AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066508

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066508.t1 MTR_3g104550 84.000 175 8 2 20 174 30 204 1.55e-102 304
MS.gene066508.t1 MTR_3g104550 85.915 142 20 0 145 286 237 378 3.65e-77 239
MS.gene066508.t1 MTR_7g095680 56.989 186 57 2 7 169 20 205 3.12e-68 216
MS.gene066508.t1 MTR_1g102860 59.639 166 48 3 23 169 27 192 8.73e-64 204
MS.gene066508.t1 MTR_5g029680 54.598 174 50 3 23 169 21 192 1.90e-59 197
MS.gene066508.t1 MTR_1g071360 60.127 158 50 2 23 169 19 174 1.10e-57 188
MS.gene066508.t1 MTR_1g071360 60.127 158 50 2 23 169 16 171 2.06e-57 188
MS.gene066508.t1 MTR_4g077970 46.500 200 83 3 35 210 11 210 8.06e-53 179
MS.gene066508.t1 MTR_1g061170 56.429 140 54 1 22 154 35 174 6.90e-48 166
MS.gene066508.t1 MTR_6g043060 44.118 170 73 2 36 183 22 191 1.62e-42 149
MS.gene066508.t1 MTR_8g087540 42.614 176 74 2 35 183 11 186 3.76e-41 147
MS.gene066508.t1 MTR_8g083100 54.918 122 50 1 37 153 23 144 5.50e-41 148
MS.gene066508.t1 MTR_2g100670 50.781 128 58 1 31 153 28 155 2.16e-40 142
MS.gene066508.t1 MTR_2g100680 50.400 125 57 1 34 153 29 153 2.47e-39 143
MS.gene066508.t1 MTR_2g100670 50.388 129 59 1 30 153 135 263 2.64e-39 142
MS.gene066508.t1 MTR_8g105780 51.493 134 49 2 36 153 22 155 2.94e-39 141
MS.gene066508.t1 MTR_2g017890 40.782 179 87 4 35 201 14 185 5.42e-39 140
MS.gene066508.t1 MTR_5g010680 61.856 97 37 0 34 130 22 118 2.09e-38 138
MS.gene066508.t1 MTR_3g101870 46.053 152 68 2 30 180 18 156 3.35e-37 135
MS.gene066508.t1 MTR_6g086805 61.290 93 36 0 36 128 11 103 8.22e-37 134
MS.gene066508.t1 MTR_7g091370 58.095 105 40 1 27 127 9 113 8.37e-36 128
MS.gene066508.t1 MTR_5g017470 57.895 95 40 0 33 127 6 100 2.13e-35 129
MS.gene066508.t1 MTR_0020s0020 58.511 94 39 0 34 127 31 124 4.84e-35 129
MS.gene066508.t1 MTR_1g100777 57.447 94 40 0 34 127 16 109 1.30e-34 127
MS.gene066508.t1 MTR_5g089170 60.638 94 37 0 34 127 23 116 3.91e-34 124
MS.gene066508.t1 MTR_4g088900 46.667 120 55 2 15 130 17 131 3.54e-30 113
MS.gene066508.t1 MTR_4g045757 50.746 67 33 0 27 93 5 71 1.06e-17 76.3
MS.gene066508.t1 MTR_5g047190 47.126 87 39 3 44 123 1 87 1.91e-15 70.9
MS.gene066508.t1 MTR_1g061170 47.059 68 29 1 94 154 12 79 1.04e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066508.t1 AT2G26150 46.372 317 108 11 23 284 31 340 6.24e-83 253
MS.gene066508.t1 AT2G26150 46.372 317 108 11 23 284 31 340 6.24e-83 253
MS.gene066508.t1 AT3G22830 53.333 180 57 2 23 175 48 227 1.93e-62 202
