AlfalfaGEDB | MS.gene021696

M. sativa cultivar XinJiangDaYe / MS.gene021696

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF04770.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021696.t1	XP_003621193.1	97.8	232	3	2	1	231	111	341	1.60E-82	315.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021696.t1	Q9LHF0	69.3	176	47	2	1	169	75	250	3.2e-23	110.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021696.t1	A2Q1B8	97.8	232	3	2	1	231	111	341	1.2e-82	315.8

TFs/TRs:

Gene ID	Type	Classification
MS.gene021696.t1	TF	zf-HD

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene021696	MS.gene012789	PPI
MS.gene021696	MS.gene95053	PPI
MS.gene021696	MS.gene43476	PPI
MS.gene042050	MS.gene021696	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021696.t1	MTR_7g010300	97.845	232	3	2	1	231	111	341	8.49e-169	468
MS.gene021696.t1	MTR_6g012280	64.737	190	53	5	1	184	114	295	4.91e-57	184
MS.gene021696.t1	MTR_1g077020	40.805	174	79	5	4	173	33	186	9.41e-36	125
MS.gene021696.t1	MTR_3g100650	43.103	174	73	5	13	173	113	273	1.78e-35	127
MS.gene021696.t1	MTR_5g020510	39.779	181	92	6	4	174	123	296	1.21e-32	120
MS.gene021696.t1	MTR_5g065440	39.785	186	78	5	13	173	91	267	9.11e-32	117
MS.gene021696.t1	MTR_5g032600	40.323	186	79	6	4	173	102	271	5.70e-31	115
MS.gene021696.t1	MTR_4g134580	36.967	211	93	8	4	174	137	347	1.32e-30	116
MS.gene021696.t1	MTR_2g076780	36.517	178	83	5	14	173	71	236	5.69e-27	103
MS.gene021696.t1	MTR_8g467340	42.748	131	55	5	110	229	270	391	5.58e-25	101
MS.gene021696.t1	MTR_8g069080	34.091	176	81	5	14	169	103	263	9.17e-23	93.6
MS.gene021696.t1	MTR_6g073013	63.333	60	21	1	111	169	101	160	1.63e-22	90.9
MS.gene021696.t1	MTR_8g069380	55.224	67	30	0	107	173	48	114	1.94e-20	83.6
MS.gene021696.t1	MTR_8g051480	59.701	67	26	1	107	173	63	128	4.26e-20	83.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021696.t1	AT5G15210	51.913	183	65	6	1	175	79	246	2.42e-46	155
MS.gene021696.t1	AT1G69600	48.333	180	65	5	1	170	54	215	6.03e-46	153
MS.gene021696.t1	AT3G28920	47.525	202	72	6	14	189	88	281	4.42e-40	140
MS.gene021696.t1	AT1G14440	40.526	190	89	5	4	181	114	291	3.16e-35	127
MS.gene021696.t1	AT1G14440	40.526	190	89	5	4	181	114	291	3.16e-35	127
MS.gene021696.t1	AT4G24660	41.176	170	77	3	4	173	73	219	4.45e-35	124
MS.gene021696.t1	AT5G39760	49.198	187	77	5	14	185	92	275	1.45e-34	126
MS.gene021696.t1	AT2G02540	37.629	194	102	5	4	185	111	297	2.28e-34	125
MS.gene021696.t1	AT2G02540	37.629	194	102	5	4	185	111	297	2.28e-34	125
MS.gene021696.t1	AT4G24660	41.317	167	75	3	4	170	73	216	4.70e-34	122
MS.gene021696.t1	AT5G60480	39.881	168	68	5	5	167	64	203	8.72e-32	116
MS.gene021696.t1	AT5G65410	40.237	169	86	4	4	172	99	252	4.12e-30	113
MS.gene021696.t1	AT1G75240	34.634	205	95	3	4	172	100	301	1.39e-28	110
MS.gene021696.t1	AT2G18350	36.810	163	86	3	13	174	115	261	5.27e-27	104
MS.gene021696.t1	AT3G50890	38.953	172	80	7	13	177	93	246	7.61e-23	93.6
MS.gene021696.t1	AT5G42780	32.298	161	89	4	13	170	95	238	7.89e-17	77.0

