AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053809

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053809.t1 MTR_5g056600 96.865 319 10 0 1 319 1 319 0.0 649
MS.gene053809.t1 MTR_3g005210 41.693 319 177 6 5 319 7 320 7.52e-86 261
MS.gene053809.t1 MTR_8g062440 42.006 319 175 6 5 319 9 321 6.27e-83 254
MS.gene053809.t1 MTR_3g005170 41.379 319 176 7 5 318 11 323 6.38e-83 254
MS.gene053809.t1 MTR_2g101330 42.271 317 178 4 5 319 6 319 2.16e-82 253
MS.gene053809.t1 MTR_8g062110 43.038 316 170 6 5 316 10 319 3.83e-82 251
MS.gene053809.t1 MTR_8g062110 43.038 316 170 6 5 316 19 328 5.45e-82 251
MS.gene053809.t1 MTR_8g062440 41.875 320 175 7 5 319 9 322 5.89e-81 249
MS.gene053809.t1 MTR_3g005170 45.149 268 137 6 5 267 11 273 2.24e-78 240
MS.gene053809.t1 MTR_8g062440 43.390 295 158 5 5 295 9 298 1.05e-76 238
MS.gene053809.t1 MTR_8g062110 46.442 267 134 5 5 267 10 271 1.56e-76 236
MS.gene053809.t1 MTR_8g062110 46.442 267 134 5 5 267 10 271 2.45e-76 236
MS.gene053809.t1 MTR_2g104960 42.271 317 175 5 4 319 20 329 3.58e-75 234
MS.gene053809.t1 MTR_4g006940 42.089 316 175 5 5 319 18 326 7.04e-74 231
MS.gene053809.t1 MTR_1g022445 38.629 321 187 7 5 319 9 325 2.59e-72 227
MS.gene053809.t1 MTR_4g077100 40.063 317 183 6 5 318 12 324 3.13e-71 224
MS.gene053809.t1 MTR_4g077130 39.557 316 186 5 5 318 10 322 3.15e-71 224
MS.gene053809.t1 MTR_1g022440 37.812 320 191 6 5 319 9 325 9.48e-71 223
MS.gene053809.t1 MTR_5g072620 38.291 316 192 3 5 319 6 319 1.69e-69 219
MS.gene053809.t1 MTR_4g009040 36.760 321 194 5 3 319 12 327 6.75e-68 215
MS.gene053809.t1 MTR_4g009040 36.562 320 192 5 3 319 12 323 4.62e-67 213
MS.gene053809.t1 MTR_3g005170 36.164 318 163 6 5 318 11 292 1.02e-66 211
MS.gene053809.t1 MTR_4g006940 44.238 269 144 3 5 273 18 280 4.82e-66 209
MS.gene053809.t1 MTR_4g006940 44.238 269 144 3 5 273 18 280 4.82e-66 209
MS.gene053809.t1 MTR_4g077100 42.105 266 148 5 5 267 12 274 3.92e-65 206
MS.gene053809.t1 MTR_7g074880 35.093 322 198 7 5 319 9 326 2.37e-63 203
MS.gene053809.t1 MTR_3g031650 33.544 316 207 3 5 319 6 319 4.45e-63 202
MS.gene053809.t1 MTR_3g031650 36.364 264 168 0 5 268 6 269 1.60e-60 194
MS.gene053809.t1 MTR_7g074730 36.111 324 194 8 5 319 9 328 5.89e-60 195
MS.gene053809.t1 MTR_4g081440 37.202 336 188 8 3 319 23 354 7.32e-60 196
MS.gene053809.t1 MTR_4g081440 36.905 336 188 8 3 319 19 349 1.75e-59 194
MS.gene053809.t1 MTR_7g074820 36.923 325 191 8 5 319 9 329 2.04e-59 193
MS.gene053809.t1 MTR_4g009690 35.312 320 196 5 3 319 12 323 3.98e-59 192
MS.gene053809.t1 MTR_4g009690 35.625 320 194 6 3 319 12 322 5.58e-59 192
MS.gene053809.t1 MTR_7g074850 33.846 325 201 8 5 319 9 329 1.07e-53 179
MS.gene053809.t1 MTR_7g074710 33.639 327 196 7 5 319 9 326 7.75e-53 176
MS.gene053809.t1 MTR_4g092080 31.707 328 203 9 5 317 15 336 2.05e-52 176
MS.gene053809.t1 MTR_8g062110 37.719 228 133 5 93 316 11 233 1.59e-46 157
MS.gene053809.t1 MTR_2g028620 30.938 320 212 4 5 319 11 326 1.23e-45 158
MS.gene053809.t1 MTR_4g023730 31.481 324 210 7 5 319 9 329 2.60e-44 154
MS.gene053809.t1 MTR_2g028620 33.704 270 171 3 5 269 11 277 3.00e-44 154
MS.gene053809.t1 MTR_7g074870 32.069 290 187 6 36 319 3 288 4.58e-44 152
MS.gene053809.t1 MTR_8g062110 41.341 179 97 4 93 267 11 185 7.56e-41 141
