Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene02595.t1 | XP_013461323.1 | 85.7 | 231 | 2 | 1 | 1 | 200 | 1 | 231 | 3.40E-89 | 337.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene02595.t1 | Q6DBN1 | 53.7 | 216 | 62 | 3 | 23 | 200 | 1 | 216 | 5.6e-48 | 192.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene02595.t1 | I3T836 | 85.7 | 231 | 2 | 1 | 1 | 200 | 1 | 231 | 2.5e-89 | 337.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene02595.t1 | TR | TRAF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene02595 | MS.gene72831 | PPI |
| MS.gene02595 | MS.gene070084 | PPI |
| MS.gene028080 | MS.gene02595 | PPI |
| MS.gene028078 | MS.gene02595 | PPI |
| MS.gene84986 | MS.gene02595 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene02595.t1 | MTR_3g088660 | 85.714 | 231 | 2 | 1 | 1 | 200 | 1 | 231 | 3.02e-142 | 397 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene02595.t1 | AT4G08455 | 55.882 | 238 | 64 | 4 | 4 | 200 | 6 | 243 | 1.70e-82 | 246 |
Find 55 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGCAGGTACCGGCGTCGTC+GGG | 0.313630 | 3.3:+74550024 | None:intergenic |
| TACTTTCTCTCCTTCCATTC+CGG | 0.321591 | 3.3:-74549857 | MS.gene02595:CDS |
| TCAAGCATGGCTCTGAAAAC+TGG | 0.323355 | 3.3:+74549385 | None:intergenic |
| GGCAGTGATCATGGAGAATA+TGG | 0.334507 | 3.3:-74549084 | MS.gene02595:CDS |
| CTAATAGGTTACAGGCCATT+TGG | 0.355315 | 3.3:+74549251 | None:intergenic |
| CCAATTGCAAGATGCAGCTT+TGG | 0.375309 | 3.3:-74549105 | MS.gene02595:CDS |
| CTGACGTTTGAATTCCTCTT+CGG | 0.381082 | 3.3:+74549975 | None:intergenic |
| AGACTTTGGATTTGAGGTCT+TGG | 0.386775 | 3.3:+74549950 | None:intergenic |
| GGAAGGAGACTTTGGATTTG+AGG | 0.393809 | 3.3:+74549944 | None:intergenic |
| CCAAAGCTGCATCTTGCAAT+TGG | 0.405862 | 3.3:+74549105 | None:intergenic |
| TACAGGCCATTTGGTTATCA+AGG | 0.418138 | 3.3:+74549260 | None:intergenic |
| CGAAACATCAGCAATCTTGA+TGG | 0.418642 | 3.3:+74549333 | None:intergenic |
| GAGAGTGAGGAAGGAGACTT+TGG | 0.418723 | 3.3:+74549936 | None:intergenic |
| TGCAGCTTTGGCAGTGATCA+TGG | 0.425358 | 3.3:-74549093 | MS.gene02595:CDS |
| TTCGTCGTAGCATTCTTTGC+AGG | 0.425478 | 3.3:+74550008 | None:intergenic |
| AGCCATGCTTGAGAATGATA+TGG | 0.435569 | 3.3:-74549374 | MS.gene02595:CDS |
| TTTGCAGGTACCGGCGTCGT+CGG | 0.460574 | 3.3:+74550023 | None:intergenic |
| GAATTCCTCTTCGGTTTCGT+TGG | 0.472827 | 3.3:+74549984 | None:intergenic |
| TCCTTCCATTCCGGCTCACA+AGG | 0.473877 | 3.3:-74549848 | MS.gene02595:CDS |
| AACGGCCTTGTGAGCCGGAA+TGG | 0.487905 | 3.3:+74549843 | None:intergenic |
| AGCTGCATCTTGCAATTGGT+TGG | 0.493566 | 3.3:+74549109 | None:intergenic |
| GTAGCATTCTTTGCAGGTAC+CGG | 0.496432 | 3.3:+74550014 | None:intergenic |
| CTCCATATCATTCTCAAGCA+TGG | 0.507732 | 3.3:+74549372 | None:intergenic |
| ACGAGAACGGCCTTGTGAGC+CGG | 0.512199 | 3.3:+74549838 | None:intergenic |
