AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene019036


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene019036.t1 MTR_3g045440 97.026 269 8 0 1 269 1 269 0.0 543
MS.gene019036.t1 MTR_6g086560 63.498 263 95 1 8 269 17 279 2.31e-125 358
MS.gene019036.t1 MTR_1g018320 38.077 260 159 2 7 265 5 263 3.78e-73 225
MS.gene019036.t1 MTR_4g095310 40.816 245 143 2 13 256 9 252 1.42e-70 218
MS.gene019036.t1 MTR_5g016150 38.776 245 148 2 13 256 9 252 7.29e-68 213
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene019036.t1 AT3G24420 50.185 271 132 2 1 268 1 271 3.00e-104 304
MS.gene019036.t1 AT3G03990 38.697 261 159 1 5 265 3 262 8.24e-76 231
MS.gene019036.t1 AT4G37470 42.041 245 140 2 13 256 9 252 1.99e-71 221

Find 53 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTAATGCCCTATGCTTAT+TGG 0.241237 3.2:-32292471 None:intergenic
TGACATAAAACTATTGTTTC+GGG 0.281340 3.2:-32291215 None:intergenic
TACTTGGATGAGAACATTAA+AGG 0.285814 3.2:+32292489 MS.gene019036:CDS
ATGACATAAAACTATTGTTT+CGG 0.322926 3.2:-32291216 None:intergenic
TTGGAACAGACCAGTCTATA+TGG 0.354404 3.2:+32291243 MS.gene019036:CDS
TCAATAGAGTCAGCATCATT+TGG 0.360021 3.2:-32292303 None:intergenic
ACAGTCTAGTTCTGTTTGAT+TGG 0.364702 3.2:+32291297 MS.gene019036:CDS
TGATGAGTTAATCACTCTTA+TGG 0.373825 3.2:+32291391 MS.gene019036:CDS
TGAGGTGCTGAAGGGTATTG+TGG 0.390938 3.2:+32292581 MS.gene019036:CDS
AATAAGCATAGGGCATTACT+TGG 0.397845 3.2:+32292473 MS.gene019036:CDS
GCTTTCGTATTGAGACTCTA+TGG 0.399369 3.2:-32292247 None:intergenic
TCTGTAATAGCTCCAGAAAA+TGG 0.417553 3.2:-32291320 None:intergenic
TCATTTGGATCCACTGCAAT+AGG 0.424577 3.2:-32292288 None:intergenic
AAAAGCCTGAGCTCTTTAAG+AGG 0.425563 3.2:+32291489 MS.gene019036:CDS
TAGAGTCTCAATACGAAAGC+TGG 0.436550 3.2:+32292250 MS.gene019036:CDS
CTCATCAGCATAAGGTTCAT+AGG 0.436848 3.2:-32291375 None:intergenic
ACTTGGATGAGAACATTAAA+GGG 0.437938 3.2:+32292490 MS.gene019036:CDS
CTATAGAGATATAGTTGAAA+AGG 0.445888 3.2:+32292401 MS.gene019036:CDS
CATTAAAGGGATGTCTACTT+TGG 0.446680 3.2:+32292503 MS.gene019036:CDS
ATAAATACAGATGACTATAA+AGG 0.458026 3.2:+32292186 MS.gene019036:CDS
ACAATCCTCTTAAAGAGCTC+AGG 0.466269 3.2:-32291494 None:intergenic
TGTTTCGGGTCCTGAGCCTA+TGG 0.470244 3.2:-32291201 None:intergenic
CACTCTATGTCTGCTATGAT+AGG 0.473029 3.2:+32291449 MS.gene019036:CDS
TTCTTCTTTGTGCTTCCCCA+AGG 0.483467 3.2:+32291516 MS.gene019036:CDS
GTGATTAACTCATCAGCATA+AGG 0.495421 3.2:-32291383 None:intergenic