Find 59 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAATTCTAGAAAGCGTTTC+AGG	0.216225	7.4:+88978612	MS.gene021696:CDS
AGGGGCGGAGGGGTAGTAAG+AGG	0.274253	7.4:-88978507	None:intergenic
TGCTCTCTCCGCCGCCTTAC+CGG	0.274469	7.4:+88978541	MS.gene021696:CDS
GGTGGTGGTGAGGAGGAATT+TGG	0.278567	7.4:-88978476	None:intergenic
GTGGAAAGTGGTGGTTCTTC+AGG	0.318129	7.4:-88978377	None:intergenic
ATCGTAGTGTTCTTAAGGTT+TGG	0.334173	7.4:+88978753	MS.gene021696:CDS
TTCATCAACATGGTTTGGTT+TGG	0.350778	7.4:-88978578	None:intergenic
TGTTGATCGTAGTGTTCTTA+AGG	0.355414	7.4:+88978748	MS.gene021696:CDS
CACAAGAATGTTGAAATTCA+TGG	0.370792	7.4:+88978881	MS.gene021696:CDS
CAGAATGAAGGTGGAGTTGT+TGG	0.373866	7.4:+88978923	MS.gene021696:CDS
GGAGTAACTGTTCGTGCTAA+TGG	0.375764	7.4:+88978944	MS.gene021696:CDS
AAGAAGCATGTGAGGGGCGG+AGG	0.375917	7.4:-88978519	None:intergenic
GAGAATTCATCAACATGGTT+TGG	0.380377	7.4:-88978583	None:intergenic
ATTTGGGCTCCGGTGGGAAA+AGG	0.392682	7.4:-88978459	None:intergenic
GTTCTTCAGGTTCACGGCGG+TGG	0.414474	7.4:-88978364	None:intergenic
CTGAAATTTGCTGAGAAAGT+TGG	0.415196	7.4:+88978668	MS.gene021696:CDS
CGGTGGGAAAAGGGTGGTGG+TGG	0.426898	7.4:-88978449	None:intergenic
GAAAAGGGTGGTGGTGGAAG+TGG	0.427112	7.4:-88978443	None:intergenic
CTCCGGTGGGAAAAGGGTGG+TGG	0.429757	7.4:-88978452	None:intergenic
GGAGGAGATCGGTGGTGGTG+AGG	0.437213	7.4:-88978486	None:intergenic
GGGTAGTAAGAGGAGGAGAT+CGG	0.438670	7.4:-88978497	None:intergenic
GGCGGAGGGGTAGTAAGAGG+AGG	0.440166	7.4:-88978504	None:intergenic
GTGGTGGTGAGGAGGAATTT+GGG	0.444504	7.4:-88978475	None:intergenic
GGGCTCCGGTGGGAAAAGGG+TGG	0.457386	7.4:-88978455	None:intergenic
GTTCACGGCGGTGGAAGTTA+CGG	0.458240	7.4:-88978355	None:intergenic
TTTAAGGATGTTGGGTCGGT+TGG	0.464594	7.4:-88978311	None:intergenic
TGAAATTTGCTGAGAAAGTT+GGG	0.467964	7.4:+88978669	MS.gene021696:CDS
GGGTCGGTTGGTGTTGCTGT+GGG	0.476226	7.4:-88978299	None:intergenic
GGAGATCGGTGGTGGTGAGG+AGG	0.490231	7.4:-88978483	None:intergenic
CACAATCATTATCAGAATGA+AGG	0.494541	7.4:+88978911	MS.gene021696:CDS
AGAAGCATGTGAGGGGCGGA+GGG	0.495592	7.4:-88978518	None:intergenic
GTGGTGGTTCTTCAGGTTCA+CGG	0.497308	7.4:-88978370	None:intergenic
TAAGAGGAGGAGATCGGTGG+TGG	0.498462	7.4:-88978491	None:intergenic
GTGAGGAGGAATTTGGGCTC+CGG	0.500275	7.4:-88978469	None:intergenic
GTGGTGGAAGTGGTTGATGA+CGG	0.500583	7.4:-88978433	None:intergenic
AGGAGGAATTTGGGCTCCGG+TGG	0.505710	7.4:-88978466	None:intergenic
TTTGGGCTCCGGTGGGAAAA+GGG	0.507171	7.4:-88978458	None:intergenic
AATAACAATGAGATTAATGA+TGG	0.520304	7.4:+88978821	MS.gene021696:CDS
ACATGAGTTGTGGTGGAAAG+TGG	0.548946	7.4:-88978389	None:intergenic
GAGAGAGCAAGAAGCATGTG+AGG	0.550052	7.4:-88978527	None:intergenic
GAAGCATGTGAGGGGCGGAG+GGG	0.553313	7.4:-88978517	None:intergenic
TGGGTCGGTTGGTGTTGCTG+TGG	0.558596	7.4:-88978300	None:intergenic
TCCTTAAAATGTGCTGCTTG+TGG	0.574232	7.4:+88978326	MS.gene021696:CDS
TAGTAAGAGGAGGAGATCGG+TGG	0.577001	7.4:-88978494	None:intergenic
TTGTGTTCTTCATCTTCAAG+AGG	0.585430	7.4:-88978863	None:intergenic
TTCAATGACATGAGTTGTGG+TGG	0.585624	7.4:-88978396	None:intergenic
AGCAAGAAGCATGTGAGGGG+CGG	0.594492	7.4:-88978522	None:intergenic
AGAGAGCAAGAAGCATGTGA+GGG	0.597359	7.4:-88978526	None:intergenic
