Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene039781.t1 | XP_003628625.1 | 98.6 | 73 | 1 | 0 | 1 | 73 | 1 | 73 | 1.70E-33 | 152.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene039781.t1 | P58728 | 89.0 | 73 | 8 | 0 | 1 | 73 | 1 | 73 | 1.9e-33 | 143.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene039781.t1 | G7IW51 | 98.6 | 73 | 1 | 0 | 1 | 73 | 1 | 73 | 1.2e-33 | 152.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene039781 | MS.gene040992 | PPI |
| MS.gene78633 | MS.gene039781 | PPI |
| MS.gene65850 | MS.gene039781 | PPI |
| MS.gene018837 | MS.gene039781 | PPI |
| MS.gene85479 | MS.gene039781 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene039781.t1 | MTR_3g019310 | 87.952 | 83 | 10 | 0 | 1 | 83 | 1 | 83 | 2.36e-50 | 155 |
| MS.gene039781.t1 | MTR_3g019300 | 87.952 | 83 | 10 | 0 | 1 | 83 | 1 | 83 | 2.36e-50 | 155 |
| MS.gene039781.t1 | MTR_8g062750 | 87.952 | 83 | 10 | 0 | 1 | 83 | 1 | 83 | 2.58e-50 | 155 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene039781.t1 | AT4G14342 | 71.875 | 96 | 25 | 1 | 1 | 96 | 1 | 94 | 2.93e-47 | 147 |
| MS.gene039781.t1 | AT4G14342 | 79.518 | 83 | 17 | 0 | 1 | 83 | 1 | 83 | 1.61e-46 | 145 |
| MS.gene039781.t1 | AT3G23325 | 77.273 | 88 | 19 | 1 | 1 | 88 | 1 | 87 | 4.13e-46 | 144 |
Find 28 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAATAACCGGCCACATTT+TGG | 0.104989 | 3.3:+11826844 | None:intergenic |
| CCAAAATGTGGCCGGTTATT+TGG | 0.135000 | 3.3:-11826844 | MS.gene039781:CDS |
| GATAGCTATGCTTCGTATAT+TGG | 0.282004 | 3.3:-11827269 | MS.gene039781:CDS |
| CTTAAGAGTTTGCCACAAAT+AGG | 0.364417 | 3.3:-11826891 | MS.gene039781:CDS |
| ATTGCTCAAAACGAATCTAT+TGG | 0.369668 | 3.3:-11827218 | MS.gene039781:CDS |
| ACCTACCATTTCCAAATAAC+CGG | 0.395116 | 3.3:+11826833 | None:intergenic |
| TCCTCATCGCCGCCTATTTG+TGG | 0.395661 | 3.3:+11826879 | None:intergenic |
| TAGAAAAGTAAGAAAGTAAA+GGG | 0.419141 | 3.3:+11827240 | None:intergenic |
| GCAATATGCAGGCGAGTGAT+AGG | 0.420071 | 3.3:-11827391 | None:intergenic |
| TGTGGCCGGTTATTTGGAAA+TGG | 0.423394 | 3.3:-11826838 | MS.gene039781:CDS |
| GACCGTGATTTAGAAATAAT+AGG | 0.443906 | 3.3:-11826795 | MS.gene039781:CDS |
| CAAGCTAAATATGTTGGAAC+TGG | 0.450379 | 3.3:-11827338 | MS.gene039781:CDS |
| ATAGAAAAGTAAGAAAGTAA+AGG | 0.454525 | 3.3:+11827239 | None:intergenic |
| CTGATATCAACAGATTTGAA+TGG | 0.473717 | 3.3:-11827310 | MS.gene039781:CDS |
| AGCTTGCAGATGCTCGAGTT+GGG | 0.481702 | 3.3:+11827355 | None:intergenic |
