Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041911.t1 | XP_003608457.1 | 99.6 | 270 | 1 | 0 | 1 | 270 | 1 | 270 | 1.80E-149 | 538.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041911.t1 | Q9SZU7 | 78.8 | 269 | 57 | 0 | 1 | 269 | 1 | 269 | 2.0e-125 | 449.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041911.t1 | G7JCS9 | 99.6 | 270 | 1 | 0 | 1 | 270 | 1 | 270 | 1.3e-149 | 538.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene24472 | MS.gene041911 | PPI |
| MS.gene00497 | MS.gene041911 | PPI |
| MS.gene03694 | MS.gene041911 | PPI |
| MS.gene89421 | MS.gene041911 | PPI |
| MS.gene019036 | MS.gene041911 | PPI |
| MS.gene041911 | MS.gene66455 | PPI |
| MS.gene29395 | MS.gene041911 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041911.t1 | MTR_4g095310 | 99.630 | 270 | 1 | 0 | 1 | 270 | 1 | 270 | 0.0 | 555 |
| MS.gene041911.t1 | MTR_5g016150 | 79.851 | 268 | 54 | 0 | 1 | 268 | 1 | 268 | 4.96e-169 | 469 |
| MS.gene041911.t1 | MTR_1g018320 | 57.430 | 249 | 104 | 2 | 6 | 253 | 8 | 255 | 4.06e-107 | 311 |
| MS.gene041911.t1 | MTR_3g045440 | 40.408 | 245 | 144 | 2 | 9 | 252 | 13 | 256 | 7.99e-69 | 214 |
| MS.gene041911.t1 | MTR_6g086560 | 40.157 | 254 | 149 | 3 | 9 | 260 | 22 | 274 | 3.86e-66 | 207 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041911.t1 | AT4G37470 | 78.810 | 269 | 57 | 0 | 1 | 269 | 1 | 269 | 9.02e-166 | 460 |
| MS.gene041911.t1 | AT3G03990 | 57.661 | 248 | 104 | 1 | 6 | 253 | 8 | 254 | 1.03e-107 | 313 |
| MS.gene041911.t1 | AT3G24420 | 43.548 | 248 | 137 | 3 | 7 | 252 | 13 | 259 | 4.92e-76 | 233 |
Find 55 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGATTTGAGCAAGAAGATT+TGG | 0.309415 | 8.4:-25482588 | MS.gene041911:CDS |
| CTCGCCGGACGTTGTTATTC+CGG | 0.318614 | 8.4:-25482231 | MS.gene041911:CDS |
| CGCTCAAAATCAAAGTAATC+TGG | 0.326618 | 8.4:+25483678 | None:intergenic |
| CATTCTGTTTCTGCTATGAT+TGG | 0.327046 | 8.4:-25483573 | MS.gene041911:CDS |
| GAGCGTTATTCAACCTTAGA+AGG | 0.329474 | 8.4:-25483660 | MS.gene041911:CDS |
| AATTACAAATCATGGTGTTC+TGG | 0.329664 | 8.4:-25482539 | MS.gene041911:CDS |
| GTTGATTCATGTATCTTTGT+TGG | 0.344178 | 8.4:-25483597 | MS.gene041911:CDS |
| AAATTGTTTGTAACACGCTT+AGG | 0.357437 | 8.4:+25482426 | None:intergenic |
| ATCATAAGCATAACCTTCTA+AGG | 0.361019 | 8.4:+25483647 | None:intergenic |
| TATGAAGGATCTAGCGGTTC+CGG | 0.394091 | 8.4:-25482342 | MS.gene041911:CDS |
