AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43476


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43476.t1 MTR_4g134580 93.950 281 11 2 1 275 85 365 0.0 546
MS.gene43476.t1 MTR_1g077020 44.017 234 81 4 25 258 5 188 1.19e-62 196
MS.gene43476.t1 MTR_5g020510 42.194 237 106 5 22 258 92 297 2.27e-55 181
MS.gene43476.t1 MTR_3g100650 41.772 237 104 5 20 256 71 273 6.04e-53 174
MS.gene43476.t1 MTR_5g065440 42.149 242 98 7 27 256 56 267 8.67e-51 168
MS.gene43476.t1 MTR_7g010300 38.889 234 106 7 26 257 85 283 3.44e-44 153
MS.gene43476.t1 MTR_2g076780 38.494 239 113 8 21 256 29 236 1.46e-42 145
MS.gene43476.t1 MTR_8g069080 33.610 241 127 6 14 251 52 262 1.89e-34 125
MS.gene43476.t1 MTR_5g032600 73.016 63 17 0 194 256 209 271 1.17e-25 102
MS.gene43476.t1 MTR_6g012280 52.874 87 40 1 183 269 211 296 3.77e-25 102
MS.gene43476.t1 MTR_8g467340 58.824 68 28 0 194 261 271 338 1.24e-22 96.3
MS.gene43476.t1 MTR_8g467340 63.934 61 20 1 22 80 57 117 2.19e-18 84.3
MS.gene43476.t1 MTR_3g039810 56.757 74 30 1 12 83 1 74 1.97e-20 83.6
MS.gene43476.t1 MTR_8g069380 58.730 63 26 0 194 256 52 114 1.49e-19 82.0
MS.gene43476.t1 MTR_6g073013 55.000 60 26 1 194 252 101 160 8.66e-19 82.0
MS.gene43476.t1 MTR_6g073013 60.000 50 19 1 29 77 19 68 8.76e-15 71.2
MS.gene43476.t1 MTR_8g051480 59.677 62 24 1 195 256 68 128 4.44e-18 78.6
MS.gene43476.t1 MTR_6g012220 61.538 52 16 1 26 77 18 65 2.53e-14 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43476.t1 AT2G02540 47.581 248 84 5 17 256 75 284 5.30e-69 216
MS.gene43476.t1 AT2G02540 47.581 248 84 5 17 256 75 284 5.30e-69 216
MS.gene43476.t1 AT1G14440 47.436 234 80 5 26 256 87 280 1.06e-62 200
MS.gene43476.t1 AT1G14440 47.436 234 80 5 26 256 87 280 1.06e-62 200
MS.gene43476.t1 AT2G18350 39.464 261 88 7 10 258 60 262 5.16e-49 163
MS.gene43476.t1 AT1G69600 38.075 239 96 7 27 259 29 221 2.34e-45 153
MS.gene43476.t1 AT5G60480 32.751 229 95 5 25 251 33 204 7.32e-36 128
MS.gene43476.t1 AT3G50890 31.004 229 114 4 27 255 58 242 1.27e-28 110
MS.gene43476.t1 AT1G75240 78.571 56 12 0 26 81 73 128 1.48e-26 105
MS.gene43476.t1 AT4G24660 64.516 62 22 0 195 256 158 219 5.36e-24 97.1
MS.gene43476.t1 AT4G24660 72.222 54 15 0 27 80 47 100 3.67e-23 94.7
