AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96654

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96654.t1 MTR_5g056600 97.492 319 8 0 1 319 1 319 0.0 652
MS.gene96654.t1 MTR_3g005210 41.325 317 181 5 5 319 7 320 1.64e-85 260
MS.gene96654.t1 MTR_8g062440 41.693 319 176 6 5 319 9 321 7.71e-83 253
MS.gene96654.t1 MTR_2g101330 41.956 317 179 4 5 319 6 319 1.19e-82 253
MS.gene96654.t1 MTR_3g005170 41.009 317 180 6 5 318 11 323 1.20e-82 253
MS.gene96654.t1 MTR_8g062110 42.722 316 171 6 5 316 10 319 5.25e-82 251
MS.gene96654.t1 MTR_8g062110 42.722 316 171 6 5 316 19 328 6.92e-82 251
MS.gene96654.t1 MTR_8g062440 41.562 320 176 7 5 319 9 322 6.93e-81 248
MS.gene96654.t1 MTR_3g005170 44.737 266 141 5 5 267 11 273 3.97e-78 240
MS.gene96654.t1 MTR_8g062440 43.051 295 159 5 5 295 9 298 1.12e-76 238
MS.gene96654.t1 MTR_8g062110 46.067 267 135 5 5 267 10 271 1.58e-76 236
MS.gene96654.t1 MTR_8g062110 46.067 267 135 5 5 267 10 271 2.04e-76 236
MS.gene96654.t1 MTR_2g104960 43.082 318 171 7 4 319 20 329 7.07e-75 234
MS.gene96654.t1 MTR_4g006940 42.089 316 175 5 5 319 18 326 6.05e-74 231
MS.gene96654.t1 MTR_1g022445 38.125 320 190 6 5 319 9 325 4.25e-72 226
MS.gene96654.t1 MTR_1g022440 37.500 320 192 6 5 319 9 325 1.80e-71 225
MS.gene96654.t1 MTR_4g077130 39.241 316 187 5 5 318 10 322 5.07e-71 223
MS.gene96654.t1 MTR_4g077100 39.748 317 184 6 5 318 12 324 8.38e-71 223
MS.gene96654.t1 MTR_5g072620 38.438 320 186 5 5 319 6 319 7.03e-70 220
MS.gene96654.t1 MTR_4g009040 36.449 321 195 5 3 319 12 327 4.53e-68 216
MS.gene96654.t1 MTR_4g009040 36.250 320 193 5 3 319 12 323 3.80e-67 213
MS.gene96654.t1 MTR_3g005170 35.759 316 167 5 5 318 11 292 1.96e-66 210
MS.gene96654.t1 MTR_4g006940 44.238 269 144 3 5 273 18 280 3.56e-66 209
MS.gene96654.t1 MTR_4g006940 44.238 269 144 3 5 273 18 280 3.56e-66 209
MS.gene96654.t1 MTR_4g077100 41.729 266 149 5 5 267 12 274 8.82e-65 206
MS.gene96654.t1 MTR_7g074880 35.093 322 198 7 5 319 9 326 7.82e-64 205
MS.gene96654.t1 MTR_3g031650 33.544 316 207 3 5 319 6 319 5.89e-63 202
MS.gene96654.t1 MTR_4g081440 37.500 336 187 8 3 319 23 354 2.00e-60 197
MS.gene96654.t1 MTR_3g031650 36.364 264 168 0 5 268 6 269 2.39e-60 194
MS.gene96654.t1 MTR_7g074730 36.111 324 194 8 5 319 9 328 3.26e-60 196
MS.gene96654.t1 MTR_7g074820 36.923 325 191 8 5 319 9 329 3.82e-60 195
MS.gene96654.t1 MTR_4g081440 37.202 336 187 8 3 319 19 349 4.04e-60 196
MS.gene96654.t1 MTR_4g009690 35.000 320 197 5 3 319 12 323 3.73e-59 192
MS.gene96654.t1 MTR_4g009690 35.312 320 195 6 3 319 12 322 5.17e-59 192