| AGGAGAAGCGAGAGTGAGGA+AGG | 0.513816 | 3.3:+74549927 | None:intergenic |
| GTCGTCGTCGTAATCGCTCT+CGG | 0.517038 | 3.3:+74550095 | None:intergenic |
| TGAAGATTACACGGAGTTGG+TGG | 0.522112 | 3.3:-74549045 | MS.gene02595:CDS |
| GCCTTGTGAGCCGGAATGGA+AGG | 0.523187 | 3.3:+74549847 | None:intergenic |
| AAATGAAGATTACACGGAGT+TGG | 0.527013 | 3.3:-74549048 | MS.gene02595:CDS |
| AAGGAGAACAAGATCGGTGG+TGG | 0.529952 | 3.3:+74549891 | None:intergenic |
| AGCATGCCTTGATAACCAAA+TGG | 0.551457 | 3.3:-74549266 | MS.gene02595:CDS |
| GATCTTGTTCTCCTTCCGTC+CGG | 0.554395 | 3.3:-74549883 | MS.gene02595:CDS |
| CGAAGCCAACGAAACCGAAG+AGG | 0.562358 | 3.3:-74549989 | MS.gene02595:CDS |
| TACACTGTGCAACGGTGCCG+TGG | 0.563730 | 3.3:+74550120 | None:intergenic |
| AGAAAGTATCACCGGACGGA+AGG | 0.567541 | 3.3:+74549872 | None:intergenic |
| AGAATGATATGGAGGAAAGC+CGG | 0.570838 | 3.3:-74549363 | MS.gene02595:CDS |
| CAAGAGGAGAAGCGAGAGTG+AGG | 0.574599 | 3.3:+74549923 | None:intergenic |
| GGCATTGTGTTGATGTGCAA+AGG | 0.583175 | 3.3:+74549130 | None:intergenic |
| TGCAGGTACCGGCGTCGTCG+GGG | 0.583425 | 3.3:+74550025 | None:intergenic |
| CAATCTTGATGGTGCCACTC+CGG | 0.589211 | 3.3:+74549344 | None:intergenic |
| CTCGCAAGAGAGACATCTCA+TGG | 0.590091 | 3.3:+74550059 | None:intergenic |
| AGAACAAGATCGGTGGTGGA+AGG | 0.595036 | 3.3:+74549895 | None:intergenic |
| GGAAGGAGAGAAAGTATCAC+CGG | 0.597919 | 3.3:+74549864 | None:intergenic |
| GATTACGACCCCGACGACGC+CGG | 0.624267 | 3.3:-74550033 | MS.gene02595:CDS |
| GGACGGAAGGAGAACAAGAT+CGG | 0.626611 | 3.3:+74549885 | None:intergenic |
| ATATATTTCCACAACTAACG+AGG | 0.630781 | 3.3:+74549014 | None:intergenic |
| CATGAGATGTCTCTCTTGCG+AGG | 0.633418 | 3.3:-74550058 | MS.gene02595:CDS |
| AGGGAGGGTACTGACGAGAA+CGG | 0.641189 | 3.3:+74549825 | None:intergenic |
| CATGCTTGAGAATGATATGG+AGG | 0.646700 | 3.3:-74549371 | MS.gene02595:CDS |
| GGAGAGAAAGTATCACCGGA+CGG | 0.661533 | 3.3:+74549868 | None:intergenic |
| GTGGTGGAAGGAGCATCAAG+AGG | 0.662081 | 3.3:+74549907 | None:intergenic |
| TCTCGGAGTACACTGTGCAA+CGG | 0.678301 | 3.3:+74550112 | None:intergenic |
| GATATGGAGGAAAGCCGGAG+TGG | 0.686483 | 3.3:-74549358 | MS.gene02595:CDS |
| CGGAAGGAGAACAAGATCGG+TGG | 0.688815 | 3.3:+74549888 | None:intergenic |
| ATCGATCAGATAGAATGCCA+CGG | 0.689432 | 3.3:-74550137 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTTTAAATTAAATCATCAG+TGG | + | chr3.3:74549673-74549692 | None:intergenic | 20.0% |
| !! | GTTAAATAATAATAACCATG+TGG | - | chr3.3:74549422-74549441 | MS.gene02595:intron | 20.0% |
| !!! | GAATATGTTTTTTTGAAGAA+GGG | - | chr3.3:74549521-74549540 | MS.gene02595:intron | 20.0% |
| !!! | TGAATATGTTTTTTTGAAGA+AGG | - | chr3.3:74549520-74549539 | MS.gene02595:intron | 20.0% |
| ! | AAGCAATACATAATAGAAGA+AGG | + | chr3.3:74549587-74549606 | None:intergenic | 25.0% |
