AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32806

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32806.t1 MTR_3g104550 96.850 381 9 1 1 381 1 378 0.0 759
MS.gene32806.t1 MTR_7g095680 56.126 253 103 2 8 252 8 260 6.04e-93 283
MS.gene32806.t1 MTR_1g102860 62.500 216 74 4 36 246 27 240 6.17e-91 276
MS.gene32806.t1 MTR_5g029680 54.911 224 85 4 36 247 21 240 8.97e-80 253
MS.gene32806.t1 MTR_1g071360 44.224 303 143 8 36 331 19 302 5.70e-73 231
MS.gene32806.t1 MTR_1g071360 44.224 303 143 8 36 331 16 299 1.32e-72 231
MS.gene32806.t1 MTR_4g077970 54.327 208 87 2 48 247 11 218 2.29e-72 234
MS.gene32806.t1 MTR_1g061170 45.161 279 147 4 35 310 35 310 1.32e-71 231
MS.gene32806.t1 MTR_6g086805 45.274 201 106 2 49 249 11 207 6.08e-53 179
MS.gene32806.t1 MTR_8g105780 46.392 194 97 2 49 235 22 215 6.18e-50 172
MS.gene32806.t1 MTR_8g087540 42.308 208 116 1 48 255 11 214 2.46e-48 169
MS.gene32806.t1 MTR_2g100680 39.604 202 117 2 47 247 29 226 5.65e-48 169
MS.gene32806.t1 MTR_2g100670 41.146 192 109 1 44 235 28 215 5.23e-46 160
MS.gene32806.t1 MTR_6g043060 42.365 203 107 5 49 248 22 217 6.99e-46 160
MS.gene32806.t1 MTR_8g083100 43.284 201 104 5 50 247 23 216 7.88e-46 164
MS.gene32806.t1 MTR_2g100670 40.933 193 110 1 43 235 135 323 3.62e-45 160
MS.gene32806.t1 MTR_2g017890 41.243 177 101 1 48 221 14 190 2.44e-43 155
MS.gene32806.t1 MTR_5g010680 62.766 94 35 0 47 140 22 115 6.59e-38 140
MS.gene32806.t1 MTR_7g091370 45.062 162 76 3 40 188 9 170 2.50e-37 135
MS.gene32806.t1 MTR_3g101870 56.757 111 43 1 43 153 18 123 1.05e-36 137
MS.gene32806.t1 MTR_5g017470 55.882 102 45 0 46 147 6 107 1.13e-36 135
MS.gene32806.t1 MTR_0020s0020 58.511 94 39 0 47 140 31 124 1.22e-34 130
MS.gene32806.t1 MTR_1g100777 54.206 107 49 0 47 153 16 122 1.56e-34 129
MS.gene32806.t1 MTR_1g061170 38.537 205 120 4 109 310 14 215 5.80e-34 129
MS.gene32806.t1 MTR_5g089170 60.638 94 37 0 47 140 23 116 9.10e-34 126
MS.gene32806.t1 MTR_4g088900 36.957 184 101 5 28 204 17 192 7.22e-29 112
MS.gene32806.t1 MTR_4g045757 50.746 67 33 0 40 106 5 71 8.43e-18 77.8
MS.gene32806.t1 MTR_5g047190 45.977 87 40 2 57 136 1 87 3.59e-15 71.2
MS.gene32806.t1 MTR_2g043940 32.168 143 79 5 85 221 7 137 1.88e-11 65.5
MS.gene32806.t1 MTR_6g044340 29.299 157 101 4 95 248 8 157 1.98e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32806.t1 AT2G26150 52.125 353 117 7 36 379 31 340 3.14e-120 352
MS.gene32806.t1 AT2G26150 52.125 353 117 7 36 379 31 340 3.14e-120 352
