AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82237

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82237.t1 MTR_4g029010 99.500 200 1 0 1 200 1 200 3.06e-147 408
MS.gene82237.t1 MTR_7g072580 94.500 200 11 0 1 200 1 200 3.01e-139 387
MS.gene82237.t1 MTR_7g072580 94.500 200 11 0 1 200 1 200 3.78e-138 386
MS.gene82237.t1 MTR_4g029010 98.621 145 1 1 1 145 1 144 7.60e-102 291
MS.gene82237.t1 MTR_4g029010 99.296 142 1 0 1 142 1 142 2.21e-101 291
MS.gene82237.t1 MTR_3g065900 69.458 203 62 0 1 203 1 203 1.85e-98 285
MS.gene82237.t1 MTR_7g072580 95.070 142 7 0 1 142 1 142 1.92e-96 278
MS.gene82237.t1 MTR_3g066070 68.306 183 58 0 21 203 54 236 1.78e-84 250
MS.gene82237.t1 MTR_3g065900 62.000 200 59 1 1 200 15 197 8.06e-80 237
MS.gene82237.t1 MTR_3g066070 68.571 175 52 1 26 200 59 230 2.40e-77 232
MS.gene82237.t1 MTR_3g066070 68.571 175 52 1 26 200 59 230 2.40e-77 232
MS.gene82237.t1 MTR_3g066070 62.360 178 50 1 26 203 59 219 1.21e-69 212
MS.gene82237.t1 MTR_3g066070 61.714 175 50 1 26 200 10 167 5.01e-66 201
MS.gene82237.t1 MTR_3g066070 61.714 175 50 1 26 200 59 216 7.78e-66 202
MS.gene82237.t1 MTR_3g066070 61.714 175 50 1 26 200 59 216 7.78e-66 202
MS.gene82237.t1 MTR_3g066070 59.859 142 40 1 62 203 1 125 1.50e-50 160
MS.gene82237.t1 MTR_4g122610 54.348 138 35 5 1 111 1 137 9.12e-37 126
MS.gene82237.t1 MTR_3g066070 58.120 117 32 1 26 142 59 158 1.10e-36 126
MS.gene82237.t1 MTR_4g122635 48.571 140 46 5 6 142 1 117 4.91e-28 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82237.t1 AT1G55520 92.000 200 16 0 1 200 1 200 3.42e-134 375
MS.gene82237.t1 AT1G55520 92.000 200 16 0 1 200 1 200 3.42e-134 375
MS.gene82237.t1 AT1G55520 92.000 200 16 0 1 200 1 200 3.42e-134 375
MS.gene82237.t1 AT3G13445 90.500 200 19 0 1 200 1 200 1.06e-132 371
MS.gene82237.t1 AT1G55520 93.785 177 11 0 1 177 1 177 4.27e-120 338
MS.gene82237.t1 AT3G13445 89.730 185 19 0 1 185 1 185 8.82e-120 338

Find 52 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGCTTCAATGCTAGGTTTA+AGG 0.177730 4.2:+74999427 MS.gene82237:CDS
TATTGTTTCAACAGTCAATT+TGG 0.195167 4.2:+74998266 MS.gene82237:CDS
CATCTTTGCTTCTGGCAAAA+TGG 0.262684 4.2:+74998740 MS.gene82237:CDS
TATGAACCTGAGCTGTTTCC+CGG 0.266557 4.2:+74999943 MS.gene82237:CDS
TCTGGAAAGATTGTTCTTAC+AGG 0.298488 4.2:+75000015 MS.gene82237:CDS
TAGCATTGAAGCCAAGCTTT+TGG 0.302360 4.2:-74999418 None:intergenic
GATCTAGCGAAACATCCTTC+TGG 0.344452 4.2:+74997224 MS.gene82237:CDS
ACTGCACTCATCTTTGCTTC+TGG 0.346245 4.2:+74998732 MS.gene82237:CDS
ACCCTGTGCTCACCGAGTTC+AGG 0.348636 4.2:+75000173 MS.gene82237:CDS
CAAGACCCTCCAACCGTATT+GGG 0.367935 4.2:-74999630 None:intergenic
GAGTGAGCAACAGTCTAAAT+TGG 0.370609 4.2:+74999196 MS.gene82237:CDS
AAAATTAGCAAGACTATCTT+CGG 0.380946 4.2:-74999988 None:intergenic
ATTATGACAATCAGCATAGA+TGG 0.409040 4.2:-75000246 None:intergenic
TTCAAGATTCAGAACATTGT+TGG 0.416837 4.2:+74999589 MS.gene82237:CDS
GCAAGACCCTCCAACCGTAT+TGG 0.421333 4.2:-74999631 None:intergenic
