AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene023615

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023615.t1 MTR_8g087540 97.257 401 11 0 1 401 1 401 0.0 814
MS.gene023615.t1 MTR_2g017890 40.633 411 186 9 1 383 2 382 6.88e-87 269
MS.gene023615.t1 MTR_6g043060 36.212 359 177 9 6 358 16 328 3.69e-63 206
MS.gene023615.t1 MTR_5g029680 51.630 184 82 1 11 187 31 214 1.13e-60 204
MS.gene023615.t1 MTR_8g083100 52.239 201 84 4 13 207 23 217 1.94e-60 203
MS.gene023615.t1 MTR_2g100680 49.751 201 92 2 12 207 31 227 3.18e-60 202
MS.gene023615.t1 MTR_2g100670 49.744 195 86 2 1 187 14 204 4.41e-54 182
MS.gene023615.t1 MTR_2g100670 49.744 195 86 2 1 187 122 312 5.61e-53 182
MS.gene023615.t1 MTR_1g102860 36.082 291 166 4 11 288 39 322 1.10e-52 179
MS.gene023615.t1 MTR_4g077970 44.865 185 94 2 10 187 10 193 1.50e-51 180
MS.gene023615.t1 MTR_7g095680 39.130 230 126 3 11 226 48 277 9.09e-49 169
MS.gene023615.t1 MTR_3g104550 41.584 202 114 1 11 208 45 246 1.09e-46 164
MS.gene023615.t1 MTR_1g061170 41.935 186 106 1 8 193 45 228 3.55e-46 165
MS.gene023615.t1 MTR_8g105780 46.875 192 91 3 12 194 22 211 1.12e-45 161
MS.gene023615.t1 MTR_1g071360 42.541 181 87 3 12 189 32 198 2.17e-43 154
MS.gene023615.t1 MTR_1g071360 42.541 181 87 3 12 189 29 195 2.86e-43 155
MS.gene023615.t1 MTR_2g043940 46.995 183 80 4 52 229 11 181 1.75e-41 150
MS.gene023615.t1 MTR_6g086805 38.308 201 116 2 10 206 9 205 4.38e-41 148
MS.gene023615.t1 MTR_5g010680 64.356 101 35 1 4 103 15 115 8.21e-41 148
MS.gene023615.t1 MTR_3g101870 64.646 99 34 1 6 103 17 115 2.77e-39 144
MS.gene023615.t1 MTR_5g089170 66.667 99 32 1 6 103 18 116 8.50e-39 140
MS.gene023615.t1 MTR_5g017470 58.824 102 42 0 8 109 5 106 1.00e-38 140
MS.gene023615.t1 MTR_1g100777 58.163 98 41 0 8 105 14 111 3.63e-37 137
MS.gene023615.t1 MTR_0020s0020 59.596 99 39 1 6 103 26 124 7.82e-36 134
MS.gene023615.t1 MTR_7g091370 49.123 114 58 0 3 116 13 126 2.74e-34 127
MS.gene023615.t1 MTR_4g088900 37.714 175 91 5 12 176 33 199 7.11e-30 115
MS.gene023615.t1 MTR_5g047190 53.061 98 39 2 20 110 1 98 1.86e-27 105
MS.gene023615.t1 MTR_4g045757 63.333 60 22 0 10 69 12 71 1.29e-21 88.6
MS.gene023615.t1 MTR_5g022830 87.805 41 5 0 361 401 40 80 5.13e-19 81.3
MS.gene023615.t1 MTR_6g044340 28.571 308 148 15 64 358 14 262 2.40e-17 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene023615.t1 AT4G18880 51.095 411 179 11 1 400 1 400 3.98e-115 342
MS.gene023615.t1 AT5G45710 49.492 394 145 8 1 391 1 343 2.30e-110 328
MS.gene023615.t1 AT5G45710 49.492 394 145 8 1 391 1 343 2.30e-110 328
MS.gene023615.t1 AT4G13980 48.500 200 94 2 13 207 24 219 2.28e-59 200