AATCATTATCAGAATGAAGG+TGG	0.598316	7.4:+88978914	MS.gene021696:CDS
GTGGAAGTGGTTGATGACGG+TGG	0.599219	7.4:-88978430	None:intergenic
GAGAGCAAGAAGCATGTGAG+GGG	0.602424	7.4:-88978525	None:intergenic
ATATTCAATGACATGAGTTG+TGG	0.623812	7.4:-88978399	None:intergenic
AGTGGTTGATGACGGTGGTG+AGG	0.625162	7.4:-88978425	None:intergenic
GGAGGAATTTGGGCTCCGGT+GGG	0.630361	7.4:-88978465	None:intergenic
AGAGTGAGAATTCATCAACA+TGG	0.636406	7.4:-88978588	None:intergenic
TGAGTTGTGGTGGAAAGTGG+TGG	0.661282	7.4:-88978386	None:intergenic
CACGGCGGTGGAAGTTACGG+TGG	0.670619	7.4:-88978352	None:intergenic
AATTTGCTGAGAAAGTTGGG+TGG	0.726025	7.4:+88978672	MS.gene021696:CDS
GTGGTTCTTCAGGTTCACGG+CGG	0.747111	7.4:-88978367	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAACAATGAGATTAATGA+TGG	+	chr7.4:88978821-88978840	MS.gene021696:CDS	20.0%
	AATCATTATCAGAATGAAGG+TGG	+	chr7.4:88978914-88978933	MS.gene021696:CDS	30.0%
	ATATTCAATGACATGAGTTG+TGG	-	chr7.4:88978402-88978421	None:intergenic	30.0%
	CACAAGAATGTTGAAATTCA+TGG	+	chr7.4:88978881-88978900	MS.gene021696:CDS	30.0%
	CACAATCATTATCAGAATGA+AGG	+	chr7.4:88978911-88978930	MS.gene021696:CDS	30.0%
!!	TGAAATTTGCTGAGAAAGTT+GGG	+	chr7.4:88978669-88978688	MS.gene021696:CDS	30.0%
	AGAGTGAGAATTCATCAACA+TGG	-	chr7.4:88978591-88978610	None:intergenic	35.0%
	ATCGTAGTGTTCTTAAGGTT+TGG	+	chr7.4:88978753-88978772	MS.gene021696:CDS	35.0%
	GAGAATTCATCAACATGGTT+TGG	-	chr7.4:88978586-88978605	None:intergenic	35.0%
	TGTTGATCGTAGTGTTCTTA+AGG	+	chr7.4:88978748-88978767	MS.gene021696:CDS	35.0%
	TTGTGTTCTTCATCTTCAAG+AGG	-	chr7.4:88978866-88978885	None:intergenic	35.0%
!	CATGAGTTTTGCAATGAGAT+TGG	+	chr7.4:88978725-88978744	MS.gene021696:CDS	35.0%
!!	ACAATTCTAGAAAGCGTTTC+AGG	+	chr7.4:88978612-88978631	MS.gene021696:CDS	35.0%
!!	ACATTTTAAGGATGTTGGGT+CGG	-	chr7.4:88978318-88978337	None:intergenic	35.0%
!!	CAGCACATTTTAAGGATGTT+GGG	-	chr7.4:88978322-88978341	None:intergenic	35.0%
!!	CTGAAATTTGCTGAGAAAGT+TGG	+	chr7.4:88978668-88978687	MS.gene021696:CDS	35.0%
!!	TTCATCAACATGGTTTGGTT+TGG	-	chr7.4:88978581-88978600	None:intergenic	35.0%
	TTCAATGACATGAGTTGTGG+TGG	-	chr7.4:88978399-88978418	None:intergenic	40.0%
!!	AATTTGCTGAGAAAGTTGGG+TGG	+	chr7.4:88978672-88978691	MS.gene021696:CDS	40.0%
!!	GCAGCACATTTTAAGGATGT+TGG	-	chr7.4:88978323-88978342	None:intergenic	40.0%
!!	TCCTTAAAATGTGCTGCTTG+TGG	+	chr7.4:88978326-88978345	MS.gene021696:CDS	40.0%
	CAGAATGAAGGTGGAGTTGT+TGG	+	chr7.4:88978923-88978942	MS.gene021696:CDS	45.0%
	GGAGTAACTGTTCGTGCTAA+TGG	+	chr7.4:88978944-88978963	MS.gene021696:CDS	45.0%
	TTTAAGGATGTTGGGTCGGT+TGG	-	chr7.4:88978314-88978333	None:intergenic	45.0%
!	ACATGAGTTGTGGTGGAAAG+TGG	-	chr7.4:88978392-88978411	None:intergenic	45.0%
!	AGAGAGCAAGAAGCATGTGA+GGG	-	chr7.4:88978529-88978548	None:intergenic	45.0%
!	GCCACAAGCAGCACATTTTA+AGG	-	chr7.4:88978330-88978349	None:intergenic	45.0%
!!	TTTGGAGCTGCAACGTTTTC+CGG	-	chr7.4:88978563-88978582	None:intergenic	45.0%
	GGGTAGTAAGAGGAGGAGAT+CGG	-	chr7.4:88978500-88978519	None:intergenic	50.0%
	GTGGAAAGTGGTGGTTCTTC+AGG	-	chr7.4:88978380-88978399	None:intergenic	50.0%
	TAGTAAGAGGAGGAGATCGG+TGG	-	chr7.4:88978497-88978516	None:intergenic	50.0%
!	AGCTGCAACGTTTTCCGGTA+AGG	-	chr7.4:88978558-88978577	None:intergenic	50.0%
