Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65850.t1 | XP_003628625.1 | 94.8 | 77 | 4 | 0 | 1 | 77 | 1 | 77 | 1.30E-33 | 152.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65850.t1 | P58728 | 85.7 | 77 | 11 | 0 | 1 | 77 | 1 | 77 | 1.5e-33 | 143.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65850.t1 | G7IW51 | 94.8 | 77 | 4 | 0 | 1 | 77 | 1 | 77 | 9.7e-34 | 152.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene65850 | MS.gene018837 | PPI |
| MS.gene78633 | MS.gene65850 | PPI |
| MS.gene65850 | MS.gene040992 | PPI |
| MS.gene65850 | MS.gene039781 | PPI |
| MS.gene65850 | MS.gene85479 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65850.t1 | MTR_3g019310 | 93.590 | 78 | 5 | 0 | 1 | 78 | 1 | 78 | 3.31e-50 | 153 |
| MS.gene65850.t1 | MTR_3g019300 | 93.590 | 78 | 5 | 0 | 1 | 78 | 1 | 78 | 3.31e-50 | 153 |
| MS.gene65850.t1 | MTR_8g062750 | 93.590 | 78 | 5 | 0 | 1 | 78 | 1 | 78 | 4.17e-50 | 153 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65850.t1 | AT4G14342 | 84.615 | 78 | 12 | 0 | 1 | 78 | 1 | 78 | 1.89e-46 | 144 |
| MS.gene65850.t1 | AT4G14342 | 90.278 | 72 | 7 | 0 | 1 | 72 | 1 | 72 | 3.35e-46 | 144 |
| MS.gene65850.t1 | AT3G23325 | 83.333 | 78 | 13 | 0 | 1 | 78 | 1 | 78 | 9.79e-46 | 142 |
Find 21 sgRNAs with CRISPR-Local
Find 121 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATAGCTATGCTTCGTATAT+TGG | 0.282004 | 3.2:-9713041 | MS.gene65850:CDS |
| TCTAGAAGAGATTGACGATT+AGG | 0.360918 | 3.2:-9711529 | None:intergenic |
| ATTGCTCAAAACGAATCTAT+TGG | 0.369668 | 3.2:-9712990 | MS.gene65850:CDS |
| TAGAAAAGTAAGAAAGTAAA+GGG | 0.419141 | 3.2:+9713012 | None:intergenic |
| GCAATATGCAGGCGAGTGAT+AGG | 0.420071 | 3.2:-9713163 | None:intergenic |
| AACATAATGTTCAAAGGGAC+TGG | 0.429825 | 3.2:+9711584 | None:intergenic |
| CAAGCTAAATATGTTGGAAC+TGG | 0.450379 | 3.2:-9713110 | MS.gene65850:CDS |
| ATAGAAAAGTAAGAAAGTAA+AGG | 0.454525 | 3.2:+9713011 | None:intergenic |
| CTGATATCAATAGATTTGAA+TGG | 0.473717 | 3.2:-9713082 | MS.gene65850:CDS |
| AGCTTGCAGATGCTCGAGTT+GGG | 0.481702 | 3.2:+9713127 | None:intergenic |
| CTCTATAACATAATGTTCAA+AGG | 0.485678 | 3.2:+9711578 | None:intergenic |
| AGTAAGAAAGTAAAGGGTAA+TGG | 0.502423 | 3.2:+9713018 | None:intergenic |
| TCTATAACATAATGTTCAAA+GGG | 0.504261 | 3.2:+9711579 | None:intergenic |
| TAGCTTGCAGATGCTCGAGT+TGG | 0.512932 | 3.2:+9713126 | None:intergenic |