MS.gene066508.t1 AT5G16820 41.702 235 112 4 35 245 26 259 4.11e-55 185
MS.gene066508.t1 AT5G16820 41.702 235 112 4 35 245 26 259 4.11e-55 185
MS.gene066508.t1 AT3G51910 59.868 152 50 3 26 169 19 167 7.47e-55 179
MS.gene066508.t1 AT3G02990 43.056 216 101 3 35 228 22 237 1.27e-54 184
MS.gene066508.t1 AT1G32330 51.136 176 57 2 23 169 24 199 1.65e-54 184
MS.gene066508.t1 AT1G32330 51.136 176 57 2 23 169 24 199 1.93e-54 184
MS.gene066508.t1 AT4G17750 58.042 143 37 1 34 153 50 192 2.78e-54 183
MS.gene066508.t1 AT3G63350 69.565 115 35 0 12 126 4 118 5.25e-52 172
MS.gene066508.t1 AT5G43840 50.000 178 69 4 23 195 6 168 3.48e-49 164
MS.gene066508.t1 AT5G03720 50.000 164 64 3 2 153 22 179 1.14e-43 154
MS.gene066508.t1 AT5G03720 50.000 164 64 3 2 153 22 179 1.14e-43 154
MS.gene066508.t1 AT5G54070 45.783 166 85 2 29 189 63 228 2.79e-43 150
MS.gene066508.t1 AT3G24520 43.684 190 100 3 36 221 17 203 3.52e-41 145
MS.gene066508.t1 AT4G13980 41.279 172 83 3 37 200 24 185 1.64e-39 144
MS.gene066508.t1 AT1G67970 65.957 94 31 1 36 128 19 112 2.63e-39 141
MS.gene066508.t1 AT1G46264 58.824 102 42 0 26 127 24 125 2.09e-36 133
MS.gene066508.t1 AT2G41690 59.375 96 39 0 34 129 38 133 3.08e-36 130
MS.gene066508.t1 AT4G36990 52.778 108 48 1 34 138 12 119 2.15e-35 129
MS.gene066508.t1 AT5G62020 58.511 94 39 0 34 127 21 114 3.68e-35 129
MS.gene066508.t1 AT4G11660 58.511 94 39 0 34 127 57 150 9.20e-35 129
MS.gene066508.t1 AT2G26150 36.475 244 93 11 96 284 49 285 2.17e-31 118
MS.gene066508.t1 AT2G26150 36.475 244 93 11 96 284 49 285 2.17e-31 118
MS.gene066508.t1 AT5G45710 40.244 164 85 2 35 185 12 175 6.45e-31 118
MS.gene066508.t1 AT5G45710 40.244 164 85 2 35 185 12 175 6.45e-31 118
MS.gene066508.t1 AT4G18880 43.195 169 93 2 35 200 14 182 6.33e-30 117
MS.gene066508.t1 AT4G18870 42.045 88 51 0 36 123 146 233 8.47e-21 90.1
MS.gene066508.t1 AT4G18870 43.678 87 48 1 37 123 14 99 3.82e-18 82.8
MS.gene066508.t1 AT1G77570 36.667 90 54 2 37 123 27 116 3.78e-13 66.2

Find 73 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGTGCTTGTATTGAATT+AGG 0.170242 3.3:+85975946 MS.gene066508:CDS
CTCGTAATAGCTTTGTTGTT+TGG 0.240497 3.3:+85975411 MS.gene066508:CDS
AAGTGGTAATGAGAGTAATT+TGG 0.259305 3.3:+85976434 MS.gene066508:CDS
TTCAACAACATCAAAGGTTT+TGG 0.271463 3.3:-85975351 None:intergenic
TGCAATCCTTCCATTGGTTT+TGG 0.278910 3.3:-85975302 None:intergenic
TCGTAATAGCTTTGTTGTTT+GGG 0.292309 3.3:+85975412 MS.gene066508:CDS
AACTCTTCATCTTCCTCTTC+GGG 0.307939 3.3:+85975266 MS.gene066508:CDS