!	GAGAGAGCAAGAAGCATGTG+AGG	-	chr7.4:88978530-88978549	None:intergenic	50.0%
!	GAGAGCAAGAAGCATGTGAG+GGG	-	chr7.4:88978528-88978547	None:intergenic	50.0%
!	GTGGTGGTGAGGAGGAATTT+GGG	-	chr7.4:88978478-88978497	None:intergenic	50.0%
!	GTGGTGGTTCTTCAGGTTCA+CGG	-	chr7.4:88978373-88978392	None:intergenic	50.0%
!	TGAGTTGTGGTGGAAAGTGG+TGG	-	chr7.4:88978389-88978408	None:intergenic	50.0%
!	TGCAACGTTTTCCGGTAAGG+CGG	-	chr7.4:88978555-88978574	None:intergenic	50.0%
!!	GTGGTGGAAGTGGTTGATGA+CGG	-	chr7.4:88978436-88978455	None:intergenic	50.0%
	ATTTGGGCTCCGGTGGGAAA+AGG	-	chr7.4:88978462-88978481	None:intergenic	55.0%
	GTGAGGAGGAATTTGGGCTC+CGG	-	chr7.4:88978472-88978491	None:intergenic	55.0%
	GTTCACGGCGGTGGAAGTTA+CGG	-	chr7.4:88978358-88978377	None:intergenic	55.0%
	TAAGAGGAGGAGATCGGTGG+TGG	-	chr7.4:88978494-88978513	None:intergenic	55.0%
	TTTGGGCTCCGGTGGGAAAA+GGG	-	chr7.4:88978461-88978480	None:intergenic	55.0%
!	AACGTTTTCCGGTAAGGCGG+CGG	-	chr7.4:88978552-88978571	None:intergenic	55.0%
!	AGCAAGAAGCATGTGAGGGG+CGG	-	chr7.4:88978525-88978544	None:intergenic	55.0%
!	GGTGGTGGTGAGGAGGAATT+TGG	-	chr7.4:88978479-88978498	None:intergenic	55.0%
!	GTGGTTCTTCAGGTTCACGG+CGG	-	chr7.4:88978370-88978389	None:intergenic	55.0%
!!	AGTGGTTGATGACGGTGGTG+AGG	-	chr7.4:88978428-88978447	None:intergenic	55.0%
!!	GAAAAGGGTGGTGGTGGAAG+TGG	-	chr7.4:88978446-88978465	None:intergenic	55.0%
!!	GTGGAAGTGGTTGATGACGG+TGG	-	chr7.4:88978433-88978452	None:intergenic	55.0%
	AGGAGGAATTTGGGCTCCGG+TGG	-	chr7.4:88978469-88978488	None:intergenic	60.0%
	GGAGGAATTTGGGCTCCGGT+GGG	-	chr7.4:88978468-88978487	None:intergenic	60.0%
!	AAGAAGCATGTGAGGGGCGG+AGG	-	chr7.4:88978522-88978541	None:intergenic	60.0%
!	AGAAGCATGTGAGGGGCGGA+GGG	-	chr7.4:88978521-88978540	None:intergenic	60.0%
!	CACCACCACCCTTTTCCCAC+CGG	+	chr7.4:88978450-88978469	MS.gene021696:CDS	60.0%
!	GTTCTTCAGGTTCACGGCGG+TGG	-	chr7.4:88978367-88978386	None:intergenic	60.0%
!!	GGGTCGGTTGGTGTTGCTGT+GGG	-	chr7.4:88978302-88978321	None:intergenic	60.0%
!!	TGGGTCGGTTGGTGTTGCTG+TGG	-	chr7.4:88978303-88978322	None:intergenic	60.0%
	AGGGGCGGAGGGGTAGTAAG+AGG	-	chr7.4:88978510-88978529	None:intergenic	65.0%
	CACGGCGGTGGAAGTTACGG+TGG	-	chr7.4:88978355-88978374	None:intergenic	65.0%
	CTCCGGTGGGAAAAGGGTGG+TGG	-	chr7.4:88978455-88978474	None:intergenic	65.0%
	GAAGCATGTGAGGGGCGGAG+GGG	-	chr7.4:88978520-88978539	None:intergenic	65.0%
	GGCGGAGGGGTAGTAAGAGG+AGG	-	chr7.4:88978507-88978526	None:intergenic	65.0%
	TGCTCTCTCCGCCGCCTTAC+CGG	+	chr7.4:88978541-88978560	MS.gene021696:CDS	65.0%
!	CGGTGGGAAAAGGGTGGTGG+TGG	-	chr7.4:88978452-88978471	None:intergenic	65.0%
!	GGAGATCGGTGGTGGTGAGG+AGG	-	chr7.4:88978486-88978505	None:intergenic	65.0%
!	GGAGGAGATCGGTGGTGGTG+AGG	-	chr7.4:88978489-88978508	None:intergenic	65.0%
	GGGCTCCGGTGGGAAAAGGG+TGG	-	chr7.4:88978458-88978477	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	88978290	88978985	88978290	ID=MS.gene021696
chr7.4	mRNA	88978290	88978985	88978290	ID=MS.gene021696.t1;Parent=MS.gene021696
chr7.4	exon	88978290	88978985	88978290	ID=MS.gene021696.t1.exon1;Parent=MS.gene021696.t1
chr7.4	CDS	88978290	88978985	88978290	ID=cds.MS.gene021696.t1;Parent=MS.gene021696.t1

