Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024731.t1 | XP_003610916.1 | 99.1 | 113 | 1 | 0 | 1 | 113 | 1 | 113 | 7.90E-59 | 236.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024731.t1 | Q9T0G2 | 91.1 | 112 | 10 | 0 | 1 | 112 | 1 | 112 | 3.7e-57 | 221.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024731.t1 | G7K7W9 | 99.1 | 113 | 1 | 0 | 1 | 113 | 1 | 113 | 5.7e-59 | 236.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene024731 | MS.gene55480 | PPI |
| MS.gene074110 | MS.gene024731 | PPI |
| MS.gene024731 | MS.gene074111 | PPI |
| MS.gene024731 | MS.gene47121 | PPI |
| MS.gene024731 | MS.gene55486 | PPI |
| MS.gene47123 | MS.gene024731 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024731.t1 | MTR_5g008460 | 99.115 | 113 | 1 | 0 | 1 | 113 | 1 | 113 | 7.94e-82 | 234 |
| MS.gene024731.t1 | MTR_7g080803 | 89.381 | 113 | 12 | 0 | 1 | 113 | 1 | 113 | 8.10e-75 | 217 |
| MS.gene024731.t1 | MTR_7g080793 | 70.370 | 108 | 31 | 1 | 1 | 108 | 1 | 107 | 4.43e-52 | 159 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024731.t1 | AT4G10040 | 91.071 | 112 | 10 | 0 | 1 | 112 | 1 | 112 | 1.79e-75 | 219 |
| MS.gene024731.t1 | AT1G22840 | 88.393 | 112 | 13 | 0 | 1 | 112 | 1 | 112 | 8.81e-73 | 212 |
| MS.gene024731.t1 | AT1G22840 | 84.524 | 84 | 13 | 0 | 1 | 84 | 1 | 84 | 2.06e-50 | 155 |
Find 41 sgRNAs with CRISPR-Local
Find 95 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCAATCTGAATGGTTTGTT+TGG | 0.305862 | 5.3:+2759358 | MS.gene024731:CDS |
| AATAAGATCAGCACGTTCTT+GGG | 0.344346 | 5.3:-2760067 | None:intergenic |
| CAATAAGATCAGCACGTTCT+TGG | 0.348957 | 5.3:-2760068 | None:intergenic |
| ACAAGAACATGGCTGTGAAT+TGG | 0.349587 | 5.3:+2759425 | MS.gene024731:CDS |
| TTATTTGCTTAACCCCAAAA+AGG | 0.352975 | 5.3:+2759468 | MS.gene024731:CDS |
| CAAGAACATGGCTGTGAATT+GGG | 0.354427 | 5.3:+2759426 | MS.gene024731:CDS |
| AGCTGCCGAGTAGGAATATC+CGG | 0.375428 | 5.3:-2759401 | None:intergenic |
| GGTTTGTTTGGAAGGCAATC+AGG | 0.375977 | 5.3:+2759370 | MS.gene024731:CDS |
| GCTCTTGTGCAGTATATTCC+AGG | 0.394249 | 5.3:+2760012 | MS.gene024731:intron |
| GGTGTGACACTGAGCGCATT+TGG | 0.415295 | 5.3:-2758285 | None:intergenic |
| TTCTTGGGGTTTCTTTAGAC+CGG | 0.417289 | 5.3:-2760052 | None:intergenic |
| ATCTCCGGATTTGGCATCAC+CGG | 0.423980 | 5.3:-2758249 | None:intergenic |
| TCCAAACAAACCATTCAGAT+TGG | 0.424075 | 5.3:-2759359 | None:intergenic |
| ATCTGAATGGTTTGTTTGGA+AGG | 0.441498 | 5.3:+2759362 | MS.gene024731:CDS |
| TCAGTGTCACACCGTCGATA+AGG | 0.441684 | 5.3:+2758295 | MS.gene024731:CDS |
| CAGTGTCACACCGTCGATAA+GGG | 0.454494 | 5.3:+2758296 | MS.gene024731:CDS |
| GGGACCAAGATGGTATTTCC+CGG | 0.471291 | 5.3:+2760033 | MS.gene024731:CDS |
| CCTCCCGGTGATGCCAAATC+CGG | 0.475381 | 5.3:+2758245 | MS.gene024731:CDS |