TGTACTCTGCATTACGAGCT+TGG 0.428829 4.2:-74998320 None:intergenic
ATCTAGCGAAACATCCTTCT+GGG 0.437658 4.2:+74997225 MS.gene82237:CDS
AAAAGCTTGGCTTCAATGCT+AGG 0.438170 4.2:+74999420 MS.gene82237:CDS
GAGGGTCTTGCATATTCCCA+TGG 0.454651 4.2:+74999643 MS.gene82237:CDS
TTCCTGAACTCGGTGAGCAC+AGG 0.462497 4.2:-75000175 None:intergenic
AGTCAATTTGGACACTAAGT+TGG 0.477037 4.2:+74998278 MS.gene82237:CDS
AGGGTCTTGCATATTCCCAT+GGG 0.478905 4.2:+74999644 MS.gene82237:CDS
GTCTAAATTGGCAGCAAGGA+AGG 0.482016 4.2:+74999208 MS.gene82237:CDS
CTATCTTCGGTTTCTTCATA+CGG 0.487647 4.2:-74999975 None:intergenic
ATTAACCCGGGAAACAGCTC+AGG 0.491192 4.2:-74999949 None:intergenic
CTTCATACGGTAGATTAACC+CGG 0.497293 4.2:-74999962 None:intergenic
CAAGTTCCCAATACGGTTGG+AGG 0.507180 4.2:+74999624 MS.gene82237:CDS
GGATGTTTCGCTAGATCCAC+AGG 0.507959 4.2:-74997218 None:intergenic
TTAAGGTTGATGGCTGATCA+AGG 0.508347 4.2:+74997179 None:intergenic
GTGATGTCAAGTTCCCAATA+CGG 0.512187 4.2:+74999617 MS.gene82237:CDS
TTTGTATTATCAGGTGAGAG+AGG 0.516176 4.2:+75000123 MS.gene82237:intron
GCCCGTATCATCCAAAAGCT+TGG 0.528418 4.2:+74999407 MS.gene82237:CDS
TGGTATGACTAGGTTTGCAC+TGG 0.528649 4.2:+74999167 MS.gene82237:intron
TCCTGAACTCGGTGAGCACA+GGG 0.554650 4.2:-75000174 None:intergenic
GTTGATGGCTGATCAAGGAT+TGG 0.555740 4.2:+74997184 None:intergenic
ATGGCTGATCAAGGATTGGA+AGG 0.558814 4.2:+74997188 MS.gene82237:CDS
ATGAACCTGAGCTGTTTCCC+GGG 0.563246 4.2:+74999944 MS.gene82237:CDS
GATTGTTCTTACAGGAGCCA+AGG 0.563526 4.2:+75000023 MS.gene82237:CDS
AACAGTCTAAATTGGCAGCA+AGG 0.569501 4.2:+74999204 MS.gene82237:CDS
TTAGATACTCACTGAAGGGT+AGG 0.578649 4.2:-74997254 None:intergenic
CACAGGGTATATTTGCTCAA+AGG 0.582103 4.2:-75000158 None:intergenic
TACACTAGAGAAAGCCCCAT+GGG 0.590023 4.2:-74999659 None:intergenic
TGTCAAGTTCCCAATACGGT+TGG 0.593973 4.2:+74999621 MS.gene82237:CDS
TTCATACGGTAGATTAACCC+GGG 0.608069 4.2:-74999961 None:intergenic
GTTTCGCTAGATCCACAGGT+TGG 0.617374 4.2:-74997214 None:intergenic
AAGTTCCCAATACGGTTGGA+GGG 0.627507 4.2:+74999625 MS.gene82237:CDS
AGGGTAGGCACGATCCCAGA+AGG 0.629550 4.2:-74997239 None:intergenic
ATTGGAAGGGAGCCAACCTG+TGG 0.653530 4.2:+74997202 MS.gene82237:CDS
TAATGCAGAGTACAATCCCA+AGG 0.657375 4.2:+74998329 MS.gene82237:CDS
GGGTCTTGCATATTCCCATG+GGG 0.664391 4.2:+74999645 MS.gene82237:CDS
TGGCTGATCAAGGATTGGAA+GGG 0.672278 4.2:+74997189 MS.gene82237:CDS
TTACACTAGAGAAAGCCCCA+TGG 0.679761 4.2:-74999660 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTATATAAAATTATTACCT+TGG - chr4.2:75000043-75000062 None:intergenic 15.0%
!! TTATTATTTACTTTGAATTC+TGG + chr4.2:74998162-74998181 MS.gene82237:intron 15.0%
!!! ATTTATTTTGGTTTAGTAAA+AGG - chr4.2:74999090-74999109 None:intergenic 15.0%
!!! TATACATTTTTTGTATTATC+AGG + chr4.2:75000114-75000133 MS.gene82237:intron 15.0%
!! AACACAAAAATTTCAAAAAG+TGG - chr4.2:74998490-74998509 None:intergenic 20.0%
!! AAGCATAATAATTACTACAT+TGG - chr4.2:74998138-74998157 None:intergenic 20.0%
!! TATAACGTCAAAAATATTTG+AGG - chr4.2:74997930-74997949 None:intergenic 20.0%
