AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene026777

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026777.t1 MTR_4g009040 92.069 290 18 1 23 312 45 329 0.0 543
MS.gene026777.t1 MTR_4g009040 91.724 290 15 1 23 312 45 325 0.0 539
MS.gene026777.t1 MTR_4g009690 91.409 291 16 1 23 313 45 326 0.0 516
MS.gene026777.t1 MTR_4g009690 91.065 291 16 2 23 313 45 325 0.0 509
MS.gene026777.t1 MTR_4g006940 41.379 290 152 2 25 310 51 326 1.34e-64 207
MS.gene026777.t1 MTR_2g104960 42.414 290 149 3 25 310 54 329 1.07e-62 202
MS.gene026777.t1 MTR_5g072620 38.889 288 160 3 28 310 43 319 3.37e-62 200
MS.gene026777.t1 MTR_4g006940 43.621 243 123 1 25 267 51 279 1.63e-57 187
MS.gene026777.t1 MTR_4g006940 43.621 243 123 1 25 267 51 279 1.63e-57 187
MS.gene026777.t1 MTR_2g028620 38.276 290 161 5 29 311 49 327 5.62e-55 182
MS.gene026777.t1 MTR_2g101330 35.517 290 175 4 26 312 41 321 4.86e-54 179
MS.gene026777.t1 MTR_3g005210 37.979 287 160 6 26 306 42 316 5.02e-53 176
MS.gene026777.t1 MTR_3g031650 36.014 286 171 2 28 310 43 319 5.43e-52 174
MS.gene026777.t1 MTR_3g005170 36.934 287 163 6 26 306 46 320 6.18e-52 174
MS.gene026777.t1 MTR_8g062440 39.262 298 149 11 22 306 40 318 8.47e-51 171
MS.gene026777.t1 MTR_4g077100 37.329 292 163 8 22 306 43 321 1.20e-50 171
MS.gene026777.t1 MTR_2g028620 41.772 237 127 3 29 263 49 276 9.87e-50 168
MS.gene026777.t1 MTR_8g062440 38.384 297 152 10 26 310 44 321 9.92e-50 168
MS.gene026777.t1 MTR_5g056600 32.526 289 182 4 26 310 40 319 1.14e-48 165
MS.gene026777.t1 MTR_8g062110 37.710 297 154 9 22 306 41 318 1.56e-48 165
MS.gene026777.t1 MTR_8g062110 37.710 297 154 9 22 306 50 327 1.63e-48 165
MS.gene026777.t1 MTR_4g077130 37.884 293 152 9 26 306 45 319 2.13e-48 164
MS.gene026777.t1 MTR_3g005170 37.295 244 138 5 26 266 46 277 1.97e-45 155
MS.gene026777.t1 MTR_3g031650 36.017 236 142 1 28 263 43 269 2.57e-45 155
MS.gene026777.t1 MTR_4g077100 37.903 248 139 6 22 266 43 278 8.43e-45 154
MS.gene026777.t1 MTR_1g022440 33.898 295 172 10 26 310 44 325 1.61e-44 155
MS.gene026777.t1 MTR_8g062440 38.697 261 137 9 26 283 44 284 5.40e-43 150
MS.gene026777.t1 MTR_8g062110 37.023 262 142 8 22 280 41 282 9.73e-43 149
MS.gene026777.t1 MTR_8g062110 37.603 242 135 6 22 260 41 269 7.15e-42 146
MS.gene026777.t1 MTR_1g022445 32.542 295 176 9 26 310 44 325 7.70e-42 148
MS.gene026777.t1 MTR_3g005170 33.101 287 143 7 26 306 46 289 3.74e-39 140
MS.gene026777.t1 MTR_4g092080 32.308 325 186 11 4 309 28 337 3.41e-37 135
MS.gene026777.t1 MTR_7g074880 31.724 290 177 6 30 310 49 326 1.98e-34 128
MS.gene026777.t1 MTR_8g062110 36.245 229 125 7 90 306 13 232 3.35e-33 122
MS.gene026777.t1 MTR_7g074820 33.220 295 169 10 30 310 49 329 7.67e-33 124
MS.gene026777.t1 MTR_7g074730 30.952 294 178 8 29 310 48 328 1.80e-32 123
MS.gene026777.t1 MTR_4g081440 31.683 303 181 11 29 312 56 351 5.01e-32 122
MS.gene026777.t1 MTR_7g074870 31.724 290 177 7 30 310 11 288 1.69e-31 119
MS.gene026777.t1 MTR_8g062710 93.103 58 4 0 22 79 41 98 3.21e-31 114
MS.gene026777.t1 MTR_4g081440 31.229 301 186 10 29 312 60 356 6.59e-31 119
MS.gene026777.t1 MTR_7g074850 30.612 294 178 10 30 310 49 329 5.74e-28 110