| ATAGTTCTAGCTCACGGGTT+TGG | 0.396140 | 8.4:-25483795 | MS.gene041911:CDS |
| AATTGTTTGTAACACGCTTA+GGG | 0.419608 | 8.4:+25482427 | None:intergenic |
| TGGCATCAAATTACAAATCA+TGG | 0.445032 | 8.4:-25482547 | MS.gene041911:CDS |
| AGCGGTTCCGGTCGTGGTCG+CGG | 0.451096 | 8.4:-25482330 | MS.gene041911:CDS |
| AACGACAGGGACTACTTTGG+AGG | 0.459003 | 8.4:-25482608 | MS.gene041911:CDS |
| GGATCTAGCGGTTCCGGTCG+TGG | 0.461437 | 8.4:-25482336 | MS.gene041911:CDS |
| GGATTTGCTCCAATGGCAGT+TGG | 0.462285 | 8.4:-25482518 | MS.gene041911:CDS |
| ATGTCCCCTCCAACTGCCAT+TGG | 0.469708 | 8.4:+25482509 | None:intergenic |
| GGATCAACTATTCGATGCTA+TGG | 0.479394 | 8.4:-25482567 | MS.gene041911:CDS |
| TTGAACGACAGGGACTACTT+TGG | 0.492694 | 8.4:-25482611 | MS.gene041911:CDS |
| GGGGAAGGTATTTGAACGAC+AGG | 0.494683 | 8.4:-25482622 | MS.gene041911:intron |
| TGCTCCAATGGCAGTTGGAG+GGG | 0.510556 | 8.4:-25482513 | MS.gene041911:CDS |
| CCTTCATACTTTGTATAATG+TGG | 0.518604 | 8.4:+25482357 | None:intergenic |
| TACTTTGTATAATGTGGCAT+GGG | 0.519899 | 8.4:+25482363 | None:intergenic |
| AATGGCAGTTGGAGGGGACA+TGG | 0.527300 | 8.4:-25482507 | MS.gene041911:CDS |
| AGATATTCCGCGACCACGAC+CGG | 0.529415 | 8.4:+25482323 | None:intergenic |
| GTGTTCTGGATTTGCTCCAA+TGG | 0.532527 | 8.4:-25482525 | MS.gene041911:CDS |
| GGTTCATAGTTCTAGCTCAC+GGG | 0.534969 | 8.4:-25483800 | MS.gene041911:CDS |
| TTGGCACAGATCAATCTGTA+TGG | 0.535156 | 8.4:-25483776 | MS.gene041911:CDS |
| ATCACCGGAATAACAACGTC+CGG | 0.537857 | 8.4:+25482227 | None:intergenic |
| CCACATTATACAAAGTATGA+AGG | 0.538729 | 8.4:-25482357 | MS.gene041911:CDS |
| TTTGCTCCAATGGCAGTTGG+AGG | 0.538790 | 8.4:-25482515 | MS.gene041911:CDS |
| ATACTTTGTATAATGTGGCA+TGG | 0.539342 | 8.4:+25482362 | None:intergenic |
| CTTTATGACAACATGGGAGC+TGG | 0.543266 | 8.4:-25483711 | MS.gene041911:CDS |
| TTGCTCCAATGGCAGTTGGA+GGG | 0.545271 | 8.4:-25482514 | MS.gene041911:CDS |
| CGAATGTGTTTGAGTATCAC+CGG | 0.546328 | 8.4:+25482212 | None:intergenic |
| CGGTTCATAGTTCTAGCTCA+CGG | 0.558590 | 8.4:-25483801 | MS.gene041911:CDS |
| AACACGCTTAGGGCTATGTC+TGG | 0.562881 | 8.4:+25482437 | None:intergenic |
| ACTCGCTACCAACATGTTGG+TGG | 0.576429 | 8.4:+25482300 | None:intergenic |
| GTGGAGGTTACGTCGACAGA+AGG | 0.584512 | 8.4:-25482272 | MS.gene041911:CDS |
| ACACATTCGTCATGATATTG+CGG | 0.602861 | 8.4:-25482198 | MS.gene041911:CDS |
| GAATATCTCCACCAACATGT+TGG | 0.605480 | 8.4:-25482308 | MS.gene041911:CDS |
