Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60343.t1 | XP_003608955.1 | 93.1 | 188 | 12 | 1 | 1 | 188 | 1 | 187 | 2.10E-72 | 282 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60343.t1 | G7JNU9 | 93.1 | 188 | 12 | 1 | 1 | 188 | 1 | 187 | 1.5e-72 | 282.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051184 | MS.gene60343 | 0.800602 | 1.33E-48 | -1.69E-46 |
| MS.gene051757 | MS.gene60343 | 0.822748 | 2.01E-53 | -1.69E-46 |
| MS.gene051790 | MS.gene60343 | 0.803144 | 4.01E-49 | -1.69E-46 |
| MS.gene051831 | MS.gene60343 | 0.807969 | 3.90E-50 | -1.69E-46 |
| MS.gene055864 | MS.gene60343 | 0.817361 | 3.43E-52 | -1.69E-46 |
| MS.gene057194 | MS.gene60343 | 0.801573 | 8.45E-49 | -1.69E-46 |
| MS.gene058543 | MS.gene60343 | 0.80094 | 1.14E-48 | -1.69E-46 |
| MS.gene059283 | MS.gene60343 | 0.807228 | 5.60E-50 | -1.69E-46 |
| MS.gene059774 | MS.gene60343 | 0.806702 | 7.23E-50 | -1.69E-46 |
| MS.gene060697 | MS.gene60343 | 0.805495 | 1.30E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene60343 | MS.gene074378 | PPI |
| MS.gene60343 | MS.gene022274 | PPI |
| MS.gene60343 | MS.gene25931 | PPI |
| MS.gene70603 | MS.gene60343 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60343.t1 | MTR_4g106790 | 97.340 | 188 | 4 | 1 | 1 | 188 | 1 | 187 | 3.40e-134 | 373 |
| MS.gene60343.t1 | MTR_4g106790 | 95.973 | 149 | 5 | 1 | 1 | 149 | 1 | 148 | 1.02e-96 | 278 |
| MS.gene60343.t1 | MTR_3g078300 | 54.974 | 191 | 75 | 3 | 1 | 188 | 1 | 183 | 3.05e-63 | 193 |
| MS.gene60343.t1 | MTR_3g078300 | 55.263 | 152 | 57 | 3 | 1 | 149 | 1 | 144 | 5.17e-48 | 155 |
| MS.gene60343.t1 | MTR_5g035430 | 42.328 | 189 | 87 | 4 | 1 | 186 | 1 | 170 | 8.86e-40 | 133 |
| MS.gene60343.t1 | MTR_4g097220 | 38.624 | 189 | 94 | 4 | 1 | 188 | 1 | 168 | 8.16e-35 | 120 |
| MS.gene60343.t1 | MTR_8g063200 | 38.378 | 185 | 99 | 4 | 1 | 185 | 1 | 170 | 5.27e-30 | 108 |
| MS.gene60343.t1 | MTR_4g097220 | 42.537 | 134 | 57 | 2 | 1 | 134 | 1 | 114 | 2.92e-29 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60343.t1 | AT5G62900 | 46.809 | 188 | 73 | 4 | 1 | 188 | 1 | 161 | 8.99e-48 | 153 |
| MS.gene60343.t1 | AT5G50090 | 44.444 | 189 | 73 | 4 | 1 | 188 | 1 | 158 | 6.41e-42 | 139 |
| MS.gene60343.t1 | AT5G50090 | 46.277 | 188 | 65 | 5 | 1 | 188 | 1 | 152 | 2.68e-41 | 137 |
| MS.gene60343.t1 | AT1G60010 | 41.489 | 188 | 95 | 4 | 1 | 188 | 1 | 173 | 1.22e-40 | 135 |
| MS.gene60343.t1 | AT5G50090 | 52.500 | 120 | 43 | 1 | 1 | 120 | 1 | 106 | 1.09e-35 | 122 |