| ! | AGCAATACATAATAGAAGAA+GGG | + | chr3.3:74549586-74549605 | None:intergenic | 25.0% |
| ! | ATAATAATAACCATGTGGTT+AGG | - | chr3.3:74549427-74549446 | MS.gene02595:intron | 25.0% |
| ! | CACTAAAAATGAAGATTACA+CGG | - | chr3.3:74550097-74550116 | MS.gene02595:CDS | 25.0% |
| !! | CTTTTTCTCCTAAAACTAAT+AGG | + | chr3.3:74549918-74549937 | None:intergenic | 25.0% |
| !!! | TGTAATCTTCATTTTTAGTG+AGG | + | chr3.3:74550097-74550116 | None:intergenic | 25.0% |
| !!! | TTTTAGGAGAAAAAGTAATC+AGG | - | chr3.3:74549923-74549942 | MS.gene02595:CDS | 25.0% |
| AATAAAAAAGGTGAAGAGAG+AGG | + | chr3.3:74549349-74549368 | None:intergenic | 30.0% | |
| AGAGTTAAGGACTAATTGTT+CGG | - | chr3.3:74549476-74549495 | MS.gene02595:intron | 30.0% | |
| ATAAAAAAGGTGAAGAGAGA+GGG | + | chr3.3:74549348-74549367 | None:intergenic | 30.0% | |
| ATTTGATCTCCAAACTGAAT+TGG | - | chr3.3:74549976-74549995 | MS.gene02595:CDS | 30.0% | |
| TATCTTGTCCACAATCAAAA+CGG | - | chr3.3:74549606-74549625 | MS.gene02595:intron | 30.0% | |
| TCCTAAAACTAATAGGTTAC+AGG | + | chr3.3:74549911-74549930 | None:intergenic | 30.0% | |
| TTGAAGTGGTTAACGAATTT+CGG | - | chr3.3:74549450-74549469 | MS.gene02595:intron | 30.0% | |
| TTTGATCTCCAAACTGAATT+GGG | - | chr3.3:74549977-74549996 | MS.gene02595:CDS | 30.0% | |
| ! | ACATATTCAAGTTCCTATCA+TGG | + | chr3.3:74549509-74549528 | None:intergenic | 30.0% |
| ! | CATATTCAAGTTCCTATCAT+GGG | + | chr3.3:74549508-74549527 | None:intergenic | 30.0% |
| !! | AGCTTTAAAATCATCGAGTA+AGG | + | chr3.3:74549707-74549726 | None:intergenic | 30.0% |
| !! | GCTTTAAAATCATCGAGTAA+GGG | + | chr3.3:74549706-74549725 | None:intergenic | 30.0% |
| AAATGAAGATTACACGGAGT+TGG | - | chr3.3:74550103-74550122 | MS.gene02595:CDS | 35.0% | |
| ATCTTGTCCACAATCAAAAC+GGG | - | chr3.3:74549607-74549626 | MS.gene02595:intron | 35.0% | |
| CGAATTTCGGTTAAGAGTTA+AGG | - | chr3.3:74549463-74549482 | MS.gene02595:intron | 35.0% | |
| ! | CAATCAAAACGGGAGTATAA+TGG | - | chr3.3:74549617-74549636 | MS.gene02595:intron | 35.0% |
| ! | GGGAGTATAATGGTGTATTA+TGG | - | chr3.3:74549627-74549646 | MS.gene02595:intron | 35.0% |
| ! | TAATCAGGTGAAGCATCTTA+AGG | - | chr3.3:74549938-74549957 | MS.gene02595:CDS | 35.0% |
| !!! | GCCTGTAACCTATTAGTTTT+AGG | - | chr3.3:74549907-74549926 | MS.gene02595:CDS | 35.0% |
| !!! | TTATACTCCCGTTTTGATTG+TGG | + | chr3.3:74549617-74549636 | None:intergenic | 35.0% |
| AGAATGATATGGAGGAAAGC+CGG | - | chr3.3:74549788-74549807 | MS.gene02595:intron | 40.0% | |
| AGCCATGCTTGAGAATGATA+TGG | - | chr3.3:74549777-74549796 | MS.gene02595:intron | 40.0% | |
| AGCTTTCTCCCAATTCAGTT+TGG | + | chr3.3:74549988-74550007 | None:intergenic | 40.0% | |
| CATGCTTGAGAATGATATGG+AGG | - | chr3.3:74549780-74549799 | MS.gene02595:intron | 40.0% | |
| CGAAACATCAGCAATCTTGA+TGG | + | chr3.3:74549821-74549840 | None:intergenic | 40.0% | |
| CTAATAGGTTACAGGCCATT+TGG | + | chr3.3:74549903-74549922 | None:intergenic | 40.0% | |