MS.gene053809.t1 MTR_2g013250 28.190 337 211 8 5 317 8 337 3.56e-33 125
MS.gene053809.t1 MTR_2g013230 29.032 341 207 10 5 319 9 340 3.98e-32 122
MS.gene053809.t1 MTR_6g406250 31.541 279 174 9 5 274 56 326 9.62e-31 119
MS.gene053809.t1 MTR_7g117190 28.402 338 214 12 5 319 8 340 1.56e-29 115
MS.gene053809.t1 MTR_2g013260 27.536 345 203 9 5 317 9 338 7.36e-29 114
MS.gene053809.t1 MTR_5g029990 26.400 250 158 7 5 247 13 243 4.25e-28 110
MS.gene053809.t1 MTR_0164s0070 43.750 128 71 1 3 129 4 131 2.43e-27 106
MS.gene053809.t1 MTR_7g117190 29.123 285 180 10 5 268 8 291 1.17e-26 107
MS.gene053809.t1 MTR_6g406250 32.701 211 132 6 5 208 56 263 1.76e-26 105
MS.gene053809.t1 MTR_0685s0030 34.356 163 105 2 156 318 1 161 2.54e-26 102
MS.gene053809.t1 MTR_2g013260 28.041 296 168 8 5 268 9 291 1.73e-25 103
MS.gene053809.t1 MTR_0685s0030 36.607 112 70 1 156 267 1 111 3.65e-20 84.7
MS.gene053809.t1 MTR_3g011130 39.655 116 67 3 96 208 30 145 1.09e-19 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053809.t1 AT1G68540 73.520 321 83 1 1 319 1 321 0.0 504
MS.gene053809.t1 AT1G25460 64.486 321 110 3 1 319 1 319 1.12e-151 428
MS.gene053809.t1 AT1G68540 73.832 214 54 1 108 319 1 214 6.24e-120 343
MS.gene053809.t1 AT4G35420 47.950 317 159 5 5 319 9 321 2.15e-98 293
MS.gene053809.t1 AT1G51410 42.138 318 177 6 5 319 10 323 1.44e-84 258
MS.gene053809.t1 AT5G19440 41.772 316 179 5 5 318 11 323 9.52e-82 251
MS.gene053809.t1 AT1G09510 40.764 314 181 5 5 316 9 319 6.94e-74 230
MS.gene053809.t1 AT5G58490 36.677 319 193 4 5 319 10 323 1.03e-67 215
MS.gene053809.t1 AT1G15950 39.873 316 182 5 5 319 14 322 2.58e-67 214
MS.gene053809.t1 AT1G15950 39.873 316 182 5 5 319 14 322 2.58e-67 214
MS.gene053809.t1 AT5G42800 38.199 322 187 8 5 319 9 325 2.41e-66 213
MS.gene053809.t1 AT1G09480 37.224 317 194 5 5 319 9 322 3.20e-66 211
MS.gene053809.t1 AT1G09480 37.224 317 194 5 5 319 56 369 1.04e-65 211
MS.gene053809.t1 AT1G09490 36.991 319 192 6 5 319 9 322 1.88e-65 209
MS.gene053809.t1 AT1G80820 38.924 316 185 4 5 319 9 317 4.28e-65 208
MS.gene053809.t1 AT1G09500 35.714 322 189 6 5 316 9 322 7.96e-64 205
MS.gene053809.t1 AT1G66800 37.730 326 176 9 5 319 9 318 6.65e-63 202
MS.gene053809.t1 AT1G15950 39.145 304 178 4 5 307 14 311 6.80e-63 202
MS.gene053809.t1 AT1G09500 39.098 266 155 4 5 266 9 271 1.98e-61 197
MS.gene053809.t1 AT1G15950 41.264 269 152 3 5 273 14 276 2.33e-59 192
MS.gene053809.t1 AT2G02400 31.861 317 209 5 5 319 7 318 3.72e-59 192
MS.gene053809.t1 AT1G15950 41.509 265 149 3 5 269 14 272 4.79e-59 191
MS.gene053809.t1 AT1G15950 41.509 265 149 3 5 269 14 272 8.61e-59 191
MS.gene053809.t1 AT2G33590 36.013 311 193 4 5 314 10 315 7.61e-57 187
MS.gene053809.t1 AT1G80820 39.405 269 157 2 5 273 9 271 9.53e-56 182
MS.gene053809.t1 AT1G09490 35.069 288 178 6 36 319 9 291 1.22e-51 172
MS.gene053809.t1 AT1G09500 34.130 293 175 6 34 316 4 288 1.78e-51 172
MS.gene053809.t1 AT4G27250 33.234 334 187 7 4 310 13 337 2.36e-51 173
MS.gene053809.t1 AT4G27250 33.436 326 187 7 4 310 13 327 2.75e-51 173
MS.gene053809.t1 AT1G61720 33.535 331 197 7 5 319 14 337 7.38e-51 172
MS.gene053809.t1 AT2G33600 35.443 316 192 6 3 314 8 315 3.52e-50 169
MS.gene053809.t1 AT2G45400 32.822 326 206 8 5 319 41 364 1.84e-47 163
MS.gene053809.t1 AT1G76470 34.906 318 201 4 5 319 8 322 1.20e-46 160