MS.gene43476.t1 AT5G65410 60.317 63 25 0 193 255 190 252 3.45e-23 96.3
MS.gene43476.t1 AT5G65410 71.154 52 15 0 28 79 74 125 2.79e-21 90.9
MS.gene43476.t1 AT4G24660 72.222 54 15 0 27 80 47 100 3.96e-23 95.1
MS.gene43476.t1 AT4G24660 56.164 73 32 0 195 267 158 230 4.09e-23 95.1
MS.gene43476.t1 AT5G39760 51.220 82 38 1 194 273 200 281 8.95e-22 93.2
MS.gene43476.t1 AT1G74660 66.667 57 19 0 27 83 37 93 2.61e-21 86.3
MS.gene43476.t1 AT5G15210 63.158 57 21 0 194 250 179 235 2.85e-21 90.9
MS.gene43476.t1 AT5G15210 61.818 55 19 1 26 78 53 107 2.26e-16 77.4
MS.gene43476.t1 AT1G74660 66.667 57 19 0 27 83 37 93 3.22e-21 86.3
MS.gene43476.t1 AT3G28920 60.000 60 24 0 194 253 192 251 1.63e-20 89.4
MS.gene43476.t1 AT3G28917 65.517 58 19 1 27 83 31 88 3.47e-20 83.6
MS.gene43476.t1 AT1G18835 63.158 57 18 1 27 83 24 77 5.91e-17 74.3
MS.gene43476.t1 AT5G42780 59.259 54 20 1 29 82 64 115 2.28e-13 68.6
MS.gene43476.t1 AT5G42780 45.763 59 32 0 195 253 180 238 7.85e-13 67.0

Find 62 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGATCAGGCTCTTGGTCT+TGG 0.311106 8.4:-277025 MS.gene43476:CDS
CTCTTCTTGTTCATCAGATT+CGG 0.338219 8.4:+276862 None:intergenic
AATGGTTCCTTGTTCACCAC+TGG 0.354032 8.4:+277210 None:intergenic
GTTGCTGCTGATGCTGTATT+AGG 0.369444 8.4:+276980 None:intergenic
CCTCTTCCTGATCAGGCTCT+TGG 0.375629 8.4:-277031 MS.gene43476:CDS
CATGTTACCTCTTCCTGATC+AGG 0.387160 8.4:-277038 MS.gene43476:CDS
CCTTCAACTTCAACTTCAAC+AGG 0.390687 8.4:-277087 MS.gene43476:CDS
CCTGTTGAAGTTGAAGTTGA+AGG 0.398274 8.4:+277087 None:intergenic
AGAGCAGTTGAGTGCTTCAA+TGG 0.440838 8.4:+277192 None:intergenic
AAAGAAGAGTGCTCAAGGTT+TGG 0.446033 8.4:-276655 MS.gene43476:CDS
GAAGGTATGTTGTAGTTGAA+TGG 0.452483 8.4:+276884 None:intergenic
CCTGATCCTCAGTCTCACCT+TGG 0.455452 8.4:+277131 None:intergenic
CCAAGACCAAGAGCCTGATC+AGG 0.460517 8.4:+277025 None:intergenic
CCAAGGTGAGACTGAGGATC+AGG 0.460530 8.4:-277131 MS.gene43476:CDS
AACTGATGACGATTTCTTGT+AGG 0.461489 8.4:+277315 None:intergenic
AGCAGGTAGTGATGAAGAAA+AGG 0.463951 8.4:-276802 MS.gene43476:CDS
TAGTGATGAAGAAAAGGTTC+AGG 0.467781 8.4:-276796 MS.gene43476:CDS
CAACAGTTCTGCCAAGAGAT+TGG 0.479137 8.4:-276683 MS.gene43476:CDS
TAGGAGTAGAGTGGTGGTGT+TGG 0.483759 8.4:+276999 None:intergenic
AATACAGCATCAGCAGCAAC+AGG 0.487107 8.4:-276977 MS.gene43476:CDS