| CTCCATATCATTCTCAAGCA+TGG | + | chr3.3:74549782-74549801 | None:intergenic | 40.0% | |
| CTGACGTTTGAATTCCTCTT+CGG | + | chr3.3:74549179-74549198 | None:intergenic | 40.0% | |
| CTGTTACGCGATGTTTGAAT+AGG | - | chr3.3:74549732-74549751 | MS.gene02595:intron | 40.0% | |
| TACAGGCCATTTGGTTATCA+AGG | + | chr3.3:74549894-74549913 | None:intergenic | 40.0% | |
| TACTTTCTCTCCTTCCATTC+CGG | - | chr3.3:74549294-74549313 | MS.gene02595:CDS | 40.0% | |
| ! | AGACTTTGGATTTGAGGTCT+TGG | + | chr3.3:74549204-74549223 | None:intergenic | 40.0% |
| ! | AGCATGCCTTGATAACCAAA+TGG | - | chr3.3:74549885-74549904 | MS.gene02595:CDS | 40.0% |
| AAAAAGGTGAAGAGAGAGGG+AGG | + | chr3.3:74549345-74549364 | None:intergenic | 45.0% | |
| AAAAGGTGAAGAGAGAGGGA+GGG | + | chr3.3:74549344-74549363 | None:intergenic | 45.0% | |
| ACCACTTCAACCTAACCACA+TGG | + | chr3.3:74549440-74549459 | None:intergenic | 45.0% | |
| AGCTGCATCTTGCAATTGGT+TGG | + | chr3.3:74550045-74550064 | None:intergenic | 45.0% | |
| CCAAAGCTGCATCTTGCAAT+TGG | + | chr3.3:74550049-74550068 | None:intergenic | 45.0% | |
| CCAATTGCAAGATGCAGCTT+TGG | - | chr3.3:74550046-74550065 | MS.gene02595:CDS | 45.0% | |
| GAATTCCTCTTCGGTTTCGT+TGG | + | chr3.3:74549170-74549189 | None:intergenic | 45.0% | |
| GGAAGGAGACTTTGGATTTG+AGG | + | chr3.3:74549210-74549229 | None:intergenic | 45.0% | |
| GGCAGTGATCATGGAGAATA+TGG | - | chr3.3:74550067-74550086 | MS.gene02595:CDS | 45.0% | |
| GTAGCATTCTTTGCAGGTAC+CGG | + | chr3.3:74549140-74549159 | None:intergenic | 45.0% | |
| GTTCGGAAGAATCCCATGAT+AGG | - | chr3.3:74549493-74549512 | MS.gene02595:intron | 45.0% | |
| TGAAGATTACACGGAGTTGG+TGG | - | chr3.3:74550106-74550125 | MS.gene02595:CDS | 45.0% | |
| TTCGTCGTAGCATTCTTTGC+AGG | + | chr3.3:74549146-74549165 | None:intergenic | 45.0% | |
| ! | GGCATTGTGTTGATGTGCAA+AGG | + | chr3.3:74550024-74550043 | None:intergenic | 45.0% |
| !! | ACCATGTGGTTAGGTTGAAG+TGG | - | chr3.3:74549436-74549455 | MS.gene02595:intron | 45.0% |
| !! | GGAAGGAGAGAAAGTATCAC+CGG | + | chr3.3:74549290-74549309 | None:intergenic | 45.0% |
| !! | TCAAGCATGGCTCTGAAAAC+TGG | + | chr3.3:74549769-74549788 | None:intergenic | 45.0% |
| !! | AAGAAAAATAAAAATAAAAA+AGG | + | chr3.3:74549361-74549380 | None:intergenic | 5.0% |
| AAGGAGAACAAGATCGGTGG+TGG | + | chr3.3:74549263-74549282 | None:intergenic | 50.0% | |
| CATGAGATGTCTCTCTTGCG+AGG | - | chr3.3:74549093-74549112 | MS.gene02595:CDS | 50.0% | |
| CTCGCAAGAGAGACATCTCA+TGG | + | chr3.3:74549095-74549114 | None:intergenic | 50.0% | |
| GAGAGTGAGGAAGGAGACTT+TGG | + | chr3.3:74549218-74549237 | None:intergenic | 50.0% | |
| GATCTTGTTCTCCTTCCGTC+CGG | - | chr3.3:74549268-74549287 | MS.gene02595:CDS | 50.0% | |
| GGACGGAAGGAGAACAAGAT+CGG | + | chr3.3:74549269-74549288 | None:intergenic | 50.0% | |
