Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43289.t1 | XP_003608955.1 | 93.1 | 188 | 12 | 1 | 1 | 188 | 1 | 187 | 2.10E-72 | 282 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43289.t1 | G7JNU9 | 93.1 | 188 | 12 | 1 | 1 | 188 | 1 | 187 | 1.5e-72 | 282.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051757 | MS.gene43289 | 0.823855 | 1.11E-53 | -1.69E-46 |
| MS.gene051790 | MS.gene43289 | 0.806087 | 9.75E-50 | -1.69E-46 |
| MS.gene051831 | MS.gene43289 | 0.807037 | 6.15E-50 | -1.69E-46 |
| MS.gene055603 | MS.gene43289 | 0.800709 | 1.27E-48 | -1.69E-46 |
| MS.gene055864 | MS.gene43289 | 0.813919 | 2.01E-51 | -1.69E-46 |
| MS.gene058543 | MS.gene43289 | 0.803269 | 3.78E-49 | -1.69E-46 |
| MS.gene059283 | MS.gene43289 | 0.804497 | 2.10E-49 | -1.69E-46 |
| MS.gene059774 | MS.gene43289 | 0.809076 | 2.26E-50 | -1.69E-46 |
| MS.gene060697 | MS.gene43289 | 0.807678 | 4.49E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene43289 | MS.gene074378 | PPI |
| MS.gene43289 | MS.gene022274 | PPI |
| MS.gene43289 | MS.gene25931 | PPI |
| MS.gene70603 | MS.gene43289 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43289.t1 | MTR_4g106790 | 97.340 | 188 | 4 | 1 | 1 | 188 | 1 | 187 | 3.40e-134 | 373 |
| MS.gene43289.t1 | MTR_4g106790 | 95.973 | 149 | 5 | 1 | 1 | 149 | 1 | 148 | 1.02e-96 | 278 |
| MS.gene43289.t1 | MTR_3g078300 | 54.974 | 191 | 75 | 3 | 1 | 188 | 1 | 183 | 3.05e-63 | 193 |
| MS.gene43289.t1 | MTR_3g078300 | 55.263 | 152 | 57 | 3 | 1 | 149 | 1 | 144 | 5.17e-48 | 155 |
| MS.gene43289.t1 | MTR_5g035430 | 42.328 | 189 | 87 | 4 | 1 | 186 | 1 | 170 | 8.86e-40 | 133 |
| MS.gene43289.t1 | MTR_4g097220 | 38.624 | 189 | 94 | 4 | 1 | 188 | 1 | 168 | 8.16e-35 | 120 |
| MS.gene43289.t1 | MTR_8g063200 | 38.378 | 185 | 99 | 4 | 1 | 185 | 1 | 170 | 5.27e-30 | 108 |
| MS.gene43289.t1 | MTR_4g097220 | 42.537 | 134 | 57 | 2 | 1 | 134 | 1 | 114 | 2.92e-29 | 105 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43289.t1 | AT5G62900 | 46.809 | 188 | 73 | 4 | 1 | 188 | 1 | 161 | 8.99e-48 | 153 |
| MS.gene43289.t1 | AT5G50090 | 44.444 | 189 | 73 | 4 | 1 | 188 | 1 | 158 | 6.41e-42 | 139 |
| MS.gene43289.t1 | AT5G50090 | 46.277 | 188 | 65 | 5 | 1 | 188 | 1 | 152 | 2.68e-41 | 137 |
| MS.gene43289.t1 | AT1G60010 | 41.489 | 188 | 95 | 4 | 1 | 188 | 1 | 173 | 1.22e-40 | 135 |
| MS.gene43289.t1 | AT5G50090 | 52.500 | 120 | 43 | 1 | 1 | 120 | 1 | 106 | 1.09e-35 | 122 |
| MS.gene43289.t1 | AT1G10530 | 35.638 | 188 | 99 | 3 | 1 | 188 | 1 | 166 | 8.43e-35 | 120 |