MS.gene32806.t1 AT3G22830 43.978 357 159 8 36 359 48 396 8.09e-88 271
MS.gene32806.t1 AT3G51910 55.357 224 79 3 39 256 19 227 1.50e-76 238
MS.gene32806.t1 AT4G17750 51.121 223 92 2 47 252 50 272 2.41e-75 242
MS.gene32806.t1 AT1G32330 46.931 277 122 7 15 274 3 271 2.30e-74 239
MS.gene32806.t1 AT1G32330 46.931 277 122 7 15 274 3 271 2.43e-74 239
MS.gene32806.t1 AT5G16820 50.218 229 98 3 27 244 10 233 6.25e-74 238
MS.gene32806.t1 AT5G16820 50.218 229 98 3 27 244 10 233 6.25e-74 238
MS.gene32806.t1 AT3G02990 43.929 280 141 6 48 318 22 294 8.56e-73 234
MS.gene32806.t1 AT3G63350 43.175 315 119 7 25 331 4 266 1.46e-69 220
MS.gene32806.t1 AT5G43840 43.011 279 125 5 36 297 6 267 1.98e-68 217
MS.gene32806.t1 AT2G26150 44.484 281 102 8 109 379 49 285 3.03e-67 214
MS.gene32806.t1 AT2G26150 44.484 281 102 8 109 379 49 285 3.03e-67 214
MS.gene32806.t1 AT5G03720 45.815 227 111 4 17 240 24 241 1.25e-60 201
MS.gene32806.t1 AT5G03720 45.815 227 111 4 17 240 24 241 1.25e-60 201
MS.gene32806.t1 AT1G67970 47.340 188 98 1 49 235 19 206 2.15e-55 186
MS.gene32806.t1 AT5G54070 42.857 217 109 3 42 257 63 265 2.63e-55 185
MS.gene32806.t1 AT4G13980 40.201 199 114 2 50 247 24 218 8.80e-48 169
MS.gene32806.t1 AT3G24520 45.894 207 85 5 49 251 17 200 2.34e-47 164
MS.gene32806.t1 AT5G45710 31.419 296 184 5 48 333 12 298 2.32e-37 138
MS.gene32806.t1 AT5G45710 31.419 296 184 5 48 333 12 298 2.32e-37 138
MS.gene32806.t1 AT4G36990 52.632 114 48 1 47 160 12 119 1.48e-36 134
MS.gene32806.t1 AT1G46264 38.191 199 70 3 39 187 24 219 2.94e-36 135
MS.gene32806.t1 AT2G41690 48.889 135 52 2 6 140 14 131 6.21e-36 132
MS.gene32806.t1 AT4G18880 44.000 175 97 1 48 221 14 188 1.34e-35 134
MS.gene32806.t1 AT5G62020 55.882 102 45 0 47 148 21 122 1.90e-35 132
MS.gene32806.t1 AT4G11660 58.511 94 39 0 47 140 57 150 2.10e-34 131
MS.gene32806.t1 AT4G18870 34.711 121 75 1 49 165 146 266 1.33e-20 91.3
MS.gene32806.t1 AT4G18870 43.678 87 48 1 50 136 14 99 6.43e-18 83.6
MS.gene32806.t1 AT1G77570 36.667 90 54 2 50 136 27 116 6.25e-13 66.2

Find 82 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGTGCTTGTATTGAATT+AGG 0.178257 3.1:+82444232 MS.gene32806:CDS
GAGAATTTGCAAAATGATTC+TGG 0.205764 3.1:+82444535 MS.gene32806:CDS
CTCGTAATAGCTTTGTTGTT+TGG 0.240497 3.1:+82443711 MS.gene32806:CDS
AAGTGGTAATGAGAGTAATT+TGG 0.259305 3.1:+82444720 MS.gene32806:CDS
TTCAACAACATCAAAGGTTT+TGG 0.271463 3.1:-82443651 None:intergenic
TGCAATCCTTCCATTGGTTT+TGG 0.278910 3.1:-82443602 None:intergenic
CCACTGATTCCATTGTTTCA+TGG 0.288131 3.1:+82443681 MS.gene32806:CDS