MS.gene026777.t1 MTR_8g062708 82.540 63 11 0 205 267 5 67 7.96e-28 104
MS.gene026777.t1 MTR_5g029990 31.365 271 140 8 37 290 55 296 6.14e-27 107
MS.gene026777.t1 MTR_7g074710 31.126 302 163 13 30 310 49 326 6.33e-27 108
MS.gene026777.t1 MTR_8g062110 35.632 174 106 4 90 260 13 183 1.07e-26 104
MS.gene026777.t1 MTR_4g023730 32.450 302 163 12 29 310 49 329 1.67e-24 101
MS.gene026777.t1 MTR_6g406250 30.736 231 142 7 31 255 95 313 1.09e-21 94.4
MS.gene026777.t1 MTR_0685s0030 34.356 163 93 4 152 306 2 158 3.68e-19 83.2
MS.gene026777.t1 MTR_0164s0070 44.444 99 45 2 26 123 41 130 6.27e-18 81.3
MS.gene026777.t1 MTR_6g406250 32.500 160 95 5 31 186 95 245 3.80e-17 80.1
MS.gene026777.t1 MTR_2g013230 25.868 317 183 12 26 310 44 340 7.08e-15 74.7
MS.gene026777.t1 MTR_3g011130 36.607 112 66 4 96 203 35 145 3.43e-14 69.3
MS.gene026777.t1 MTR_0685s0030 33.621 116 71 2 152 267 2 111 3.02e-13 65.9
MS.gene026777.t1 MTR_7g117190 26.721 247 145 11 30 252 46 280 2.20e-11 63.9
MS.gene026777.t1 MTR_7g117190 26.721 247 145 11 30 252 46 280 2.80e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene026777.t1 AT2G33590 61.538 286 100 2 26 310 44 320 1.25e-115 337
MS.gene026777.t1 AT2G33600 59.862 289 106 2 23 310 41 320 1.98e-107 316
MS.gene026777.t1 AT1G76470 50.000 290 131 3 26 310 42 322 4.99e-83 254
MS.gene026777.t1 AT1G80820 40.000 290 156 2 25 310 42 317 1.56e-60 196
MS.gene026777.t1 AT1G51410 40.956 293 153 7 26 311 45 324 8.15e-60 194
MS.gene026777.t1 AT1G09510 42.955 291 148 7 26 310 44 322 2.63e-58 190
MS.gene026777.t1 AT5G58490 38.462 286 164 2 28 310 47 323 3.48e-58 190
MS.gene026777.t1 AT5G19440 39.041 292 160 6 26 311 46 325 1.42e-56 186
MS.gene026777.t1 AT1G15950 39.310 290 158 2 25 310 47 322 1.47e-56 186
MS.gene026777.t1 AT1G15950 39.310 290 158 2 25 310 47 322 1.47e-56 186
MS.gene026777.t1 AT2G02400 35.540 287 169 3 29 310 43 318 7.10e-56 184
MS.gene026777.t1 AT1G80820 42.798 243 125 1 25 267 42 270 8.64e-54 177
MS.gene026777.t1 AT1G15950 38.929 280 153 2 25 300 47 312 2.35e-53 178
MS.gene026777.t1 AT1G15950 41.935 248 130 1 25 272 47 280 6.07e-53 175
MS.gene026777.t1 AT1G15950 42.449 245 127 1 25 269 47 277 7.63e-53 175
MS.gene026777.t1 AT1G15950 42.387 243 126 1 25 267 47 275 9.04e-52 172
MS.gene026777.t1 AT1G09490 36.552 290 168 6 26 310 13 291 1.09e-50 169
MS.gene026777.t1 AT1G09480 37.288 295 169 5 21 310 39 322 1.15e-50 171
MS.gene026777.t1 AT1G09480 37.288 295 169 5 21 310 86 369 2.85e-50 171
MS.gene026777.t1 AT1G09490 36.552 290 168 6 26 310 44 322 5.23e-50 169
MS.gene026777.t1 AT1G66800 40.206 291 152 8 26 310 44 318 1.58e-48 165
MS.gene026777.t1 AT4G35420 34.122 296 170 7 26 312 44 323 2.29e-48 164
MS.gene026777.t1 AT1G09500 37.329 292 166 6 26 310 10 291 2.79e-47 160
MS.gene026777.t1 AT1G09500 37.329 292 166 6 26 310 44 325 1.46e-46 160
MS.gene026777.t1 AT1G09500 41.485 229 122 4 26 252 44 262 1.19e-44 154
MS.gene026777.t1 AT1G15950 38.839 224 133 1 91 310 1 224 6.90e-41 143
MS.gene026777.t1 AT1G68540 31.271 291 185 5 26 310 40 321 1.30e-40 144
MS.gene026777.t1 AT5G42800 33.898 295 172 9 26 310 44 325 2.54e-40 145
MS.gene026777.t1 AT1G25460 30.928 291 185 4 26 310 39 319 5.17e-40 142
