AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene61081

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61081.t1 MTR_8g039540 97.005 434 13 0 1 434 1 434 0.0 857
MS.gene61081.t1 MTR_1g037630 82.692 416 71 1 19 434 15 429 0.0 691
MS.gene61081.t1 MTR_3g014290 80.769 416 67 2 19 434 15 417 0.0 662
MS.gene61081.t1 MTR_3g100500 54.875 441 187 7 1 433 1 437 6.12e-145 420
MS.gene61081.t1 MTR_3g097150 31.618 408 239 15 51 433 54 446 1.59e-53 185
MS.gene61081.t1 MTR_7g117430 31.915 423 261 11 28 433 72 484 9.96e-49 173
MS.gene61081.t1 MTR_1g111350 31.738 397 248 10 50 433 81 467 1.43e-48 172
MS.gene61081.t1 MTR_3g053710 32.468 385 226 14 66 428 89 461 1.52e-48 172
MS.gene61081.t1 MTR_1g115210 35.244 349 211 9 94 433 144 486 2.18e-48 172
MS.gene61081.t1 MTR_2g028190 32.346 439 237 19 35 433 33 451 4.47e-48 171
MS.gene61081.t1 MTR_4g007740 31.481 378 214 14 83 434 120 478 5.77e-45 163
MS.gene61081.t1 MTR_6g085010 32.948 346 212 10 97 433 157 491 3.95e-41 152
MS.gene61081.t1 MTR_8g103880 29.107 347 227 10 97 433 117 454 6.65e-41 151
MS.gene61081.t1 MTR_3g046300 32.061 393 230 12 71 433 73 458 9.81e-41 151
MS.gene61081.t1 MTR_5g091130 32.570 393 224 15 71 433 172 553 5.76e-40 150
MS.gene61081.t1 MTR_1g039270 33.048 351 217 9 94 433 126 469 6.59e-40 149
MS.gene61081.t1 MTR_4g100990 31.421 401 206 16 97 433 75 470 1.00e-38 146
MS.gene61081.t1 MTR_3g053980 33.516 364 211 15 83 428 130 480 2.49e-38 145
MS.gene61081.t1 MTR_0036s0110 27.570 428 270 16 32 434 29 441 6.60e-38 143
MS.gene61081.t1 MTR_0024s0040 30.969 423 255 16 35 434 1 409 6.05e-36 137
MS.gene61081.t1 MTR_5g038360 29.673 428 265 17 29 434 22 435 2.76e-34 133
MS.gene61081.t1 MTR_5g008110 31.486 397 226 19 72 434 30 414 6.32e-34 131
MS.gene61081.t1 MTR_5g012490 28.736 435 230 18 65 433 49 469 9.54e-34 132
MS.gene61081.t1 MTR_3g065160 29.477 363 219 15 98 434 76 427 6.33e-33 129
MS.gene61081.t1 MTR_7g062360 27.763 371 225 12 99 433 62 425 1.58e-32 128
MS.gene61081.t1 MTR_2g059460 28.261 368 228 16 92 434 49 405 2.55e-32 127
MS.gene61081.t1 MTR_8g096990 26.914 431 271 16 10 421 12 417 4.50e-32 127
MS.gene61081.t1 MTR_3g028120 27.378 431 272 16 29 434 23 437 1.07e-31 125
MS.gene61081.t1 MTR_5g077850 28.493 365 224 13 98 434 83 438 4.70e-31 124
MS.gene61081.t1 MTR_2g059490 27.624 362 237 14 92 434 54 409 5.91e-31 124
MS.gene61081.t1 MTR_0036s0070 26.733 404 263 13 32 421 28 412 1.35e-30 122
MS.gene61081.t1 MTR_7g105850 29.091 385 215 16 97 433 91 465 2.02e-30 122
MS.gene61081.t1 MTR_8g038280 27.494 451 273 19 10 434 12 434 8.40e-30 120
MS.gene61081.t1 MTR_5g008150 28.678 401 243 18 62 434 26 411 1.38e-29 119
MS.gene61081.t1 MTR_4g056420 27.470 415 242 20 61 434 41 437 1.87e-29 120
MS.gene61081.t1 MTR_5g080890 27.009 448 283 17 7 434 9 432 5.06e-29 118
MS.gene61081.t1 MTR_2g084550 27.027 407 254 15 61 434 42 438 3.15e-28 116
MS.gene61081.t1 MTR_5g080960 26.786 448 284 17 7 434 9 432 4.41e-28 115
MS.gene61081.t1 MTR_3g068000 26.963 382 222 15 96 433 73 441 8.59e-28 115
MS.gene61081.t1 MTR_0036s0050 26.887 424 272 16 32 434 28 434 1.27e-27 114
MS.gene61081.t1 MTR_5g016260 28.099 363 217 18 97 434 89 432 2.32e-27 115
MS.gene61081.t1 MTR_5g016260 28.099 363 217 18 97 434 89 432 2.45e-27 115
MS.gene61081.t1 MTR_8g038250 27.126 435 259 16 5 421 7 401 6.66e-27 112
MS.gene61081.t1 MTR_8g038310 27.842 431 256 18 5 421 10 399 7.91e-27 111
MS.gene61081.t1 MTR_5g077920 30.352 369 214 18 98 434 70 427 7.80e-26 109
MS.gene61081.t1 MTR_5g044770 26.879 346 226 12 92 421 82 416 1.84e-25 108
MS.gene61081.t1 MTR_5g044570 27.400 427 250 16 29 434 23 410 7.03e-25 106
MS.gene61081.t1 MTR_7g102750 27.200 375 221 18 97 434 82 441 5.28e-24 104
MS.gene61081.t1 MTR_1g060750 26.420 352 217 14 97 434 52 375 5.52e-24 103
MS.gene61081.t1 MTR_2g055840 27.446 368 228 12 97 433 14 373 8.51e-24 102
MS.gene61081.t1 MTR_2g070430 26.954 371 224 14 94 426 85 446 1.74e-23 102
MS.gene61081.t1 MTR_6g090290 27.111 450 283 18 7 434 9 435 2.40e-23 102
MS.gene61081.t1 MTR_1g061940 24.885 434 263 19 46 434 30 445 4.56e-23 101
MS.gene61081.t1 MTR_2g067830 27.500 360 227 15 97 434 58 405 7.95e-23 100
MS.gene61081.t1 MTR_5g022880 28.495 372 208 17 97 433 113 461 1.28e-22 100
MS.gene61081.t1 MTR_4g095270 26.111 360 226 16 97 433 81 423 1.29e-22 100
MS.gene61081.t1 MTR_4g095270 26.111 360 226 16 97 433 81 423 1.29e-22 100
MS.gene61081.t1 MTR_4g045857 31.982 222 130 10 89 289 45 266 3.04e-22 96.7
MS.gene61081.t1 MTR_5g008070 28.406 433 265 20 21 434 19 425 1.02e-21 97.1
MS.gene61081.t1 MTR_7g102750 29.375 320 181 16 97 382 82 390 2.42e-21 96.3
MS.gene61081.t1 MTR_7g080230 26.055 403 249 18 65 434 45 431 2.77e-21 96.3
MS.gene61081.t1 MTR_7g021510 25.806 372 232 13 97 434 73 434 3.15e-21 96.3
MS.gene61081.t1 MTR_6g088760 26.471 374 235 15 69 418 63 420 4.87e-21 95.9
MS.gene61081.t1 MTR_4g023650 27.441 379 212 17 97 433 62 419 1.79e-20 93.6
MS.gene61081.t1 MTR_5g061690 27.273 363 209 15 104 433 109 449 1.87e-19 90.9
MS.gene61081.t1 MTR_2g105300 26.888 331 208 15 118 426 57 375 3.09e-19 89.4
MS.gene61081.t1 MTR_4g109760 27.660 376 235 16 79 434 39 397 6.38e-18 85.5
MS.gene61081.t1 MTR_5g008090 28.808 302 191 12 149 434 20 313 9.52e-18 84.0
MS.gene61081.t1 MTR_3g096930 26.005 373 219 17 97 434 85 435 5.43e-17 83.2
MS.gene61081.t1 MTR_8g094590 25.296 423 255 19 41 427 21 418 6.21e-17 82.8
MS.gene61081.t1 MTR_1g078240 27.673 318 186 17 97 381 70 376 1.74e-16 81.3
MS.gene61081.t1 MTR_3g087780 25.745 369 210 18 104 433 107 450 6.50e-15 77.0
MS.gene61081.t1 MTR_8g094590 25.495 404 240 19 41 408 21 399 1.25e-14 75.9