TCGTAATAGCTTTGTTGTTT+GGG 0.292309 3.1:+82443712 MS.gene32806:CDS
AACTCTTCATCTTCCTCTTC+GGG 0.307939 3.1:+82443566 MS.gene32806:CDS
TGTATTGAATTAGGGGAGTA+TGG 0.320828 3.1:+82444241 MS.gene32806:CDS
TAACTCTTCATCTTCCTCTT+CGG 0.322245 3.1:+82443565 MS.gene32806:CDS
TGGTAGAAAAGAATGATAAT+TGG 0.355979 3.1:+82444851 MS.gene32806:CDS
GCAGAACCTTGTTGATCAAA+TGG 0.360342 3.1:+82444885 MS.gene32806:CDS
GCTCACTGCTAGTTCGAGTT+TGG 0.362423 3.1:+82444513 MS.gene32806:CDS
TTAGCATATGTCAGTGTTGT+TGG 0.370710 3.1:+82443530 MS.gene32806:CDS
TGATGTGCCCGTGGAGGATT+TGG 0.372993 3.1:+82444831 MS.gene32806:CDS
GTGGTGCTTGTATTGAATTA+GGG 0.384837 3.1:+82444233 MS.gene32806:CDS
TGAATCAAGCAGTGAAGTTA+AGG 0.388539 3.1:+82444672 MS.gene32806:CDS
AAAATGATTCTGGAGCTATA+AGG 0.395328 3.1:+82444545 MS.gene32806:CDS
AATGGAAGGATTGCACGAAA+TGG 0.400329 3.1:+82443610 MS.gene32806:CDS
AGTGGTAATGAGAGTAATTT+GGG 0.400460 3.1:+82444721 MS.gene32806:CDS
CAGAACCTTGTTGATCAAAT+GGG 0.416106 3.1:+82444886 MS.gene32806:CDS
TGGCTGAGATTGTGAAGTTA+AGG 0.421096 3.1:+82444311 MS.gene32806:CDS
AAATGATTCTGGAGCTATAA+GGG 0.430722 3.1:+82444546 MS.gene32806:CDS
TTAGGGGAGTATGGTCTTGA+AGG 0.455498 3.1:+82444250 MS.gene32806:CDS
GAAGGGAATTAGAGTGAAAG+AGG 0.460183 3.1:+82443499 MS.gene32806:CDS
GTGAGATTGAGAGGTTGAGA+AGG 0.465030 3.1:+82444272 MS.gene32806:CDS
GATTCCCACCAACCAACTCT+TGG 0.469023 3.1:-82444771 None:intergenic
ATGGAAGGATTGCACGAAAT+GGG 0.473051 3.1:+82443611 MS.gene32806:CDS
CCAATCACAACTTCATCCTC+TGG 0.476628 3.1:-82444793 None:intergenic
TAATCAACCACAGACTCAAT+TGG 0.480632 3.1:-82444574 None:intergenic
ATTTCGTGCAATCCTTCCAT+TGG 0.481224 3.1:-82443608 None:intergenic
GCGTTGAAATGAAGCGAAAA+CGG 0.487154 3.1:+82444488 MS.gene32806:CDS
TGAGATTGAGAGGTTGAGAA+GGG 0.495911 3.1:+82444273 MS.gene32806:CDS
AAGGGAGCAATTATCTGCTA+TGG 0.496135 3.1:+82444351 MS.gene32806:CDS
GGCAGCAGCAGCATAATTCA+AGG 0.500409 3.1:+82444332 MS.gene32806:CDS
CGATGGGAGTTTGCGAACGA+AGG 0.501252 3.1:+82444124 MS.gene32806:CDS
TTGAGAGGTTGAGAAGGGAC+AGG 0.504469 3.1:+82444278 MS.gene32806:CDS
TTCGCAAACTCCCATCGATC+CGG 0.510214 3.1:-82444118 None:intergenic
AATTATCTGCTATGGAAGCT+AGG 0.522811 3.1:+82444359 MS.gene32806:CDS
GACTACTATTGAGTCTGAGA+TGG 0.532873 3.1:+82444624 MS.gene32806:CDS
CTGAACCAAGAGTTGGTTGG+TGG 0.536158 3.1:+82444766 MS.gene32806:CDS
CAAAACCCATTTGATCAACA+AGG 0.537986 3.1:-82444891 None:intergenic
AAAAGTTGATCCGGATCGAT+GGG 0.538609 3.1:+82444108 MS.gene32806:CDS
GAAAAGTTGATCCGGATCGA+TGG 0.539674 3.1:+82444107 MS.gene32806:CDS
