AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65685

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65685.t1 MTR_5g056600 96.865 319 10 0 1 319 1 319 0.0 653
MS.gene65685.t1 MTR_3g005210 41.640 317 180 5 5 319 7 320 1.59e-86 263
MS.gene65685.t1 MTR_2g101330 42.271 317 178 4 5 319 6 319 8.16e-84 256
MS.gene65685.t1 MTR_8g062440 42.006 319 175 6 5 319 9 321 1.51e-83 255
MS.gene65685.t1 MTR_3g005170 41.325 317 179 6 5 318 11 323 1.81e-83 255
MS.gene65685.t1 MTR_8g062110 43.038 316 170 6 5 316 10 319 1.17e-82 253
MS.gene65685.t1 MTR_8g062110 43.038 316 170 6 5 316 19 328 2.24e-82 253
MS.gene65685.t1 MTR_8g062440 41.875 320 175 7 5 319 9 322 1.52e-81 250
MS.gene65685.t1 MTR_3g005170 45.113 266 140 5 5 267 11 273 5.17e-79 242
MS.gene65685.t1 MTR_8g062440 43.390 295 158 5 5 295 9 298 2.66e-77 239
MS.gene65685.t1 MTR_8g062110 46.442 267 134 5 5 267 10 271 4.33e-77 237
MS.gene65685.t1 MTR_8g062110 46.442 267 134 5 5 267 10 271 5.67e-77 237
MS.gene65685.t1 MTR_2g104960 43.396 318 170 7 4 319 20 329 9.77e-77 238
MS.gene65685.t1 MTR_4g006940 43.038 316 172 5 5 319 18 326 8.83e-76 236
MS.gene65685.t1 MTR_1g022440 38.750 320 188 6 5 319 9 325 6.90e-75 233
MS.gene65685.t1 MTR_1g022445 38.750 320 188 6 5 319 9 325 2.89e-73 229
MS.gene65685.t1 MTR_4g077130 39.873 316 185 5 5 318 10 322 1.98e-72 227
MS.gene65685.t1 MTR_4g077100 40.063 317 183 6 5 318 12 324 5.69e-72 226
MS.gene65685.t1 MTR_5g072620 38.750 320 185 5 5 319 6 319 2.66e-71 224
MS.gene65685.t1 MTR_4g006940 45.353 269 141 3 5 273 18 280 4.19e-68 214
MS.gene65685.t1 MTR_4g006940 45.353 269 141 3 5 273 18 280 4.19e-68 214
MS.gene65685.t1 MTR_4g009040 36.449 321 195 5 3 319 12 327 1.22e-67 214
MS.gene65685.t1 MTR_3g005170 36.076 316 166 5 5 318 11 292 2.07e-67 213
MS.gene65685.t1 MTR_4g009040 36.250 320 193 5 3 319 12 323 1.27e-66 212
MS.gene65685.t1 MTR_7g074880 35.714 322 196 7 5 319 9 326 5.51e-66 210
MS.gene65685.t1 MTR_4g077100 42.105 266 148 5 5 267 12 274 7.93e-66 208
MS.gene65685.t1 MTR_3g031650 33.544 316 207 3 5 319 6 319 2.66e-63 203
MS.gene65685.t1 MTR_7g074820 37.231 325 190 8 5 319 9 329 1.44e-61 199
MS.gene65685.t1 MTR_7g074730 36.420 324 193 8 5 319 9 328 3.65e-61 198
MS.gene65685.t1 MTR_3g031650 36.364 264 168 0 5 268 6 269 8.03e-61 195
MS.gene65685.t1 MTR_4g081440 37.500 336 187 8 3 319 23 354 1.32e-59 195
MS.gene65685.t1 MTR_4g081440 37.202 336 187 8 3 319 19 349 2.93e-59 194
MS.gene65685.t1 MTR_4g009690 35.000 320 197 5 3 319 12 323 1.51e-58 191
MS.gene65685.t1 MTR_4g009690 35.312 320 195 6 3 319 12 322 2.07e-58 191
MS.gene65685.t1 MTR_7g074850 34.154 325 200 8 5 319 9 329 2.96e-55 182
MS.gene65685.t1 MTR_7g074710 33.639 327 196 7 5 319 9 326 4.66e-54 179
MS.gene65685.t1 MTR_4g092080 31.707 328 203 9 5 317 15 336 2.62e-52 175
MS.gene65685.t1 MTR_2g028620 31.464 321 209 5 5 319 11 326 3.87e-47 162
MS.gene65685.t1 MTR_7g074870 32.759 290 185 6 36 319 3 288 6.22e-47 160
MS.gene65685.t1 MTR_8g062110 37.719 228 133 5 93 316 11 233 2.47e-46 157
MS.gene65685.t1 MTR_2g028620 34.317 271 168 4 5 269 11 277 9.15e-46 157
MS.gene65685.t1 MTR_4g023730 31.173 324 211 7 5 319 9 329 2.49e-44 154
MS.gene65685.t1 MTR_8g062110 41.341 179 97 4 93 267 11 185 1.38e-40 140
MS.gene65685.t1 MTR_2g013250 28.487 337 210 8 5 317 8 337 5.11e-35 130
MS.gene65685.t1 MTR_2g013230 29.032 341 207 10 5 319 9 340 3.55e-33 125