MS.gene61081.t1 MTR_2g022450 23.430 414 238 17 65 432 34 414 4.17e-14 74.3
MS.gene61081.t1 MTR_8g094590 26.861 309 183 15 41 330 21 305 2.53e-13 70.9
MS.gene61081.t1 MTR_2g022480 22.404 366 223 15 104 420 57 410 2.61e-13 71.6
MS.gene61081.t1 MTR_1g072420 24.259 371 224 15 105 428 55 415 3.97e-13 71.2
MS.gene61081.t1 MTR_7g080230 26.852 324 200 16 137 434 49 361 5.16e-13 70.9
MS.gene61081.t1 MTR_1g072380 23.721 430 243 18 58 428 28 431 5.98e-13 70.9
MS.gene61081.t1 MTR_2g022430 24.274 379 231 16 97 428 40 409 9.58e-12 67.0
MS.gene61081.t1 MTR_1g093100 22.365 389 231 14 94 427 44 416 8.15e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61081.t1 AT5G07030 72.422 417 109 3 24 434 39 455 0.0 602
MS.gene61081.t1 AT3G54400 68.585 417 122 5 20 434 16 425 0.0 578
MS.gene61081.t1 AT1G09750 52.998 417 181 8 30 433 34 448 5.14e-143 416
MS.gene61081.t1 AT5G10770 32.867 429 247 16 33 434 60 474 7.14e-54 187
MS.gene61081.t1 AT5G10760 32.697 419 247 12 33 433 63 464 5.99e-51 179
MS.gene61081.t1 AT3G25700 33.171 410 230 14 61 433 47 449 2.07e-50 177
MS.gene61081.t1 AT2G03200 32.240 366 211 12 94 433 104 458 2.56e-49 174
MS.gene61081.t1 AT3G61820 33.989 356 215 7 94 433 132 483 7.67e-47 168
MS.gene61081.t1 AT1G01300 31.250 432 245 15 34 433 73 484 6.93e-46 166
MS.gene61081.t1 AT3G20015 30.693 404 252 9 53 433 72 470 2.73e-45 164
MS.gene61081.t1 AT1G25510 33.333 348 215 8 94 433 145 483 1.30e-43 159
MS.gene61081.t1 AT3G18490 33.056 360 222 11 84 433 150 500 2.04e-42 156
MS.gene61081.t1 AT1G79720 31.156 398 226 18 67 433 102 482 4.55e-42 155
MS.gene61081.t1 AT5G45120 30.424 401 210 17 97 433 83 478 2.39e-39 148
MS.gene61081.t1 AT1G66180 29.383 405 226 20 71 434 42 427 1.61e-37 142
MS.gene61081.t1 AT2G42980 30.311 386 232 13 80 433 144 524 5.33e-37 142
MS.gene61081.t1 AT3G52500 30.649 385 214 14 97 434 90 468 1.31e-36 140
MS.gene61081.t1 AT3G59080 28.995 438 245 14 58 433 99 532 7.47e-36 139
MS.gene61081.t1 AT5G37540 28.822 399 236 19 72 433 51 438 1.43e-33 131
MS.gene61081.t1 AT3G02740 28.607 402 243 16 62 434 54 440 1.07e-32 129
MS.gene61081.t1 AT2G39710 30.000 370 214 15 96 428 64 425 1.28e-32 128
MS.gene61081.t1 AT3G02740 29.428 367 219 16 97 434 10 365 5.22e-32 126
MS.gene61081.t1 AT5G22850 27.078 421 254 18 52 434 36 441 1.25e-30 123
MS.gene61081.t1 AT3G12700 28.708 418 246 18 51 434 62 461 1.74e-30 122
MS.gene61081.t1 AT5G02190 28.689 366 218 12 106 433 82 442 2.66e-30 122
MS.gene61081.t1 AT3G59080 28.140 430 223 14 58 433 99 496 3.33e-28 117
MS.gene61081.t1 AT2G23945 27.536 414 254 17 52 433 49 448 8.56e-28 115
MS.gene61081.t1 AT1G64830 26.761 426 270 15 31 434 24 429 7.73e-27 112
MS.gene61081.t1 AT4G30040 25.901 444 272 19 4 421 8 420 2.00e-26 110
MS.gene61081.t1 AT5G33340 26.667 345 227 11 93 420 86 421 9.24e-26 108
MS.gene61081.t1 AT4G30030 28.249 354 225 15 97 433 78 419 1.26e-25 108
MS.gene61081.t1 AT1G08210 29.087 416 250 18 52 434 38 441 1.27e-25 109
MS.gene61081.t1 AT1G08210 29.327 416 249 18 52 434 72 475 1.28e-25 109
MS.gene61081.t1 AT1G08210 29.087 416 250 18 52 434 86 489 1.37e-25 109
MS.gene61081.t1 AT5G43100 26.158 367 232 17 90 434 69 418 1.70e-25 108
MS.gene61081.t1 AT5G43100 26.158 367 232 17 90 434 69 418 1.75e-25 109
MS.gene61081.t1 AT2G36670 24.826 431 267 18 46 434 43 458 2.05e-25 108
MS.gene61081.t1 AT2G36670 24.658 438 264 19 46 434 43 463 2.34e-24 105
MS.gene61081.t1 AT2G36670 25.936 374 226 17 97 434 34 392 3.52e-24 104
MS.gene61081.t1 AT2G36670 25.936 374 226 17 97 434 34 392 3.52e-24 104
MS.gene61081.t1 AT1G08210 30.811 370 213 17 52 388 38 397 3.58e-23 102
MS.gene61081.t1 AT3G50050 26.374 364 223 17 97 434 93 437 2.78e-22 100
MS.gene61081.t1 AT2G28040 26.361 349 208 14 91 420 50 368 8.53e-22 97.1
MS.gene61081.t1 AT2G28040 26.361 349 208 14 91 420 59 377 1.10e-21 96.7
MS.gene61081.t1 AT5G36260 23.284 408 270 17 56 434 41 434 1.42e-21 97.4
MS.gene61081.t1 AT4G35880 26.796 362 197 18 97 420 107 438 2.49e-21 96.7
MS.gene61081.t1 AT4G16563 26.698 427 227 16 83 433 75 491 3.68e-20 93.2
MS.gene61081.t1 AT1G65240 24.740 384 241 16 83 434 60 427 9.02e-20 91.7
MS.gene61081.t1 AT2G28010 25.439 342 221 11 91 420 59 378 1.59e-19 90.5
MS.gene61081.t1 AT4G33490 27.568 370 209 19 97 429 60 407 1.81e-19 90.5
MS.gene61081.t1 AT2G28030 24.926 337 217 11 97 420 61 374 3.09e-19 89.4
MS.gene61081.t1 AT3G51350 27.704 379 204 19 97 433 102 452 4.89e-19 89.7
MS.gene61081.t1 AT3G51340 26.954 371 211 17 97 433 103 447 8.27e-19 89.0
MS.gene61081.t1 AT1G77480 27.660 376 210 18 97 433 67 419 2.07e-18 87.4
MS.gene61081.t1 AT1G77480 27.660 376 210 18 97 433 67 419 3.50e-18 87.0
MS.gene61081.t1 AT2G35615 26.685 371 228 15 97 434 85 444 6.85e-18 85.9
MS.gene61081.t1 AT1G31450 26.230 366 234 14 97 434 85 442 3.91e-17 83.6
MS.gene61081.t1 AT3G51360 25.124 402 250 15 62 433 65 445 4.47e-17 83.6
MS.gene61081.t1 AT3G51360 25.124 402 250 15 62 433 55 435 6.39e-17 83.2
MS.gene61081.t1 AT2G28225 27.195 353 204 17 97 426 67 389 6.57e-17 82.8
MS.gene61081.t1 AT3G51360 25.410 366 225 13 97 433 5 351 1.56e-16 81.6
MS.gene61081.t1 AT3G51330 27.855 359 192 19 104 421 109 441 2.83e-16 80.9
MS.gene61081.t1 AT4G33490 29.811 265 145 15 97 341 57 300 1.19e-15 79.0
MS.gene61081.t1 AT2G17760 24.682 393 231 17 80 433 83 449 7.73e-15 77.0
MS.gene61081.t1 AT3G25700 31.818 176 96 7 61 215 47 219 1.25e-14 75.5
MS.gene61081.t1 AT3G25700 37.594 133 71 4 309 433 219 347 7.61e-14 72.8
MS.gene61081.t1 AT3G51340 28.082 292 156 14 97 356 103 372 3.31e-14 74.3
MS.gene61081.t1 AT3G51340 28.082 292 156 14 97 356 103 372 3.56e-14 74.7
MS.gene61081.t1 AT3G51360 27.517 298 171 13 62 335 65 341 1.15e-13 72.4
