AlfalfaGEDB | MS.gene02320

M. sativa cultivar XinJiangDaYe / MS.gene02320

KEGG: -
GO: GO:0003674 GO:0004857 GO:0008150 GO:0008428 GO:0019219 GO:0019222 GO:0030234 GO:0031323 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0098772
PFAM: PF03737.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02320.t1	XP_013462438.1	97	166	5	0	1	166	1	166	4.10E-88	334

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02320.t1	Q9FH13	75.3	166	41	0	1	166	1	166	4.3e-70	265.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02320.t1	A0A072V558	97.0	166	5	0	1	166	1	166	3.0e-88	334.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene02320	MS.gene033474	PPI
MS.gene02320	MS.gene024038	PPI
MS.gene51161	MS.gene02320	PPI
MS.gene02320	MS.gene52579	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02320.t1	MTR_2g010815	96.988	166	5	1	166	1	166	2.20e-118	331
MS.gene02320.t1	MTR_2g010800	86.747	166	22	1	166	1	166	8.59e-105	297
MS.gene02320.t1	MTR_7g080040	65.060	166	58	1	166	1	166	1.59e-80	236

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02320.t1	AT5G56260	75.301	166	41	1	166	1	166	1.54e-90	261
MS.gene02320.t1	AT5G56260	75.301	166	41	1	166	1	166	1.54e-90	261
MS.gene02320.t1	AT5G56260	75.301	166	41	1	166	1	166	1.54e-90	261
MS.gene02320.t1	AT5G56260	75.301	166	41	1	166	33	198	1.03e-89	260
MS.gene02320.t1	AT5G56260	75.301	166	41	1	166	33	198	1.03e-89	260
MS.gene02320.t1	AT3G02770	65.060	166	58	1	166	1	166	9.27e-78	229
MS.gene02320.t1	AT5G16450	65.060	166	58	1	166	1	166	1.42e-77	228
MS.gene02320.t1	AT5G16450	65.060	166	58	1	166	1	166	1.42e-77	228