TGTATTGAATTAGGGGAGTA+TGG 0.320828 3.3:+85975955 MS.gene066508:CDS
TAACTCTTCATCTTCCTCTT+CGG 0.322245 3.3:+85975265 MS.gene066508:CDS
TGGTAGAAAAGAATGATAAT+TGG 0.355979 3.3:+85976565 MS.gene066508:CDS
GCAGAACCTTGTTGATCAAA+TGG 0.360342 3.3:+85976599 MS.gene066508:CDS
TGATGTGCCCGTGGAGGATT+TGG 0.372993 3.3:+85976545 MS.gene066508:CDS
TGAATCAAGCAGTGAAGTTA+AGG 0.388539 3.3:+85976386 MS.gene066508:CDS
AATGGAAGGATTGCACGAAA+TGG 0.400329 3.3:+85975310 MS.gene066508:CDS
AGTGGTAATGAGAGTAATTT+GGG 0.400460 3.3:+85976435 MS.gene066508:CDS
GTGGTGCTTGTATTGAATTA+GGG 0.410092 3.3:+85975947 MS.gene066508:CDS
CAGAACCTTGTTGATCAAAT+GGG 0.416106 3.3:+85976600 MS.gene066508:CDS
TTAGCATATGTCAGTGTTGT+TGG 0.420659 3.3:+85975229 None:intergenic
TTGAGAGGTTGAGAAGGGAT+AGG 0.448330 3.3:+85975992 MS.gene066508:CDS
TTAGGGGAGTATGGTCTTGA+AGG 0.455498 3.3:+85975964 MS.gene066508:CDS
TGAGATTGAGAGGTTGAGAA+GGG 0.457785 3.3:+85975987 MS.gene066508:CDS
TAGCATATGTCAGTGTTGTT+GGG 0.461007 3.3:+85975230 None:intergenic
TGAGAGGTTGAGAAGGGATA+GGG 0.461358 3.3:+85975993 MS.gene066508:CDS
GTGAGATTGAGAGGTTGAGA+AGG 0.465030 3.3:+85975986 MS.gene066508:CDS
GATTCCCACCAACCAACTCT+TGG 0.469023 3.3:-85976485 None:intergenic
ATGGAAGGATTGCACGAAAT+GGG 0.473051 3.3:+85975311 MS.gene066508:CDS
CCAATCACAACTTCATCCTC+TGG 0.476628 3.3:-85976507 None:intergenic
TAATCAACCACAGACTCAAT+TGG 0.480632 3.3:-85976288 None:intergenic
ATTTCGTGCAATCCTTCCAT+TGG 0.481224 3.3:-85975308 None:intergenic
GGCAGCAGCAACATAATTCA+AGG 0.488267 3.3:+85976046 MS.gene066508:intron
CGATGGGAGTTTGCGAACGA+AGG 0.501252 3.3:+85975836 MS.gene066508:CDS
AAGGGAGCAATTGTCTGCTA+TGG 0.507177 3.3:+85976065 MS.gene066508:CDS
TTCGCAAACTCCCATCGATC+CGG 0.510214 3.3:-85975830 None:intergenic
GACTACTATTGAGTCTGAGA+TGG 0.532873 3.3:+85976338 MS.gene066508:CDS
CTGAACCAAGAGTTGGTTGG+TGG 0.536158 3.3:+85976480 MS.gene066508:CDS
CAAAACCCATTTGATCAACA+AGG 0.537986 3.3:-85976605 None:intergenic
AAAAGTTGATCCGGATCGAT+GGG 0.538609 3.3:+85975820 MS.gene066508:CDS
GAAAAGTTGATCCGGATCGA+TGG 0.539674 3.3:+85975819 MS.gene066508:CDS
GATGGGAGTTTGCGAACGAA+GGG 0.539714 3.3:+85975837 MS.gene066508:CDS
CCACTGATTCCATTGTATCA+TGG 0.539990 3.3:+85975381 MS.gene066508:CDS
AATTGTCTGCTATGGAAGCT+AGG 0.540355 3.3:+85976073 MS.gene066508:CDS
GGATTTACTGAACCAAGAGT+TGG 0.543897 3.3:+85976473 MS.gene066508:CDS
TTTGGGTGATGCTGTGTGGG+AGG 0.547697 3.3:+85976452 MS.gene066508:CDS
CCAGAGGATGAAGTTGTGAT+TGG 0.548861 3.3:+85976507 MS.gene066508:CDS