MS.gene61081.t1 AT1G44130 27.177 379 208 20 97 434 49 400 1.46e-13 72.4
MS.gene61081.t1 AT3G42550 22.113 407 257 13 46 434 26 390 1.40e-12 69.7

Find 135 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCTTGGATGTCACATTT+TGG 0.163682 8.2:+60583506 None:intergenic
ATGTGGACTTGTAGAGATTT+TGG 0.241963 8.2:+60581185 None:intergenic
CTAAAACTTGTGGACTTAAC+AGG 0.288816 8.2:+60583161 None:intergenic
GTTATCGTTGGTACAACTAT+TGG 0.296291 8.2:+60579859 None:intergenic
GCTGCTTGGATTCCTTGTTC+TGG 0.307297 8.2:-60583218 MS.gene61081:CDS
TAATAGAGTGATGATCTTCT+AGG 0.307781 8.2:+60581055 None:intergenic
TTGGGGTTTGAGCTTGGACT+TGG 0.319192 8.2:+60583525 None:intergenic
CCAAAAGTGTACCTTGGAAT+TGG 0.323950 8.2:+60581295 None:intergenic
TTTCTTACGGCGGTGTATAC+AGG 0.325812 8.2:+60579955 None:intergenic
TACATTGTTAAGGCTAAGTT+TGG 0.332559 8.2:-60583287 MS.gene61081:CDS
TGTGCATTCAACTTCACTTA+TGG 0.334737 8.2:-60581373 MS.gene61081:CDS
TTTAAGTCGCCTAACTTTAG+TGG 0.335521 8.2:-60581142 MS.gene61081:CDS
ACCACCTCAAACTTTGCTTT+TGG 0.339719 8.2:-60583261 MS.gene61081:CDS
AGGGTTGTCACAGGTAGTTT+TGG 0.343997 8.2:+60579910 None:intergenic
CAACACCGAGTTCACGTTAT+CGG 0.358619 8.2:+60579738 None:intergenic
GCACCACAACAGGGTCTATT+GGG 0.360076 8.2:-60581244 MS.gene61081:CDS
CCGAGGCCCATTGTCGCTCT+TGG 0.369063 8.2:-60581215 MS.gene61081:CDS
GAGAACATTGGCTGAAGATA+TGG 0.376954 8.2:+60583462 None:intergenic
AACACCGAGTTCACGTTATC+GGG 0.382498 8.2:+60579739 None:intergenic
TTATCGTTGGTACAACTATT+GGG 0.382986 8.2:+60579860 None:intergenic
AGCACCACAACAGGGTCTAT+TGG 0.384446 8.2:-60581245 MS.gene61081:CDS
CGCCGGTAGCACCACATGTT+TGG 0.392098 8.2:-60579776 MS.gene61081:CDS
GGTCTATTGGGCTTGGGCCG+AGG 0.393741 8.2:-60581232 MS.gene61081:CDS
TGGGGTATACTTGATCCTCT+TGG 0.395670 8.2:+60581090 None:intergenic
CCAATTCCAAGGTACACTTT+TGG 0.404889 8.2:-60581295 MS.gene61081:CDS
GATGTGCCCAACTCAAGAAT+TGG 0.410152 8.2:-60579670 MS.gene61081:CDS
GGCTGTGTTAACAAAACAAC+AGG 0.414272 8.2:-60581274 MS.gene61081:CDS
TCAGTTGTACCAATTGCATC+AGG 0.418514 8.2:-60583335 MS.gene61081:CDS
CGATATCCCTCCGGCTGCGC+TGG 0.418711 8.2:-60580993 MS.gene61081:CDS
GGGGTATACTTGATCCTCTT+GGG 0.424317 8.2:+60581091 None:intergenic
AGGAAGTTCAGCACCACAAC+AGG 0.431654 8.2:-60581254 MS.gene61081:CDS
TTTAGTGGGTCGTTGAGACT+TGG 0.434229 8.2:-60581127 MS.gene61081:CDS
TTCAACCCAACTACCGGTGC+TGG 0.436030 8.2:-60580968 MS.gene61081:CDS
CTTTGGGCCAAGAGCGACAA+TGG 0.436884 8.2:+60581208 None:intergenic
AACTTGGTTGCTATTAAAGT+TGG 0.437202 8.2:-60581028 MS.gene61081:CDS
CTTCCACCGCAAGTGGGGTT+GGG 0.437902 8.2:+60581400 None:intergenic
ATGGTTCCAGCACCGGTAGT+TGG 0.439867 8.2:+60580962 None:intergenic
GGGTTGAACGCCAGCGCAGC+CGG 0.450344 8.2:+60580983 None:intergenic
TGGTTCCAGCACCGGTAGTT+GGG 0.453469 8.2:+60580963 None:intergenic
GGCCAAACATGTGGTGCTAC+CGG 0.458811 8.2:+60579774 None:intergenic
TGGGGTTTGAGCTTGGACTT+GGG 0.460621 8.2:+60583526 None:intergenic
ATCACTCTATTATGTTAACT+TGG 0.460738 8.2:-60581044 MS.gene61081:CDS
CAAAAGTGTACCTTGGAATT+GGG 0.463126 8.2:+60581296 None:intergenic
ATGATGAGGCAGAGAAGGAA+AGG 0.465733 8.2:+60583551 None:intergenic
CTTCTAGGGTTTCTGAGAAG+TGG 0.468084 8.2:+60581070 None:intergenic
GCTTCCACCGCAAGTGGGGT+TGG 0.468113 8.2:+60581399 None:intergenic
TGAGGATGTTGTCTGGTGGT+AGG 0.470698 8.2:+60579809 None:intergenic
AACAATACCAATTCTTGAGT+TGG 0.471847 8.2:+60579663 None:intergenic
ACCACATGTTTGGCCATGGC+AGG 0.475549 8.2:-60579766 MS.gene61081:CDS
TTCTAGGGTTTCTGAGAAGT+GGG 0.476620 8.2:+60581071 None:intergenic
ACAATACCAATTCTTGAGTT+GGG 0.478056 8.2:+60579664 None:intergenic
GTGGACTTAACAGGAGCAAA+AGG 0.478097 8.2:+60583170 None:intergenic
CCAAGAGCGACAATGGGCCT+CGG 0.482750 8.2:+60581215 None:intergenic
ATTTGTCTCCCTGATGCAAT+TGG 0.484764 8.2:+60583326 None:intergenic
ATCCTCTTGGGCTGATACAC+CGG 0.486552 8.2:+60581103 None:intergenic
TTGGAATTGGGTCAGTGGCT+AGG 0.489050 8.2:+60581308 None:intergenic
ACTTGTCCAGCTTAGTTGCT+AGG 0.489142 8.2:-60583361 MS.gene61081:CDS
TGGCTAGGGTAAGTGTGTCT+TGG 0.489193 8.2:+60581323 None:intergenic
ACGATAACATTCTTGTTCTC+CGG 0.489874 8.2:-60579844 MS.gene61081:CDS
TGGAATTGGGTCAGTGGCTA+GGG 0.491060 8.2:+60581309 None:intergenic
ACAACAGGGTCTATTGGGCT+TGG 0.499921 8.2:-60581239 MS.gene61081:CDS
CAACAGGGTCTATTGGGCTT+GGG 0.502732 8.2:-60581238 MS.gene61081:CDS
AACCATCAAAACCAATGTCA+TGG 0.503013 8.2:-60583433 MS.gene61081:CDS
TTGGACCACACTAGCTGCTA+TGG 0.505139 8.2:+60581342 None:intergenic
CTCAACGACCCACTAAAGTT+AGG 0.505593 8.2:+60581133 None:intergenic
ACAAGTATTGCATTCTAGCT+TGG 0.506286 8.2:+60583378 None:intergenic
AGCATGTGTCGAACCCTCCT+AGG 0.506559 8.2:+60579890 None:intergenic
AAGTCCAACATACATTGTTA+AGG 0.507953 8.2:-60583297 MS.gene61081:CDS
ACTAACATTCCTAAAACTTG+TGG 0.509218 8.2:+60583151 None:intergenic
ATTCCTTGTTCTGGTTGTGT+TGG 0.509240 8.2:-60583209 MS.gene61081:CDS
GAGGATGTTGTCTGGTGGTA+GGG 0.510247 8.2:+60579810 None:intergenic
TTAAGTCGCCTAACTTTAGT+GGG 0.511725 8.2:-60581141 MS.gene61081:CDS
TTAGTGGGTCGTTGAGACTT+GGG 0.514645 8.2:-60581126 MS.gene61081:CDS
CTATGTATGAGGATGTTGTC+TGG 0.515277 8.2:+60579802 None:intergenic
AATAGAGTGATGATCTTCTA+GGG 0.515833 8.2:+60581056 None:intergenic
TGGTTCTCCTCAGTGCAAGC+AGG 0.516456 8.2:-60583126 MS.gene61081:intron
TTAGCCTTAACAATGTATGT+TGG 0.520799 8.2:+60583293 None:intergenic
GGTCCTTAGCTTCTAGCTGT+AGG 0.521564 8.2:+60583399 None:intergenic
TTTGGGCCAAGAGCGACAAT+GGG 0.530197 8.2:+60581209 None:intergenic
TCGGCGAAACTCGTTTCTTA+CGG 0.530256 8.2:+60579942 None:intergenic
CACGCGCTTCCACCGCAAGT+GGG 0.533182 8.2:+60581394 None:intergenic
TGGGTCGTTGAGACTTGGGC+CGG 0.534117 8.2:-60581122 MS.gene61081:CDS