| CATCTGCAAGCTAAATATGT+TGG | 0.518362 | 3.2:-9713116 | MS.gene65850:CDS |
| TGATATCAATAGATTTGAAT+GGG | 0.559675 | 3.2:-9713081 | MS.gene65850:CDS |
| ACATAATGTTCAAAGGGACT+GGG | 0.575136 | 3.2:+9711585 | None:intergenic |
| TCGTCAATCTCTTCTAGAGA+AGG | 0.578705 | 3.2:+9711534 | None:intergenic |
| ACATTATGTTATAGAGCATG+AGG | 0.592176 | 3.2:-9711571 | MS.gene65850:CDS |
| CATTATGTTATAGAGCATGA+GGG | 0.671221 | 3.2:-9711570 | MS.gene65850:CDS |
| TATGTTATAGAGCATGAGGG+TGG | 0.693222 | 3.2:-9711567 | MS.gene65850:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCCTATTATTTATAAATCA+CGG | + | chr3.2:9712097-9712116 | None:intergenic | 15.0% |
| !!! | ATTAATATTTGAGTTTAAGA+AGG | - | chr3.2:9712242-9712261 | MS.gene65850:intron | 15.0% |
| !!! | TTAATATTTGAGTTTAAGAA+GGG | - | chr3.2:9712243-9712262 | MS.gene65850:intron | 15.0% |
| !! | AAAGAAAATAGTAACGATAT+AGG | + | chr3.2:9711792-9711811 | None:intergenic | 20.0% |
| !! | ATAGAAAAGTAAGAAAGTAA+AGG | + | chr3.2:9711680-9711699 | None:intergenic | 20.0% |
| !! | TAGAAAAGTAAGAAAGTAAA+GGG | + | chr3.2:9711679-9711698 | None:intergenic | 20.0% |
| !! | TCTATAACATAATGTTCAAA+GGG | + | chr3.2:9713112-9713131 | None:intergenic | 20.0% |
| !! | TGATATCAATAGATTTGAAT+GGG | - | chr3.2:9711607-9711626 | MS.gene65850:intron | 20.0% |
| !!! | AAATCAGATTTTGTTCAAAT+TGG | - | chr3.2:9712585-9712604 | MS.gene65850:intron | 20.0% |
| !!! | AATCAGATTTTGTTCAAATT+GGG | - | chr3.2:9712586-9712605 | MS.gene65850:intron | 20.0% |
| !!! | AGAAAACTAGTTTAGAAATA+TGG | - | chr3.2:9712746-9712765 | MS.gene65850:intron | 20.0% |
| !!! | AGTTTAGAAATATGGTTTTT+TGG | - | chr3.2:9712754-9712773 | MS.gene65850:intron | 20.0% |
| !!! | GGTATTTTATTAGAAAGAAT+GGG | + | chr3.2:9712187-9712206 | None:intergenic | 20.0% |
| !!! | GTATTTTATTAGAAAGAATG+GGG | + | chr3.2:9712186-9712205 | None:intergenic | 20.0% |
| !!! | TGGTATTTTATTAGAAAGAA+TGG | + | chr3.2:9712188-9712207 | None:intergenic | 20.0% |
| !!! | TTCAATTCTTTTCTTTGTTT+TGG | - | chr3.2:9712359-9712378 | MS.gene65850:intron | 20.0% |
| ! | AAACTAGTGACAAAAATCTT+TGG | + | chr3.2:9712414-9712395 | None:intergenic | 25.0% |
| ! | ATCCTTCTTAAACATGTTAT+TGG | + | chr3.2:9713028-9713047 | None:intergenic | 25.0% |
| ! | ATGTCTCATCTAATAATGAA+TGG | - | chr3.2:9713059-9713078 | MS.gene65850:CDS | 25.0% |
| ! | CTCTATAACATAATGTTCAA+AGG | + | chr3.2:9713113-9713132 | None:intergenic | 25.0% |
| ! | CTGATATCAATAGATTTGAA+TGG | - | chr3.2:9711606-9711625 | MS.gene65850:intron | 25.0% |