MS.gene023615.t1 AT1G32330 41.949 236 118 2 6 222 31 266 1.24e-58 198
MS.gene023615.t1 AT1G32330 41.949 236 118 2 6 222 31 266 1.53e-58 198
MS.gene023615.t1 AT5G45710 40.940 298 123 8 96 391 1 247 1.77e-55 183
MS.gene023615.t1 AT5G45710 40.940 298 123 8 96 391 1 247 1.77e-55 183
MS.gene023615.t1 AT5G16820 42.991 214 109 2 8 208 23 236 1.45e-54 187
MS.gene023615.t1 AT5G16820 42.991 214 109 2 8 208 23 236 1.45e-54 187
MS.gene023615.t1 AT4G17750 45.026 191 91 1 11 187 51 241 7.66e-54 186
MS.gene023615.t1 AT3G22830 41.256 223 117 2 8 216 57 279 1.92e-53 183
MS.gene023615.t1 AT3G02990 47.283 184 93 1 8 187 19 202 3.84e-53 184
MS.gene023615.t1 AT2G26150 41.262 206 113 1 11 208 43 248 2.28e-50 173
MS.gene023615.t1 AT2G26150 41.262 206 113 1 11 208 43 248 2.28e-50 173
MS.gene023615.t1 AT5G03720 45.810 179 89 2 10 185 53 226 7.43e-49 171
MS.gene023615.t1 AT5G03720 45.810 179 89 2 10 185 53 226 7.43e-49 171
MS.gene023615.t1 AT5G43840 43.000 200 90 3 11 208 16 193 1.36e-46 161
MS.gene023615.t1 AT1G67970 43.386 189 100 3 10 194 17 202 1.71e-45 161
MS.gene023615.t1 AT3G51910 45.652 184 85 3 11 194 28 196 4.30e-44 154
MS.gene023615.t1 AT5G54070 39.698 199 110 2 10 208 69 257 3.41e-41 148
MS.gene023615.t1 AT3G24520 39.247 186 86 4 10 195 15 173 1.69e-40 147
MS.gene023615.t1 AT1G46264 63.000 100 36 1 5 103 26 125 1.78e-39 144
MS.gene023615.t1 AT3G63350 38.725 204 111 3 1 204 21 210 1.90e-39 142
MS.gene023615.t1 AT5G62020 51.181 127 57 2 3 128 13 135 2.08e-38 140
MS.gene023615.t1 AT4G36990 63.265 98 35 1 9 105 10 107 9.05e-38 138
MS.gene023615.t1 AT4G11660 59.596 99 39 1 6 103 52 150 1.64e-36 137
MS.gene023615.t1 AT2G41690 58.491 106 43 1 11 115 39 144 2.19e-36 133
MS.gene023615.t1 AT4G18870 54.639 97 39 2 5 99 6 99 6.27e-27 109
MS.gene023615.t1 AT4G18870 52.174 92 44 0 8 99 142 233 2.05e-26 107
MS.gene023615.t1 AT1G77570 43.333 90 48 2 13 99 27 116 1.45e-17 79.3
MS.gene023615.t1 AT2G26150 33.621 116 77 0 72 187 49 164 1.14e-16 80.1
MS.gene023615.t1 AT2G26150 33.621 116 77 0 72 187 49 164 1.14e-16 80.1
MS.gene023615.t1 AT4G19630 38.947 95 55 1 8 99 7 101 2.15e-15 72.8

Find 85 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGGCATCCGTGGCATTT+TGG 0.204494 4.4:+13252587 MS.gene023615:CDS
TCAACGTTCATGTCAATTTC+AGG 0.245219 4.4:-13252745 None:intergenic
ATATGTATGTGCAGGGATTT+AGG 0.272285 4.4:+13252038 MS.gene023615:intron
AGCAAAACCCTTGCGAATTC+CGG 0.278319 4.4:-13251852 None:intergenic
CACTCATTCAAACATGGATT+TGG 0.293073 4.4:+13252648 MS.gene023615:CDS
CTGGGGTTAATGATGTATTC+TGG 0.294292 4.4:+13252836 MS.gene023615:CDS