GGGAGATGATGAGGCAGAGA+AGG 0.536111 8.2:+60583546 None:intergenic
CTGGCGTTCAACCCAACTAC+CGG 0.536552 8.2:-60580974 MS.gene61081:CDS
TGATTTCCTAGCAACTAAGC+TGG 0.540455 8.2:+60583355 None:intergenic
AAATGTGACATCCAAGATGA+TGG 0.542321 8.2:-60583503 MS.gene61081:CDS
ATACTAGTAGTGATGCTGCT+TGG 0.543500 8.2:-60583232 MS.gene61081:CDS
ACCATCAAAACCAATGTCAT+GGG 0.556771 8.2:-60583432 MS.gene61081:CDS
TGGTTGCTATTAAAGTTGGC+CGG 0.557965 8.2:-60581024 MS.gene61081:CDS
ACACTTTCTTCCCATGACAT+TGG 0.559822 8.2:+60583422 None:intergenic
TGAGTGTTGAGCCATCATCT+TGG 0.560394 8.2:+60583492 None:intergenic
TTCTTCCATAGCAGCTAGTG+TGG 0.561075 8.2:-60581347 MS.gene61081:CDS
AAACTACCTGTGACAACCCT+AGG 0.568218 8.2:-60579907 MS.gene61081:CDS
TGTCCTACAGCTAGAAGCTA+AGG 0.572859 8.2:-60583402 MS.gene61081:CDS
GTTATCGGGTGCTCCTGCCA+TGG 0.574666 8.2:+60579753 None:intergenic
AACGAGTTTCGCCGACGAGT+TGG 0.576490 8.2:-60579934 MS.gene61081:CDS
AAGTTAGGCGACTTAAAGCT+AGG 0.577360 8.2:+60581148 None:intergenic
AACCCTCCTAGGGTTGTCAC+AGG 0.582519 8.2:+60579901 None:intergenic
TCCTGCCATGGCCAAACATG+TGG 0.582611 8.2:+60579765 None:intergenic
GCATGTGTCGAACCCTCCTA+GGG 0.582815 8.2:+60579891 None:intergenic
CTACCTGTGACAACCCTAGG+AGG 0.586431 8.2:-60579904 MS.gene61081:CDS
ACGCGCTTCCACCGCAAGTG+GGG 0.589037 8.2:+60581395 None:intergenic
ACACAGCCAAAAGTGTACCT+TGG 0.590538 8.2:+60581289 None:intergenic
CAACCAACACAACCAGAACA+AGG 0.590941 8.2:+60583206 None:intergenic
TTGAACGCCAGCGCAGCCGG+AGG 0.592132 8.2:+60580986 None:intergenic
TAGCACCACATGTTTGGCCA+TGG 0.594751 8.2:-60579770 MS.gene61081:CDS
AAAGCCAAAAGCAAAGTTTG+AGG 0.597951 8.2:+60583257 None:intergenic
GAAAATCGTCGATATCCCTC+CGG 0.600442 8.2:-60581002 MS.gene61081:CDS
AGGCAAACAGTAAGAGAATG+TGG 0.601674 8.2:+60581168 None:intergenic
ACACGCGCTTCCACCGCAAG+TGG 0.606518 8.2:+60581393 None:intergenic
TGAACGCCAGCGCAGCCGGA+GGG 0.607788 8.2:+60580987 None:intergenic
ATCCTCATACATAGCACCGC+CGG 0.613653 8.2:-60579793 MS.gene61081:CDS
ATGGTTTGAATGGAGAACAT+TGG 0.614704 8.2:+60583450 None:intergenic
TGGTAGGGTTACATTCATGC+CGG 0.624318 8.2:+60579825 None:intergenic
GTACCTTGGAATTGGGTCAG+TGG 0.625290 8.2:+60581303 None:intergenic
GAGAACAAGAATGTTATCGT+TGG 0.633329 8.2:+60579847 None:intergenic
GTACCCAACCCCACTTGCGG+TGG 0.634233 8.2:-60581403 MS.gene61081:CDS
CAGGTACCCAACCCCACTTG+CGG 0.634918 8.2:-60581406 MS.gene61081:CDS
CTTGGACTTGGGAGATGATG+AGG 0.637478 8.2:+60583537 None:intergenic
TACCGGCGGTGCTATGTATG+AGG 0.638539 8.2:+60579791 None:intergenic
GCCAAAAGCAAAGTTTGAGG+TGG 0.640074 8.2:+60583260 None:intergenic
CAGTTGTACCAATTGCATCA+GGG 0.640171 8.2:-60583334 MS.gene61081:CDS
TACCTGTGACAACCCTAGGA+GGG 0.643334 8.2:-60579903 MS.gene61081:CDS
TAGCCACTGACCCAATTCCA+AGG 0.643632 8.2:-60581306 MS.gene61081:CDS
AGCACCCGATAACGTGAACT+CGG 0.644320 8.2:-60579743 MS.gene61081:CDS
GCGAAACTCGTTTCTTACGG+CGG 0.651651 8.2:+60579945 None:intergenic
GGAAGTTCAGCACCACAACA+GGG 0.652373 8.2:-60581253 MS.gene61081:CDS
GGCCGGTGTATCAGCCCAAG+AGG 0.653863 8.2:-60581105 MS.gene61081:CDS
TCTAGGGTTTCTGAGAAGTG+GGG 0.654621 8.2:+60581072 None:intergenic
AAGCCCAATAGACCCTGTTG+TGG 0.656487 8.2:+60581241 None:intergenic
TGTATGAGGATGTTGTCTGG+TGG 0.659104 8.2:+60579805 None:intergenic
CAAACATGTGGTGCTACCGG+CGG 0.675349 8.2:+60579777 None:intergenic
TGGGCACATCAAATAGCACA+CGG 0.733939 8.2:+60579683 None:intergenic
TTACTAACCTGCTTGCACTG+AGG 0.771908 8.2:+60583119 None:intergenic
GCACATCAAATAGCACACGG+TGG 0.801608 8.2:+60579686 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATACTAATTTGTATTTA+AGG - chr8.2:60583143-60583162 MS.gene61081:CDS 10.0%
!!! ATATTGTTCAAATTTTTTTA+TGG + chr8.2:60581029-60581048 None:intergenic 10.0%
!! AAAATGTCAATTTCAAAAAA+AGG + chr8.2:60580190-60580209 None:intergenic 15.0%
!! AACAATAGTAATTAGTTAAA+TGG - chr8.2:60583060-60583079 MS.gene61081:intron 15.0%
!! TAGATGTATAATTTCTTAAT+TGG + chr8.2:60581516-60581535 None:intergenic 15.0%
!! TGTTTCTTTAATGAAAAAAA+TGG + chr8.2:60582950-60582969 None:intergenic 15.0%
!! TTAGAAAATTTAAACCTAAT+TGG - chr8.2:60580956-60580975 MS.gene61081:CDS 15.0%
!!! ATTTTTTTCATTAAAGAAAC+AGG - chr8.2:60582949-60582968 MS.gene61081:intron 15.0%
!!! TGTCTAAAAATTTAAGATTT+TGG + chr8.2:60581060-60581079 None:intergenic 15.0%
!!! TTTTGAAATTGACATTTTTA+TGG - chr8.2:60580192-60580211 MS.gene61081:intron 15.0%
!!! TTTTTGTTAATATTTCGTTA+TGG + chr8.2:60580829-60580848 None:intergenic 15.0%
!! AATTACTATTGTTATATCCT+TGG + chr8.2:60583053-60583072 None:intergenic 20.0%
!! ACTAACATATCAAATAACTT+TGG - chr8.2:60582469-60582488 MS.gene61081:intron 20.0%
!! ATATTACAAAGTCATACATA+TGG + chr8.2:60582827-60582846 None:intergenic 20.0%
!! TACATATACACTTTATTAGA+AGG + chr8.2:60580342-60580361 None:intergenic 20.0%
!! TATATATACCTTCAAAACAT+GGG - chr8.2:60582668-60582687 MS.gene61081:intron 20.0%
!! TCTCAAACAAAAAAAAACTT+TGG - chr8.2:60582416-60582435 MS.gene61081:intron 20.0%
!! TTAAATGGTTTATACAATGT+CGG - chr8.2:60583075-60583094 MS.gene61081:intron 20.0%
!! TTATATATACCTTCAAAACA+TGG - chr8.2:60582667-60582686 MS.gene61081:intron 20.0%
!! TTTACCAAAATTATCCAATT+AGG + chr8.2:60580973-60580992 None:intergenic 20.0%
!!! AAATAGCTTTTGGTTTTTTT+GGG + chr8.2:60581722-60581741 None:intergenic 20.0%
!!! ATAGTTGAAAAGTTGATATA+TGG - chr8.2:60580851-60580870 MS.gene61081:intron 20.0%
!!! ATCATTGTTATTTTCAACTT+TGG - chr8.2:60582380-60582399 MS.gene61081:intron 20.0%
!!! ATTTGATAATGAACCAAATT+TGG - chr8.2:60580732-60580751 MS.gene61081:intron 20.0%
!!! CAAAAAGTACAAAACATAAA+CGG - chr8.2:60581762-60581781 MS.gene61081:intron 20.0%
!!! GAAAAGTAATAAATAGCTTT+TGG + chr8.2:60581732-60581751 None:intergenic 20.0%