MS.gene96654.t1 MTR_7g074850 33.846 325 201 8 5 319 9 329 3.15e-54 180
MS.gene96654.t1 MTR_7g074710 33.945 327 195 7 5 319 9 326 6.63e-54 179
MS.gene96654.t1 MTR_4g092080 31.707 328 203 9 5 317 15 336 1.50e-52 176
MS.gene96654.t1 MTR_8g062110 37.281 228 134 5 93 316 11 233 1.64e-46 157
MS.gene96654.t1 MTR_2g028620 30.938 320 212 4 5 319 11 326 3.75e-46 159
MS.gene96654.t1 MTR_7g074870 32.069 290 187 6 36 319 3 288 1.04e-44 154
MS.gene96654.t1 MTR_2g028620 33.704 270 171 3 5 269 11 277 1.09e-44 155
MS.gene96654.t1 MTR_4g023730 30.864 324 212 7 5 319 9 329 1.65e-44 155
MS.gene96654.t1 MTR_8g062110 40.782 179 98 4 93 267 11 185 6.87e-41 141
MS.gene96654.t1 MTR_2g013250 28.783 337 209 9 5 317 8 337 1.71e-33 126
MS.gene96654.t1 MTR_2g013230 28.739 341 208 10 5 319 9 340 3.67e-32 122
MS.gene96654.t1 MTR_6g406250 31.183 279 175 9 5 274 56 326 4.90e-30 117
MS.gene96654.t1 MTR_2g013260 27.246 345 204 9 5 317 9 338 2.76e-29 115
MS.gene96654.t1 MTR_7g117190 28.107 338 215 12 5 319 8 340 3.62e-29 114
MS.gene96654.t1 MTR_5g029990 26.400 250 158 7 5 247 13 243 2.65e-28 111
MS.gene96654.t1 MTR_0164s0070 43.750 128 71 1 3 129 4 131 2.33e-27 106
MS.gene96654.t1 MTR_7g117190 28.772 285 181 10 5 268 8 291 3.21e-26 105
MS.gene96654.t1 MTR_0685s0030 33.742 163 106 2 156 318 1 161 5.18e-26 101
MS.gene96654.t1 MTR_2g013260 27.703 296 169 8 5 268 9 291 7.11e-26 104
MS.gene96654.t1 MTR_6g406250 32.227 211 133 6 5 208 56 263 7.51e-26 103
MS.gene96654.t1 MTR_0685s0030 35.714 112 71 1 156 267 1 111 6.62e-20 84.0
MS.gene96654.t1 MTR_3g011130 38.793 116 68 3 96 208 30 145 1.09e-19 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96654.t1 AT1G68540 73.520 321 83 1 1 319 1 321 0.0 508
MS.gene96654.t1 AT1G25460 64.174 321 111 3 1 319 1 319 1.82e-152 430
MS.gene96654.t1 AT1G68540 73.832 214 54 1 108 319 1 214 3.51e-121 347
MS.gene96654.t1 AT4G35420 47.634 317 160 5 5 319 9 321 2.95e-98 293
MS.gene96654.t1 AT1G51410 41.824 318 178 6 5 319 10 323 3.07e-84 257
MS.gene96654.t1 AT5G19440 41.456 316 180 5 5 318 11 323 1.99e-81 250
MS.gene96654.t1 AT1G09510 40.446 314 182 5 5 316 9 319 1.53e-73 229
MS.gene96654.t1 AT5G58490 36.677 319 193 4 5 319 10 323 1.46e-67 214
MS.gene96654.t1 AT1G15950 39.873 316 182 5 5 319 14 322 2.42e-67 214
MS.gene96654.t1 AT1G15950 39.873 316 182 5 5 319 14 322 2.42e-67 214
MS.gene96654.t1 AT5G42800 37.578 322 189 8 5 319 9 325 2.54e-66 213
MS.gene96654.t1 AT1G09480 36.909 317 195 5 5 319 9 322 4.93e-66 210
MS.gene96654.t1 AT1G09480 36.909 317 195 5 5 319 56 369 1.30e-65 211
MS.gene96654.t1 AT1G09490 36.677 319 193 6 5 319 9 322 1.72e-65 209
