Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019286.t1 | AES94086.2 | 100 | 270 | 0 | 0 | 1 | 270 | 33 | 302 | 4.50E-145 | 523.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019286.t1 | Q54KJ8 | 25.6 | 223 | 150 | 6 | 10 | 218 | 6 | 226 | 3.0e-12 | 73.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019286.t1 | G7KDE9 | 100.0 | 270 | 0 | 0 | 1 | 270 | 1 | 270 | 3.3e-145 | 523.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene019286 | MS.gene82237 | PPI |
| MS.gene019286 | MS.gene48819 | PPI |
| MS.gene019286 | MS.gene42075 | PPI |
| MS.gene76728 | MS.gene019286 | PPI |
| MS.gene77045 | MS.gene019286 | PPI |
| MS.gene87743 | MS.gene019286 | PPI |
| MS.gene87064 | MS.gene019286 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019286.t1 | MTR_5g010620 | 100.000 | 270 | 0 | 0 | 1 | 270 | 33 | 302 | 0.0 | 556 |
| MS.gene019286.t1 | MTR_5g010620 | 100.000 | 270 | 0 | 0 | 1 | 270 | 1 | 270 | 0.0 | 555 |
| MS.gene019286.t1 | MTR_8g096950 | 75.445 | 281 | 55 | 2 | 1 | 267 | 1 | 281 | 5.76e-154 | 430 |
| MS.gene019286.t1 | MTR_7g055620 | 49.558 | 113 | 31 | 4 | 6 | 118 | 171 | 257 | 2.15e-19 | 87.0 |
| MS.gene019286.t1 | MTR_0411s0050 | 59.649 | 57 | 23 | 0 | 75 | 131 | 15 | 71 | 2.86e-19 | 80.5 |
| MS.gene019286.t1 | MTR_3g110015 | 60.000 | 80 | 18 | 2 | 6 | 71 | 6 | 85 | 3.20e-19 | 80.5 |
| MS.gene019286.t1 | MTR_3g109978 | 60.000 | 80 | 18 | 2 | 6 | 71 | 97 | 176 | 3.50e-18 | 80.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene019286.t1 | AT4G20330 | 64.912 | 285 | 82 | 4 | 1 | 270 | 1 | 282 | 9.51e-122 | 349 |
| MS.gene019286.t1 | AT4G21010 | 55.036 | 278 | 110 | 6 | 1 | 266 | 1 | 275 | 1.21e-93 | 277 |
Find 52 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTGCTGTTATTGACTTAA+AGG | 0.158931 | 5.4:-4856456 | MS.gene019286:CDS |
| CTTCTGGAAATTTGCGTATC+AGG | 0.239866 | 5.4:+4856480 | None:intergenic |
| TCTCCATCGTATCTTACTTT+TGG | 0.252078 | 5.4:+4856682 | None:intergenic |
| AACTGTAATGGAGGACTTAC+AGG | 0.272992 | 5.4:-4856423 | MS.gene019286:intron |
| ATGGGCACTTTAGGATCATT+AGG | 0.284072 | 5.4:+4852052 | None:intergenic |
| TCAATAACAGCAATGCCTTC+TGG | 0.315673 | 5.4:+4856464 | None:intergenic |
| AGGTCATCATCCACCTTAAT+GGG | 0.315953 | 5.4:+4852034 | None:intergenic |
| TGAAAGGTGGCAGGGAGATT+TGG | 0.323843 | 5.4:-4852114 | MS.gene019286:CDS |
| TAGGTCATCATCCACCTTAA+TGG | 0.327997 | 5.4:+4852033 | None:intergenic |
| TCCACCTTAATGGGCACTTT+AGG | 0.329618 | 5.4:+4852043 | None:intergenic |
| TGATCCTAAAGTGCCCATTA+AGG | 0.368344 | 5.4:-4852047 | MS.gene019286:CDS |
| TTGACCGGAGCAAGAGCATT+TGG | 0.376765 | 5.4:+4859454 | None:intergenic |
| CTCTTTGCAGTGTTAGTAGC+AGG | 0.378308 | 5.4:+4851932 | None:intergenic |
| CGCTTCATTTGAGCTCCAAC+AGG | 0.397843 | 5.4:+4859379 | None:intergenic |
| GCTGACTAATGCCCATCTTC+CGG | 0.401133 | 5.4:-4851828 | MS.gene019286:CDS |
| ATAGTCGAAACATGCTCTTC+GGG | 0.403328 | 5.4:-4856522 | MS.gene019286:intron |
| GAGGAGGAGTGCAGCACAAA+TGG | 0.404625 | 5.4:-4851912 | MS.gene019286:CDS |
| GACTCGGCAGGCTTTAACAT+TGG | 0.404721 | 5.4:-4856776 | MS.gene019286:CDS |
| ATTAGTCAGCTTGGTCCTCT+TGG | 0.410191 | 5.4:+4851841 | None:intergenic |
| TATAGTCGAAACATGCTCTT+CGG | 0.434439 | 5.4:-4856523 | MS.gene019286:intron |
| TGGGCACTTTAGGATCATTA+GGG | 0.444208 | 5.4:+4852053 | None:intergenic |