AAGAATCATGCAGCTGCAAT+GGG 0.493612 8.4:-277268 MS.gene43476:CDS
ATGGTTCCTTGTTCACCACT+GGG 0.498200 8.4:+277211 None:intergenic
AAAGAATCATGCAGCTGCAA+TGG 0.500219 8.4:-277269 MS.gene43476:CDS
GGCAGCACCACCACAGCATG+AGG 0.512800 8.4:-277066 MS.gene43476:CDS
CAAGCAGCAGCTGTTAGTAC+TGG 0.515000 8.4:-277364 MS.gene43476:CDS
AGAGCTGTAGCGCCACCTCA+TGG 0.521678 8.4:-276926 MS.gene43476:CDS
GGTGGCGCTACAGCTCTAGA+AGG 0.538474 8.4:+276932 None:intergenic
CGAATCTGATGAACAAGAAG+AGG 0.550515 8.4:-276861 MS.gene43476:CDS
GAAGTTTCTGTGACAATGGC+AGG 0.555632 8.4:+277168 None:intergenic
CCAAGAGCCTGATCAGGAAG+AGG 0.556475 8.4:+277031 None:intergenic
TCTTGTTCATCAGATTCGGA+AGG 0.560538 8.4:+276866 None:intergenic
CTAACAGCTGCTGCTTGCAT+AGG 0.566961 8.4:+277370 None:intergenic
AAAATTCACACAGGAACAGA+AGG 0.568057 8.4:-276771 MS.gene43476:CDS
TTCATGCCCAGTGGTGAACA+AGG 0.570431 8.4:-277217 MS.gene43476:CDS
TGTGGAAGTTTCTGTGACAA+TGG 0.570726 8.4:+277164 None:intergenic
CAAGAAATCGTCATCAGTTG+TGG 0.580092 8.4:-277311 MS.gene43476:CDS
GATGAACAAGAAGAGGCAGC+AGG 0.580135 8.4:-276854 MS.gene43476:CDS
TGATGACGATTTCTTGTAGG+AGG 0.584121 8.4:+277318 None:intergenic
AATCATGCAGCTGCAATGGG+AGG 0.592565 8.4:-277265 MS.gene43476:CDS
GTAGCGCCACCTCATGGACA+TGG 0.593213 8.4:-276920 MS.gene43476:CDS
TGAATTTCCTCATGCTGTGG+TGG 0.602127 8.4:+277059 None:intergenic
TGTGAAAAGAAGAGTGCTCA+AGG 0.602235 8.4:-276660 MS.gene43476:CDS
ATCATGCAGCTGCAATGGGA+GGG 0.602761 8.4:-277264 MS.gene43476:CDS
ATGGAAATTTCAGAAGCAAG+AGG 0.613533 8.4:-276717 MS.gene43476:CDS
GGGAATGCCACAGATGGCTG+TGG 0.614228 8.4:-277244 MS.gene43476:CDS
TGAAGTTGAAGGCTGAGTGA+TGG 0.622721 8.4:+277098 None:intergenic
AGTTGAAGGCTGAGTGATGG+TGG 0.623553 8.4:+277101 None:intergenic
ATGGGAGGGAATGCCACAGA+TGG 0.625053 8.4:-277250 MS.gene43476:CDS
AGAAGTCCAAGGTGAGACTG+AGG 0.639644 8.4:-277137 MS.gene43476:CDS
AGCAGCTGTTAGTACTGGTG+TGG 0.641970 8.4:-277359 MS.gene43476:CDS
TTCCACAGAAAAGAAGTCCA+AGG 0.650236 8.4:-277148 MS.gene43476:CDS
TCAGAAGCAAGAGGAATCTG+TGG 0.650938 8.4:-276708 MS.gene43476:CDS
AGTTGAATGGCATGATGATG+TGG 0.657862 8.4:+276897 None:intergenic
ATGTGGCCATGTCCATGAGG+TGG 0.659218 8.4:+276914 None:intergenic
ACATGAATTTCCTCATGCTG+TGG 0.665457 8.4:+277056 None:intergenic