| MS.gene60343.t1 | AT1G10530 | 35.638 | 188 | 99 | 3 | 1 | 188 | 1 | 166 | 8.43e-35 | 120 |
| MS.gene60343.t1 | AT5G67620 | 32.195 | 205 | 98 | 7 | 1 | 188 | 1 | 181 | 1.03e-23 | 92.8 |
Find 25 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCTTTCTTCTTTGCCATT+TGG | 0.302263 | 8.2:+19112142 | None:intergenic |
| ACAAAAGTCAAATCAGATTA+AGG | 0.303462 | 8.2:-19111693 | MS.gene60343:CDS |
| AGATTTACCTTGTTATCTTC+AGG | 0.363890 | 8.2:+19111635 | None:intergenic |
| TTAATGATAAAACAGCTAAT+AGG | 0.376595 | 8.2:-19111664 | MS.gene60343:CDS |
| ATCACTTCACTAGCAGTAAC+TGG | 0.406024 | 8.2:+19112112 | None:intergenic |
| AGAAGAGCAACATAGTGATC+AGG | 0.427746 | 8.2:+19112079 | None:intergenic |
| CAGAGGTTACAAAGGGTATG+TGG | 0.456771 | 8.2:-19111763 | MS.gene60343:CDS |
| TTCAGTGATGCTTTGTAAAG+AGG | 0.477664 | 8.2:+19111076 | None:intergenic |
| GCAAAGAAGAAAGGTTGTAT+TGG | 0.478560 | 8.2:-19112135 | MS.gene60343:CDS |
| AGGACAAAAGCAACAACAAA+TGG | 0.492369 | 8.2:-19111134 | MS.gene60343:CDS |
| TCAGTGATGCTTTGTAAAGA+GGG | 0.514695 | 8.2:+19111077 | None:intergenic |
| AACCAAATGGCAAAGAAGAA+AGG | 0.522282 | 8.2:-19112144 | MS.gene60343:CDS |
| TTGGTTTCAGAGGTTACAAA+GGG | 0.536135 | 8.2:-19111770 | MS.gene60343:intron |
| GATTCAAAACTCATATCAGC+TGG | 0.538326 | 8.2:+19111049 | None:intergenic |
| CTTGTGATACAGCAACCAAA+TGG | 0.541988 | 8.2:-19112157 | MS.gene60343:CDS |
| GAAGAGCAACATAGTGATCA+GGG | 0.575247 | 8.2:+19112080 | None:intergenic |
| CCAAGCAAAAGTGTATCTGT+TGG | 0.576593 | 8.2:+19111935 | None:intergenic |
| TTTGGTTTCAGAGGTTACAA+AGG | 0.584060 | 8.2:-19111771 | MS.gene60343:intron |
| AGAGGTTACAAAGGGTATGT+GGG | 0.585748 | 8.2:-19111762 | MS.gene60343:CDS |
| TATAGACTCATCTCAGCTCA+AGG | 0.593800 | 8.2:-19111905 | MS.gene60343:intron |
| GTCTGAACCTGAAGATAACA+AGG | 0.608807 | 8.2:-19111642 | MS.gene60343:intron |
| AGTCAAATCAGATTAAGGAA+AGG | 0.621653 | 8.2:-19111688 | MS.gene60343:CDS |
| AATCTGAAAGACATGAGTCA+AGG | 0.637514 | 8.2:-19111154 | MS.gene60343:CDS |
| ACAAAAGCAACAACAAATGG+TGG | 0.646145 | 8.2:-19111131 | MS.gene60343:CDS |
| ATAATAATAAGTCAAGAACA+TGG | 0.691937 | 8.2:-19111103 | MS.gene60343:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATTAATAATAATTTATGAT+TGG | - | chr8.2:19112056-19112075 | MS.gene60343:CDS | 10.0% |
| !! | GATAATGCATAATATATATA+AGG | + | chr8.2:19111851-19111870 | None:intergenic | 15.0% |
| !! | TATAAAGAATCAAAAGAAAA+AGG | + | chr8.2:19111405-19111424 | None:intergenic | 15.0% |