CTAACATGCCCCATCTGTTC+TGG 0.312124 4.4:-13253003 None:intergenic
CGAAGAGATACCAGCATGAT+TGG 0.339945 4.4:+13252272 MS.gene023615:CDS
CTAGTGATCATGGTAGATTT+TGG 0.344391 4.4:+13252953 MS.gene023615:CDS
AGAATCACCACTGCAGCAAC+TGG 0.356379 4.4:+13252817 MS.gene023615:CDS
AAGAGATTTACAGCAATCAT+AGG 0.368528 4.4:-13252397 None:intergenic
GACGTTAAACACCGAGAGAT+TGG 0.369357 4.4:+13252558 MS.gene023615:CDS
TGCCTTTCAGACTCCGTTAG+AGG 0.391700 4.4:-13252190 None:intergenic
GGAGAGAAAACGAAGGTTGC+CGG 0.399062 4.4:+13252438 MS.gene023615:CDS
ATTCAGCTGCCTGATAAAAC+TGG 0.407135 4.4:-13251904 None:intergenic
CTCTCCATGGCTTCTGCCAG+TGG 0.418903 4.4:-13252421 None:intergenic
TCATAAGTCTTTGCCAGAAA+AGG 0.419332 4.4:-13251753 None:intergenic
GCCTCAAGAGCAGCAGCAGC+TGG 0.427478 4.4:-13252769 None:intergenic
TCTGGCAAAGACTTATGAAA+TGG 0.438206 4.4:+13251758 MS.gene023615:CDS
TGAAATAGAGAAGCTGAAAC+AGG 0.448407 4.4:+13252225 MS.gene023615:CDS
GATGCACTCCACGAAACAAT+AGG 0.454683 4.4:-13251801 None:intergenic
TGGGGTTAATGATGTATTCT+GGG 0.456218 4.4:+13252837 MS.gene023615:CDS
ACCAGCTGCTGCTGCTCTTG+AGG 0.466489 4.4:+13252768 MS.gene023615:CDS
GCCTTTCAGACTCCGTTAGA+GGG 0.473627 4.4:-13252189 None:intergenic
ATGTCAATTTCAGGTGACTT+AGG 0.477806 4.4:-13252736 None:intergenic
TGCAGAGAATGACTATGAAC+GGG 0.482637 4.4:-13252148 None:intergenic
TCCTGGTGCATCGGAAGCAC+AGG 0.486616 4.4:+13252879 MS.gene023615:CDS
GAGACTCTTGCACATGAAGT+TGG 0.497289 4.4:+13252610 MS.gene023615:CDS
CGTTCACACTCATTCAAACA+TGG 0.502442 4.4:+13252642 MS.gene023615:CDS
GAAGGAAAACCTAGTGATCA+TGG 0.502730 4.4:+13252943 MS.gene023615:CDS
TTGGCAAATTCCCACTGCTC+CGG 0.502904 4.4:-13252070 None:intergenic
GGGATTTAGGAAAGTTGATC+CGG 0.507404 4.4:+13252051 MS.gene023615:intron
CTTGCCACTGGCAGAAGCCA+TGG 0.509175 4.4:+13252417 MS.gene023615:CDS
TTTCGTTGTGTGGAATCCTC+CGG 0.511600 4.4:+13251833 MS.gene023615:CDS
TCCTGTGCTTCCGATGCACC+AGG 0.511617 4.4:-13252880 None:intergenic
GAAGAGATACCAGCATGATT+GGG 0.512133 4.4:+13252273 MS.gene023615:CDS
TGCTGTAAATCTCTTGCCAC+TGG 0.515181 4.4:+13252405 MS.gene023615:CDS
GGAAAGTTGATCCGGAGCAG+TGG 0.515618 4.4:+13252059 MS.gene023615:CDS
CAGTTCTTGACAGAGGATCC+TGG 0.516203 4.4:+13252862 MS.gene023615:CDS
AGACTTCTGTAGCTTTGCCC+AGG 0.516910 4.4:+13252509 MS.gene023615:CDS
AAACAATCATCCAGAACAGA+TGG 0.518388 4.4:+13252993 MS.gene023615:CDS
CCTCGGATCCTATTGTTTCG+TGG 0.527360 4.4:+13251793 MS.gene023615:CDS
TTATTGTGCTTAAAGAATCT+CGG 0.532647 4.4:-13251876 None:intergenic
CGAACAAAAGTTTCGTTGTG+TGG 0.533195 4.4:+13251823 MS.gene023615:CDS
GCATTGTTCGAAAGATCAGT+TGG 0.538254 4.4:+13252315 MS.gene023615:CDS
GTCAAAGTAGAGTTATCCTC+TGG 0.546841 4.4:-13252538 None:intergenic