MS.gene026777.t1 AT2G23910 34.222 225 131 5 28 251 14 222 1.01e-33 125
MS.gene026777.t1 AT2G23910 34.222 225 131 5 28 251 48 256 1.54e-33 124
MS.gene026777.t1 AT2G23910 34.222 225 131 5 28 251 48 256 1.77e-33 124
MS.gene026777.t1 AT2G23910 34.222 225 131 5 28 251 48 256 2.17e-33 125
MS.gene026777.t1 AT2G45400 31.773 299 172 10 29 310 24 307 8.32e-33 123
MS.gene026777.t1 AT2G45400 30.769 325 192 11 4 310 55 364 2.59e-32 123
MS.gene026777.t1 AT4G30470 33.488 215 125 4 38 251 58 255 1.25e-30 117
MS.gene026777.t1 AT1G61720 29.429 333 193 12 4 313 27 340 3.41e-30 117
MS.gene026777.t1 AT4G27250 32.131 305 178 10 12 299 32 324 8.57e-30 116
MS.gene026777.t1 AT1G68540 31.401 207 138 2 108 310 8 214 4.23e-29 111
MS.gene026777.t1 AT4G27250 32.154 311 180 10 12 299 32 334 8.49e-29 114
MS.gene026777.t1 AT2G45400 31.273 275 159 9 4 266 55 311 8.37e-27 107
MS.gene026777.t1 AT5G14700 33.480 227 129 8 37 255 105 317 4.10e-21 92.8
MS.gene026777.t1 AT2G45400 33.173 208 121 6 4 203 55 252 6.75e-21 90.5
MS.gene026777.t1 AT5G14700 32.353 170 100 5 37 201 105 264 9.98e-16 76.6

Find 47 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTGTCCTACTCTTGTTT+TGG 0.178833 4.1:+85145758 MS.gene026777:CDS
TCATCAATCGCTTTATCTTT+TGG 0.244785 4.1:-85145438 None:intergenic
CTCAGAGAAACTGCAAAGTT+TGG 0.267800 4.1:+85147109 MS.gene026777:CDS
TTTAGCAAGTGTTCATATTT+CGG 0.276272 4.1:-85144962 None:intergenic
CGTAGTTGATGCAATACTTT+TGG 0.300274 4.1:+85146421 MS.gene026777:CDS
ATATGAACACTTGCTAAAAC+TGG 0.332196 4.1:+85144967 MS.gene026777:CDS
TTAGCAGTTCCCTTCACTTC+AGG 0.334105 4.1:-85145330 None:intergenic
CTGTTCTTCTGCTTCTGTCT+TGG 0.343397 4.1:-85145694 None:intergenic
CACTATCAACACACGAGATT+TGG 0.344680 4.1:+85146490 MS.gene026777:CDS
AAAGTTTGGGTTGGAAATTC+AGG 0.345192 4.1:+85147123 MS.gene026777:CDS
ACTCTTCAAAGCAGATATCT+TGG 0.353367 4.1:+85145012 MS.gene026777:CDS
CAAGACAGAAGCAGAAGAAC+AGG 0.371007 4.1:+85145695 MS.gene026777:CDS
AACCAAGCTACTTGCATTTG+TGG 0.373321 4.1:-85145799 None:intergenic
TTTGTGGTTGACTGTAAAAT+TGG 0.374021 4.1:-85145783 None:intergenic
TCAGTTTACTCTGCAATTGT+TGG 0.377727 4.1:+85145043 MS.gene026777:CDS
CTGACTTTCGCGAAAAGAAC+TGG 0.387817 4.1:+85145720 MS.gene026777:CDS
GTTAGAAGGCTGCGATTCAT+TGG 0.405647 4.1:+85146364 MS.gene026777:intron
TCAGAGAAACTGCAAAGTTT+GGG 0.410030 4.1:+85147110 MS.gene026777:CDS
GAAAATGAAGCTTACTTCGT+AGG 0.425779 4.1:-85146551 None:intergenic
CATTGGAGAATAAGCTTCGT+TGG 0.446781 4.1:+85146381 MS.gene026777:CDS
AAGCGATTGATGAGTCTTGT+TGG 0.452091 4.1:+85145448 MS.gene026777:CDS
GTCTGCCTTGTGATTCTCAT+AGG 0.459100 4.1:-85146444 None:intergenic
TATCTGCTTTGAAGAGTGTA+AGG 0.460836 4.1:-85145006 None:intergenic
GGTACAACTGTTGAAGGTAC+AGG 0.487565 4.1:-85145094 None:intergenic
AGAAACGTTACCTCAGGGTT+AGG 0.503091 4.1:-85145115 None:intergenic
AAAAGGTCGTATACAGGTCA+TGG 0.503870 4.1:-85144662 None:intergenic
GTTGAGAGCTATAAAGAAGC+TGG 0.507886 4.1:+85147173 MS.gene026777:CDS
GGGTTAGGTACAACTGTTGA+AGG 0.511645 4.1:-85145100 None:intergenic
AAAATGAAGCTTACTTCGTA+GGG 0.514933 4.1:-85146550 None:intergenic
AGAAACTGCAAAGTTTGGGT+TGG 0.515747 4.1:+85147114 MS.gene026777:CDS