MS.gene053809.t1 AT2G45400 34.307 274 169 6 5 268 41 313 8.07e-44 153
MS.gene053809.t1 AT1G15950 35.556 225 143 2 96 319 1 224 6.28e-40 140
MS.gene053809.t1 AT2G45400 30.903 288 188 7 41 319 22 307 7.58e-35 129
MS.gene053809.t1 AT2G45400 34.722 216 131 5 5 211 41 255 1.71e-31 119
MS.gene053809.t1 AT2G23910 25.830 271 184 7 5 269 11 270 3.26e-30 115
MS.gene053809.t1 AT2G23910 25.830 271 184 7 5 269 11 270 4.07e-30 115
MS.gene053809.t1 AT2G23910 25.830 271 184 7 5 269 11 270 5.58e-30 116
MS.gene053809.t1 AT4G30470 26.119 268 186 6 5 269 11 269 2.26e-27 108
MS.gene053809.t1 AT5G14700 29.588 267 155 7 5 251 56 309 3.43e-27 109
MS.gene053809.t1 AT2G23910 26.724 232 153 7 44 269 16 236 4.86e-25 102
MS.gene053809.t1 AT5G14700 30.734 218 124 5 5 205 56 263 3.66e-23 97.4

Find 72 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGTATTATTATCTCCTTTC+TGG 0.077666 5.1:+44418450 None:intergenic
CGTGATTGCTGCCCACTTAT+TGG 0.174389 5.1:-44418690 MS.gene053809:CDS
AAATCCCAGTTGAGTGATTT+TGG 0.206011 5.1:+44418412 None:intergenic
TTGATTGATCCATGTATAAA+AGG 0.233674 5.1:-44419491 MS.gene053809:CDS
TGCAGGAGTTCAGCACATTC+TGG 0.245081 5.1:+44419465 None:intergenic
TTATAGCGTTTGCAGTATTC+TGG 0.245242 5.1:+44419335 None:intergenic
CACATCGTCGCGGTATCTTA+TGG 0.246307 5.1:+44419397 None:intergenic
GTACGGATAAGAAGGATATT+TGG 0.250292 5.1:+44418583 None:intergenic
AAATTAATTTGCAGATGATT+TGG 0.284567 5.1:-44420490 MS.gene053809:intron
GTTAAAGCCTTATTAGAAAA+AGG 0.298250 5.1:-44420635 MS.gene053809:CDS
CAGCAATCACGTCATCTATA+TGG 0.329414 5.1:+44418702 None:intergenic
TCCATATGATAACAACATTC+AGG 0.350735 5.1:-44420325 MS.gene053809:intron
GATATTTGGCCTGAAGCATT+TGG 0.356020 5.1:+44418597 None:intergenic
GTTAACCCCTCTTTCGTTGT+TGG 0.364774 5.1:-44418927 MS.gene053809:CDS
CACCACCAAAATCACTCAAC+TGG 0.366902 5.1:-44418417 MS.gene053809:CDS
TACAAGCCTGCCAGATGCTT+TGG 0.370462 5.1:+44418655 None:intergenic
TGCTGCCCACTTATTGGCTA+TGG 0.389523 5.1:-44418684 MS.gene053809:CDS
TCAAATCATCCAAATGCTTC+AGG 0.391082 5.1:-44418606 MS.gene053809:CDS
CTCCAATGAGATTCATTAAG+AGG 0.392826 5.1:+44419362 None:intergenic
CTCCTCTTAATGAATCTCAT+TGG 0.405823 5.1:-44419364 MS.gene053809:CDS
TGTGTTTGGGTAATCTCCTC+TGG 0.409767 5.1:+44418739 None:intergenic
ACAAATCCCACTGTTGTGTT+TGG 0.410589 5.1:+44418725 None:intergenic
ACCTGAATGTTGTTATCATA+TGG 0.449261 5.1:+44420324 None:intergenic
TTGATTTCGTACGGATAAGA+AGG 0.451640 5.1:+44418575 None:intergenic
GCAGGAGTTCAGCACATTCT+GGG 0.454583 5.1:+44419466 None:intergenic
GAGGCAGTGACTGGGGTTGA+TGG 0.459132 5.1:-44420378 MS.gene053809:CDS
GCAGATGATTTGGAGAAGGT+TGG 0.460715 5.1:-44420480 MS.gene053809:intron
ATGGAGGAGTCCAAAGCATC+TGG 0.477461 5.1:-44418665 MS.gene053809:CDS
CAAATCCCACTGTTGTGTTT+GGG 0.480222 5.1:+44418726 None:intergenic
TTTCACATTTGCTTTGATGC+AGG 0.485464 5.1:+44419448 None:intergenic
TAAACTTACTTGATTTCGTA+CGG 0.486707 5.1:+44418566 None:intergenic
AGCAATGTGCTAGCTGGCTG+TGG 0.489492 5.1:+44418891 None:intergenic
GCTGGCTGTGGTGCAAGGAG+TGG 0.505517 5.1:+44418903 None:intergenic
TGGGAGAAAGAGAGGCTTGG+AGG 0.506920 5.1:-44418983 MS.gene053809:CDS
TTTCTAATAAGGCTTTAACT+AGG 0.507635 5.1:+44420639 None:intergenic
TATAGGTGTAGCAGCCAGAA+AGG 0.509846 5.1:-44418464 MS.gene053809:intron
TATGCATATGCAAAGACATT+GGG 0.522633 5.1:-44419002 MS.gene053809:CDS