MS.gene65685.t1 MTR_2g013260 27.536 345 203 9 5 317 9 338 3.50e-30 117
MS.gene65685.t1 MTR_6g406250 31.183 279 175 9 5 274 56 326 5.10e-30 117
MS.gene65685.t1 MTR_5g029990 26.800 250 157 7 5 247 13 243 4.02e-29 113
MS.gene65685.t1 MTR_0164s0070 44.531 128 70 1 3 129 4 131 1.84e-28 109
MS.gene65685.t1 MTR_7g117190 27.811 338 216 12 5 319 8 340 3.39e-28 112
MS.gene65685.t1 MTR_2g013260 27.907 301 172 8 5 273 9 296 8.91e-27 107
MS.gene65685.t1 MTR_0685s0030 34.969 163 104 2 156 318 1 161 1.39e-26 103
MS.gene65685.t1 MTR_6g406250 32.701 211 132 6 5 208 56 263 3.69e-26 104
MS.gene65685.t1 MTR_7g117190 28.421 285 182 10 5 268 8 291 3.24e-25 103
MS.gene65685.t1 MTR_0685s0030 37.500 112 69 1 156 267 1 111 1.96e-20 85.5
MS.gene65685.t1 MTR_3g011130 39.655 116 67 3 96 208 30 145 6.26e-20 85.1
MS.gene65685.t1 MTR_2g013230 25.403 248 151 9 98 319 11 250 2.51e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65685.t1 AT1G68540 73.832 321 82 1 1 319 1 321 0.0 511
MS.gene65685.t1 AT1G25460 64.486 321 110 3 1 319 1 319 2.03e-154 435
MS.gene65685.t1 AT1G68540 73.832 214 54 1 108 319 1 214 1.58e-121 348
MS.gene65685.t1 AT4G35420 47.634 317 160 5 5 319 9 321 8.91e-99 294
MS.gene65685.t1 AT1G51410 42.767 318 175 6 5 319 10 323 3.67e-87 265
MS.gene65685.t1 AT5G19440 42.089 316 178 5 5 318 11 323 1.27e-83 256
MS.gene65685.t1 AT1G09510 41.083 314 180 5 5 316 9 319 7.22e-76 236
MS.gene65685.t1 AT5G58490 37.618 319 190 4 5 319 10 323 9.65e-71 223
MS.gene65685.t1 AT1G15950 40.506 316 180 5 5 319 14 322 6.38e-70 221
MS.gene65685.t1 AT1G15950 40.506 316 180 5 5 319 14 322 6.38e-70 221
MS.gene65685.t1 AT1G09480 37.539 317 193 5 5 319 9 322 2.44e-68 216
MS.gene65685.t1 AT1G09480 37.539 317 193 5 5 319 56 369 7.57e-68 216
MS.gene65685.t1 AT1G09490 37.304 319 191 6 5 319 9 322 7.75e-68 215
MS.gene65685.t1 AT1G80820 39.557 316 183 4 5 319 9 317 1.56e-67 214
MS.gene65685.t1 AT1G09500 36.025 322 188 6 5 316 9 322 2.22e-66 211
MS.gene65685.t1 AT5G42800 37.578 322 189 8 5 319 9 325 6.47e-66 212
MS.gene65685.t1 AT1G15950 39.803 304 176 4 5 307 14 311 1.89e-65 209
MS.gene65685.t1 AT1G66800 37.461 323 181 8 5 319 9 318 5.17e-65 207
MS.gene65685.t1 AT1G09500 39.474 266 154 4 5 266 9 271 6.48e-64 203
MS.gene65685.t1 AT1G15950 42.007 269 150 3 5 273 14 276 8.03e-62 198
MS.gene65685.t1 AT2G02400 32.177 317 208 5 5 319 7 318 1.35e-61 199
MS.gene65685.t1 AT1G15950 42.264 265 147 3 5 269 14 272 2.01e-61 197
MS.gene65685.t1 AT1G15950 42.264 265 147 3 5 269 14 272 2.74e-61 197
MS.gene65685.t1 AT1G80820 40.149 269 155 2 5 273 9 271 3.31e-58 189
MS.gene65685.t1 AT2G33590 36.013 311 193 4 5 314 10 315 2.87e-56 185
MS.gene65685.t1 AT1G09500 34.471 293 174 6 34 316 4 288 5.94e-54 178
MS.gene65685.t1 AT1G09490 35.417 288 177 6 36 319 9 291 6.00e-54 178
MS.gene65685.t1 AT4G27250 33.735 332 178 9 4 310 13 327 1.42e-52 176
MS.gene65685.t1 AT4G27250 33.824 340 177 10 4 310 13 337 1.45e-52 176
MS.gene65685.t1 AT1G61720 33.934 333 193 8 5 319 14 337 1.38e-50 171
MS.gene65685.t1 AT2G33600 34.505 313 199 4 3 314 8 315 9.00e-50 168
MS.gene65685.t1 AT2G45400 33.436 326 204 8 5 319 41 364 4.66e-49 167
MS.gene65685.t1 AT1G76470 34.906 318 201 4 5 319 8 322 2.93e-47 162
MS.gene65685.t1 AT2G45400 35.036 274 167 6 5 268 41 313 2.87e-45 157