| TGGACTCGCTACCAACATGT+TGG | 0.613943 | 8.4:+25482297 | None:intergenic |
| CATGAGACAAATATTATGCA+TGG | 0.615267 | 8.4:-25482393 | MS.gene041911:CDS |
| TTTGCCGCAATTAAGCTCGC+CGG | 0.620386 | 8.4:-25482246 | MS.gene041911:CDS |
| TGTCATACTTTATGACAACA+TGG | 0.621368 | 8.4:-25483718 | MS.gene041911:CDS |
| ACGTCCGGCGAGCTTAATTG+CGG | 0.647186 | 8.4:+25482242 | None:intergenic |
| CGAAACTCATCTAAGAGATG+AGG | 0.649391 | 8.4:+25483741 | None:intergenic |
| ACAAAGTATGAAGGATCTAG+CGG | 0.659829 | 8.4:-25482348 | MS.gene041911:CDS |
| GGGAAGGTATTTGAACGACA+GGG | 0.664911 | 8.4:-25482621 | MS.gene041911:intron |
| GTCATACTTTATGACAACAT+GGG | 0.668133 | 8.4:-25483717 | MS.gene041911:CDS |
| CATCTAAGAGATGAGGTACG+AGG | 0.688542 | 8.4:+25483748 | None:intergenic |
| TGTTGGTAGCGAGTCCATCG+TGG | 0.709388 | 8.4:-25482291 | MS.gene041911:CDS |
| TGGTAGCGAGTCCATCGTGG+AGG | 0.724029 | 8.4:-25482288 | MS.gene041911:CDS |
| TGTCGACGTAACCTCCACGA+TGG | 0.724490 | 8.4:+25482277 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATTAGTCCAATATTTA+TGG | + | chr8.4:25482745-25482764 | None:intergenic | 15.0% |
| !! | ATTATTAATGATGTAAATGA+TGG | - | chr8.4:25483392-25483411 | MS.gene041911:intron | 15.0% |
| !! | TAATGTATCAATAGTTAATA+AGG | - | chr8.4:25483160-25483179 | MS.gene041911:intron | 15.0% |
| !! | TTATTAATGATGTAAATGAT+GGG | - | chr8.4:25483393-25483412 | MS.gene041911:intron | 15.0% |
| !!! | TAAATATCATATATTTTGAG+TGG | - | chr8.4:25482973-25482992 | MS.gene041911:intron | 15.0% |
| !!! | TTTTGATCATTATATTATGT+AGG | - | chr8.4:25483035-25483054 | MS.gene041911:intron | 15.0% |
| !! | ATTATATTATGTAGGCATAT+TGG | - | chr8.4:25483043-25483062 | MS.gene041911:intron | 20.0% |
| !! | GATAAAAATTATCTCTTGAA+AGG | + | chr8.4:25482889-25482908 | None:intergenic | 20.0% |
| !! | GATCAAAATAAAAACTACAT+AGG | + | chr8.4:25483023-25483042 | None:intergenic | 20.0% |
| !! | TATTAATGATGTAAATGATG+GGG | - | chr8.4:25483394-25483413 | MS.gene041911:intron | 20.0% |
| !!! | ATAATTTTGTTGAATAGATC+AGG | + | chr8.4:25482520-25482539 | None:intergenic | 20.0% |
| !!! | TTTTTTCTTTCTTTCATCTT+TGG | - | chr8.4:25483223-25483242 | MS.gene041911:intron | 20.0% |
| !!! | TTTTTTGTCTCCATAAATAT+TGG | - | chr8.4:25482732-25482751 | MS.gene041911:intron | 20.0% |
| ! | ATTAATGATGTAAATGATGG+GGG | - | chr8.4:25483395-25483414 | MS.gene041911:intron | 25.0% |