AGCTAGTGTTGAAGATGCTA+TGG 0.547413 4.4:+13252486 MS.gene023615:CDS
CTCAAGTTGATCCAATCTCT+CGG 0.553126 4.4:-13252569 None:intergenic
GACAGAGGATCCTGGTGCAT+CGG 0.553354 4.4:+13252870 MS.gene023615:CDS
ATTAACCCCAGTTGCTGCAG+TGG 0.553513 4.4:-13252824 None:intergenic
CCCCTCTAACGGAGTCTGAA+AGG 0.554429 4.4:+13252188 MS.gene023615:CDS
ATGCAGAGAATGACTATGAA+CGG 0.560960 4.4:-13252149 None:intergenic
GCTGCAGTGGTGATTCTCGC+AGG 0.569378 4.4:-13252811 None:intergenic
TAAGTCTTTGCCAGAAAAGG+AGG 0.570699 4.4:-13251750 None:intergenic
CAAAATCTACCATGATCACT+AGG 0.571873 4.4:-13252952 None:intergenic
AACAATCATCCAGAACAGAT+GGG 0.574845 4.4:+13252994 MS.gene023615:CDS
GGAATCCTCCGGAATTCGCA+AGG 0.575730 4.4:+13251844 MS.gene023615:CDS
TTAAAGCAACCACTTCTCGC+CGG 0.577237 4.4:-13252457 None:intergenic
GAAAGTTGATCCGGAGCAGT+GGG 0.578322 4.4:+13252060 MS.gene023615:CDS
CATGCCTTTCCCAATCATGC+TGG 0.579073 4.4:-13252282 None:intergenic
GAATCACCACTGCAGCAACT+GGG 0.580374 4.4:+13252818 MS.gene023615:CDS
CGAAGGTTGCCGGCGAGAAG+TGG 0.586677 4.4:+13252448 MS.gene023615:CDS
CCTTTCAGACTCCGTTAGAG+GGG 0.598591 4.4:-13252188 None:intergenic
TGAATATTCTTCATCAGATG+CGG 0.602613 4.4:-13252115 None:intergenic
GACTTCTGTAGCTTTGCCCA+GGG 0.603526 4.4:+13252510 MS.gene023615:CDS
GATACCAGCATGATTGGGAA+AGG 0.606022 4.4:+13252278 MS.gene023615:CDS
AAAACCCTTGCGAATTCCGG+AGG 0.607238 4.4:-13251849 None:intergenic
TCAAGCACAAGCCCCTCTAA+CGG 0.613167 4.4:+13252177 MS.gene023615:CDS
GGCAAAGACTTATGAAATGG+TGG 0.613237 4.4:+13251761 MS.gene023615:CDS
GGATCAACTTGAGGCATCCG+TGG 0.615719 4.4:+13252579 MS.gene023615:CDS
CGAGAGATTGGATCAACTTG+AGG 0.627773 4.4:+13252570 MS.gene023615:CDS
AATGGTGGATGATCGATCCT+CGG 0.628991 4.4:+13251776 MS.gene023615:CDS
GAATCCTCCGGAATTCGCAA+GGG 0.629052 4.4:+13251845 MS.gene023615:CDS
TGATGAAGAATATTCATAGA+CGG 0.629516 4.4:+13252122 MS.gene023615:CDS
CTGCAATGCTTCAGAAACAG+AGG 0.634333 4.4:-13252371 None:intergenic
ATATCTTGTCAGCAACTAGA+CGG 0.636770 4.4:+13252703 MS.gene023615:CDS
ACAATCATCCAGAACAGATG+GGG 0.647372 4.4:+13252995 MS.gene023615:CDS
CTGGGAACAGTTCTTGACAG+AGG 0.655854 4.4:+13252855 MS.gene023615:CDS
AAGAGTCTCCCAAAATGCCA+CGG 0.657362 4.4:-13252596 None:intergenic
AATCACCACTGCAGCAACTG+GGG 0.658502 4.4:+13252819 MS.gene023615:CDS
GGATAGAGAACAACTCCTCG+TGG 0.661247 4.4:+13252246 MS.gene023615:CDS
CCACGAAACAATAGGATCCG+AGG 0.666703 4.4:-13251793 None:intergenic
GGTATCTCTTCGTCTCCACG+AGG 0.715796 4.4:-13252261 None:intergenic
TTTGCCCAGGGAAAATCCAG+AGG 0.715905 4.4:+13252522 MS.gene023615:CDS