AATCACTCTTATGGATGAGT+TGG 0.497837 3.2:+32291400 MS.gene019036:CDS
CAACAAAAGTGATACATTTG+AGG 0.498332 3.2:-32291424 None:intergenic
CTCAGTTAACTGCACACCTA+AGG 0.500170 3.2:+32292553 MS.gene019036:CDS
GAGGTGCTGAAGGGTATTGT+GGG 0.500980 3.2:+32292582 MS.gene019036:CDS
GTGCAGTTAACTGAGGGAAA+TGG 0.509673 3.2:-32292545 None:intergenic
TCAGCACCTCAACCAACCTT+AGG 0.510018 3.2:-32292569 None:intergenic
TTGGAGTTTATAGATATGAC+TGG 0.515619 3.2:+32292522 MS.gene019036:CDS
AAGGTTGGTTGAGGTGCTGA+AGG 0.529671 3.2:+32292572 MS.gene019036:CDS
AATGCAAGAACCATAGGCTC+AGG 0.553052 3.2:+32291191 MS.gene019036:CDS
GAAAGCTGGGTTTCAGCATA+CGG 0.556562 3.2:+32292264 MS.gene019036:CDS
TCTTTAAATGCAAGAACCAT+AGG 0.556752 3.2:+32291185 MS.gene019036:CDS
GTGGCAGTTCCAATAAGCAT+AGG 0.560917 3.2:+32292462 MS.gene019036:CDS
ACTTGACTGAATGATTGTGC+AGG 0.563263 3.2:-32292433 None:intergenic
AGCATACGGTCCTATTGCAG+TGG 0.564652 3.2:+32292278 MS.gene019036:CDS
TTGCATTTAAAGATGTTGAG+AGG 0.576785 3.2:-32291175 None:intergenic
AGAGTCTCAATACGAAAGCT+GGG 0.578249 3.2:+32292251 MS.gene019036:CDS
TGCACACCTAAGGTTGGTTG+AGG 0.583937 3.2:+32292563 MS.gene019036:CDS
TGGCAGTTCCAATAAGCATA+GGG 0.586920 3.2:+32292463 MS.gene019036:CDS
GTTAACTGCACACCTAAGGT+TGG 0.590880 3.2:+32292557 MS.gene019036:CDS
CTTGACTGAATGATTGTGCA+GGG 0.604202 3.2:-32292432 None:intergenic
AATACAGATGACTATAAAGG+AGG 0.635005 3.2:+32292189 MS.gene019036:CDS
CATTCAGTCAAGTAATGATG+TGG 0.635222 3.2:+32292443 MS.gene019036:CDS
TGGTGAAGTTGCAATTACCT+TGG 0.640152 3.2:+32292356 MS.gene019036:CDS
CTTAGGTGTGCAGTTAACTG+AGG 0.642434 3.2:-32292552 None:intergenic
AGGTTGGTTGAGGTGCTGAA+GGG 0.645453 3.2:+32292573 MS.gene019036:CDS
TTAGGTGTGCAGTTAACTGA+GGG 0.650507 3.2:-32292551 None:intergenic
AAATGCTTGAAAAGAATGGG+TGG 0.686067 3.2:+32292336 MS.gene019036:CDS
TTGACTGAATGATTGTGCAG+GGG 0.705699 3.2:-32292431 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAACTTTTTAACTATGTTAT+TGG + chr3.2:32291891-32291910 MS.gene019036:intron 15.0%
!!! TATATTTTGCATGATTATAA+AGG - chr3.2:32291352-32291371 None:intergenic 15.0%
!! AATTAGTTATTGACAAAACT+CGG - chr3.2:32291930-32291949 None:intergenic 20.0%
!! ACCTATGAATGAATAATTAT+GGG - chr3.2:32292164-32292183 None:intergenic 20.0%
!! ATAAATACAGATGACTATAA+AGG + chr3.2:32292186-32292205 MS.gene019036:CDS 20.0%
!! ATGACATAAAACTATTGTTT+CGG - chr3.2:32291219-32291238 None:intergenic 20.0%
!! TACCTATGAATGAATAATTA+TGG - chr3.2:32292165-32292184 None:intergenic 20.0%
