42098532 | MS.gene78633:CDS |
| AAGCGATTTGACAGAGAATT+GGG | 0.345762 | 1.4:+42098533 | MS.gene78633:CDS |
| GACAGAGAATTGGGGCCTTT+GGG | 0.347567 | 1.4:+42098542 | MS.gene78633:CDS |
| CTAAAGCGTTTGCCGCAAAT+AGG | 0.359925 | 1.4:+42098395 | MS.gene78633:CDS |
| ATTGCTCAAAACGAATCTAT+TGG | 0.369668 | 1.4:+42098084 | MS.gene78633:CDS |
| AGAATTGGGGCCTTTGGGTT+GGG | 0.369816 | 1.4:+42098547 | MS.gene78633:CDS |
| CAAATAACCGACCAGAGTTT+GGG | 0.405326 | 1.4:-42098438 | None:intergenic |
| TAGAAAAGTAAGAAAGTAAA+GGG | 0.419141 | 1.4:-42098062 | None:intergenic |
| GCAATATGCAGGCGAGTGAT+AGG | 0.420071 | 1.4:+42097911 | None:intergenic |
| TCTGGTCGGTTATTTGGAAA+TGG | 0.439376 | 1.4:+42098445 | MS.gene78633:CDS |
| CAAGCTAAATATGTTGGAAC+TGG | 0.450379 | 1.4:+42097964 | MS.gene78633:CDS |
| ATAGAAAAGTAAGAAAGTAA+AGG | 0.454525 | 1.4:-42098063 | None:intergenic |
| GAGAATTGGGGCCTTTGGGT+TGG | 0.461778 | 1.4:+42098546 | MS.gene78633:CDS |
| GACCGTGATTTAGAAATAAT+AGG | 0.464747 | 1.4:+42098488 | MS.gene78633:CDS |
| GTCGGTTATTTGGAAATGGC+AGG | 0.466962 | 1.4:+42098449 | MS.gene78633:CDS |
| CTGATATCAATAGATTTGAA+TGG | 0.473717 | 1.4:+42097992 | MS.gene78633:CDS |
| AGCTTGCAGATGCTCGAGTT+GGG | 0.481702 | 1.4:-42097947 | None:intergenic |
| AGTAAGAAAGTAAAGGGTAA+TGG | 0.502423 | 1.4:-42098056 | None:intergenic |
| TAGCTTGCAGATGCTCGAGT+TGG | 0.512932 | 1.4:-42097948 | None:intergenic |
| CATCTGCAAGCTAAATATGT+TGG | 0.518362 | 1.4:+42097958 | MS.gene78633:CDS |
| CTCTCCTCGACGCCTATTTG+CGG | 0.519190 | 1.4:-42098407 | None:intergenic |
| ATCCTATTATTTCTAAATCA+CGG | 0.522724 | 1.4:-42098490 | None:intergenic |
| AATAATAGGATACGATGAGA+AGG | 0.549854 | 1.4:+42098502 | MS.gene78633:CDS |
| TGATATCAATAGATTTGAAT+GGG | 0.559675 | 1.4:+42097993 | MS.gene78633:CDS |
| GAGTGTCGGTACCCAAACTC+TGG | 0.588931 | 1.4:+42098427 | MS.gene78633:CDS |
| GTCGGTACCCAAACTCTGGT+CGG | 0.618939 | 1.4:+42098431 | MS.gene78633:CDS |
| ATAGGCGTCGAGGAGAGTGT+CGG | 0.643134 | 1.4:+42098413 | MS.gene78633:CDS |
| AGCGATTTGACAGAGAATTG+GGG | 0.653308 | 1.4:+42098534 | MS.gene78633:CDS |
| GGATATGAATCCCAACCCAA+AGG | 0.674653 | 1.4:-42098557 | None:intergenic |
| TTTGCCGCAAATAGGCGTCG+AGG | 0.699807 | 1.4:+42098403 | MS.gene78633:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAAAATAGTAACGATAT+AGG | - | chr1.4:42098178-42098197 | None:intergenic | 20.0% |
| !! | ATAGAAAAGTAAGAAAGTAA+AGG | - | chr1.4:42098066-42098085 | None:intergenic | 20.0% |
| !! | TAGAAAAGTAAGAAAGTAAA+GGG | - | chr1.4:42098065-42098084 | None:intergenic | 20.0% |
| !! | TGATATCAATAGATTTGAAT+GGG | + | chr1.4:42097993-42098012 | MS.gene78633:CDS | 20.0% |
| !!! | ATCCTATTATTTCTAAATCA+CGG | - | chr1.4:42098493-42098512 | None:intergenic | 20.0% |
| ! | CTGATATCAATAGATTTGAA+TGG | + | chr1.4:42097992-42098011 | MS.gene78633:CDS | 25.0% |
| AATAATAGGATACGATGAGA+AGG | + | chr1.4:42098502-42098521 | MS.gene78633:CDS | 30.0% | |
| AGTAAGAAAGTAAAGGGTAA+TGG | - | chr1.4:42098059-42098078 | None:intergenic | 30.0% | |
| ATTGCTCAAAACGAATCTAT+TGG | + | chr1.4:42098084-42098103 | MS.gene78633:CDS | 30.0% | |
| GACCGTGATTTAGAAATAAT+AGG | + | chr1.4:42098488-42098507 | MS.gene78633:CDS | 30.0% | |
| TCAAAACATTGAACTTGGTT+AGG | + | chr1.4:42098235-42098254 | MS.gene78633:intron | 30.0% | |
| TGATGTCAAAACATTGAACT+TGG | + | chr1.4:42098230-42098249 | MS.gene78633:intron | 30.0% | |
| AAGCGATTTGACAGAGAATT+GGG | + | chr1.4:42098533-42098552 | MS.gene78633:CDS | 35.0% | |