TATCAACACACGAGATTTGG+TGG 0.518864 4.1:+85146493 MS.gene026777:CDS
GGGTTGGAAATTCAGGCCAT+TGG 0.519755 4.1:+85147130 MS.gene026777:CDS
TTGGAAATTCAGGCCATTGG+AGG 0.526899 4.1:+85147133 MS.gene026777:CDS
TCAATGAGTGTTTCCTCCAA+TGG 0.543548 4.1:-85147146 None:intergenic
AACCACAAATGCAAGTAGCT+TGG 0.550767 4.1:+85145797 MS.gene026777:CDS
ATGAGAATCACAAGGCAGAC+GGG 0.568344 4.1:+85146447 MS.gene026777:CDS
ATTGGTCCCAAAACAAGAGT+AGG 0.572490 4.1:-85145765 None:intergenic
TATGAGAATCACAAGGCAGA+CGG 0.581333 4.1:+85146446 MS.gene026777:CDS
AAGGTACAGGGCTAGCAACA+TGG 0.584108 4.1:-85145081 None:intergenic
AGTGATTGAGCCTGAAGTGA+AGG 0.601324 4.1:+85145320 MS.gene026777:CDS
GTGATTGAGCCTGAAGTGAA+GGG 0.607911 4.1:+85145321 MS.gene026777:CDS
TGCTGGCAGCTATATTGAGA+TGG 0.610431 4.1:+85147067 MS.gene026777:intron
GTACAACTGTTGAAGGTACA+GGG 0.621515 4.1:-85145093 None:intergenic
AGCTTGTCTCAAAGCTAATG+TGG 0.639403 4.1:+85145362 MS.gene026777:CDS
GAAAAGAACTGGACTTAACG+TGG 0.670351 4.1:+85145731 MS.gene026777:CDS
TTTGGCCTATGAGAATCACA+AGG 0.727463 4.1:+85146439 MS.gene026777:CDS
AACAGTTGTACCTAACCCTG+AGG 0.743171 4.1:+85145105 MS.gene026777:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTGATAATATTTAAATTTAA+AGG - chr4.1:85146599-85146618 None:intergenic 10.0%
!! TAATATTTAAATTTAAAGGT+TGG - chr4.1:85146595-85146614 None:intergenic 10.0%
!! AAGTCAATAATTAATTAATG+AGG + chr4.1:85146688-85146707 MS.gene026777:intron 15.0%
!! TGAAATTAGTTAAATTATAC+TGG - chr4.1:85146285-85146304 None:intergenic 15.0%
!! TTAATGAGGAATATATGTAA+AGG + chr4.1:85146702-85146721 MS.gene026777:intron 20.0%
!!! AAAGAAAGAATTTTTTGCAA+CGG + chr4.1:85146655-85146674 MS.gene026777:intron 20.0%
!!! ATTTGATAAAACAGAAGTAT+TGG - chr4.1:85146939-85146958 None:intergenic 20.0%
!!! CTGTTTTATCAAATTATCAT+TGG + chr4.1:85146945-85146964 MS.gene026777:intron 20.0%
!!! CTTTATCTTTTGGTAAATTA+GGG - chr4.1:85145431-85145450 None:intergenic 20.0%
! AATTGTATCAGGAAAATTGT+AGG + chr4.1:85146758-85146777 MS.gene026777:intron 25.0%
! ATTGTATCAGGAAAATTGTA+GGG + chr4.1:85146759-85146778 MS.gene026777:intron 25.0%
! CACATATAAAGGCTAAAAAA+TGG - chr4.1:85146117-85146136 None:intergenic 25.0%
! CATAACTTCTATAAAATCCT+TGG + chr4.1:85145945-85145964 MS.gene026777:intron 25.0%
! GTTGTTATAAGAACTTGATA+AGG - chr4.1:85145859-85145878 None:intergenic 25.0%
! TGTAACCATCAAATCTATAT+AGG - chr4.1:85147026-85147045 None:intergenic 25.0%
! TTATGTGGTTATCATTATCA+TGG - chr4.1:85145930-85145949 None:intergenic 25.0%
! TTTAGCAAGTGTTCATATTT+CGG - chr4.1:85144965-85144984 None:intergenic 25.0%
!! AGGATTTTATAGAAGTTATG+TGG - chr4.1:85145945-85145964 None:intergenic 25.0%
!! GCTTTATCTTTTGGTAAATT+AGG - chr4.1:85145432-85145451 None:intergenic 25.0%
!! TATGTTAAAGTTTTCATTGC+TGG + chr4.1:85147050-85147069 MS.gene026777:intron 25.0%
!! TTCAATCATCGAAGAATTTT+GGG + chr4.1:85145619-85145638 MS.gene026777:intron 25.0%
!! TTTCAATCATCGAAGAATTT+TGG + chr4.1:85145618-85145637 MS.gene026777:intron 25.0%
!! TTTTGGCGTCAATATTTATT+TGG + chr4.1:85146016-85146035 MS.gene026777:intron 25.0%
!!! ACTTTGAAGAAAATTTTGAC+TGG + chr4.1:85144709-85144728 MS.gene026777:CDS 25.0%
!!! CGAAGCAAAGAATTTTTTTT+CGG + chr4.1:85144831-85144850 MS.gene026777:intron 25.0%
