AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025221

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025221.t1 MTR_2g041640 97.819 871 15 3 1 868 1 870 0.0 1757
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025221.t1 AT1G77550 67.828 861 264 5 10 868 3 852 0.0 1204

Find 216 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCCACTTTCCAATATTT+TGG 0.092480 2.2:+46121134 None:intergenic
CATATTATATCTGCATCTTT+TGG 0.139160 2.2:+46118362 None:intergenic
TATTCCTGTCAGATTGTTTC+TGG 0.155637 2.2:-46117646 MS.gene025221:CDS
CAATTTCGGGGAGTGGGCTT+TGG 0.216553 2.2:-46121875 MS.gene025221:CDS
CAACTTTCACAAGCAATTTC+GGG 0.220240 2.2:-46121888 MS.gene025221:CDS
GGAATTCTACTCGCCGCTTC+AGG 0.227443 2.2:-46123871 MS.gene025221:CDS
CACATGATGGGCGTCCTTTA+CGG 0.233516 2.2:-46119937 MS.gene025221:CDS
TCAACTTTCACAAGCAATTT+CGG 0.237496 2.2:-46121889 MS.gene025221:CDS
ACTCATCTGTCTCAACTTAT+TGG 0.250244 2.2:-46117902 MS.gene025221:CDS
CCAAACATTTCTGAACTAAA+TGG 0.271808 2.2:-46121168 MS.gene025221:CDS
GGGAAGTGCCATTGAAATTC+TGG 0.272207 2.2:-46121199 MS.gene025221:CDS
AGTTTCCATGAGTCGGATTA+TGG 0.278974 2.2:+46117775 None:intergenic
TGCACTCGTGACTTTCTTCT+TGG 0.281535 2.2:-46120212 MS.gene025221:CDS
CTGTATCTCTTGCAGTTAAA+TGG 0.290895 2.2:-46117198 MS.gene025221:intron
TTAGTGGCACTTAGCCTATA+TGG 0.294517 2.2:-46122163 MS.gene025221:CDS
AGGTACGTGATGGATGAATT+TGG 0.296880 2.2:-46120679 MS.gene025221:CDS
GTCGATATTCCTGGTCCTCT+TGG 0.300317 2.2:-46121222 MS.gene025221:CDS
CAGGTTGTTCAATATACTTC+TGG 0.310081 2.2:+46117738 None:intergenic
TATTTGTGCATCCAAAATAT+TGG 0.310689 2.2:-46121145 MS.gene025221:CDS
ACTTCTTGAATAATCAATTC+AGG 0.312500 2.2:+46122124 None:intergenic
CAAAAGATGCAGATATAATA+TGG 0.314062 2.2:-46118361 MS.gene025221:CDS
AGCTAACAACCAATATTCTT+TGG 0.314596 2.2:-46117529 MS.gene025221:CDS
CGTGACTTTCTTCTTGGTAT+TGG 0.317812 2.2:-46120206 MS.gene025221:CDS
TGAAACTCATTTCACTGTTA+TGG 0.319368 2.2:-46117484 MS.gene025221:intron
AATAGTGCGGGCCATGTTCC+AGG 0.320213 2.2:+46117826 None:intergenic
GTTGATGATGATGATGTTTC+GGG 0.325483 2.2:-46122986 MS.gene025221:CDS
AATTTCGGGGAGTGGGCTTT+GGG 0.325758 2.2:-46121874 MS.gene025221:CDS
ACTTACCAATTCTGAAGAAC+TGG 0.326545 2.2:+46122058 None:intergenic
TGATGATATGTTTCTCTCAT+TGG 0.333223 2.2:-46122858 MS.gene025221:CDS
ACTTGGATAAAATAGTTCTC+TGG 0.333720 2.2:+46120137 None:intergenic
ATTCTCTTGCTTACGTTCAT+TGG 0.335166 2.2:-46121371 MS.gene025221:CDS
AATAAATCACAAGAATATTA+AGG 0.336524 2.2:-46117349 MS.gene025221:CDS
TCTAGTGATGGCGATCTTGC+AGG 0.343730 2.2:-46121952 MS.gene025221:intron
TCGATATTCCTGGTCCTCTT+GGG 0.347686 2.2:-46121221 MS.gene025221:CDS
TATGGCCAACACGGAATGTT+TGG 0.355349 2.2:-46120247 MS.gene025221:CDS
TCTTGCAGGTATTATTCTTA+AGG 0.357157 2.2:-46121938 MS.gene025221:CDS
TCTTAAGGAGTTACCAGAAC+TGG 0.362100 2.2:-46121923 MS.gene025221:CDS
ACATGATGGGCGTCCTTTAC+GGG 0.362567 2.2:-46119936 MS.gene025221:CDS
ATAACCCAGTTCTTCAGAAT+TGG 0.365410 2.2:-46122063 MS.gene025221:intron
AAGTTATACATCTAATTTCC+AGG 0.366200 2.2:+46116607 None:intergenic
AGACGGCGGTGGAGGGATTC+CGG 0.366662 2.2:+46123844 None:intergenic
TTGTAAGCTTGTTTCCATAT+AGG 0.366689 2.2:+46122149 None:intergenic
GGCTCATCAGGAGTCCATTC+AGG 0.370563 2.2:+46121078 None:intergenic
GTTGAAGAAATCACAACCTT+TGG 0.377369 2.2:+46116759 None:intergenic