AATCGTCATCAGTTGTGGTG+AGG 0.674142 8.4:-277306 MS.gene43476:CDS
CTGTATTAGGAGTAGAGTGG+TGG 0.678813 8.4:+276993 None:intergenic
TGTGGTGAGTTCATGCCCAG+TGG 0.682320 8.4:-277226 MS.gene43476:CDS
GAACTCACCACAGCCATCTG+TGG 0.688684 8.4:+277237 None:intergenic
GTTCAGGACAAAATTCACAC+AGG 0.704613 8.4:-276780 MS.gene43476:CDS
ATGCTGTATTAGGAGTAGAG+TGG 0.716681 8.4:+276990 None:intergenic
ATGATGTGGCCATGTCCATG+AGG 0.730801 8.4:+276911 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCATGTTGAAGTTGTTTTT+TGG + chr8.4:277325-277344 None:intergenic 25.0%
! TTCAATTTTGCTGAGAAAGT+TGG - chr8.4:277189-277208 MS.gene43476:CDS 30.0%
AAAATTCACACAGGAACAGA+AGG - chr8.4:277158-277177 MS.gene43476:CDS 35.0%
ATGGAAATTTCAGAAGCAAG+AGG - chr8.4:277212-277231 MS.gene43476:CDS 35.0%
CTCTTCTTGTTCATCAGATT+CGG + chr8.4:277070-277089 None:intergenic 35.0%
TAGTGATGAAGAAAAGGTTC+AGG - chr8.4:277133-277152 MS.gene43476:CDS 35.0%
! AACTGATGACGATTTCTTGT+AGG + chr8.4:276617-276636 None:intergenic 35.0%
! ATTTTGCTGAGAAAGTTGGA+TGG - chr8.4:277193-277212 MS.gene43476:CDS 35.0%
! GAAGGTATGTTGTAGTTGAA+TGG + chr8.4:277048-277067 None:intergenic 35.0%
! TTCTTTTCACACCAATCTCT+TGG + chr8.4:277260-277279 None:intergenic 35.0%
AAAGAATCATGCAGCTGCAA+TGG - chr8.4:276660-276679 MS.gene43476:CDS 40.0%
AAGAATCATGCAGCTGCAAT+GGG - chr8.4:276661-276680 MS.gene43476:CDS 40.0%
ACATGAATTTCCTCATGCTG+TGG + chr8.4:276876-276895 None:intergenic 40.0%
AGCAGGTAGTGATGAAGAAA+AGG - chr8.4:277127-277146 MS.gene43476:CDS 40.0%
ATGCTGTATTAGGAGTAGAG+TGG + chr8.4:276942-276961 None:intergenic 40.0%
CAAGAAATCGTCATCAGTTG+TGG - chr8.4:276618-276637 MS.gene43476:CDS 40.0%
CCTGTTGAAGTTGAAGTTGA+AGG + chr8.4:276845-276864 None:intergenic 40.0%
CCTTCAACTTCAACTTCAAC+AGG - chr8.4:276842-276861 MS.gene43476:CDS 40.0%
CGAATCTGATGAACAAGAAG+AGG - chr8.4:277068-277087 MS.gene43476:CDS 40.0%
GTTCAGGACAAAATTCACAC+AGG - chr8.4:277149-277168 MS.gene43476:CDS 40.0%
TCTTGTTCATCAGATTCGGA+AGG + chr8.4:277066-277085 None:intergenic 40.0%
TGTGGAAGTTTCTGTGACAA+TGG + chr8.4:276768-276787 None:intergenic 40.0%
TTCCACAGAAAAGAAGTCCA+AGG - chr8.4:276781-276800 MS.gene43476:CDS 40.0%
! AGTTGAATGGCATGATGATG+TGG + chr8.4:277035-277054 None:intergenic 40.0%
! TGATGACGATTTCTTGTAGG+AGG + chr8.4:276614-276633 None:intergenic 40.0%