| ! | GACCGTGATTTATAAATAAT+AGG | - | chr3.2:9712092-9712111 | MS.gene65850:intron | 25.0% |
| ! | TACCAATAACATGTTTAAGA+AGG | - | chr3.2:9713023-9713042 | MS.gene65850:CDS | 25.0% |
| !! | CCTTCTCTTAAAAATGTTTT+TGG | + | chr3.2:9712986-9713005 | None:intergenic | 25.0% |
| !! | TTAAACATGTTATTGGTAGT+GGG | + | chr3.2:9713021-9713040 | None:intergenic | 25.0% |
| !!! | AACTTTTTCAGACTCTTTTT+TGG | - | chr3.2:9712295-9712314 | MS.gene65850:intron | 25.0% |
| !!! | CCAAAAACATTTTTAAGAGA+AGG | - | chr3.2:9712983-9713002 | MS.gene65850:CDS | 25.0% |
| !!! | TAATAATGAATGGATTGTGA+AGG | - | chr3.2:9713069-9713088 | MS.gene65850:CDS | 25.0% |
| !!! | TTTTTTGGTTGTTAACCAAA+TGG | - | chr3.2:9712769-9712788 | MS.gene65850:intron | 25.0% |
| ACATTATGTTATAGAGCATG+AGG | - | chr3.2:9713117-9713136 | MS.gene65850:CDS | 30.0% | |
| AGAAAAATCCTACAAAGTCT+TGG | - | chr3.2:9712889-9712908 | MS.gene65850:intron | 30.0% | |
| AGTAAGAAAGTAAAGGGTAA+TGG | + | chr3.2:9711673-9711692 | None:intergenic | 30.0% | |
| ATAATAGGATAACGATGAGA+AGG | - | chr3.2:9712107-9712126 | MS.gene65850:intron | 30.0% | |
| ATTGCTCAAAACGAATCTAT+TGG | - | chr3.2:9711698-9711717 | MS.gene65850:intron | 30.0% | |
| CATTATGTTATAGAGCATGA+GGG | - | chr3.2:9713118-9713137 | MS.gene65850:CDS | 30.0% | |
| CTTAAACATGTTATTGGTAG+TGG | + | chr3.2:9713022-9713041 | None:intergenic | 30.0% | |
| GCTTAAGAGAGCTAATAATA+TGG | + | chr3.2:9712483-9712502 | None:intergenic | 30.0% | |
| TCAAAACATTGAACTTGGTT+AGG | - | chr3.2:9711849-9711868 | MS.gene65850:intron | 30.0% | |
| TGATGTCAAAACATTGAACT+TGG | - | chr3.2:9711844-9711863 | MS.gene65850:intron | 30.0% | |
| TGGTAACACTTCAATCAAAA+AGG | + | chr3.2:9712463-9712482 | None:intergenic | 30.0% | |
| TGTACAATTGTGTGATTGTA+TGG | + | chr3.2:9712531-9712550 | None:intergenic | 30.0% | |
| ! | AGTTTTTGCGATTCTGATTA+GGG | - | chr3.2:9712658-9712677 | MS.gene65850:intron | 30.0% |
| ! | ATTATTAGCTCTCTTAAGCA+TGG | - | chr3.2:9712484-9712503 | MS.gene65850:intron | 30.0% |
| ! | GGATTTTTCTTTTATAGCGA+AGG | + | chr3.2:9712879-9712898 | None:intergenic | 30.0% |
| !! | AAAAAGAGAAAGCATTGAAG+TGG | - | chr3.2:9712942-9712961 | MS.gene65850:intron | 30.0% |
| !! | CTTGTTTCTATAGCTAATGA+CGG | + | chr3.2:9712846-9712865 | None:intergenic | 30.0% |
| !! | TTGTTTCTATAGCTAATGAC+GGG | + | chr3.2:9712845-9712864 | None:intergenic | 30.0% |