ACTCGCCGCTTCAGGGTTAC+CGG 0.378225 2.2:-46123863 MS.gene025221:CDS
CTCGCCGCCGTCGAAGGTTT+CGG 0.378738 2.2:+46123804 None:intergenic
CTTTCCCTTTCAGAATTTAG+TGG 0.379886 2.2:-46122179 MS.gene025221:intron
AAAGGTGCCACTCTAAAGTT+TGG 0.383885 2.2:+46120631 None:intergenic
TGAACAACCTGCTTTATTCC+AGG 0.384791 2.2:-46117726 MS.gene025221:CDS
GATGTGTGTGGATATTGATT+TGG 0.393550 2.2:-46123032 MS.gene025221:CDS
TTGCAAGCTCGTGTACAATC+AGG 0.394641 2.2:+46116820 None:intergenic
GAAGACTTCGCCAAAGTTCA+CGG 0.397878 2.2:-46123892 MS.gene025221:CDS
GGACTTGCCTGCTAGATTCC+CGG 0.398457 2.2:-46122837 MS.gene025221:intron
TGTTGATGATGATGATGTTT+CGG 0.400062 2.2:-46122987 MS.gene025221:CDS
TTCTTCCTCGCCTTCCTGAA+TGG 0.401426 2.2:-46121092 MS.gene025221:CDS
CATTAATCAATTTCCATTTG+AGG 0.403628 2.2:-46118279 MS.gene025221:CDS
GAATTATAAATCTCCAGTTC+TGG 0.404024 2.2:+46121910 None:intergenic
GAGAAGGACTAGACAACCTT+TGG 0.406467 2.2:-46117859 MS.gene025221:CDS
CTTTCGTACTTGAACATTCG+TGG 0.406787 2.2:-46121445 MS.gene025221:CDS
CGGTCATGAATAGGCTCATC+AGG 0.408012 2.2:+46121066 None:intergenic
CCAACATCAAGGGCAATTTA+CGG 0.409401 2.2:-46117094 MS.gene025221:CDS
CCATTTAGTTCAGAAATGTT+TGG 0.413238 2.2:+46121168 None:intergenic
GGTTCGGAGCATGCACCCTT+TGG 0.416642 2.2:-46117672 MS.gene025221:CDS
GCCACATTGCACTTATAATA+CGG 0.416942 2.2:+46121046 None:intergenic
CTCTCAGAGGACTGCACAGT+GGG 0.420225 2.2:+46119974 None:intergenic
GGTTAGTGTTGTAGAAATGT+TGG 0.423832 2.2:-46122957 MS.gene025221:CDS
ATAATCCGACTCATGGAAAC+TGG 0.425940 2.2:-46117773 MS.gene025221:CDS
CTTATAATACGGTCATGAAT+AGG 0.427782 2.2:+46121057 None:intergenic
GAATTCTACTCGCCGCTTCA+GGG 0.429428 2.2:-46123870 MS.gene025221:CDS
ATGAATTTGGTTCTGCTTTG+CGG 0.431191 2.2:-46120666 MS.gene025221:CDS
CTCTTGCTTACGTTCATTGG+AGG 0.433275 2.2:-46121368 MS.gene025221:intron
TTGAGATGGTTGGAGCTTGA+TGG 0.434714 2.2:-46122893 MS.gene025221:CDS
CGAAAGGGATGGCAAACAAA+TGG 0.435231 2.2:+46121462 None:intergenic
CTTGGATAAAATAGTTCTCT+GGG 0.435795 2.2:+46120138 None:intergenic
TCTTCCTCGTTGATCATGCT+TGG 0.437053 2.2:-46123699 MS.gene025221:CDS
AAGAGATTCCAGAATTTCAA+TGG 0.443802 2.2:+46121191 None:intergenic
GTAACTGAGCTTTGCTCCAA+TGG 0.447485 2.2:+46121418 None:intergenic
CACAAGCAATTTCGGGGAGT+GGG 0.449915 2.2:-46121881 MS.gene025221:CDS
GCTGCGTGAAGTTCCGGGAT+TGG 0.452297 2.2:-46123074 MS.gene025221:CDS
TCTCTTCGCAAGGTTCGATC+TGG 0.454357 2.2:+46123776 None:intergenic
TGATCTCCGTTATGTAGTAC+TGG 0.455881 2.2:-46117693 MS.gene025221:CDS
AGGTTTCGGTGGAGAGTTTG+TGG 0.460242 2.2:+46123818 None:intergenic
GATGTTGGACTATGCATCTC+AGG 0.462658 2.2:+46117109 None:intergenic
TTTAGCAGAAACAATTCAAA+AGG 0.464036 2.2:-46118234 MS.gene025221:intron
TTACCAACCTCTAGTAACTT+AGG 0.465893 2.2:+46117040 None:intergenic
ATGACAAGACAAGCCTCAAA+TGG 0.465912 2.2:+46118266 None:intergenic
ACTCTCCGACGCTTACAAAC+AGG 0.466139 2.2:-46123668 MS.gene025221:intron
ATTCCTGTCAGATTGTTTCT+GGG 0.469381 2.2:-46117645 MS.gene025221:intron
CCCTCTGGCATGAAAAGAAA+AGG 0.470377 2.2:+46120613 None:intergenic
TCCGTTATGTAGTACTGGTT+CGG 0.471117 2.2:-46117688 MS.gene025221:CDS
GTTCGAGAGGTGAAAGAGAA+AGG 0.471606 2.2:-46122926 MS.gene025221:CDS
TTTATCTTGCTTCAGGCACA+TGG 0.472517 2.2:-46117962 MS.gene025221:intron
TGTGGAAGAGACGGCGGTGG+AGG 0.472903 2.2:+46123836 None:intergenic
