AlfalfaGEDB | MS.gene02544

M. sativa cultivar XinJiangDaYe / MS.gene02544

KEGG: K10523
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00651.34

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02544.t1	XP_013461323.1	99.5	209	1	0	142	350	45	253	6.80E-109	404.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02544.t1	Q6DBN1	68.1	216	62	2	138	346	19	234	1.6e-74	281.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02544.t1	I3T836	99.5	209	1	0	142	350	45	253	4.9e-109	404.1

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene02544.t1	TR	TRAF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene028078	MS.gene02544	PPI
MS.gene028080	MS.gene02544	PPI
MS.gene070084	MS.gene02544	PPI
MS.gene72831	MS.gene02544	PPI
MS.gene84986	MS.gene02544	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02544.t1	MTR_3g088660	98.592	213	3	0	138	350	41	253	2.33e-155	436
MS.gene02544.t1	MTR_1g022305	61.321	106	27	3	4	106	368	462	1.27e-26	110
MS.gene02544.t1	MTR_3g072160	27.778	198	134	4	140	333	29	221	2.14e-14	72.4
MS.gene02544.t1	MTR_3g072160	27.778	198	134	4	140	333	66	258	1.04e-13	70.9
MS.gene02544.t1	MTR_7g094900	26.490	151	107	1	174	324	542	688	2.20e-13	71.6
MS.gene02544.t1	MTR_7g094910	26.490	151	107	1	174	324	523	669	2.36e-13	71.6
MS.gene02544.t1	MTR_3g088560	30.769	143	94	2	167	308	29	167	2.62e-13	68.6
MS.gene02544.t1	MTR_3g088560	30.769	143	94	2	167	308	81	219	3.99e-13	68.9
MS.gene02544.t1	MTR_7g094490	29.012	162	98	4	194	340	220	379	8.45e-13	69.3
MS.gene02544.t1	MTR_3g088560	30.556	144	95	2	166	308	95	234	1.15e-12	67.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene02544.t1	AT4G08455	68.056	216	62	2	138	346	46	261	1.09e-101	301
MS.gene02544.t1	AT1G01640	31.902	163	108	2	177	338	24	184	7.58e-19	84.3
MS.gene02544.t1	AT1G01640	31.902	163	108	2	177	338	3	163	7.68e-19	83.6
MS.gene02544.t1	AT3G56230	29.592	196	127	5	146	338	86	273	1.43e-18	85.1
MS.gene02544.t1	AT4G37610	32.716	162	97	4	192	343	67	226	5.05e-13	69.7
MS.gene02544.t1	AT3G06190	25.333	225	136	6	130	340	168	374	1.06e-12	68.9
MS.gene02544.t1	AT1G21780	32.031	128	78	3	155	279	139	260	3.54e-11	63.9
MS.gene02544.t1	AT1G21780	32.031	128	78	3	155	279	139	260	3.54e-11	63.9
MS.gene02544.t1	AT3G06190	25.728	206	123	5	130	323	168	355	5.49e-11	63.5
MS.gene02544.t1	AT2G40450	29.775	178	117	3	180	349	26	203	6.33e-11	61.6

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Find 78 sgRNAs with CRISPR-Local