Find 39 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCTCTTGTTGGAGGGAATT+TGG	0.199853	2.1:+72827260	MS.gene02320:CDS
TGGCCAGTCTCGGGCATTCT+CGG	0.281731	2.1:+72827125	MS.gene02320:CDS
GCACAAAACATGGGGTGGTC+TGG	0.304278	2.1:+72827291	MS.gene02320:CDS
GGCCAGTCTCGGGCATTCTC+GGG	0.351904	2.1:+72827126	MS.gene02320:CDS
AAAAGGAGAAGGAAGGGTTT+TGG	0.369523	2.1:+72827209	MS.gene02320:CDS
TGGTCTGGGATTGTTGTGAA+TGG	0.381293	2.1:+72827306	MS.gene02320:CDS
CACAAAACATGGGGTGGTCT+GGG	0.391877	2.1:+72827292	MS.gene02320:CDS
CCCGAGACTGGCCATACATC+TGG	0.415145	2.1:-72827116	None:intergenic
GCCCCGAGAATGCCCGAGAC+TGG	0.420007	2.1:-72827128	None:intergenic
TCAAGTTGGTGTTAGAGCTT+TGG	0.422544	2.1:+72827362	MS.gene02320:CDS
AACGCAACACATTTAACAAA+TGG	0.449158	2.1:+72827066	MS.gene02320:CDS
TCCAGATGTATGGCCAGTCT+CGG	0.458278	2.1:+72827115	MS.gene02320:CDS
GGTGCAGTTGGCACAAAACA+TGG	0.464361	2.1:+72827281	MS.gene02320:CDS
CATCCAGTGTTCCAGATGTA+TGG	0.472302	2.1:+72827105	MS.gene02320:CDS
CAGTCTAGTACTATTTCAAA+TGG	0.479945	2.1:-72827523	None:intergenic
TCAAATGGACAACTCAAACT+TGG	0.482072	2.1:-72827508	None:intergenic
TGGAGGGAATTTGGTGCAGT+TGG	0.484334	2.1:+72827269	MS.gene02320:CDS
AGAGAACTTCTTGAAACAAA+AGG	0.491488	2.1:+72827192	MS.gene02320:CDS
GAGATTAATTTGTGTCAAGT+TGG	0.491931	2.1:+72827348	MS.gene02320:CDS
CATGTTGCTGTTTATGTTGG+AGG	0.502059	2.1:+72827429	MS.gene02320:CDS
TTGAAACAAAAGGAGAAGGA+AGG	0.507552	2.1:+72827202	MS.gene02320:CDS
AAACATGTTGCTGTTTATGT+TGG	0.508506	2.1:+72827426	MS.gene02320:CDS
TGGCCATACATCTGGAACAC+TGG	0.512769	2.1:-72827108	None:intergenic
CCAGATGTATGGCCAGTCTC+GGG	0.532810	2.1:+72827116	MS.gene02320:CDS
GTGTCACATATCTCTGCTGT+TGG	0.545822	2.1:-72827042	None:intergenic
TGAAACAAAAGGAGAAGGAA+GGG	0.548341	2.1:+72827203	MS.gene02320:CDS
GCCAGTCTCGGGCATTCTCG+GGG	0.553955	2.1:+72827127	MS.gene02320:CDS
AGCATGAGGTGTGCTCTTGT+TGG	0.557997	2.1:+72827249	MS.gene02320:CDS
ATGAGGTGTGCTCTTGTTGG+AGG	0.569564	2.1:+72827252	MS.gene02320:CDS
CTTCTTGAAACAAAAGGAGA+AGG	0.585218	2.1:+72827198	MS.gene02320:CDS
TGGCTCTATGCAGATAATGA+TGG	0.590987	2.1:+72827474	MS.gene02320:CDS
ATTTAACAAATGGTGACTTG+AGG	0.616446	2.1:+72827076	MS.gene02320:CDS
AGTGTTTGAAGACAATGTGT+TGG	0.617013	2.1:+72827167	MS.gene02320:CDS
GTGCAGTTGGCACAAAACAT+GGG	0.623381	2.1:+72827282	MS.gene02320:CDS
GAATGGTTGTATTAGAGATG+TGG	0.623790	2.1:+72827323	MS.gene02320:CDS
TGAGGTGTGCTCTTGTTGGA+GGG	0.651891	2.1:+72827253	MS.gene02320:CDS
AGTTGGCACAAAACATGGGG+TGG	0.672135	2.1:+72827286	MS.gene02320:CDS
TGCAGTTGGCACAAAACATG+GGG	0.672279	2.1:+72827283	MS.gene02320:CDS
TTGATGGAGGAGGAAGCATG+AGG	0.673507	2.1:+72827235	MS.gene02320:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CCACTTAAATCCAATAAAAA+CGG	+	chr2.1:72827393-72827412	MS.gene02320:CDS	25.0%
!!	ATTGTCTTCAAACACTTTTA+CGG	-	chr2.1:72827163-72827182	None:intergenic	25.0%
	AAATCCAATAAAAACGGTAC+TGG	+	chr2.1:72827399-72827418	MS.gene02320:CDS	30.0%
	AACGCAACACATTTAACAAA+TGG	+	chr2.1:72827066-72827085	MS.gene02320:CDS	30.0%
	AGAGAACTTCTTGAAACAAA+AGG	+	chr2.1:72827192-72827211	MS.gene02320:CDS	30.0%
	ATTTAACAAATGGTGACTTG+AGG	+	chr2.1:72827076-72827095	MS.gene02320:CDS	30.0%
!!	AAACATGTTGCTGTTTATGT+TGG	+	chr2.1:72827426-72827445	MS.gene02320:CDS	30.0%
!!	CCGTTTTTATTGGATTTAAG+TGG	-	chr2.1:72827396-72827415	None:intergenic	30.0%
!!	GAGATTAATTTGTGTCAAGT+TGG	+	chr2.1:72827348-72827367	MS.gene02320:CDS	30.0%