AAGCCATGGAGAGAAAACGA+AGG 0.761326 4.4:+13252431 MS.gene023615:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTATTTACTGATTC+TGG + chr4.4:13251987-13252006 MS.gene023615:intron 15.0%
! AATAGTAGCAGAAAAAATGA+AGG + chr4.4:13252925-13252944 MS.gene023615:CDS 25.0%
! TATGTAAGCAAATTTGATGT+CGG + chr4.4:13251930-13251949 MS.gene023615:intron 25.0%
! TGATGAAGAATATTCATAGA+CGG + chr4.4:13252122-13252141 MS.gene023615:CDS 25.0%
! TTATTGTGCTTAAAGAATCT+CGG - chr4.4:13251879-13251898 None:intergenic 25.0%
!! TGCCAATGATGATTTTATAA+GGG + chr4.4:13252087-13252106 MS.gene023615:CDS 25.0%
!! TTGCCAATGATGATTTTATA+AGG + chr4.4:13252086-13252105 MS.gene023615:CDS 25.0%
!!! TATTTACTGATTCTGGTTTT+TGG + chr4.4:13251994-13252013 MS.gene023615:intron 25.0%
AAGAGATTTACAGCAATCAT+AGG - chr4.4:13252400-13252419 None:intergenic 30.0%
GAAAGATCAGTTGGAAAAAT+TGG + chr4.4:13252324-13252343 MS.gene023615:CDS 30.0%
TCATAGTATATGTATGTGCA+GGG + chr4.4:13252031-13252050 MS.gene023615:intron 30.0%
TGAATATTCTTCATCAGATG+CGG - chr4.4:13252118-13252137 None:intergenic 30.0%
TTCATAGTATATGTATGTGC+AGG + chr4.4:13252030-13252049 MS.gene023615:intron 30.0%
! CCAATGATGATTTTATAAGG+GGG + chr4.4:13252089-13252108 MS.gene023615:CDS 30.0%
! GCCAATGATGATTTTATAAG+GGG + chr4.4:13252088-13252107 MS.gene023615:CDS 30.0%
!! ATAACTTCTCCAGTTTTATC+AGG + chr4.4:13251895-13251914 MS.gene023615:CDS 30.0%
AAACAATCATCCAGAACAGA+TGG + chr4.4:13252993-13253012 MS.gene023615:CDS 35.0%
AACAATCATCCAGAACAGAT+GGG + chr4.4:13252994-13253013 MS.gene023615:CDS 35.0%
ATATCTTGTCAGCAACTAGA+CGG + chr4.4:13252703-13252722 MS.gene023615:CDS 35.0%
ATATGTATGTGCAGGGATTT+AGG + chr4.4:13252038-13252057 MS.gene023615:intron 35.0%
ATGCAGAGAATGACTATGAA+CGG - chr4.4:13252152-13252171 None:intergenic 35.0%
CAAAATCTACCATGATCACT+AGG - chr4.4:13252955-13252974 None:intergenic 35.0%
CACTCATTCAAACATGGATT+TGG + chr4.4:13252648-13252667 MS.gene023615:CDS 35.0%
CCCCCTTATAAAATCATCAT+TGG - chr4.4:13252092-13252111 None:intergenic 35.0%
TCAACGTTCATGTCAATTTC+AGG - chr4.4:13252748-13252767 None:intergenic 35.0%
TCATAAGTCTTTGCCAGAAA+AGG - chr4.4:13251756-13251775 None:intergenic 35.0%
TCTGGCAAAGACTTATGAAA+TGG + chr4.4:13251758-13251777 MS.gene023615:CDS 35.0%
TGAAATAGAGAAGCTGAAAC+AGG + chr4.4:13252225-13252244 MS.gene023615:CDS 35.0%
TGGGGTTAATGATGTATTCT+GGG + chr4.4:13252837-13252856 MS.gene023615:CDS 35.0%
! ATGTCAATTTCAGGTGACTT+AGG - chr4.4:13252739-13252758 None:intergenic 35.0%
! CTAGTGATCATGGTAGATTT+TGG + chr4.4:13252953-13252972 MS.gene023615:CDS 35.0%
ACAATCATCCAGAACAGATG+GGG + chr4.4:13252995-13253014 MS.gene023615:CDS 40.0%
ATTCAGCTGCCTGATAAAAC+TGG - chr4.4:13251907-13251926 None:intergenic 40.0%