Gene Sequence

>MS.gene021696

ATGACATCACCCACAGCAACACCAACCGACCCAACATCCTTAAAATGTGCTGCTTGTGGCTGCCACCGTAACTTCCACCGCCGTGAACCTGAAGAACCACCACTTTCCACCACAACTCATGTCATTGAATATCAACCTCACCACCGTCATCAACCACTTCCACCACCACCCTTTTCCCACCGGAGCCCAAATTCCTCCTCACCACCACCGATCTCCTCCTCTTACTACCCCTCCGCCCCTCACATGCTTCTTGCTCTCTCCGCCGCCTTACCGGAAAACGTTGCAGCTCCAAACCAAACCATGTTGATGAATTCTCACTCTAACAATTCTAGAAAGCGTTTCAGGACAAAGTTTACTCAAGATCAGAAAGATAAGATGCTGAAATTTGCTGAGAAAGTTGGGTGGAAGATGCAGAAAAAAGATGATGAATTTGTTCATGAGTTTTGCAATGAGATTGGTGTTGATCGTAGTGTTCTTAAGGTTTGGATGCATAACAACAAGAACACTTTAGCTAAACGAGAAAATCTTAACAATAACAATGAGATTAATGATGGTGTTAAGAGTTTTCAGCTACCTCTTGAAGATGAAGAACACAAGAATGTTGAAATTCATGGTCTTAATCACAATCATTATCAGAATGAAGGTGGAGTTGTTGGAGTAACTGTTCGTGCTAATGGTTCATCATCTTCTTCTTAA

Protein sequence

>MS.gene021696.t1

MTSPTATPTDPTSLKCAACGCHRNFHRREPEEPPLSTTTHVIEYQPHHRHQPLPPPPFSHRSPNSSSPPPISSSYYPSAPHMLLALSAALPENVAAPNQTMLMNSHSNNSRKRFRTKFTQDQKDKMLKFAEKVGWKMQKKDDEFVHEFCNEIGVDRSVLKVWMHNNKNTLAKRENLNNNNEINDGVKSFQLPLEDEEHKNVEIHGLNHNHYQNEGGVVGVTVRANGSSSSS