TGCTAGCTGGCTGTGGTGCA+AGG 0.531358 5.1:+44418898 None:intergenic
TGGAGGATTGCAGAGGAAAG+TGG 0.531373 5.1:-44418966 MS.gene053809:CDS
ATCAAAAGCTTTCAAGACAA+AGG 0.539091 5.1:-44418350 MS.gene053809:CDS
ATTTGCAGATGATTTGGAGA+AGG 0.544790 5.1:-44420484 MS.gene053809:intron
GGAAAGTGGGCTTGATCTAG+TGG 0.545333 5.1:-44418952 MS.gene053809:CDS
ACTCCTCCATAGCCAATAAG+TGG 0.545932 5.1:+44418678 None:intergenic
ATCATGAGCAATGTGCTAGC+TGG 0.548471 5.1:+44418885 None:intergenic
CATTGGGAGAAAGAGAGGCT+TGG 0.548941 5.1:-44418986 MS.gene053809:CDS
TTATCATATGGAACAATCAC+AGG 0.553452 5.1:+44420336 None:intergenic
CCTCCTGTCACGCAAAACTC+AGG 0.555398 5.1:+44420683 None:intergenic
TTGGATGATTTGAGACCAGT+GGG 0.557451 5.1:+44418616 None:intergenic
TTTGCGTGACAGGAGGCACT+GGG 0.559306 5.1:-44420676 MS.gene053809:CDS
GGAGGATTGCAGAGGAAAGT+GGG 0.562235 5.1:-44418965 MS.gene053809:CDS
TGCCCACTTATTGGCTATGG+AGG 0.564412 5.1:-44418681 MS.gene053809:CDS
GAGTGGACCAACAACGAAAG+AGG 0.567282 5.1:+44418920 None:intergenic
AAGCAGATCTGATGGTGGAA+GGG 0.569525 5.1:-44420410 MS.gene053809:CDS
AAAGCAGATCTGATGGTGGA+AGG 0.575224 5.1:-44420411 MS.gene053809:CDS
GTTCAAGCACAGTTGCCCAC+TGG 0.575383 5.1:-44418631 MS.gene053809:CDS
TTTGGATGATTTGAGACCAG+TGG 0.578890 5.1:+44418615 None:intergenic
AGGAGTCCAAAGCATCTGGC+AGG 0.581188 5.1:-44418661 MS.gene053809:CDS
AGTGGACCAACAACGAAAGA+GGG 0.581353 5.1:+44418921 None:intergenic
AGATAATAATACGCATAGCA+TGG 0.591714 5.1:-44418441 MS.gene053809:CDS
GTATGCATATGCAAAGACAT+TGG 0.592386 5.1:-44419003 MS.gene053809:CDS
AAAGACATTGGGAGAAAGAG+AGG 0.610985 5.1:-44418991 MS.gene053809:CDS
ACCACCAAAATCACTCAACT+GGG 0.612238 5.1:-44418416 MS.gene053809:CDS
GATTACCCAAACACAACAGT+GGG 0.612907 5.1:-44418731 MS.gene053809:CDS
ATGATACTAAGCATCATCAA+AGG 0.628815 5.1:-44418867 MS.gene053809:intron
CTCCTCCATAGCCAATAAGT+GGG 0.636852 5.1:+44418679 None:intergenic
TTTAAAAGCAGATCTGATGG+TGG 0.645545 5.1:-44420415 MS.gene053809:CDS
AAACTAGTTGCACATCGTCG+CGG 0.647555 5.1:+44419387 None:intergenic
AGAGGCTTGGAGGATTGCAG+AGG 0.653350 5.1:-44418973 MS.gene053809:CDS
AGAACTACAGTAAGAAACCC+AGG 0.661092 5.1:-44420602 MS.gene053809:intron
GTGGACCAACAACGAAAGAG+GGG 0.683072 5.1:+44418922 None:intergenic
AGATTACCCAAACACAACAG+TGG 0.691160 5.1:-44418732 MS.gene053809:CDS
CAAAGCAAATGTGAAACGTG+TGG 0.704173 5.1:-44419441 MS.gene053809:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTAATCTAAAAAATAAC+AGG - chr5.1:44420262-44420281 MS.gene053809:intron 10.0%
!! TCTATATAATATCAAAATTA+AGG + chr5.1:44420516-44420535 None:intergenic 10.0%
!!! ATAAAAATTAAGTATTGATA+CGG + chr5.1:44418856-44418875 None:intergenic 10.0%
!!! GATATTTATACAATTATTTT+GGG - chr5.1:44418789-44418808 MS.gene053809:intron 10.0%
!!! TGATATTTATACAATTATTT+TGG - chr5.1:44418788-44418807 MS.gene053809:intron 10.0%
!!! TTGAGTATTATTTTAAAATT+TGG + chr5.1:44419909-44419928 None:intergenic 10.0%
!! AAACATCATCAATATAGATA+TGG - chr5.1:44419476-44419495 MS.gene053809:CDS 20.0%
!! AATGTACCAATCAAATTAAT+AGG + chr5.1:44418475-44418494 None:intergenic 20.0%
!! ACTTAATGTCATAAGAAATA+TGG + chr5.1:44420240-44420259 None:intergenic 20.0%
!! ATCACTAAATATTAATACCT+GGG + chr5.1:44418444-44418463 None:intergenic 20.0%
!! TATCACTAAATATTAATACC+TGG + chr5.1:44418445-44418464 None:intergenic 20.0%
!! TTTGACAGAAAAATTAAAAG+TGG + chr5.1:44418920-44418939 None:intergenic 20.0%