TAATTTGGGTGATGCTGTGT+GGG 0.557732 3.3:+85976449 MS.gene066508:CDS
TGGATCTTCAACAACATCAA+AGG 0.559140 3.3:-85975357 None:intergenic
AAATTACTCTCATTACCACT+TGG 0.571022 3.3:-85976432 None:intergenic
AAATCTTACACCAAAACCAA+TGG 0.575232 3.3:+85975292 MS.gene066508:CDS
GTCTTGAAGGTGAGATTGAG+AGG 0.580331 3.3:+85975977 MS.gene066508:CDS
GGCTGTGCCAATTGAGTCTG+TGG 0.580769 3.3:+85976281 MS.gene066508:CDS
CTCTTGTCAAGAACAGCAAG+AGG 0.589029 3.3:+85976311 MS.gene066508:CDS
CCATGATACAATGGAATCAG+TGG 0.595116 3.3:-85975381 None:intergenic
AAGCTATGCAGCAAGAAACC+GGG 0.595926 3.3:+85975925 MS.gene066508:intron
TGAACCAAGAGTTGGTTGGT+GGG 0.615910 3.3:+85976481 MS.gene066508:CDS
GATACAATGGAATCAGTGGA+TGG 0.619245 3.3:-85975377 None:intergenic
AAAGAATGATAATTGGACTG+TGG 0.619356 3.3:+85976572 MS.gene066508:CDS
TGGTGCTTGTATTGAATTAG+GGG 0.620760 3.3:+85975948 MS.gene066508:CDS
TGTAAGATTTGAACCCGAAG+AGG 0.623342 3.3:-85975279 None:intergenic
TTCTCAAATTGATGTGCCCG+TGG 0.628790 3.3:+85976536 MS.gene066508:CDS
GTAACGAGGTAAAATAGCAG+TGG 0.630092 3.3:-85975450 None:intergenic
AGCTCCGCTCCATGATACAA+TGG 0.631104 3.3:-85975390 None:intergenic
CTTACACCAAAACCAATGGA+AGG 0.637289 3.3:+85975296 MS.gene066508:CDS
TCAAATTGATGTGCCCGTGG+AGG 0.639975 3.3:+85976539 MS.gene066508:CDS
AATTCAATACAAGCACCACC+CGG 0.644720 3.3:-85975943 None:intergenic
TTACTGAACCAAGAGTTGGT+TGG 0.648299 3.3:+85976477 MS.gene066508:CDS
GCAGCAGCAACATAATTCAA+GGG 0.652761 3.3:+85976047 MS.gene066508:intron
TCTTGTCAAGAACAGCAAGA+GGG 0.653894 3.3:+85976312 MS.gene066508:CDS
GATTCCATTGTATCATGGAG+CGG 0.654272 3.3:+85975386 MS.gene066508:CDS
GTAATTTGGGTGATGCTGTG+TGG 0.674970 3.3:+85976448 MS.gene066508:CDS
GTTGGTTGGTGGGAATCCAG+AGG 0.687367 3.3:+85976491 MS.gene066508:CDS
GCATTGAGTTCAGTTCCAAG+TGG 0.687922 3.3:+85976417 MS.gene066508:CDS
TTACTGTGTTTGAAGTAACG+AGG 0.690403 3.3:-85975464 None:intergenic
CTATGCAGCAAGAAACCGGG+TGG 0.725946 3.3:+85975928 MS.gene066508:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTAATAGTTAACTTACAT+AGG - chr3.3:85975519-85975538 None:intergenic 20.0%
! TGGTAGAAAAGAATGATAAT+TGG + chr3.3:85976565-85976584 MS.gene066508:CDS 25.0%
!! AATTTTTGTGTTCATATGCA+GGG + chr3.3:85975791-85975810 MS.gene066508:intron 25.0%
!! TCAATCTTTTATACAGCAAT+TGG + chr3.3:85976158-85976177 MS.gene066508:intron 25.0%
AAAGAATGATAATTGGACTG+TGG + chr3.3:85976572-85976591 MS.gene066508:CDS 30.0%
AAATCTTACACCAAAACCAA+TGG + chr3.3:85975292-85975311 MS.gene066508:CDS 30.0%
AAATTACTCTCATTACCACT+TGG - chr3.3:85976435-85976454 None:intergenic 30.0%