!! AAAGAAGAGTGCTCAAGGTT+TGG - chr8.4:277274-277293 MS.gene43476:CDS 40.0%
!! TGTGAAAAGAAGAGTGCTCA+AGG - chr8.4:277269-277288 MS.gene43476:CDS 40.0%
AATACAGCATCAGCAGCAAC+AGG - chr8.4:276952-276971 MS.gene43476:CDS 45.0%
AATCGTCATCAGTTGTGGTG+AGG - chr8.4:276623-276642 MS.gene43476:CDS 45.0%
AATGGTTCCTTGTTCACCAC+TGG + chr8.4:276722-276741 None:intergenic 45.0%
AGAGCAGTTGAGTGCTTCAA+TGG + chr8.4:276740-276759 None:intergenic 45.0%
ATGGTTCCTTGTTCACCACT+GGG + chr8.4:276721-276740 None:intergenic 45.0%
CAACAGTTCTGCCAAGAGAT+TGG - chr8.4:277246-277265 MS.gene43476:CDS 45.0%
CATGTTACCTCTTCCTGATC+AGG - chr8.4:276891-276910 MS.gene43476:CDS 45.0%
CTGTATTAGGAGTAGAGTGG+TGG + chr8.4:276939-276958 None:intergenic 45.0%
GAAGTTTCTGTGACAATGGC+AGG + chr8.4:276764-276783 None:intergenic 45.0%
GTTGCTGCTGATGCTGTATT+AGG + chr8.4:276952-276971 None:intergenic 45.0%
TCAGAAGCAAGAGGAATCTG+TGG - chr8.4:277221-277240 MS.gene43476:CDS 45.0%
TCATCATCATCATCAGCAGC+AGG - chr8.4:277110-277129 MS.gene43476:CDS 45.0%
TGAAGTTGAAGGCTGAGTGA+TGG + chr8.4:276834-276853 None:intergenic 45.0%
TGAATTTCCTCATGCTGTGG+TGG + chr8.4:276873-276892 None:intergenic 45.0%
! CACCTTGGACTTCTTTTCTG+TGG + chr8.4:276786-276805 None:intergenic 45.0%
AATCATGCAGCTGCAATGGG+AGG - chr8.4:276664-276683 MS.gene43476:CDS 50.0%
AGAAGTCCAAGGTGAGACTG+AGG - chr8.4:276792-276811 MS.gene43476:CDS 50.0%
AGTTGAAGGCTGAGTGATGG+TGG + chr8.4:276831-276850 None:intergenic 50.0%
ATCATGCAGCTGCAATGGGA+GGG - chr8.4:276665-276684 MS.gene43476:CDS 50.0%
ATGATGTGGCCATGTCCATG+AGG + chr8.4:277021-277040 None:intergenic 50.0%
CAAGCAGCAGCTGTTAGTAC+TGG - chr8.4:276565-276584 MS.gene43476:CDS 50.0%
GATGAACAAGAAGAGGCAGC+AGG - chr8.4:277075-277094 MS.gene43476:CDS 50.0%
TTCATGCCCAGTGGTGAACA+AGG - chr8.4:276712-276731 MS.gene43476:CDS 50.0%
! TAGGAGTAGAGTGGTGGTGT+TGG + chr8.4:276933-276952 None:intergenic 50.0%
!! AGCAGCTGTTAGTACTGGTG+TGG - chr8.4:276570-276589 MS.gene43476:CDS 50.0%
ATGGGAGGGAATGCCACAGA+TGG - chr8.4:276679-276698 MS.gene43476:CDS 55.0%
ATGTGGCCATGTCCATGAGG+TGG + chr8.4:277018-277037 None:intergenic 55.0%
CCAAGACCAAGAGCCTGATC+AGG + chr8.4:276907-276926 None:intergenic 55.0%
CCAAGAGCCTGATCAGGAAG+AGG + chr8.4:276901-276920 None:intergenic 55.0%