!! TTGTAGTCAAATAAAAAGAT+TGG + chr4.2:74998825-74998844 MS.gene82237:intron 20.0%
!!! CAAATATTTTTGACGTTATA+TGG + chr4.2:74997930-74997949 MS.gene82237:intron 20.0%
!!! GTAAATTTTGAAAAATAAGC+AGG - chr4.2:74999346-74999365 None:intergenic 20.0%
!!! TGTACTGTATCAATTTATTT+TGG - chr4.2:74999102-74999121 None:intergenic 20.0%
! AAAATTAGCAAGACTATCTT+CGG - chr4.2:74999991-75000010 None:intergenic 25.0%
! AAGAAAGCTAAGCATAAATA+AGG - chr4.2:74997752-74997771 None:intergenic 25.0%
! AATATTTAGATACTCACTGA+AGG - chr4.2:74997262-74997281 None:intergenic 25.0%
! AATGACTTCCTAAATATCAT+TGG - chr4.2:74997816-74997835 None:intergenic 25.0%
! ATATTTAGATACTCACTGAA+GGG - chr4.2:74997261-74997280 None:intergenic 25.0%
! ATGACTTCCTAAATATCATT+GGG - chr4.2:74997815-74997834 None:intergenic 25.0%
! ATGATTTCCCAATGATATTT+AGG + chr4.2:74997805-74997824 MS.gene82237:intron 25.0%
! GTAAAAGGAACATTACATTA+CGG - chr4.2:74999075-74999094 None:intergenic 25.0%
! TAAGTATGGTGAAAAGATTT+AGG + chr4.2:74999743-74999762 MS.gene82237:intron 25.0%
! TATATGGAAGTAAACAAAAG+CGG + chr4.2:74997946-74997965 MS.gene82237:intron 25.0%
! TATTGTTTCAACAGTCAATT+TGG + chr4.2:74998266-74998285 MS.gene82237:CDS 25.0%
! TCAGTGAAGATAATCTTAAA+GGG - chr4.2:74999464-74999483 None:intergenic 25.0%
! TGGTAATTATTAGTGCATAT+AGG + chr4.2:74997678-74997697 MS.gene82237:intron 25.0%
! TTAAATGGAATGTGATAGAA+AGG + chr4.2:74997874-74997893 MS.gene82237:intron 25.0%
! TTCAGTGAAGATAATCTTAA+AGG - chr4.2:74999465-74999484 None:intergenic 25.0%
! TTGAGGGAAAAATTAACAAA+AGG - chr4.2:75000070-75000089 None:intergenic 25.0%
!! ATATACTGAACTTGCTTATA+TGG + chr4.2:74999912-74999931 MS.gene82237:intron 25.0%
!! GAAAGTGAAAATTAACTTGA+TGG + chr4.2:74998453-74998472 MS.gene82237:intron 25.0%
!! GAAATTTTTGTGTTTCTTTC+TGG + chr4.2:74998496-74998515 MS.gene82237:intron 25.0%
!! GTATTTGCAGAATATTGATT+TGG - chr4.2:74998617-74998636 None:intergenic 25.0%
!! TAATTTTTCCCTCAAAATCA+TGG + chr4.2:75000075-75000094 MS.gene82237:intron 25.0%
!! TATACTGAACTTGCTTATAT+GGG + chr4.2:74999913-74999932 MS.gene82237:intron 25.0%
!! TCATTTTCTCAAATAACTAC+CGG - chr4.2:74997438-74997457 None:intergenic 25.0%
!! TGATTTGGAATAACAAAAGA+AGG - chr4.2:74998602-74998621 None:intergenic 25.0%
!! TTATGCTGTTCTAATTTTAC+TGG + chr4.2:74999147-74999166 MS.gene82237:intron 25.0%
AAGAGCTGTAATATAGTTGT+TGG + chr4.2:74997779-74997798 MS.gene82237:intron 30.0%
AAGGAATCAACTACTAATCA+AGG - chr4.2:74999550-74999569 None:intergenic 30.0%
AAGTAACTCTACATGCAATA+TGG - chr4.2:74999847-74999866 None:intergenic 30.0%
AATTGATACAGTACATGTGA+AGG + chr4.2:74999107-74999126 MS.gene82237:intron 30.0%
ACTAACACAAATATGGACAT+AGG + chr4.2:74997390-74997409 MS.gene82237:intron 30.0%
AGTTCTGCATCATAAACATA+CGG - chr4.2:74998942-74998961 None:intergenic 30.0%
ATATGGAAGTAAACAAAAGC+GGG + chr4.2:74997947-74997966 MS.gene82237:intron 30.0%
ATATTGTGATTGTGATGTGA+TGG + chr4.2:74998013-74998032 MS.gene82237:intron 30.0%
ATTCATCCTAATCAACTTTG+AGG - chr4.2:74999296-74999315 None:intergenic 30.0%