GATGGGAGTTTGCGAACGAA+GGG 0.539714 3.1:+82444125 MS.gene32806:CDS
CAAGCTATGCAGCAAGAGAC+CGG 0.541548 3.1:+82444211 MS.gene32806:CDS
GGATTTACTGAACCAAGAGT+TGG 0.543897 3.1:+82444759 MS.gene32806:CDS
TTTGGGTGATGCTGTGTGGG+AGG 0.547697 3.1:+82444738 MS.gene32806:CDS
CCAGAGGATGAAGTTGTGAT+TGG 0.548861 3.1:+82444793 MS.gene32806:CDS
TAATTTGGGTGATGCTGTGT+GGG 0.557732 3.1:+82444735 MS.gene32806:CDS
TGGATCTTCAACAACATCAA+AGG 0.559140 3.1:-82443657 None:intergenic
AAATTACTCTCATTACCACT+TGG 0.571022 3.1:-82444718 None:intergenic
AAATCTTACACCAAAACCAA+TGG 0.575232 3.1:+82443592 MS.gene32806:CDS
GTCTTGAAGGTGAGATTGAG+AGG 0.580331 3.1:+82444263 MS.gene32806:CDS
GGCTGTGCCAATTGAGTCTG+TGG 0.580769 3.1:+82444567 MS.gene32806:CDS
CTCTTGTCAAGAACAGCAAG+AGG 0.589029 3.1:+82444597 MS.gene32806:CDS
CCATGAAACAATGGAATCAG+TGG 0.596228 3.1:-82443681 None:intergenic
GAAACAATGGAATCAGTGGA+TGG 0.602123 3.1:-82443677 None:intergenic
TGGTGCTTGTATTGAATTAG+GGG 0.604898 3.1:+82444234 MS.gene32806:CDS
GCATCAACTAACTCAATGAA+GGG 0.610881 3.1:+82443482 None:intergenic
TGAGAGGTTGAGAAGGGACA+GGG 0.615785 3.1:+82444279 MS.gene32806:CDS
TGAACCAAGAGTTGGTTGGT+GGG 0.615910 3.1:+82444767 MS.gene32806:CDS
TAATTCAATACAAGCACCAC+CGG 0.616513 3.1:-82444230 None:intergenic
GATTCCATTGTTTCATGGAG+CGG 0.618876 3.1:+82443686 MS.gene32806:CDS
AAAGAATGATAATTGGACTG+TGG 0.619356 3.1:+82444858 MS.gene32806:CDS
TGTAAGATTTGAACCCGAAG+AGG 0.623342 3.1:-82443579 None:intergenic
GCAGCAGCAGCATAATTCAA+GGG 0.624420 3.1:+82444333 MS.gene32806:CDS
TTCTCAAATTGATGTGCCCG+TGG 0.628790 3.1:+82444822 MS.gene32806:CDS
GTAACGAGGTAAAATAGCAG+TGG 0.630092 3.1:-82443750 None:intergenic
AGCTCCGCTCCATGAAACAA+TGG 0.631104 3.1:-82443690 None:intergenic
CTTACACCAAAACCAATGGA+AGG 0.637289 3.1:+82443596 MS.gene32806:CDS
TCAAATTGATGTGCCCGTGG+AGG 0.639975 3.1:+82444825 MS.gene32806:CDS
GCACATAATAGAGAGTTGAA+AGG 0.641025 3.1:+82444466 MS.gene32806:CDS
TTACTGAACCAAGAGTTGGT+TGG 0.648299 3.1:+82444763 MS.gene32806:CDS
TCTTGTCAAGAACAGCAAGA+GGG 0.653894 3.1:+82444598 MS.gene32806:CDS
GTAATTTGGGTGATGCTGTG+TGG 0.674970 3.1:+82444734 MS.gene32806:CDS
TTGAAATGAAGCGAAAACGG+AGG 0.685238 3.1:+82444491 MS.gene32806:CDS
GTTGGTTGGTGGGAATCCAG+AGG 0.687367 3.1:+82444777 MS.gene32806:CDS
GCATTGAGTTCAGTTCCAAG+TGG 0.687922 3.1:+82444703 MS.gene32806:CDS
TTACTGTGTTTGAAGTAACG+AGG 0.690403 3.1:-82443764 None:intergenic
GCTATGCAGCAAGAGACCGG+TGG 0.749743 3.1:+82444214 MS.gene32806:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTAATAGTTAACTTACAT+AGG - chr3.1:82443819-82443838 None:intergenic 20.0%