CCAAGGTGAGACTGAGGATC+AGG - chr8.4:276798-276817 MS.gene43476:CDS 55.0%
CCTCTTCCTGATCAGGCTCT+TGG - chr8.4:276898-276917 MS.gene43476:CDS 55.0%
CCTGATCAGGCTCTTGGTCT+TGG - chr8.4:276904-276923 MS.gene43476:CDS 55.0%
CCTGATCCTCAGTCTCACCT+TGG + chr8.4:276801-276820 None:intergenic 55.0%
GAACTCACCACAGCCATCTG+TGG + chr8.4:276695-276714 None:intergenic 55.0%
TGTGGTGAGTTCATGCCCAG+TGG - chr8.4:276703-276722 MS.gene43476:CDS 55.0%
AGAGCTGTAGCGCCACCTCA+TGG - chr8.4:277003-277022 MS.gene43476:CDS 60.0%
GGGAATGCCACAGATGGCTG+TGG - chr8.4:276685-276704 MS.gene43476:CDS 60.0%
GTAGCGCCACCTCATGGACA+TGG - chr8.4:277009-277028 MS.gene43476:CDS 60.0%
!! GGTGGCGCTACAGCTCTAGA+AGG + chr8.4:277000-277019 None:intergenic 60.0%
GGCAGCACCACCACAGCATG+AGG - chr8.4:276863-276882 MS.gene43476:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 276562 277389 276562 ID=MS.gene43476
chr8.4 mRNA 276562 277389 276562 ID=MS.gene43476.t1;Parent=MS.gene43476
chr8.4 exon 276562 277389 276562 ID=MS.gene43476.t1.exon1;Parent=MS.gene43476.t1
chr8.4 CDS 276562 277389 276562 ID=cds.MS.gene43476.t1;Parent=MS.gene43476.t1
Gene Sequence

>MS.gene43476

ATGCAAGCAGCAGCTGTTAGTACTGGTGTGGAATTAGATCATGATCAGTCCTCCTACAAGAAATCGTCATCAGTTGTGGTGAGGTACAGAGAATGTCTAAAGAATCATGCAGCTGCAATGGGAGGGAATGCCACAGATGGCTGTGGTGAGTTCATGCCCAGTGGTGAACAAGGAACCATTGAAGCACTCAACTGCTCTGCCTGCCATTGTCACAGAAACTTCCACAGAAAAGAAGTCCAAGGTGAGACTGAGGATCAGGAAAACTACCACCATCACTCAGCCTTCAACTTCAACTTCAACAGGCAGCACCACCACAGCATGAGGAAATTCATGTTACCTCTTCCTGATCAGGCTCTTGGTCTTGGCTACCAACACCACCACTCTACTCCTAATACAGCATCAGCAGCAACAGGTAACAACAACAACAACATTCTTCCTTCTAGAGCTGTAGCGCCACCTCATGGACATGGCCACATCATCATGCCATTCAACTACAACATACCTTCCGAATCTGATGAACAAGAAGAGGCAGCAGGTAGACTACATATTCATCATCATCATCAGCAGCAGGTAGTGATGAAGAAAAGGTTCAGGACAAAATTCACACAGGAACAGAAGGACAAAATGTTCAATTTTGCTGAGAAAGTTGGATGGAAATTTCAGAAGCAAGAGGAATCTGTGGTGCAACAGTTCTGCCAAGAGATTGGTGTGAAAAGAAGAGTGCTCAAGGTTTGGATGCATAACAATAAACACAATCTCGCCAAAAAACAACTTCAACATGAACATATTACTGCTAGTGCTAGTGCTAATCATCTCCCAACTCCATAA

Protein sequence

>MS.gene43476.t1

MQAAAVSTGVELDHDQSSYKKSSSVVVRYRECLKNHAAAMGGNATDGCGEFMPSGEQGTIEALNCSACHCHRNFHRKEVQGETEDQENYHHHSAFNFNFNRQHHHSMRKFMLPLPDQALGLGYQHHHSTPNTASAATGNNNNNILPSRAVAPPHGHGHIIMPFNYNIPSESDEQEEAAGRLHIHHHHQQQVVMKKRFRTKFTQEQKDKMFNFAEKVGWKFQKQEESVVQQFCQEIGVKRRVLKVWMHNNKHNLAKKQLQHEHITASASANHLPTP