Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040992.t1 | XP_003628625.1 | 100 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 1.60E-33 | 151.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040992.t1 | P58728 | 90.3 | 72 | 7 | 0 | 1 | 72 | 1 | 72 | 1.8e-33 | 142.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040992.t1 | G7IW51 | 100.0 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 1.2e-33 | 151.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene78633 | MS.gene040992 | PPI |
| MS.gene039781 | MS.gene040992 | PPI |
| MS.gene65850 | MS.gene040992 | PPI |
| MS.gene85479 | MS.gene040992 | PPI |
| MS.gene018837 | MS.gene040992 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040992.t1 | MTR_8g062750 | 100.000 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 3.22e-50 | 152 |
| MS.gene040992.t1 | MTR_3g019310 | 100.000 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 3.36e-50 | 152 |
| MS.gene040992.t1 | MTR_3g019300 | 100.000 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 3.36e-50 | 152 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene040992.t1 | AT4G14342 | 90.278 | 72 | 7 | 0 | 1 | 72 | 1 | 72 | 8.99e-47 | 144 |
| MS.gene040992.t1 | AT4G14342 | 90.278 | 72 | 7 | 0 | 1 | 72 | 1 | 72 | 9.15e-47 | 143 |
| MS.gene040992.t1 | AT3G23325 | 88.889 | 72 | 8 | 0 | 1 | 72 | 1 | 72 | 5.02e-46 | 142 |
Find 12 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATAGCTATGCTTCGTATAT+TGG | 0.282004 | 1.1:-38901039 | MS.gene040992:CDS |
| ATTGCTCAAAACGAATCTAT+TGG | 0.369668 | 1.1:-38900988 | MS.gene040992:CDS |
| TAGAAAAGTAAGAAAGTAAA+GGG | 0.419141 | 1.1:+38901010 | None:intergenic |
| GCAATATGCAGGCGAGTGAT+AGG | 0.420071 | 1.1:-38901161 | None:intergenic |
| CAAGCTAAATATGTTGGAAC+TGG | 0.450379 | 1.1:-38901108 | MS.gene040992:CDS |
| ATAGAAAAGTAAGAAAGTAA+AGG | 0.454525 | 1.1:+38901009 | None:intergenic |
| CTGATATCAATAGATTTGAA+TGG | 0.473717 | 1.1:-38901080 | MS.gene040992:CDS |
| AGCTTGCAGATGCTCGAGTT+GGG | 0.481702 | 1.1:+38901125 | None:intergenic |
| AGTAAGAAAGTAAAGGGTAA+TGG | 0.502423 | 1.1:+38901016 | None:intergenic |
| TAGCTTGCAGATGCTCGAGT+TGG | 0.512932 | 1.1:+38901124 | None:intergenic |
| CATCTGCAAGCTAAATATGT+TGG | 0.518362 | 1.1:-38901114 | MS.gene040992:CDS |
| TGATATCAATAGATTTGAAT+GGG | 0.559675 | 1.1:-38901079 | MS.gene040992:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAGAAAAGTAAGAAAGTAA+AGG | + | chr1.1:38901113-38901132 | None:intergenic | 20.0% |
| !! | TAGAAAAGTAAGAAAGTAAA+GGG | + | chr1.1:38901112-38901131 | None:intergenic | 20.0% |
| !! | TGATATCAATAGATTTGAAT+GGG | - | chr1.1:38901040-38901059 | MS.gene040992:CDS | 20.0% |
| ! | CTGATATCAATAGATTTGAA+TGG | - | chr1.1:38901039-38901058 | MS.gene040992:CDS | 25.0% |
| AGTAAGAAAGTAAAGGGTAA+TGG | + | chr1.1:38901106-38901125 | None:intergenic | 30.0% | |
| ATTGCTCAAAACGAATCTAT+TGG | - | chr1.1:38901131-38901150 | MS.gene040992:CDS | 30.0% | |
| CAAGCTAAATATGTTGGAAC+TGG | - | chr1.1:38901011-38901030 | MS.gene040992:CDS | 35.0% | |
| CATCTGCAAGCTAAATATGT+TGG | - | chr1.1:38901005-38901024 | MS.gene040992:CDS | 35.0% | |
| GATAGCTATGCTTCGTATAT+TGG | - | chr1.1:38901080-38901099 | MS.gene040992:CDS | 35.0% | |
| AGCTTGCAGATGCTCGAGTT+GGG | + | chr1.1:38900997-38901016 | None:intergenic | 50.0% | |
| ! | TAGCTTGCAGATGCTCGAGT+TGG | + | chr1.1:38900998-38901017 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 38900963 | 38901178 | 38900963 | ID=MS.gene040992 |
| chr1.1 | mRNA | 38900963 | 38901178 | 38900963 | ID=MS.gene040992.t1;Parent=MS.gene040992 |
| chr1.1 | exon | 38900963 | 38901178 | 38900963 | ID=MS.gene040992.t1.exon1;Parent=MS.gene040992.t1 |
| chr1.1 | CDS | 38900963 | 38901178 | 38900963 | ID=cds.MS.gene040992.t1;Parent=MS.gene040992.t1 |
| Gene Sequence |
| Protein sequence |