ATTCTGTTACATTGCATCAA+TGG + chr4.2:74997658-74997677 MS.gene82237:intron 30.0%
GAGAAAATGAAGATTGATGA+TGG + chr4.2:74997533-74997552 MS.gene82237:intron 30.0%
GATTCAATCAAACGAACAAA+AGG - chr4.2:74997305-74997324 None:intergenic 30.0%
TCGTTTGATTGAATCATAGT+TGG + chr4.2:74997310-74997329 MS.gene82237:intron 30.0%
TGCTATTAATGTCTCTCTAA+CGG + chr4.2:74999266-74999285 MS.gene82237:intron 30.0%
TTCAAGATTCAGAACATTGT+TGG + chr4.2:74999589-74999608 MS.gene82237:CDS 30.0%
TTGATTGAATCATAGTTGGA+GGG + chr4.2:74997314-74997333 MS.gene82237:intron 30.0%
TTGTGTTAGAATGTAGATGA+TGG + chr4.2:74999815-74999834 MS.gene82237:intron 30.0%
TTTGATTGAATCATAGTTGG+AGG + chr4.2:74997313-74997332 MS.gene82237:intron 30.0%
! AAATTTTAAACTCCCAACCA+TGG - chr4.2:74999032-74999051 None:intergenic 30.0%
! AAGGTATCATTCAAGCAAAA+AGG + chr4.2:74997893-74997912 MS.gene82237:intron 30.0%
! AATAACTATGCCATTTGTGT+TGG + chr4.2:74999313-74999332 MS.gene82237:intron 30.0%
! ATGAAGCACTAACACAAATA+TGG + chr4.2:74997383-74997402 MS.gene82237:intron 30.0%
! CTAATTTTACTGGTATGACT+AGG + chr4.2:74999157-74999176 MS.gene82237:intron 30.0%
! TGAATAGTCGATTCTTGAAT+CGG - chr4.2:74997621-74997640 None:intergenic 30.0%
! TGATTAGTAGTTGATTCCTT+TGG + chr4.2:74999550-74999569 MS.gene82237:intron 30.0%
! TTGATTTGATTCACTCTGTA+TGG + chr4.2:74999006-74999025 MS.gene82237:intron 30.0%
!! AAAAATTTTAGCCTCAATCC+AGG - chr4.2:74997564-74997583 None:intergenic 30.0%
!! GTCTTGCTAATTTTTGTTTC+TGG + chr4.2:74999997-75000016 MS.gene82237:CDS 30.0%
!! TAGAATGTAGATGATGGAAA+GGG + chr4.2:74999821-74999840 MS.gene82237:intron 30.0%
!! TTAGAATGTAGATGATGGAA+AGG + chr4.2:74999820-74999839 MS.gene82237:intron 30.0%
!!! TTGATTCTCTTTTAGAATCC+TGG + chr4.2:74998883-74998902 MS.gene82237:intron 30.0%
AAATTATTCGACTAAGCACG+CGG + chr4.2:74997850-74997869 MS.gene82237:intron 35.0%
AACATTACATTACGGACATG+AGG - chr4.2:74999067-74999086 None:intergenic 35.0%
AACTATATTACAGCTCTTCC+CGG - chr4.2:74997777-74997796 None:intergenic 35.0%
ACAACCAAAGCAATGGAAAA+AGG - chr4.2:74998433-74998452 None:intergenic 35.0%
ACATTACATTACGGACATGA+GGG - chr4.2:74999066-74999085 None:intergenic 35.0%
AGCAATGGAAAAAGGAAACA+TGG - chr4.2:74998425-74998444 None:intergenic 35.0%
AGGAAAAATCAGCAATGGTA+TGG + chr4.2:75000193-75000212 MS.gene82237:CDS 35.0%
AGTCAATTTGGACACTAAGT+TGG + chr4.2:74998278-74998297 MS.gene82237:CDS 35.0%
AGTTCAGGAAAAATCAGCAA+TGG + chr4.2:75000188-75000207 MS.gene82237:CDS 35.0%
CAAGAGATTAAACTTGCCTT+GGG - chr4.2:74998348-74998367 None:intergenic 35.0%
CATATAATGCACACACAACA+CGG - chr4.2:74997486-74997505 None:intergenic 35.0%
CTATCTTCGGTTTCTTCATA+CGG - chr4.2:74999978-74999997 None:intergenic 35.0%
GATTTGTATGGGGATAAGTA+TGG + chr4.2:74999729-74999748 MS.gene82237:intron 35.0%
GCAATGGAAAAAGGAAACAT+GGG - chr4.2:74998424-74998443 None:intergenic 35.0%
GTACTACTGTGAAACTGAAA+CGG - chr4.2:74999517-74999536 None:intergenic 35.0%
TATTAATGTCTCTCTAACGG+AGG + chr4.2:74999269-74999288 MS.gene82237:intron 35.0%