| CCAAACAAACCATTCAGATT+GGG | 0.484079 | 5.3:-2759358 | None:intergenic |
| GATCTAACCTTGTTTGTGAC+CGG | 0.503624 | 5.3:-2758321 | None:intergenic |
| AGGCAATCAGGCACTACTCC+CGG | 0.508362 | 5.3:+2759382 | MS.gene024731:CDS |
| GGAAATACCATCTTGGTCCC+TGG | 0.530773 | 5.3:-2760030 | None:intergenic |
| TAGACCGGGAAATACCATCT+TGG | 0.536477 | 5.3:-2760037 | None:intergenic |
| ATCTCAGGACCCAATCTGAA+TGG | 0.539851 | 5.3:+2759349 | MS.gene024731:intron |
| TCTCCGGATTTGGCATCACC+GGG | 0.561033 | 5.3:-2758248 | None:intergenic |
| CTCTTGTGCAGTATATTCCA+GGG | 0.564315 | 5.3:+2760013 | MS.gene024731:intron |
| TCGTTTGAACAAGCACCTCC+CGG | 0.572771 | 5.3:+2758230 | MS.gene024731:CDS |
| GTTCTTGTTAGCTGCCGAGT+AGG | 0.576933 | 5.3:-2759410 | None:intergenic |
| TCTTGGGGTTTCTTTAGACC+GGG | 0.586837 | 5.3:-2760051 | None:intergenic |
| AAGGGTGCCGGTCACAAACA+AGG | 0.600344 | 5.3:+2758314 | MS.gene024731:CDS |
| CTCGGCAGCTAACAAGAACA+TGG | 0.601333 | 5.3:+2759414 | MS.gene024731:CDS |
| ATAAGATCAGCACGTTCTTG+GGG | 0.603518 | 5.3:-2760066 | None:intergenic |
| TGATCTTATTGCATATCTGA+AGG | 0.616592 | 5.3:+2760080 | MS.gene024731:CDS |
| CACACCGTCGATAAGGGTGC+CGG | 0.624319 | 5.3:+2758302 | MS.gene024731:CDS |
| TACTCCCGGATATTCCTACT+CGG | 0.628766 | 5.3:+2759396 | MS.gene024731:CDS |
| AATATATAATCACTGTGCAG+TGG | 0.632456 | 5.3:-2760106 | None:intergenic |
| GTGACCGGCACCCTTATCGA+CGG | 0.634194 | 5.3:-2758306 | None:intergenic |
| GCTGCCGAGTAGGAATATCC+GGG | 0.639197 | 5.3:-2759400 | None:intergenic |
| GTATATTCCAGGGACCAAGA+TGG | 0.661039 | 5.3:+2760023 | MS.gene024731:intron |
| GAACATGGCTGTGAATTGGG+AGG | 0.673762 | 5.3:+2759429 | MS.gene024731:CDS |
| CCGGATTTGGCATCACCGGG+AGG | 0.684893 | 5.3:-2758245 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATACTAATATAATATCAA+GGG | - | chr5.3:2759172-2759191 | None:intergenic | 10.0% |
| !!! | AATAATATTTAGTATTGATT+AGG | - | chr5.3:2759805-2759824 | None:intergenic | 10.0% |
| !! | AATCTCTATATCTATAATTT+AGG | + | chr5.3:2759231-2759250 | MS.gene024731:intron | 15.0% |
| !! | ATCATCTAATATTATTGTTT+AGG | + | chr5.3:2758645-2758664 | MS.gene024731:intron | 15.0% |
| !! | CATATACTAATATAATATCA+AGG | - | chr5.3:2759173-2759192 | None:intergenic | 15.0% |
| !! | TATTTCAATATTTGATTCAT+AGG | + | chr5.3:2759081-2759100 | MS.gene024731:intron | 15.0% |
| !!! | AAATAATTACATTTTCAATG+AGG | - | chr5.3:2758562-2758581 | None:intergenic | 15.0% |
| !! | ATAAAAAAGCTATTGTATGT+AGG | + | chr5.3:2758716-2758735 | MS.gene024731:intron | 20.0% |
| !! | ATTTGTGTTTATTTCATTGT+TGG | + | chr5.3:2758469-2758488 | MS.gene024731:intron | 20.0% |
| !! | GATTTATGATTCAAGAAATT+TGG | + | chr5.3:2758614-2758633 | MS.gene024731:intron | 20.0% |
| !! | GTGAAAATAATGTATAATCA+TGG | - | chr5.3:2758419-2758438 | None:intergenic | 20.0% |