CACAGTGGGTGGTATTAAAC+TGG 0.473738 2.2:+46119988 None:intergenic
TCCGGGATTGGCAGAGAGGA+TGG 0.477944 2.2:-46123062 MS.gene025221:CDS
ATTGAACCAAGCAGTAAGTC+TGG 0.478752 2.2:+46120163 None:intergenic
ATCTCTTGCAGTTAAATGGT+TGG 0.478877 2.2:-46117194 MS.gene025221:intron
TCACAAGCAATTTCGGGGAG+TGG 0.481347 2.2:-46121882 MS.gene025221:CDS
CCCATCCTCTCTGCCAATCC+CGG 0.482424 2.2:+46123061 None:intergenic
TATGACATGGATACCGTTGT+TGG 0.485794 2.2:-46116796 MS.gene025221:CDS
TGGATATACATGCAAGAGTA+AGG 0.489710 2.2:-46117174 MS.gene025221:CDS
TATAATATGGACCTGCGTAC+AGG 0.490503 2.2:-46118348 MS.gene025221:CDS
CAACCTCTAGTAACTTAGGT+TGG 0.493695 2.2:+46117044 None:intergenic
CCGTAAATTGCCCTTGATGT+TGG 0.495083 2.2:+46117094 None:intergenic
TATGATTCGATCGGTGTTTG+AGG 0.499225 2.2:-46117149 MS.gene025221:CDS
CGATATTCCTGGTCCTCTTG+GGG 0.503309 2.2:-46121220 MS.gene025221:CDS
GAAATGCTCGCCGCCGTCGA+AGG 0.504521 2.2:+46123798 None:intergenic
GAACATTCGTGGAAATCCAT+TGG 0.506538 2.2:-46121434 MS.gene025221:CDS
GAGTAGAATTCCGTGAACTT+TGG 0.507996 2.2:+46123882 None:intergenic
AGAAGTATTCGCCCACATGA+TGG 0.518087 2.2:-46119950 MS.gene025221:CDS
GGACTGTCAGCTTGATCAGA+TGG 0.518311 2.2:+46121820 None:intergenic
AGGCACATGGATCTCCTCAA+TGG 0.518425 2.2:-46117949 MS.gene025221:CDS
TTGGACCAGTTTCCATGAGT+CGG 0.519542 2.2:+46117768 None:intergenic
TCCATGAGTCGGATTATGGC+TGG 0.520674 2.2:+46117779 None:intergenic
TGATGATGTTTCGGGAGAGA+AGG 0.520808 2.2:-46122978 MS.gene025221:CDS
ATCAAATTTAAGCATCTCAA+AGG 0.523069 2.2:-46122100 MS.gene025221:CDS
CTCATGCGATTCTCTTCGCA+AGG 0.523435 2.2:+46123766 None:intergenic
GAAAGGGATGGCAAACAAAT+GGG 0.527047 2.2:+46121463 None:intergenic
TGAGTTTCAAGATTATAAGT+AGG 0.529055 2.2:+46117920 None:intergenic
CCTTTCCAAACATTCCGTGT+TGG 0.533360 2.2:+46120242 None:intergenic
TACAGTAGTATCAATAGTGC+GGG 0.533708 2.2:+46117814 None:intergenic
GCCGATGCTAGATTGCCCTC+TGG 0.535309 2.2:+46120598 None:intergenic
TTAAGCATCTCAAAGGACTC+TGG 0.537705 2.2:-46122093 MS.gene025221:CDS
ACCTTTGGATCTTAAAACCC+TGG 0.538572 2.2:-46117844 MS.gene025221:CDS
GAAGTATTCGCCCACATGAT+GGG 0.539104 2.2:-46119949 MS.gene025221:CDS
TGTAGAAATGTTGGAGAGTG+AGG 0.539327 2.2:-46122948 MS.gene025221:CDS
AGTTAGACGAAACCTCTGTA+TGG 0.539543 2.2:-46120987 MS.gene025221:intron
CACGAATGTTCAAGTACGAA+AGG 0.541790 2.2:+46121446 None:intergenic
GAACAACCTGCTTTATTCCA+GGG 0.542789 2.2:-46117725 MS.gene025221:CDS
TTACTGACCGGGAATCTAGC+AGG 0.542860 2.2:+46122830 None:intergenic
GGATAATTCTAGGTACGTGA+TGG 0.545152 2.2:-46120689 MS.gene025221:intron
AGAAGAAGAAATGGTAGCAA+TGG 0.546649 2.2:-46123944 None:intergenic
TGCAAGCTCGTGTACAATCA+GGG 0.549178 2.2:+46116821 None:intergenic
GCATCCAAAATATTGGAAAG+TGG 0.551382 2.2:-46121138 MS.gene025221:CDS
GTTTGTGGAAGAGACGGCGG+TGG 0.553090 2.2:+46123833 None:intergenic
CGTAAAGGACGCCCATCATG+TGG 0.553316 2.2:+46119938 None:intergenic
GTTGGAGAGTGAGGTTCGAG+AGG 0.553772 2.2:-46122939 MS.gene025221:CDS
ATGGATACCGTTGTTGGAGA+AGG 0.554146 2.2:-46116790 MS.gene025221:CDS
ATATCTGTGTATACCCGTAA+AGG 0.555996 2.2:+46119923 None:intergenic
AACTTTGGCGAAGTCTTCGA+AGG 0.557163 2.2:+46123897 None:intergenic
CCGGGATTGGCAGAGAGGAT+GGG 0.560887 2.2:-46123061 MS.gene025221:CDS
GTTCTGCCAGACTTACTGCT+TGG 0.561878 2.2:-46120169 MS.gene025221:CDS