!!! TAAACCTAATTGGATAATTT+TGG - chr8.2:60580966-60580985 MS.gene61081:CDS 20.0%
!!! TAAATAGCTTTTGGTTTTTT+TGG + chr8.2:60581723-60581742 None:intergenic 20.0%
!!! TGTTCAAATTTTTTTATGGT+TGG + chr8.2:60581025-60581044 None:intergenic 20.0%
!!! TTAATTTGTTTTCTTGCTAT+AGG + chr8.2:60581298-60581317 None:intergenic 20.0%
!!! TTTATAGTTTTGAAAAGTGT+CGG + chr8.2:60581321-60581340 None:intergenic 20.0%
!!! TTTATGTTTTGTACTTTTTG+TGG + chr8.2:60581762-60581781 None:intergenic 20.0%
!!! TTTGTTTTGTAAAAAAGTGT+CGG + chr8.2:60581408-60581427 None:intergenic 20.0%
! AAACACACATGCATTATTAT+TGG + chr8.2:60582540-60582559 None:intergenic 25.0%
! AAATACACAAGATAAAACCT+AGG + chr8.2:60582631-60582650 None:intergenic 25.0%
! AATACACAAGATAAAACCTA+GGG + chr8.2:60582630-60582649 None:intergenic 25.0%
! ATATATACCTTCAAAACATG+GGG - chr8.2:60582669-60582688 MS.gene61081:intron 25.0%
! ATCACTCTATTATGTTAACT+TGG - chr8.2:60582176-60582195 MS.gene61081:intron 25.0%
! ATTAGTACCTGAATCAAAAA+TGG + chr8.2:60582280-60582299 None:intergenic 25.0%
! CATTATACATCAACCAAATT+TGG + chr8.2:60580748-60580767 None:intergenic 25.0%
! CTACTCTTACATGAAATATA+AGG + chr8.2:60580917-60580936 None:intergenic 25.0%
! TAATGAAAAAAATGGATGTG+CGG + chr8.2:60582942-60582961 None:intergenic 25.0%
! TGTTAATATTTCGTTATGGA+GGG + chr8.2:60580825-60580844 None:intergenic 25.0%
! TTATTCTAACTATGTTACCA+AGG - chr8.2:60583033-60583052 MS.gene61081:intron 25.0%
! TTGTTAATATTTCGTTATGG+AGG + chr8.2:60580826-60580845 None:intergenic 25.0%
!! AAAACTTTGGACATGTATTT+TGG - chr8.2:60582429-60582448 MS.gene61081:intron 25.0%
!! AATTGACATTTTTATGGCAT+TGG - chr8.2:60580198-60580217 MS.gene61081:intron 25.0%
!! TATAAGCACCCTTTTATTAT+AGG - chr8.2:60581199-60581218 MS.gene61081:CDS 25.0%
!! TCAAACATGTGATATTTTTC+AGG - chr8.2:60581795-60581814 MS.gene61081:intron 25.0%
!! TTTTCTTGTTGAATATTACC+AGG - chr8.2:60583217-60583236 MS.gene61081:CDS 25.0%
!!! AATAGCTTTTGGTTTTTTTG+GGG + chr8.2:60581721-60581740 None:intergenic 25.0%
!!! ATAAAAGGGTGCTTATATAT+AGG + chr8.2:60581196-60581215 None:intergenic 25.0%
!!! ATGAATTAACTCTTTTTTGG+TGG + chr8.2:60580603-60580622 None:intergenic 25.0%
!!! GATAATTTTGGTAAAGACTA+GGG - chr8.2:60580978-60580997 MS.gene61081:CDS 25.0%
!!! GATTATTAGTTTCTTTTCTC+TGG - chr8.2:60580493-60580512 MS.gene61081:intron 25.0%
!!! GTGATGAATTAACTCTTTTT+TGG + chr8.2:60580606-60580625 None:intergenic 25.0%
!!! TAAAAGGGTGCTTATATATA+GGG + chr8.2:60581195-60581214 None:intergenic 25.0%
AACAATACCAATTCTTGAGT+TGG + chr8.2:60583560-60583579 None:intergenic 30.0%
AAGTCCAACATACATTGTTA+AGG - chr8.2:60579923-60579942 MS.gene61081:CDS 30.0%
AATAGAGTGATGATCTTCTA+GGG + chr8.2:60582167-60582186 None:intergenic 30.0%
ACAATACCAATTCTTGAGTT+GGG + chr8.2:60583559-60583578 None:intergenic 30.0%
ACCCTTTGCCTATAATAAAA+GGG + chr8.2:60581210-60581229 None:intergenic 30.0%
ACGTAATCTTGATCTCAAAA+TGG + chr8.2:60582894-60582913 None:intergenic 30.0%
ACTAACATTCCTAAAACTTG+TGG + chr8.2:60580072-60580091 None:intergenic 30.0%
ATTCTCACGAAAAAACTAAG+TGG - chr8.2:60582722-60582741 MS.gene61081:intron 30.0%
TAATAGAGTGATGATCTTCT+AGG + chr8.2:60582168-60582187 None:intergenic 30.0%
TAATCTTGATCTCAAAATGG+TGG + chr8.2:60582891-60582910 None:intergenic 30.0%
TACATTGTTAAGGCTAAGTT+TGG - chr8.2:60579933-60579952 MS.gene61081:CDS 30.0%
TATTATGCTTGACCAACAAA+GGG - chr8.2:60582568-60582587 MS.gene61081:intron 30.0%
TCAATACTTACCTTTCCTAT+AGG - chr8.2:60581676-60581695 MS.gene61081:intron 30.0%
TGCAGATAGTATTATGTCTT+TGG + chr8.2:60580628-60580647 None:intergenic 30.0%
TTATCGTTGGTACAACTATT+GGG + chr8.2:60583363-60583382 None:intergenic 30.0%
TTCACTTAAGCTTATGCTAT+TGG - chr8.2:60580456-60580475 MS.gene61081:intron 30.0%
TTCTCACGAAAAAACTAAGT+GGG - chr8.2:60582723-60582742 MS.gene61081:intron 30.0%
! AACTTGGTTGCTATTAAAGT+TGG - chr8.2:60582192-60582211 MS.gene61081:intron 30.0%
! AATTTTGGATCTTGCTAACA+AGG - chr8.2:60581473-60581492 MS.gene61081:intron 30.0%
! ACCCTTTTATTATAGGCAAA+GGG - chr8.2:60581206-60581225 MS.gene61081:CDS 30.0%
!! AGGAAAGGTGTTAGAAAAAT+TGG + chr8.2:60579657-60579676 None:intergenic 30.0%
!! GGAAAGGTGTTAGAAAAATT+GGG + chr8.2:60579656-60579675 None:intergenic 30.0%
!! GGATAATTTTGGTAAAGACT+AGG - chr8.2:60580977-60580996 MS.gene61081:CDS 30.0%
!! TTAGCCTTAACAATGTATGT+TGG + chr8.2:60579930-60579949 None:intergenic 30.0%
!! TTTTATGGCATTGGAATGTT+TGG - chr8.2:60580207-60580226 MS.gene61081:intron 30.0%
!!! AGTTTTAGGAATGTTAGTTG+TGG - chr8.2:60580074-60580093 MS.gene61081:intron 30.0%
!!! ATCTTTTTTGCCTATAGGAA+AGG + chr8.2:60581689-60581708 None:intergenic 30.0%
!!! ATTGGTTTTGATGGTTTGAA+TGG + chr8.2:60579783-60579802 None:intergenic 30.0%
!!! TAAGCTGTTTTTGAAAGTGT+CGG + chr8.2:60581354-60581373 None:intergenic 30.0%
AAAGCCAAAAGCAAAGTTTG+AGG + chr8.2:60579966-60579985 None:intergenic 35.0%
AAATGTGACATCCAAGATGA+TGG - chr8.2:60579717-60579736 MS.gene61081:CDS 35.0%
AACCATCAAAACCAATGTCA+TGG - chr8.2:60579787-60579806 MS.gene61081:CDS 35.0%
ACCATCAAAACCAATGTCAT+GGG - chr8.2:60579788-60579807 MS.gene61081:CDS 35.0%
ACGATAACATTCTTGTTCTC+CGG - chr8.2:60583376-60583395 MS.gene61081:CDS 35.0%
ATGGTTTGAATGGAGAACAT+TGG + chr8.2:60579773-60579792 None:intergenic 35.0%
CACCCTTTGCCTATAATAAA+AGG + chr8.2:60581211-60581230 None:intergenic 35.0%
CGTGTGTGATGACAAAATTA+AGG - chr8.2:60582759-60582778 MS.gene61081:intron 35.0%
CTAAAACTTGTGGACTTAAC+AGG + chr8.2:60580062-60580081 None:intergenic 35.0%
CTATTATGCTTGACCAACAA+AGG - chr8.2:60582567-60582586 MS.gene61081:intron 35.0%
GAGAACAAGAATGTTATCGT+TGG + chr8.2:60583376-60583395 None:intergenic 35.0%
GTTATCGTTGGTACAACTAT+TGG + chr8.2:60583364-60583383 None:intergenic 35.0%