!!! TCAATCTTTTATACAACAAT+TGG + chr3.1:82444444-82444463 MS.gene32806:CDS 20.0%
! TGGTAGAAAAGAATGATAAT+TGG + chr3.1:82444851-82444870 MS.gene32806:CDS 25.0%
! TTGAATGTAATCATATGTGT+CGG - chr3.1:82443952-82443971 None:intergenic 25.0%
!! AATTTTTGTGTTCATATGCA+GGG + chr3.1:82444079-82444098 MS.gene32806:intron 25.0%
AAAGAATGATAATTGGACTG+TGG + chr3.1:82444858-82444877 MS.gene32806:CDS 30.0%
AAATCTTACACCAAAACCAA+TGG + chr3.1:82443592-82443611 MS.gene32806:CDS 30.0%
AAATTACTCTCATTACCACT+TGG - chr3.1:82444721-82444740 None:intergenic 30.0%
AAGAACAAATTCACAAACAC+CGG - chr3.1:82444015-82444034 None:intergenic 30.0%
GAGAATTTGCAAAATGATTC+TGG + chr3.1:82444535-82444554 MS.gene32806:CDS 30.0%
! AAGTGGTAATGAGAGTAATT+TGG + chr3.1:82444720-82444739 MS.gene32806:CDS 30.0%
! AGTGGTAATGAGAGTAATTT+GGG + chr3.1:82444721-82444740 MS.gene32806:CDS 30.0%
! GAATTTTTGTGTTCATATGC+AGG + chr3.1:82444078-82444097 MS.gene32806:intron 30.0%
! TTCAACAACATCAAAGGTTT+TGG - chr3.1:82443654-82443673 None:intergenic 30.0%
!! AAAATGATTCTGGAGCTATA+AGG + chr3.1:82444545-82444564 MS.gene32806:CDS 30.0%
!! AAATGATTCTGGAGCTATAA+GGG + chr3.1:82444546-82444565 MS.gene32806:CDS 30.0%
!! TCGTAATAGCTTTGTTGTTT+GGG + chr3.1:82443712-82443731 MS.gene32806:CDS 30.0%
AATTATCTGCTATGGAAGCT+AGG + chr3.1:82444359-82444378 MS.gene32806:CDS 35.0%
CAAAACCCATTTGATCAACA+AGG - chr3.1:82444894-82444913 None:intergenic 35.0%
CAGAACCTTGTTGATCAAAT+GGG + chr3.1:82444886-82444905 MS.gene32806:CDS 35.0%
GCACATAATAGAGAGTTGAA+AGG + chr3.1:82444466-82444485 MS.gene32806:CDS 35.0%
GTGGTGCTTGTATTGAATTA+GGG + chr3.1:82444233-82444252 MS.gene32806:CDS 35.0%
TAACTCTTCATCTTCCTCTT+CGG + chr3.1:82443565-82443584 MS.gene32806:CDS 35.0%
TAATCAACCACAGACTCAAT+TGG - chr3.1:82444577-82444596 None:intergenic 35.0%
TAATTCAATACAAGCACCAC+CGG - chr3.1:82444233-82444252 None:intergenic 35.0%
TGAATCAAGCAGTGAAGTTA+AGG + chr3.1:82444672-82444691 MS.gene32806:CDS 35.0%
TGGATCTTCAACAACATCAA+AGG - chr3.1:82443660-82443679 None:intergenic 35.0%
TGGTGCTTGTATTGAATTAG+GGG + chr3.1:82444234-82444253 MS.gene32806:CDS 35.0%
TGTATTGAATTAGGGGAGTA+TGG + chr3.1:82444241-82444260 MS.gene32806:CDS 35.0%
TTAGCATATGTCAGTGTTGT+TGG + chr3.1:82443530-82443549 MS.gene32806:CDS 35.0%
! TTACTGTGTTTGAAGTAACG+AGG - chr3.1:82443767-82443786 None:intergenic 35.0%
!! CTCGTAATAGCTTTGTTGTT+TGG + chr3.1:82443711-82443730 MS.gene32806:CDS 35.0%
!! TCAAAGGTTTTGGTGAGAAA+TGG - chr3.1:82443644-82443663 None:intergenic 35.0%