| AGTAAGAAAGTAAAGGGTAA+TGG | 0.502423 | 3.3:+11827246 | None:intergenic |
| TAGCTTGCAGATGCTCGAGT+TGG | 0.512932 | 3.3:+11827354 | None:intergenic |
| CATCTGCAAGCTAAATATGT+TGG | 0.518362 | 3.3:-11827344 | MS.gene039781:CDS |
| ATCCTATTATTTCTAAATCA+CGG | 0.522724 | 3.3:+11826793 | None:intergenic |
| AATAATAGGATACGATAAGA+AGG | 0.522758 | 3.3:-11826781 | MS.gene039781:CDS |
| TGATATCAACAGATTTGAAT+GGG | 0.559675 | 3.3:-11827309 | MS.gene039781:CDS |
| AATGGTAGGTTTAGCTAGAG+AGG | 0.564557 | 3.3:-11826820 | MS.gene039781:CDS |
| GGCGGCGATGAGGAGAGTGT+TGG | 0.579513 | 3.3:-11826870 | MS.gene039781:CDS |
| GTTGGAACCCAAAATGTGGC+CGG | 0.597376 | 3.3:-11826852 | MS.gene039781:CDS |
| GCCGGTTATTTGGAAATGGT+AGG | 0.609127 | 3.3:-11826834 | MS.gene039781:CDS |
| GAGTGTTGGAACCCAAAATG+TGG | 0.621945 | 3.3:-11826856 | MS.gene039781:CDS |
| AAGAGTTTGCCACAAATAGG+CGG | 0.641707 | 3.3:-11826888 | MS.gene039781:CDS |
| GCCACAAATAGGCGGCGATG+AGG | 0.649788 | 3.3:-11826880 | MS.gene039781:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAGAAAAGTAAGAAAGTAA+AGG | + | chr3.3:11826917-11826936 | None:intergenic | 20.0% |
| !! | TAGAAAAGTAAGAAAGTAAA+GGG | + | chr3.3:11826916-11826935 | None:intergenic | 20.0% |
| !!! | ATCCTATTATTTCTAAATCA+CGG | + | chr3.3:11827363-11827382 | None:intergenic | 20.0% |
| ! | AAAGAAAATAGTAACGATCT+AGG | + | chr3.3:11827029-11827048 | None:intergenic | 25.0% |
| ! | AATAATAGGATACGATAAGA+AGG | - | chr3.3:11827372-11827391 | MS.gene039781:CDS | 25.0% |
| ! | TGATATCAACAGATTTGAAT+GGG | - | chr3.3:11826844-11826863 | MS.gene039781:CDS | 25.0% |
| AGTAAGAAAGTAAAGGGTAA+TGG | + | chr3.3:11826910-11826929 | None:intergenic | 30.0% | |
| ATTGCTCAAAACGAATCTAT+TGG | - | chr3.3:11826935-11826954 | MS.gene039781:intron | 30.0% | |
| CTGATATCAACAGATTTGAA+TGG | - | chr3.3:11826843-11826862 | MS.gene039781:CDS | 30.0% | |
| GACCGTGATTTAGAAATAAT+AGG | - | chr3.3:11827358-11827377 | MS.gene039781:CDS | 30.0% | |
| TCAAAACATTGAACTTGGTT+AGG | - | chr3.3:11827086-11827105 | MS.gene039781:intron | 30.0% | |
| TGATGTCAAAACATTGAACT+TGG | - | chr3.3:11827081-11827100 | MS.gene039781:intron | 30.0% | |
| ACCTACCATTTCCAAATAAC+CGG | + | chr3.3:11827323-11827342 | None:intergenic | 35.0% | |
| CAAGCTAAATATGTTGGAAC+TGG | - | chr3.3:11826815-11826834 | MS.gene039781:CDS | 35.0% | |
| CATCTGCAAGCTAAATATGT+TGG | - | chr3.3:11826809-11826828 | MS.gene039781:CDS | 35.0% | |
| CTTAAGAGTTTGCCACAAAT+AGG | - | chr3.3:11827262-11827281 | MS.gene039781:CDS | 35.0% | |