GATCTTAAAACCCTGGAACA+TGG 0.564811 2.2:-46117837 MS.gene025221:CDS
CTTACCAATTCTGAAGAACT+GGG 0.565554 2.2:+46122059 None:intergenic
ATCGGTGTTTGAGGCAGCTG+CGG 0.566183 2.2:-46117140 MS.gene025221:CDS
TGACGGTACATTGAGATGGT+TGG 0.567506 2.2:-46122903 MS.gene025221:CDS
TCCACCGAAACCTTCGACGG+CGG 0.568274 2.2:-46123808 MS.gene025221:CDS
TTGGACCTGTTTGTAAGCGT+CGG 0.569827 2.2:+46123663 None:intergenic
ACCAGCCATAATCCGACTCA+TGG 0.571309 2.2:-46117780 MS.gene025221:CDS
ATACCCAGAAACAATCTGAC+AGG 0.581860 2.2:+46117642 None:intergenic
AGATGCATAGTCCAACATCA+AGG 0.583143 2.2:-46117105 MS.gene025221:CDS
ACGAATGTTCAAGTACGAAA+GGG 0.584367 2.2:+46121447 None:intergenic
GTGGAAGAGACGGCGGTGGA+GGG 0.585364 2.2:+46123837 None:intergenic
GGTTGATGAGGAGTTGAAGA+AGG 0.587491 2.2:-46118327 MS.gene025221:CDS
AACAACCTGCTTTATTCCAG+GGG 0.587507 2.2:-46117724 MS.gene025221:CDS
TTTCCAACCTAAGTTACTAG+AGG 0.588700 2.2:-46117047 MS.gene025221:intron
GAGGAGTTGAAGAAGGCCAC+GGG 0.591379 2.2:-46118320 MS.gene025221:CDS
TTGCGCAGAGATATATGGAA+AGG 0.594711 2.2:-46121848 MS.gene025221:CDS
GCCGCCGTCGAAGGTTTCGG+TGG 0.597756 2.2:+46123807 None:intergenic
GCCAGAGGGCAATCTAGCAT+CGG 0.598031 2.2:-46120599 MS.gene025221:CDS
TGAGGAGTTGAAGAAGGCCA+CGG 0.599213 2.2:-46118321 MS.gene025221:CDS
GGAGAAGGAGGTATTGCCAA+AGG 0.601959 2.2:-46116775 MS.gene025221:CDS
AATACCTCCTTCTCCAACAA+CGG 0.603511 2.2:+46116783 None:intergenic
TTGCTGATCTGTTATTCCCG+TGG 0.606174 2.2:+46118304 None:intergenic
CACTTACTGATTGCAAACTC+AGG 0.606354 2.2:+46119872 None:intergenic
AACTTTCACAAGCAATTTCG+GGG 0.606957 2.2:-46121887 MS.gene025221:CDS
TTTCTATTCTATGGCCAACA+CGG 0.607111 2.2:-46120256 MS.gene025221:CDS
GACTGTCAGCTTGATCAGAT+GGG 0.607915 2.2:+46121821 None:intergenic
ATAGTGCGGGCCATGTTCCA+GGG 0.608612 2.2:+46117827 None:intergenic
CTGACAGGAATATCTCCAAA+GGG 0.611128 2.2:+46117657 None:intergenic
TCTGACAGGAATATCTCCAA+AGG 0.611646 2.2:+46117656 None:intergenic
GGAGTCCATTCAGGAAGGCG+AGG 0.613302 2.2:+46121087 None:intergenic
TGGAGAGTTTGTGGAAGAGA+CGG 0.613450 2.2:+46123827 None:intergenic
GATAGTGAAACTGAGGTGAA+TGG 0.613747 2.2:-46123010 MS.gene025221:CDS
ATGATCAACGAGGAAGACAT+TGG 0.615083 2.2:+46123705 None:intergenic
AGTAAGGACTATGATTCGAT+CGG 0.623174 2.2:-46117158 MS.gene025221:CDS
GCTCTCAGAGGACTGCACAG+TGG 0.626388 2.2:+46119973 None:intergenic
CATTCACAATCTGATCAACA+AGG 0.628020 2.2:-46121758 MS.gene025221:intron
GATGGGTTCTTTGATGTGTG+TGG 0.629001 2.2:-46123044 MS.gene025221:CDS
GATACCGTTGTTGGAGAAGG+AGG 0.629194 2.2:-46116787 MS.gene025221:CDS
GATGCATAGTCCAACATCAA+GGG 0.630418 2.2:-46117104 MS.gene025221:CDS
TATTATGTACGCAAAAGAGA+AGG 0.632002 2.2:-46117875 MS.gene025221:CDS
CTCCTCATCAACCTGTACGC+AGG 0.634177 2.2:+46118337 None:intergenic
TCCGAACCAGTACTACATAA+CGG 0.635574 2.2:+46117687 None:intergenic
CCAACACGGAATGTTTGGAA+AGG 0.635916 2.2:-46120242 MS.gene025221:CDS
ATGTTCAAGTACGAAAGGGA+TGG 0.635997 2.2:+46121451 None:intergenic
GCATGTTCTCAGAATTATCG+TGG 0.636330 2.2:-46117375 MS.gene025221:intron
TCAGAGGACTGCACAGTGGG+TGG 0.639769 2.2:+46119977 None:intergenic
AAGGTGACGGTACATTGAGA+TGG 0.641533 2.2:-46122907 MS.gene025221:CDS
GTCAAGTACTCTTCCACGTG+AGG 0.644264 2.2:+46119899 None:intergenic
AAGTTCCGGGATTGGCAGAG+AGG 0.644963 2.2:-46123066 MS.gene025221:CDS