!!! GGGTTTTAGAAAAGTTGATC+CGG + chr3.1:82444099-82444118 MS.gene32806:intron 35.0%
AACTCTTCATCTTCCTCTTC+GGG + chr3.1:82443566-82443585 MS.gene32806:CDS 40.0%
AAGGGAGCAATTATCTGCTA+TGG + chr3.1:82444351-82444370 MS.gene32806:CDS 40.0%
AATGGAAGGATTGCACGAAA+TGG + chr3.1:82443610-82443629 MS.gene32806:CDS 40.0%
ATGGAAGGATTGCACGAAAT+GGG + chr3.1:82443611-82443630 MS.gene32806:CDS 40.0%
ATTTCGTGCAATCCTTCCAT+TGG - chr3.1:82443611-82443630 None:intergenic 40.0%
CCAGACAATGTTGATGTATC+CGG + chr3.1:82443993-82444012 MS.gene32806:intron 40.0%
CCATGAAACAATGGAATCAG+TGG - chr3.1:82443684-82443703 None:intergenic 40.0%
CTTACACCAAAACCAATGGA+AGG + chr3.1:82443596-82443615 MS.gene32806:CDS 40.0%
GAAACAATGGAATCAGTGGA+TGG - chr3.1:82443680-82443699 None:intergenic 40.0%
GAAGGGAATTAGAGTGAAAG+AGG + chr3.1:82443499-82443518 MS.gene32806:CDS 40.0%
GACTACTATTGAGTCTGAGA+TGG + chr3.1:82444624-82444643 MS.gene32806:CDS 40.0%
GATTCCATTGTTTCATGGAG+CGG + chr3.1:82443686-82443705 MS.gene32806:CDS 40.0%
GCAGAACCTTGTTGATCAAA+TGG + chr3.1:82444885-82444904 MS.gene32806:CDS 40.0%
GCGTTGAAATGAAGCGAAAA+CGG + chr3.1:82444488-82444507 MS.gene32806:CDS 40.0%
GGATTTACTGAACCAAGAGT+TGG + chr3.1:82444759-82444778 MS.gene32806:CDS 40.0%
GGTGGTGCTTGTATTGAATT+AGG + chr3.1:82444232-82444251 MS.gene32806:CDS 40.0%
GTAACGAGGTAAAATAGCAG+TGG - chr3.1:82443753-82443772 None:intergenic 40.0%
TAATTTGGGTGATGCTGTGT+GGG + chr3.1:82444735-82444754 MS.gene32806:CDS 40.0%
TCTTGTCAAGAACAGCAAGA+GGG + chr3.1:82444598-82444617 MS.gene32806:CDS 40.0%
TGAGATTGAGAGGTTGAGAA+GGG + chr3.1:82444273-82444292 MS.gene32806:CDS 40.0%
TGGCTGAGATTGTGAAGTTA+AGG + chr3.1:82444311-82444330 MS.gene32806:CDS 40.0%
TGTAAGATTTGAACCCGAAG+AGG - chr3.1:82443582-82443601 None:intergenic 40.0%
TTGAAATGAAGCGAAAACGG+AGG + chr3.1:82444491-82444510 MS.gene32806:CDS 40.0%
! AAAAGTTGATCCGGATCGAT+GGG + chr3.1:82444108-82444127 MS.gene32806:CDS 40.0%
! CTTTTCTACCAAATCCTCCA+CGG - chr3.1:82444842-82444861 None:intergenic 40.0%
! TTACTGAACCAAGAGTTGGT+TGG + chr3.1:82444763-82444782 MS.gene32806:CDS 40.0%
! TTTTCTACCAAATCCTCCAC+GGG - chr3.1:82444841-82444860 None:intergenic 40.0%
!! AAGGTTTTGGTGAGAAATGG+TGG - chr3.1:82443641-82443660 None:intergenic 40.0%
!! CCACTGATTCCATTGTTTCA+TGG + chr3.1:82443681-82443700 MS.gene32806:CDS 40.0%
!!! TGCAATCCTTCCATTGGTTT+TGG - chr3.1:82443605-82443624 None:intergenic 40.0%
CCAATCACAACTTCATCCTC+TGG - chr3.1:82444796-82444815 None:intergenic 45.0%