AAGAACAAATTCACAAACAC+CGG - chr3.3:85975728-85975747 None:intergenic 30.0%
GAGAATTTGCAAAATGATTC+TGG + chr3.3:85976250-85976269 MS.gene066508:intron 30.0%
TAATGGCTGAAATGTGAAAT+TGG + chr3.3:85976022-85976041 MS.gene066508:intron 30.0%
! AAGTGGTAATGAGAGTAATT+TGG + chr3.3:85976434-85976453 MS.gene066508:CDS 30.0%
! AGTGGTAATGAGAGTAATTT+GGG + chr3.3:85976435-85976454 MS.gene066508:CDS 30.0%
! GAATTTTTGTGTTCATATGC+AGG + chr3.3:85975790-85975809 MS.gene066508:intron 30.0%
! TTCAACAACATCAAAGGTTT+TGG - chr3.3:85975354-85975373 None:intergenic 30.0%
!! AAAATGATTCTGGAGCTTAA+GGG + chr3.3:85976260-85976279 MS.gene066508:intron 30.0%
!! TCGTAATAGCTTTGTTGTTT+GGG + chr3.3:85975412-85975431 MS.gene066508:CDS 30.0%
CAAAACCCATTTGATCAACA+AGG - chr3.3:85976608-85976627 None:intergenic 35.0%
CAGAACCTTGTTGATCAAAT+GGG + chr3.3:85976600-85976619 MS.gene066508:CDS 35.0%
CGTTGAAAATGAAGCGAAAA+CGG + chr3.3:85976203-85976222 MS.gene066508:intron 35.0%
GTGGTGCTTGTATTGAATTA+GGG + chr3.3:85975947-85975966 MS.gene066508:CDS 35.0%
TAACTCTTCATCTTCCTCTT+CGG + chr3.3:85975265-85975284 MS.gene066508:CDS 35.0%
TAATCAACCACAGACTCAAT+TGG - chr3.3:85976291-85976310 None:intergenic 35.0%
TGAATCAAGCAGTGAAGTTA+AGG + chr3.3:85976386-85976405 MS.gene066508:CDS 35.0%
TGGATCTTCAACAACATCAA+AGG - chr3.3:85975360-85975379 None:intergenic 35.0%
TGGTGCTTGTATTGAATTAG+GGG + chr3.3:85975948-85975967 MS.gene066508:CDS 35.0%
TGTATTGAATTAGGGGAGTA+TGG + chr3.3:85975955-85975974 MS.gene066508:CDS 35.0%
! GATTACTAAGTGCTTTTGCA+AGG - chr3.3:85976140-85976159 None:intergenic 35.0%
! TTACTGTGTTTGAAGTAACG+AGG - chr3.3:85975467-85975486 None:intergenic 35.0%
!! CAAAATGATTCTGGAGCTTA+AGG + chr3.3:85976259-85976278 MS.gene066508:intron 35.0%
!! CTCGTAATAGCTTTGTTGTT+TGG + chr3.3:85975411-85975430 MS.gene066508:CDS 35.0%
!! TCAAAGGTTTTGGTGAGAAA+TGG - chr3.3:85975344-85975363 None:intergenic 35.0%
!!! GGGTTTTAGAAAAGTTGATC+CGG + chr3.3:85975811-85975830 MS.gene066508:intron 35.0%
AACTCTTCATCTTCCTCTTC+GGG + chr3.3:85975266-85975285 MS.gene066508:CDS 40.0%
AATGGAAGGATTGCACGAAA+TGG + chr3.3:85975310-85975329 MS.gene066508:CDS 40.0%
AATTCAATACAAGCACCACC+CGG - chr3.3:85975946-85975965 None:intergenic 40.0%
AATTGTCTGCTATGGAAGCT+AGG + chr3.3:85976073-85976092 MS.gene066508:CDS 40.0%
ATGGAAGGATTGCACGAAAT+GGG + chr3.3:85975311-85975330 MS.gene066508:CDS 40.0%
ATTTCGTGCAATCCTTCCAT+TGG - chr3.3:85975311-85975330 None:intergenic 40.0%
CCAGACAATGTTGATGTATC+CGG + chr3.3:85975706-85975725 MS.gene066508:intron 40.0%