| MS.gene43289.t1 | AT5G67620 | 32.195 | 205 | 98 | 7 | 1 | 188 | 1 | 181 | 1.03e-23 | 92.8 |
Find 25 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCTTTCTTCTTTGCCATT+TGG | 0.302263 | 8.4:+20492091 | None:intergenic |
| ACAAAAGTCAAATCAGATTA+AGG | 0.303462 | 8.4:-20491642 | MS.gene43289:CDS |
| AGATTTACCTTGTTATCTTC+AGG | 0.363890 | 8.4:+20491584 | None:intergenic |
| TTAATGATAAAACAGCTAAT+AGG | 0.376595 | 8.4:-20491613 | MS.gene43289:CDS |
| ATCACTTCACTAGCAGTAAC+TGG | 0.406024 | 8.4:+20492061 | None:intergenic |
| AGAAGAGCAACATAGTGATC+AGG | 0.427746 | 8.4:+20492028 | None:intergenic |
| CAGAGGTTACAAAGGGTATG+TGG | 0.456771 | 8.4:-20491712 | MS.gene43289:CDS |
| TTCAGTGATGCTTTGTAAAG+AGG | 0.477664 | 8.4:+20491023 | None:intergenic |
| GCAAAGAAGAAAGGTTGTAT+TGG | 0.478560 | 8.4:-20492084 | MS.gene43289:CDS |
| AGGACAAAAGCAACAACAAA+TGG | 0.492369 | 8.4:-20491081 | MS.gene43289:CDS |
| TCAGTGATGCTTTGTAAAGA+GGG | 0.514695 | 8.4:+20491024 | None:intergenic |
| AACCAAATGGCAAAGAAGAA+AGG | 0.522282 | 8.4:-20492093 | MS.gene43289:CDS |
| TTGGTTTCAGAGGTTACAAA+GGG | 0.536135 | 8.4:-20491719 | MS.gene43289:intron |
| GATTCAAAACTCATATCAGC+TGG | 0.538326 | 8.4:+20490996 | None:intergenic |
| CTTGTGATACAGCAACCAAA+TGG | 0.541988 | 8.4:-20492106 | MS.gene43289:CDS |
| GAAGAGCAACATAGTGATCA+GGG | 0.575247 | 8.4:+20492029 | None:intergenic |
| CCAAGCAAAAGTGTATCTGT+TGG | 0.576593 | 8.4:+20491884 | None:intergenic |
| TTTGGTTTCAGAGGTTACAA+AGG | 0.584060 | 8.4:-20491720 | MS.gene43289:intron |
| AGAGGTTACAAAGGGTATGT+GGG | 0.585748 | 8.4:-20491711 | MS.gene43289:CDS |
| TATAGACTCATCTCAGCTCA+AGG | 0.593800 | 8.4:-20491854 | MS.gene43289:intron |
| GTCTGAACCTGAAGATAACA+AGG | 0.608807 | 8.4:-20491591 | MS.gene43289:intron |
| AGTCAAATCAGATTAAGGAA+AGG | 0.621653 | 8.4:-20491637 | MS.gene43289:CDS |
| AATCTGAAAGACATGAGTCA+AGG | 0.637514 | 8.4:-20491101 | MS.gene43289:CDS |
| ACAAAAGCAACAACAAATGG+TGG | 0.646145 | 8.4:-20491078 | MS.gene43289:CDS |
| ATAATAATAAGTCAAGAACA+TGG | 0.691937 | 8.4:-20491050 | MS.gene43289:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATTAATAATAATTTATGAT+TGG | - | chr8.4:20492005-20492024 | MS.gene43289:CDS | 10.0% |
| !! | TTATTTACATTAACTAATTA+AGG | - | chr8.4:20491858-20491877 | MS.gene43289:CDS | 10.0% |
| !! | CAATGCATAATATATATATA+AGG | + | chr8.4:20491798-20491817 | None:intergenic | 15.0% |
| !! | TAATATATATATAAGGTTTG+AGG | + | chr8.4:20491791-20491810 | None:intergenic | 15.0% |