MS.gene65685.t1 AT1G15950 36.000 225 142 2 96 319 1 224 4.00e-41 144
MS.gene65685.t1 AT2G45400 31.597 288 186 7 41 319 22 307 1.42e-36 133
MS.gene65685.t1 AT2G45400 35.185 216 130 5 5 211 41 255 7.47e-32 119
MS.gene65685.t1 AT2G23910 26.199 271 183 7 5 269 11 270 3.53e-31 118
MS.gene65685.t1 AT2G23910 26.199 271 183 7 5 269 11 270 3.63e-31 118
MS.gene65685.t1 AT2G23910 26.199 271 183 7 5 269 11 270 4.90e-31 119
MS.gene65685.t1 AT5G14700 30.385 260 162 6 5 251 56 309 3.74e-28 112
MS.gene65685.t1 AT4G30470 26.866 268 184 6 5 269 11 269 4.03e-28 111
MS.gene65685.t1 AT2G23910 27.155 232 152 7 44 269 16 236 6.06e-26 104
MS.gene65685.t1 AT5G14700 31.754 211 131 4 5 205 56 263 6.74e-24 99.4
MS.gene65685.t1 AT1G47290 28.623 276 159 10 6 265 14 267 1.90e-16 79.7
MS.gene65685.t1 AT1G47290 28.623 276 159 10 6 265 14 267 8.19e-16 78.2

Find 75 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGTATTATTATCTCCTTTC+TGG 0.077666 5.2:+48520429 None:intergenic
CGTGATTGCTGCCCACTTAT+TGG 0.174389 5.2:-48520669 MS.gene65685:CDS
AAATCCCAGTTGAGTGATTT+TGG 0.206011 5.2:+48520391 None:intergenic
TTGATTGATCCATGTATAAA+AGG 0.233674 5.2:-48521470 MS.gene65685:CDS
TAAATAATTTGCAGATGATT+TGG 0.242771 5.2:-48522469 MS.gene65685:intron
TGCAGGAGTTCAGCACATTC+TGG 0.245081 5.2:+48521444 None:intergenic
TTATAGCGTTTGCAGTATTC+TGG 0.245242 5.2:+48521314 None:intergenic
CACATCGTCGCGGTATCTTA+TGG 0.246307 5.2:+48521376 None:intergenic
GTACGGATAAGAAGGATATT+TGG 0.250292 5.2:+48520562 None:intergenic
GTTAAAGCCTTATTAGAAAA+AGG 0.298250 5.2:-48522610 MS.gene65685:CDS
TACAAGCCTGCCAGATGCTT+TGG 0.319202 5.2:+48520634 None:intergenic
CAGCAATCACGTCATCTATA+TGG 0.329414 5.2:+48520681 None:intergenic
TCCATATGATAACAACATTC+AGG 0.350735 5.2:-48522304 MS.gene65685:intron
GATATTTGGCCTGAAGCATT+TGG 0.356020 5.2:+48520576 None:intergenic
GTTAACCCCTCTTTCGTTGT+TGG 0.364774 5.2:-48520906 MS.gene65685:CDS
CACCACCAAAATCACTCAAC+TGG 0.366902 5.2:-48520396 MS.gene65685:CDS
TGCTGCCCACTTATTGGCTA+TGG 0.389523 5.2:-48520663 MS.gene65685:CDS
TCAAATCATCCAAATGCTTC+AGG 0.391082 5.2:-48520585 MS.gene65685:CDS
CTCCAATGAGATTCATTAAG+AGG 0.392826 5.2:+48521341 None:intergenic
CTCCTCTTAATGAATCTCAT+TGG 0.405823 5.2:-48521343 MS.gene65685:CDS
TGTGTTTGGGTACTCTCCTC+TGG 0.407140 5.2:+48520718 None:intergenic
ACAAATCCCACTGTTGTGTT+TGG 0.410589 5.2:+48520704 None:intergenic
ACAAGCCTGCCAGATGCTTT+GGG 0.435678 5.2:+48520635 None:intergenic
ACCTGAATGTTGTTATCATA+TGG 0.449261 5.2:+48522303 None:intergenic
TTGATTTCGTACGGATAAGA+AGG 0.451640 5.2:+48520554 None:intergenic
CAAATCCCACTGTTGTGTTT+GGG 0.454369 5.2:+48520705 None:intergenic
GCAGGAGTTCAGCACATTCT+GGG 0.454583 5.2:+48521445 None:intergenic
GCAGATGATTTGGAGAAGGT+TGG 0.460715 5.2:-48522459 MS.gene65685:intron
GAGGCAGTGACTGGTGTTGA+TGG 0.476111 5.2:-48522357 MS.gene65685:CDS
ATGGAGGATCCCAAAGCATC+TGG 0.477050 5.2:-48520644 MS.gene65685:CDS
TAAACTTACTTGATTTCGTA+CGG 0.486707 5.2:+48520545 None:intergenic
AGCAATGTGCTAGCTGGCTG+TGG 0.489492 5.2:+48520870 None:intergenic
GCTGGCTGTGGTGCAAGGAG+TGG 0.505517 5.2:+48520882 None:intergenic
TGGGAGAAAGAGAGGCTTGG+AGG 0.506920 5.2:-48520962 MS.gene65685:CDS
TTTCTAATAAGGCTTTAACT+AGG 0.507635 5.2:+48522614 None:intergenic
TATAGGTGTAGCAGCCAGAA+AGG 0.509846 5.2:-48520443 MS.gene65685:intron
TATGCATATGCAAAGACATT+GGG 0.522633 5.2:-48520981 MS.gene65685:CDS
