AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene022830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022830.t1 MTR_8g012400 97.840 463 10 0 1 463 1 463 0.0 939
MS.gene022830.t1 MTR_8g012400 97.911 383 8 0 81 463 1 383 0.0 784
MS.gene022830.t1 MTR_7g053500 73.349 439 114 1 28 463 25 463 0.0 695
MS.gene022830.t1 MTR_7g053500 73.316 386 100 1 81 463 1 386 0.0 615
MS.gene022830.t1 MTR_4g088155 54.525 453 199 3 17 463 11 462 0.0 539
MS.gene022830.t1 MTR_5g085580 40.492 447 244 7 30 459 26 467 2.52e-118 355
MS.gene022830.t1 MTR_1g056640 38.950 457 248 5 30 459 39 491 7.66e-116 349
MS.gene022830.t1 MTR_6g088885 39.046 461 268 9 4 458 1 454 2.55e-113 343
MS.gene022830.t1 MTR_4g069940 37.719 456 274 7 10 459 9 460 2.05e-103 316
MS.gene022830.t1 MTR_3g065460 37.255 459 270 8 6 459 1 446 6.19e-100 308
MS.gene022830.t1 MTR_3g116510 38.546 454 266 8 11 458 8 454 6.47e-99 305
MS.gene022830.t1 MTR_5g078200 37.691 459 267 8 6 459 1 445 1.92e-95 301
MS.gene022830.t1 MTR_1g022160 37.143 455 255 9 30 458 25 474 3.55e-95 295
MS.gene022830.t1 MTR_1g031900 35.948 459 276 7 3 458 2 445 1.03e-93 292
MS.gene022830.t1 MTR_4g010200 40.793 353 202 5 9 358 11 359 1.49e-93 288
MS.gene022830.t1 MTR_1g031900 35.948 459 276 7 3 458 2 445 1.90e-93 292
MS.gene022830.t1 MTR_1g022160 36.703 455 257 9 30 458 25 474 6.88e-93 290
MS.gene022830.t1 MTR_5g078200 38.298 423 247 6 40 459 1 412 1.14e-92 293
MS.gene022830.t1 MTR_8g102340 35.153 458 254 9 4 458 1 418 7.16e-92 287
MS.gene022830.t1 MTR_3g083580 34.658 453 275 8 14 458 7 446 3.81e-91 286
MS.gene022830.t1 MTR_4g132280 36.721 433 260 6 30 459 46 467 4.94e-91 286
MS.gene022830.t1 MTR_1g022160 36.607 448 253 9 30 451 25 467 5.30e-91 285
MS.gene022830.t1 MTR_8g085720 33.983 462 289 8 1 459 1 449 1.78e-88 279
MS.gene022830.t1 MTR_3g080410 41.716 338 191 5 30 366 27 359 2.13e-87 271
MS.gene022830.t1 MTR_4g083500 34.298 449 269 8 30 459 45 486 1.30e-86 275
MS.gene022830.t1 MTR_7g026340 36.782 435 257 11 30 457 24 447 2.44e-85 269
MS.gene022830.t1 MTR_1g077990 43.210 324 177 5 30 352 32 349 2.49e-85 268
MS.gene022830.t1 MTR_1g007810 45.483 321 165 6 30 348 17 329 2.13e-84 264
MS.gene022830.t1 MTR_4g084480 38.920 352 206 6 3 353 2 345 1.73e-82 259
MS.gene022830.t1 MTR_4g084480 38.920 352 206 6 3 353 9 352 2.21e-82 260
MS.gene022830.t1 MTR_4g084480 38.920 352 206 6 3 353 9 352 2.28e-82 259
MS.gene022830.t1 MTR_4g052970 38.298 329 197 5 30 357 30 353 5.40e-81 256
MS.gene022830.t1 MTR_2g086530 38.095 357 209 8 8 362 10 356 1.34e-76 244
MS.gene022830.t1 MTR_2g019345 38.218 348 206 5 4 351 3 341 1.91e-76 244
MS.gene022830.t1 MTR_2g086530 38.298 329 195 6 30 357 9 330 6.57e-76 242
MS.gene022830.t1 MTR_4g114850 37.231 325 199 4 30 353 27 347 2.12e-74 238
MS.gene022830.t1 MTR_4g114850 37.231 325 199 4 30 353 27 347 2.55e-74 238
MS.gene022830.t1 MTR_4g114850 37.231 325 199 4 30 353 27 347 3.96e-74 238
MS.gene022830.t1 MTR_1g022160 39.692 325 190 5 30 352 25 345 1.36e-73 238
MS.gene022830.t1 MTR_3g116510 40.061 327 185 7 136 458 1 320 9.16e-69 222
MS.gene022830.t1 MTR_1g010120 33.548 465 273 18 12 462 10 452 2.07e-68 226
MS.gene022830.t1 MTR_2g034470 36.686 338 198 9 26 360 27 351 5.61e-68 221
MS.gene022830.t1 MTR_1g053320 32.974 464 282 17 7 459 12 457 7.41e-67 222
MS.gene022830.t1 MTR_8g092070 35.080 439 261 16 30 459 35 458 2.33e-66 223
MS.gene022830.t1 MTR_7g081350 35.159 347 211 7 1 347 1 333 3.36e-66 216
MS.gene022830.t1 MTR_2g034440 36.697 327 191 9 19 342 12 325 3.77e-66 216
MS.gene022830.t1 MTR_4g124440 34.685 444 260 17 31 462 30 455 3.34e-65 218
MS.gene022830.t1 MTR_2g034480 36.676 349 201 12 11 354 9 342 4.78e-65 214
MS.gene022830.t1 MTR_3g108600 32.543 464 268 16 7 462 13 439 8.59e-65 216
MS.gene022830.t1 MTR_3g095050 31.749 463 292 16 5 459 10 456 1.46e-64 216
MS.gene022830.t1 MTR_1g052430 36.137 321 193 6 30 349 33 342 4.77e-64 213
MS.gene022830.t1 MTR_2g034470 35.671 328 195 9 36 360 2 316 5.60e-64 210
MS.gene022830.t1 MTR_4g076255 36.923 325 184 9 30 352 26 331 1.87e-62 206
MS.gene022830.t1 MTR_2g027560 33.181 437 269 15 30 458 29 450 2.33e-61 207
MS.gene022830.t1 MTR_7g081370 34.294 347 202 7 1 347 1 321 9.90e-61 201
MS.gene022830.t1 MTR_4g112025 30.769 468 294 19 6 462 12 460 1.49e-60 206
MS.gene022830.t1 MTR_4g076470 34.203 345 205 10 6 348 1 325 2.41e-60 200
MS.gene022830.t1 MTR_4g076570 34.891 321 197 6 30 349 23 332 9.06e-59 197
MS.gene022830.t1 MTR_4g076430 34.473 351 204 12 6 352 1 329 7.24e-57 191
MS.gene022830.t1 MTR_4g076440 32.857 350 211 10 6 352 1 329 8.59e-56 188
MS.gene022830.t1 MTR_4g076490 32.923 325 200 7 30 353 98 405 1.26e-55 191
MS.gene022830.t1 MTR_4g076500 33.962 318 194 7 30 345 79 382 1.95e-54 187
MS.gene022830.t1 MTR_3g031770 29.754 447 283 17 23 459 21 446 6.17e-51 180
MS.gene022830.t1 MTR_6g032820 30.047 426 270 16 30 442 27 437 2.33e-49 174
MS.gene022830.t1 MTR_7g085390 29.638 442 280 18 30 458 27 450 1.26e-48 173
MS.gene022830.t1 MTR_5g081720 34.314 306 189 8 50 352 58 354 6.31e-47 166
MS.gene022830.t1 MTR_4g134280 32.386 352 212 16 116 458 2 336 8.74e-41 150
MS.gene022830.t1 MTR_8g063490 44.444 108 46 2 298 402 1 97 1.12e-23 95.5