| !! | TAAAAAAGCTATTGTATGTA+GGG | + | chr5.3:2758717-2758736 | MS.gene024731:intron | 20.0% |
| !! | TAGTACATAATATTGTGAAA+TGG | - | chr5.3:2758937-2758956 | None:intergenic | 20.0% |
| !! | TATTTGATTCATAGGTTTAT+TGG | + | chr5.3:2759089-2759108 | MS.gene024731:intron | 20.0% |
| !! | TTAGGATAATATTTGATTGA+TGG | + | chr5.3:2758663-2758682 | MS.gene024731:intron | 20.0% |
| !!! | AATATAATATCAAGGGTATA+AGG | - | chr5.3:2759165-2759184 | None:intergenic | 20.0% |
| !!! | TTGTTTTTCTTTTCGTTTAT+AGG | + | chr5.3:2759839-2759858 | MS.gene024731:intron | 20.0% |
| ! | AATAAGATTTACAGCTTAGT+TGG | + | chr5.3:2759544-2759563 | MS.gene024731:intron | 25.0% |
| ! | ATTAGCATATTACCACAATA+TGG | - | chr5.3:2759934-2759953 | None:intergenic | 25.0% |
| ! | GAATAACGTGATTATCTATT+TGG | + | chr5.3:2759302-2759321 | MS.gene024731:intron | 25.0% |
| !! | AGATTGTATTGTACCTTTTT+GGG | - | chr5.3:2759484-2759503 | None:intergenic | 25.0% |
| !! | TAGATTGTATTGTACCTTTT+TGG | - | chr5.3:2759485-2759504 | None:intergenic | 25.0% |
| !!! | TTTCTAAGATTTCCATATTG+TGG | + | chr5.3:2759869-2759888 | MS.gene024731:intron | 25.0% |
| AAACGAAAAGAAAAACAAGC+AGG | - | chr5.3:2759837-2759856 | None:intergenic | 30.0% | |
| AACGAAAAGAAAAACAAGCA+GGG | - | chr5.3:2759836-2759855 | None:intergenic | 30.0% | |
| CATAGATCAGTAACAAATTG+TGG | - | chr5.3:2759989-2760008 | None:intergenic | 30.0% | |
| TGATCTTATTGCATATCTGA+AGG | + | chr5.3:2760080-2760099 | MS.gene024731:CDS | 30.0% | |
| TGGTAATATGCTACGTTTAT+AGG | + | chr5.3:2759889-2759908 | MS.gene024731:intron | 30.0% | |
| TTAGATCAGTTTCTCATATG+TGG | + | chr5.3:2758337-2758356 | MS.gene024731:intron | 30.0% | |
| TTATTTGCTTAACCCCAAAA+AGG | + | chr5.3:2759468-2759487 | MS.gene024731:CDS | 30.0% | |
| ! | AAAGATTTTATCTCCGGATT+TGG | - | chr5.3:2758261-2758280 | None:intergenic | 30.0% |
| ! | GATTGTATTGTACCTTTTTG+GGG | - | chr5.3:2759483-2759502 | None:intergenic | 30.0% |
| !! | TAGGTTTATTGGTGTTGTAA+TGG | + | chr5.3:2759100-2759119 | MS.gene024731:intron | 30.0% |
| AACACAAATTGCATAGCATG+TGG | - | chr5.3:2758458-2758477 | None:intergenic | 35.0% | |
| AATAAGATCAGCACGTTCTT+GGG | - | chr5.3:2760070-2760089 | None:intergenic | 35.0% | |
| AATTGCATAGCATGTGGAAA+TGG | - | chr5.3:2758452-2758471 | None:intergenic | 35.0% | |
| ACCTTCACATATAACAAGCA+AGG | + | chr5.3:2759691-2759710 | MS.gene024731:intron | 35.0% | |
| AGATCAGTAACAAATTGTGG+TGG | - | chr5.3:2759986-2760005 | None:intergenic | 35.0% | |
| AGTTGTAAGCTAATCCAAAC+TGG | + | chr5.3:2759765-2759784 | MS.gene024731:intron | 35.0% | |
| ATGTGGAAATGGAACGATTA+AGG | - | chr5.3:2758441-2758460 | None:intergenic | 35.0% | |
| CCAAACAAACCATTCAGATT+GGG | - | chr5.3:2759361-2759380 | None:intergenic | 35.0% | |
| CGTAGCATATTACCACAATA+TGG | - | chr5.3:2759884-2759903 | None:intergenic | 35.0% | |