CGAACAAAAGTTTCGTTGTG+TGG + chr4.4:13251823-13251842 MS.gene023615:CDS 40.0%
CGTTCACACTCATTCAAACA+TGG + chr4.4:13252642-13252661 MS.gene023615:CDS 40.0%
CTGGGGTTAATGATGTATTC+TGG + chr4.4:13252836-13252855 MS.gene023615:CDS 40.0%
GAAGAGATACCAGCATGATT+GGG + chr4.4:13252273-13252292 MS.gene023615:CDS 40.0%
GAAGGAAAACCTAGTGATCA+TGG + chr4.4:13252943-13252962 MS.gene023615:CDS 40.0%
GCATTGTTCGAAAGATCAGT+TGG + chr4.4:13252315-13252334 MS.gene023615:CDS 40.0%
GGCAAAGACTTATGAAATGG+TGG + chr4.4:13251761-13251780 MS.gene023615:CDS 40.0%
GTCAAAGTAGAGTTATCCTC+TGG - chr4.4:13252541-13252560 None:intergenic 40.0%
TAAGTCTTTGCCAGAAAAGG+AGG - chr4.4:13251753-13251772 None:intergenic 40.0%
TGCAGAGAATGACTATGAAC+GGG - chr4.4:13252151-13252170 None:intergenic 40.0%
! AGCTAGTGTTGAAGATGCTA+TGG + chr4.4:13252486-13252505 MS.gene023615:CDS 40.0%
! GGGATTTAGGAAAGTTGATC+CGG + chr4.4:13252051-13252070 MS.gene023615:intron 40.0%
! GTGATCATGGTAGATTTTGG+TGG + chr4.4:13252956-13252975 MS.gene023615:CDS 40.0%
! TTATCCTCTGGATTTTCCCT+GGG - chr4.4:13252529-13252548 None:intergenic 40.0%
!! CTCAAGTTGATCCAATCTCT+CGG - chr4.4:13252572-13252591 None:intergenic 40.0%
!! GTAGATTTTGGTGGAACATG+CGG + chr4.4:13252965-13252984 MS.gene023615:CDS 40.0%
AAGAGTCTCCCAAAATGCCA+CGG - chr4.4:13252599-13252618 None:intergenic 45.0%
AAGCCATGGAGAGAAAACGA+AGG + chr4.4:13252431-13252450 MS.gene023615:CDS 45.0%
AGCAAAACCCTTGCGAATTC+CGG - chr4.4:13251855-13251874 None:intergenic 45.0%
CGAAGAGATACCAGCATGAT+TGG + chr4.4:13252272-13252291 MS.gene023615:CDS 45.0%
CGAGAGATTGGATCAACTTG+AGG + chr4.4:13252570-13252589 MS.gene023615:CDS 45.0%
CTGCAATGCTTCAGAAACAG+AGG - chr4.4:13252374-13252393 None:intergenic 45.0%
GACGTTAAACACCGAGAGAT+TGG + chr4.4:13252558-13252577 MS.gene023615:CDS 45.0%
GAGACTCTTGCACATGAAGT+TGG + chr4.4:13252610-13252629 MS.gene023615:CDS 45.0%
GATACCAGCATGATTGGGAA+AGG + chr4.4:13252278-13252297 MS.gene023615:CDS 45.0%
GATGCACTCCACGAAACAAT+AGG - chr4.4:13251804-13251823 None:intergenic 45.0%
TGCTGTAAATCTCTTGCCAC+TGG + chr4.4:13252405-13252424 MS.gene023615:CDS 45.0%
TTAAAGCAACCACTTCTCGC+CGG - chr4.4:13252460-13252479 None:intergenic 45.0%
TTTCGTTGTGTGGAATCCTC+CGG + chr4.4:13251833-13251852 MS.gene023615:CDS 45.0%
! AAGTTCTCTGCCTCCTTTTC+TGG + chr4.4:13251740-13251759 MS.gene023615:CDS 45.0%
! CAACCTTCGTTTTCTCTCCA+TGG - chr4.4:13252437-13252456 None:intergenic 45.0%
! GTTATCCTCTGGATTTTCCC+TGG - chr4.4:13252530-13252549 None:intergenic 45.0%
!! AATGGTGGATGATCGATCCT+CGG + chr4.4:13251776-13251795 MS.gene023615:CDS 45.0%
AAAACCCTTGCGAATTCCGG+AGG - chr4.4:13251852-13251871 None:intergenic 50.0%