!!! AAACAATAGTTTTATGTCAT+GGG + chr3.2:32291219-32291238 MS.gene019036:CDS 20.0%
! ATTAGTTATTGACAAAACTC+GGG - chr3.2:32291929-32291948 None:intergenic 25.0%
! CAAAAATGCTTGAAAAGAAT+GGG + chr3.2:32292333-32292352 MS.gene019036:CDS 25.0%
! CTATAGAGATATAGTTGAAA+AGG + chr3.2:32292401-32292420 MS.gene019036:CDS 25.0%
! GAAATCTCATATTGTGTATT+TGG + chr3.2:32291664-32291683 MS.gene019036:intron 25.0%
! TCAAAAATGCTTGAAAAGAA+TGG + chr3.2:32292332-32292351 MS.gene019036:CDS 25.0%
! TCCCATAATTATTCATTCAT+AGG + chr3.2:32292160-32292179 MS.gene019036:intron 25.0%
! TGACATAAAACTATTGTTTC+GGG - chr3.2:32291218-32291237 None:intergenic 25.0%
! TGTTAAAACGCTTGATTATT+TGG + chr3.2:32291640-32291659 MS.gene019036:intron 25.0%
!! AAACATTTGATGGTACAATA+TGG + chr3.2:32292110-32292129 MS.gene019036:intron 25.0%
!! AACATTTGATGGTACAATAT+GGG + chr3.2:32292111-32292130 MS.gene019036:intron 25.0%
!! CAATTTTATTCCATATAGAC+TGG - chr3.2:32291256-32291275 None:intergenic 25.0%
!! CTGTAATTTTCAGTTAAAAG+AGG - chr3.2:32291281-32291300 None:intergenic 25.0%
!!! GAAACAATAGTTTTATGTCA+TGG + chr3.2:32291218-32291237 MS.gene019036:CDS 25.0%
AATACAGATGACTATAAAGG+AGG + chr3.2:32292189-32292208 MS.gene019036:CDS 30.0%
ACTTGGATGAGAACATTAAA+GGG + chr3.2:32292490-32292509 MS.gene019036:CDS 30.0%
CAACAAAAGTGATACATTTG+AGG - chr3.2:32291427-32291446 None:intergenic 30.0%
GTACCATCAAATGTTTGAAT+TGG - chr3.2:32292106-32292125 None:intergenic 30.0%
TACTTGGATGAGAACATTAA+AGG + chr3.2:32292489-32292508 MS.gene019036:CDS 30.0%
TCTTTAAATGCAAGAACCAT+AGG + chr3.2:32291185-32291204 MS.gene019036:CDS 30.0%
TGATGAGTTAATCACTCTTA+TGG + chr3.2:32291391-32291410 MS.gene019036:CDS 30.0%
TTAGTTATTGACAAAACTCG+GGG - chr3.2:32291928-32291947 None:intergenic 30.0%
TTGCATTTAAAGATGTTGAG+AGG - chr3.2:32291178-32291197 None:intergenic 30.0%
TTGGAGTTTATAGATATGAC+TGG + chr3.2:32292522-32292541 MS.gene019036:CDS 30.0%
! TTTTGTGGAAGGACAAAAAT+AGG - chr3.2:32292037-32292056 None:intergenic 30.0%
!! CTCAAATGTATCACTTTTGT+TGG + chr3.2:32291425-32291444 MS.gene019036:CDS 30.0%
!!! ATAGTTTTATGTCATGGGTT+TGG + chr3.2:32291224-32291243 MS.gene019036:CDS 30.0%
!!! GTTTAGCATTTCTAGTTTTG+TGG - chr3.2:32292052-32292071 None:intergenic 30.0%
AAATGCTTGAAAAGAATGGG+TGG + chr3.2:32292336-32292355 MS.gene019036:CDS 35.0%
AAGTAATGCCCTATGCTTAT+TGG - chr3.2:32292474-32292493 None:intergenic 35.0%
AATCACTCTTATGGATGAGT+TGG + chr3.2:32291400-32291419 MS.gene019036:CDS 35.0%