| AACATAATGTTCAAAGGGAC+TGG | + | chr3.2:9713107-9713126 | None:intergenic | 35.0% | |
| AAGAGTCTGAAAAAGTTCTG+AGG | + | chr3.2:9712292-9712311 | None:intergenic | 35.0% | |
| AAGCGATTTGACAGAGAATT+GGG | - | chr3.2:9712138-9712157 | MS.gene65850:intron | 35.0% | |
| AATACCAGATTTCTACCAGT+AGG | - | chr3.2:9712201-9712220 | MS.gene65850:intron | 35.0% | |
| AATCGTTGAATTGAGTGATC+TGG | - | chr3.2:9712625-9712644 | MS.gene65850:intron | 35.0% | |
| ACATAATGTTCAAAGGGACT+GGG | + | chr3.2:9713106-9713125 | None:intergenic | 35.0% | |
| ATACCAGATTTCTACCAGTA+GGG | - | chr3.2:9712202-9712221 | MS.gene65850:intron | 35.0% | |
| CAAGCTAAATATGTTGGAAC+TGG | - | chr3.2:9711578-9711597 | MS.gene65850:CDS | 35.0% | |
| CATCTGCAAGCTAAATATGT+TGG | - | chr3.2:9711572-9711591 | MS.gene65850:CDS | 35.0% | |
| GATAGCTATGCTTCGTATAT+TGG | - | chr3.2:9711647-9711666 | MS.gene65850:intron | 35.0% | |
| TCAAATTCTCATGTTGCTAC+TGG | - | chr3.2:9712794-9712813 | MS.gene65850:intron | 35.0% | |
| TCCTACAAAGTCTTGGAAAT+AGG | - | chr3.2:9712896-9712915 | MS.gene65850:intron | 35.0% | |
| TCCTATTTCCAAGACTTTGT+AGG | + | chr3.2:9712900-9712919 | None:intergenic | 35.0% | |
| TTCAAACAATCCCCAAATTG+TGG | + | chr3.2:9712323-9712342 | None:intergenic | 35.0% | |
| ! | AAGAACCTGTTTTCTTCTTG+CGG | - | chr3.2:9712717-9712736 | MS.gene65850:intron | 35.0% |
| ! | CAGATTTTGTTCAAATTGGG+TGG | - | chr3.2:9712589-9712608 | MS.gene65850:intron | 35.0% |
| ! | CATGAGAATTTGAAGCCATT+TGG | + | chr3.2:9712787-9712806 | None:intergenic | 35.0% |
| ! | GAGTTTTTGCGATTCTGATT+AGG | - | chr3.2:9712657-9712676 | MS.gene65850:intron | 35.0% |
| ! | GTTTTTGCGATTCTGATTAG+GGG | - | chr3.2:9712659-9712678 | MS.gene65850:intron | 35.0% |
| !!! | CTTTTTTGGCACCACAATTT+GGG | - | chr3.2:9712309-9712328 | MS.gene65850:intron | 35.0% |
| !!! | TCTTTTTTGGCACCACAATT+TGG | - | chr3.2:9712308-9712327 | MS.gene65850:intron | 35.0% |
| !!! | TTTTTTGGCACCACAATTTG+GGG | - | chr3.2:9712310-9712329 | MS.gene65850:intron | 35.0% |
| AGATTTCTACCAGTAGGGAA+GGG | - | chr3.2:9712207-9712226 | MS.gene65850:intron | 40.0% | |
| AGCGATTTGACAGAGAATTG+GGG | - | chr3.2:9712139-9712158 | MS.gene65850:intron | 40.0% | |
| AGTTTAAGAAGGGAACAGAG+GGG | - | chr3.2:9712253-9712272 | MS.gene65850:intron | 40.0% | |
| CAAATAACCGACCAGAGTTT+GGG | + | chr3.2:9712045-9712064 | None:intergenic | 40.0% | |
| CAAGCGATTTGACAGAGAAT+TGG | - | chr3.2:9712137-9712156 | MS.gene65850:intron | 40.0% | |