!!! AAATTAATTTGCAGATGATT+TGG - chr5.1:44418536-44418555 MS.gene053809:intron 20.0%
!!! TATACTTTCTTTTCTACTAT+AGG - chr5.1:44420545-44420564 MS.gene053809:intron 20.0%
!!! TGATGTTTTTTTTTTTGAAG+AGG + chr5.1:44419464-44419483 None:intergenic 20.0%
! ACAAAAAAACACGACTATTA+CGG - chr5.1:44419279-44419298 MS.gene053809:intron 25.0%
! AGTCATATCAAATTCTTGTT+TGG - chr5.1:44419749-44419768 MS.gene053809:intron 25.0%
! CAAGAATTTGATATGACTTA+AGG + chr5.1:44419747-44419766 None:intergenic 25.0%
! CATAAATCACTTTACATAGA+AGG - chr5.1:44419058-44419077 MS.gene053809:intron 25.0%
! CGGATAAAATTTGCATATTA+AGG - chr5.1:44419302-44419321 MS.gene053809:intron 25.0%
! CTCTCTGTAACTAAATATTT+GGG + chr5.1:44419188-44419207 None:intergenic 25.0%
! TAAACTTACTTGATTTCGTA+CGG + chr5.1:44420463-44420482 None:intergenic 25.0%
! TCTCTCTGTAACTAAATATT+TGG + chr5.1:44419189-44419208 None:intergenic 25.0%
! TTGATTGATCCATGTATAAA+AGG - chr5.1:44419535-44419554 MS.gene053809:intron 25.0%
!! ACACATCCTATTAATTTGAT+TGG - chr5.1:44418466-44418485 MS.gene053809:intron 25.0%
!! TATGTGACCTTTTTCTAATA+AGG + chr5.1:44418401-44418420 None:intergenic 25.0%
!! TGCTATTTGAAATATGTTGT+AGG - chr5.1:44419507-44419526 MS.gene053809:intron 25.0%
!! TTAAGGCTTTATAATTGAGA+AGG + chr5.1:44419730-44419749 None:intergenic 25.0%
!! TTTCTAATAAGGCTTTAACT+AGG + chr5.1:44418390-44418409 None:intergenic 25.0%
!!! GTTAAAGCCTTATTAGAAAA+AGG - chr5.1:44418391-44418410 MS.gene053809:CDS 25.0%
!!! TTATTTTGCGAAAAAACAAG+TGG + chr5.1:44418515-44418534 None:intergenic 25.0%
AATCACTTTACATAGAAGGA+TGG - chr5.1:44419062-44419081 MS.gene053809:intron 30.0%
ACCTGAATGTTGTTATCATA+TGG + chr5.1:44418705-44418724 None:intergenic 30.0%
AGATAATAATACGCATAGCA+TGG - chr5.1:44420585-44420604 MS.gene053809:intron 30.0%
AGTAATGTCACATTCTTGTT+TGG - chr5.1:44419824-44419843 MS.gene053809:intron 30.0%
ATCAAAAGCTTTCAAGACAA+AGG - chr5.1:44420676-44420695 MS.gene053809:CDS 30.0%
ATGATACTAAGCATCATCAA+AGG - chr5.1:44420159-44420178 MS.gene053809:intron 30.0%
TAAAAAATAACAGGTTCCAG+AGG - chr5.1:44420271-44420290 MS.gene053809:intron 30.0%
TATGCATATGCAAAGACATT+GGG - chr5.1:44420024-44420043 MS.gene053809:intron 30.0%
TCCATATGATAACAACATTC+AGG - chr5.1:44418701-44418720 MS.gene053809:CDS 30.0%
TTAACTGAACAACAAACACA+AGG + chr5.1:44419017-44419036 None:intergenic 30.0%
TTATCATATGGAACAATCAC+AGG + chr5.1:44418693-44418712 None:intergenic 30.0%
!! GATTTTAAAAGCAGATCTGA+TGG - chr5.1:44418608-44418627 MS.gene053809:CDS 30.0%
!!! GTCTGTTACATTAGTCTTTT+TGG - chr5.1:44419939-44419958 MS.gene053809:intron 30.0%
!!! TGTTCAAGAGTTTTGAATTG+AGG + chr5.1:44420640-44420659 None:intergenic 30.0%
AAAAAACACGACTATTACGG+CGG - chr5.1:44419282-44419301 MS.gene053809:intron 35.0%
CTCCAATGAGATTCATTAAG+AGG + chr5.1:44419667-44419686 None:intergenic 35.0%
CTCCTCTTAATGAATCTCAT+TGG - chr5.1:44419662-44419681 MS.gene053809:intron 35.0%
GCGTATTATTATCTCCTTTC+TGG + chr5.1:44420579-44420598 None:intergenic 35.0%
GTACGGATAAGAAGGATATT+TGG + chr5.1:44420446-44420465 None:intergenic 35.0%
GTATGCATATGCAAAGACAT+TGG - chr5.1:44420023-44420042 MS.gene053809:intron 35.0%
TCAAATCATCCAAATGCTTC+AGG - chr5.1:44420420-44420439 MS.gene053809:CDS 35.0%
TCACAAAAAGTTCACGCATA+TGG + chr5.1:44419126-44419145 None:intergenic 35.0%