TTTCACGTTTGCTTTGATGC+AGG 0.527457 5.2:+48521427 None:intergenic
TGCCCACTTATTGGCTATGG+AGG 0.528146 5.2:-48520660 MS.gene65685:CDS
TGCTAGCTGGCTGTGGTGCA+AGG 0.531358 5.2:+48520877 None:intergenic
TGGAGGATTGCAGAGGAAAG+TGG 0.531373 5.2:-48520945 MS.gene65685:CDS
ATCAAAAGCTTTCAAGACAA+AGG 0.539091 5.2:-48520329 MS.gene65685:CDS
ATTTGCAGATGATTTGGAGA+AGG 0.544790 5.2:-48522463 MS.gene65685:intron
GGAAAGTGGGCTTGATCTAG+TGG 0.545333 5.2:-48520931 MS.gene65685:CDS
ATCATGAGCAATGTGCTAGC+TGG 0.548471 5.2:+48520864 None:intergenic
CATTGGGAGAAAGAGAGGCT+TGG 0.548941 5.2:-48520965 MS.gene65685:CDS
TTATCATATGGAACAATCAC+AGG 0.553452 5.2:+48522315 None:intergenic
CCTCCTGTCACGCAAAACTC+AGG 0.555398 5.2:+48522658 None:intergenic
TTGGATGATTTGAGACCAGT+GGG 0.557451 5.2:+48520595 None:intergenic
GATCCTCCATAGCCAATAAG+TGG 0.558490 5.2:+48520657 None:intergenic
TTTGCGTGACAGGAGGCACT+GGG 0.559306 5.2:-48522651 MS.gene65685:CDS
GGAGGATTGCAGAGGAAAGT+GGG 0.562235 5.2:-48520944 MS.gene65685:CDS
GAGTGGACCAACAACGAAAG+AGG 0.567282 5.2:+48520899 None:intergenic
AAGCAGATCTGATGGTGGAA+GGG 0.569525 5.2:-48522389 MS.gene65685:CDS
AAAGCAGATCTGATGGTGGA+AGG 0.575224 5.2:-48522390 MS.gene65685:CDS
TTTGGATGATTTGAGACCAG+TGG 0.578890 5.2:+48520594 None:intergenic
AGGATCCCAAAGCATCTGGC+AGG 0.580778 5.2:-48520640 MS.gene65685:CDS
AGTGGACCAACAACGAAAGA+GGG 0.581353 5.2:+48520900 None:intergenic
GTTCAACCACAGTTGCCCAC+TGG 0.588605 5.2:-48520610 MS.gene65685:CDS
AGATAATAATACGCATAGCA+TGG 0.591714 5.2:-48520420 MS.gene65685:CDS
GTATGCATATGCAAAGACAT+TGG 0.592386 5.2:-48520982 MS.gene65685:CDS
GAGTACCCAAACACAACAGT+GGG 0.606732 5.2:-48520710 MS.gene65685:CDS
AAAGACATTGGGAGAAAGAG+AGG 0.610985 5.2:-48520970 MS.gene65685:CDS
ACCACCAAAATCACTCAACT+GGG 0.612238 5.2:-48520395 MS.gene65685:CDS
TTGAGACCAGTGGGCAACTG+TGG 0.613180 5.2:+48520604 None:intergenic
ATGATACTAAGCATCATCAA+AGG 0.628815 5.2:-48520846 MS.gene65685:intron
ATCCTCCATAGCCAATAAGT+GGG 0.641325 5.2:+48520658 None:intergenic
TTTAAAAGCAGATCTGATGG+TGG 0.645545 5.2:-48522394 MS.gene65685:CDS
CAAAGCAAACGTGAAACGTG+TGG 0.647352 5.2:-48521420 MS.gene65685:CDS
AAACTTGTTGCACATCGTCG+CGG 0.647555 5.2:+48521366 None:intergenic
GGTTATCTAACTGAACTAAG+TGG 0.652651 5.2:-48522438 MS.gene65685:CDS
AGAGGCTTGGAGGATTGCAG+AGG 0.653350 5.2:-48520952 MS.gene65685:CDS
AGGACTACAGTAAGAAACCC+AGG 0.654613 5.2:-48522577 MS.gene65685:intron
GTGGACCAACAACGAAAGAG+GGG 0.683072 5.2:+48520901 None:intergenic
AGAGTACCCAAACACAACAG+TGG 0.693456 5.2:-48520711 MS.gene65685:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTAATCTAAAAAATAAC+AGG - chr5.2:48522237-48522256 MS.gene65685:intron 10.0%
!! TCTATATAATATCAAAATTA+AGG + chr5.2:48522491-48522510 None:intergenic 10.0%
!!! ATAAAAATTAAGTATTGATA+CGG + chr5.2:48520830-48520849 None:intergenic 10.0%
!!! GATATTTATACAATTATTTT+GGG - chr5.2:48520764-48520783 MS.gene65685:intron 10.0%
!!! TGATATTTATACAATTATTT+TGG - chr5.2:48520763-48520782 MS.gene65685:intron 10.0%
!!! TTGAGTATTATTTTAAAATT+TGG + chr5.2:48521884-48521903 None:intergenic 10.0%
!! AAACATCATCAATATAGATA+TGG - chr5.2:48521451-48521470 MS.gene65685:CDS 20.0%
!! AATGTACCAATCAAATTAAA+AGG + chr5.2:48520454-48520473 None:intergenic 20.0%
!! ACTTAATGTCATAAGAAATA+TGG + chr5.2:48522215-48522234 None:intergenic 20.0%
!! ATCACTAAATATTAATACCT+GGG + chr5.2:48520423-48520442 None:intergenic 20.0%
!! TATCACTAAATATTAATACC+TGG + chr5.2:48520424-48520443 None:intergenic 20.0%
!! TTTGACAGAAAAATTAAAAG+TGG + chr5.2:48520896-48520915 None:intergenic 20.0%
!!! ATGATGTTTTTTTTTTGAAG+AGG + chr5.2:48521440-48521459 None:intergenic 20.0%
!!! TAAATAATTTGCAGATGATT+TGG - chr5.2:48520511-48520530 MS.gene65685:intron 20.0%
!!! TATACTTTCTTTTCTACTAT+AGG - chr5.2:48522520-48522539 MS.gene65685:intron 20.0%
! ACAAAAAAACACGACTATTA+CGG - chr5.2:48521255-48521274 MS.gene65685:intron 25.0%
! AGTCATATCAAATTCTTGTT+TGG - chr5.2:48521724-48521743 MS.gene65685:intron 25.0%
! CAAGAATTTGATATGACTTA+AGG + chr5.2:48521722-48521741 None:intergenic 25.0%
! CATAAATCACTTTACATAGA+AGG - chr5.2:48521034-48521053 MS.gene65685:intron 25.0%
! CGGATAAAATTTGCATATTA+AGG - chr5.2:48521278-48521297 MS.gene65685:intron 25.0%
! CTCTCTGTAACTAAATATTT+GGG + chr5.2:48521164-48521183 None:intergenic 25.0%
! TAAACTTACTTGATTTCGTA+CGG + chr5.2:48522438-48522457 None:intergenic 25.0%
! TCTCTCTGTAACTAAATATT+TGG + chr5.2:48521165-48521184 None:intergenic 25.0%
! TTGATTGATCCATGTATAAA+AGG - chr5.2:48521510-48521529 MS.gene65685:intron 25.0%
!! TATGTGACCTTTTTCTAATA+AGG + chr5.2:48520380-48520399 None:intergenic 25.0%
!! TGCTATTTGAAATATGTTGT+AGG - chr5.2:48521482-48521501 MS.gene65685:intron 25.0%
!! TTAAGGCTTTATAATTGAGA+AGG + chr5.2:48521705-48521724 None:intergenic 25.0%
!! TTTCTAATAAGGCTTTAACT+AGG + chr5.2:48520369-48520388 None:intergenic 25.0%
!!! ACACATCCTTTTAATTTGAT+TGG - chr5.2:48520445-48520464 MS.gene65685:intron 25.0%
!!! GTTAAAGCCTTATTAGAAAA+AGG - chr5.2:48520370-48520389 MS.gene65685:CDS 25.0%
!!! TTATTTTGCGAAAAAACAAG+TGG + chr5.2:48520494-48520513 None:intergenic 25.0%
AATCACTTTACATAGAAGGA+TGG - chr5.2:48521038-48521057 MS.gene65685:intron 30.0%
ACCTGAATGTTGTTATCATA+TGG + chr5.2:48520680-48520699 None:intergenic 30.0%
AGATAATAATACGCATAGCA+TGG - chr5.2:48522560-48522579 MS.gene65685:intron 30.0%
AGTAATGTCACATTCTTGTT+TGG - chr5.2:48521799-48521818 MS.gene65685:intron 30.0%
ATCAAAAGCTTTCAAGACAA+AGG - chr5.2:48522651-48522670 MS.gene65685:CDS 30.0%
ATGATACTAAGCATCATCAA+AGG - chr5.2:48522134-48522153 MS.gene65685:intron 30.0%
TAAAAAATAACAGGTTCCAG+AGG - chr5.2:48522246-48522265 MS.gene65685:intron 30.0%
TAGAAAAAGGTCACATAGTA+AGG - chr5.2:48520383-48520402 MS.gene65685:CDS 30.0%
TATGCATATGCAAAGACATT+GGG - chr5.2:48521999-48522018 MS.gene65685:intron 30.0%
TCCATATGATAACAACATTC+AGG - chr5.2:48520676-48520695 MS.gene65685:CDS 30.0%
TTATCATATGGAACAATCAC+AGG + chr5.2:48520668-48520687 None:intergenic 30.0%
!! GATTTTAAAAGCAGATCTGA+TGG - chr5.2:48520583-48520602 MS.gene65685:CDS 30.0%
!!! GTCTGTTACATTAGTCTTTT+TGG - chr5.2:48521914-48521933 MS.gene65685:intron 30.0%
!!! TGTTCAAGAGTTTTGAATTG+AGG + chr5.2:48522615-48522634 None:intergenic 30.0%
AAAAAACACGACTATTACGG+CGG - chr5.2:48521258-48521277 MS.gene65685:intron 35.0%
CTCCAATGAGATTCATTAAG+AGG + chr5.2:48521642-48521661 None:intergenic 35.0%
CTCCTCTTAATGAATCTCAT+TGG - chr5.2:48521637-48521656 MS.gene65685:intron 35.0%
GCGTATTATTATCTCCTTTC+TGG + chr5.2:48522554-48522573 None:intergenic 35.0%
GGTTATCTAACTGAACTAAG+TGG - chr5.2:48520542-48520561 MS.gene65685:intron 35.0%
GTACGGATAAGAAGGATATT+TGG + chr5.2:48522421-48522440 None:intergenic 35.0%
GTATGCATATGCAAAGACAT+TGG - chr5.2:48521998-48522017 MS.gene65685:intron 35.0%
TCAAATCATCCAAATGCTTC+AGG - chr5.2:48522395-48522414 MS.gene65685:CDS 35.0%