AGTGGGTGGTATTAAACTGG+TGG 0.645039 2.2:+46119991 None:intergenic
GACCTGCGTACAGGTTGATG+AGG 0.645215 2.2:-46118339 MS.gene025221:CDS
GGATTCCGGTAACCCTGAAG+CGG 0.645492 2.2:+46123858 None:intergenic
AGATTAGATACACTCAGCAG+TGG 0.646587 2.2:+46121799 None:intergenic
TCTCAGCAGCTCTAACAAGT+CGG 0.647266 2.2:+46121396 None:intergenic
TTACAGTAGTATCAATAGTG+CGG 0.648290 2.2:+46117813 None:intergenic
GCAATCTAGCATCGGCTGTG+AGG 0.649411 2.2:-46120591 MS.gene025221:intron
GAATTTGAGGAAGAACATCA+AGG 0.650482 2.2:-46117315 MS.gene025221:intron
ACGAGCTTGCAAATATGACA+TGG 0.651494 2.2:-46116809 MS.gene025221:CDS
CATCAGGAGTCCATTCAGGA+AGG 0.654679 2.2:+46121082 None:intergenic
CTCTCCACCGAAACCTTCGA+CGG 0.658578 2.2:-46123811 MS.gene025221:CDS
GATATTCCTGGTCCTCTTGG+GGG 0.661353 2.2:-46121219 MS.gene025221:CDS
GAGGTGAAAGAGAAAGGTGA+CGG 0.664017 2.2:-46122920 MS.gene025221:CDS
TGATTTGGATAGTGAAACTG+AGG 0.668818 2.2:-46123017 MS.gene025221:CDS
TAAGTAGGCTGCAACCATTG+AGG 0.676253 2.2:+46117935 None:intergenic
TGATGAGCCAAACTTTAGAG+TGG 0.680214 2.2:-46120638 MS.gene025221:CDS
ATACACAGATATACCTCACG+TGG 0.685643 2.2:-46119912 MS.gene025221:CDS
GTAAAGGACGCCCATCATGT+GGG 0.688444 2.2:+46119939 None:intergenic
GCGAATACTTCTGCTCTCAG+AGG 0.689727 2.2:+46119961 None:intergenic
ATGAATGTCATACCATACAG+AGG 0.704504 2.2:+46120975 None:intergenic
TTGGATAAAATAGTTCTCTG+GGG 0.706460 2.2:+46120139 None:intergenic
AGAGTTTGTGGAAGAGACGG+CGG 0.716886 2.2:+46123830 None:intergenic
TAGAATCCGAAGTCAAAACG+AGG 0.732532 2.2:+46123740 None:intergenic
ACCGTATTATAAGTGCAATG+TGG 0.733685 2.2:-46121047 MS.gene025221:CDS
AAGTCCAAGCATGATCAACG+AGG 0.739980 2.2:+46123695 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr2.2 gene 46116618 46123956 46116618 ID=MS.gene025221
chr2.2 mRNA 46116618 46123956 46116618 ID=MS.gene025221.t1;Parent=MS.gene025221
chr2.2 exon 46123669 46123956 46123669 ID=MS.gene025221.t1.exon1;Parent=MS.gene025221.t1
chr2.2 CDS 46123669 46123956 46123669 ID=cds.MS.gene025221.t1;Parent=MS.gene025221.t1
chr2.2 exon 46122838 46123095 46122838 ID=MS.gene025221.t1.exon2;Parent=MS.gene025221.t1
chr2.2 CDS 46122838 46123095 46122838 ID=cds.MS.gene025221.t1;Parent=MS.gene025221.t1
chr2.2 exon 46122064 46122188 46122064 ID=MS.gene025221.t1.exon3;Parent=MS.gene025221.t1
chr2.2 CDS 46122064 46122188 46122064 ID=cds.MS.gene025221.t1;Parent=MS.gene025221.t1
chr2.2 exon 46121759 46121969 46121759 ID=MS.gene025221.t1.exon4;Parent=MS.gene025221.t1
chr2.2 CDS 46121759 46121969 46121759 ID=cds.MS.gene025221.t1;Parent=MS.gene025221.t1
chr2.2 exon 46121369 46121494 46121369 ID=MS.gene025221.t1.exon5;Parent=MS.gene025221.t1
chr2.2 CDS 46121369 46121494 46121369 ID=cds.MS.gene025221.t1;Parent=MS.gene025221.t1
chr2.2 exon 46120988 46121244 46120988 ID=MS.gene025221.t1.exon6;Parent=MS.gene025221.t1
chr2.2 CDS 46120988 46121244 46120988 ID=cds.MS.gene025221.t1;Parent=MS.gene025221.t1
chr2.2 exon 46120592 46120699 46120592 ID=MS.gene025221.t1.exon7;Parent=MS.gene025221.t1
chr2.2 CDS 46120592 46120699 46120592 ID=cds.MS.gene025221.t1;Parent=MS.gene025221.t1
chr2.2 exon 46120139 46120280 46120139 ID=MS.gene025221.t1.exon8;Parent=MS.gene025221.t1
chr2.2 CDS 46120139 46120280 46120139 ID=cds.MS.gene025221.t1;Parent=MS.gene025221.t1