| CAAGCTAAATATGTTGGAAC+TGG | + | chr1.4:42097964-42097983 | MS.gene78633:CDS | 35.0% | |
| CATCTGCAAGCTAAATATGT+TGG | + | chr1.4:42097958-42097977 | MS.gene78633:CDS | 35.0% | |
| GATAGCTATGCTTCGTATAT+TGG | + | chr1.4:42098033-42098052 | MS.gene78633:CDS | 35.0% | |
| TTGACACATCAACTTGTAGT+AGG | + | chr1.4:42098273-42098292 | MS.gene78633:intron | 35.0% | |
| !! | TAACGATATAGGCATTGAAC+AGG | - | chr1.4:42098167-42098186 | None:intergenic | 35.0% |
| AGCGATTTGACAGAGAATTG+GGG | + | chr1.4:42098534-42098553 | MS.gene78633:CDS | 40.0% | |
| CAAATAACCGACCAGAGTTT+GGG | - | chr1.4:42098441-42098460 | None:intergenic | 40.0% | |
| CAAGCGATTTGACAGAGAAT+TGG | + | chr1.4:42098532-42098551 | MS.gene78633:CDS | 40.0% | |
| TATTTCGCTATTGCCTGCTA+TGG | - | chr1.4:42098346-42098365 | None:intergenic | 40.0% | |
| TCTGGTCGGTTATTTGGAAA+TGG | + | chr1.4:42098445-42098464 | MS.gene78633:CDS | 40.0% | |
| ! | ACAGCGTTACACTTTTATGC+AGG | + | chr1.4:42098110-42098129 | MS.gene78633:CDS | 40.0% |
| AGCAGGCAATAGCGAAATAG+CGG | + | chr1.4:42098347-42098366 | MS.gene78633:intron | 45.0% | |
| CCAAACTCTGGTCGGTTATT+TGG | + | chr1.4:42098439-42098458 | MS.gene78633:CDS | 45.0% | |
| CCAAATAACCGACCAGAGTT+TGG | - | chr1.4:42098442-42098461 | None:intergenic | 45.0% | |
| GGATATGAATCCCAACCCAA+AGG | - | chr1.4:42098560-42098579 | None:intergenic | 45.0% | |
| GTCGGTTATTTGGAAATGGC+AGG | + | chr1.4:42098449-42098468 | MS.gene78633:CDS | 45.0% | |
| ! | CTAAAGCGTTTGCCGCAAAT+AGG | + | chr1.4:42098395-42098414 | MS.gene78633:CDS | 45.0% |
| AGCTTGCAGATGCTCGAGTT+GGG | - | chr1.4:42097950-42097969 | None:intergenic | 50.0% | |
| GACAGAGAATTGGGGCCTTT+GGG | + | chr1.4:42098542-42098561 | MS.gene78633:CDS | 50.0% | |
| TGACAGAGAATTGGGGCCTT+TGG | + | chr1.4:42098541-42098560 | MS.gene78633:CDS | 50.0% | |
| ! | AGAATTGGGGCCTTTGGGTT+GGG | + | chr1.4:42098547-42098566 | MS.gene78633:CDS | 50.0% |
| ! | GCAATAGCGAAATAGCGGAG+AGG | + | chr1.4:42098352-42098371 | MS.gene78633:intron | 50.0% |
| ! | GCGAAATAGCGGAGAGGATT+AGG | + | chr1.4:42098358-42098377 | MS.gene78633:intron | 50.0% |
| ! | TAGCTTGCAGATGCTCGAGT+TGG | - | chr1.4:42097951-42097970 | None:intergenic | 50.0% |
| CTCTCCTCGACGCCTATTTG+CGG | - | chr1.4:42098410-42098429 | None:intergenic | 55.0% | |
| GAGAATTGGGGCCTTTGGGT+TGG | + | chr1.4:42098546-42098565 | MS.gene78633:CDS | 55.0% | |
| GTCGGTACCCAAACTCTGGT+CGG | + | chr1.4:42098431-42098450 | MS.gene78633:CDS | 55.0% | |
| ! | TTTGCCGCAAATAGGCGTCG+AGG | + | chr1.4:42098403-42098422 | MS.gene78633:CDS | 55.0% |
| !! | ATAGGCGTCGAGGAGAGTGT+CGG | + | chr1.4:42098413-42098432 | MS.gene78633:CDS | 55.0% |
| !! | GAGTGTCGGTACCCAAACTC+TGG | + | chr1.4:42098427-42098446 | MS.gene78633:CDS | 55.0% |
| CTGTGCTGTGCTGCCATAGC+AGG | + | chr1.4:42098330-42098349 | MS.gene78633:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 42097916 | 42098592 | 42097916 | ID=MS.gene78633 |
| chr1.4 | mRNA | 42097916 | 42098592 | 42097916 | ID=MS.gene78633.t1;Parent=MS.gene78633 |
| chr1.4 | exon | 42097916 | 42098131 | 42097916 | ID=MS.gene78633.t1.exon1;Parent=MS.gene78633.t1 |
| chr1.4 | CDS | 42097916 | 42098131 | 42097916 | ID=cds.MS.gene78633.t1;Parent=MS.gene78633.t1 |
| chr1.4 | exon | 42098380 | 42098592 | 42098380 | ID=MS.gene78633.t1.exon2;Parent=MS.gene78633.t1 |
| chr1.4 | CDS | 42098380 | 42098592 | 42098380 | ID=cds.MS.gene78633.t1;Parent=MS.gene78633.t1 |
| Gene Sequence |
| Protein sequence |