TCACAAAAAGTTCACGCATA+TGG + chr5.2:48521102-48521121 None:intergenic 35.0%
TGAACTGAACAACAAACACA+AGG + chr5.2:48520993-48521012 None:intergenic 35.0%
TTATAGCGTTTGCAGTATTC+TGG + chr5.2:48521669-48521688 None:intergenic 35.0%
TTGATTTCGTACGGATAAGA+AGG + chr5.2:48522429-48522448 None:intergenic 35.0%
TTTAAAAGCAGATCTGATGG+TGG - chr5.2:48520586-48520605 MS.gene65685:CDS 35.0%
! AAATCCCAGTTGAGTGATTT+TGG + chr5.2:48522592-48522611 None:intergenic 35.0%
! ATTTGCAGATGATTTGGAGA+AGG - chr5.2:48520517-48520536 MS.gene65685:intron 35.0%
! TTCTGGGTTCCTTTTATACA+TGG + chr5.2:48521522-48521541 None:intergenic 35.0%
! TTTTCGCATCAAGTAACCAT+AGG - chr5.2:48521127-48521146 MS.gene65685:intron 35.0%
!! AACCGCCATTTTAAACTTTG+CGG + chr5.2:48521364-48521383 None:intergenic 35.0%
!! TACGACATTTTTGAAAACGC+CGG + chr5.2:48521331-48521350 None:intergenic 35.0%
AAAATGGCGGTTTGTAGAGA+TGG - chr5.2:48521372-48521391 MS.gene65685:CDS 40.0%
AAAGACATTGGGAGAAAGAG+AGG - chr5.2:48522010-48522029 MS.gene65685:intron 40.0%
ACAAATCCCACTGTTGTGTT+TGG + chr5.2:48522279-48522298 None:intergenic 40.0%
ACCACCAAAATCACTCAACT+GGG - chr5.2:48522585-48522604 MS.gene65685:CDS 40.0%
ACTTGTGAGTTATGCAGCTT+TGG - chr5.2:48521976-48521995 MS.gene65685:intron 40.0%
ATCCTCCATAGCCAATAAGT+GGG + chr5.2:48522325-48522344 None:intergenic 40.0%
CAAATCCCACTGTTGTGTTT+GGG + chr5.2:48522278-48522297 None:intergenic 40.0%
CAGCAATCACGTCATCTATA+TGG + chr5.2:48522302-48522321 None:intergenic 40.0%
TTGGGACACATTTCATCCTA+TGG + chr5.2:48521146-48521165 None:intergenic 40.0%
TTTCACGTTTGCTTTGATGC+AGG + chr5.2:48521556-48521575 None:intergenic 40.0%
! GATATTTGGCCTGAAGCATT+TGG + chr5.2:48522407-48522426 None:intergenic 40.0%
! TGCCGCAAAGTTTAAAATGG+CGG - chr5.2:48521359-48521378 MS.gene65685:CDS 40.0%
! TTGGATGATTTGAGACCAGT+GGG + chr5.2:48522388-48522407 None:intergenic 40.0%
! TTTGGATGATTTGAGACCAG+TGG + chr5.2:48522389-48522408 None:intergenic 40.0%
!! CATTTTTGAAAACGCCGGAA+TGG + chr5.2:48521326-48521345 None:intergenic 40.0%
AACGCCGGAATGGTTTATAG+TGG + chr5.2:48521316-48521335 None:intergenic 45.0%
AGGACTACAGTAAGAAACCC+AGG - chr5.2:48520403-48520422 MS.gene65685:CDS 45.0%
AGTGGACCAACAACGAAAGA+GGG + chr5.2:48522083-48522102 None:intergenic 45.0%
ATCATGAGCAATGTGCTAGC+TGG + chr5.2:48522119-48522138 None:intergenic 45.0%
CAAAGCAAACGTGAAACGTG+TGG - chr5.2:48521560-48521579 MS.gene65685:intron 45.0%
CACCACCAAAATCACTCAAC+TGG - chr5.2:48522584-48522603 MS.gene65685:CDS 45.0%
GAGTACCCAAACACAACAGT+GGG - chr5.2:48522270-48522289 MS.gene65685:intron 45.0%
GATCCTCCATAGCCAATAAG+TGG + chr5.2:48522326-48522345 None:intergenic 45.0%
GCCACCACTATAAACCATTC+CGG - chr5.2:48521309-48521328 MS.gene65685:intron 45.0%
GCCTGCCGCAAAGTTTAAAA+TGG - chr5.2:48521356-48521375 MS.gene65685:CDS 45.0%
GTTAACCCCTCTTTCGTTGT+TGG - chr5.2:48522074-48522093 MS.gene65685:intron 45.0%
TATAGGTGTAGCAGCCAGAA+AGG - chr5.2:48522537-48522556 MS.gene65685:intron 45.0%
! AGAGTACCCAAACACAACAG+TGG - chr5.2:48522269-48522288 MS.gene65685:intron 45.0%
! GCAGATGATTTGGAGAAGGT+TGG - chr5.2:48520521-48520540 MS.gene65685:intron 45.0%
! TCCCAGTTGAGTGATTTTGG+TGG + chr5.2:48522589-48522608 None:intergenic 45.0%
!! AAACTTGTTGCACATCGTCG+CGG + chr5.2:48521617-48521636 None:intergenic 45.0%
!! AAAGCAGATCTGATGGTGGA+AGG - chr5.2:48520590-48520609 MS.gene65685:CDS 45.0%