	AGTGTTTGAAGACAATGTGT+TGG	+	chr2.1:72827167-72827186	MS.gene02320:CDS	35.0%
	CTTCTTGAAACAAAAGGAGA+AGG	+	chr2.1:72827198-72827217	MS.gene02320:CDS	35.0%
	GAATGGTTGTATTAGAGATG+TGG	+	chr2.1:72827323-72827342	MS.gene02320:CDS	35.0%
	TCAAATGGACAACTCAAACT+TGG	-	chr2.1:72827511-72827530	None:intergenic	35.0%
	TGAAACAAAAGGAGAAGGAA+GGG	+	chr2.1:72827203-72827222	MS.gene02320:CDS	35.0%
	TTGAAACAAAAGGAGAAGGA+AGG	+	chr2.1:72827202-72827221	MS.gene02320:CDS	35.0%
!	AACACTTTTACGGTAACAAC+AGG	-	chr2.1:72827153-72827172	None:intergenic	35.0%
!!	CTTTTATTCGTGATGGAGAA+TGG	+	chr2.1:72827454-72827473	MS.gene02320:CDS	35.0%
!!	TTCACCAGTACCGTTTTTAT+TGG	-	chr2.1:72827406-72827425	None:intergenic	35.0%
!	AAAAGGAGAAGGAAGGGTTT+TGG	+	chr2.1:72827209-72827228	MS.gene02320:CDS	40.0%
!	GGAGGAACTTTTATTCGTGA+TGG	+	chr2.1:72827447-72827466	MS.gene02320:CDS	40.0%
!	TGGCTCTATGCAGATAATGA+TGG	+	chr2.1:72827474-72827493	MS.gene02320:CDS	40.0%
!!	CATGTTGCTGTTTATGTTGG+AGG	+	chr2.1:72827429-72827448	MS.gene02320:CDS	40.0%
!!	TCAAGTTGGTGTTAGAGCTT+TGG	+	chr2.1:72827362-72827381	MS.gene02320:CDS	40.0%
!!!	AGGGTTTTGGTTATTGATGG+AGG	+	chr2.1:72827222-72827241	MS.gene02320:CDS	40.0%
!!!	GGAAGGGTTTTGGTTATTGA+TGG	+	chr2.1:72827219-72827238	MS.gene02320:CDS	40.0%
!!!	GTTTTGGTTATTGATGGAGG+AGG	+	chr2.1:72827225-72827244	MS.gene02320:CDS	40.0%
	CATCCAGTGTTCCAGATGTA+TGG	+	chr2.1:72827105-72827124	MS.gene02320:CDS	45.0%
	GTGCAGTTGGCACAAAACAT+GGG	+	chr2.1:72827282-72827301	MS.gene02320:CDS	45.0%
	GTGTCACATATCTCTGCTGT+TGG	-	chr2.1:72827045-72827064	None:intergenic	45.0%
	TGCAGTTGGCACAAAACATG+GGG	+	chr2.1:72827283-72827302	MS.gene02320:CDS	45.0%
	TGCTCTTGTTGGAGGGAATT+TGG	+	chr2.1:72827260-72827279	MS.gene02320:CDS	45.0%
!	TGGTCTGGGATTGTTGTGAA+TGG	+	chr2.1:72827306-72827325	MS.gene02320:CDS	45.0%
	AGCATGAGGTGTGCTCTTGT+TGG	+	chr2.1:72827249-72827268	MS.gene02320:CDS	50.0%
	AGTTGGCACAAAACATGGGG+TGG	+	chr2.1:72827286-72827305	MS.gene02320:CDS	50.0%
	ATGAGGTGTGCTCTTGTTGG+AGG	+	chr2.1:72827252-72827271	MS.gene02320:CDS	50.0%
	CACAAAACATGGGGTGGTCT+GGG	+	chr2.1:72827292-72827311	MS.gene02320:CDS	50.0%
	GGTGCAGTTGGCACAAAACA+TGG	+	chr2.1:72827281-72827300	MS.gene02320:CDS	50.0%
	TCCAGATGTATGGCCAGTCT+CGG	+	chr2.1:72827115-72827134	MS.gene02320:CDS	50.0%
	TGAGGTGTGCTCTTGTTGGA+GGG	+	chr2.1:72827253-72827272	MS.gene02320:CDS	50.0%
!	TGGCCATACATCTGGAACAC+TGG	-	chr2.1:72827111-72827130	None:intergenic	50.0%
!	TTGATGGAGGAGGAAGCATG+AGG	+	chr2.1:72827235-72827254	MS.gene02320:CDS	50.0%
!!	TGGAGGGAATTTGGTGCAGT+TGG	+	chr2.1:72827269-72827288	MS.gene02320:CDS	50.0%
	CCAGATGTATGGCCAGTCTC+GGG	+	chr2.1:72827116-72827135	MS.gene02320:CDS	55.0%
	GCACAAAACATGGGGTGGTC+TGG	+	chr2.1:72827291-72827310	MS.gene02320:CDS	55.0%
	TGGCCAGTCTCGGGCATTCT+CGG	+	chr2.1:72827125-72827144	MS.gene02320:CDS	60.0%
!	CCCGAGACTGGCCATACATC+TGG	-	chr2.1:72827119-72827138	None:intergenic	60.0%
	GCCAGTCTCGGGCATTCTCG+GGG	+	chr2.1:72827127-72827146	MS.gene02320:CDS	65.0%
	GGCCAGTCTCGGGCATTCTC+GGG	+	chr2.1:72827126-72827145	MS.gene02320:CDS	65.0%
	GCCCCGAGAATGCCCGAGAC+TGG	-	chr2.1:72827131-72827150	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	72827030	72827530	72827030	ID=MS.gene02320
chr2.1	mRNA	72827030	72827530	72827030	ID=MS.gene02320.t1;Parent=MS.gene02320
chr2.1	exon	72827030	72827530	72827030	ID=MS.gene02320.t1.exon1;Parent=MS.gene02320.t1
chr2.1	CDS	72827030	72827530	72827030	ID=cds.MS.gene02320.t1;Parent=MS.gene02320.t1