| GATAGCTATGCTTCGTATAT+TGG | - | chr3.3:11826884-11826903 | MS.gene039781:CDS | 35.0% | |
| TTGACACATCAACATGTAGT+AGG | - | chr3.3:11827124-11827143 | MS.gene039781:intron | 35.0% | |
| AAGAGTTTGCCACAAATAGG+CGG | - | chr3.3:11827265-11827284 | MS.gene039781:CDS | 40.0% | |
| AATGGTAGGTTTAGCTAGAG+AGG | - | chr3.3:11827333-11827352 | MS.gene039781:CDS | 40.0% | |
| TATTTCGCTATTGCCTGCTA+AGG | + | chr3.3:11827213-11827232 | None:intergenic | 40.0% | |
| ! | ACAGCGTTACACTTTTATGC+AGG | - | chr3.3:11826961-11826980 | MS.gene039781:intron | 40.0% |
| ! | CAAATAACCGGCCACATTTT+GGG | + | chr3.3:11827311-11827330 | None:intergenic | 40.0% |
| CCAAAATGTGGCCGGTTATT+TGG | - | chr3.3:11827309-11827328 | MS.gene039781:CDS | 45.0% | |
| GCAATAGCGAAATAGCGTAG+AGG | - | chr3.3:11827219-11827238 | MS.gene039781:CDS | 45.0% | |
| GCCGGTTATTTGGAAATGGT+AGG | - | chr3.3:11827319-11827338 | MS.gene039781:CDS | 45.0% | |
| GCGAAATAGCGTAGAGGATT+AGG | - | chr3.3:11827225-11827244 | MS.gene039781:CDS | 45.0% | |
| TGTGGCCGGTTATTTGGAAA+TGG | - | chr3.3:11827315-11827334 | MS.gene039781:CDS | 45.0% | |
| ! | CCAAATAACCGGCCACATTT+TGG | + | chr3.3:11827312-11827331 | None:intergenic | 45.0% |
| !! | GAGTGTTGGAACCCAAAATG+TGG | - | chr3.3:11827297-11827316 | MS.gene039781:CDS | 45.0% |
| AGCTTGCAGATGCTCGAGTT+GGG | + | chr3.3:11826801-11826820 | None:intergenic | 50.0% | |
| GTTGGAACCCAAAATGTGGC+CGG | - | chr3.3:11827301-11827320 | MS.gene039781:CDS | 50.0% | |
| ! | TAGCTTGCAGATGCTCGAGT+TGG | + | chr3.3:11826802-11826821 | None:intergenic | 50.0% |
| CTGTGATGTGCTGCCTTAGC+AGG | - | chr3.3:11827197-11827216 | MS.gene039781:CDS | 55.0% | |
| TCCTCATCGCCGCCTATTTG+TGG | + | chr3.3:11827277-11827296 | None:intergenic | 55.0% | |
| GCCACAAATAGGCGGCGATG+AGG | - | chr3.3:11827273-11827292 | MS.gene039781:CDS | 60.0% | |
| ! | GGCGGCGATGAGGAGAGTGT+TGG | - | chr3.3:11827283-11827302 | MS.gene039781:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 11826767 | 11827408 | 11826767 | ID=MS.gene039781 |
| chr3.3 | mRNA | 11826767 | 11827408 | 11826767 | ID=MS.gene039781.t1;Parent=MS.gene039781 |
| chr3.3 | exon | 11827193 | 11827408 | 11827193 | ID=MS.gene039781.t1.exon1;Parent=MS.gene039781.t1 |
| chr3.3 | CDS | 11827193 | 11827408 | 11827193 | ID=cds.MS.gene039781.t1;Parent=MS.gene039781.t1 |
| chr3.3 | exon | 11826767 | 11826928 | 11826767 | ID=MS.gene039781.t1.exon2;Parent=MS.gene039781.t1 |
| chr3.3 | CDS | 11826767 | 11826928 | 11826767 | ID=cds.MS.gene039781.t1;Parent=MS.gene039781.t1 |
| Gene Sequence |
| Protein sequence |