!! AAGCAGATCTGATGGTGGAA+GGG - chr5.2:48520591-48520610 MS.gene65685:CDS 45.0%
!! AGTTTTGATGAGGCAGTGAC+TGG - chr5.2:48520614-48520633 MS.gene65685:CDS 45.0%
!! GCCATTTTAAACTTTGCGGC+AGG + chr5.2:48521360-48521379 None:intergenic 45.0%
ACAAGCCTGCCAGATGCTTT+GGG + chr5.2:48522348-48522367 None:intergenic 50.0%
CACATCGTCGCGGTATCTTA+TGG + chr5.2:48521607-48521626 None:intergenic 50.0%
CATTGGGAGAAAGAGAGGCT+TGG - chr5.2:48522015-48522034 MS.gene65685:intron 50.0%
CGTGATTGCTGCCCACTTAT+TGG - chr5.2:48522311-48522330 MS.gene65685:CDS 50.0%
GAGTGGACCAACAACGAAAG+AGG + chr5.2:48522084-48522103 None:intergenic 50.0%
GCAGGAGTTCAGCACATTCT+GGG + chr5.2:48521538-48521557 None:intergenic 50.0%
GCCGGAATGGTTTATAGTGG+TGG + chr5.2:48521313-48521332 None:intergenic 50.0%
GGAGGATTGCAGAGGAAAGT+GGG - chr5.2:48522036-48522055 MS.gene65685:intron 50.0%
GTGGACCAACAACGAAAGAG+GGG + chr5.2:48522082-48522101 None:intergenic 50.0%
TACAAGCCTGCCAGATGCTT+TGG + chr5.2:48522349-48522368 None:intergenic 50.0%
TGCAGGAGTTCAGCACATTC+TGG + chr5.2:48521539-48521558 None:intergenic 50.0%
TGGAGGATTGCAGAGGAAAG+TGG - chr5.2:48522035-48522054 MS.gene65685:intron 50.0%
TGTGTTTGGGTACTCTCCTC+TGG + chr5.2:48522265-48522284 None:intergenic 50.0%
! ATGCCTGAGTTTTGCGTGAC+AGG - chr5.2:48520319-48520338 MS.gene65685:CDS 50.0%
! ATGGAGGATCCCAAAGCATC+TGG - chr5.2:48522336-48522355 MS.gene65685:CDS 50.0%
! GGAAAGTGGGCTTGATCTAG+TGG - chr5.2:48522049-48522068 MS.gene65685:intron 50.0%
! TGCCCACTTATTGGCTATGG+AGG - chr5.2:48522320-48522339 MS.gene65685:CDS 50.0%
! TGCTGCCCACTTATTGGCTA+TGG - chr5.2:48522317-48522336 MS.gene65685:CDS 50.0%
!! GGTGGAAGGGAGTTTTGATG+AGG - chr5.2:48520604-48520623 MS.gene65685:CDS 50.0%
AGAGGCTTGGAGGATTGCAG+AGG - chr5.2:48522028-48522047 MS.gene65685:intron 55.0%
AGCAATGTGCTAGCTGGCTG+TGG + chr5.2:48522113-48522132 None:intergenic 55.0%
CCTCCTGTCACGCAAAACTC+AGG + chr5.2:48520325-48520344 None:intergenic 55.0%
GTTCAACCACAGTTGCCCAC+TGG - chr5.2:48522370-48522389 MS.gene65685:CDS 55.0%
TGGGAGAAAGAGAGGCTTGG+AGG - chr5.2:48522018-48522037 MS.gene65685:intron 55.0%
TTGAGACCAGTGGGCAACTG+TGG + chr5.2:48522379-48522398 None:intergenic 55.0%
! AGGATCCCAAAGCATCTGGC+AGG - chr5.2:48522340-48522359 MS.gene65685:CDS 55.0%
! CCTGAGTTTTGCGTGACAGG+AGG - chr5.2:48520322-48520341 MS.gene65685:CDS 55.0%
! GAGGCAGTGACTGGTGTTGA+TGG - chr5.2:48520623-48520642 MS.gene65685:CDS 55.0%
!! TTTGCGTGACAGGAGGCACT+GGG - chr5.2:48520329-48520348 MS.gene65685:CDS 55.0%
!! TTTTGCGTGACAGGAGGCAC+TGG - chr5.2:48520328-48520347 MS.gene65685:CDS 55.0%
TGCTAGCTGGCTGTGGTGCA+AGG + chr5.2:48522106-48522125 None:intergenic 60.0%
GCTGGCTGTGGTGCAAGGAG+TGG + chr5.2:48522101-48522120 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 48520319 48522683 48520319 ID=MS.gene65685
chr5.2 mRNA 48520319 48522683 48520319 ID=MS.gene65685.t1;Parent=MS.gene65685
chr5.2 exon 48522578 48522683 48522578 ID=MS.gene65685.t1.exon1;Parent=MS.gene65685.t1
chr5.2 CDS 48522578 48522683 48522578 ID=cds.MS.gene65685.t1;Parent=MS.gene65685.t1
chr5.2 exon 48522305 48522477 48522305 ID=MS.gene65685.t1.exon2;Parent=MS.gene65685.t1
chr5.2 CDS 48522305 48522477 48522305 ID=cds.MS.gene65685.t1;Parent=MS.gene65685.t1
chr5.2 exon 48521313 48521498 48521313 ID=MS.gene65685.t1.exon3;Parent=MS.gene65685.t1
chr5.2 CDS 48521313 48521498 48521313 ID=cds.MS.gene65685.t1;Parent=MS.gene65685.t1