AGGCCAATTCAAACATTTGA+TGG + chr3.2:32292100-32292119 MS.gene019036:intron 35.0%
CATTAAAGGGATGTCTACTT+TGG + chr3.2:32292503-32292522 MS.gene019036:CDS 35.0%
CATTCAGTCAAGTAATGATG+TGG + chr3.2:32292443-32292462 MS.gene019036:CDS 35.0%
GAAAAACATAGATGTGAGAG+AGG - chr3.2:32291989-32292008 None:intergenic 35.0%
GTGATTAACTCATCAGCATA+AGG - chr3.2:32291386-32291405 None:intergenic 35.0%
TAGTTATTGACAAAACTCGG+GGG - chr3.2:32291927-32291946 None:intergenic 35.0%
TCAATAGAGTCAGCATCATT+TGG - chr3.2:32292306-32292325 None:intergenic 35.0%
TCTGTAATAGCTCCAGAAAA+TGG - chr3.2:32291323-32291342 None:intergenic 35.0%
! AAAGAGCTCAGGCTTTTTAA+TGG - chr3.2:32291486-32291505 None:intergenic 35.0%
! AATAAGCATAGGGCATTACT+TGG + chr3.2:32292473-32292492 MS.gene019036:CDS 35.0%
! ACATCAGAGAGAGTATATGT+AGG - chr3.2:32291556-32291575 None:intergenic 35.0%
! ATTTGATGGTACAATATGGG+TGG + chr3.2:32292114-32292133 MS.gene019036:intron 35.0%
! GTCACTGAAAAACACAGTTT+TGG - chr3.2:32292382-32292401 None:intergenic 35.0%
! TCAAATGTTTGAATTGGCCT+AGG - chr3.2:32292100-32292119 None:intergenic 35.0%
!! ACAGTCTAGTTCTGTTTGAT+TGG + chr3.2:32291297-32291316 MS.gene019036:CDS 35.0%
!!! AGCATTTCTAGTTTTGTGGA+AGG - chr3.2:32292048-32292067 None:intergenic 35.0%
!!! TCACTTTTTTTTCCTAGCCT+AGG + chr3.2:32292080-32292099 MS.gene019036:intron 35.0%
AAAAGCCTGAGCTCTTTAAG+AGG + chr3.2:32291489-32291508 MS.gene019036:CDS 40.0%
ACAATCCTCTTAAAGAGCTC+AGG - chr3.2:32291497-32291516 None:intergenic 40.0%
ACTTGACTGAATGATTGTGC+AGG - chr3.2:32292436-32292455 None:intergenic 40.0%
AGAGTCTCAATACGAAAGCT+GGG + chr3.2:32292251-32292270 MS.gene019036:CDS 40.0%
CACTCTATGTCTGCTATGAT+AGG + chr3.2:32291449-32291468 MS.gene019036:CDS 40.0%
CATGTGTTACCGACATATGT+CGG + chr3.2:32291795-32291814 MS.gene019036:intron 40.0%
CTCATCAGCATAAGGTTCAT+AGG - chr3.2:32291378-32291397 None:intergenic 40.0%
CTTGACTGAATGATTGTGCA+GGG - chr3.2:32292435-32292454 None:intergenic 40.0%
GCTTTCGTATTGAGACTCTA+TGG - chr3.2:32292250-32292269 None:intergenic 40.0%
GTGCACTTCTTTATGTGTTG+TGG - chr3.2:32292015-32292034 None:intergenic 40.0%
TAGAGTCTCAATACGAAAGC+TGG + chr3.2:32292250-32292269 MS.gene019036:CDS 40.0%
TAGTGTCAACGTGTTAGTGA+CGG - chr3.2:32291732-32291751 None:intergenic 40.0%
TCATTTGGATCCACTGCAAT+AGG - chr3.2:32292291-32292310 None:intergenic 40.0%
TGGCAGTTCCAATAAGCATA+GGG + chr3.2:32292463-32292482 MS.gene019036:CDS 40.0%