| !! | TCAACAAAGTCTTATATTTG+AGG | + | chr8.4:25482835-25482854 | None:intergenic | 25.0% |
| !!! | AATTTAATTTGACCATACCT+TGG | + | chr8.4:25482559-25482578 | None:intergenic | 25.0% |
| !!! | TAGTGCATCCTTTTTTTTAA+AGG | + | chr8.4:25483191-25483210 | None:intergenic | 25.0% |
| AAATTGTTTGTAACACGCTT+AGG | + | chr8.4:25483622-25483641 | None:intergenic | 30.0% | |
| AATTACAAATCATGGTGTTC+TGG | - | chr8.4:25483506-25483525 | MS.gene041911:intron | 30.0% | |
| AATTGTTTGTAACACGCTTA+GGG | + | chr8.4:25483621-25483640 | None:intergenic | 30.0% | |
| ATACTTTGTATAATGTGGCA+TGG | + | chr8.4:25483686-25483705 | None:intergenic | 30.0% | |
| ATCATAAGCATAACCTTCTA+AGG | + | chr8.4:25482401-25482420 | None:intergenic | 30.0% | |
| CATGAGACAAATATTATGCA+TGG | - | chr8.4:25483652-25483671 | MS.gene041911:CDS | 30.0% | |
| CCACATTATACAAAGTATGA+AGG | - | chr8.4:25483688-25483707 | MS.gene041911:CDS | 30.0% | |
| CCTTCATACTTTGTATAATG+TGG | + | chr8.4:25483691-25483710 | None:intergenic | 30.0% | |
| GTCATACTTTATGACAACAT+GGG | - | chr8.4:25482328-25482347 | MS.gene041911:CDS | 30.0% | |
| TACTTTGTATAATGTGGCAT+GGG | + | chr8.4:25483685-25483704 | None:intergenic | 30.0% | |
| TGGCATCAAATTACAAATCA+TGG | - | chr8.4:25483498-25483517 | MS.gene041911:intron | 30.0% | |
| TGTCATACTTTATGACAACA+TGG | - | chr8.4:25482327-25482346 | MS.gene041911:CDS | 30.0% | |
| ! | AGGATGCACCTTTAAAAAAA+AGG | - | chr8.4:25483180-25483199 | MS.gene041911:intron | 30.0% |
| ! | GTTGATTCATGTATCTTTGT+TGG | - | chr8.4:25482448-25482467 | MS.gene041911:CDS | 30.0% |
| ACAAAGTATGAAGGATCTAG+CGG | - | chr8.4:25483697-25483716 | MS.gene041911:CDS | 35.0% | |
| ACACATTCGTCATGATATTG+CGG | - | chr8.4:25483847-25483866 | MS.gene041911:CDS | 35.0% | |
| AGGATTTGAGCAAGAAGATT+TGG | - | chr8.4:25483457-25483476 | MS.gene041911:intron | 35.0% | |
| CGCTCAAAATCAAAGTAATC+TGG | + | chr8.4:25482370-25482389 | None:intergenic | 35.0% | |
| ! | ATGTAAATGATGGGGGAATT+TGG | - | chr8.4:25483402-25483421 | MS.gene041911:intron | 35.0% |
| ! | TGTAAATGATGGGGGAATTT+GGG | - | chr8.4:25483403-25483422 | MS.gene041911:intron | 35.0% |
| ! | TTTTGATATCAGCTTCTCCA+AGG | - | chr8.4:25482539-25482558 | MS.gene041911:CDS | 35.0% |
| !! | CATTCTGTTTCTGCTATGAT+TGG | - | chr8.4:25482472-25482491 | MS.gene041911:CDS | 35.0% |
| !!! | AACGTGAAAGTTTTAGGATC+AGG | - | chr8.4:25482217-25482236 | MS.gene041911:CDS | 35.0% |
| !!! | ACGTGAAAGTTTTAGGATCA+GGG | - | chr8.4:25482218-25482237 | MS.gene041911:CDS | 35.0% |