AAAATGAAGCTTACTTCGTA+GGG - chr4.1:85146553-85146572 None:intergenic 30.0%
AAGTTATGCAGAGAATAATC+CGG - chr4.1:85146254-85146273 None:intergenic 30.0%
ACTTGCCTATATAGATTTGA+TGG + chr4.1:85147018-85147037 MS.gene026777:intron 30.0%
AGACTACTGCAAAAATACTA+AGG + chr4.1:85145479-85145498 MS.gene026777:CDS 30.0%
ATGAAAATCTCTCAACTTAC+TGG - chr4.1:85145215-85145234 None:intergenic 30.0%
CATTATCATGGAAAACAACA+AGG - chr4.1:85145918-85145937 None:intergenic 30.0%
GACACTACTATCATATCATA+AGG - chr4.1:85145552-85145571 None:intergenic 30.0%
GACTTACCTTAAACACTTAT+TGG - chr4.1:85144812-85144831 None:intergenic 30.0%
GCATCTCAGATATATTTACA+GGG - chr4.1:85144787-85144806 None:intergenic 30.0%
GTATGGACATACACATATAA+AGG - chr4.1:85146128-85146147 None:intergenic 30.0%
TCAACATTCATACGACTATT+TGG - chr4.1:85144746-85144765 None:intergenic 30.0%
TTGTGATCAACAATTGTATC+AGG + chr4.1:85146747-85146766 MS.gene026777:intron 30.0%
TTTGTGGTTGACTGTAAAAT+TGG - chr4.1:85145786-85145805 None:intergenic 30.0%
! ACATTACTTGCAATTTTTCC+CGG - chr4.1:85146798-85146817 None:intergenic 30.0%
! ATATGAACACTTGCTAAAAC+TGG + chr4.1:85144967-85144986 MS.gene026777:CDS 30.0%
! GTTCTCGTCAAACTTTTAAA+AGG + chr4.1:85145819-85145838 MS.gene026777:CDS 30.0%
! TAGCAGATAGTTTAGCTTAA+CGG - chr4.1:85146199-85146218 None:intergenic 30.0%
! TCATCAATCGCTTTATCTTT+TGG - chr4.1:85145441-85145460 None:intergenic 30.0%
!! TTTTTGAAAAGGTCGTATAC+AGG - chr4.1:85144671-85144690 None:intergenic 30.0%
!!! AAGCTTCAAGTACTTTTTCA+TGG - chr4.1:85145286-85145305 None:intergenic 30.0%
!!! ATTTGTCCTACTCTTGTTTT+GGG + chr4.1:85145759-85145778 MS.gene026777:CDS 30.0%
AACAATTTCGATGACACTGA+TGG - chr4.1:85145243-85145262 None:intergenic 35.0%
AACACTTGTCGTATGAATGT+TGG + chr4.1:85144854-85144873 MS.gene026777:intron 35.0%
ACTCTTCAAAGCAGATATCT+TGG + chr4.1:85145012-85145031 MS.gene026777:CDS 35.0%
ACTTGATAAGGCATATCAGT+AGG - chr4.1:85145847-85145866 None:intergenic 35.0%
AGTTATGCAGAGAATAATCC+GGG - chr4.1:85146253-85146272 None:intergenic 35.0%
ATCATCCTGTCTTTGTTAGA+AGG + chr4.1:85146350-85146369 MS.gene026777:intron 35.0%
ATGCGTCCAATAAGTGTTTA+AGG + chr4.1:85144803-85144822 MS.gene026777:intron 35.0%
ATTGTCGCATCGAAAAATTC+AGG + chr4.1:85146858-85146877 MS.gene026777:intron 35.0%
CGCATCTCAGATATATTTAC+AGG - chr4.1:85144788-85144807 None:intergenic 35.0%
GAAAATGAAGCTTACTTCGT+AGG - chr4.1:85146554-85146573 None:intergenic 35.0%
TCAGAGAAACTGCAAAGTTT+GGG + chr4.1:85147110-85147129 MS.gene026777:CDS 35.0%
TCAGTTTACTCTGCAATTGT+TGG + chr4.1:85145043-85145062 MS.gene026777:CDS 35.0%
TCATGAATTACGAATATGCC+CGG + chr4.1:85146232-85146251 MS.gene026777:intron 35.0%
TGTCAATATCGTCTTAATGC+CGG - chr4.1:85146903-85146922 None:intergenic 35.0%
! GCATAACAGAATCAGAGTTT+TGG + chr4.1:85145999-85146018 MS.gene026777:intron 35.0%
!! AAAGTTTGGGTTGGAAATTC+AGG + chr4.1:85147123-85147142 MS.gene026777:CDS 35.0%
!! CATCGAAAAATTCAGGTTGA+AGG + chr4.1:85146865-85146884 MS.gene026777:intron 35.0%
!! CATTTGTCCTACTCTTGTTT+TGG + chr4.1:85145758-85145777 MS.gene026777:CDS 35.0%
!! CGTAGTTGATGCAATACTTT+TGG + chr4.1:85146421-85146440 MS.gene026777:CDS 35.0%