chr5.2 exon 48520847 48521009 48520847 ID=MS.gene65685.t1.exon4;Parent=MS.gene65685.t1
chr5.2 CDS 48520847 48521009 48520847 ID=cds.MS.gene65685.t1;Parent=MS.gene65685.t1
chr5.2 exon 48520554 48520743 48520554 ID=MS.gene65685.t1.exon5;Parent=MS.gene65685.t1
chr5.2 CDS 48520554 48520743 48520554 ID=cds.MS.gene65685.t1;Parent=MS.gene65685.t1
chr5.2 exon 48520319 48520460 48520319 ID=MS.gene65685.t1.exon6;Parent=MS.gene65685.t1
chr5.2 CDS 48520319 48520460 48520319 ID=cds.MS.gene65685.t1;Parent=MS.gene65685.t1
Gene Sequence

>MS.gene65685

ATGCCTGAGTTTTGCGTGACAGGAGGCACTGGGTTCATAGCAGCTTACCTAGTTAAAGCCTTATTAGAAAAAGGTCACATAGTAAGGACTACAGTAAGAAACCCAGGTATTAATATTTAGTGATATACACATCCTTTTAATTTGATTGGTACATTAATTTTTCATTTTTAATCCACTTGTTTTTTCGCAAAATAAATAATTTGCAGATGATTTGGAGAAGGTTGGTTATCTAACTGAACTAAGTGGAGATAGAGAAAGATTAAAGATTTTAAAAGCAGATCTGATGGTGGAAGGGAGTTTTGATGAGGCAGTGACTGGTGTTGATGGTGTGTTTCATACAGCATCTCCTGTGATTGTTCCATATGATAACAACATTCAGGTACACTTTTCTATTTCTTATAAAAGTGTGTAATTAATTTTGAATTTTAGTTTTAATTTGAGAAATGATATTTATACAATTATTTTGGGATAACTTTTGTGACAAATTTCTGTCTCTATTCGTTGATTTCCGTATCAATACTTAATTTTTATGTAAATTTACTGTTGTCGCACAAATTATTGTTCAAATAACACACCACTTTTAATTTTTCTGTCAAAATTAAATAATTATGAATTGTAGTTTTTATTTTGCTTTCGAAATTGTTGTGTTTGATGTCTAGAGTTGATTTTAACCTTGTGTTTGTTGTTCAGTTCACTTTTCATAAATACAGCGAAACATAAATCACTTTACATAGAAGGATGGTTGCAAATAAAAATGATGAGCTGCTACTGTTACAGTAACCATATGCGTGAACTTTTTGTGAATTATTTTTCGCATCAAGTAACCATAGGATGAAATGTGTCCCAAATATTTAGTTACAGAGAGAAATTGATGTGAGACTACTTTGTAAATTCAGAAAGTAATTCAATAAAAAATTGTCTTTGTTAATAGACACTACAAAAAAACACGACTATTACGGCGGATAAAATTTGCATATTAAGGCAGTTTTAGCCACCACTATAAACCATTCCGGCGTTTTCAAAAATGTCGTAATTTTGCCTGCCGCAAAGTTTAAAATGGCGGTTTGTAGAGATGGTATATATTTTCATCTTTTCAATAACATAACATCATCAATATACCTCTTCAAAAAAAAAACATCATCAATATAGATATGGTAATTTGTTGCTATTTGAAATATGTTGTAGGCTACTTTGATTGATCCATGTATAAAAGGAACCCAGAATGTGCTGAACTCCTGCATCAAAGCAAACGTGAAACGTGTGGTGTTAACATCTTCTTGCTCTTCCATAAGATACCGCGACGATGTGCAACAAGTTTCTCCTCTTAATGAATCTCATTGGAGTGATCCAGAATACTGCAAACGCTATAATGTAACAACACTTCCTTCTCAATTATAAAGCCTTAAGTCATATCAAATTCTTGTTTGGATCATCACTTTGAGTAATATAGTTTACAAGAACTACATATATCATCACTTTAAGTAATGTCACATTCTTGTTTGGATCATAACTTTGAATTTTATGTTTTCTTTTTCAATATTTTTGAATCATTTTGAAACTAACCAAATTTTAAAATAATACTCAAACTAAAAATAGTCTGTTACATTAGTCTTTTTGGATCAATCAATTTAAATTAGTGTAATTTAAATTCAATGAAACTTGTGAGTTATGCAGCTTTGGTATGCATATGCAAAGACATTGGGAGAAAGAGAGGCTTGGAGGATTGCAGAGGAAAGTGGGCTTGATCTAGTGGTTGTTAACCCCTCTTTCGTTGTTGGTCCACTCCTTGCACCACAGCCAGCTAGCACATTGCTCATGATACTAAGCATCATCAAAGGTTATATAATTCTCTCTCAAATGTGTTTTGATGTTAAGAATTCTTTGTATTCTATACCATATTTCTTATGACATTAAGTGTTATTTAATCTAAAAAATAACAGGTTCCAGAGGAGAGTACCCAAACACAACAGTGGGATTTGTCCATATAGATGACGTGATTGCTGCCCACTTATTGGCTATGGAGGATCCCAAAGCATCTGGCAGGCTTGTATGTTCAACCACAGTTGCCCACTGGTCTCAAATCATCCAAATGCTTCAGGCCAAATATCCTTCTTATCCGTACGAAATCAAGTAAGTTTAAGAATTGCTGTATATCATATACTATATCAACCTTAATTTTGATATTATATAGAAAAAGTATTTATACTTTCTTTTCTACTATAGGTGTAGCAGCCAGAAAGGAGATAATAATACGCATAGCATGGACACCACCAAAATCACTCAACTGGGATTTCCTCAATTCAAAACTCTTGAACAAATGTTTGATGACTGCATCAAAAGCTTTCAAGACAAAGGCTTCCTATGA
Protein sequence

>MS.gene65685.t1

MPEFCVTGGTGFIAAYLVKALLEKGHIVRTTVRNPDDLEKVGYLTELSGDRERLKILKADLMVEGSFDEAVTGVDGVFHTASPVIVPYDNNIQATLIDPCIKGTQNVLNSCIKANVKRVVLTSSCSSIRYRDDVQQVSPLNESHWSDPEYCKRYNLWYAYAKTLGEREAWRIAEESGLDLVVVNPSFVVGPLLAPQPASTLLMILSIIKGSRGEYPNTTVGFVHIDDVIAAHLLAMEDPKASGRLVCSTTVAHWSQIIQMLQAKYPSYPYEIKCSSQKGDNNTHSMDTTKITQLGFPQFKTLEQMFDDCIKSFQDKGFL