| TCCAAACAAACCATTCAGAT+TGG | - | chr5.3:2759362-2759381 | None:intergenic | 35.0% | |
| TTGATTAGGAGTGAAGCATA+TGG | - | chr5.3:2759791-2759810 | None:intergenic | 35.0% | |
| ! | GGTTCGAAAGATTTTATCTC+CGG | - | chr5.3:2758267-2758286 | None:intergenic | 35.0% |
| ! | GTTTTTCATTACTCCTTTGC+TGG | + | chr5.3:2759192-2759211 | MS.gene024731:intron | 35.0% |
| !! | AGCACAATAAGTTTGTTGCA+CGG | - | chr5.3:2758501-2758520 | None:intergenic | 35.0% |
| !! | ATCTGAATGGTTTGTTTGGA+AGG | + | chr5.3:2759362-2759381 | MS.gene024731:CDS | 35.0% |
| !! | TTGCTTGTTATATGTGAAGG+TGG | - | chr5.3:2759692-2759711 | None:intergenic | 35.0% |
| !! | TTTGCCTTGTTGATAATCTC+AGG | + | chr5.3:2759334-2759353 | MS.gene024731:intron | 35.0% |
| !!! | TGTTTTGACATAGTGCTGTA+GGG | + | chr5.3:2758988-2759007 | MS.gene024731:intron | 35.0% |
| ACAAGAACATGGCTGTGAAT+TGG | + | chr5.3:2759425-2759444 | MS.gene024731:CDS | 40.0% | |
| AGCAGTAGTATTGTCAATCG+TGG | + | chr5.3:2758848-2758867 | MS.gene024731:intron | 40.0% | |
| ATAAGATCAGCACGTTCTTG+GGG | - | chr5.3:2760069-2760088 | None:intergenic | 40.0% | |
| CAAGAACATGGCTGTGAATT+GGG | + | chr5.3:2759426-2759445 | MS.gene024731:CDS | 40.0% | |
| CAATAAGATCAGCACGTTCT+TGG | - | chr5.3:2760071-2760090 | None:intergenic | 40.0% | |
| CTCTTGTGCAGTATATTCCA+GGG | + | chr5.3:2760013-2760032 | MS.gene024731:intron | 40.0% | |
| GAAAAGAAAAACAAGCAGGG+TGG | - | chr5.3:2759833-2759852 | None:intergenic | 40.0% | |
| GCAGTAGTATTGTCAATCGT+GGG | + | chr5.3:2758849-2758868 | MS.gene024731:intron | 40.0% | |
| GTTATATGTGAAGGTGGCAA+TGG | - | chr5.3:2759686-2759705 | None:intergenic | 40.0% | |
| TTCTTGGGGTTTCTTTAGAC+CGG | - | chr5.3:2760055-2760074 | None:intergenic | 40.0% | |
| ! | GATCTAACCTTGTTTGTGAC+CGG | - | chr5.3:2758324-2758343 | None:intergenic | 40.0% |
| !! | CCCAATCTGAATGGTTTGTT+TGG | + | chr5.3:2759358-2759377 | MS.gene024731:CDS | 40.0% |
| !! | GCCTTGCTTGTTATATGTGA+AGG | - | chr5.3:2759695-2759714 | None:intergenic | 40.0% |
| !!! | CTGTTTTGACATAGTGCTGT+AGG | + | chr5.3:2758987-2759006 | MS.gene024731:intron | 40.0% |
| AGTGAAGCATATGGCCAGTT+TGG | - | chr5.3:2759782-2759801 | None:intergenic | 45.0% | |
| ATCAAACTCAGTGCCAGCAA+AGG | - | chr5.3:2759208-2759227 | None:intergenic | 45.0% | |
| ATCTCAGGACCCAATCTGAA+TGG | + | chr5.3:2759349-2759368 | MS.gene024731:intron | 45.0% | |
| GCTCTTGTGCAGTATATTCC+AGG | + | chr5.3:2760012-2760031 | MS.gene024731:intron | 45.0% | |
| GGGTCCTGAGATTATCAACA+AGG | - | chr5.3:2759341-2759360 | None:intergenic | 45.0% | |
| GTATATTCCAGGGACCAAGA+TGG | + | chr5.3:2760023-2760042 | MS.gene024731:intron | 45.0% | |
| TACTCCCGGATATTCCTACT+CGG | + | chr5.3:2759396-2759415 | MS.gene024731:CDS | 45.0% | |
| TAGACCGGGAAATACCATCT+TGG | - | chr5.3:2760040-2760059 | None:intergenic | 45.0% | |