TATTAGTGCATATAGGCAAG+TGG + chr4.2:74997685-74997704 MS.gene82237:intron 35.0%
TCAAGAGATTAAACTTGCCT+TGG - chr4.2:74998349-74998368 None:intergenic 35.0%
TCTGGAAAGATTGTTCTTAC+AGG + chr4.2:75000015-75000034 MS.gene82237:CDS 35.0%
TTCTGCAAGTAAACAGACAT+TGG - chr4.2:74998245-74998264 None:intergenic 35.0%
TTTCTCCATGCAAAATCACA+AGG - chr4.2:74997519-74997538 None:intergenic 35.0%
TTTGTATTATCAGGTGAGAG+AGG + chr4.2:75000123-75000142 MS.gene82237:intron 35.0%
! CATTGGTAGTAAACAGACAT+TGG - chr4.2:74998121-74998140 None:intergenic 35.0%
! GTTTCCTTTTTCCATTGCTT+TGG + chr4.2:74998426-74998445 MS.gene82237:intron 35.0%
! TTATGCTTAGCTTTCTTGTC+CGG + chr4.2:74997755-74997774 MS.gene82237:intron 35.0%
!! AAGATGAGTGCAGTTGTTTT+TGG - chr4.2:74998726-74998745 None:intergenic 35.0%
!! AGATTGATGATGGATTTACC+TGG + chr4.2:74997543-74997562 MS.gene82237:intron 35.0%
!! GAAAAGCACTTATATGACAG+AGG - chr4.2:74998790-74998809 None:intergenic 35.0%
!!! TTCTCTTTTAGAATCCTGGT+AGG + chr4.2:74998887-74998906 MS.gene82237:intron 35.0%
!!! TTGCTGATTTTTCCTGAACT+CGG - chr4.2:75000188-75000207 None:intergenic 35.0%
AAAAGCTTGGCTTCAATGCT+AGG + chr4.2:74999420-74999439 MS.gene82237:CDS 40.0%
AACAGTCTAAATTGGCAGCA+AGG + chr4.2:74999204-74999223 MS.gene82237:CDS 40.0%
AAGCACTTATATGACAGAGG+TGG - chr4.2:74998787-74998806 None:intergenic 40.0%
ATCCTGAAGCATAAAGCCAA+AGG - chr4.2:74999569-74999588 None:intergenic 40.0%
ATCTAGCGAAACATCCTTCT+GGG + chr4.2:74997225-74997244 MS.gene82237:CDS 40.0%
CACAGGGTATATTTGCTCAA+AGG - chr4.2:75000161-75000180 None:intergenic 40.0%
CACTTTCACAACCAAAGCAA+TGG - chr4.2:74998440-74998459 None:intergenic 40.0%
CATCTTTGCTTCTGGCAAAA+TGG + chr4.2:74998740-74998759 MS.gene82237:CDS 40.0%
CTTCATACGGTAGATTAACC+CGG - chr4.2:74999965-74999984 None:intergenic 40.0%
GAGTGAGCAACAGTCTAAAT+TGG + chr4.2:74999196-74999215 MS.gene82237:CDS 40.0%
GTGATGTCAAGTTCCCAATA+CGG + chr4.2:74999617-74999636 MS.gene82237:CDS 40.0%
GTGTGTGCATTATATGCTGA+TGG + chr4.2:74997490-74997509 MS.gene82237:intron 40.0%
TAATGCAGAGTACAATCCCA+AGG + chr4.2:74998329-74998348 MS.gene82237:CDS 40.0%
TACCATAATAGTCTCTCCCA+CGG - chr4.2:74997711-74997730 None:intergenic 40.0%
TGGCTTCAATGCTAGGTTTA+AGG + chr4.2:74999427-74999446 MS.gene82237:CDS 40.0%
TTAGATACTCACTGAAGGGT+AGG - chr4.2:74997257-74997276 None:intergenic 40.0%
TTCATACGGTAGATTAACCC+GGG - chr4.2:74999964-74999983 None:intergenic 40.0%
TTCTCAAATAACTACCGGTG+TGG - chr4.2:74997433-74997452 None:intergenic 40.0%
! AGCCAAGCTTTTGGATGATA+CGG - chr4.2:74999412-74999431 None:intergenic 40.0%
! ATCGCTTGACGTGATTTGTA+TGG + chr4.2:74999717-74999736 MS.gene82237:intron 40.0%
! CGAGTGAACCATGATTTTGA+GGG - chr4.2:75000086-75000105 None:intergenic 40.0%
! CTATGCCATTTGTGTTGGAT+TGG + chr4.2:74999318-74999337 MS.gene82237:intron 40.0%
! CTGAACTTGCTTATATGGGA+TGG + chr4.2:74999917-74999936 MS.gene82237:intron 40.0%
! GGTTACCTTGTGATTTTGCA+TGG + chr4.2:74997511-74997530 MS.gene82237:intron 40.0%
! GTTTTGCTGCTGTCATTATG+AGG + chr4.2:74998691-74998710 MS.gene82237:CDS 40.0%