TTATAGCGTTTGCAGTATTC+TGG + chr5.1:44419694-44419713 None:intergenic 35.0%
TTGATTTCGTACGGATAAGA+AGG + chr5.1:44420454-44420473 None:intergenic 35.0%
TTTAAAAGCAGATCTGATGG+TGG - chr5.1:44418611-44418630 MS.gene053809:CDS 35.0%
TTTCACATTTGCTTTGATGC+AGG + chr5.1:44419581-44419600 None:intergenic 35.0%
! AAATCCCAGTTGAGTGATTT+TGG + chr5.1:44420617-44420636 None:intergenic 35.0%
! ATTTGCAGATGATTTGGAGA+AGG - chr5.1:44418542-44418561 MS.gene053809:intron 35.0%
! TTCTGGGTTCCTTTTATACA+TGG + chr5.1:44419547-44419566 None:intergenic 35.0%
! TTTTCGCATCAAGTAACCAT+AGG - chr5.1:44419151-44419170 MS.gene053809:intron 35.0%
!! AACCGCCATTTTAAACTTTG+CGG + chr5.1:44419388-44419407 None:intergenic 35.0%
!! TACGACATTTTTGAAAACGC+CGG + chr5.1:44419355-44419374 None:intergenic 35.0%
AAAATGGCGGTTTGTAGAGA+TGG - chr5.1:44419396-44419415 MS.gene053809:CDS 40.0%
AAAGACATTGGGAGAAAGAG+AGG - chr5.1:44420035-44420054 MS.gene053809:intron 40.0%
ACAAATCCCACTGTTGTGTT+TGG + chr5.1:44420304-44420323 None:intergenic 40.0%
ACCACCAAAATCACTCAACT+GGG - chr5.1:44420610-44420629 MS.gene053809:CDS 40.0%
ACTTGTGAGTTATGCAGCTT+TGG - chr5.1:44420001-44420020 MS.gene053809:intron 40.0%
AGAACTACAGTAAGAAACCC+AGG - chr5.1:44418424-44418443 MS.gene053809:CDS 40.0%
AGATTACCCAAACACAACAG+TGG - chr5.1:44420294-44420313 MS.gene053809:intron 40.0%
CAAAGCAAATGTGAAACGTG+TGG - chr5.1:44419585-44419604 MS.gene053809:intron 40.0%
CAAATCCCACTGTTGTGTTT+GGG + chr5.1:44420303-44420322 None:intergenic 40.0%
CAGCAATCACGTCATCTATA+TGG + chr5.1:44420327-44420346 None:intergenic 40.0%
GATTACCCAAACACAACAGT+GGG - chr5.1:44420295-44420314 MS.gene053809:intron 40.0%
TTGGGACACATTTCATCCTA+TGG + chr5.1:44419170-44419189 None:intergenic 40.0%
! GATATTTGGCCTGAAGCATT+TGG + chr5.1:44420432-44420451 None:intergenic 40.0%
! TGCCGCAAAGTTTAAAATGG+CGG - chr5.1:44419383-44419402 MS.gene053809:CDS 40.0%
! TTGGATGATTTGAGACCAGT+GGG + chr5.1:44420413-44420432 None:intergenic 40.0%
! TTTGGATGATTTGAGACCAG+TGG + chr5.1:44420414-44420433 None:intergenic 40.0%
!! CATTTTTGAAAACGCCGGAA+TGG + chr5.1:44419350-44419369 None:intergenic 40.0%
AAACTAGTTGCACATCGTCG+CGG + chr5.1:44419642-44419661 None:intergenic 45.0%
AACGCCGGAATGGTTTATAG+TGG + chr5.1:44419340-44419359 None:intergenic 45.0%
ACTCCTCCATAGCCAATAAG+TGG + chr5.1:44420351-44420370 None:intergenic 45.0%
AGTGGACCAACAACGAAAGA+GGG + chr5.1:44420108-44420127 None:intergenic 45.0%
ATCATGAGCAATGTGCTAGC+TGG + chr5.1:44420144-44420163 None:intergenic 45.0%
CACCACCAAAATCACTCAAC+TGG - chr5.1:44420609-44420628 MS.gene053809:CDS 45.0%
CTCCTCCATAGCCAATAAGT+GGG + chr5.1:44420350-44420369 None:intergenic 45.0%
GCCACCACTATAAACCATTC+CGG - chr5.1:44419333-44419352 MS.gene053809:intron 45.0%
GCCTGCCGCAAAGTTTAAAA+TGG - chr5.1:44419380-44419399 MS.gene053809:CDS 45.0%
GTTAACCCCTCTTTCGTTGT+TGG - chr5.1:44420099-44420118 MS.gene053809:intron 45.0%
TATAGGTGTAGCAGCCAGAA+AGG - chr5.1:44420562-44420581 MS.gene053809:intron 45.0%
TGTGTTTGGGTAATCTCCTC+TGG + chr5.1:44420290-44420309 None:intergenic 45.0%
! GCAGATGATTTGGAGAAGGT+TGG - chr5.1:44418546-44418565 MS.gene053809:intron 45.0%
! TCCCAGTTGAGTGATTTTGG+TGG + chr5.1:44420614-44420633 None:intergenic 45.0%
! TTTTGATGAGGCAGTGACTG+GGG - chr5.1:44418641-44418660 MS.gene053809:CDS 45.0%
!! AAAGCAGATCTGATGGTGGA+AGG - chr5.1:44418615-44418634 MS.gene053809:CDS 45.0%