| CTCAATTCAACGATTGACAG+TGG | + | chr3.2:9712620-9712639 | None:intergenic | 40.0% | |
| GAGTTTAAGAAGGGAACAGA+GGG | - | chr3.2:9712252-9712271 | MS.gene65850:intron | 40.0% | |
| TATGTTATAGAGCATGAGGG+TGG | - | chr3.2:9713121-9713140 | MS.gene65850:CDS | 40.0% | |
| TATTTCGCTATTGCCTGCTA+TGG | + | chr3.2:9711950-9711969 | None:intergenic | 40.0% | |
| TCTGGTCGGTTATTTGGAAA+TGG | - | chr3.2:9712049-9712068 | MS.gene65850:intron | 40.0% | |
| TGAGTTTAAGAAGGGAACAG+AGG | - | chr3.2:9712251-9712270 | MS.gene65850:intron | 40.0% | |
| TTGAGTGATCTGGATCTTTC+TGG | - | chr3.2:9712635-9712654 | MS.gene65850:intron | 40.0% | |
| ! | ACAGCGTTACACTTTTATGC+AGG | - | chr3.2:9711724-9711743 | MS.gene65850:intron | 40.0% |
| ! | AGAACCTGTTTTCTTCTTGC+GGG | - | chr3.2:9712718-9712737 | MS.gene65850:intron | 40.0% |
| ! | TCGTCAATCTCTTCTAGAGA+AGG | + | chr3.2:9713157-9713176 | None:intergenic | 40.0% |
| !! | AAGAGAAAGCATTGAAGTGG+AGG | - | chr3.2:9712945-9712964 | MS.gene65850:intron | 40.0% |
| !! | AGAGAAAGCATTGAAGTGGA+GGG | - | chr3.2:9712946-9712965 | MS.gene65850:intron | 40.0% |
| AGCAGGCAATAGCGAAATAG+CGG | - | chr3.2:9711951-9711970 | MS.gene65850:intron | 45.0% | |
| CAGATTTCTACCAGTAGGGA+AGG | - | chr3.2:9712206-9712225 | MS.gene65850:intron | 45.0% | |
| CCAAACTCTGGTCGGTTATT+TGG | - | chr3.2:9712043-9712062 | MS.gene65850:intron | 45.0% | |
| CCAAATAACCGACCAGAGTT+TGG | + | chr3.2:9712046-9712065 | None:intergenic | 45.0% | |
| CTAACCCGCAAGAAGAAAAC+AGG | + | chr3.2:9712725-9712744 | None:intergenic | 45.0% | |
| CTTCCCTACTGGTAGAAATC+TGG | + | chr3.2:9712208-9712227 | None:intergenic | 45.0% | |
| GGATATGAATCCCAACCCAA+AGG | + | chr3.2:9712165-9712184 | None:intergenic | 45.0% | |
| GTCGGTTATTTGGAAATGGC+AGG | - | chr3.2:9712053-9712072 | MS.gene65850:intron | 45.0% | |
| TACAACTTTGCTTGCCCTCA+GGG | - | chr3.2:9712547-9712566 | MS.gene65850:intron | 45.0% | |
| TTAGCACTCTTAGTCCCTGA+GGG | + | chr3.2:9712564-9712583 | None:intergenic | 45.0% | |
| TTCTCATGTTGCTACTGGTG+AGG | - | chr3.2:9712799-9712818 | MS.gene65850:intron | 45.0% | |
| TTTAGCACTCTTAGTCCCTG+AGG | + | chr3.2:9712565-9712584 | None:intergenic | 45.0% | |
| TTTGCGATTCTGATTAGGGG+TGG | - | chr3.2:9712662-9712681 | MS.gene65850:intron | 45.0% | |
| AGCTTGCAGATGCTCGAGTT+GGG | + | chr3.2:9711564-9711583 | None:intergenic | 50.0% | |
| GACAGAGAATTGGGGCCTTT+GGG | - | chr3.2:9712147-9712166 | MS.gene65850:intron | 50.0% | |