MS.gene022830.t1 MTR_8g085440 47.778 90 46 1 370 459 28 116 8.21e-21 87.8
MS.gene022830.t1 MTR_1g116870 47.059 85 44 1 374 458 276 359 1.19e-20 93.6
MS.gene022830.t1 MTR_5g015720 44.318 88 48 1 372 458 327 414 2.14e-20 93.6
MS.gene022830.t1 MTR_5g097420 38.542 96 59 0 363 458 19 114 6.25e-20 85.5
MS.gene022830.t1 MTR_8g089400 38.000 100 58 1 363 458 22 121 9.32e-19 83.6
MS.gene022830.t1 MTR_8g471000 45.122 82 45 0 132 213 2 83 1.87e-18 80.5
MS.gene022830.t1 MTR_8g089400 38.000 100 58 1 363 458 42 141 2.45e-18 82.8
MS.gene022830.t1 MTR_8g085390 45.455 88 47 1 373 460 29 115 8.91e-18 81.6
MS.gene022830.t1 MTR_4g133735 38.824 85 52 0 374 458 28 112 1.30e-17 79.0
MS.gene022830.t1 MTR_8g085420 45.455 88 47 1 370 457 28 114 2.55e-17 79.0
MS.gene022830.t1 MTR_4g133730 37.647 85 53 0 374 458 28 112 6.29e-17 77.0
MS.gene022830.t1 MTR_5g091650 39.560 91 54 1 371 461 105 194 6.53e-17 79.3
MS.gene022830.t1 MTR_5g091650 39.130 92 55 1 370 461 104 194 9.15e-17 80.5
MS.gene022830.t1 MTR_4g036680 33.962 106 63 2 244 349 77 175 1.17e-15 76.3
MS.gene022830.t1 MTR_4g036775 33.962 106 63 2 244 349 77 175 1.41e-15 76.3
MS.gene022830.t1 MTR_3g082080 44.304 79 43 1 374 452 157 234 2.56e-15 75.9
MS.gene022830.t1 MTR_4g079530 38.889 90 55 0 370 459 33 122 1.01e-14 71.2
MS.gene022830.t1 MTR_3g048280 39.773 88 52 1 371 458 109 195 1.03e-14 73.2
MS.gene022830.t1 MTR_4g079530 38.462 91 56 0 370 460 33 123 1.65e-14 72.0
MS.gene022830.t1 MTR_8g085400 41.975 81 43 2 372 452 31 107 5.68e-14 68.6
MS.gene022830.t1 MTR_2g072500 34.483 87 55 1 372 458 6 90 1.75e-13 66.6
MS.gene022830.t1 MTR_2g049670 40.000 85 49 2 374 458 158 240 2.33e-13 71.2
MS.gene022830.t1 MTR_1g084820 37.647 85 51 2 370 454 416 498 4.06e-13 72.0
MS.gene022830.t1 MTR_1g084820 37.647 85 51 2 370 454 416 498 4.28e-13 71.6
MS.gene022830.t1 MTR_7g111600 36.047 86 54 1 374 459 160 244 1.44e-12 68.2
MS.gene022830.t1 MTR_8g088120 38.961 77 46 1 382 458 56 131 1.46e-12 67.4
MS.gene022830.t1 MTR_7g111600 36.047 86 54 1 374 459 160 244 1.84e-12 68.6
MS.gene022830.t1 MTR_7g111600 36.047 86 54 1 374 459 160 244 1.96e-12 68.6
MS.gene022830.t1 MTR_5g044530 34.409 93 58 2 370 460 18 109 4.46e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene022830.t1 AT3G07320 63.087 447 164 1 18 463 14 460 0.0 608
MS.gene022830.t1 AT3G23770 54.157 445 197 3 25 463 33 476 0.0 518
MS.gene022830.t1 AT4G14080 52.370 443 207 2 24 463 37 478 8.31e-180 511
MS.gene022830.t1 AT4G26830 40.741 459 260 8 6 459 1 452 4.82e-120 358
MS.gene022830.t1 AT4G26830 41.096 438 247 7 26 459 26 456 3.68e-119 357
MS.gene022830.t1 AT5G55180 40.826 436 246 7 30 459 28 457 3.32e-118 354
MS.gene022830.t1 AT2G05790 39.955 448 247 6 30 459 26 469 9.36e-117 351
MS.gene022830.t1 AT5G55180 40.647 433 245 7 26 452 24 450 7.83e-114 343
MS.gene022830.t1 AT5G56590 39.675 431 254 5 30 458 27 453 1.18e-111 339
MS.gene022830.t1 AT4G29360 36.076 474 274 7 9 458 7 475 2.36e-100 311
MS.gene022830.t1 AT4G34480 39.301 458 266 7 3 459 2 448 9.03e-100 308
MS.gene022830.t1 AT4G29360 35.744 484 272 8 9 458 7 485 2.47e-99 308
MS.gene022830.t1 AT5G24318 35.541 453 278 8 11 458 12 455 3.81e-97 300
MS.gene022830.t1 AT2G01630 36.952 433 259 6 30 459 24 445 1.39e-96 300
MS.gene022830.t1 AT2G16230 37.819 431 261 6 30 459 26 450 1.25e-94 295
MS.gene022830.t1 AT1G66250 37.037 432 257 8 30 458 33 452 2.04e-94 294
MS.gene022830.t1 AT2G01630 36.854 426 255 6 30 452 24 438 4.12e-94 291
MS.gene022830.t1 AT3G13560 36.468 436 254 7 30 458 27 446 7.07e-94 293
MS.gene022830.t1 AT3G13560 36.468 436 254 7 30 458 27 446 7.07e-94 293
MS.gene022830.t1 AT3G13560 36.468 436 254 7 30 458 27 446 7.07e-94 293
MS.gene022830.t1 AT3G13560 36.468 436 254 7 30 458 27 446 7.07e-94 293
MS.gene022830.t1 AT3G13560 36.468 436 254 7 30 458 27 446 7.07e-94 293
MS.gene022830.t1 AT5G24318 35.123 447 276 8 11 452 12 449 1.21e-92 288
MS.gene022830.t1 AT1G11820 34.873 433 268 6 30 459 45 466 4.91e-90 283
MS.gene022830.t1 AT3G15800 42.331 326 182 5 30 353 44 365 2.99e-88 275
MS.gene022830.t1 AT3G55430 35.570 447 272 10 15 458 16 449 2.38e-87 274
MS.gene022830.t1 AT1G11820 34.507 426 265 6 30 452 45 459 3.58e-86 272
MS.gene022830.t1 AT4G18340 38.109 349 209 6 5 352 11 353 2.43e-81 257
MS.gene022830.t1 AT2G27500 39.634 328 193 4 30 356 30 353 7.53e-81 255
MS.gene022830.t1 AT4G18340 38.109 349 209 6 5 352 11 353 8.26e-81 256
MS.gene022830.t1 AT1G32860 41.121 321 185 4 30 349 30 347 1.21e-80 256
MS.gene022830.t1 AT2G27500 39.634 328 193 4 30 356 30 353 1.48e-80 255
MS.gene022830.t1 AT5G42720 39.623 371 209 8 1 365 1 362 4.98e-80 255
MS.gene022830.t1 AT4G34480 40.055 362 206 6 3 361 2 355 4.01e-77 245
MS.gene022830.t1 AT1G30080 39.143 350 204 6 4 349 8 352 4.29e-77 246
MS.gene022830.t1 AT1G30080 39.143 350 204 6 4 349 8 352 1.01e-76 246
MS.gene022830.t1 AT5G24318 37.609 343 204 6 11 351 12 346 3.42e-76 243
MS.gene022830.t1 AT2G39640 34.858 459 273 14 5 459 7 443 4.79e-75 245
MS.gene022830.t1 AT2G26600 37.838 333 199 5 30 358 35 363 1.61e-74 239
MS.gene022830.t1 AT2G01630 36.388 371 222 6 30 397 24 383 2.09e-73 236
MS.gene022830.t1 AT3G57270 39.466 337 188 8 18 351 16 339 5.84e-73 234