| ATATCAGCTTCTCCAAGGTA+TGG | - | chr8.4:25482544-25482563 | MS.gene041911:CDS | 40.0% | |
| CGAAACTCATCTAAGAGATG+AGG | + | chr8.4:25482307-25482326 | None:intergenic | 40.0% | |
| CGAATGTGTTTGAGTATCAC+CGG | + | chr8.4:25483836-25483855 | None:intergenic | 40.0% | |
| GAATATCTCCACCAACATGT+TGG | - | chr8.4:25483737-25483756 | MS.gene041911:CDS | 40.0% | |
| GAGCGTTATTCAACCTTAGA+AGG | - | chr8.4:25482385-25482404 | MS.gene041911:CDS | 40.0% | |
| GGATCAACTATTCGATGCTA+TGG | - | chr8.4:25483478-25483497 | MS.gene041911:intron | 40.0% | |
| TGAGTGTGTGTGTGTGTATA+TGG | - | chr8.4:25483304-25483323 | MS.gene041911:intron | 40.0% | |
| TTGGCACAGATCAATCTGTA+TGG | - | chr8.4:25482269-25482288 | MS.gene041911:CDS | 40.0% | |
| ! | GCTCACAACGTGAAAGTTTT+AGG | - | chr8.4:25482211-25482230 | MS.gene041911:CDS | 40.0% |
| ! | GTAAATGATGGGGGAATTTG+GGG | - | chr8.4:25483404-25483423 | MS.gene041911:intron | 40.0% |
| !!! | AAGTTTTAGGATCAGGGTCA+CGG | - | chr8.4:25482224-25482243 | MS.gene041911:CDS | 40.0% |
| ATCACCGGAATAACAACGTC+CGG | + | chr8.4:25483821-25483840 | None:intergenic | 45.0% | |
| CATCTAAGAGATGAGGTACG+AGG | + | chr8.4:25482300-25482319 | None:intergenic | 45.0% | |
| CTTTATGACAACATGGGAGC+TGG | - | chr8.4:25482334-25482353 | MS.gene041911:CDS | 45.0% | |
| TATGAAGGATCTAGCGGTTC+CGG | - | chr8.4:25483703-25483722 | MS.gene041911:CDS | 45.0% | |
| TTGAACGACAGGGACTACTT+TGG | - | chr8.4:25483434-25483453 | MS.gene041911:intron | 45.0% | |
| ! | GTGTTCTGGATTTGCTCCAA+TGG | - | chr8.4:25483520-25483539 | MS.gene041911:CDS | 45.0% |
| !! | ATAGTTCTAGCTCACGGGTT+TGG | - | chr8.4:25482250-25482269 | MS.gene041911:CDS | 45.0% |
| !! | CGGTTCATAGTTCTAGCTCA+CGG | - | chr8.4:25482244-25482263 | MS.gene041911:CDS | 45.0% |
| !! | GGGAAGGTATTTGAACGACA+GGG | - | chr8.4:25483424-25483443 | MS.gene041911:intron | 45.0% |
| !! | GGTTCATAGTTCTAGCTCAC+GGG | - | chr8.4:25482245-25482264 | MS.gene041911:CDS | 45.0% |
| !!! | AAATATTAATTTTATTCTTA+AGG | - | chr8.4:25483107-25483126 | MS.gene041911:intron | 5.0% |
| !!! | TAAATTATAATATTGATTTT+AGG | - | chr8.4:25482770-25482789 | MS.gene041911:intron | 5.0% |
| AACACGCTTAGGGCTATGTC+TGG | + | chr8.4:25483611-25483630 | None:intergenic | 50.0% | |
| AACGACAGGGACTACTTTGG+AGG | - | chr8.4:25483437-25483456 | MS.gene041911:intron | 50.0% | |
| ACTCGCTACCAACATGTTGG+TGG | + | chr8.4:25483748-25483767 | None:intergenic | 50.0% | |