| !! | TTATGTACATTAACTAATTA+AGG | - | chr8.2:19111909-19111928 | MS.gene60343:CDS | 15.0% |
| !!! | AAGAACAAAATAATCTTTTA+AGG | + | chr8.2:19111439-19111458 | None:intergenic | 15.0% |
| !!! | AGAACAAAATAATCTTTTAA+GGG | + | chr8.2:19111438-19111457 | None:intergenic | 15.0% |
| !!! | TACACATTGATTATTTTTTT+TGG | - | chr8.2:19111812-19111831 | MS.gene60343:intron | 15.0% |
| !! | ATAATAATAAGTCAAGAACA+TGG | - | chr8.2:19112151-19112170 | MS.gene60343:CDS | 20.0% |
| !! | CATAATATATATAAGGTTTG+AGG | + | chr8.2:19111844-19111863 | None:intergenic | 20.0% |
| !! | TTAATGATAAAACAGCTAAT+AGG | - | chr8.2:19111590-19111609 | MS.gene60343:intron | 20.0% |
| ! | ACAAAAGTCAAATCAGATTA+AGG | - | chr8.2:19111561-19111580 | MS.gene60343:intron | 25.0% |
| ! | TACATTAACTAATTAAGGTC+TGG | - | chr8.2:19111914-19111933 | MS.gene60343:CDS | 25.0% |
| ! | TATAGTTACTGTTCATAAGA+AGG | + | chr8.2:19111766-19111785 | None:intergenic | 25.0% |
| !! | GGTCTGGTTATAAATTTTAT+TGG | - | chr8.2:19111930-19111949 | MS.gene60343:CDS | 25.0% |
| !!! | ATGATTGGTTCATTATTTTC+AGG | - | chr8.2:19112071-19112090 | MS.gene60343:CDS | 25.0% |
| !!! | TTGAGTCTTTATGTTGTTTT+TGG | - | chr8.2:19111465-19111484 | MS.gene60343:intron | 25.0% |
| AAGAACATGAATATCTTCAG+TGG | - | chr8.2:19111699-19111718 | MS.gene60343:CDS | 30.0% | |
| AGATTCAATCAACATGGATT+TGG | + | chr8.2:19111677-19111696 | None:intergenic | 30.0% | |
| AGTCAAATCAGATTAAGGAA+AGG | - | chr8.2:19111566-19111585 | MS.gene60343:intron | 30.0% | |
| ! | AGATTTACCTTGTTATCTTC+AGG | + | chr8.2:19111622-19111641 | None:intergenic | 30.0% |
| !! | TAAGAGAGAGATTTGTTGAA+TGG | - | chr8.2:19112032-19112051 | MS.gene60343:CDS | 30.0% |
| !!! | TATGTTGTTTTTGGTTTCAG+AGG | - | chr8.2:19111474-19111493 | MS.gene60343:intron | 30.0% |
| !!! | TTTTGAAGTGCATAATGTTG+TGG | + | chr8.2:19111206-19111225 | None:intergenic | 30.0% |
| AACCAAATGGCAAAGAAGAA+AGG | - | chr8.2:19111110-19111129 | MS.gene60343:CDS | 35.0% | |
| AACCTTTCTTCTTTGCCATT+TGG | + | chr8.2:19111115-19111134 | None:intergenic | 35.0% | |
| AATCTGAAAGACATGAGTCA+AGG | - | chr8.2:19112100-19112119 | MS.gene60343:CDS | 35.0% | |
| ACAAAAGCAACAACAAATGG+TGG | - | chr8.2:19112123-19112142 | MS.gene60343:CDS | 35.0% | |
| AGGACAAAAGCAACAACAAA+TGG | - | chr8.2:19112120-19112139 | MS.gene60343:CDS | 35.0% | |
| GCAAAGAAGAAAGGTTGTAT+TGG | - | chr8.2:19111119-19111138 | MS.gene60343:CDS | 35.0% | |
| TACTGTTCATAAGAAGGAGT+TGG | + | chr8.2:19111760-19111779 | None:intergenic | 35.0% | |