Find 416 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTGTAATGACAGTCTTT+AGG	0.349021	3.2:+75625670	None:intergenic
AAATGAAGATTATACGGAGT+TGG	0.527148	3.2:-75620563	MS.gene02544:CDS
AACGGCCTTGTGAGCGGGAA+TGG	0.421444	3.2:+75621338	None:intergenic
AACTAATCCTCGTTTAGTTG+TGG	0.493649	3.2:-75620536	MS.gene02544:CDS
AACTATCACCGGACGGAAGA+AGG	0.480804	3.2:+75621370	None:intergenic
AAGAAGGACAAGATCGGTGG+TGG	0.529952	3.2:+75621386	None:intergenic
ACGAGAACGGCCTTGTGAGC+GGG	0.514027	3.2:+75621333	None:intergenic
ACTGAGAGAACTAGTCTTAG+CGG	0.505152	3.2:-75625592	MS.gene02544:CDS
AGAATGATATGGAGGAAAGC+CGG	0.570838	3.2:-75620876	MS.gene02544:CDS
AGACTTTGGATTTGAGGTCT+TGG	0.386775	3.2:+75621445	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACATCGCTAAAAACGTAA+AGG	-	chr3.2:75621967-75621986	MS.gene02544:intron	30.0%
AAAGTGTAATGACAGTCTTT+AGG	+	chr3.2:75621260-75621279	None:intergenic	30.0%
AAATAAAAAGGTGAAGAGAG+AGG	+	chr3.2:75625626-75625645	None:intergenic	30.0%
AAATGAAGATTATACGGAGT+TGG	-	chr3.2:75626364-75626383	MS.gene02544:CDS	30.0%
AACATCGCTAAAAACGTAAA+GGG	-	chr3.2:75621968-75621987	MS.gene02544:intron	30.0%
AAGAAAGTTTAGAGAGAAGT+TGG	-	chr3.2:75622423-75622442	MS.gene02544:intron	30.0%
AAGAGAAAAAAGATGTAGAG+GGG	-	chr3.2:75622345-75622364	MS.gene02544:intron	30.0%
AATAAAAAGGTGAAGAGAGA+GGG	+	chr3.2:75625625-75625644	None:intergenic	30.0%
ACAGAAACTTGATAAACAAC+AGG	+	chr3.2:75623979-75623998	None:intergenic	30.0%
ACCAATAACCCTATATATCA+AGG	+	chr3.2:75622519-75622538	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	75620477	75626472	75620477	ID=MS.gene02544
chr3.2	mRNA	75620477	75626472	75620477	ID=MS.gene02544.t1;Parent=MS.gene02544
chr3.2	exon	75626470	75626472	75626470	ID=MS.gene02544.t1.exon1;Parent=MS.gene02544.t1
chr3.2	CDS	75626470	75626472	75626470	ID=cds.MS.gene02544.t1;Parent=MS.gene02544.t1
chr3.2	exon	75626329	75626397	75626329	ID=MS.gene02544.t1.exon2;Parent=MS.gene02544.t1
chr3.2	CDS	75626329	75626397	75626329	ID=cds.MS.gene02544.t1;Parent=MS.gene02544.t1
chr3.2	exon	75625520	75625862	75625520	ID=MS.gene02544.t1.exon3;Parent=MS.gene02544.t1
chr3.2	CDS	75625520	75625862	75625520	ID=cds.MS.gene02544.t1;Parent=MS.gene02544.t1
chr3.2	exon	75621335	75621516	75621335	ID=MS.gene02544.t1.exon4;Parent=MS.gene02544.t1
chr3.2	CDS	75621335	75621516	75621335	ID=cds.MS.gene02544.t1;Parent=MS.gene02544.t1
chr3.2	exon	75620477	75620932	75620477	ID=MS.gene02544.t1.exon5;Parent=MS.gene02544.t1
chr3.2	CDS	75620477	75620932	75620477	ID=cds.MS.gene02544.t1;Parent=MS.gene02544.t1