MS.gene022830.t1 AT5G42100 38.462 325 193 6 30 351 28 348 9.95e-72 233
MS.gene022830.t1 AT5G42100 38.462 325 193 6 30 351 28 348 1.03e-71 233
MS.gene022830.t1 AT4G34480 39.535 344 198 6 117 459 3 337 5.13e-70 228
MS.gene022830.t1 AT3G61810 36.890 328 191 4 30 352 56 372 1.66e-69 226
MS.gene022830.t1 AT5G24318 35.494 324 197 6 139 458 1 316 8.19e-67 217
MS.gene022830.t1 AT4G31140 32.135 473 282 19 8 462 1 452 1.72e-64 216
MS.gene022830.t1 AT4G16260 35.530 349 209 9 6 352 1 335 3.73e-64 211
MS.gene022830.t1 AT3G46570 35.549 346 209 8 9 351 10 344 1.17e-63 210
MS.gene022830.t1 AT3G24330 31.838 468 290 17 7 462 18 468 2.14e-63 213
MS.gene022830.t1 AT2G27500 39.209 278 164 4 80 356 2 275 5.31e-63 207
MS.gene022830.t1 AT5G20340 37.580 314 183 8 30 342 44 345 1.22e-62 207
MS.gene022830.t1 AT1G11820 35.474 327 204 5 30 353 45 367 1.23e-61 206
MS.gene022830.t1 AT4G17180 33.691 466 265 18 7 458 5 440 4.56e-61 206
MS.gene022830.t1 AT3G57260 33.718 347 210 8 6 352 13 339 1.43e-60 201
MS.gene022830.t1 AT5G20390 36.499 337 198 9 6 341 13 334 1.90e-60 201
MS.gene022830.t1 AT3G57260 33.718 347 210 8 6 352 29 355 2.33e-60 201
MS.gene022830.t1 AT4G16260 35.737 319 189 9 36 352 2 306 3.32e-60 199
MS.gene022830.t1 AT5G20870 32.553 470 279 20 9 459 10 460 7.06e-59 201
MS.gene022830.t1 AT5G20330 35.783 313 188 8 30 341 35 335 1.28e-57 194
MS.gene022830.t1 AT1G77780 33.140 344 218 7 6 349 1 332 1.33e-57 194
MS.gene022830.t1 AT5G18220 31.250 464 284 19 11 459 13 456 5.67e-57 196
MS.gene022830.t1 AT2G26600 38.095 273 161 5 90 358 1 269 8.32e-57 190
MS.gene022830.t1 AT1G33220 36.364 308 177 9 30 333 35 327 4.36e-56 189
MS.gene022830.t1 AT5G58090 31.377 443 278 16 30 462 23 449 8.82e-56 192
MS.gene022830.t1 AT3G04010 30.108 465 287 21 11 459 16 458 2.30e-52 184
MS.gene022830.t1 AT3G57240 33.945 327 197 9 27 352 33 341 3.33e-52 179
MS.gene022830.t1 AT5G20560 32.647 340 211 9 9 341 8 336 2.79e-50 174
MS.gene022830.t1 AT5G64790 28.664 464 305 17 5 459 6 452 5.96e-50 177
MS.gene022830.t1 AT1G77790 30.878 353 224 9 2 349 4 341 2.04e-49 172
MS.gene022830.t1 AT3G55780 33.876 307 187 9 58 357 64 361 2.51e-47 167
MS.gene022830.t1 AT2G19440 29.783 460 295 19 11 459 3 445 7.93e-46 166
MS.gene022830.t1 AT2G19440 29.783 460 295 19 11 459 3 445 7.93e-46 166
MS.gene022830.t1 AT1G64760 29.747 474 286 22 4 459 5 449 2.74e-45 164
MS.gene022830.t1 AT1G64760 29.747 474 286 22 4 459 5 449 2.74e-45 164
MS.gene022830.t1 AT5G58480 28.182 440 283 15 30 458 27 444 2.05e-44 162
MS.gene022830.t1 AT5G67460 40.594 101 56 1 362 458 277 377 8.42e-21 94.4
MS.gene022830.t1 AT5G35740 37.113 97 60 1 363 458 19 115 2.30e-19 84.0
MS.gene022830.t1 AT2G30933 43.333 90 50 1 372 461 80 168 7.15e-19 85.9
MS.gene022830.t1 AT5G67460 41.053 95 52 1 362 452 277 371 3.25e-18 86.7
MS.gene022830.t1 AT1G66870 37.634 93 58 0 366 458 45 137 3.43e-18 81.3
MS.gene022830.t1 AT4G13600 46.067 89 47 1 374 462 61 148 3.98e-18 84.0
MS.gene022830.t1 AT1G66852 38.947 95 53 2 366 458 18 109 4.19e-18 80.1
MS.gene022830.t1 AT1G66870 37.634 93 58 0 366 458 18 110 4.89e-18 80.1
MS.gene022830.t1 AT1G79480 37.647 85 52 1 374 458 311 394 8.83e-17 82.4
MS.gene022830.t1 AT1G79480 37.647 85 52 1 374 458 310 393 9.01e-17 82.4
MS.gene022830.t1 AT4G16165 38.710 93 57 0 366 458 18 110 1.90e-16 75.5
MS.gene022830.t1 AT2G04910 37.500 88 55 0 374 461 15 102 2.40e-16 75.1
MS.gene022830.t1 AT4G05430 35.922 103 66 0 356 458 5 107 2.76e-16 76.3
MS.gene022830.t1 AT4G05430 35.922 103 66 0 356 458 30 132 5.25e-16 76.3
MS.gene022830.t1 AT1G66855 35.227 88 57 0 371 458 23 110 5.57e-16 74.3
MS.gene022830.t1 AT2G30933 43.210 81 45 1 372 452 80 159 1.81e-15 74.3
MS.gene022830.t1 AT2G30933 43.210 81 45 1 372 452 80 159 1.81e-15 74.3
MS.gene022830.t1 AT5G61130 37.363 91 56 1 369 459 16 105 3.11e-15 74.7
MS.gene022830.t1 AT1G29380 41.176 85 49 1 374 458 148 231 4.07e-15 76.6
MS.gene022830.t1 AT1G29380 41.176 85 49 1 374 458 148 231 4.64e-15 75.5
MS.gene022830.t1 AT1G18650 41.860 86 49 1 374 459 21 105 6.27e-15 73.6
MS.gene022830.t1 AT2G04910 37.975 79 49 0 374 452 15 93 1.57e-14 70.5
MS.gene022830.t1 AT1G18650 41.860 86 49 1 374 459 21 105 2.08e-14 70.5
MS.gene022830.t1 AT5G63240 30.435 115 77 2 346 458 13 126 6.04e-14 68.9
MS.gene022830.t1 AT3G58100 36.782 87 55 0 374 460 41 127 9.83e-14 69.7
MS.gene022830.t1 AT1G09460 40.449 89 50 2 370 458 133 218 1.00e-13 72.4
MS.gene022830.t1 AT4G05430 34.021 97 64 0 356 452 30 126 1.34e-13 68.2
MS.gene022830.t1 AT3G58100 36.782 87 55 0 374 460 117 203 1.40e-13 70.9
MS.gene022830.t1 AT5G63225 34.483 87 56 1 372 458 23 108 2.75e-13 66.6
MS.gene022830.t1 AT4G09462 35.632 87 55 1 372 458 27 112 3.29e-13 66.6
MS.gene022830.t1 AT5G63250 32.673 101 65 2 358 456 27 126 4.07e-13 66.6
MS.gene022830.t1 AT1G29380 40.506 79 46 1 374 452 148 225 6.65e-13 68.6
MS.gene022830.t1 AT1G29380 40.506 79 46 1 374 452 148 225 6.65e-13 68.6
MS.gene022830.t1 AT4G09465 34.483 87 56 1 372 458 29 114 8.98e-13 65.5
MS.gene022830.t1 AT4G09467 34.483 87 56 1 372 458 29 114 1.03e-12 65.1
MS.gene022830.t1 AT4G09464 34.483 87 56 1 372 458 29 114 1.03e-12 65.1
MS.gene022830.t1 AT5G08000 37.079 89 55 1 371 459 37 124 2.12e-12 66.6