!! TATCTGCTTTGAAGAGTGTA+AGG - chr4.1:85145009-85145028 None:intergenic 35.0%
!! TGCTGAGCAGATTTTTGAAA+AGG - chr4.1:85144682-85144701 None:intergenic 35.0%
AACCAAGCTACTTGCATTTG+TGG - chr4.1:85145802-85145821 None:intergenic 40.0%
AACCACAAATGCAAGTAGCT+TGG + chr4.1:85145797-85145816 MS.gene026777:CDS 40.0%
AACGTTAGAAACGTTACCTC+AGG - chr4.1:85145124-85145143 None:intergenic 40.0%
ACGTTAGAAACGTTACCTCA+GGG - chr4.1:85145123-85145142 None:intergenic 40.0%
AGAAACTGCAAAGTTTGGGT+TGG + chr4.1:85147114-85147133 MS.gene026777:CDS 40.0%
AGCTTGTCTCAAAGCTAATG+TGG + chr4.1:85145362-85145381 MS.gene026777:CDS 40.0%
ATTGGTCCCAAAACAAGAGT+AGG - chr4.1:85145768-85145787 None:intergenic 40.0%
CACTATCAACACACGAGATT+TGG + chr4.1:85146490-85146509 MS.gene026777:CDS 40.0%
CATTGGAGAATAAGCTTCGT+TGG + chr4.1:85146381-85146400 MS.gene026777:CDS 40.0%
CTCAGAGAAACTGCAAAGTT+TGG + chr4.1:85147109-85147128 MS.gene026777:CDS 40.0%
GAAAAGAACTGGACTTAACG+TGG + chr4.1:85145731-85145750 MS.gene026777:CDS 40.0%
GTACTTGAAGCTTAATGTGC+AGG + chr4.1:85145293-85145312 MS.gene026777:intron 40.0%
GTGTTCCAACAAAAATGCGT+TGG - chr4.1:85144883-85144902 None:intergenic 40.0%
GTTGAGAGCTATAAAGAAGC+TGG + chr4.1:85147173-85147192 MS.gene026777:CDS 40.0%
TAATTTGATATGTCACCGCG+TGG + chr4.1:85145159-85145178 MS.gene026777:intron 40.0%
TATCAACACACGAGATTTGG+TGG + chr4.1:85146493-85146512 MS.gene026777:CDS 40.0%
TATGAGAATCACAAGGCAGA+CGG + chr4.1:85146446-85146465 MS.gene026777:CDS 40.0%
TCAATGAGTGTTTCCTCCAA+TGG - chr4.1:85147149-85147168 None:intergenic 40.0%
TGGCATAACAATACCAGTTC+TGG - chr4.1:85145677-85145696 None:intergenic 40.0%
TTTGGCCTATGAGAATCACA+AGG + chr4.1:85146439-85146458 MS.gene026777:CDS 40.0%
! AAGCGATTGATGAGTCTTGT+TGG + chr4.1:85145448-85145467 MS.gene026777:CDS 40.0%
! AGTTCTTTTCGCGAAAGTCA+GGG - chr4.1:85145721-85145740 None:intergenic 40.0%
! CTTCACCGCTATAGTGTTTT+GGG + chr4.1:85146167-85146186 MS.gene026777:intron 40.0%
! GTACAACTGTTGAAGGTACA+GGG - chr4.1:85145096-85145115 None:intergenic 40.0%
!!! TGGTTCTAGTTTTGCCTTCA+TGG + chr4.1:85145179-85145198 MS.gene026777:intron 40.0%
AAACACTATAGCGGTGAAGC+AGG - chr4.1:85146166-85146185 None:intergenic 45.0%
AACAGTTGTACCTAACCCTG+AGG + chr4.1:85145105-85145124 MS.gene026777:CDS 45.0%
AAGGCAAAACTAGAACCACG+CGG - chr4.1:85145177-85145196 None:intergenic 45.0%
AGAAACGTTACCTCAGGGTT+AGG - chr4.1:85145118-85145137 None:intergenic 45.0%
AGTGATTGAGCCTGAAGTGA+AGG + chr4.1:85145320-85145339 MS.gene026777:CDS 45.0%
ATGAGAATCACAAGGCAGAC+GGG + chr4.1:85146447-85146466 MS.gene026777:CDS 45.0%
CAAGACAGAAGCAGAAGAAC+AGG + chr4.1:85145695-85145714 MS.gene026777:CDS 45.0%
CGACTCCCAAAACACTATAG+CGG - chr4.1:85146175-85146194 None:intergenic 45.0%
CTGACTTTCGCGAAAAGAAC+TGG + chr4.1:85145720-85145739 MS.gene026777:CDS 45.0%
CTGTTCTTCTGCTTCTGTCT+TGG - chr4.1:85145697-85145716 None:intergenic 45.0%
CTTGAAGCTTAATGTGCAGG+TGG + chr4.1:85145296-85145315 MS.gene026777:intron 45.0%
GGGTTAGGTACAACTGTTGA+AGG - chr4.1:85145103-85145122 None:intergenic 45.0%
GTCTGCCTTGTGATTCTCAT+AGG - chr4.1:85146447-85146466 None:intergenic 45.0%
GTGATTGAGCCTGAAGTGAA+GGG + chr4.1:85145321-85145340 MS.gene026777:CDS 45.0%