Gene Sequence

>MS.gene02320

ATGGATTCTCTACCAACAGCAGAGATATGTGACACAAACGCAACACATTTAACAAATGGTGACTTGAGGATACTTCATCCAGTGTTCCAGATGTATGGCCAGTCTCGGGCATTCTCGGGGCCTGTTGTTACCGTAAAAGTGTTTGAAGACAATGTGTTGGTAAGAGAACTTCTTGAAACAAAAGGAGAAGGAAGGGTTTTGGTTATTGATGGAGGAGGAAGCATGAGGTGTGCTCTTGTTGGAGGGAATTTGGTGCAGTTGGCACAAAACATGGGGTGGTCTGGGATTGTTGTGAATGGTTGTATTAGAGATGTGGATGAGATTAATTTGTGTCAAGTTGGTGTTAGAGCTTTGGCTTCTCATCCACTTAAATCCAATAAAAACGGTACTGGTGAAAAACATGTTGCTGTTTATGTTGGAGGAACTTTTATTCGTGATGGAGAATGGCTCTATGCAGATAATGATGGTATCATTGTATCCAAGTTTGAGTTGTCCATTTGA

Protein sequence

>MS.gene02320.t1

MDSLPTAEICDTNATHLTNGDLRILHPVFQMYGQSRAFSGPVVTVKVFEDNVLVRELLETKGEGRVLVIDGGGSMRCALVGGNLVQLAQNMGWSGIVVNGCIRDVDEINLCQVGVRALASHPLKSNKNGTGEKHVAVYVGGTFIRDGEWLYADNDGIIVSKFELSI