! TATGCTTAGCTTTCTTGTCC+GGG + chr4.2:74997756-74997775 MS.gene82237:intron 40.0%
! TCGAGTGAACCATGATTTTG+AGG - chr4.2:75000087-75000106 None:intergenic 40.0%
! TCGCTTGACGTGATTTGTAT+GGG + chr4.2:74999718-74999737 MS.gene82237:intron 40.0%
!! TAGCATTGAAGCCAAGCTTT+TGG - chr4.2:74999421-74999440 None:intergenic 40.0%
!! TGATGGATTTACCTGGATTG+AGG + chr4.2:74997550-74997569 MS.gene82237:intron 40.0%
!! TTCCTTTGGCTTTATGCTTC+AGG + chr4.2:74999564-74999583 MS.gene82237:intron 40.0%
!!! TCTTTTAGAATCCTGGTAGG+AGG + chr4.2:74998890-74998909 MS.gene82237:intron 40.0%
AAGTTCCCAATACGGTTGGA+GGG + chr4.2:74999625-74999644 MS.gene82237:CDS 45.0%
ACTGCACTCATCTTTGCTTC+TGG + chr4.2:74998732-74998751 MS.gene82237:CDS 45.0%
AGGCTCCAATCCAACACAAA+TGG - chr4.2:74999326-74999345 None:intergenic 45.0%
AGGGTCTTGCATATTCCCAT+GGG + chr4.2:74999644-74999663 MS.gene82237:CDS 45.0%
ATGGCTGATCAAGGATTGGA+AGG + chr4.2:74997188-74997207 MS.gene82237:CDS 45.0%
GACTAAGCACGCGGTTTAAA+TGG + chr4.2:74997859-74997878 MS.gene82237:intron 45.0%
GATCTAGCGAAACATCCTTC+TGG + chr4.2:74997224-74997243 MS.gene82237:CDS 45.0%
GATTGTTCTTACAGGAGCCA+AGG + chr4.2:75000023-75000042 MS.gene82237:CDS 45.0%
GGAAAAAGGAAACATGGGCA+AGG - chr4.2:74998419-74998438 None:intergenic 45.0%
GTCTAAATTGGCAGCAAGGA+AGG + chr4.2:74999208-74999227 MS.gene82237:CDS 45.0%
TACACTAGAGAAAGCCCCAT+GGG - chr4.2:74999662-74999681 None:intergenic 45.0%
TGATTCACTCTGTATGGCCA+TGG + chr4.2:74999012-74999031 MS.gene82237:intron 45.0%
TGGCTGATCAAGGATTGGAA+GGG + chr4.2:74997189-74997208 MS.gene82237:CDS 45.0%
TGGTATGACTAGGTTTGCAC+TGG + chr4.2:74999167-74999186 MS.gene82237:intron 45.0%
TGTACTCTGCATTACGAGCT+TGG - chr4.2:74998323-74998342 None:intergenic 45.0%
TGTCAAGTTCCCAATACGGT+TGG + chr4.2:74999621-74999640 MS.gene82237:CDS 45.0%
TTACACTAGAGAAAGCCCCA+TGG - chr4.2:74999663-74999682 None:intergenic 45.0%
TTAGAATCCTGGTAGGAGGA+GGG + chr4.2:74998894-74998913 MS.gene82237:intron 45.0%
! CGCTTGACGTGATTTGTATG+GGG + chr4.2:74999719-74999738 MS.gene82237:intron 45.0%
! GCCAAGCTTTTGGATGATAC+GGG - chr4.2:74999411-74999430 None:intergenic 45.0%
! GGAGGACCTCAAAGTTGATT+AGG + chr4.2:74999287-74999306 MS.gene82237:intron 45.0%
! TATGAACCTGAGCTGTTTCC+CGG + chr4.2:74999943-74999962 MS.gene82237:CDS 45.0%
! TTTAGAATCCTGGTAGGAGG+AGG + chr4.2:74998893-74998912 MS.gene82237:intron 45.0%
ATTAACCCGGGAAACAGCTC+AGG - chr4.2:74999952-74999971 None:intergenic 50.0%
CAAGACCCTCCAACCGTATT+GGG - chr4.2:74999633-74999652 None:intergenic 50.0%
CAAGTTCCCAATACGGTTGG+AGG + chr4.2:74999624-74999643 MS.gene82237:CDS 50.0%
CACTCTGTATGGCCATGGTT+GGG + chr4.2:74999017-74999036 MS.gene82237:intron 50.0%
GAGGGTCTTGCATATTCCCA+TGG + chr4.2:74999643-74999662 MS.gene82237:CDS 50.0%
GCCCGTATCATCCAAAAGCT+TGG + chr4.2:74999407-74999426 MS.gene82237:CDS 50.0%
GGATGTTTCGCTAGATCCAC+AGG - chr4.2:74997221-74997240 None:intergenic 50.0%
GGGTCTTGCATATTCCCATG+GGG + chr4.2:74999645-74999664 MS.gene82237:CDS 50.0%