GTTAGAAGGCTGCGATTCAT+TGG + chr4.1:85146364-85146383 MS.gene026777:intron 45.0%
TAGCATGTCAGTGTCGTGTT+CGG + chr4.1:85146971-85146990 MS.gene026777:intron 45.0%
TGCTGGCAGCTATATTGAGA+TGG + chr4.1:85147067-85147086 MS.gene026777:intron 45.0%
TTAGCAGTTCCCTTCACTTC+AGG - chr4.1:85145333-85145352 None:intergenic 45.0%
TTGCAAGTAATGTTGCGCGA+CGG + chr4.1:85146805-85146824 MS.gene026777:intron 45.0%
TTGGAAATTCAGGCCATTGG+AGG + chr4.1:85147133-85147152 MS.gene026777:CDS 45.0%
! CAGTTCTTTTCGCGAAAGTC+AGG - chr4.1:85145722-85145741 None:intergenic 45.0%
! CTGGTGTGTTTTCACCATGA+AGG - chr4.1:85145196-85145215 None:intergenic 45.0%
! GCTTCACCGCTATAGTGTTT+TGG + chr4.1:85146166-85146185 MS.gene026777:intron 45.0%
! GGACACCAACGCATTTTTGT+TGG + chr4.1:85144875-85144894 MS.gene026777:intron 45.0%
! GGTACAACTGTTGAAGGTAC+AGG - chr4.1:85145097-85145116 None:intergenic 45.0%
! GGTAGTGGTAGTATGGTGTA+TGG - chr4.1:85146145-85146164 None:intergenic 45.0%
! TGAAGCAGGTAGTGGTAGTA+TGG - chr4.1:85146152-85146171 None:intergenic 45.0%
! TTGAAGGTGTGTCTTGTGTC+CGG + chr4.1:85146881-85146900 MS.gene026777:intron 45.0%
!!! CGCTATAGTGTTTTGGGAGT+CGG + chr4.1:85146173-85146192 MS.gene026777:intron 45.0%
ACGCTACTGTTACACAGCCA+AGG - chr4.1:85145965-85145984 None:intergenic 50.0%
CGCAGCCTTCTAACAAAGAC+AGG - chr4.1:85146358-85146377 None:intergenic 50.0%
CTTCGCATGTGATCCAGAAC+TGG + chr4.1:85145661-85145680 MS.gene026777:intron 50.0%
GGGTTGGAAATTCAGGCCAT+TGG + chr4.1:85147130-85147149 MS.gene026777:CDS 50.0%
GTAGGGTCATGAGAATGAGC+CGG + chr4.1:85146776-85146795 MS.gene026777:intron 50.0%
TAGGGTCATGAGAATGAGCC+GGG + chr4.1:85146777-85146796 MS.gene026777:intron 50.0%
TATAGCGGTGAAGCAGGTAG+TGG - chr4.1:85146160-85146179 None:intergenic 50.0%
TTCGGACTACCTGCAGTTGT+CGG - chr4.1:85144947-85144966 None:intergenic 50.0%
! AAGGTACAGGGCTAGCAACA+TGG - chr4.1:85145084-85145103 None:intergenic 50.0%
AGTGCATGTCCGACAACTGC+AGG + chr4.1:85144935-85144954 MS.gene026777:intron 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 85144664 85147214 85144664 ID=MS.gene026777
chr4.1 mRNA 85144664 85147214 85144664 ID=MS.gene026777.t1;Parent=MS.gene026777
chr4.1 exon 85144664 85144730 85144664 ID=MS.gene026777.t1.exon1;Parent=MS.gene026777.t1
chr4.1 CDS 85144664 85144730 85144664 ID=cds.MS.gene026777.t1;Parent=MS.gene026777.t1
chr4.1 exon 85144957 85145126 85144957 ID=MS.gene026777.t1.exon2;Parent=MS.gene026777.t1
chr4.1 CDS 85144957 85145126 85144957 ID=cds.MS.gene026777.t1;Parent=MS.gene026777.t1
chr4.1 exon 85145188 85145214 85145188 ID=MS.gene026777.t1.exon3;Parent=MS.gene026777.t1
chr4.1 CDS 85145188 85145214 85145188 ID=cds.MS.gene026777.t1;Parent=MS.gene026777.t1
chr4.1 exon 85145315 85145500 85145315 ID=MS.gene026777.t1.exon4;Parent=MS.gene026777.t1
chr4.1 CDS 85145315 85145500 85145315 ID=cds.MS.gene026777.t1;Parent=MS.gene026777.t1
chr4.1 exon 85145678 85145840 85145678 ID=MS.gene026777.t1.exon5;Parent=MS.gene026777.t1
chr4.1 CDS 85145678 85145840 85145678 ID=cds.MS.gene026777.t1;Parent=MS.gene026777.t1
chr4.1 exon 85146369 85146558 85146369 ID=MS.gene026777.t1.exon6;Parent=MS.gene026777.t1
chr4.1 CDS 85146369 85146558 85146369 ID=cds.MS.gene026777.t1;Parent=MS.gene026777.t1