AATCACCACTGCAGCAACTG+GGG + chr4.4:13252819-13252838 MS.gene023615:CDS 50.0%
AGAATCACCACTGCAGCAAC+TGG + chr4.4:13252817-13252836 MS.gene023615:CDS 50.0%
ATTAACCCCAGTTGCTGCAG+TGG - chr4.4:13252827-13252846 None:intergenic 50.0%
CAGTTCTTGACAGAGGATCC+TGG + chr4.4:13252862-13252881 MS.gene023615:CDS 50.0%
CATGCCTTTCCCAATCATGC+TGG - chr4.4:13252285-13252304 None:intergenic 50.0%
CCACGAAACAATAGGATCCG+AGG - chr4.4:13251796-13251815 None:intergenic 50.0%
CCTCGGATCCTATTGTTTCG+TGG + chr4.4:13251793-13251812 MS.gene023615:CDS 50.0%
CCTTTCAGACTCCGTTAGAG+GGG - chr4.4:13252191-13252210 None:intergenic 50.0%
CTAACATGCCCCATCTGTTC+TGG - chr4.4:13253006-13253025 None:intergenic 50.0%
CTGGGAACAGTTCTTGACAG+AGG + chr4.4:13252855-13252874 MS.gene023615:CDS 50.0%
GAATCACCACTGCAGCAACT+GGG + chr4.4:13252818-13252837 MS.gene023615:CDS 50.0%
GAATCCTCCGGAATTCGCAA+GGG + chr4.4:13251845-13251864 MS.gene023615:CDS 50.0%
GCCTTTCAGACTCCGTTAGA+GGG - chr4.4:13252192-13252211 None:intergenic 50.0%
GGAGAGAAAACGAAGGTTGC+CGG + chr4.4:13252438-13252457 MS.gene023615:CDS 50.0%
GGATAGAGAACAACTCCTCG+TGG + chr4.4:13252246-13252265 MS.gene023615:CDS 50.0%
TCAAGCACAAGCCCCTCTAA+CGG + chr4.4:13252177-13252196 MS.gene023615:CDS 50.0%
TGCCTTTCAGACTCCGTTAG+AGG - chr4.4:13252193-13252212 None:intergenic 50.0%
TTGGCAAATTCCCACTGCTC+CGG - chr4.4:13252073-13252092 None:intergenic 50.0%
TTTGCCCAGGGAAAATCCAG+AGG + chr4.4:13252522-13252541 MS.gene023615:CDS 50.0%
! AGACTTCTGTAGCTTTGCCC+AGG + chr4.4:13252509-13252528 MS.gene023615:CDS 50.0%
! GAAAGTTGATCCGGAGCAGT+GGG + chr4.4:13252060-13252079 MS.gene023615:CDS 50.0%
! GACTTCTGTAGCTTTGCCCA+GGG + chr4.4:13252510-13252529 MS.gene023615:CDS 50.0%
!! TGAGGCATCCGTGGCATTTT+GGG + chr4.4:13252588-13252607 MS.gene023615:CDS 50.0%
!! TTGAGGCATCCGTGGCATTT+TGG + chr4.4:13252587-13252606 MS.gene023615:CDS 50.0%
CCCCTCTAACGGAGTCTGAA+AGG + chr4.4:13252188-13252207 MS.gene023615:CDS 55.0%
GACAGAGGATCCTGGTGCAT+CGG + chr4.4:13252870-13252889 MS.gene023615:CDS 55.0%
GGAATCCTCCGGAATTCGCA+AGG + chr4.4:13251844-13251863 MS.gene023615:CDS 55.0%
GGTATCTCTTCGTCTCCACG+AGG - chr4.4:13252264-13252283 None:intergenic 55.0%
! GGAAAGTTGATCCGGAGCAG+TGG + chr4.4:13252059-13252078 MS.gene023615:CDS 55.0%
! GGATCAACTTGAGGCATCCG+TGG + chr4.4:13252579-13252598 MS.gene023615:CDS 55.0%
ACCAGCTGCTGCTGCTCTTG+AGG + chr4.4:13252768-13252787 MS.gene023615:CDS 60.0%
CTCTCCATGGCTTCTGCCAG+TGG - chr4.4:13252424-13252443 None:intergenic 60.0%
CTTGCCACTGGCAGAAGCCA+TGG + chr4.4:13252417-13252436 MS.gene023615:CDS 60.0%
GCTGCAGTGGTGATTCTCGC+AGG - chr4.4:13252814-13252833 None:intergenic 60.0%