MS.gene022830.t1 AT2G43670 35.870 92 55 3 368 458 29 117 6.18e-12 63.2
MS.gene022830.t1 AT1G69295 36.782 87 53 2 369 455 16 100 2.40e-11 63.9
MS.gene022830.t1 AT2G29735 35.165 91 56 2 370 458 27 116 2.45e-11 61.2
MS.gene022830.t1 AT5G08000 36.782 87 54 1 373 459 20 105 2.91e-11 63.2
MS.gene022830.t1 AT4G09466 32.184 87 58 1 372 458 29 114 3.93e-11 60.5

Find 117 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTATGCGTTTAGTTCTTAC+TGG 0.231015 8.1:-84699238 MS.gene022830:CDS
ATTTACGAGATTGACCTTTC+TGG 0.245495 8.1:-84699464 MS.gene022830:CDS
CTTACTTGATATAGTGAATA+AGG 0.302923 8.1:+84701220 None:intergenic
TACTAATTTGTTTGATCAAA+TGG 0.305685 8.1:-84699783 MS.gene022830:CDS
AACATCACGGTTACAGATTC+TGG 0.306607 8.1:-84699821 MS.gene022830:CDS
GTTCTTACTGGGCTCAGTTT+AGG 0.306807 8.1:-84699226 MS.gene022830:CDS
TTCGAACCTGATTCAGTTGT+TGG 0.322029 8.1:-84699269 MS.gene022830:CDS
ACAATGCTGGTACATATAAT+AGG 0.323421 8.1:-84699646 MS.gene022830:CDS
TGAGAACTTGAAACCGGGTT+TGG 0.333691 8.1:-84699531 MS.gene022830:CDS
CGTAAATACTCGACCCATTT+GGG 0.335947 8.1:+84699480 None:intergenic
CCGCCACCAGATGATTATAA+AGG 0.339804 8.1:-84699410 MS.gene022830:CDS
CCCATTGATTTGCAAGTGTT+TGG 0.339997 8.1:+84700221 None:intergenic
TACATTTGTTACAAGTTCAT+TGG 0.343644 8.1:+84700252 None:intergenic
GCACAACCAGGAGTGAATTA+TGG 0.344738 8.1:-84700421 MS.gene022830:CDS
CTCATTCGTTATCTTCTTGT+TGG 0.349321 8.1:-84700163 MS.gene022830:CDS
CTTCATCAAAGAATCACATT+TGG 0.364686 8.1:+84698934 None:intergenic
ATCTGTAACCGTGATGTTAT+CGG 0.375981 8.1:+84699826 None:intergenic
AACTGAATCAGGTTCGAAAC+AGG 0.376600 8.1:+84699274 None:intergenic
TAATCCAAGTTAATATTAAT+AGG 0.377635 8.1:+84699863 None:intergenic
AATGGAGCGGCTTGGGTATC+CGG 0.378158 8.1:-84699741 MS.gene022830:CDS
TACAGATTCTGGTACGGGTT+TGG 0.381587 8.1:-84699810 MS.gene022830:CDS
GTACAATGTGGGGCCATGTT+TGG 0.383285 8.1:+84700113 None:intergenic
ACATTTGTTACAAGTTCATT+GGG 0.388586 8.1:+84700253 None:intergenic
GCCTTCCCTTTATAATCATC+TGG 0.397540 8.1:+84699404 None:intergenic
TTCGGGTTATTGTACCCAAA+TGG 0.398442 8.1:-84699494 MS.gene022830:CDS
GTATAATGAGAACTTGAAAC+CGG 0.400133 8.1:-84699537 MS.gene022830:CDS
TCGTAAATACTCGACCCATT+TGG 0.407207 8.1:+84699479 None:intergenic
TTCATCAAAGAATCACATTT+GGG 0.408172 8.1:+84698935 None:intergenic
GGCGTGGTGTGTGGTGGCAG+AGG 0.410468 8.1:-84699384 MS.gene022830:CDS
ACCAAACACTTGCAAATCAA+TGG 0.422374 8.1:-84700222 MS.gene022830:CDS
CCATGTTTGGTTTGTTGTTG+AGG 0.425009 8.1:+84700126 None:intergenic
GAGAACTTGAAACCGGGTTT+GGG 0.425466 8.1:-84699530 MS.gene022830:CDS
CAGATATTTATCGCTGAAAC+CGG 0.425511 8.1:-84699713 MS.gene022830:CDS
TTTCTAAATGTGCCATTTGA+AGG 0.426130 8.1:+84699989 None:intergenic
AGTCAAAGTTGGAACACCTT+TGG 0.427478 8.1:-84700041 MS.gene022830:CDS
AGAAAGGTACGAGGTTGGTT+TGG 0.432633 8.1:+84700197 None:intergenic
TTGGCCTAATGATGGTGATT+TGG 0.439274 8.1:-84699690 MS.gene022830:CDS
ATCACCATCATTAGGCCAAC+CGG 0.440869 8.1:+84699694 None:intergenic
GCGATAAATATCTGAATATC+CGG 0.441239 8.1:+84699722 None:intergenic
AGATCCTATTAATATTAACT+TGG 0.441837 8.1:-84699867 MS.gene022830:CDS
GCATGACCAACAACTGAATC+AGG 0.445045 8.1:+84699263 None:intergenic
TGGGTACGGAGCGTCACTTC+GGG 0.445726 8.1:-84699511 MS.gene022830:CDS
TCGAGGGCTTTGAGGATTTG+TGG 0.445943 8.1:+84700307 None:intergenic
AGCAAACAAGACGGCGGTAG+TGG 0.452697 8.1:-84699360 MS.gene022830:CDS
CGCCACCAGATGATTATAAA+GGG 0.453883 8.1:-84699409 MS.gene022830:CDS
GATACTGAGGTTGTTGGTGT+TGG 0.457406 8.1:+84700379 None:intergenic
GGGGAACCGAACATGTGAAT+TGG 0.459727 8.1:-84699312 MS.gene022830:CDS
GTTGAACCAATTCACATGTT+CGG 0.460183 8.1:+84699306 None:intergenic
TGGTCCAAATCACCATCATT+AGG 0.461603 8.1:+84699686 None:intergenic
CTATGCGTTTAGTTCTTACT+GGG 0.467912 8.1:-84699237 MS.gene022830:CDS
GGTTTGCATAAAGTCAAAGT+TGG 0.468564 8.1:-84700052 MS.gene022830:CDS
ACATCATTTCCACCTTCAAA+TGG 0.475381 8.1:-84700001 MS.gene022830:CDS
TGGGGAGCATGATGGATACT+TGG 0.476577 8.1:+84700272 None:intergenic
TGAGTAGAAAGGTACGAGGT+TGG 0.478386 8.1:+84700192 None:intergenic
TAATCATCTGGTGGCGGCAA+TGG 0.481167 8.1:+84699416 None:intergenic
TCGGGTTATTGTACCCAAAT+GGG 0.481245 8.1:-84699493 MS.gene022830:CDS
GGTCGAGCCGGTGTCCCAAC+CGG 0.484590 8.1:+84699593 None:intergenic
ATTTGCAAGTGTTTGGTTTG+AGG 0.484955 8.1:+84700228 None:intergenic
AGTTGTCCATAATTCACTCC+TGG 0.489434 8.1:+84700415 None:intergenic
ATGAGAGATACTGAGGTTGT+TGG 0.492536 8.1:+84700373 None:intergenic
ACTTCGGCGTCTTTCCAGAA+AGG 0.492821 8.1:+84699450 None:intergenic
TTGGGTACGGAGCGTCACTT+CGG 0.498275 8.1:-84699512 MS.gene022830:CDS
TGGGCTCAGTTTAGGAGTGT+AGG 0.499642 8.1:-84699218 MS.gene022830:CDS
ACGGTTACAGATTCTGGTAC+GGG 0.503195 8.1:-84699815 MS.gene022830:CDS
TTGTCATATGCTTGCTCACA+GGG 0.505455 8.1:-84699332 MS.gene022830:CDS
AGTTGGAACACCTTTGGCAA+TGG 0.505469 8.1:-84700035 MS.gene022830:CDS
AAAACCTCCGGTTGGGACAC+CGG 0.507755 8.1:-84699600 MS.gene022830:CDS
CCAAACACTTGCAAATCAAT+GGG 0.509879 8.1:-84700221 MS.gene022830:CDS
GGTGGGACTTGCAACTTCAA+TGG 0.512252 8.1:-84699197 MS.gene022830:CDS
CACGGTTACAGATTCTGGTA+CGG 0.519293 8.1:-84699816 MS.gene022830:CDS
TGTGAATATTCGCACCAACT+TGG 0.523084 8.1:+84699666 None:intergenic
AAGTTCATTGGGGAGCATGA+TGG 0.523087 8.1:+84700264 None:intergenic