chr4.1 exon 85147076 85147214 85147076 ID=MS.gene026777.t1.exon7;Parent=MS.gene026777.t1
chr4.1 CDS 85147076 85147214 85147076 ID=cds.MS.gene026777.t1;Parent=MS.gene026777.t1
Gene Sequence

>MS.gene026777

ATGACCTGTATACGACCTTTTCAAAAATCTGCTCAGCAGTTGAAAACTTTGAAGAAAATTTTGACTGGTTAATAGCATTCCAAATAGTCGTATGAATGTTGAAGCAAAGAAATTTTTAATCCCTGTAAATATATCTGAGATGCGTCCAATAAGTGTTTAAGGTAAGTCGAAGCAAAGAATTTTTTTTCGGAACACTTGTCGTATGAATGTTGGACACCAACGCATTTTTGTTGGAACACTTGTCGTACGAGTGTTTTTGCGATTCATTTGAAGTGCATGTCCGACAACTGCAGGTAGTCCGAAATATGAACACTTGCTAAAACTGGAGAAAGCTTCTGAGAACCTTACACTCTTCAAAGCAGATATCTTGGATTACGAATCAGTTTACTCTGCAATTGTTGGATGCAGTGCAGTTTTCCATGTTGCTAGCCCTGTACCTTCAACAGTTGTACCTAACCCTGAGGTAACGTTTCTAACGTTCTTTTATATGCAGACTAATTTGATATGTCACCGCGTGGTTCTAGTTTTGCCTTCATGGTGAAAACACACCAGTAAGTTGAGAGATTTTCATCTTATCCATCAGTGTCATCGAAATTGTTCATGTTAGTAAACACGACATCCATGAAAAAGTACTTGAAGCTTAATGTGCAGGTGGAAGTGATTGAGCCTGAAGTGAAGGGAACTGCTAATGTACTTGAAGCTTGTCTCAAAGCTAATGTGGAACGTGTCGTCTTTGTATCATCTGCAGCTGCTGTTGCTATCAACCCTAATTTACCAAAAGATAAAGCGATTGATGAGTCTTGTTGGTCTGACAAAGACTACTGCAAAAATACTAAGGTCACTAAGATTTTATTATATTTGTGCATGACATAAATGTATCATTAGCCTTATGATATGATAGTAGTGTCTTATAATTTTTTTTCTTCAGATCTTTAAGTTTCAAAATATGTTATGTTTCAATCATCGAAGAATTTTGGGTTATGTAAATAAGCTTATACTTCGCATGTGATCCAGAACTGGTATTGTTATGCCAAGACAGAAGCAGAAGAACAGGCCCTGACTTTCGCGAAAAGAACTGGACTTAACGTGGTAAGCATTTGTCCTACTCTTGTTTTGGGACCAATTTTACAGTCAACCACAAATGCAAGTAGCTTGGTTCTCGTCAAACTTTTAAAAGGTACCTACTGATATGCCTTATCAAGTTCTTATAACAACATGCATATTTGTGAACTGTATAATCGAGTTAAAATTCCTTGTTGTTTTCCATGATAATGATAACCACATAACTTCTATAAAATCCTTGGCTGTGTAACAGTAGCGTTTTATTTTTAGTGTGCATAACAGAATCAGAGTTTTGGCGTCAATATTTATTTGGATATTATGTTTGAATATTAGTAGCAATTTTTTTTGATGATTAGTTATAAATATCTGTTAAGTTTTGTGTGTTCTTCCATTTTTTAGCCTTTATATGTGTATGTCCATACACCATACTACCACTACCTGCTTCACCGCTATAGTGTTTTGGGAGTCGGCCGTTAAGCTAAACTATCTGCTACTAAATAGATAGATCATGAATTACGAATATGCCCGGATTATTCTCTGCATAACTTGTTTTCTGCCAGTATAATTTAACTAATTTCATATACTATAGATTTCATATAAATGCTAAAATCATTTTTCATGTTAATCATCCTGTCTTTGTTAGAAGGCTGCGATTCATTGGAGAATAAGCTTCGTTGGATTGTTGACGTACAAGACGTAGTTGATGCAATACTTTTGGCCTATGAGAATCACAAGGCAGACGGGAGATACATCTGCACTTCACACACTATCAACACACGAGATTTGGTGGAGAAATCGAAGAGTATATATCTGAACTACAAGTACCCTACGAAGTAAGCTTCATTTTCTAAAGTAGTAGTCATAAACCAACCTTTAAATTTAAATATTATCAGTAATATTAATAATATCAAGATATATAAGAAATGTGAAAAGAAAGAATTTTTTGCAACGGTAAACTAAAGAAGTCAATAATTAATTAATGAGGAATATATGTAAAGGAGATGAACTATTAAGCGTGTTCTTGTGATCAACAATTGTATCAGGAAAATTGTAGGGTCATGAGAATGAGCCGGGAAAAATTGCAAGTAATGTTGCGCGACGGAGTACTTGCAGTGTCGATCTTAATAGTTGCATTGTCGCATCGAAAAATTCAGGTTGAAGGTGTGTCTTGTGTCCGGCATTAAGACGATATTGACACATGTGTTTAAATCCAATACTTCTGTTTTATCAAATTATCATTGGTGTTAGCATGTCAGTGTCGTGTTCGGTGTATGTGTCAATGTTTCATAGCTACTTGCCTATATAGATTTGATGGTTACAATCTTATGTTAAAGTTTTCATTGCTGGCAGCTATATTGAGATGGATGATTACAAAATGCTGAGCTCAGAGAAACTGCAAAGTTTGGGTTGGAAATTCAGGCCATTGGAGGAAACACTCATTGACTCTGTTGAGAGCTATAAAGAAGCTGGACTACTTCAATCAGAATAA
Protein sequence

>MS.gene026777.t1

MTCIRPFQKSAQQLKTLKKILTGSPKYEHLLKLEKASENLTLFKADILDYESVYSAIVGCSAVFHVASPVPSTVVPNPEFCLHGENTPVEVIEPEVKGTANVLEACLKANVERVVFVSSAAAVAINPNLPKDKAIDESCWSDKDYCKNTKNWYCYAKTEAEEQALTFAKRTGLNVVSICPTLVLGPILQSTTNASSLVLVKLLKEGCDSLENKLRWIVDVQDVVDAILLAYENHKADGRYICTSHTINTRDLVEKSKSIYLNYKYPTNYIEMDDYKMLSSEKLQSLGWKFRPLEETLIDSVESYKEAGLLQSE