!! AAGCAGATCTGATGGTGGAA+GGG - chr5.1:44418616-44418635 MS.gene053809:CDS 45.0%
!! AGTTTTGATGAGGCAGTGAC+TGG - chr5.1:44418639-44418658 MS.gene053809:CDS 45.0%
!! GCCATTTTAAACTTTGCGGC+AGG + chr5.1:44419384-44419403 None:intergenic 45.0%
!! GTTTTGATGAGGCAGTGACT+GGG - chr5.1:44418640-44418659 MS.gene053809:CDS 45.0%
CACATCGTCGCGGTATCTTA+TGG + chr5.1:44419632-44419651 None:intergenic 50.0%
CATTGGGAGAAAGAGAGGCT+TGG - chr5.1:44420040-44420059 MS.gene053809:intron 50.0%
CGTGATTGCTGCCCACTTAT+TGG - chr5.1:44420336-44420355 MS.gene053809:CDS 50.0%
GAGTGGACCAACAACGAAAG+AGG + chr5.1:44420109-44420128 None:intergenic 50.0%
GCAGGAGTTCAGCACATTCT+GGG + chr5.1:44419563-44419582 None:intergenic 50.0%
GCCGGAATGGTTTATAGTGG+TGG + chr5.1:44419337-44419356 None:intergenic 50.0%
GGAGGATTGCAGAGGAAAGT+GGG - chr5.1:44420061-44420080 MS.gene053809:intron 50.0%
GTGGACCAACAACGAAAGAG+GGG + chr5.1:44420107-44420126 None:intergenic 50.0%
TACAAGCCTGCCAGATGCTT+TGG + chr5.1:44420374-44420393 None:intergenic 50.0%
TGCAGGAGTTCAGCACATTC+TGG + chr5.1:44419564-44419583 None:intergenic 50.0%
TGGAGGATTGCAGAGGAAAG+TGG - chr5.1:44420060-44420079 MS.gene053809:intron 50.0%
! ATGCCTGAGTTTTGCGTGAC+AGG - chr5.1:44418340-44418359 MS.gene053809:CDS 50.0%
! ATGGAGGAGTCCAAAGCATC+TGG - chr5.1:44420361-44420380 MS.gene053809:CDS 50.0%
! GGAAAGTGGGCTTGATCTAG+TGG - chr5.1:44420074-44420093 MS.gene053809:intron 50.0%
! TGCCCACTTATTGGCTATGG+AGG - chr5.1:44420345-44420364 MS.gene053809:CDS 50.0%
! TGCTGCCCACTTATTGGCTA+TGG - chr5.1:44420342-44420361 MS.gene053809:CDS 50.0%
!! GGTGGAAGGGAGTTTTGATG+AGG - chr5.1:44418629-44418648 MS.gene053809:CDS 50.0%
AGAGGCTTGGAGGATTGCAG+AGG - chr5.1:44420053-44420072 MS.gene053809:intron 55.0%
AGCAATGTGCTAGCTGGCTG+TGG + chr5.1:44420138-44420157 None:intergenic 55.0%
CCTCCTGTCACGCAAAACTC+AGG + chr5.1:44418346-44418365 None:intergenic 55.0%
GTTCAAGCACAGTTGCCCAC+TGG - chr5.1:44420395-44420414 MS.gene053809:CDS 55.0%
TGGGAGAAAGAGAGGCTTGG+AGG - chr5.1:44420043-44420062 MS.gene053809:intron 55.0%
! AGGAGTCCAAAGCATCTGGC+AGG - chr5.1:44420365-44420384 MS.gene053809:CDS 55.0%
! CCTGAGTTTTGCGTGACAGG+AGG - chr5.1:44418343-44418362 MS.gene053809:CDS 55.0%
!! TTTGCGTGACAGGAGGCACT+GGG - chr5.1:44418350-44418369 MS.gene053809:CDS 55.0%
!! TTTTGCGTGACAGGAGGCAC+TGG - chr5.1:44418349-44418368 MS.gene053809:CDS 55.0%
GAGGCAGTGACTGGGGTTGA+TGG - chr5.1:44418648-44418667 MS.gene053809:CDS 60.0%
TGCTAGCTGGCTGTGGTGCA+AGG + chr5.1:44420131-44420150 None:intergenic 60.0%
GCTGGCTGTGGTGCAAGGAG+TGG + chr5.1:44420126-44420145 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 44418340 44420708 44418340 ID=MS.gene053809
chr5.1 mRNA 44418340 44420708 44418340 ID=MS.gene053809.t1;Parent=MS.gene053809
chr5.1 exon 44420603 44420708 44420603 ID=MS.gene053809.t1.exon1;Parent=MS.gene053809.t1
chr5.1 CDS 44420603 44420708 44420603 ID=cds.MS.gene053809.t1;Parent=MS.gene053809.t1
chr5.1 exon 44420326 44420498 44420326 ID=MS.gene053809.t1.exon2;Parent=MS.gene053809.t1
chr5.1 CDS 44420326 44420498 44420326 ID=cds.MS.gene053809.t1;Parent=MS.gene053809.t1
chr5.1 exon 44419334 44419519 44419334 ID=MS.gene053809.t1.exon3;Parent=MS.gene053809.t1
chr5.1 CDS 44419334 44419519 44419334 ID=cds.MS.gene053809.t1;Parent=MS.gene053809.t1