CTTGAAACCGGGTTTGGGTA+CGG 0.532871 8.1:-84699525 MS.gene022830:CDS
CTCAAAGCCCTCGAAAACAC+TGG 0.533455 8.1:-84700298 MS.gene022830:CDS
AGAATTGAACCTGGTCGAGC+CGG 0.537030 8.1:+84699581 None:intergenic
CTCAATGAAAGCAATAGCTA+TGG 0.542784 8.1:-84701270 None:intergenic
GGAGTGTTTCATTCTGTACA+TGG 0.546711 8.1:+84700087 None:intergenic
GCTCAGTTTAGGAGTGTAGG+TGG 0.550053 8.1:-84699215 MS.gene022830:CDS
TATTTATCGCTGAAACCGGT+TGG 0.550062 8.1:-84699709 MS.gene022830:CDS
TGTTTCATTCTGTACATGGC+AGG 0.550842 8.1:+84700091 None:intergenic
CCTTTATAATCATCTGGTGG+CGG 0.551740 8.1:+84699410 None:intergenic
GCGTGGTGTGTGGTGGCAGA+GGG 0.564565 8.1:-84699383 MS.gene022830:CDS
GGTGCGAATATTCACAATGC+TGG 0.565787 8.1:-84699659 MS.gene022830:CDS
TACAAAGCAAATATAAAAGA+CGG 0.575113 8.1:+84699557 None:intergenic
GTTGGGACACCGGCTCGACC+AGG 0.576342 8.1:-84699590 MS.gene022830:CDS
GTTTGATCAAATGGTGGATG+CGG 0.578822 8.1:-84699774 MS.gene022830:CDS
CTAAATGTGCCATTTGAAGG+TGG 0.580832 8.1:+84699992 None:intergenic
CCTCAACAACAAACCAAACA+TGG 0.582013 8.1:-84700126 MS.gene022830:CDS
ATTGTCATATGCTTGCTCAC+AGG 0.586674 8.1:-84699333 MS.gene022830:CDS
TGTAAAACATCCATTGCCAA+AGG 0.589625 8.1:+84700025 None:intergenic
CTCAGTTTAGGAGTGTAGGT+GGG 0.590399 8.1:-84699214 MS.gene022830:CDS
ACCAGATGATTATAAAGGGA+AGG 0.595170 8.1:-84699405 MS.gene022830:CDS
TAAAGGGAAGGCGTGGTGTG+TGG 0.595905 8.1:-84699393 MS.gene022830:CDS
AATGGCTTAAGCTCTGACTT+CGG 0.599216 8.1:+84699434 None:intergenic
TTCCCTTTATAATCATCTGG+TGG 0.608747 8.1:+84699407 None:intergenic
ACTGAATCAGGTTCGAAACA+GGG 0.609146 8.1:+84699275 None:intergenic
TGTACATGGCAGGTACAATG+TGG 0.609170 8.1:+84700101 None:intergenic
TAATTTGTTTGATCAAATGG+TGG 0.609839 8.1:-84699780 MS.gene022830:CDS
CGAGCCGGTGTCCCAACCGG+AGG 0.610594 8.1:+84699596 None:intergenic
AGGGAAGGCGTGGTGTGTGG+TGG 0.610621 8.1:-84699390 MS.gene022830:CDS
GCAGAAATCTCAGCACAACC+AGG 0.611520 8.1:-84700433 MS.gene022830:CDS
GGAGTGAATTATGGACAACT+AGG 0.612181 8.1:-84700412 MS.gene022830:CDS
TATAATGAGAACTTGAAACC+GGG 0.614265 8.1:-84699536 MS.gene022830:CDS
TGGATCCTCTGCAATTTGAG+TGG 0.615021 8.1:+84699169 None:intergenic
GTACATGGCAGGTACAATGT+GGG 0.617161 8.1:+84700102 None:intergenic
TGACGCTCCGTACCCAAACC+CGG 0.623422 8.1:+84699518 None:intergenic
GATGGTGATTTGGACCAAGT+TGG 0.630366 8.1:-84699680 MS.gene022830:CDS
GAAACCGGTTGGCCTAATGA+TGG 0.634825 8.1:-84699698 MS.gene022830:CDS
ACATGTGAATTGGTTCAACC+CGG 0.637557 8.1:-84699302 MS.gene022830:CDS
GGCAGAGGGAGCAAACAAGA+CGG 0.641592 8.1:-84699369 MS.gene022830:CDS
ATGATTATAAAGGGAAGGCG+TGG 0.641910 8.1:-84699400 MS.gene022830:CDS
TACATGGCAGGTACAATGTG+GGG 0.663864 8.1:+84700103 None:intergenic
CATTTGTTACAAGTTCATTG+GGG 0.666428 8.1:+84700254 None:intergenic
AAAGACGGAAGAATTGAACC+TGG 0.673862 8.1:+84699572 None:intergenic
TCTTGCCACTCAAATTGCAG+AGG 0.690039 8.1:-84699174 MS.gene022830:CDS
AGAGGGAGCAAACAAGACGG+CGG 0.740507 8.1:-84699366 MS.gene022830:CDS
TGTCATATGCTTGCTCACAG+GGG 0.741994 8.1:-84699331 MS.gene022830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTCAATTTTTGAAATAT+TGG - chr8.1:84699170-84699189 MS.gene022830:CDS 10.0%
!! AATTAAAAAAACAACTTACT+TGG + chr8.1:84701059-84701078 None:intergenic 15.0%
!! TAATCCAAGTTAATATTAAT+AGG + chr8.1:84700345-84700364 None:intergenic 15.0%
!! TTAAAATCAAAACATGATTA+TGG + chr8.1:84701156-84701175 None:intergenic 15.0%
!! TTAAAATCTAAATGAATGTT+TGG + chr8.1:84699312-84699331 None:intergenic 15.0%
!!! ATATAATCATTCAGTTTTTA+TGG + chr8.1:84701112-84701131 None:intergenic 15.0%
!! AACTACGTTAATCATATAAA+AGG + chr8.1:84699561-84699580 None:intergenic 20.0%
!! AAGAAAAAAAACTTGAAACA+TGG - chr8.1:84699482-84699501 MS.gene022830:CDS 20.0%
!! AATGAAAATGTACAAAGTAT+CGG + chr8.1:84701224-84701243 None:intergenic 20.0%
!! AGATCCTATTAATATTAACT+TGG - chr8.1:84700338-84700357 MS.gene022830:CDS 20.0%
!! TACAAAGCAAATATAAAAGA+CGG + chr8.1:84700651-84700670 None:intergenic 20.0%
!! TATCATGTTCTAATATATAG+TGG + chr8.1:84699674-84699693 None:intergenic 20.0%
!! TTTCTAACGAAATAACAATA+TGG - chr8.1:84699333-84699352 MS.gene022830:CDS 20.0%
!! TTTGATTGATGTAGTAAATA+AGG + chr8.1:84699038-84699057 None:intergenic 20.0%
!!! ATTTTCATTTTTGAAGGTTA+TGG - chr8.1:84701235-84701254 MS.gene022830:CDS 20.0%
!!! TAAATGAATGTTTGGTTTTA+GGG + chr8.1:84699304-84699323 None:intergenic 20.0%
!!! TACTAATTTGTTTGATCAAA+TGG - chr8.1:84700422-84700441 MS.gene022830:CDS 20.0%
!!! TTGTACATTTTCATTTTTGA+AGG - chr8.1:84701229-84701248 MS.gene022830:CDS 20.0%
! AAACACAGATTAAATAGTAG+TGG + chr8.1:84699233-84699252 None:intergenic 25.0%
! AAATGAATAAGTCTCATCAT+TGG - chr8.1:84699417-84699436 MS.gene022830:CDS 25.0%
! AACACAGATTAAATAGTAGT+GGG + chr8.1:84699232-84699251 None:intergenic 25.0%
! ACATGTAAAAACTCTAACAT+TGG + chr8.1:84700267-84700286 None:intergenic 25.0%
! ATGCAAACCAAAAATAGTTA+AGG + chr8.1:84700142-84700161 None:intergenic 25.0%
! CTTACTTGATATAGTGAATA+AGG + chr8.1:84698988-84699007 None:intergenic 25.0%
! GGTCAAACATAAAAACAAAA+GGG + chr8.1:84701203-84701222 None:intergenic 25.0%
!! AAACACTCCTTAACTATTTT+TGG - chr8.1:84700132-84700151 MS.gene022830:CDS 25.0%
!! ACATTTGTTACAAGTTCATT+GGG + chr8.1:84699955-84699974 None:intergenic 25.0%
!! TACATTTGTTACAAGTTCAT+TGG + chr8.1:84699956-84699975 None:intergenic 25.0%