| TGGACTCGCTACCAACATGT+TGG | + | chr8.4:25483751-25483770 | None:intergenic | 50.0% | |
| TTGCTCCAATGGCAGTTGGA+GGG | - | chr8.4:25483531-25483550 | MS.gene041911:CDS | 50.0% | |
| TTTGCCGCAATTAAGCTCGC+CGG | - | chr8.4:25483799-25483818 | MS.gene041911:CDS | 50.0% | |
| TTTGCTCCAATGGCAGTTGG+AGG | - | chr8.4:25483530-25483549 | MS.gene041911:CDS | 50.0% | |
| ! | ATGATGGGGGAATTTGGGGA+AGG | - | chr8.4:25483408-25483427 | MS.gene041911:intron | 50.0% |
| ! | GGATTTGCTCCAATGGCAGT+TGG | - | chr8.4:25483527-25483546 | MS.gene041911:CDS | 50.0% |
| !! | GGGGAAGGTATTTGAACGAC+AGG | - | chr8.4:25483423-25483442 | MS.gene041911:intron | 50.0% |
| AATGGCAGTTGGAGGGGACA+TGG | - | chr8.4:25483538-25483557 | MS.gene041911:CDS | 55.0% | |
| ACGTCCGGCGAGCTTAATTG+CGG | + | chr8.4:25483806-25483825 | None:intergenic | 55.0% | |
| AGATATTCCGCGACCACGAC+CGG | + | chr8.4:25483725-25483744 | None:intergenic | 55.0% | |
| ATGTCCCCTCCAACTGCCAT+TGG | + | chr8.4:25483539-25483558 | None:intergenic | 55.0% | |
| GTGGAGGTTACGTCGACAGA+AGG | - | chr8.4:25483773-25483792 | MS.gene041911:CDS | 55.0% | |
| TGCTCCAATGGCAGTTGGAG+GGG | - | chr8.4:25483532-25483551 | MS.gene041911:CDS | 55.0% | |
| TGTCGACGTAACCTCCACGA+TGG | + | chr8.4:25483771-25483790 | None:intergenic | 55.0% | |
| ! | CTCGCCGGACGTTGTTATTC+CGG | - | chr8.4:25483814-25483833 | MS.gene041911:CDS | 55.0% |
| !! | TGTTGGTAGCGAGTCCATCG+TGG | - | chr8.4:25483754-25483773 | MS.gene041911:CDS | 55.0% |
| ! | TGGTAGCGAGTCCATCGTGG+AGG | - | chr8.4:25483757-25483776 | MS.gene041911:CDS | 60.0% |
| GGATCTAGCGGTTCCGGTCG+TGG | - | chr8.4:25483709-25483728 | MS.gene041911:CDS | 65.0% | |
| AGCGGTTCCGGTCGTGGTCG+CGG | - | chr8.4:25483715-25483734 | MS.gene041911:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 25482193 | 25483874 | 25482193 | ID=MS.gene041911 |
| chr8.4 | mRNA | 25482193 | 25483874 | 25482193 | ID=MS.gene041911.t1;Parent=MS.gene041911 |
| chr8.4 | exon | 25483507 | 25483874 | 25483507 | ID=MS.gene041911.t1.exon1;Parent=MS.gene041911.t1 |
| chr8.4 | CDS | 25483507 | 25483874 | 25483507 | ID=cds.MS.gene041911.t1;Parent=MS.gene041911.t1 |
| chr8.4 | exon | 25482193 | 25482637 | 25482193 | ID=MS.gene041911.t1.exon2;Parent=MS.gene041911.t1 |
| chr8.4 | CDS | 25482193 | 25482637 | 25482193 | ID=cds.MS.gene041911.t1;Parent=MS.gene041911.t1 |
| Gene Sequence |
| Protein sequence |