Gene Sequence

>MS.gene02544

ATGGTAAAGAGCTGTATTTTACCCGTATGCACACCATGCACACTGATGTTATTCATAATTTTTTATTCTATATAGGTTGATGTATCTCCCGAACTTCTGGACACACTGAAGCAGCAGCGTCGCTTTATGCCAGTAGAACTTAAGGTTTATCTTCTTCTCTCTTGTCCATTTAAATGCTATTTTGATATGTTAAGCCACTTATATTCAATGTTTTTAACATATGTTTTACTGCCTTGCTTCAAACGAAGTCATTCTATTATTATTCTTTCCCTATAGTGAGTTAAACTTAGTTAATGTGTCTTGGTGAGGTTGTCAAAGATGTTATACTCTTTATGAGTGGATGGTCACTATGGTGGCTTTTGATACACTATTTTTGCGTTTTTTAGATTTTCAATAATTTAGTTCTAGTTAGCTCCGACTCGGAATAAACTCACAGGAAGTGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTATATATATATATATATATATATATATATGCAGATTTTAGGATGTTTATCTTTTATTTTCTCATTAAATTTTGTACTGTTTAGATTTTTGATAATTTTGTTCTAATTTGTGCTAAAAAAAAACTCACAGGAAACTGAGTTTGCGACTGAAAATTCAGCTGATGGCGATATTGTTGTTATCAAAACAAATGATGGTGTTGCTCACAATGTTGAACGAGTTTTTGCGGGGAAGATCTGGACAAAGTATGGCCTTGCAACATCCGAAACTGAATTTGAACTTCCCAATGTGGGAAGCGATGTCCTAAAGACTGTCATTACACTTTTAAAACAAGCCCAAGCATTTCAGAAGATCGACGTGGGAGAGTTTACAAATGATCAACTGAGAGAACTAGTCTTAGCGGTTTATCACCTCGGCTTCAGTGAGCTTCAAGCTTCCTTATGTCAGGTCATTGCTGATAGATTAGAAAATCAAAGTACAGCTTATATGCGGACATATTTTGGAATTCAAAAGGAACACACACAAGAGGAAGAGAGCAAGTTAAAGGAGGATGCTTGCACATTGGAAAATGATGATTGATGAGTTATGATTTGATTTGAAGATTGTTTACAATCATATTGTGCTAAATTTGCTTATGTATGTTTGGGTTCACTTTTTGTGCTAACGTTCTAATATTCATAGGGTAAAGGGATAAATAATACATGGATGACCTCTTTGGAGATATTCTTTGATTCTGCATCAGCGATGTGTGCGGCGTTTCTTTGTTTTTTGTTTGGGAAAATGCATTGACCCACGGCGAAACAATGAGAAATTTAGTTAATATAGTATAGTATAAATGTGCAATGTTGTAATTAAATCTTACCGCAACGACCGCAATCCTTAGAACTGAAACCGTCTACTAAGAAAACCTAGAAATCGCAAAATCCTTTATTCGACTTGACCTTGACACTAATTTTTTTGACAAAAACAAAACGATATTCATTCATTCAAATCGATAGAGTACATCAGATACAAACATCGCTAAAAACGTAAAGGGTGAATCTGCGAACAAAGTCATAGCACCCAAGTTAATAGCTGCGAACAAACTCATAGCACCCAAGTTAATAGCATAAAACGGCAAAATGCCTACAAAAAATATGATAAAACCTAAGTCATCGAAATTCACATGCTTCCGGATCTGCAACGTTGACGCCCAAATCATTTGTTGAATTTGTAATTGACTGATGCCGATCGTTCAATAGAAACTAAACGAACACCACGACAAGACGCGAAATCAAACGCCGCACAAGACGACGAACAACACAACGCCACGCTTAGACGACGACATAACAAAAAAACTTAAAAGAAGAAACTTGATCTATGTGAAAACCACTTATTTAGATTGAAAGAAAAGAGAAAAAAGATGTAGAGGGGTGATTCTAGGTCAAAAAATGACCTAAAACCACCCCTCCTCGATGAACGAAGGGGAAAGAAAGTTTAGAGAGAAGTTGGAGTTTCTCTCACTAGAAAACTAGGGCCGGCTGTTTTTACCACGTGACACCGACCTTGACACTAATTAAAACCTTGATATATAGGGTTATTGGTATATCCCATTGATGCAAAATTTCATATCTTGACCCGTATTGAAACGAGATTATGAGACTATCTTTAATAGCTACTGCGGACCAACTAATATATCTTTGCAATTATGATCTTTAAGTGTGTTTCTGAAACAAAATGTTTGTAAAAGTAGTTTTTTTTTTTTGAGCAATTTATGTTGCTCAAAGTCATGACTCTTATTTAAGAGCAACGACTTGGGGGCTACTGTTTTGGGCCGAGCTAAAAAACATAAAAGGCCCAATTTCATAGGTCCAGTTCATACATACCATATATCGCACTTGGGTCAACTTCTTCCTAACACACCATCTCCACGAGGGAGGTGTTCTTAACACTTTGGGAGACCAAATTCCAACTGATTTGTAACGTGTCTAGATGGTTACAATTCCCTCTCCACGAGGGAGGTGTTATTAACACTTTGAGATACCAGCTCATGCGCCTCTATAATGAGACTTTTCACTGTCCTCATAGTTACGAAATCTTTCAACCCTAAACCCACATTCTTGCTCTCTAAACTGACTTGAGCGTCAGAGTGTCTCCTCGCTGCTCCAGAGCTGACAAACACTTAGAACCACCGCACTGGACGTCATCATTCTAATTTGGTAAGATCAATTACGAACTAATTTTTAAAATTAATATTACCAATATGTTTGTTTTTTATATAATTAATCTAGTTGATAGAAATTCATATTCAAACTCTCGCGATGAATATAGTATACATTGTTTTTAGAGAATATATTATGAATTATCGTTGCCGCTAAAAGAAAAAAGTAATTATATTTGTACAACTATTTAATTACAATTCTTGTGACAATTGATCAACAATAATAGAGAGAGAGAGATGAAAAAAAAAATAAGAACATGATGTAAGTATGTAAGAGTGTTTATTCGAAACTTGTCACAAAATAGTTAAACATTATTGGGTTAGACAATATTTTGTCCCCTGCCATTTAGGTGAGTTTTGAAAAACGAGTGAACCATCAAATTCGTCCCTGAATTTTCACGTGTCGGCCAACTTCGTCCCTCAATTATGTGAAATATCAACTTAGTCCCTGAATTTGTCAGACGTCCATCAAAAAGATCCCTCCGTCCAGGTGCTCCGTTAGCCATGCTGATCTGTCGCCCTACATGTTGTGTTGTCTTGTACAAGTGGCAAGAAGGAAGCCACGTGTTCAGGGACTAATTTGATTGAATTGGAAAAATTTGAGTTTTCACTTTTAGTTTATTTTTTTCTGCTTTTCCCATTTTGCCCCTGCATTACCCATACCTCCTTCTTCACATTTCAGAACATCAAAAACGTCCTTGACCCTTCTTCTTCCCGCTAAAACTTCCTCCATTTTCACCCCTCCAAAATCATCATCATCTCCGGCGATTCTGACGTTTTTGAAGTTTGTGTGTTCGGCTGTGATTAAAGGTAACGTCTTTCCTGTTGTTTATCAAGTTTCTGTGTTCGTATTCACCTTGTTTTTGTCCCTTTTTTTGTGTTTTCGTTTTAAGTTTTGATCATTTTTATTTTGTAATTTCTCAGACTTATGACTCATGCCTATGACTTATGCCTATGTAATATTTCAGTTTCAATTTGTGTGTTCTGCCTTTTTTACTTTATTTTGTACACGTATGTTTCAAAGATAAATTTGATATCTGATTAAACAATTCAGGGACTTATTTGATGGTTTTTTAATATAACGTAGGGATTTATTTGATAGATAGAGAAATAATTCAGGGACTAATTTGATGGCAATATTATTATAGAAAGATTACATTTCTCAATCATCTTCAACCTTTTTTCATTAGTTAAACCAACCTTTGGCAGAGGACAAGCATAGAATGGTCTTCCTAGATTTTTTGAAGTATTGGATTTTCTAACAACACAAATTGTAGGACACCAATATTCAAGCACTCTCACAACACCATTAAACTTGGAGCTTGAAGATGAAGCTTGACTTTCATAACCGTTTTTTCCATATTCCCACCCCTCATCATTTCAGATTCAATTTCAATAATTTTGCTTCAAGAAATTTTGAGGATTTTGGCTTTCAGATTCGATACGAAAAAAATTAGAGTTTGAAGTTTTTTAAATTGGGGCAGAACTGGACCACTTATTATTAGAGGAAATTTTCGTGATTATAGAGTGTAGATTGCAGATTGTGTAATTAGGGTTCCAATTTTATACAATTGGGGAAGAAAGAGCCGTTGGGGAAGAAAAGAGCCATTTAAGAAGAAAGCCAAATCATTTTATGCAAATCAATCAATTCAGTCCTTCAAAATGTTTGTTATTATCGGCCAAATTAGTCCCTGCCTACGTGGCGTCTGACATGTGAGAGGTTAACAGCCACGTCAGCCCCGTTAACGCTCCGTTTGAACAGAAGGACTAACTTGATTGACATTTAACAAATTCAGGGATTAAATTGATATTTCACATAATTGAGGGACGAAGTTGGCCGACCCATAAACATTCAGGGACGAAGTTGATGATTTACTCTTTGAAAAACCCTGTAAAAATATGTTAGATCTTCCCTTATCATTCAAAGATTTCATCATAAACGACCTTATCATTCAAATTTCAAAACTCGTCTATCTAAATAGTAAGGGAGGAAAATTATTTAACCAAATATTATTTGTCAAAAGAAAAGAAAAATAGATAAAATGGAAAAAGTGTATGTATGTGAAATGAGAGAGACCGTGTGACGCCGAAAGAGAAAACCCTTTTCCATCCAATATCCATCATCAATCGCATCGATCGAATCGATCAGATAGAATGCCACGGCACCGTTGCACAGTGTACTCCGAGAGCGATTACGACGACGACGACGATAACGTCACCATGAGATGTCTCTCTTGCGAGGACGATTACGACCCCGACGACGCCGGTACCTGCAAAGAATGCTACGACGAAGCCAACGAAACCGAAGAGGAATTCAAACGTCAGATCCAAGACCTCAAATCCAAAGTCTCCTTCCTCACTCTCGCTTCTCCTCTTGATGCTCCTTCCACCACCGATCTTGTCCTTCTTCCGTCCGGTGATAGTTTCTCTCCTTCCATTCCCGCTCACAAGGCCGTTCTCGTCAGTACCCTCTCTCTTCACCTTTTTATTTTTATTTTTTATTCTTTAATTTTTTATAGTGATGCTGCAAATTATGAAAATGTTAAATAATAATAACCATGTGGTTAGGTTGAAGTGGTTAACGAATTTCGGTTAAGAGTTAAGGACTAATTGTTCGGAAGAATCCCATGATAGGAACTTGAATATGTTAATTACATGAAAAATATGTTGATTACCCTTCTATTATGTATTGCTTATCTTGTCCACAATCAAAACGGGAGTATAATGGTGTATTATGGATATGTGTTTAAGACTTAATCCACTGATGATTTAATTTAAAGTGTACTATGGTGCCCTTACTCAAATGATTTTAAAGCTGTTAGCTGTTCACAATGAAACTCACATGACGCGATGTTTGAATAGGTTAGTCGCTCTCCAGTTTTCAGAGCCATGCTTGAGAATGATATGGAGGAAAGCCGGAGTGGCACCATCAAGATTGCTGATGTTTCGTACGATGCTCTTCGTGCTTTTGTTAACTACTTGTACACTGCTGAAGCATGCCTTGATAACCAAATGGCCTGTAACCTATTAGTTTTAGGAGAAAAGTATCAGGTGAAGCATCTTAAGGCGTATTGTGAGAAGTATTTGATCTCCAAACTGAATTGGGAGAAAGCTGTTGTCAACTTTGCCTTTGCACATCAACACAATGCTAACCAATTGCAAGATGCAGCTTTGGCAGTGATCATGGAGAATATGGACAACCTCACTAAAAATGAAGATTATACGGAGTTGGTGGAAACTAATCCTCGTTTAGTTGTGGAAATATATGAAGCTTATCTTGCCAAACAGGTTAATACTGCAGCCACGCTAGGGTTATAG

Protein sequence

>MS.gene02544.t1

MVDVSPELLDTLKQQRRFMPVELKETEFATENSADGDIVVIKTNDGVAHNVERVFAGKIWTKYGLATSETEFELPNVGSDVLKTVITLLKQAQAFQKIDVGEFTNDQLRELVLAVYHLGFSELQASLCQVIADRLENQKCYDEANETEEEFKRQIQDLKSKVSFLTLASPLDAPSTTDLVLLPSGDSFSPSIPAHKAVLVSRSPVFRAMLENDMEESRSGTIKIADVSYDALRAFVNYLYTAEACLDNQMACNLLVLGEKYQVKHLKAYCEKYLISKLNWEKAVVNFAFAHQHNANQLQDAALAVIMENMDNLTKNEDYTELVETNPRLVVEIYEAYLAKQVNTAATLGL