CCATGATACAATGGAATCAG+TGG - chr3.3:85975384-85975403 None:intergenic 40.0%
CTTACACCAAAACCAATGGA+AGG + chr3.3:85975296-85975315 MS.gene066508:CDS 40.0%
GACTACTATTGAGTCTGAGA+TGG + chr3.3:85976338-85976357 MS.gene066508:CDS 40.0%
GATACAATGGAATCAGTGGA+TGG - chr3.3:85975380-85975399 None:intergenic 40.0%
GATTCCATTGTATCATGGAG+CGG + chr3.3:85975386-85975405 MS.gene066508:CDS 40.0%
GCACAGAATAGAGAGTTGAA+AGG + chr3.3:85976180-85976199 MS.gene066508:intron 40.0%
GCAGAACCTTGTTGATCAAA+TGG + chr3.3:85976599-85976618 MS.gene066508:CDS 40.0%
GCAGCAGCAACATAATTCAA+GGG + chr3.3:85976047-85976066 MS.gene066508:intron 40.0%
GGATTTACTGAACCAAGAGT+TGG + chr3.3:85976473-85976492 MS.gene066508:CDS 40.0%
GGTGGTGCTTGTATTGAATT+AGG + chr3.3:85975946-85975965 MS.gene066508:CDS 40.0%
GTAACGAGGTAAAATAGCAG+TGG - chr3.3:85975453-85975472 None:intergenic 40.0%
TAATTTGGGTGATGCTGTGT+GGG + chr3.3:85976449-85976468 MS.gene066508:CDS 40.0%
TCTTGTCAAGAACAGCAAGA+GGG + chr3.3:85976312-85976331 MS.gene066508:CDS 40.0%
TGAAAATGAAGCGAAAACGG+AGG + chr3.3:85976206-85976225 MS.gene066508:intron 40.0%
TGAGATTGAGAGGTTGAGAA+GGG + chr3.3:85975987-85976006 MS.gene066508:CDS 40.0%
TGGCTGAAATGTGAAATTGG+AGG + chr3.3:85976025-85976044 MS.gene066508:intron 40.0%
TGTAAGATTTGAACCCGAAG+AGG - chr3.3:85975282-85975301 None:intergenic 40.0%
! AAAAGTTGATCCGGATCGAT+GGG + chr3.3:85975820-85975839 MS.gene066508:CDS 40.0%
! CTTTTCTACCAAATCCTCCA+CGG - chr3.3:85976556-85976575 None:intergenic 40.0%
! TTACTGAACCAAGAGTTGGT+TGG + chr3.3:85976477-85976496 MS.gene066508:CDS 40.0%
! TTTTCTACCAAATCCTCCAC+GGG - chr3.3:85976555-85976574 None:intergenic 40.0%
!! AAGGGATAGGGCTGTTTTAA+TGG + chr3.3:85976005-85976024 MS.gene066508:intron 40.0%
!! AAGGTTTTGGTGAGAAATGG+AGG - chr3.3:85975341-85975360 None:intergenic 40.0%
!! CCACTGATTCCATTGTATCA+TGG + chr3.3:85975381-85975400 MS.gene066508:CDS 40.0%
!!! TGCAATCCTTCCATTGGTTT+TGG - chr3.3:85975305-85975324 None:intergenic 40.0%
AAGCTATGCAGCAAGAAACC+GGG + chr3.3:85975925-85975944 MS.gene066508:intron 45.0%
AAGGGAGCAATTGTCTGCTA+TGG + chr3.3:85976065-85976084 MS.gene066508:CDS 45.0%
CAAGCTATGCAGCAAGAAAC+CGG + chr3.3:85975924-85975943 MS.gene066508:intron 45.0%
CCAATCACAACTTCATCCTC+TGG - chr3.3:85976510-85976529 None:intergenic 45.0%
CCAGAGGATGAAGTTGTGAT+TGG + chr3.3:85976507-85976526 MS.gene066508:CDS 45.0%
CCGGATACATCAACATTGTC+TGG - chr3.3:85975709-85975728 None:intergenic 45.0%
CTCTTGTCAAGAACAGCAAG+AGG + chr3.3:85976311-85976330 MS.gene066508:CDS 45.0%