| !! | TATAAAGAATCAAAAGAAAA+AGG | + | chr8.4:20491352-20491371 | None:intergenic | 15.0% |
| !!! | AAGAACAAAATAATCTTTTA+AGG | + | chr8.4:20491386-20491405 | None:intergenic | 15.0% |
| !!! | AGAACAAAATAATCTTTTAA+GGG | + | chr8.4:20491385-20491404 | None:intergenic | 15.0% |
| !!! | TACACATTGATTATTTTTTT+TGG | - | chr8.4:20491759-20491778 | MS.gene43289:intron | 15.0% |
| !! | ATAATAATAAGTCAAGAACA+TGG | - | chr8.4:20492100-20492119 | MS.gene43289:CDS | 20.0% |
| !! | TTAATGATAAAACAGCTAAT+AGG | - | chr8.4:20491537-20491556 | MS.gene43289:intron | 20.0% |
| ! | ACAAAAGTCAAATCAGATTA+AGG | - | chr8.4:20491508-20491527 | MS.gene43289:intron | 25.0% |
| ! | TACATTAACTAATTAAGGTC+TGG | - | chr8.4:20491863-20491882 | MS.gene43289:CDS | 25.0% |
| ! | TATAGTTACTGTTCATAAGA+AGG | + | chr8.4:20491713-20491732 | None:intergenic | 25.0% |
| !! | GGTCTGGTTATAAATTTTAT+TGG | - | chr8.4:20491879-20491898 | MS.gene43289:CDS | 25.0% |
| !!! | ATGATTGGTTCATTATTTTC+AGG | - | chr8.4:20492020-20492039 | MS.gene43289:CDS | 25.0% |
| !!! | TTGAGTCTTTATGTTGTTTT+TGG | - | chr8.4:20491412-20491431 | MS.gene43289:intron | 25.0% |
| AAGAACATGAATATCTTCAG+TGG | - | chr8.4:20491646-20491665 | MS.gene43289:CDS | 30.0% | |
| AGATTCAATCAACATGGATT+TGG | + | chr8.4:20491624-20491643 | None:intergenic | 30.0% | |
| AGTCAAATCAGATTAAGGAA+AGG | - | chr8.4:20491513-20491532 | MS.gene43289:intron | 30.0% | |
| TATCAAGTGAGTTAAGCTTA+CGG | - | chr8.4:20491822-20491841 | MS.gene43289:intron | 30.0% | |
| ! | AGATTTACCTTGTTATCTTC+AGG | + | chr8.4:20491569-20491588 | None:intergenic | 30.0% |
| !! | TAAGAGAGAGATTTGTTGAA+TGG | - | chr8.4:20491981-20492000 | MS.gene43289:CDS | 30.0% |
| !!! | TATGTTGTTTTTGGTTTCAG+AGG | - | chr8.4:20491421-20491440 | MS.gene43289:intron | 30.0% |
| !!! | TTTTGAAGTGCATAATGTTG+TGG | + | chr8.4:20491153-20491172 | None:intergenic | 30.0% |
| AACCAAATGGCAAAGAAGAA+AGG | - | chr8.4:20491057-20491076 | MS.gene43289:CDS | 35.0% | |
| AACCTTTCTTCTTTGCCATT+TGG | + | chr8.4:20491062-20491081 | None:intergenic | 35.0% | |
| AATCTGAAAGACATGAGTCA+AGG | - | chr8.4:20492049-20492068 | MS.gene43289:CDS | 35.0% | |
| ACAAAAGCAACAACAAATGG+TGG | - | chr8.4:20492072-20492091 | MS.gene43289:CDS | 35.0% | |
| AGGACAAAAGCAACAACAAA+TGG | - | chr8.4:20492069-20492088 | MS.gene43289:CDS | 35.0% | |
| GCAAAGAAGAAAGGTTGTAT+TGG | - | chr8.4:20491066-20491085 | MS.gene43289:CDS | 35.0% | |
| TACTGTTCATAAGAAGGAGT+TGG | + | chr8.4:20491707-20491726 | None:intergenic | 35.0% | |