CCAGAGGATGAAGTTGTGAT+TGG + chr3.1:82444793-82444812 MS.gene32806:CDS 45.0%
CCGGATACATCAACATTGTC+TGG - chr3.1:82443996-82444015 None:intergenic 45.0%
CTCTTGTCAAGAACAGCAAG+AGG + chr3.1:82444597-82444616 MS.gene32806:CDS 45.0%
GCAGCAGCAGCATAATTCAA+GGG + chr3.1:82444333-82444352 MS.gene32806:CDS 45.0%
GCATTGAGTTCAGTTCCAAG+TGG + chr3.1:82444703-82444722 MS.gene32806:CDS 45.0%
GTAATTTGGGTGATGCTGTG+TGG + chr3.1:82444734-82444753 MS.gene32806:CDS 45.0%
GTCTTGAAGGTGAGATTGAG+AGG + chr3.1:82444263-82444282 MS.gene32806:CDS 45.0%
GTGAGATTGAGAGGTTGAGA+AGG + chr3.1:82444272-82444291 MS.gene32806:CDS 45.0%
TTCTCAAATTGATGTGCCCG+TGG + chr3.1:82444822-82444841 MS.gene32806:CDS 45.0%
! GAAAAGTTGATCCGGATCGA+TGG + chr3.1:82444107-82444126 MS.gene32806:CDS 45.0%
! TGAACCAAGAGTTGGTTGGT+GGG + chr3.1:82444767-82444786 MS.gene32806:CDS 45.0%
! TTAGGGGAGTATGGTCTTGA+AGG + chr3.1:82444250-82444269 MS.gene32806:CDS 45.0%
!! AAGGGACAGGGCTGTTTTAA+TGG + chr3.1:82444291-82444310 MS.gene32806:CDS 45.0%
!! GTTTTGGTGAGAAATGGTGG+TGG - chr3.1:82443638-82443657 None:intergenic 45.0%
!!! CGAACGAAGGGTTTTTAGCT+GGG + chr3.1:82444137-82444156 MS.gene32806:CDS 45.0%
AGCTCCGCTCCATGAAACAA+TGG - chr3.1:82443693-82443712 None:intergenic 50.0%
CAAGCTATGCAGCAAGAGAC+CGG + chr3.1:82444211-82444230 MS.gene32806:CDS 50.0%
GATGGGAGTTTGCGAACGAA+GGG + chr3.1:82444125-82444144 MS.gene32806:CDS 50.0%
GATTCCCACCAACCAACTCT+TGG - chr3.1:82444774-82444793 None:intergenic 50.0%
GGCAGCAGCAGCATAATTCA+AGG + chr3.1:82444332-82444351 MS.gene32806:CDS 50.0%
TCAAATTGATGTGCCCGTGG+AGG + chr3.1:82444825-82444844 MS.gene32806:CDS 50.0%
TGAGAGGTTGAGAAGGGACA+GGG + chr3.1:82444279-82444298 MS.gene32806:CDS 50.0%
TTCGCAAACTCCCATCGATC+CGG - chr3.1:82444121-82444140 None:intergenic 50.0%
TTGAGAGGTTGAGAAGGGAC+AGG + chr3.1:82444278-82444297 MS.gene32806:CDS 50.0%
! CTGAACCAAGAGTTGGTTGG+TGG + chr3.1:82444766-82444785 MS.gene32806:CDS 50.0%
! GCTCACTGCTAGTTCGAGTT+TGG + chr3.1:82444513-82444532 MS.gene32806:CDS 50.0%
!!! AAGGGTTTTTAGCTGGGCAG+AGG + chr3.1:82444143-82444162 MS.gene32806:CDS 50.0%
!!! GCGAACGAAGGGTTTTTAGC+TGG + chr3.1:82444136-82444155 MS.gene32806:CDS 50.0%
CGATGGGAGTTTGCGAACGA+AGG + chr3.1:82444124-82444143 MS.gene32806:CDS 55.0%
GGCTGTGCCAATTGAGTCTG+TGG + chr3.1:82444567-82444586 MS.gene32806:CDS 55.0%
TGATGTGCCCGTGGAGGATT+TGG + chr3.1:82444831-82444850 MS.gene32806:CDS 55.0%
TTTGGGTGATGCTGTGTGGG+AGG + chr3.1:82444738-82444757 MS.gene32806:CDS 55.0%
!! GTTGGTTGGTGGGAATCCAG+AGG + chr3.1:82444777-82444796 MS.gene32806:CDS 55.0%