GCATTGAGTTCAGTTCCAAG+TGG + chr3.3:85976417-85976436 MS.gene066508:CDS 45.0%
GGCAGCAGCAACATAATTCA+AGG + chr3.3:85976046-85976065 MS.gene066508:intron 45.0%
GTAATTTGGGTGATGCTGTG+TGG + chr3.3:85976448-85976467 MS.gene066508:CDS 45.0%
GTCTTGAAGGTGAGATTGAG+AGG + chr3.3:85975977-85975996 MS.gene066508:CDS 45.0%
GTGAGATTGAGAGGTTGAGA+AGG + chr3.3:85975986-85976005 MS.gene066508:CDS 45.0%
TGAGAGGTTGAGAAGGGATA+GGG + chr3.3:85975993-85976012 MS.gene066508:CDS 45.0%
TTCTCAAATTGATGTGCCCG+TGG + chr3.3:85976536-85976555 MS.gene066508:CDS 45.0%
TTGAGAGGTTGAGAAGGGAT+AGG + chr3.3:85975992-85976011 MS.gene066508:CDS 45.0%
! GAAAAGTTGATCCGGATCGA+TGG + chr3.3:85975819-85975838 MS.gene066508:CDS 45.0%
! TGAACCAAGAGTTGGTTGGT+GGG + chr3.3:85976481-85976500 MS.gene066508:CDS 45.0%
! TTAGGGGAGTATGGTCTTGA+AGG + chr3.3:85975964-85975983 MS.gene066508:CDS 45.0%
!! GTTTTGGTGAGAAATGGAGG+TGG - chr3.3:85975338-85975357 None:intergenic 45.0%
!!! CGAACGAAGGGTTTTTAGCT+GGG + chr3.3:85975849-85975868 MS.gene066508:CDS 45.0%
AGCTCCGCTCCATGATACAA+TGG - chr3.3:85975393-85975412 None:intergenic 50.0%
GATGGGAGTTTGCGAACGAA+GGG + chr3.3:85975837-85975856 MS.gene066508:CDS 50.0%
GATTCCCACCAACCAACTCT+TGG - chr3.3:85976488-85976507 None:intergenic 50.0%
TCAAATTGATGTGCCCGTGG+AGG + chr3.3:85976539-85976558 MS.gene066508:CDS 50.0%
TTCGCAAACTCCCATCGATC+CGG - chr3.3:85975833-85975852 None:intergenic 50.0%
! CTGAACCAAGAGTTGGTTGG+TGG + chr3.3:85976480-85976499 MS.gene066508:CDS 50.0%
! GCTCACTGCTAGTTCGAGTT+TGG + chr3.3:85976228-85976247 MS.gene066508:intron 50.0%
!!! AAGGGTTTTTAGCTGGGCAG+AGG + chr3.3:85975855-85975874 MS.gene066508:CDS 50.0%
!!! GCGAACGAAGGGTTTTTAGC+TGG + chr3.3:85975848-85975867 MS.gene066508:CDS 50.0%
CGATGGGAGTTTGCGAACGA+AGG + chr3.3:85975836-85975855 MS.gene066508:CDS 55.0%
CTATGCAGCAAGAAACCGGG+TGG + chr3.3:85975928-85975947 MS.gene066508:intron 55.0%
GGCTGTGCCAATTGAGTCTG+TGG + chr3.3:85976281-85976300 MS.gene066508:CDS 55.0%
TGATGTGCCCGTGGAGGATT+TGG + chr3.3:85976545-85976564 MS.gene066508:CDS 55.0%
TTTGGGTGATGCTGTGTGGG+AGG + chr3.3:85976452-85976471 MS.gene066508:CDS 55.0%
!! GTTGGTTGGTGGGAATCCAG+AGG + chr3.3:85976491-85976510 MS.gene066508:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 85975236 85976635 85975236 ID=MS.gene066508
chr3.3 mRNA 85975236 85976635 85975236 ID=MS.gene066508.t1;Parent=MS.gene066508
chr3.3 exon 85975236 85975520 85975236 ID=MS.gene066508.t1.exon1;Parent=MS.gene066508.t1
chr3.3 CDS 85975236 85975520 85975236 ID=cds.MS.gene066508.t1;Parent=MS.gene066508.t1