GTTTCGCTAGATCCACAGGT+TGG - chr4.2:74997217-74997236 None:intergenic 50.0%
TCACTCTGTATGGCCATGGT+TGG + chr4.2:74999016-74999035 MS.gene82237:intron 50.0%
TGACATTGACACGTCCACAC+CGG + chr4.2:74997416-74997435 MS.gene82237:intron 50.0%
! ATGAACCTGAGCTGTTTCCC+GGG + chr4.2:74999944-74999963 MS.gene82237:CDS 50.0%
ATTGGAAGGGAGCCAACCTG+TGG + chr4.2:74997202-74997221 MS.gene82237:CDS 55.0%
CTAATGACCCTCCTCCTACC+AGG - chr4.2:74998904-74998923 None:intergenic 55.0%
GCAAGACCCTCCAACCGTAT+TGG - chr4.2:74999634-74999653 None:intergenic 55.0%
GCATATAGGCAAGTGGCCGT+GGG + chr4.2:74997692-74997711 MS.gene82237:intron 55.0%
GGCCGTGGGAGAGACTATTA+TGG + chr4.2:74997706-74997725 MS.gene82237:intron 55.0%
TGCATATAGGCAAGTGGCCG+TGG + chr4.2:74997691-74997710 MS.gene82237:intron 55.0%
! TCCTGAACTCGGTGAGCACA+GGG - chr4.2:75000177-75000196 None:intergenic 55.0%
! TTCCTGAACTCGGTGAGCAC+AGG - chr4.2:75000178-75000197 None:intergenic 55.0%
ACCCTGTGCTCACCGAGTTC+AGG + chr4.2:75000173-75000192 MS.gene82237:CDS 60.0%
AGGGTAGGCACGATCCCAGA+AGG - chr4.2:74997242-74997261 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 74997188 75000258 74997188 ID=MS.gene82237
chr4.2 mRNA 74997188 75000258 74997188 ID=MS.gene82237.t1;Parent=MS.gene82237
chr4.2 exon 74997188 74997264 74997188 ID=MS.gene82237.t1.exon1;Parent=MS.gene82237.t1
chr4.2 CDS 74997188 74997264 74997188 ID=cds.MS.gene82237.t1;Parent=MS.gene82237.t1
chr4.2 exon 74998263 74998350 74998263 ID=MS.gene82237.t1.exon2;Parent=MS.gene82237.t1
chr4.2 CDS 74998263 74998350 74998263 ID=cds.MS.gene82237.t1;Parent=MS.gene82237.t1
chr4.2 exon 74998690 74998761 74998690 ID=MS.gene82237.t1.exon3;Parent=MS.gene82237.t1
chr4.2 CDS 74998690 74998761 74998690 ID=cds.MS.gene82237.t1;Parent=MS.gene82237.t1
chr4.2 exon 74999179 74999229 74999179 ID=MS.gene82237.t1.exon4;Parent=MS.gene82237.t1
chr4.2 CDS 74999179 74999229 74999179 ID=cds.MS.gene82237.t1;Parent=MS.gene82237.t1
chr4.2 exon 74999404 74999448 74999404 ID=MS.gene82237.t1.exon5;Parent=MS.gene82237.t1
chr4.2 CDS 74999404 74999448 74999404 ID=cds.MS.gene82237.t1;Parent=MS.gene82237.t1
chr4.2 exon 74999586 74999678 74999586 ID=MS.gene82237.t1.exon6;Parent=MS.gene82237.t1
chr4.2 CDS 74999586 74999678 74999586 ID=cds.MS.gene82237.t1;Parent=MS.gene82237.t1
chr4.2 exon 74999943 75000044 74999943 ID=MS.gene82237.t1.exon7;Parent=MS.gene82237.t1
chr4.2 CDS 74999943 75000044 74999943 ID=cds.MS.gene82237.t1;Parent=MS.gene82237.t1
chr4.2 exon 75000136 75000258 75000136 ID=MS.gene82237.t1.exon8;Parent=MS.gene82237.t1
chr4.2 CDS 75000136 75000258 75000136 ID=cds.MS.gene82237.t1;Parent=MS.gene82237.t1
Gene Sequence

>MS.gene82237