| TCTTGGGGTTTCTTTAGACC+GGG | - | chr5.3:2760054-2760073 | None:intergenic | 45.0% | |
| ! | GGTTTGTTTGGAAGGCAATC+AGG | + | chr5.3:2759370-2759389 | MS.gene024731:CDS | 45.0% |
| ! | GTATTGTCAATCGTGGGTTG+CGG | + | chr5.3:2758855-2758874 | MS.gene024731:intron | 45.0% |
| AGCTGCCGAGTAGGAATATC+CGG | - | chr5.3:2759404-2759423 | None:intergenic | 50.0% | |
| ATCTCCGGATTTGGCATCAC+CGG | - | chr5.3:2758252-2758271 | None:intergenic | 50.0% | |
| CAGTGTCACACCGTCGATAA+GGG | + | chr5.3:2758296-2758315 | MS.gene024731:CDS | 50.0% | |
| CTCGGCAGCTAACAAGAACA+TGG | + | chr5.3:2759414-2759433 | MS.gene024731:CDS | 50.0% | |
| GAACATGGCTGTGAATTGGG+AGG | + | chr5.3:2759429-2759448 | MS.gene024731:CDS | 50.0% | |
| GGAAATACCATCTTGGTCCC+TGG | - | chr5.3:2760033-2760052 | None:intergenic | 50.0% | |
| GGGACCAAGATGGTATTTCC+CGG | + | chr5.3:2760033-2760052 | MS.gene024731:CDS | 50.0% | |
| GTTCTTGTTAGCTGCCGAGT+AGG | - | chr5.3:2759413-2759432 | None:intergenic | 50.0% | |
| TCAGTGTCACACCGTCGATA+AGG | + | chr5.3:2758295-2758314 | MS.gene024731:CDS | 50.0% | |
| TCGTTTGAACAAGCACCTCC+CGG | + | chr5.3:2758230-2758249 | MS.gene024731:CDS | 50.0% | |
| AGGCAATCAGGCACTACTCC+CGG | + | chr5.3:2759382-2759401 | MS.gene024731:CDS | 55.0% | |
| GCTGCCGAGTAGGAATATCC+GGG | - | chr5.3:2759403-2759422 | None:intergenic | 55.0% | |
| GGTGTGACACTGAGCGCATT+TGG | - | chr5.3:2758288-2758307 | None:intergenic | 55.0% | |
| TCTCCGGATTTGGCATCACC+GGG | - | chr5.3:2758251-2758270 | None:intergenic | 55.0% | |
| !! | AAGGGTGCCGGTCACAAACA+AGG | + | chr5.3:2758314-2758333 | MS.gene024731:CDS | 55.0% |
| CCTCCCGGTGATGCCAAATC+CGG | + | chr5.3:2758245-2758264 | MS.gene024731:CDS | 60.0% | |
| GTGACCGGCACCCTTATCGA+CGG | - | chr5.3:2758309-2758328 | None:intergenic | 60.0% | |
| ! | CACACCGTCGATAAGGGTGC+CGG | + | chr5.3:2758302-2758321 | MS.gene024731:CDS | 60.0% |
| CCGGATTTGGCATCACCGGG+AGG | - | chr5.3:2758248-2758267 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 2758224 | 2760119 | 2758224 | ID=MS.gene024731 |
| chr5.3 | mRNA | 2758224 | 2760119 | 2758224 | ID=MS.gene024731.t1;Parent=MS.gene024731 |
| chr5.3 | exon | 2758224 | 2758335 | 2758224 | ID=MS.gene024731.t1.exon1;Parent=MS.gene024731.t1 |
| chr5.3 | CDS | 2758224 | 2758335 | 2758224 | ID=cds.MS.gene024731.t1;Parent=MS.gene024731.t1 |
| chr5.3 | exon | 2759356 | 2759489 | 2759356 | ID=MS.gene024731.t1.exon2;Parent=MS.gene024731.t1 |
| chr5.3 | CDS | 2759356 | 2759489 | 2759356 | ID=cds.MS.gene024731.t1;Parent=MS.gene024731.t1 |
| chr5.3 | exon | 2760024 | 2760119 | 2760024 | ID=MS.gene024731.t1.exon3;Parent=MS.gene024731.t1 |
| chr5.3 | CDS | 2760024 | 2760119 | 2760024 | ID=cds.MS.gene024731.t1;Parent=MS.gene024731.t1 |
| Gene Sequence |
| Protein sequence |