!!! CTAAATGAATGTTTGGTTTT+AGG + chr8.1:84699305-84699324 None:intergenic 25.0%
!!! TAATTTGTTTGATCAAATGG+TGG - chr8.1:84700425-84700444 MS.gene022830:CDS 25.0%
AAAAGTTACGAAAAAACCTC+CGG - chr8.1:84700593-84700612 MS.gene022830:intron 30.0%
AAGGAGAAGATAAATAGAGA+AGG + chr8.1:84699019-84699038 None:intergenic 30.0%
ACAATGCTGGTACATATAAT+AGG - chr8.1:84700559-84700578 MS.gene022830:intron 30.0%
ATAAAACACTAGAGAAGAAG+AGG - chr8.1:84701130-84701149 MS.gene022830:intron 30.0%
CGGTCAAACATAAAAACAAA+AGG + chr8.1:84701204-84701223 None:intergenic 30.0%
CTATGTGTACAATTGAAGTA+GGG - chr8.1:84699362-84699381 MS.gene022830:CDS 30.0%
GCGATAAATATCTGAATATC+CGG + chr8.1:84700486-84700505 None:intergenic 30.0%
GTATAATGAGAACTTGAAAC+CGG - chr8.1:84700668-84700687 MS.gene022830:intron 30.0%
TATAATGAGAACTTGAAACC+GGG - chr8.1:84700669-84700688 MS.gene022830:intron 30.0%
TATGTGTACAATTGAAGTAG+GGG - chr8.1:84699363-84699382 MS.gene022830:CDS 30.0%
TCTATGTGTACAATTGAAGT+AGG - chr8.1:84699361-84699380 MS.gene022830:CDS 30.0%
! CATTTGTTACAAGTTCATTG+GGG + chr8.1:84699954-84699973 None:intergenic 30.0%
!! ATCATAGATTTTGACACGTT+TGG + chr8.1:84699872-84699891 None:intergenic 30.0%
!! GGATATATTGATTCAATGGT+GGG - chr8.1:84699458-84699477 MS.gene022830:CDS 30.0%
!! TGTGGGATATATTGATTCAA+TGG - chr8.1:84699454-84699473 MS.gene022830:CDS 30.0%
!! TTTCTAAATGTGCCATTTGA+AGG + chr8.1:84700219-84700238 None:intergenic 30.0%
!!! ATGAGTGTTTTTGAGTAGAA+AGG + chr8.1:84700027-84700046 None:intergenic 30.0%
!!! ATGTGTATCCATTTTTTGCT+TGG - chr8.1:84700310-84700329 MS.gene022830:CDS 30.0%
ACATCATTTCCACCTTCAAA+TGG - chr8.1:84700204-84700223 MS.gene022830:CDS 35.0%
ACCAAACACTTGCAAATCAA+TGG - chr8.1:84699983-84700002 MS.gene022830:CDS 35.0%
ACCAGATGATTATAAAGGGA+AGG - chr8.1:84700800-84700819 MS.gene022830:intron 35.0%
ATCTGTAACCGTGATGTTAT+CGG + chr8.1:84700382-84700401 None:intergenic 35.0%
ATTTACGAGATTGACCTTTC+TGG - chr8.1:84700741-84700760 MS.gene022830:intron 35.0%
CAGATATTTATCGCTGAAAC+CGG - chr8.1:84700492-84700511 MS.gene022830:intron 35.0%
CCAAACACTTGCAAATCAAT+GGG - chr8.1:84699984-84700003 MS.gene022830:CDS 35.0%
CTCATTCGTTATCTTCTTGT+TGG - chr8.1:84700042-84700061 MS.gene022830:CDS 35.0%
GGTTTGCATAAAGTCAAAGT+TGG - chr8.1:84700153-84700172 MS.gene022830:CDS 35.0%
GTTGAACCAATTCACATGTT+CGG + chr8.1:84700902-84700921 None:intergenic 35.0%
GTTGAGCTTGATTGCATATT+TGG - chr8.1:84699194-84699213 MS.gene022830:CDS 35.0%
TCATCATGAGATAGCTAAGA+TGG - chr8.1:84699144-84699163 MS.gene022830:intron 35.0%
TGTAAAACATCCATTGCCAA+AGG + chr8.1:84700183-84700202 None:intergenic 35.0%
TTCCCTTTATAATCATCTGG+TGG + chr8.1:84700801-84700820 None:intergenic 35.0%
! AGGTTAGAAAATTGGGTTAG+AGG + chr8.1:84699740-84699759 None:intergenic 35.0%
! GTTTTTGATGAGAGATACTG+AGG + chr8.1:84699842-84699861 None:intergenic 35.0%
! TCTGAAGTCCAAGCAAAAAA+TGG + chr8.1:84700321-84700340 None:intergenic 35.0%
! TGGATCAAGTGAGATTTTGT+GGG - chr8.1:84699437-84699456 MS.gene022830:CDS 35.0%
! TTGGATCAAGTGAGATTTTG+TGG - chr8.1:84699436-84699455 MS.gene022830:CDS 35.0%
!! ATTTGCAAGTGTTTGGTTTG+AGG + chr8.1:84699980-84699999 None:intergenic 35.0%
!! CTATGCGTTTAGTTCTTACT+GGG - chr8.1:84700968-84700987 MS.gene022830:intron 35.0%
!! GGGATATATTGATTCAATGG+TGG - chr8.1:84699457-84699476 MS.gene022830:CDS 35.0%
!! TTTTGAGTCCGATAACATCA+CGG - chr8.1:84700371-84700390 MS.gene022830:CDS 35.0%
!!! TTTTTGAGTAGAAAGGTACG+AGG + chr8.1:84700020-84700039 None:intergenic 35.0%
AAAGACGGAAGAATTGAACC+TGG + chr8.1:84700636-84700655 None:intergenic 40.0%
AACATCACGGTTACAGATTC+TGG - chr8.1:84700384-84700403 MS.gene022830:CDS 40.0%
AACCAACAATCTGAGCTTGA+TGG - chr8.1:84699256-84699275 MS.gene022830:CDS 40.0%
AACTGAATCAGGTTCGAAAC+AGG + chr8.1:84700934-84700953 None:intergenic 40.0%
ACTGAATCAGGTTCGAAACA+GGG + chr8.1:84700933-84700952 None:intergenic 40.0%
AGTCAAAGTTGGAACACCTT+TGG - chr8.1:84700164-84700183 MS.gene022830:CDS 40.0%
AGTTGTCCATAATTCACTCC+TGG + chr8.1:84699793-84699812 None:intergenic 40.0%
ATGAGAGATACTGAGGTTGT+TGG + chr8.1:84699835-84699854 None:intergenic 40.0%
ATGATTATAAAGGGAAGGCG+TGG - chr8.1:84700805-84700824 MS.gene022830:intron 40.0%
ATTGTCATATGCTTGCTCAC+AGG - chr8.1:84700872-84700891 MS.gene022830:intron 40.0%
CCTCAACAACAAACCAAACA+TGG - chr8.1:84700079-84700098 MS.gene022830:CDS 40.0%
CCTTTATAATCATCTGGTGG+CGG + chr8.1:84700798-84700817 None:intergenic 40.0%
CGCCACCAGATGATTATAAA+GGG - chr8.1:84700796-84700815 MS.gene022830:intron 40.0%
CGTAAATACTCGACCCATTT+GGG + chr8.1:84700728-84700747 None:intergenic 40.0%
GCCTTCCCTTTATAATCATC+TGG + chr8.1:84700804-84700823 None:intergenic 40.0%
GGAGTGAATTATGGACAACT+AGG - chr8.1:84699793-84699812 MS.gene022830:CDS 40.0%
TATTTATCGCTGAAACCGGT+TGG - chr8.1:84700496-84700515 MS.gene022830:intron 40.0%
TCGGGTTATTGTACCCAAAT+GGG - chr8.1:84700712-84700731 MS.gene022830:intron 40.0%
TCGTAAATACTCGACCCATT+TGG + chr8.1:84700729-84700748 None:intergenic 40.0%
TGGTCCAAATCACCATCATT+AGG + chr8.1:84700522-84700541 None:intergenic 40.0%
TGGTGGCAGGTTAGAAAATT+GGG + chr8.1:84699747-84699766 None:intergenic 40.0%
TGTGAATATTCGCACCAACT+TGG + chr8.1:84700542-84700561 None:intergenic 40.0%