TCCTGTGCTTCCGATGCACC+AGG - chr4.4:13252883-13252902 None:intergenic 60.0%
! TCCTGGTGCATCGGAAGCAC+AGG + chr4.4:13252879-13252898 MS.gene023615:CDS 60.0%
CGAAGGTTGCCGGCGAGAAG+TGG + chr4.4:13252448-13252467 MS.gene023615:CDS 65.0%
GCCTCAAGAGCAGCAGCAGC+TGG - chr4.4:13252772-13252791 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 13251720 13253044 13251720 ID=MS.gene023615
chr4.4 mRNA 13251720 13253044 13251720 ID=MS.gene023615.t1;Parent=MS.gene023615
chr4.4 exon 13251720 13251932 13251720 ID=MS.gene023615.t1.exon1;Parent=MS.gene023615.t1
chr4.4 CDS 13251720 13251932 13251720 ID=cds.MS.gene023615.t1;Parent=MS.gene023615.t1
chr4.4 exon 13252052 13253044 13252052 ID=MS.gene023615.t1.exon2;Parent=MS.gene023615.t1
chr4.4 CDS 13252052 13253044 13252052 ID=cds.MS.gene023615.t1;Parent=MS.gene023615.t1
Gene Sequence

>MS.gene023615

ATGGATGAAGCTGGAAGTTCAAGTTCTCTGCCTCCTTTTCTGGCAAAGACTTATGAAATGGTGGATGATCGATCCTCGGATCCTATTGTTTCGTGGAGTGCATCGAACAAAAGTTTCGTTGTGTGGAATCCTCCGGAATTCGCAAGGGTTTTGCTTCCGAGATTCTTTAAGCACAATAACTTCTCCAGTTTTATCAGGCAGCTGAATACATATGTAAGCAAATTTGATGTCGGTGTTGTATTTGTTGATGCGAATTCTTGTTTTATGTTTATTTTATTTACTGATTCTGGTTTTTGGTTGCTTGAACTGATTCATAGTATATGTATGTGCAGGGATTTAGGAAAGTTGATCCGGAGCAGTGGGAATTTGCCAATGATGATTTTATAAGGGGGCAACCGCATCTGATGAAGAATATTCATAGACGGAAGCCCGTTCATAGTCATTCTCTGCATAATCTTCAAGCACAAGCCCCTCTAACGGAGTCTGAAAGGCAGAGTATGATTGATGAAATAGAGAAGCTGAAACAGGATAGAGAACAACTCCTCGTGGAGACGAAGAGATACCAGCATGATTGGGAAAGGCATGAGATTCAAATGCATTGTTCGAAAGATCAGTTGGAAAAATTGGAACATAAACAACAAAAGATGTTGTCCTCTGTTTCTGAAGCATTGCAGAAACCTATGATTGCTGTAAATCTCTTGCCACTGGCAGAAGCCATGGAGAGAAAACGAAGGTTGCCGGCGAGAAGTGGTTGCTTTAATAATGAAGCTAGTGTTGAAGATGCTATGGAGACTTCTGTAGCTTTGCCCAGGGAAAATCCAGAGGATAACTCTACTTTGACGTTAAACACCGAGAGATTGGATCAACTTGAGGCATCCGTGGCATTTTGGGAGACTCTTGCACATGAAGTTGGCGACAATTTCGTTCACACTCATTCAAACATGGATTTGGATGAATCTACATGCTGTGCTGATAGTTTGTCGATATCTTGTCAGCAACTAGACGGTGAAGTTCGACCTAAGTCACCTGAAATTGACATGAACGTTGAACCAGCTGCTGCTGCTCTTGAGGCTGTTGCAGTAAAAGAACAACCTGCGAGAATCACCACTGCAGCAACTGGGGTTAATGATGTATTCTGGGAACAGTTCTTGACAGAGGATCCTGGTGCATCGGAAGCACAGGAAGTGCAATCAGAAAGAAAAGATAATAGTAGCAGAAAAAATGAAGGAAAACCTAGTGATCATGGTAGATTTTGGTGGAACATGCGGAAATCAAACAATCATCCAGAACAGATGGGGCATGTTAGTCAAGTAGAGAAAATTTAG
Protein sequence

>MS.gene023615.t1

MDEAGSSSSLPPFLAKTYEMVDDRSSDPIVSWSASNKSFVVWNPPEFARVLLPRFFKHNNFSSFIRQLNTYGFRKVDPEQWEFANDDFIRGQPHLMKNIHRRKPVHSHSLHNLQAQAPLTESERQSMIDEIEKLKQDREQLLVETKRYQHDWERHEIQMHCSKDQLEKLEHKQQKMLSSVSEALQKPMIAVNLLPLAEAMERKRRLPARSGCFNNEASVEDAMETSVALPRENPEDNSTLTLNTERLDQLEASVAFWETLAHEVGDNFVHTHSNMDLDESTCCADSLSISCQQLDGEVRPKSPEIDMNVEPAAAALEAVAVKEQPARITTAATGVNDVFWEQFLTEDPGASEAQEVQSERKDNSSRKNEGKPSDHGRFWWNMRKSNNHPEQMGHVSQVEKI