TCATACGATTAATCTTCCCT+AGG - chr8.2:60582611-60582630 MS.gene61081:intron 35.0%
TGTGCATTCAACTTCACTTA+TGG - chr8.2:60581847-60581866 MS.gene61081:intron 35.0%
TTAAGTCGCCTAACTTTAGT+GGG - chr8.2:60582079-60582098 MS.gene61081:intron 35.0%
TTGATCTCAAAATGGTGGTT+GGG + chr8.2:60582886-60582905 None:intergenic 35.0%
TTTAAGTCGCCTAACTTTAG+TGG - chr8.2:60582078-60582097 MS.gene61081:intron 35.0%
! ACAAGTATTGCATTCTAGCT+TGG + chr8.2:60579845-60579864 None:intergenic 35.0%
! AGGGTGCTTATATATAAGGA+CGG - chr8.2:60581225-60581244 MS.gene61081:CDS 35.0%
! ATCTTGGATGTCACATTTTG+GGG + chr8.2:60579715-60579734 None:intergenic 35.0%
! ATGTGGACTTGTAGAGATTT+TGG + chr8.2:60582038-60582057 None:intergenic 35.0%
! CAAAAGTGTACCTTGGAATT+GGG + chr8.2:60581927-60581946 None:intergenic 35.0%
! CACCCTTTTATTATAGGCAA+AGG - chr8.2:60581205-60581224 MS.gene61081:CDS 35.0%
! CATCTTGGATGTCACATTTT+GGG + chr8.2:60579716-60579735 None:intergenic 35.0%
! CTGTTAAGTCCACAAGTTTT+AGG - chr8.2:60580060-60580079 MS.gene61081:intron 35.0%
! TCATCTTGGATGTCACATTT+TGG + chr8.2:60579717-60579736 None:intergenic 35.0%
!! GCAAAGGGTGCTTATATATA+AGG - chr8.2:60581221-60581240 MS.gene61081:CDS 35.0%
!! TGCTTTTTGTACAAGACATG+TGG + chr8.2:60582863-60582882 None:intergenic 35.0%
!!! ACTTGTAGAGATTTTGGCTT+TGG + chr8.2:60582032-60582051 None:intergenic 35.0%
!!! CTTGTAGAGATTTTGGCTTT+GGG + chr8.2:60582031-60582050 None:intergenic 35.0%
!!! TCAAACTTTGCTTTTGGCTT+TGG - chr8.2:60579965-60579984 MS.gene61081:CDS 35.0%
!!! TCGTGATCTTTTTTGCCTAT+AGG + chr8.2:60581694-60581713 None:intergenic 35.0%
AAGTTAGGCGACTTAAAGCT+AGG + chr8.2:60582075-60582094 None:intergenic 40.0%
ACACTTTCTTCCCATGACAT+TGG + chr8.2:60579801-60579820 None:intergenic 40.0%
AGACTAGGGTAGATTCACAT+TGG - chr8.2:60580992-60581011 MS.gene61081:CDS 40.0%
AGGCAAACAGTAAGAGAATG+TGG + chr8.2:60582055-60582074 None:intergenic 40.0%
ATACTAGTAGTGATGCTGCT+TGG - chr8.2:60579988-60580007 MS.gene61081:intron 40.0%
ATCAAAAATGGTTCCAGCAC+CGG + chr8.2:60582268-60582287 None:intergenic 40.0%
ATTTGTCTCCCTGATGCAAT+TGG + chr8.2:60579897-60579916 None:intergenic 40.0%
CAGTTGTACCAATTGCATCA+GGG - chr8.2:60579886-60579905 MS.gene61081:CDS 40.0%
CTATGTATGAGGATGTTGTC+TGG + chr8.2:60583421-60583440 None:intergenic 40.0%
CTTGATCTCAAAATGGTGGT+TGG + chr8.2:60582887-60582906 None:intergenic 40.0%
GACTAGGGTAGATTCACATT+GGG - chr8.2:60580993-60581012 MS.gene61081:CDS 40.0%
GGCTGTGTTAACAAAACAAC+AGG - chr8.2:60581946-60581965 MS.gene61081:intron 40.0%
TCAAAATCCTACACGTGACT+TGG - chr8.2:60582801-60582820 MS.gene61081:intron 40.0%
TCAGTTGTACCAATTGCATC+AGG - chr8.2:60579885-60579904 MS.gene61081:CDS 40.0%
TGATTTCCTAGCAACTAAGC+TGG + chr8.2:60579868-60579887 None:intergenic 40.0%
TGCTTATATATAGGGACGGA+GGG + chr8.2:60581187-60581206 None:intergenic 40.0%
! ACCACCTCAAACTTTGCTTT+TGG - chr8.2:60579959-60579978 MS.gene61081:CDS 40.0%
! AGGGTGCTTATATATAGGGA+CGG + chr8.2:60581191-60581210 None:intergenic 40.0%
! ATTCCTTGTTCTGGTTGTGT+TGG - chr8.2:60580011-60580030 MS.gene61081:intron 40.0%
! CCAAAAGTGTACCTTGGAAT+TGG + chr8.2:60581928-60581947 None:intergenic 40.0%
! CCAATTCCAAGGTACACTTT+TGG - chr8.2:60581925-60581944 MS.gene61081:intron 40.0%
! CTCAACACTACTTGTTAGCT+TGG - chr8.2:60580132-60580151 MS.gene61081:intron 40.0%
! GAGAACATTGGCTGAAGATA+TGG + chr8.2:60579761-60579780 None:intergenic 40.0%
! TGGTTGCTATTAAAGTTGGC+CGG - chr8.2:60582196-60582215 MS.gene61081:intron 40.0%
!! GCTGGAACCATTTTTGATTC+AGG - chr8.2:60582270-60582289 MS.gene61081:intron 40.0%
!! GGAAAGGTAAGTATTGACGA+TGG + chr8.2:60581673-60581692 None:intergenic 40.0%
!! TTCTAGGGTTTCTGAGAAGT+GGG + chr8.2:60582152-60582171 None:intergenic 40.0%
!!! GTGGTTTTGTTGTTGCATGT+TGG + chr8.2:60583518-60583537 None:intergenic 40.0%
!!! TCCCATGACATTGGTTTTGA+TGG + chr8.2:60579792-60579811 None:intergenic 40.0%
AAACTACCTGTGACAACCCT+AGG - chr8.2:60583313-60583332 MS.gene61081:CDS 45.0%
AACACCGAGTTCACGTTATC+GGG + chr8.2:60583484-60583503 None:intergenic 45.0%
ACTTGTCCAGCTTAGTTGCT+AGG - chr8.2:60579859-60579878 MS.gene61081:CDS 45.0%
AGTCGAGTGAATACAGTACC+TGG + chr8.2:60583238-60583257 None:intergenic 45.0%
ATGATGAGGCAGAGAAGGAA+AGG + chr8.2:60579672-60579691 None:intergenic 45.0%
CAACACCGAGTTCACGTTAT+CGG + chr8.2:60583485-60583504 None:intergenic 45.0%
CAACCAACACAACCAGAACA+AGG + chr8.2:60580017-60580036 None:intergenic 45.0%
CACCTACTAGTTCCCTTTGT+TGG + chr8.2:60582583-60582602 None:intergenic 45.0%
CTCAACGACCCACTAAAGTT+AGG + chr8.2:60582090-60582109 None:intergenic 45.0%
GAAAATCGTCGATATCCCTC+CGG - chr8.2:60582218-60582237 MS.gene61081:intron 45.0%
GACCAACAAAGGGAACTAGT+AGG - chr8.2:60582578-60582597 MS.gene61081:intron 45.0%
GATGTGCCCAACTCAAGAAT+TGG - chr8.2:60583550-60583569 MS.gene61081:CDS 45.0%
GCCAAAAGCAAAGTTTGAGG+TGG + chr8.2:60579963-60579982 None:intergenic 45.0%
GGGGTATACTTGATCCTCTT+GGG + chr8.2:60582132-60582151 None:intergenic 45.0%
GTGCTTATATATAGGGACGG+AGG + chr8.2:60581188-60581207 None:intergenic 45.0%
GTGGACTTAACAGGAGCAAA+AGG + chr8.2:60580053-60580072 None:intergenic 45.0%
TCGGCGAAACTCGTTTCTTA+CGG + chr8.2:60583281-60583300 None:intergenic 45.0%
TGGGCACATCAAATAGCACA+CGG + chr8.2:60583540-60583559 None:intergenic 45.0%
TGGGGTATACTTGATCCTCT+TGG + chr8.2:60582133-60582152 None:intergenic 45.0%
TGGTAGGGTTACATTCATGC+CGG + chr8.2:60583398-60583417 None:intergenic 45.0%
TGTATGAGGATGTTGTCTGG+TGG + chr8.2:60583418-60583437 None:intergenic 45.0%
TGTCCTACAGCTAGAAGCTA+AGG - chr8.2:60579818-60579837 MS.gene61081:CDS 45.0%
TTACTAACCTGCTTGCACTG+AGG + chr8.2:60580104-60580123 None:intergenic 45.0%
TTCTTCCATAGCAGCTAGTG+TGG - chr8.2:60581873-60581892 MS.gene61081:intron 45.0%