TGGTGAAGTTGCAATTACCT+TGG + chr3.2:32292356-32292375 MS.gene019036:CDS 40.0%
TTAGGTGTGCAGTTAACTGA+GGG - chr3.2:32292554-32292573 None:intergenic 40.0%
TTGACTGAATGATTGTGCAG+GGG - chr3.2:32292434-32292453 None:intergenic 40.0%
TTGGAACAGACCAGTCTATA+TGG + chr3.2:32291243-32291262 MS.gene019036:CDS 40.0%
! GAGAGAGGCTACTTTAAAGA+TGG - chr3.2:32291974-32291993 None:intergenic 40.0%
!! AGGTAAAGATGAGAGTACCT+TGG - chr3.2:32291536-32291555 None:intergenic 40.0%
!! CTGTTTGATTGGCCATTTTC+TGG + chr3.2:32291308-32291327 MS.gene019036:CDS 40.0%
!! GAAAAACACAGTTTTGGCCA+AGG - chr3.2:32292376-32292395 None:intergenic 40.0%
!! GGTAAAGATGAGAGTACCTT+GGG - chr3.2:32291535-32291554 None:intergenic 40.0%
!! GTAAAGATGAGAGTACCTTG+GGG - chr3.2:32291534-32291553 None:intergenic 40.0%
!!! GATGTTGAGAGGCTCTTTTT+TGG - chr3.2:32291167-32291186 None:intergenic 40.0%
AATGCAAGAACCATAGGCTC+AGG + chr3.2:32291191-32291210 MS.gene019036:CDS 45.0%
CTCAGTTAACTGCACACCTA+AGG + chr3.2:32292553-32292572 MS.gene019036:CDS 45.0%
CTTAGGTGTGCAGTTAACTG+AGG - chr3.2:32292555-32292574 None:intergenic 45.0%
GAAAGCTGGGTTTCAGCATA+CGG + chr3.2:32292264-32292283 MS.gene019036:CDS 45.0%
GTCTGATGTCCGACATATGT+CGG - chr3.2:32291807-32291826 None:intergenic 45.0%
GTGCAGTTAACTGAGGGAAA+TGG - chr3.2:32292548-32292567 None:intergenic 45.0%
GTGGCAGTTCCAATAAGCAT+AGG + chr3.2:32292462-32292481 MS.gene019036:CDS 45.0%
GTTAACTGCACACCTAAGGT+TGG + chr3.2:32292557-32292576 MS.gene019036:CDS 45.0%
TTCTTCTTTGTGCTTCCCCA+AGG + chr3.2:32291516-32291535 MS.gene019036:CDS 45.0%
! ACGCTTTCCATCAGAAGTGT+CGG + chr3.2:32291827-32291846 MS.gene019036:intron 45.0%
! GAGCTCAGGCTTTTTAATGG+AGG - chr3.2:32291483-32291502 None:intergenic 45.0%
! TGTTTGAATTGGCCTAGGCT+AGG - chr3.2:32292095-32292114 None:intergenic 45.0%
!! GTGTCGGTGCTACAAAGATT+TGG + chr3.2:32291843-32291862 MS.gene019036:intron 45.0%
AGCATACGGTCCTATTGCAG+TGG + chr3.2:32292278-32292297 MS.gene019036:CDS 50.0%
TCAGCACCTCAACCAACCTT+AGG - chr3.2:32292572-32292591 None:intergenic 50.0%
! TGCACACCTAAGGTTGGTTG+AGG + chr3.2:32292563-32292582 MS.gene019036:CDS 50.0%
!! AAGGTTGGTTGAGGTGCTGA+AGG + chr3.2:32292572-32292591 MS.gene019036:CDS 50.0%
!! AGGTTGGTTGAGGTGCTGAA+GGG + chr3.2:32292573-32292592 MS.gene019036:CDS 50.0%
!! GAGGTGCTGAAGGGTATTGT+GGG + chr3.2:32292582-32292601 MS.gene019036:CDS 50.0%
!! TGAGGTGCTGAAGGGTATTG+TGG + chr3.2:32292581-32292600 MS.gene019036:CDS 50.0%
!! TGTAGCACCGACACTTCTGA+TGG - chr3.2:32291837-32291856 None:intergenic 50.0%