chr5.1 exon 44418868 44419030 44418868 ID=MS.gene053809.t1.exon4;Parent=MS.gene053809.t1
chr5.1 CDS 44418868 44419030 44418868 ID=cds.MS.gene053809.t1;Parent=MS.gene053809.t1
chr5.1 exon 44418575 44418764 44418575 ID=MS.gene053809.t1.exon5;Parent=MS.gene053809.t1
chr5.1 CDS 44418575 44418764 44418575 ID=cds.MS.gene053809.t1;Parent=MS.gene053809.t1
chr5.1 exon 44418340 44418481 44418340 ID=MS.gene053809.t1.exon6;Parent=MS.gene053809.t1
chr5.1 CDS 44418340 44418481 44418340 ID=cds.MS.gene053809.t1;Parent=MS.gene053809.t1
Gene Sequence

>MS.gene053809

ATGCCTGAGTTTTGCGTGACAGGAGGCACTGGGTTCATAGCAGCTTACCTAGTTAAAGCCTTATTAGAAAAAGGTCACATAGTAAGAACTACAGTAAGAAACCCAGGTATTAATATTTAGTGATATACACATCCTATTAATTTGATTGGTACATTAATTTTTCATTTTTAATCCACTTGTTTTTTCGCAAAATAAAAAATTAATTTGCAGATGATTTGGAGAAGGTTGGTTATCTAACTCAACTAAGTAGAGACAAAGAAAGATTAAAGATTTTAAAAGCAGATCTGATGGTGGAAGGGAGTTTTGATGAGGCAGTGACTGGGGTTGATGGTGTGTTTCATACAGCATCTCCTGTGATTGTTCCATATGATAACAACATTCAGGTACACTTTTCTATTTCTTATAAAAGTGTGTAATTAATTTTGAATTGTAGTTTTAATTTGAGAAATGATATTTATACAATTATTTTGGGATAACTTTTGTGACAATTTTCTGTCTCTGATCGTTTGATTTCCGTATCAATACTTAATTTTTATGTAAATTTATTGTTGTCGCACAAATTATTGTTCAAATAACACCACTTTTAATTTTTCTGTCAAAATTAAATAATTATGAATTGTAGTTTTTATTTTGCTTTCGAAATTGTTGTGTTTGATGTCTAGAGTTGATTTTAACCTTGTGTTTGTTGTTCAGTTAACTTTTCATAAATACAGCGAAACATAAATCACTTTACATAGAAGGATGGTTGCAAATAAAAATGATGAGCTGCTACTGTTACAGTAACCATATGCGTGAACTTTTTGTGAATTATTTTTCGCATCAAGTAACCATAGGATGAAATGTGTCCCAAATATTTAGTTACAGAGAGAAATTGATGTGAGACTACTTTGTAAATTCAGAAAGTAATTCAATAAAAAATTGTCTTTGTTAATAGACACTACAAAAAAACACGACTATTACGGCGGATAAAATTTGCATATTAAGGCAGTTTTAGCCACCACTATAAACCATTCCGGCGTTTTCAAAAATGTCGTAATTTTGCCTGCCGCAAAGTTTAAAATGGCGGTTTGTAGAGATGGTATATATTTTCATCTTTTCAATAACATAACATCATCAATATACCTCTTCAAAAAAAAAAACATCATCAATATAGATATGGTAATTTGTTGCTATTTGAAATATGTTGTAGGCTACTTTGATTGATCCATGTATAAAAGGAACCCAGAATGTGCTGAACTCCTGCATCAAAGCAAATGTGAAACGTGTGGTGTTAACATCTTCTTGCTCTTCCATAAGATACCGCGACGATGTGCAACTAGTTTCTCCTCTTAATGAATCTCATTGGAGTGATCCAGAATACTGCAAACGCTATAATGTAACAACACTTCCTTCTCAATTATAAAGCCTTAAGTCATATCAAATTCTTGTTTGGATCATCACTTTGAGTAATATAGTTTACAAGAACTACATATATCATCACTTTAAGTAATGTCACATTCTTGTTTGGATCATAACTTTGAATTTTATGTTTTCTTTTTCAATATTTTTGAATCATTTTGAAACTAACCAAATTTTAAAATAATACTCAAACTAAAAATAGTCTGTTACATTAGTCTTTTTGGATCAATCAATTTAAATTAGTGTAATTTAAATTCAATGAAACTTGTGAGTTATGCAGCTTTGGTATGCATATGCAAAGACATTGGGAGAAAGAGAGGCTTGGAGGATTGCAGAGGAAAGTGGGCTTGATCTAGTGGTTGTTAACCCCTCTTTCGTTGTTGGTCCACTCCTTGCACCACAGCCAGCTAGCACATTGCTCATGATACTAAGCATCATCAAAGGTTATATAATTCTCTCTCAAATGTGTTTTGATGTTAAGAATTCTTTGTATTCTATACCATATTTCTTATGACATTAAGTGTTATTTAATCTAAAAAATAACAGGTTCCAGAGGAGATTACCCAAACACAACAGTGGGATTTGTCCATATAGATGACGTGATTGCTGCCCACTTATTGGCTATGGAGGAGTCCAAAGCATCTGGCAGGCTTGTATGTTCAAGCACAGTTGCCCACTGGTCTCAAATCATCCAAATGCTTCAGGCCAAATATCCTTCTTATCCGTACGAAATCAAGTAAGTTTAAGAATTGCTGTATATCATATACTATATCAACCTTAATTTTGATATTATATAGAAAAAGTATTTATACTTTCTTTTCTACTATAGGTGTAGCAGCCAGAAAGGAGATAATAATACGCATAGCATGGACACCACCAAAATCACTCAACTGGGATTTCCTCAATTCAAAACTCTTGAACAAATGTTTGATGACTGCATCAAAAGCTTTCAAGACAAAGGCTTCCTATGA
Protein sequence

>MS.gene053809.t1

MPEFCVTGGTGFIAAYLVKALLEKGHIVRTTVRNPDDLEKVGYLTQLSRDKERLKILKADLMVEGSFDEAVTGVDGVFHTASPVIVPYDNNIQATLIDPCIKGTQNVLNSCIKANVKRVVLTSSCSSIRYRDDVQLVSPLNESHWSDPEYCKRYNLWYAYAKTLGEREAWRIAEESGLDLVVVNPSFVVGPLLAPQPASTLLMILSIIKGSRGDYPNTTVGFVHIDDVIAAHLLAMEESKASGRLVCSSTVAHWSQIIQMLQAKYPSYPYEIKCSSQKGDNNTHSMDTTKITQLGFPQFKTLEQMFDDCIKSFQDKGFL