TTTCTTACGGCGGTGTATAC+AGG + chr8.2:60583268-60583287 None:intergenic 45.0%
! ACACAGCCAAAAGTGTACCT+TGG + chr8.2:60581934-60581953 None:intergenic 45.0%
! AGGGTTGTCACAGGTAGTTT+TGG + chr8.2:60583313-60583332 None:intergenic 45.0%
! GAGGGATATCGACGATTTTC+CGG + chr8.2:60582218-60582237 None:intergenic 45.0%
! TCTAGGGTTTCTGAGAAGTG+GGG + chr8.2:60582151-60582170 None:intergenic 45.0%
! TTAGTGGGTCGTTGAGACTT+GGG - chr8.2:60582094-60582113 MS.gene61081:intron 45.0%
! TTTAGTGGGTCGTTGAGACT+TGG - chr8.2:60582093-60582112 MS.gene61081:intron 45.0%
!! CTTCTAGGGTTTCTGAGAAG+TGG + chr8.2:60582153-60582172 None:intergenic 45.0%
!! GATTGTGCCCCATGTTTTGA+AGG + chr8.2:60582679-60582698 None:intergenic 45.0%
!! TGAGTGTTGAGCCATCATCT+TGG + chr8.2:60579731-60579750 None:intergenic 45.0%
!!! CACATTTTGGGGTTTGAGCT+TGG + chr8.2:60579704-60579723 None:intergenic 45.0%
!!! GTAGTTTTGGACCAACTCGT+CGG + chr8.2:60583300-60583319 None:intergenic 45.0%
!! TATATAAATATAAAATAGAA+AGG + chr8.2:60582654-60582673 None:intergenic 5.0%
!!! AATTAAACATTTATAAATTT+TGG - chr8.2:60581458-60581477 MS.gene61081:intron 5.0%
AAGCCCAATAGACCCTGTTG+TGG + chr8.2:60581982-60582001 None:intergenic 50.0%
ACAACAGGGTCTATTGGGCT+TGG - chr8.2:60581981-60582000 MS.gene61081:intron 50.0%
AGCACCACAACAGGGTCTAT+TGG - chr8.2:60581975-60581994 MS.gene61081:intron 50.0%
AGCACCCGATAACGTGAACT+CGG - chr8.2:60583477-60583496 MS.gene61081:CDS 50.0%
AGGAAGTTCAGCACCACAAC+AGG - chr8.2:60581966-60581985 MS.gene61081:intron 50.0%
ATCCTCATACATAGCACCGC+CGG - chr8.2:60583427-60583446 MS.gene61081:CDS 50.0%
ATCCTCTTGGGCTGATACAC+CGG + chr8.2:60582120-60582139 None:intergenic 50.0%
CAACAGGGTCTATTGGGCTT+GGG - chr8.2:60581982-60582001 MS.gene61081:intron 50.0%
CATATGGCCAAGTCACGTGT+AGG + chr8.2:60582811-60582830 None:intergenic 50.0%
CTTGGACTTGGGAGATGATG+AGG + chr8.2:60579686-60579705 None:intergenic 50.0%
GAGGATGTTGTCTGGTGGTA+GGG + chr8.2:60583413-60583432 None:intergenic 50.0%
GCACATCAAATAGCACACGG+TGG + chr8.2:60583537-60583556 None:intergenic 50.0%
GCACCACAACAGGGTCTATT+GGG - chr8.2:60581976-60581995 MS.gene61081:intron 50.0%
GCGAAACTCGTTTCTTACGG+CGG + chr8.2:60583278-60583297 None:intergenic 50.0%
GCTGCTTGGATTCCTTGTTC+TGG - chr8.2:60580002-60580021 MS.gene61081:intron 50.0%
GGAAGTTCAGCACCACAACA+GGG - chr8.2:60581967-60581986 MS.gene61081:intron 50.0%
GTACCTTGGAATTGGGTCAG+TGG + chr8.2:60581920-60581939 None:intergenic 50.0%
TACCTGTGACAACCCTAGGA+GGG - chr8.2:60583317-60583336 MS.gene61081:CDS 50.0%
TAGCACCACATGTTTGGCCA+TGG - chr8.2:60583450-60583469 MS.gene61081:CDS 50.0%
TAGCCACTGACCCAATTCCA+AGG - chr8.2:60581914-60581933 MS.gene61081:intron 50.0%
TGAGGATGTTGTCTGGTGGT+AGG + chr8.2:60583414-60583433 None:intergenic 50.0%
TGGAATTGGGTCAGTGGCTA+GGG + chr8.2:60581914-60581933 None:intergenic 50.0%
TGGCTAGGGTAAGTGTGTCT+TGG + chr8.2:60581900-60581919 None:intergenic 50.0%
TGGGGTTTGAGCTTGGACTT+GGG + chr8.2:60579697-60579716 None:intergenic 50.0%
TTGGAATTGGGTCAGTGGCT+AGG + chr8.2:60581915-60581934 None:intergenic 50.0%
TTGGACCACACTAGCTGCTA+TGG + chr8.2:60581881-60581900 None:intergenic 50.0%
TTGGGGTTTGAGCTTGGACT+TGG + chr8.2:60579698-60579717 None:intergenic 50.0%
TTTGGGCCAAGAGCGACAAT+GGG + chr8.2:60582014-60582033 None:intergenic 50.0%
! GGTCCTTAGCTTCTAGCTGT+AGG + chr8.2:60579824-60579843 None:intergenic 50.0%
AACCCTCCTAGGGTTGTCAC+AGG + chr8.2:60583322-60583341 None:intergenic 55.0%
AACGAGTTTCGCCGACGAGT+TGG - chr8.2:60583286-60583305 MS.gene61081:CDS 55.0%
ACCACATGTTTGGCCATGGC+AGG - chr8.2:60583454-60583473 MS.gene61081:CDS 55.0%
AGCATGTGTCGAACCCTCCT+AGG + chr8.2:60583333-60583352 None:intergenic 55.0%
CTACCTGTGACAACCCTAGG+AGG - chr8.2:60583316-60583335 MS.gene61081:CDS 55.0%
CTGGCGTTCAACCCAACTAC+CGG - chr8.2:60582246-60582265 MS.gene61081:intron 55.0%
CTTTGGGCCAAGAGCGACAA+TGG + chr8.2:60582015-60582034 None:intergenic 55.0%
GCATGTGTCGAACCCTCCTA+GGG + chr8.2:60583332-60583351 None:intergenic 55.0%
GGGAGATGATGAGGCAGAGA+AGG + chr8.2:60579677-60579696 None:intergenic 55.0%
TCCTGCCATGGCCAAACATG+TGG + chr8.2:60583458-60583477 None:intergenic 55.0%
TGGTTCTCCTCAGTGCAAGC+AGG - chr8.2:60580094-60580113 MS.gene61081:intron 55.0%
TTCAACCCAACTACCGGTGC+TGG - chr8.2:60582252-60582271 MS.gene61081:intron 55.0%
!! ATGGTTCCAGCACCGGTAGT+TGG + chr8.2:60582261-60582280 None:intergenic 55.0%
!! CAAACATGTGGTGCTACCGG+CGG + chr8.2:60583446-60583465 None:intergenic 55.0%
!! GGCCAAACATGTGGTGCTAC+CGG + chr8.2:60583449-60583468 None:intergenic 55.0%
!! TACCGGCGGTGCTATGTATG+AGG + chr8.2:60583432-60583451 None:intergenic 55.0%
!! TGGTTCCAGCACCGGTAGTT+GGG + chr8.2:60582260-60582279 None:intergenic 55.0%
CAGGTACCCAACCCCACTTG+CGG - chr8.2:60581814-60581833 MS.gene61081:intron 60.0%
CCAAGAGCGACAATGGGCCT+CGG + chr8.2:60582008-60582027 None:intergenic 60.0%
CGCCGGTAGCACCACATGTT+TGG - chr8.2:60583444-60583463 MS.gene61081:CDS 60.0%
CTTCCACCGCAAGTGGGGTT+GGG + chr8.2:60581823-60581842 None:intergenic 60.0%
GTTATCGGGTGCTCCTGCCA+TGG + chr8.2:60583470-60583489 None:intergenic 60.0%
TGGGTCGTTGAGACTTGGGC+CGG - chr8.2:60582098-60582117 MS.gene61081:intron 60.0%
ACACGCGCTTCCACCGCAAG+TGG + chr8.2:60581830-60581849 None:intergenic 65.0%
ACGCGCTTCCACCGCAAGTG+GGG + chr8.2:60581828-60581847 None:intergenic 65.0%
CACGCGCTTCCACCGCAAGT+GGG + chr8.2:60581829-60581848 None:intergenic 65.0%
CCGAGGCCCATTGTCGCTCT+TGG - chr8.2:60582005-60582024 MS.gene61081:intron 65.0%
GCTTCCACCGCAAGTGGGGT+TGG + chr8.2:60581824-60581843 None:intergenic 65.0%
GGCCGGTGTATCAGCCCAAG+AGG - chr8.2:60582115-60582134 MS.gene61081:intron 65.0%
GGTCTATTGGGCTTGGGCCG+AGG - chr8.2:60581988-60582007 MS.gene61081:intron 65.0%