chr2.2 exon 46119879 46120044 46119879 ID=MS.gene025221.t1.exon9;Parent=MS.gene025221.t1
chr2.2 CDS 46119879 46120044 46119879 ID=cds.MS.gene025221.t1;Parent=MS.gene025221.t1
chr2.2 exon 46118235 46118392 46118235 ID=MS.gene025221.t1.exon10;Parent=MS.gene025221.t1
chr2.2 CDS 46118235 46118392 46118235 ID=cds.MS.gene025221.t1;Parent=MS.gene025221.t1
chr2.2 exon 46117646 46117969 46117646 ID=MS.gene025221.t1.exon11;Parent=MS.gene025221.t1
chr2.2 CDS 46117646 46117969 46117646 ID=cds.MS.gene025221.t1;Parent=MS.gene025221.t1
chr2.2 exon 46117485 46117559 46117485 ID=MS.gene025221.t1.exon12;Parent=MS.gene025221.t1
chr2.2 CDS 46117485 46117559 46117485 ID=cds.MS.gene025221.t1;Parent=MS.gene025221.t1
chr2.2 exon 46117316 46117385 46117316 ID=MS.gene025221.t1.exon13;Parent=MS.gene025221.t1
chr2.2 CDS 46117316 46117385 46117316 ID=cds.MS.gene025221.t1;Parent=MS.gene025221.t1
chr2.2 exon 46117048 46117205 46117048 ID=MS.gene025221.t1.exon14;Parent=MS.gene025221.t1
chr2.2 CDS 46117048 46117205 46117048 ID=cds.MS.gene025221.t1;Parent=MS.gene025221.t1
chr2.2 exon 46116720 46116854 46116720 ID=MS.gene025221.t1.exon15;Parent=MS.gene025221.t1
chr2.2 CDS 46116720 46116854 46116720 ID=cds.MS.gene025221.t1;Parent=MS.gene025221.t1
chr2.2 exon 46116618 46116644 46116618 ID=MS.gene025221.t1.exon16;Parent=MS.gene025221.t1
chr2.2 CDS 46116618 46116644 46116618 ID=cds.MS.gene025221.t1;Parent=MS.gene025221.t1
Gene Sequence

>MS.gene025221

ATGGTAGCAATGGCAACAACAACAAAGAGAATCGAAACCTTCGAAGACTTCGCCAAAGTTCACGGAATTCTACTCGCCGCTTCAGGGTTACCGGAATCCCTCCACCGCCGTCTCTTCCACAAACTCTCCACCGAAACCTTCGACGGCGGCGAGCATTTCCAGATCGAACCTTGCGAAGAGAATCGCATGAGACGCCTCGTTTTGACTTCGGATTCTATGGCTAAAGACTCCAATGTCTTCCTCGTTGATCATGCTTGGACTTTCAGACTCTCCGACGCTTACAAACAGGTCCAAACCTTCTTCACATTCTTCATGCAACACTAGTTTTACATAACTAATGAAAATAATTTGTAGCTTATACGAAAAAAGTTTAACATTATTTTATCTTTTTGTACATAAATGACTTGTACATAAGTGTTTATCATGATAATAATTAATAAGCACTTGGGCTATAAGTTGCAAATTAAGTTGTTTATCCAAACATAGCTTAAATTAATTGTAGGTGGTTAAATATGAGGACCAAATTGATTGATAGTTAGTTGTTAAACTATAGGGAGCTAATAGATAGTAAGTGGTTAGCTATATTATAGGACCAACTTGATAGTTTAATAGAGTAAGTGATCATTTTGAGATATTCATAATCTCAAAGTGTCTTAGAGTGTTAGGGACTAATTTGGTTGTTTACTCACTAATTTTTTTATTTTTAGGGTTTAATTTTTGCTCACTAACATTGTGGCGTAATTTTATCCATGTAGCCGACCCAACTTACTGGGATAAGGCTTAGTTGTAGTAGTAGGGTTTCATTTTTGCGTTAATTTTTTTGATTTTTTTGTTGTTTGTTGTTCTTTTTGGTGATTTTAGCTGCGTGAAGTTCCGGGATTGGCAGAGAGGATGGGTTCTTTGATGTGTGTGGATATTGATTTGGATAGTGAAACTGAGGTGAATGGTGTTGATGATGATGATGTTTCGGGAGAGAAGGTTAGTGTTGTAGAAATGTTGGAGAGTGAGGTTCGAGAGGTGAAAGAGAAAGGTGACGGTACATTGAGATGGTTGGAGCTTGATGGTCTTGATATCGATGATGATATGTTTCTCTCATTGGACTTGCCTGCTAGATTCCCGGTCAGTAACTCTTAATCTTGTTTTTTTTATTTTGTTCATATTTTATTTTATAGTTATAATTTCTATAGGTTCTATTGTTTTGAAAGTGTGACTTTGTTCATTCTGCAAATAGAAATCATGCTCATCAAACTTAGGAATGTTATATTATGATATGAATTGAATTTGAGTGGCAAGATGGCAAACATTATTACTTCCTTCATCCGTTATTATAAGACTCACTTTTGACTTTTGTTAAAAAAATTAAGACTTTTGATATTTCCTTGTTCTTTAATATTGGACTCTTTTCAAAATTTTACATTCAATAATAATTACTTTACAAAAATTTCCTTATTTAATTTACAATTGTGAAACGTAAATTTGTTTTTTCACTCTTACAACATCAATAATTGTGAGGATATTTATAGGAAGTAACACAAATTTGACAGGTTTATTGTTATGGCTATTTTTCTTAATACCGAGTAAATTTTGTAAGCGAGTCTCATAATAAGAGACGATGACAGTATTATTTAATTATCGTCGGAGTATTCAATTTTCCTGTCATCTCAGATCCCGTTATTCTAGATGCTGCAAGCCAATTTTAGTTTCACGTTTCTTGTTATAATTATTTTAATTTGAATTCTGATGAACTATCCTCCCCTTTCCCTTTCAGAATTTAGTGGCACTTAGCCTATATGGAAACAAGCTTACAAGACCTGAATTGATTATTCAAGAAGTTATCAAATTTAAGCATCTCAAAGGACTCTGGCTGAACAATAACCCAGTTCTTCAGAATTGGTAAGTTTTCTCATATTTTAAAAATTTCTTAAACCACATATTCCTTTTTGGTAAGTCCTTGTTGTTAAGGAGAGCTAAATTACTTTACTTCTAGTGATGGCGATCTTGCAGGTATTATTCTTAAGGAGTTACCAGAACTGGAGATTTATAATTCAACTTTCACAAGCAATTTCGGGGAGTGGGCTTTGGGCTTTTGCGCAGAGATATATGGAAAGGATAACCCATCTGATCAAGCTGACAGTCCACTGCTGAGTGTATCTAATCTTGATCTTTCAAATAGAAACATTCACAATCTGATCAACAAGGTTGAACCTTTTGTTATGCTTATTTTCTATCTTATTGGCTTATGTTTCAAAAGTATAGATAGACTTAAGCATATTATTGTACTGAGTTCAAAAATCTGATAATTATGAATCTCTCCTTGAGTCTATGCTCAAGAGATACAAAATGGTAAACAAAGCATAATCACTAAAAAGATTTTAAACACATGGCATTGCCAATTCATTTTTTCCAATCCCATATATGTATGTTTTGATCGAATTATTTTGACGATTCCCTCATTTGGTTAGGACTTCTCACCCATTTGTTTGCCATCCCTTTCGTACTTGAACATTCGTGGAAATCCATTGGAGCAAAGCTCAGTTACCGACTTGTTAGAGCTGCTGAGACGATTCTCTTGCTTACGTTCATTGGAGGTACTTTGATTTATGTTATGACAATTTTTCAAGCCCCAAATTGCCTTTGTTATTTATTTAATTTTGTATGTCCATTCTCTGGTAACCAGTGTTTTGGCCTTTGGGAATTTGAGCAAATTTTTAGGTCGATATTCCTGGTCCTCTTGGGGGAAGTGCCATTGAAATTCTGGAATCTCTTCCAAACATTTCTGAACTAAATGGTATTTGTGCATCCAAAATATTGGAAAGTGGAAAGCATGTTATTGATTCAGTGCTTCTTCCTCGCCTTCCTGAATGGACTCCTGATGAGCCTATTCATGACCGTATTATAAGTGCAATGTGGCAATATCTAATGACATATAGACTTGCTGATGAAGAAAAGTTAGACGAAACCTCTGTATGGTATGACATTCATTTTACTTGTTTTACTTTCTTGGTTCTAATGTAGCACTAGGTTTCTATCAATCAGTAAATGCTTTTAAGTTTTGTGTTTTTGCACTTCTTGTTGAAATACACAATTTCATCAAGGTTGTCTGATTTGTAAATTATTGTTGTGACCCTACTCGAATCAATGGGTCAATGTTTAACCATTCAAATTGTTGGGTCAGTACATTATATGAAACATATGATCAATTTGGAATTTCCCAACAATAAAGAAAACTAACATCCCATTGTTTTGGATAATTCTAGGTACGTGATGGATGAATTTGGTTCTGCTTTGCGGCACAGTGATGAGCCAAACTTTAGAGTGGCACCTTTTCTTTTCATGCCAGAGGGCAATCTAGCATCGGCTGTGAGGTTTTCTTCTATATTTTACTTTTATATTTTGCTTTTCTACTATATTTTACTTTCTGCTTTACTATATGATTGAGCTTGCATGATTTGTAAAAAAATATTATATAAAAAAATTTGATTATAGGTTTTGCTCTGTAATTCTTGCTCATTAATATATAATAACTTAATTACCTGTGTAGCTCATATTAATGACTTCTAACTGCGAAACTTCTCCTCCATCCTTTTTATGAATTACATGTATTTTATGGCATGCCCTCTGGATTTGTGATAATTCTAAATTAGTCTCTGTAAGTAATAACTCCCACTTTTCACAGCTTTTCTATTCTATGGCCAACACGGAATGTTTGGAAAGGCGATGAATGCACTCGTGACTTTCTTCTTGGTATTGGAGAAGATAAACAACGTTCTGCCAGACTTACTGCTTGGTTCAATACCCCAGAGAACTATTTTATCCAAGTAATTCTTCTATTACTAAGGCAGATTATGATTCATATCTGTTCCACACGAAAGTTTTAGGGAAGTTTGAGGCTAATTATTTTATTATTTTCAGGAGTATGAGAAGTACAATCAGAGATTGCAATCCACCAGTTTAATACCACCCACTGTGCAGTCCTCTGAGAGCAGAAGTATTCGCCCACATGATGGGCGTCCTTTACGGGTATACACAGATATACCTCACGTGGAAGAGTACTTGACACATCCTGAGTTTGCAATCAGTAAGTGGACTCTATGCTTCTGATAGACACTGGATTAAATTATAGAGATTAACAGAATGAAAATAATGAAATTTTATCACATATCCTCCTCCAACTTTTGTTCCTTTTTGTTAGGTTGCTTTGAAAAAGGCACAAGCAGGGAATTATTCGTCCCTGAGTTTATTGATAACCGAAAGGTTTATTGATAACAGAAAGAAAAGGGTTACTTTCCTCACTGGAATAAGCCAATTTCTTAATAAAGTTCAGAATGCCTAATCTAGGGGCCGGAGAAGCTATTTATAGATTCCCCGGAATAAGCCAGTCAATTTGTTAATAAAGTTCAGAATGCCTAATCTAGGGTTACAGCCTATGTGACTGGCAGTTGCCAATTAAGACGGTTACAGCCTGTCAAGGCAGTTACAAGGGAAATGCTCTAGTGAGTCACAGCTTATGGTGATCATTCTGAATTCTGGTCACCTACTACCTATATTTGGAGTCAATCCTTTGATATGTCTAACACTTTTCTTGATCTTAAAATCTGTTTTCTTGAACATCACCTCACCTCTCAGAAGCTTTCCCAGGTTTCCTTAGGATTGTTAACCTCCCACTACTTGTACCAATGTTGGAATAGTAGTGCTAGCAACACTCTCTTTTGAGCACTCTCTCTAATACTCACTCTCTTATTGGTTTAAATCATTGTGGGTCTATACTTTGGAAATATAATGGCGAGACATACATTGATTCCATCCAATAAAAGAGTGAGTGCTAGAGAGAGTGTTCAAAAGTGAGTGTTGCTAGCATTCCTCATGTCGGAATGTTCACCCAGTGCTTCCACGCCCATCATTTTCTGTGTCGAAGCGCTCCTCTGCAAATCTTCTTGAATTTACTTCTTGATCAGTCTACACAAAGTTCCCCATTTCTTGGGAGCTTCAGTACTAGTTGATAGGTACTGGTTCAATATATGGAGGCATACAAATGGAAAATGAGGAAGTTTTATAATAATTCCTCCTAACTTAACTCTGGATGAATGGTTATCCAGGTCAAGATGAGCAGGATGACATATAATGATGTCTTCCCCAGGTATTTGAGCTGGTCTCTCCTAAGTCCCACTCAAAACTAATTAACTTCCTCTAGCTTCTTTGCCTCTATTAAACAGCCTAACTAATTAACCACTCTAACAACTTTCCTAACAGAGACTGTGCTTGGCTAGCAGGGTTCCTTGACCTAACTGATGTAAGCTTTCCTAATTGGGTCTGCTCCTAACAACATGCTTGAACTCTTTTATCAACTCATAATATACAGTTAAACAATCATGTAACTTGAAAAGTGAAGAGCCTAAATTGAATTATCCTTGTTTCATCTGAACCTTTTCTCATTCTGAGAAGCATTGACATCTTATGTTTTTCATAAAATTCCCATGGATATTTGATTCAATGATTACCTCCACGTTTTTAAAGTTTGCTGGATTCTGACCTTCTCCCTCTCTTTTGAATGTGTTTGGCTACAGTAAAGGACCCAAAAGATGCAGATATAATATGGACCTGCGTACAGGTTGATGAGGAGTTGAAGAAGGCCACGGGAATAACAGATCAGCAATACATTAATCAATTTCCATTTGAGGCTTGTCTTGTCATGAAGCATCATTTAGCAGAAACAATTCAAAAGGTGATATTATAGTTAATCAACATTGTATGTTGATGCTGATGAGATTATCCCAAATGTCTGACCTGTTTTCATTATGCATTTCTAGTGAAATTCCATCATACATTCAGAGAAATATGTATTATATTATATATTATATATTCATCAGAGCATGCTACATTTAATTTTATTTTCAAGTCACTTACAAATGAATTGAAGTGTTGGTTTCTCTTTTGAGGCTTTTTAAATAATGAATCATTTTGGGGTTGGGTGTTTTATCTTGCTTCAGGCACATGGATCTCCTCAATGGTTGCAGCCTACTTATAATCTTGAAACTCATCTGTCTCAACTTATTGGTGACTATTATGTACGCAAAAGAGAAGGACTAGACAACCTTTGGATCTTAAAACCCTGGAACATGGCCCGCACTATTGATACTACTGTAACTGATAACTTACCAGCCATAATCCGACTCATGGAAACTGGTCCAAAAATATGCCAGAAGTATATTGAACAACCTGCTTTATTCCAGGGGAAAAAGTTTGATCTCCGTTATGTAGTACTGGTTCGGAGCATGCACCCTTTGGAGATATTCCTGTCAGATTGTTTCTGGGTATACTGATTCTTCTTTCTTTATTTTATTCTTATGCTCTTAAAAGGTTAAACTCTAATGGCAATGAATTTTATGTTTTATTTCAGGTTAGGATAGCTAACAACCAATATTCTTTGGCCAAAAGTAGTCTTTTTGAGTATGAAACTCATTTCACTGTTATGGTATGTCTTGAATTCCATATTGTTTAGCAACAAGATGTTTGGTTGTGCTTCCCTTTTTCTTATCAAGTTTTAACATATTCAATCAATGCATGTTCTCAGAATTATCGTGGAAGAATAAATCACAAGAATATTAAGGATTTTGTGAGGGAATTTGAGGAAGAACATCAAGGTAATGAGGGCTGCCACATTCTATAGATGAATTTTTAGAGGCTTCCAGAGCATATTATATATTTTGAATGAATTTTTTTATACAAATTCATGTCTCTGTATCTCTTGCAGTTAAATGGTTGGATATACATGCAAGAGTAAGGACTATGATTCGATCGGTGTTTGAGGCAGCTGCGGTTGCACATCCTGAGATGCATAGTCCAACATCAAGGGCAATTTACGGTGTAGATGTCATGTTAGACAGCTCTTTCCAACCTAAGTTACTAGAGGTTGGTAACCGAAACAGTGGTTCTCATTTGATGCTCCTTTCAATACCAAATTTTTTTATATTGACCTGAACATCTTGATTCAAGAATACAAATTTGTAATGTACTATTTGTTTAATGCTGAATTATCTGATTAGGATGCATTTCACCATACGAATTATCGCATTACATGTATGACTTGTATTTTATATTGCAGGTGACTTACTGCCCTGATTGTACACGAGCTTGCAAATATGACATGGATACCGTTGTTGGAGAAGGAGGTATTGCCAAAGGTTGTGATTTCTTCAACAATGTTTTCAAGTGTCTCTTTTTGAATGAAATTTCTCAGGTTAGTCAATTGTAATCTGTTCATCACAGAGATTTGACATCCTTAATTAAAGCCTTCTTCGACAACGGACTACAGGTTCTTTTAGCAAGGCCTGGAAATTAG
Protein sequence

>MS.gene025221.t1

MVAMATTTKRIETFEDFAKVHGILLAASGLPESLHRRLFHKLSTETFDGGEHFQIEPCEENRMRRLVLTSDSMAKDSNVFLVDHAWTFRLSDAYKQLREVPGLAERMGSLMCVDIDLDSETEVNGVDDDDVSGEKVSVVEMLESEVREVKEKGDGTLRWLELDGLDIDDDMFLSLDLPARFPNLVALSLYGNKLTRPELIIQEVIKFKHLKGLWLNNNPVLQNCDGDLAGIILKELPELEIYNSTFTSNFGEWALGFCAEIYGKDNPSDQADSPLLSVSNLDLSNRNIHNLINKDFSPICLPSLSYLNIRGNPLEQSSVTDLLELLRRFSCLRSLEVDIPGPLGGSAIEILESLPNISELNGICASKILESGKHVIDSVLLPRLPEWTPDEPIHDRIISAMWQYLMTYRLADEEKLDETSVWYVMDEFGSALRHSDEPNFRVAPFLFMPEGNLASAVSFSILWPTRNVWKGDECTRDFLLGIGEDKQRSARLTAWFNTPENYFIQEYEKYNQRLQSTSLIPPTVQSSESRSIRPHDGRPLRVYTDIPHVEEYLTHPEFAIIKDPKDADIIWTCVQVDEELKKATGITDQQYINQFPFEACLVMKHHLAETIQKAHGSPQWLQPTYNLETHLSQLIGDYYVRKREGLDNLWILKPWNMARTIDTTVTDNLPAIIRLMETGPKICQKYIEQPALFQGKKFDLRYVVLVRSMHPLEIFLSDCFWVRIANNQYSLAKSSLFEYETHFTVMNYRGRINHKNIKDFVREFEEEHQVKWLDIHARVRTMIRSVFEAAAVAHPEMHSPTSRAIYGVDVMLDSSFQPKLLEVTYCPDCTRACKYDMDTVVGEGGIAKGCDFFNNVFKCLFLNEISQVLLARPGN