| GTACAACTTTGCTTGCCCTC+AGG | - | chr3.2:9712546-9712565 | MS.gene65850:intron | 50.0% | |
| GTTGGTACCCAAACTCTGGT+CGG | - | chr3.2:9712035-9712054 | MS.gene65850:intron | 50.0% | |
| TGACAGAGAATTGGGGCCTT+TGG | - | chr3.2:9712146-9712165 | MS.gene65850:intron | 50.0% | |
| ! | AGAATTGGGGCCTTTGGGTT+GGG | - | chr3.2:9712152-9712171 | MS.gene65850:intron | 50.0% |
| ! | CTAAAGCGTTCGCCGCAAAT+AGG | - | chr3.2:9711999-9712018 | MS.gene65850:intron | 50.0% |
| ! | GCAATAGCGAAATAGCGGAG+AGG | - | chr3.2:9711956-9711975 | MS.gene65850:intron | 50.0% |
| ! | GCGAAATAGCGGAGAGGATT+AGG | - | chr3.2:9711962-9711981 | MS.gene65850:intron | 50.0% |
| ! | TAGCTTGCAGATGCTCGAGT+TGG | + | chr3.2:9711565-9711584 | None:intergenic | 50.0% |
| !! | GAGTGTTGGTACCCAAACTC+TGG | - | chr3.2:9712031-9712050 | MS.gene65850:intron | 50.0% |
| AAGAAGGGAACAGAGGGGCA+TGG | - | chr3.2:9712258-9712277 | MS.gene65850:intron | 55.0% | |
| CCAGTAGGGAAGGGAAAGAG+AGG | - | chr3.2:9712216-9712235 | MS.gene65850:intron | 55.0% | |
| CCTCTCTTTCCCTTCCCTAC+TGG | + | chr3.2:9712219-9712238 | None:intergenic | 55.0% | |
| CTCTCCTCGACGCCTATTTG+CGG | + | chr3.2:9712014-9712033 | None:intergenic | 55.0% | |
| GAGAATTGGGGCCTTTGGGT+TGG | - | chr3.2:9712151-9712170 | MS.gene65850:intron | 55.0% | |
| GCGATTCTGATTAGGGGTGG+TGG | - | chr3.2:9712665-9712684 | MS.gene65850:intron | 55.0% | |
| GTCGCATGACGCACATGCTA+GGG | - | chr3.2:9712687-9712706 | MS.gene65850:intron | 55.0% | |
| !! | ATAGGCGTCGAGGAGAGTGT+TGG | - | chr3.2:9712017-9712036 | MS.gene65850:intron | 55.0% |
| CTGTGCTGTGCTGCCATAGC+AGG | - | chr3.2:9711934-9711953 | MS.gene65850:intron | 60.0% | |
| GGTCGCATGACGCACATGCT+AGG | - | chr3.2:9712686-9712705 | MS.gene65850:intron | 60.0% | |
| ! | TTCGCCGCAAATAGGCGTCG+AGG | - | chr3.2:9712007-9712026 | MS.gene65850:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 9711530 | 9713180 | 9711530 | ID=MS.gene65850 |
| chr3.2 | mRNA | 9711530 | 9713180 | 9711530 | ID=MS.gene65850.t1;Parent=MS.gene65850 |
| chr3.2 | exon | 9712965 | 9713180 | 9712965 | ID=MS.gene65850.t1.exon1;Parent=MS.gene65850.t1 |
| chr3.2 | CDS | 9712965 | 9713180 | 9712965 | ID=cds.MS.gene65850.t1;Parent=MS.gene65850.t1 |
| chr3.2 | exon | 9711530 | 9711619 | 9711530 | ID=MS.gene65850.t1.exon2;Parent=MS.gene65850.t1 |
| chr3.2 | CDS | 9711530 | 9711619 | 9711530 | ID=cds.MS.gene65850.t1;Parent=MS.gene65850.t1 |
| Gene Sequence |
| Protein sequence |