TGTTTCATTCTGTACATGGC+AGG + chr8.1:84700117-84700136 None:intergenic 40.0%
TTACGAAAAAACCTCCGGTT+GGG - chr8.1:84700598-84700617 MS.gene022830:intron 40.0%
TTCGAACCTGATTCAGTTGT+TGG - chr8.1:84700936-84700955 MS.gene022830:intron 40.0%
TTGTCATATGCTTGCTCACA+GGG - chr8.1:84700873-84700892 MS.gene022830:intron 40.0%
! AATGGCTTAAGCTCTGACTT+CGG + chr8.1:84700774-84700793 None:intergenic 40.0%
! ACATGTGAATTGGTTCAACC+CGG - chr8.1:84700903-84700922 MS.gene022830:intron 40.0%
! GGAGTGTTTCATTCTGTACA+TGG + chr8.1:84700121-84700140 None:intergenic 40.0%
! TTCGGGTTATTGTACCCAAA+TGG - chr8.1:84700711-84700730 MS.gene022830:intron 40.0%
! TTGGATTCCAGTGTTTTCGA+GGG + chr8.1:84699917-84699936 None:intergenic 40.0%
! TTGGCCTAATGATGGTGATT+TGG - chr8.1:84700515-84700534 MS.gene022830:intron 40.0%
!! CCATGTTTGGTTTGTTGTTG+AGG + chr8.1:84700082-84700101 None:intergenic 40.0%
!! CCCATTGATTTGCAAGTGTT+TGG + chr8.1:84699987-84700006 None:intergenic 40.0%
!! CTAAATGTGCCATTTGAAGG+TGG + chr8.1:84700216-84700235 None:intergenic 40.0%
!! GCTATGCGTTTAGTTCTTAC+TGG - chr8.1:84700967-84700986 MS.gene022830:intron 40.0%
!! GTTTGATCAAATGGTGGATG+CGG - chr8.1:84700431-84700450 MS.gene022830:CDS 40.0%
!!! GTTTGGTTTTAGGGTGAAAC+TGG + chr8.1:84699295-84699314 None:intergenic 40.0%
!!! TTTTGACACGTTTGGCTTTG+AGG + chr8.1:84699864-84699883 None:intergenic 40.0%
ACGGTTACAGATTCTGGTAC+GGG - chr8.1:84700390-84700409 MS.gene022830:CDS 45.0%
AGTTGGAACACCTTTGGCAA+TGG - chr8.1:84700170-84700189 MS.gene022830:CDS 45.0%
ATCACCATCATTAGGCCAAC+CGG + chr8.1:84700514-84700533 None:intergenic 45.0%
CACGGTTACAGATTCTGGTA+CGG - chr8.1:84700389-84700408 MS.gene022830:CDS 45.0%
CCGCCACCAGATGATTATAA+AGG - chr8.1:84700795-84700814 MS.gene022830:intron 45.0%
GAGAACTTGAAACCGGGTTT+GGG - chr8.1:84700675-84700694 MS.gene022830:intron 45.0%
GATACTGAGGTTGTTGGTGT+TGG + chr8.1:84699829-84699848 None:intergenic 45.0%
GCACAACCAGGAGTGAATTA+TGG - chr8.1:84699784-84699803 MS.gene022830:CDS 45.0%
GCATGACCAACAACTGAATC+AGG + chr8.1:84700945-84700964 None:intergenic 45.0%
GGTGCGAATATTCACAATGC+TGG - chr8.1:84700546-84700565 MS.gene022830:intron 45.0%
GTACATGGCAGGTACAATGT+GGG + chr8.1:84700106-84700125 None:intergenic 45.0%
GTGGTGGCAGGTTAGAAAAT+TGG + chr8.1:84699748-84699767 None:intergenic 45.0%
GTTACGAAAAAACCTCCGGT+TGG - chr8.1:84700597-84700616 MS.gene022830:intron 45.0%
GTTCTTACTGGGCTCAGTTT+AGG - chr8.1:84700979-84700998 MS.gene022830:intron 45.0%
TACAGATTCTGGTACGGGTT+TGG - chr8.1:84700395-84700414 MS.gene022830:CDS 45.0%
TACATGGCAGGTACAATGTG+GGG + chr8.1:84700105-84700124 None:intergenic 45.0%
TCTTGCCACTCAAATTGCAG+AGG - chr8.1:84701031-84701050 MS.gene022830:intron 45.0%
TGAGAACTTGAAACCGGGTT+TGG - chr8.1:84700674-84700693 MS.gene022830:intron 45.0%
TGCCATCAAGCTCAGATTGT+TGG + chr8.1:84699261-84699280 None:intergenic 45.0%
TGGATCCTCTGCAATTTGAG+TGG + chr8.1:84701039-84701058 None:intergenic 45.0%
TGTACATGGCAGGTACAATG+TGG + chr8.1:84700107-84700126 None:intergenic 45.0%
TGTCATATGCTTGCTCACAG+GGG - chr8.1:84700874-84700893 MS.gene022830:intron 45.0%
! AAGTTCATTGGGGAGCATGA+TGG + chr8.1:84699944-84699963 None:intergenic 45.0%
! AGAAAGGTACGAGGTTGGTT+TGG + chr8.1:84700011-84700030 None:intergenic 45.0%
! AGGTTCGAAACAGGGTTTTC+CGG + chr8.1:84700925-84700944 None:intergenic 45.0%
! ATTTTCTAACCTGCCACCAC+AGG - chr8.1:84699748-84699767 MS.gene022830:CDS 45.0%
! ATTTTGCAATGGAGCGGCTT+GGG - chr8.1:84700457-84700476 MS.gene022830:intron 45.0%
! CGGTGTATTTTGCAATGGAG+CGG - chr8.1:84700451-84700470 MS.gene022830:intron 45.0%
! CTCAGTTTAGGAGTGTAGGT+GGG - chr8.1:84700991-84701010 MS.gene022830:intron 45.0%
! CTTGGATTCCAGTGTTTTCG+AGG + chr8.1:84699918-84699937 None:intergenic 45.0%
! GATGGTGATTTGGACCAAGT+TGG - chr8.1:84700525-84700544 MS.gene022830:intron 45.0%
! GGATGCGGTGTATTTTGCAA+TGG - chr8.1:84700446-84700465 MS.gene022830:intron 45.0%
! TATTTTGCAATGGAGCGGCT+TGG - chr8.1:84700456-84700475 MS.gene022830:intron 45.0%
! TGAGTAGAAAGGTACGAGGT+TGG + chr8.1:84700016-84700035 None:intergenic 45.0%
!!! TTTTACAATAATATTATTAT+TGG + chr8.1:84699520-84699539 None:intergenic 5.0%
ACTTCGGCGTCTTTCCAGAA+AGG + chr8.1:84700758-84700777 None:intergenic 50.0%
AGAATTGAACCTGGTCGAGC+CGG + chr8.1:84700627-84700646 None:intergenic 50.0%
CTCAAAGCCCTCGAAAACAC+TGG - chr8.1:84699907-84699926 MS.gene022830:CDS 50.0%
CTTGAAACCGGGTTTGGGTA+CGG - chr8.1:84700680-84700699 MS.gene022830:intron 50.0%
GCAGAAATCTCAGCACAACC+AGG - chr8.1:84699772-84699791 MS.gene022830:CDS 50.0%
GGGGAACCGAACATGTGAAT+TGG - chr8.1:84700893-84700912 MS.gene022830:intron 50.0%
GGTGGGACTTGCAACTTCAA+TGG - chr8.1:84701008-84701027 MS.gene022830:intron 50.0%
GTACAATGTGGGGCCATGTT+TGG + chr8.1:84700095-84700114 None:intergenic 50.0%
TGAGATTTCTGCACCTGTGG+TGG + chr8.1:84699764-84699783 None:intergenic 50.0%
TGCTGAGATTTCTGCACCTG+TGG + chr8.1:84699767-84699786 None:intergenic 50.0%
! GCTCAGTTTAGGAGTGTAGG+TGG - chr8.1:84700990-84701009 MS.gene022830:intron 50.0%
! GGTTCGAAACAGGGTTTTCC+GGG + chr8.1:84700924-84700943 None:intergenic 50.0%
! TGGGCTCAGTTTAGGAGTGT+AGG - chr8.1:84700987-84701006 MS.gene022830:intron 50.0%
!! CAGTGTTTTCGAGGGCTTTG+AGG + chr8.1:84699909-84699928 None:intergenic 50.0%