GTACCCAACCCCACTTGCGG+TGG - chr8.2:60581817-60581836 MS.gene61081:intron 65.0%
CGATATCCCTCCGGCTGCGC+TGG - chr8.2:60582227-60582246 MS.gene61081:intron 70.0%
GGGTTGAACGCCAGCGCAGC+CGG + chr8.2:60582240-60582259 None:intergenic 70.0%
TGAACGCCAGCGCAGCCGGA+GGG + chr8.2:60582236-60582255 None:intergenic 70.0%
TTGAACGCCAGCGCAGCCGG+AGG + chr8.2:60582237-60582256 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 60579645 60583597 60579645 ID=MS.gene61081
chr8.2 mRNA 60579645 60583597 60579645 ID=MS.gene61081.t1;Parent=MS.gene61081
chr8.2 exon 60583127 60583597 60583127 ID=MS.gene61081.t1.exon1;Parent=MS.gene61081.t1
chr8.2 CDS 60583127 60583597 60583127 ID=cds.MS.gene61081.t1;Parent=MS.gene61081.t1
chr8.2 exon 60580951 60581425 60580951 ID=MS.gene61081.t1.exon2;Parent=MS.gene61081.t1
chr8.2 CDS 60580951 60581425 60580951 ID=cds.MS.gene61081.t1;Parent=MS.gene61081.t1
chr8.2 exon 60579645 60580003 60579645 ID=MS.gene61081.t1.exon3;Parent=MS.gene61081.t1
chr8.2 CDS 60579645 60580003 60579645 ID=cds.MS.gene61081.t1;Parent=MS.gene61081.t1
Gene Sequence

>MS.gene61081

ATGAAAATCCCAATTTTTCTAACACCTTTCCTTCTCTGCCTCATCATCTCCCAAGTCCAAGCTCAAACCCCAAAATGTGACATCCAAGATGATGGCTCAACACTCAAAGTGTTCCATATCTTCAGCCAATGTTCTCCATTCAAACCATCAAAACCAATGTCATGGGAAGAAAGTGTCCTACAGCTAGAAGCTAAGGACCAAGCTAGAATGCAATACTTGTCCAGCTTAGTTGCTAGGAAATCAGTTGTACCAATTGCATCAGGGAGACAAATCATACAAAGTCCAACATACATTGTTAAGGCTAAGTTTGGTACACCACCTCAAACTTTGCTTTTGGCTTTGGATACTAGTAGTGATGCTGCTTGGATTCCTTGTTCTGGTTGTGTTGGTTGTTCAACATCAAAACCTTTTGCTCCTGTTAAGTCCACAAGTTTTAGGAATGTTAGTTGTGGTTCTCCTCAGTGCAAGCAGGTTAGTAATTAACATACTCAACACTACTTGTTAGCTTGGATTTTTTTCTTGCATCTTTTTTAGTTCTTTTACCTTTTTTTGAAATTGACATTTTTATGGCATTGGAATGTTTGGATCTCATTGTTTTGATTATATAATATGATATTGATTGTGACTAGTATATATGTGTGTGTCTATATATAATATGTGTGTCTCTCATTATTTAGAAAAGTATTTAATTTGTCCTTCTAATAAAGTGTATATGTACTAGTAATATCTAGTGATTTATTTTAACTTAATTTGATCTGAAGTGTATAATGTTGACAATGTTGAAACTTATTAAAATTTAAGAGTGAAATTATTCACTTAAGCTTATGCTATTGGTAAAATTACTTTAAGATTATTAGTTTCTTTTCTCTGGTTTCAATATTATTTGTTTCTTTTAACTCAAAAAATTATTTGTTTCTCTATCTCATCACACGTAACTACATAATAGTATATCTCACCACCAAAAAAGAGTTAATTCATCACCAAAGACATAATACTATCTGCAAGAGCTCTTGAACAATTTTTTATTTTGTTTCATAAGAAACTCTTAATATGATATATACGTGTGTGTGTGTCAAAATCAACATAAATTTGATAATGAACCAAATTTGGTTGATGTATAATGTGTTGACAGTTGATGAATCAAAATCAAAACAATATACATTTGATAAAATACATACCCTCCATAACGAAATATTAACAAAAATGATAGTTGAAAAGTTGATATATGGATTAAATTTTAGATACGTCAACATTTCAACTCTTGTTTTTCCTTATATTTCATGTAAGAGTAGTATAATATAAAATTCTTTGTTAGAAAATTTAAACCTAATTGGATAATTTTGGTAAAGACTAGGGTAGATTCACATTGGGTTAAAACCAACCATAAAAAAATTTGAACAATATATCTTCATCCAAAATCTTAAATTTTTAGACATACAATCATATTGTTTATACGTTATTCAATATCTATTTATCTTATTTAATGTGTTACTTCTAACTCAACAGATCAAACTATCTATGCATGCACTCAAATATACTCCCTCCGTCCCTATATATAAGCACCCTTTTATTATAGGCAAAGGGTGCTTATATATAAGGACGGAGAGAGTATAATCTATCAGACACTTATAAAAACAGCAGATGCTTACACCTATAGCAAGAAAACAAATTAACCGACACTTTTCAAAACTATAAAAACAAATTAACCGACACTTTCAAAAACAGCTTATTCTTGTATCTATAGCATGAAAACAAATTTACCGACACTTTTTTACAAAACAAAATAACTAACATTCTTCATTTAAATATAATAAATTAAACATTTATAAATTTTGGATCTTGCTAACAAGGTTACTTTTTAATAATTACCAATTAAGAAATTATACATCTAAAAAGTCTAAAACTTTAATCTACGATCAATTAAATACACAAATTCTTATTAAAATTTATTATCTAAATTCGTTAAAGAGTTCTCATAAATTTTTCACTATATTACGTAGTATATTCGAATCATAAAACTTTATTCCATCGTCAATACTTACCTTTCCTATAGGCAAAAAAGATCACGAGATTCCCCAAAAAAACCAAAAGCTATTTATTACTTTTCTATTTTTCCACAAAAAGTACAAAACATAAACGGTTCTCAAATCTCAAACATGTGATATTTTTCAGGTACCCAACCCCACTTGCGGTGGAAGCGCGTGTGCATTCAACTTCACTTATGGTAGTTCTTCCATAGCAGCTAGTGTGGTCCAAGACACACTTACCCTAGCCACTGACCCAATTCCAAGGTACACTTTTGGCTGTGTTAACAAAACAACAGGAAGTTCAGCACCACAACAGGGTCTATTGGGCTTGGGCCGAGGCCCATTGTCGCTCTTGGCCCAAAGCCAAAATCTCTACAAGTCCACATTCTCTTACTGTTTGCCTAGCTTTAAGTCGCCTAACTTTAGTGGGTCGTTGAGACTTGGGCCGGTGTATCAGCCCAAGAGGATCAAGTATACCCCACTTCTCAGAAACCCTAGAAGATCATCACTCTATTATGTTAACTTGGTTGCTATTAAAGTTGGCCGGAAAATCGTCGATATCCCTCCGGCTGCGCTGGCGTTCAACCCAACTACCGGTGCTGGAACCATTTTTGATTCAGGTACTAATGTCTTCATTAACATTTAACATATTCTAATTCTTAACAAAATGAATTCTAGTATAGTTTTACAAATCATAAGTATACATTTATCATTGTTATTTTCAACTTTGGACATGCACTCTTTTCTCAAACAAAAAAAAACTTTGGACATGTATTTTGGATAATGAAATTTCAGATACTAACATATCAAATAACTTTGGACATGTATTGTAAATAAATTTTTTACTACTAATAAATTTAAACTACCAATAATAATGCATGTGTGTTTAAGTTTACTATTATGCTTGACCAACAAAGGGAACTAGTAGGTGTTATGAGATCATACGATTAATCTTCCCTAGGTTTTATCTTGTGTATTTCCTTTCTATTTTATATTTATATATACCTTCAAAACATGGGGCACAATCTTGTGTAGTCATGTTATGTTCACATTCTCACGAAAAAACTAAGTGGGTTTCATGTTTTAACGTGTGTGATGACAAAATTAAGGTCAAGTTGAGACAAATTTGTCAAAATCCTACACGTGACTTGGCCATATGTATGACTTTGTAATATTATGTATCATGCTCCACATGTCTTGTACAAAAAGCACCCAACCACCATTTTGAGATCAAGATTACGTACAAGTACAATTATAATTATAATCACCGCACATCCATTTTTTTCATTAAAGAAACAGGAGAATGATGATTTTGAAAATAAATTGACAATATTGAAGCAAACAACAATAACACTAAGACATTATTCTAACTATGTTACCAAGGATATAACAATAGTAATTAGTTAAATGGTTTATACAATGTCGGTGTAAAAATTCATTTTATACATAAGCATACTTTGTAATCTTTCAAATAATACTAATTTGTATTTAAGGCGCATATTTTATTTTCTCATGTCAATTTCACTAAACATTTTCTAAATCTGATTTTCTTGTTGAATATTACCAGGTACTGTATTCACTCGACTAGCTGAACCTGTATACACCGCCGTAAGAAACGAGTTTCGCCGACGAGTTGGTCCAAAACTACCTGTGACAACCCTAGGAGGGTTCGACACATGCTACAATGTCCCAATAGTTGTACCAACGATAACATTCTTGTTCTCCGGCATGAATGTAACCCTACCACCAGACAACATCCTCATACATAGCACCGCCGGTAGCACCACATGTTTGGCCATGGCAGGAGCACCCGATAACGTGAACTCGGTGTTGAACGTGATAGCCAACATGCAACAACAAAACCACCGTGTGCTATTTGATGTGCCCAACTCAAGAATTGGTATTGTTCGTGAGCTTTGCACCTAA
Protein sequence

>MS.gene61081.t1

MKIPIFLTPFLLCLIISQVQAQTPKCDIQDDGSTLKVFHIFSQCSPFKPSKPMSWEESVLQLEAKDQARMQYLSSLVARKSVVPIASGRQIIQSPTYIVKAKFGTPPQTLLLALDTSSDAAWIPCSGCVGCSTSKPFAPVKSTSFRNVSCGSPQCKQVPNPTCGGSACAFNFTYGSSSIAASVVQDTLTLATDPIPRYTFGCVNKTTGSSAPQQGLLGLGRGPLSLLAQSQNLYKSTFSYCLPSFKSPNFSGSLRLGPVYQPKRIKYTPLLRNPRRSSLYYVNLVAIKVGRKIVDIPPAALAFNPTTGAGTIFDSGTVFTRLAEPVYTAVRNEFRRRVGPKLPVTTLGGFDTCYNVPIVVPTITFLFSGMNVTLPPDNILIHSTAGSTTCLAMAGAPDNVNSVLNVIANMQQQNHRVLFDVPNSRIGIVRELCT