| TCTCGGAGTACACTGTGCAA+CGG | + | chr3.3:74549042-74549061 | None:intergenic | 50.0% | |
| ! | TGCAGCTTTGGCAGTGATCA+TGG | - | chr3.3:74550058-74550077 | MS.gene02595:CDS | 50.0% |
| !! | AGAAAGTATCACCGGACGGA+AGG | + | chr3.3:74549282-74549301 | None:intergenic | 50.0% |
| !! | AGAACAAGATCGGTGGTGGA+AGG | + | chr3.3:74549259-74549278 | None:intergenic | 50.0% |
| !! | CAATCTTGATGGTGCCACTC+CGG | + | chr3.3:74549810-74549829 | None:intergenic | 50.0% |
| !! | GGAGAGAAAGTATCACCGGA+CGG | + | chr3.3:74549286-74549305 | None:intergenic | 50.0% |
| AGGAGAAGCGAGAGTGAGGA+AGG | + | chr3.3:74549227-74549246 | None:intergenic | 55.0% | |
| CAAGAGGAGAAGCGAGAGTG+AGG | + | chr3.3:74549231-74549250 | None:intergenic | 55.0% | |
| CGAAGCCAACGAAACCGAAG+AGG | - | chr3.3:74549162-74549181 | MS.gene02595:intron | 55.0% | |
| CGGAAGGAGAACAAGATCGG+TGG | + | chr3.3:74549266-74549285 | None:intergenic | 55.0% | |
| GATATGGAGGAAAGCCGGAG+TGG | - | chr3.3:74549793-74549812 | MS.gene02595:intron | 55.0% | |
| GTCGTCGTCGTAATCGCTCT+CGG | + | chr3.3:74549059-74549078 | None:intergenic | 55.0% | |
| TCCTTCCATTCCGGCTCACA+AGG | - | chr3.3:74549303-74549322 | MS.gene02595:CDS | 55.0% | |
| !! | AGGGAGGGTACTGACGAGAA+CGG | + | chr3.3:74549329-74549348 | None:intergenic | 55.0% |
| !! | GTGGTGGAAGGAGCATCAAG+AGG | + | chr3.3:74549247-74549266 | None:intergenic | 55.0% |
| GCCTTGTGAGCCGGAATGGA+AGG | + | chr3.3:74549307-74549326 | None:intergenic | 60.0% | |
| ! | AACGGCCTTGTGAGCCGGAA+TGG | + | chr3.3:74549311-74549330 | None:intergenic | 60.0% |
| ! | ACGAGAACGGCCTTGTGAGC+CGG | + | chr3.3:74549316-74549335 | None:intergenic | 60.0% |
| ! | TACACTGTGCAACGGTGCCG+TGG | + | chr3.3:74549034-74549053 | None:intergenic | 60.0% |
| !! | TTTGCAGGTACCGGCGTCGT+CGG | + | chr3.3:74549131-74549150 | None:intergenic | 60.0% |
| GATTACGACCCCGACGACGC+CGG | - | chr3.3:74549118-74549137 | MS.gene02595:CDS | 65.0% | |
| !! | TTGCAGGTACCGGCGTCGTC+GGG | + | chr3.3:74549130-74549149 | None:intergenic | 65.0% |
| !! | TGCAGGTACCGGCGTCGTCG+GGG | + | chr3.3:74549129-74549148 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 74549028 | 74550145 | 74549028 | ID=MS.gene02595 |
| chr3.3 | mRNA | 74549028 | 74550145 | 74549028 | ID=MS.gene02595.t1;Parent=MS.gene02595 |
| chr3.3 | exon | 74549840 | 74550145 | 74549840 | ID=MS.gene02595.t1.exon1;Parent=MS.gene02595.t1 |
| chr3.3 | CDS | 74549840 | 74550145 | 74549840 | ID=cds.MS.gene02595.t1;Parent=MS.gene02595.t1 |
| chr3.3 | exon | 74549263 | 74549419 | 74549263 | ID=MS.gene02595.t1.exon2;Parent=MS.gene02595.t1 |
| chr3.3 | CDS | 74549263 | 74549419 | 74549263 | ID=cds.MS.gene02595.t1;Parent=MS.gene02595.t1 |
| chr3.3 | exon | 74549028 | 74549167 | 74549028 | ID=MS.gene02595.t1.exon3;Parent=MS.gene02595.t1 |
| chr3.3 | CDS | 74549028 | 74549167 | 74549028 | ID=cds.MS.gene02595.t1;Parent=MS.gene02595.t1 |
| Gene Sequence |
| Protein sequence |