| TCAGTGATGCTTTGTAAAGA+GGG | + | chr8.2:19112180-19112199 | None:intergenic | 35.0% | |
| TCTTGCAGATTCAATCAACA+TGG | + | chr8.2:19111683-19111702 | None:intergenic | 35.0% | |
| TTCAGTGATGCTTTGTAAAG+AGG | + | chr8.2:19112181-19112200 | None:intergenic | 35.0% | |
| TTGGTTTCAGAGGTTACAAA+GGG | - | chr8.2:19111484-19111503 | MS.gene60343:intron | 35.0% | |
| ! | TTTGGTTTCAGAGGTTACAA+AGG | - | chr8.2:19111483-19111502 | MS.gene60343:intron | 35.0% |
| !!! | TTTGACTTTTGTGCTGATTC+TGG | + | chr8.2:19111553-19111572 | None:intergenic | 35.0% |
| AGAAGAGCAACATAGTGATC+AGG | + | chr8.2:19111178-19111197 | None:intergenic | 40.0% | |
| ATCACTTCACTAGCAGTAAC+TGG | + | chr8.2:19111145-19111164 | None:intergenic | 40.0% | |
| CCTTGCTACTACAAAGTCAA+TGG | + | chr8.2:19111982-19112001 | None:intergenic | 40.0% | |
| CTTGTGATACAGCAACCAAA+TGG | - | chr8.2:19111097-19111116 | MS.gene60343:CDS | 40.0% | |
| GAAGAGCAACATAGTGATCA+GGG | + | chr8.2:19111177-19111196 | None:intergenic | 40.0% | |
| GTCTGAACCTGAAGATAACA+AGG | - | chr8.2:19111612-19111631 | MS.gene60343:intron | 40.0% | |
| TATAGACTCATCTCAGCTCA+AGG | - | chr8.2:19111349-19111368 | MS.gene60343:intron | 40.0% | |
| ! | AGAGGTTACAAAGGGTATGT+GGG | - | chr8.2:19111492-19111511 | MS.gene60343:intron | 40.0% |
| ! | CCAACAGATACACTTTTGCT+TGG | - | chr8.2:19111319-19111338 | MS.gene60343:intron | 40.0% |
| ! | CCAAGCAAAAGTGTATCTGT+TGG | + | chr8.2:19111322-19111341 | None:intergenic | 40.0% |
| !! | CCATTGACTTTGTAGTAGCA+AGG | - | chr8.2:19111979-19111998 | MS.gene60343:CDS | 40.0% |
| ! | CAGAGGTTACAAAGGGTATG+TGG | - | chr8.2:19111491-19111510 | MS.gene60343:intron | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 19111064 | 19112212 | 19111064 | ID=MS.gene60343 |
| chr8.2 | mRNA | 19111064 | 19112212 | 19111064 | ID=MS.gene60343.t1;Parent=MS.gene60343 |
| chr8.2 | exon | 19111906 | 19112212 | 19111906 | ID=MS.gene60343.t1.exon1;Parent=MS.gene60343.t1 |
| chr8.2 | CDS | 19111906 | 19112212 | 19111906 | ID=cds.MS.gene60343.t1;Parent=MS.gene60343.t1 |
| chr8.2 | exon | 19111643 | 19111782 | 19111643 | ID=MS.gene60343.t1.exon2;Parent=MS.gene60343.t1 |
| chr8.2 | CDS | 19111643 | 19111782 | 19111643 | ID=cds.MS.gene60343.t1;Parent=MS.gene60343.t1 |
| chr8.2 | exon | 19111064 | 19111183 | 19111064 | ID=MS.gene60343.t1.exon3;Parent=MS.gene60343.t1 |
| chr8.2 | CDS | 19111064 | 19111183 | 19111064 | ID=cds.MS.gene60343.t1;Parent=MS.gene60343.t1 |
| Gene Sequence |
| Protein sequence |