| TCAGTGATGCTTTGTAAAGA+GGG | + | chr8.4:20492129-20492148 | None:intergenic | 35.0% | |
| TCTTGCAGATTCAATCAACA+TGG | + | chr8.4:20491630-20491649 | None:intergenic | 35.0% | |
| TTCAGTGATGCTTTGTAAAG+AGG | + | chr8.4:20492130-20492149 | None:intergenic | 35.0% | |
| TTGGTTTCAGAGGTTACAAA+GGG | - | chr8.4:20491431-20491450 | MS.gene43289:intron | 35.0% | |
| ! | TTTGGTTTCAGAGGTTACAA+AGG | - | chr8.4:20491430-20491449 | MS.gene43289:intron | 35.0% |
| !!! | TTTGACTTTTGTGCTGATTC+TGG | + | chr8.4:20491500-20491519 | None:intergenic | 35.0% |
| AGAAGAGCAACATAGTGATC+AGG | + | chr8.4:20491125-20491144 | None:intergenic | 40.0% | |
| ATCACTTCACTAGCAGTAAC+TGG | + | chr8.4:20491092-20491111 | None:intergenic | 40.0% | |
| CCTTGCTACTACAAAGTCAA+TGG | + | chr8.4:20491931-20491950 | None:intergenic | 40.0% | |
| CTTGTGATACAGCAACCAAA+TGG | - | chr8.4:20491044-20491063 | MS.gene43289:CDS | 40.0% | |
| GAAGAGCAACATAGTGATCA+GGG | + | chr8.4:20491124-20491143 | None:intergenic | 40.0% | |
| GTCTGAACCTGAAGATAACA+AGG | - | chr8.4:20491559-20491578 | MS.gene43289:intron | 40.0% | |
| TATAGACTCATCTCAGCTCA+AGG | - | chr8.4:20491296-20491315 | MS.gene43289:intron | 40.0% | |
| ! | AGAGGTTACAAAGGGTATGT+GGG | - | chr8.4:20491439-20491458 | MS.gene43289:intron | 40.0% |
| ! | CCAACAGATACACTTTTGCT+TGG | - | chr8.4:20491266-20491285 | MS.gene43289:intron | 40.0% |
| ! | CCAAGCAAAAGTGTATCTGT+TGG | + | chr8.4:20491269-20491288 | None:intergenic | 40.0% |
| !! | CCATTGACTTTGTAGTAGCA+AGG | - | chr8.4:20491928-20491947 | MS.gene43289:CDS | 40.0% |
| ! | CAGAGGTTACAAAGGGTATG+TGG | - | chr8.4:20491438-20491457 | MS.gene43289:intron | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 20491011 | 20492161 | 20491011 | ID=MS.gene43289 |
| chr8.4 | mRNA | 20491011 | 20492161 | 20491011 | ID=MS.gene43289.t1;Parent=MS.gene43289 |
| chr8.4 | exon | 20491855 | 20492161 | 20491855 | ID=MS.gene43289.t1.exon1;Parent=MS.gene43289.t1 |
| chr8.4 | CDS | 20491855 | 20492161 | 20491855 | ID=cds.MS.gene43289.t1;Parent=MS.gene43289.t1 |
| chr8.4 | exon | 20491592 | 20491731 | 20491592 | ID=MS.gene43289.t1.exon2;Parent=MS.gene43289.t1 |
| chr8.4 | CDS | 20491592 | 20491731 | 20491592 | ID=cds.MS.gene43289.t1;Parent=MS.gene43289.t1 |
| chr8.4 | exon | 20491011 | 20491130 | 20491011 | ID=MS.gene43289.t1.exon3;Parent=MS.gene43289.t1 |
| chr8.4 | CDS | 20491011 | 20491130 | 20491011 | ID=cds.MS.gene43289.t1;Parent=MS.gene43289.t1 |
| Gene Sequence |
| Protein sequence |