ATGGCTGATCAAGGATTGGAAGGGAGCCAACCTGTGGATCTAGCGAAACATCCTTCTGGGATCGTGCCTACCCTTCAGTGAGTATCTAAATATTAGTGTATATATAAATATTAGCCTTTTGTTCGTTTGATTGAATCATAGTTGGAGGGTTTGCTTTTATGTTTTTGAGATTATAAGTGATTTGAAATTGTTAGTATGAAGCACTAACACAAATATGGACATAGGACATGACATTGACACGTCCACACCGGTAGTTATTTGAGAAAATGACATAATTGAATGTAATCACATGTGTCCGTGTTGTGTGTGCATTATATGCTGATGGTTACCTTGTGATTTTGCATGGAGAAAATGAAGATTGATGATGGATTTACCTGGATTGAGGCTAAAATTTTTATTTGTTAATATTTTTTTTTTTGAAAACTCGTATCCGATTCAAGAATCGACTATTCATTTTATTTGTTAATATTATTCTGTTACATTGCATCAATGGTAATTATTAGTGCATATAGGCAAGTGGCCGTGGGAGAGACTATTATGGTATTGTATTATTGTTTGTATCCTTATTTATGCTTAGCTTTCTTGTCCGGGAAGAGCTGTAATATAGTTGTTGGTCGATGATTTCCCAATGATATTTAGGAAGTCATTAATTATAATAAGCAAAATTATTCGACTAAGCACGCGGTTTAAATGGAATGTGATAGAAAGGTATCATTCAAGCAAAAAGGAAATTTTTTTACCTCAAATATTTTTGACGTTATATGGAAGTAAACAAAAGCGGGAAAGAGTTAACTAGAAAATAAGTTGAACATTGTAGAGATAAAAATATTGTGATTGTGATGTGATGGATGAAAATAAGTTGAACGTATGCTTGAGCTTAGCTTTAGTGCACTTTGTTTACTTCATATTACATTACAATATCTAATACTACCAATGTCTGTTTACTACCAATGTAGTAATTATTATGCTTTTAGTTATTATTTACTTTGAATTCTGGAACATATAATAAATTTGCTTCTTTAATTTTTTTCTATTATTTAATTTAAACTAATTACCAATGTCTGTTTACTTGCAGAAATATTGTTTCAACAGTCAATTTGGACACTAAGTTGGATCTCAAAGCAATTGCACTCCAAGCTCGTAATGCAGAGTACAATCCCAAGGCAAGTTTAATCTCTTGACTGTCAATTTCATTCAAATACATCGATGATATGTGTGAAAGTGTGTGCCTTGCCCATGTTTCCTTTTTCCATTGCTTTGGTTGTGAAAGTGAAAATTAACTTGATGGTTGTACTGTTTCCACTTTTTGAAATTTTTGTGTTTCTTTCTGGAATAGAGATGCTTAGTTGATCTAATTATTAATCACTATTATTTTATATTAATGTCACTTTTGATATCAGTTACTTGTCGTCCTTCTTTTGTTATTCCAAATCAATATTCTGCAAATACAGTTAATATGCGTGTTTTAGTGTTACTGATATTTGTGATGCAATTGTCTGCAGCGTTTTGCTGCTGTCATTATGAGGATCAGAGAACCAAAAACAACTGCACTCATCTTTGCTTCTGGCAAAATGGTATGCTTCTTTGTTGCACTCGCCACCTCTGTCATATAAGTGCTTTTCTTTGTGATTTTAGCATTGTAGTCAAATAAAAAGATTGGAAAGTTCTGTATTGTTTATGCTATTTTTTAGCTGTTTGATTCTCTTTTAGAATCCTGGTAGGAGGAGGGTCATTAGAGACTCTTCTTTTAGCCGTATGTTTATGATGCAGAACTATATTTTTTGTTATTTTAATCATTTAGCACTAGACTTCTAATACTTGATTTGATTCACTCTGTATGGCCATGGTTGGGAGTTTAAAATTTCAGTTTTTTTTCCCTCATGTCCGTAATGTAATGTTCCTTTTACTAAACCAAAATAAATTGATACAGTACATGTGAAGGAATTTTTTTTTTTTTTTTTATGCTGTTCTAATTTTACTGGTATGACTAGGTTTGCACTGGAGCTAAGAGTGAGCAACAGTCTAAATTGGCAGCAAGGAAGGTGTGTTCTTGTTCTTTTATTTGTTAATCTCATTCATGCTATTAATGTCTCTCTAACGGAGGACCTCAAAGTTGATTAGGATGAATAACTATGCCATTTGTGTTGGATTGGAGCCTGCTTATTTTTCAAAATTTACAGCATTTTCTAATTTTGTTTTTCTACTCTTTCTTTCAGTATGCCCGTATCATCCAAAAGCTTGGCTTCAATGCTAGGTTTAAGGTACAAAATCTACCCTTTAAGATTATCTTCACTGAAATTGTTTCATCAACTATAATTGATTTGTGCCGTTTCAGTTTCACAGTAGTACATTAGTAGTACCTTGATTAGTAGTTGATTCCTTTGGCTTTATGCTTCAGGATTTCAAGATTCAGAACATTGTTGGCTCTTGTGATGTCAAGTTCCCAATACGGTTGGAGGGTCTTGCATATTCCCATGGGGCTTTCTCTAGTGTAAGCTATTTTATTTACAGTTATCTTCACACTTCGTGATCGCTTGACGTGATTTGTATGGGGATAAGTATGGTGAAAAGATTTAGGTTTTTTCTGAAAATTTAAACTAAGCTCGTAGTGTTGTAAATAGTGATGCTTGTGTTAGAATGTAGATGATGGAAAGGGCCATATTGCATGTAGAGTTACTTGATTCAAGTCTTTTTATAAGTATTAATGTCTATAAGTTGAAGAAAATATACTGAACTTGCTTATATGGGATGGTAGTATGAACCTGAGCTGTTTCCCGGGTTAATCTACCGTATGAAGAAACCGAAGATAGTCTTGCTAATTTTTGTTTCTGGAAAGATTGTTCTTACAGGAGCCAAGGTAATAATTTTATATACAATCTCCTTTTGTTAATTTTTCCCTCAAAATCATGGTTCACTCGATTTTATGTATACATTTTTTGTATTATCAGGTGAGAGAGGAGACTTATCTAGCCTTTGAGCAAATATACCCTGTGCTCACCGAGTTCAGGAAAAATCAGCAATGGTATGGACATGACTTTATCTATTTTATTGCTGTTTACCATCTATGCTGA
Protein sequence

>MS.gene82237.t1

MADQGLEGSQPVDLAKHPSGIVPTLQNIVSTVNLDTKLDLKAIALQARNAEYNPKRFAAVIMRIREPKTTALIFASGKMVCTGAKSEQQSKLAARKYARIIQKLGFNARFKDFKIQNIVGSCDVKFPIRLEGLAYSHGAFSSYEPELFPGLIYRMKKPKIVLLIFVSGKIVLTGAKVREETYLAFEQIYPVLTEFRKNQQWYGHDFIYFIAVYHLC