!! GAAACCGGTTGGCCTAATGA+TGG - chr8.1:84700507-84700526 MS.gene022830:intron 50.0%
!! TAATCATCTGGTGGCGGCAA+TGG + chr8.1:84700792-84700811 None:intergenic 50.0%
!! TCGAGGGCTTTGAGGATTTG+TGG + chr8.1:84699901-84699920 None:intergenic 50.0%
!! TGGGGAGCATGATGGATACT+TGG + chr8.1:84699936-84699955 None:intergenic 50.0%
AAAACCTCCGGTTGGGACAC+CGG - chr8.1:84700605-84700624 MS.gene022830:intron 55.0%
AGAGGGAGCAAACAAGACGG+CGG - chr8.1:84700839-84700858 MS.gene022830:intron 55.0%
AGCAAACAAGACGGCGGTAG+TGG - chr8.1:84700845-84700864 MS.gene022830:intron 55.0%
ATTTCTGCACCTGTGGTGGC+AGG + chr8.1:84699760-84699779 None:intergenic 55.0%
GGCAGAGGGAGCAAACAAGA+CGG - chr8.1:84700836-84700855 MS.gene022830:intron 55.0%
! AATGGAGCGGCTTGGGTATC+CGG - chr8.1:84700464-84700483 MS.gene022830:intron 55.0%
! TAAAGGGAAGGCGTGGTGTG+TGG - chr8.1:84700812-84700831 MS.gene022830:intron 55.0%
! TTGGGTACGGAGCGTCACTT+CGG - chr8.1:84700693-84700712 MS.gene022830:intron 55.0%
TGACGCTCCGTACCCAAACC+CGG + chr8.1:84700690-84700709 None:intergenic 60.0%
! TGGGTACGGAGCGTCACTTC+GGG - chr8.1:84700694-84700713 MS.gene022830:intron 60.0%
GCGTGGTGTGTGGTGGCAGA+GGG - chr8.1:84700822-84700841 MS.gene022830:intron 65.0%
! AGGGAAGGCGTGGTGTGTGG+TGG - chr8.1:84700815-84700834 MS.gene022830:intron 65.0%
GGCGTGGTGTGTGGTGGCAG+AGG - chr8.1:84700821-84700840 MS.gene022830:intron 70.0%
GGTCGAGCCGGTGTCCCAAC+CGG + chr8.1:84700615-84700634 None:intergenic 70.0%
!! GTTGGGACACCGGCTCGACC+AGG - chr8.1:84700615-84700634 MS.gene022830:intron 70.0%
CGAGCCGGTGTCCCAACCGG+AGG + chr8.1:84700612-84700631 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 84698939 84701288 84698939 ID=MS.gene022830
chr8.1 mRNA 84698939 84701288 84698939 ID=MS.gene022830.t1;Parent=MS.gene022830
chr8.1 exon 84701225 84701288 84701225 ID=MS.gene022830.t1.exon1;Parent=MS.gene022830.t1
chr8.1 CDS 84701225 84701288 84701225 ID=cds.MS.gene022830.t1;Parent=MS.gene022830.t1
chr8.1 exon 84699168 84700457 84699168 ID=MS.gene022830.t1.exon2;Parent=MS.gene022830.t1
chr8.1 CDS 84699168 84700457 84699168 ID=cds.MS.gene022830.t1;Parent=MS.gene022830.t1
chr8.1 exon 84698939 84698976 84698939 ID=MS.gene022830.t1.exon3;Parent=MS.gene022830.t1
chr8.1 CDS 84698939 84698976 84698939 ID=cds.MS.gene022830.t1;Parent=MS.gene022830.t1
Gene Sequence

>MS.gene022830

ATGAAAGCAATAGCTATGGTTGTGATTTTTCTATTTATCTTCTCTTCCTTATTCACTATATCAAGTAAGTCATTTCTCCTTCTCTATTTATCTTCTCCTTATTTACTACATCAATCAAAATCACAAACTTTGAGTTATTTTTATTTATTTATTTTCAACATGAATCAACTTTCATCTTTTAAAATGTAAAATTCTTGATAGCATCTCATCATGAGATAGCTAAGATGGTATATTTTCAATTTTTGAAATATTGGAGTTGAGCTTGATTGCATATTTGGAGACTAATTAATCCCACTACTATTTAATCTGTGTTTGTAAACCAACAATCTGAGCTTGATGGCACTTTAGTTTCACCAGTTTCACCCTAAAACCAAACATTCATTTAGATTTTAAGTTTCTAACGAAATAACAATATGGCTATTTCTATGTGTACAATTGAAGTAGGGGTTGTATCATACACAAGTTTATTTTCACTATTAAATGAATAAGTCTCATCATTGGATCAAGTGAGATTTTGTGGGATATATTGATTCAATGGTGGGAAAGAAAAAAAACTTGAAACATGGTAGAACATTATACCAATAATAATATTATTGTAAAACACTTAAAAATTCAACAACCTTTTATATGATTAACGTAGTTAAAACATATGAGTAATGAAGTGTTATATTTAGTTAGCAATTTATTAGTTATATACTCAACTTCATTAATCACAAACTTGAATGTGATTAACCACTATATATTAGAACATGATACATTCAAAAAAACATTTGTGATTATGATATAACTTAGTTTTTTCCTCTAACCCAATTTTCTAACCTGCCACCACAGGTGCAGAAATCTCAGCACAACCAGGAGTGAATTATGGACAACTAGGAAACAATCTTCCAACACCAACAACCTCAGTATCTCTCATCAAAAACCTCAAAGCCAAACGTGTCAAAATCTATGATGCAAATCCACAAATCCTCAAAGCCCTCGAAAACACTGGAATCCAAGTATCCATCATGCTCCCCAATGAACTTGTAACAAATGTATCCTCAAACCAAACACTTGCAAATCAATGGGTCCAAACCAACCTCGTACCTTTCTACTCAAAAACACTCATTCGTTATCTTCTTGTTGGTAATGAACTCATTTCCTCAACAACAAACCAAACATGGCCCCACATTGTACCTGCCATGTACAGAATGAAACACTCCTTAACTATTTTTGGTTTGCATAAAGTCAAAGTTGGAACACCTTTGGCAATGGATGTTTTACAAACATCATTTCCACCTTCAAATGGCACATTTAGAAATGACATTGCGTTGAGTGTTATGAAACCAATGTTAGAGTTTTTACATGTCACTAACTCTTTTTTCTTCTTAGATGTGTATCCATTTTTTGCTTGGACTTCAGATCCTATTAATATTAACTTGGATTATGCTCTTTTTGAGTCCGATAACATCACGGTTACAGATTCTGGTACGGGTTTGGTTTATACTAATTTGTTTGATCAAATGGTGGATGCGGTGTATTTTGCAATGGAGCGGCTTGGGTATCCGGATATTCAGATATTTATCGCTGAAACCGGTTGGCCTAATGATGGTGATTTGGACCAAGTTGGTGCGAATATTCACAATGCTGGTACATATAATAGGAACTTCGTGAAAAAAGTTACGAAAAAACCTCCGGTTGGGACACCGGCTCGACCAGGTTCAATTCTTCCGTCTTTTATATTTGCTTTGTATAATGAGAACTTGAAACCGGGTTTGGGTACGGAGCGTCACTTCGGGTTATTGTACCCAAATGGGTCGAGTATTTACGAGATTGACCTTTCTGGAAAGACGCCGAAGTCAGAGCTTAAGCCATTGCCGCCACCAGATGATTATAAAGGGAAGGCGTGGTGTGTGGTGGCAGAGGGAGCAAACAAGACGGCGGTAGTGGAAGCATTGTCATATGCTTGCTCACAGGGGAACCGAACATGTGAATTGGTTCAACCCGGAAAACCCTGTTTCGAACCTGATTCAGTTGTTGGTCATGCTAGCTATGCGTTTAGTTCTTACTGGGCTCAGTTTAGGAGTGTAGGTGGGACTTGCAACTTCAATGGTCTTGCCACTCAAATTGCAGAGGATCCAAGTAAGTTGTTTTTTTAATTGCTTTAAGAACATTTATTTTAGTTATACTTCCATAAAAACTGAATGATTATATAAAACACTAGAGAAGAAGAGGCCATAATCATGTTTTGATTTTAAAATCATTTTGTAATATTGATCATACCCTTTTGTTTTTATGTTTGACCGATACTTTGTACATTTTCATTTTTGAAGGTTATGGATCTTGCAAATACCCAAATGTGATTCTTTGA
Protein sequence

>MS.gene022830.t1

MKAIAMVVIFLFIFSSLFTISSAEISAQPGVNYGQLGNNLPTPTTSVSLIKNLKAKRVKIYDANPQILKALENTGIQVSIMLPNELVTNVSSNQTLANQWVQTNLVPFYSKTLIRYLLVGNELISSTTNQTWPHIVPAMYRMKHSLTIFGLHKVKVGTPLAMDVLQTSFPPSNGTFRNDIALSVMKPMLEFLHVTNSFFFLDVYPFFAWTSDPININLDYALFESDNITVTDSGTGLVYTNLFDQMVDAVYFAMERLGYPDIQIFIAETGWPNDGDLDQVGANIHNAGTYNRNFVKKVTKKPPVGTPARPGSILPSFIFALYNENLKPGLGTERHFGLLYPNGSSIYEIDLSGKTPKSELKPLPPPDDYKGKAWCVVAEGANKTAVVEALSYACSQGNRTCELVQPGKPCFEPDSVVGHASYAFSSYWAQFRSVGGTCNFNGLATQIAEDPSYGSCKYPNVIL