GCTATGCAGCAAGAGACCGG+TGG + chr3.1:82444214-82444233 MS.gene32806:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 82443497 82444921 82443497 ID=MS.gene32806
chr3.1 mRNA 82443497 82444921 82443497 ID=MS.gene32806.t1;Parent=MS.gene32806
chr3.1 exon 82443497 82443820 82443497 ID=MS.gene32806.t1.exon1;Parent=MS.gene32806.t1
chr3.1 CDS 82443497 82443820 82443497 ID=cds.MS.gene32806.t1;Parent=MS.gene32806.t1
chr3.1 exon 82444100 82444921 82444100 ID=MS.gene32806.t1.exon2;Parent=MS.gene32806.t1
chr3.1 CDS 82444100 82444921 82444100 ID=cds.MS.gene32806.t1;Parent=MS.gene32806.t1
Gene Sequence

>MS.gene32806

ATGAAGGGAATTAGAGTGAAAGAGGAAGAAACATTAGCATATGTCAGTGTTGTTGGTTCAACATCATGTAACTCTTCATCTTCCTCTTCGGGTTCAAATCTTACACCAAAACCAATGGAAGGATTGCACGAAATGGGTCCACCACCATTTCTCACCAAAACCTTTGATGTTGTTGAAGATCCATCCACTGATTCCATTGTTTCATGGAGCGGAGCTCGTAATAGCTTTGTTGTTTGGGATCTTCACAAATTCTCCACTGCTATTTTACCTCGTTACTTCAAACACAGTAATTTCTCTAGCTTCGTTCGACAACTCAATACCTATGTAAGTTAACTATTACTTTTTGATTTTATTTCATTGTTGTTAAAATTAGTTCAATATTGTTCAACTTAGATACGAGTTAGTTATAATTAGTTGAAAATTTGTTATTATTATTAGTTAGCTGATTTAGACCGACACATATGATTACATTCAATTATTTTGATGTATGTCAGTGCCAGACAATGTTGATGTATCCGGTGTTTGTGAATTTGTTCTTGCTTATCAAACTTAAGATGTTTTTTATGTTCAATTTGTTGAGCGAATTTTTGTGTTCATATGCAGGGTTTTAGAAAAGTTGATCCGGATCGATGGGAGTTTGCGAACGAAGGGTTTTTAGCTGGGCAGAGGAACTTGTTGAAAACAATCAAGAGAAGAAGAAATGTGACACAATCACAAGCTATGCAGCAAGAGACCGGTGGTGCTTGTATTGAATTAGGGGAGTATGGTCTTGAAGGTGAGATTGAGAGGTTGAGAAGGGACAGGGCTGTTTTAATGGCTGAGATTGTGAAGTTAAGGCAGCAGCAGCATAATTCAAGGGAGCAATTATCTGCTATGGAAGCTAGGTTGTTGATTACTGAGAAGAAACATCAACAGATGATGAATTTTCTTGCAAAAGCACTTAGCAATCAATCTTTTATACAACAATTGGCACATAATAGAGAGTTGAAAGGCGTTGAAATGAAGCGAAAACGGAGGCTCACTGCTAGTTCGAGTTTGGAGAATTTGCAAAATGATTCTGGAGCTATAAGGGCTGTGCCAATTGAGTCTGTGGTTGATTACTCTTGTCAAGAACAGCAAGAGGGTTTGACTACTATTGAGTCTGAGATGGAGACACTGTTAAGTGCTTATGATAATGAATCAAGCAGTGAAGTTAAGGACTACACTGCATTGAGTTCAGTTCCAAGTGGTAATGAGAGTAATTTGGGTGATGCTGTGTGGGAGGATTTACTGAACCAAGAGTTGGTTGGTGGGAATCCAGAGGATGAAGTTGTGATTGGTGATTTTTCTCAAATTGATGTGCCCGTGGAGGATTTGGTAGAAAAGAATGATAATTGGACTGTGGATTTGCAGAACCTTGTTGATCAAATGGGTTTTGAACCTTAG
Protein sequence

>MS.gene32806.t1

MKGIRVKEEETLAYVSVVGSTSCNSSSSSSGSNLTPKPMEGLHEMGPPPFLTKTFDVVEDPSTDSIVSWSGARNSFVVWDLHKFSTAILPRYFKHSNFSSFVRQLNTYGFRKVDPDRWEFANEGFLAGQRNLLKTIKRRRNVTQSQAMQQETGGACIELGEYGLEGEIERLRRDRAVLMAEIVKLRQQQHNSREQLSAMEARLLITEKKHQQMMNFLAKALSNQSFIQQLAHNRELKGVEMKRKRRLTASSSLENLQNDSGAIRAVPIESVVDYSCQEQQEGLTTIESEMETLLSAYDNESSSEVKDYTALSSVPSGNESNLGDAVWEDLLNQELVGGNPEDEVVIGDFSQIDVPVEDLVEKNDNWTVDLQNLVDQMGFEP