chr3.3 exon 85975812 85975910 85975812 ID=MS.gene066508.t1.exon2;Parent=MS.gene066508.t1
chr3.3 CDS 85975812 85975910 85975812 ID=cds.MS.gene066508.t1;Parent=MS.gene066508.t1
chr3.3 exon 85975940 85976017 85975940 ID=MS.gene066508.t1.exon3;Parent=MS.gene066508.t1
chr3.3 CDS 85975940 85976017 85975940 ID=cds.MS.gene066508.t1;Parent=MS.gene066508.t1
chr3.3 exon 85976051 85976094 85976051 ID=MS.gene066508.t1.exon4;Parent=MS.gene066508.t1
chr3.3 CDS 85976051 85976094 85976051 ID=cds.MS.gene066508.t1;Parent=MS.gene066508.t1
chr3.3 exon 85976281 85976635 85976281 ID=MS.gene066508.t1.exon5;Parent=MS.gene066508.t1
chr3.3 CDS 85976281 85976635 85976281 ID=cds.MS.gene066508.t1;Parent=MS.gene066508.t1
Gene Sequence

>MS.gene066508

ATGTCAGTGTTGTTGGGTTCAACATCATGTAACTCTTCATCTTCCTCTTCGGGTTCAAATCTTACACCAAAACCAATGGAAGGATTGCACGAAATGGGTCCACCTCCATTTCTCACCAAAACCTTTGATGTTGTTGAAGATCCATCCACTGATTCCATTGTATCATGGAGCGGAGCTCGTAATAGCTTTGTTGTTTGGGATCTTCACAAATTCTCCACTGCTATTTTACCTCGTTACTTCAAACACAGTAATTTCTCTAGCTTTGTTCGACAACTCAATACCTATGTAAGTTAACTATTACTTTTTCTTTTTGAATTTGATTTTTATTTCGTTGTTGTTAAAATTAGTTCAATATTGTTCAACTTAGATACGAGTTAGTTATAATTAGTTGAAAATTTGTTATTATTATTAGTTAGCTGATTTAGACTGACACATATGATTACATTCAATTATTTTGATGTATGTCAGTGCCAGACAATGTTGATGTATCCGGTGTTTGTGAATTTGTTCTTGCTTATCAAACTAAGATGTTTTTTATGTTCAATTTGTTGAGTGAATTTTTGTGTTCATATGCAGGGTTTTAGAAAAGTTGATCCGGATCGATGGGAGTTTGCGAACGAAGGGTTTTTAGCTGGGCAGAGGAACTTGTTGAAAACAATCAAGAGAAGAAGAAATGTGACACAAATCACAAGCTATGCAGCAAGAAACCGGGTGGTGCTTGTATTGAATTAGGGGAGTATGGTCTTGAAGGTGAGATTGAGAGGTTGAGAAGGGATAGGGCTGTTTTAATGGCTGAAATGTGAAATTGGAGGCAGCAGCAACATAATTCAAGGGAGCAATTGTCTGCTATGGAAGCTAGGTTATTGATTACTGAGAAGAAACATCAACAGATGATGAATTTCCTTGCAAAAGCACTTAGTAATCAATCTTTTATACAGCAATTGGCACAGAATAGAGAGTTGAAAGGCGTTGAAAATGAAGCGAAAACGGAGGCTCACTGCTAGTTCGAGTTTGGAGAATTTGCAAAATGATTCTGGAGCTTAAGGGCTGTGCCAATTGAGTCTGTGGTTGATTACTCTTGTCAAGAACAGCAAGAGGGTTTGACTACTATTGAGTCTGAGATGGAGACACTGTTAAGTGCTTATGATAATGAATCAAGCAGTGAAGTTAAGGACTACACTGCATTGAGTTCAGTTCCAAGTGGTAATGAGAGTAATTTGGGTGATGCTGTGTGGGAGGATTTACTGAACCAAGAGTTGGTTGGTGGGAATCCAGAGGATGAAGTTGTGATTGGTGATTTTTCTCAAATTGATGTGCCCGTGGAGGATTTGGTAGAAAAGAATGATAATTGGACTGTGGATTTGCAGAACCTTGTTGATCAAATGGGTTTTGAACCTTAG
Protein sequence

>MS.gene066508.t1

MSVLLGSTSCNSSSSSSGSNLTPKPMEGLHEMGPPPFLTKTFDVVEDPSTDSIVSWSGARNSFVVWDLHKFSTAILPRYFKHSNFSSFVRQLNTYGFRKVDPDRWEFANEGFLAGQRNLLKTIKRRRNKPGGACIELGEYGLEGEIERLRRDRAQQHNSREQLSAMEARAVPIESVVDYSCQEQQEGLTTIESEMETLLSAYDNESSSEVKDYTALSSVPSGNESNLGDAVWEDLLNQELVGGNPEDEVVIGDFSQIDVPVEDLVEKNDNWTVDLQNLVDQMGFEP