! TGTTTCGGGTCCTGAGCCTA+TGG - chr3.2:32291204-32291223 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 32291155 32292608 32291155 ID=MS.gene019036
chr3.2 mRNA 32291155 32292608 32291155 ID=MS.gene019036.t1;Parent=MS.gene019036
chr3.2 exon 32291155 32291537 32291155 ID=MS.gene019036.t1.exon1;Parent=MS.gene019036.t1
chr3.2 CDS 32291155 32291537 32291155 ID=cds.MS.gene019036.t1;Parent=MS.gene019036.t1
chr3.2 exon 32292182 32292608 32292182 ID=MS.gene019036.t1.exon2;Parent=MS.gene019036.t1
chr3.2 CDS 32292182 32292608 32292182 ID=cds.MS.gene019036.t1;Parent=MS.gene019036.t1
Gene Sequence

>MS.gene019036

ATGGTGACACCAAAAAAGAGCCTCTCAACATCTTTAAATGCAAGAACCATAGGCTCAGGACCCGAAACAATAGTTTTATGTCATGGGTTTGGAACAGACCAGTCTATATGGAATAAAATTGTTCCTCTTTTAACTGAAAATTACAGTCTAGTTCTGTTTGATTGGCCATTTTCTGGAGCTATTACAGATAAAAGCCTTTATAATCATGCAAAATATAATTCCTATGAACCTTATGCTGATGAGTTAATCACTCTTATGGATGAGTTGGACCTCAAATGTATCACTTTTGTTGGACACTCTATGTCTGCTATGATAGGTTGCATTGCCTCCATTAAAAAGCCTGAGCTCTTTAAGAGGATTGTTCTTCTTTGTGCTTCCCCAAGGTACTCTCATCTTTACCTACATATACTCTCTCTGATGTTGTCTTTTTTTTTTATTCTCTTTTTATGAAAATACTAGAAGTAACACAATATTTGAGATTCTGTTGTTAAAACGCTTGATTATTTGGTGAAATCTCATATTGTGTATTTGGATCTATGAAGTATCGTCACAAACACTAATATCATATAAGACACCGTCACTAACACGTTGACACTAGTAAAAATATGAAGAAAAAAAATGAATTAATTGAACGTAATCACATGTGTTACCGACATATGTCGGACATCAGACACGCTTTCCATCAGAAGTGTCGGTGCTACAAAGATTTGGATAAATATTTGAAAGTAATTTAAAGTAACTTTTTAACTATGTTATTGGTGCAAAGTCTCCCCCGAGTTTTGTCAATAACTAATTTCTGTCGAGAAAATTCACTAACCATCTTTAAAGTAGCCTCTCTCACATCTATGTTTTTCTATCCACAACACATAAAGAAGTGCACCTATTTTTGTCCTTCCACAAAACTAGAAATGCTAAACTCGATAAATCACTTTTTTTTCCTAGCCTAGGCCAATTCAAACATTTGATGGTACAATATGGGTGGAATCAAATAGTCTTGCATTAACATCCCATAATTATTCATTCATAGGTATATAAATACAGATGACTATAAAGGAGGCTTTGAGAGATCAGATATTGAGAACTTAATATCAACCATAGAGTCTCAATACGAAAGCTGGGTTTCAGCATACGGTCCTATTGCAGTGGATCCAAATGATGCTGACTCTATTGACAAATTTCAAAAATGCTTGAAAAGAATGGGTGGTGAAGTTGCAATTACCTTGGCCAAAACTGTGTTTTTCAGTGACTATAGAGATATAGTTGAAAAGGTAGTGATCCCCTGCACAATCATTCAGTCAAGTAATGATGTGGCAGTTCCAATAAGCATAGGGCATTACTTGGATGAGAACATTAAAGGGATGTCTACTTTGGAGTTTATAGATATGACTGGCCATTTCCCTCAGTTAACTGCACACCTAAGGTTGGTTGAGGTGCTGAAGGGTATTGTGGGATAG

Protein sequence

>MS.gene019036.t1

MVTPKKSLSTSLNARTIGSGPETIVLCHGFGTDQSIWNKIVPLLTENYSLVLFDWPFSGAITDKSLYNHAKYNSYEPYADELITLMDELDLKCITFVGHSMSAMIGCIASIKKPELFKRIVLLCASPRYINTDDYKGGFERSDIENLISTIESQYESWVSAYGPIAVDPNDADSIDKFQKCLKRMGGEVAITLAKTVFFSDYRDIVEKVVIPCTIIQSSNDVAVPISIGHYLDENIKGMSTLEFIDMTGHFPQLTAHLRLVEVLKGIVG