| TATAGGCTTTGAAAGGTGGC+AGG | 0.480454 | 5.4:-4852123 | MS.gene019286:intron |
| GCTGTCCAACTTTGATTCAC+AGG | 0.485843 | 5.4:-4852089 | MS.gene019286:CDS |
| GTCTTCCTGTGAATCAAAGT+TGG | 0.504653 | 5.4:+4852084 | None:intergenic |
| GAAATTCAGATGGCGTTACA+AGG | 0.505735 | 5.4:-4859565 | None:intergenic |
| GGATTCATACCCAACTGTAA+TGG | 0.506042 | 5.4:-4856435 | MS.gene019286:CDS |
| TTCAGTTTATAGGCTTTGAA+AGG | 0.517712 | 5.4:-4852130 | MS.gene019286:intron |
| TGTGCCAAATGCTCTTGCTC+CGG | 0.535916 | 5.4:-4859458 | MS.gene019286:CDS |
| TGTAAGTCCTCCATTACAGT+TGG | 0.544872 | 5.4:+4856425 | None:intergenic |
| ATCAAGCATAGCAGCAAACA+AGG | 0.552309 | 5.4:-4859494 | MS.gene019286:CDS |
| ATGTTGAAGTCTCTCTGTAT+CGG | 0.571558 | 5.4:+4859423 | None:intergenic |
| GTGATATGATTGATATAGAG+AGG | 0.575017 | 5.4:-4851979 | MS.gene019286:CDS |
| AGTATTCGTAAAGCTCCTGT+TGG | 0.575171 | 5.4:-4859394 | MS.gene019286:CDS |
| AAGATGGGCATTAGTCAGCT+TGG | 0.579562 | 5.4:+4851832 | None:intergenic |
| CTGATACGCAAATTTCCAGA+AGG | 0.581325 | 5.4:-4856479 | MS.gene019286:CDS |
| AGTTTATAGGCTTTGAAAGG+TGG | 0.583829 | 5.4:-4852127 | MS.gene019286:intron |
| TCCTAAAGTGCCCATTAAGG+TGG | 0.592622 | 5.4:-4852044 | MS.gene019286:CDS |
| TGGAGAAAGATTCTCTTACA+AGG | 0.595910 | 5.4:-4856665 | MS.gene019286:intron |
| AATCCAAAAGTAAGATACGA+TGG | 0.596900 | 5.4:-4856685 | MS.gene019286:CDS |
| TGTTAGCTTTCATGTCCACA+TGG | 0.600643 | 5.4:+4856734 | None:intergenic |
| GTAAGTCCTCCATTACAGTT+GGG | 0.602392 | 5.4:+4856426 | None:intergenic |
| AGGAGTGCAGCACAAATGGA+AGG | 0.606142 | 5.4:-4851908 | MS.gene019286:CDS |
| GTATCGGTCGAAAATTTGAC+CGG | 0.607296 | 5.4:+4859439 | None:intergenic |
| CACTTTAGGATCATTAGGGT+AGG | 0.617730 | 5.4:+4852057 | None:intergenic |
| TTCATACCCAACTGTAATGG+AGG | 0.622275 | 5.4:-4856432 | MS.gene019286:CDS |
| TGATATGATTGATATAGAGA+GGG | 0.634684 | 5.4:-4851978 | MS.gene019286:CDS |
| ATAGGCTTTGAAAGGTGGCA+GGG | 0.637475 | 5.4:-4852122 | MS.gene019286:intron |
| CTACTAACACTGCAAAGAGG+AGG | 0.649367 | 5.4:-4851928 | MS.gene019286:CDS |
| AATAAATGAAACATGCCATG+TGG | 0.663478 | 5.4:-4856749 | MS.gene019286:CDS |
| AGAAGAATGAAATCACCAAG+AGG | 0.680362 | 5.4:-4851856 | MS.gene019286:CDS |
| CTGCTACTAACACTGCAAAG+AGG | 0.683295 | 5.4:-4851931 | MS.gene019286:CDS |
| TCTATATCAATCATATCACG+AGG | 0.737039 | 5.4:+4851983 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 4851799 | 4859578 | 4851799 | ID=MS.gene019286 |
| chr5.4 | mRNA | 4851799 | 4859578 | 4851799 | ID=MS.gene019286.t1;Parent=MS.gene019286 |
| chr5.4 | exon | 4859357 | 4859578 | 4859357 | ID=MS.gene019286.t1.exon1;Parent=MS.gene019286.t1 |
| chr5.4 | CDS | 4859357 | 4859578 | 4859357 | ID=cds.MS.gene019286.t1;Parent=MS.gene019286.t1 |
| chr5.4 | exon | 4856666 | 4856797 | 4856666 | ID=MS.gene019286.t1.exon2;Parent=MS.gene019286.t1 |
| chr5.4 | CDS | 4856666 | 4856797 | 4856666 | ID=cds.MS.gene019286.t1;Parent=MS.gene019286.t1 |
| chr5.4 | exon | 4856424 | 4856540 | 4856424 | ID=MS.gene019286.t1.exon3;Parent=MS.gene019286.t1 |
| chr5.4 | CDS | 4856424 | 4856540 | 4856424 | ID=cds.MS.gene019286.t1;Parent=MS.gene019286.t1 |
| chr5.4 | exon | 4851799 | 4852140 | 4851799 | ID=MS.gene019286.t1.exon4;Parent=MS.gene019286.t1 |
| chr5.4 | CDS | 4851799 | 4852140 | 4851799 | ID=cds.MS.gene019286.t1;Parent=MS.gene019286.t1 |
| Gene Sequence |
| Protein sequence |