MS.gene96654.t1 AT1G80820 38.924 316 185 4 5 319 9 317 7.90e-65 207
MS.gene96654.t1 AT1G09500 35.714 322 189 6 5 316 9 322 2.49e-64 206
MS.gene96654.t1 AT1G15950 39.145 304 178 4 5 307 14 311 5.31e-63 203
MS.gene96654.t1 AT1G66800 37.423 326 177 9 5 319 9 318 1.41e-62 201
MS.gene96654.t1 AT1G09500 39.098 266 155 4 5 266 9 271 6.94e-62 198
MS.gene96654.t1 AT2G02400 31.861 317 209 5 5 319 7 318 7.19e-60 194
MS.gene96654.t1 AT1G15950 41.264 269 152 3 5 273 14 276 2.65e-59 191
MS.gene96654.t1 AT1G15950 41.509 265 149 3 5 269 14 272 5.95e-59 191
MS.gene96654.t1 AT1G15950 41.509 265 149 3 5 269 14 272 8.70e-59 191
MS.gene96654.t1 AT2G33590 36.013 311 193 4 5 314 10 315 1.51e-57 188
MS.gene96654.t1 AT1G80820 39.405 269 157 2 5 273 9 271 1.37e-55 182
MS.gene96654.t1 AT4G27250 33.133 332 180 9 4 310 13 327 7.50e-52 174
MS.gene96654.t1 AT1G09500 34.130 293 175 6 34 316 4 288 8.49e-52 172
MS.gene96654.t1 AT4G27250 33.235 340 179 10 4 310 13 337 1.05e-51 174
MS.gene96654.t1 AT1G09490 34.722 288 179 6 36 319 9 291 1.48e-51 172
MS.gene96654.t1 AT2G33600 34.505 313 199 4 3 314 8 315 6.39e-51 171
MS.gene96654.t1 AT1G61720 33.233 331 198 7 5 319 14 337 1.30e-50 171
MS.gene96654.t1 AT2G45400 32.822 326 206 8 5 319 41 364 2.61e-47 163
MS.gene96654.t1 AT1G76470 34.591 318 202 4 5 319 8 322 3.09e-47 162
MS.gene96654.t1 AT2G45400 34.307 274 169 6 5 268 41 313 1.38e-43 152
MS.gene96654.t1 AT1G15950 35.556 225 143 2 96 319 1 224 8.28e-40 140
MS.gene96654.t1 AT2G45400 30.903 288 188 7 41 319 22 307 8.41e-35 129
MS.gene96654.t1 AT2G45400 34.722 216 131 5 5 211 41 255 2.20e-31 118
MS.gene96654.t1 AT2G23910 25.830 271 184 7 5 269 11 270 3.58e-30 115
MS.gene96654.t1 AT2G23910 25.830 271 184 7 5 269 11 270 3.59e-30 115
MS.gene96654.t1 AT2G23910 25.830 271 184 7 5 269 11 270 5.36e-30 116
MS.gene96654.t1 AT4G30470 26.119 268 186 6 5 269 11 269 1.82e-27 109
MS.gene96654.t1 AT5G14700 29.588 267 155 7 5 251 56 309 2.66e-27 110
MS.gene96654.t1 AT2G23910 26.724 232 153 7 44 269 16 236 5.12e-25 102
MS.gene96654.t1 AT5G14700 31.280 211 132 4 5 205 56 263 2.96e-23 97.4

Find 71 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGTATTATTATCTCCTTTC+TGG 0.077666 5.3:+45501162 None:intergenic
CGTGATTGCTGCCCACTTAT+TGG 0.174389 5.3:-45501402 MS.gene96654:CDS
TTGATTGATCCATGTATAAA+AGG 0.233674 5.3:-45502203 MS.gene96654:CDS
TGCAGGAGTTCAGCACATTC+TGG 0.245081 5.3:+45502177 None:intergenic
TTATAGCGTTTGCAGTATTC+TGG 0.245242 5.3:+45502047 None:intergenic
CACATCGTCGCGGTATCTTA+TGG 0.246307 5.3:+45502109 None:intergenic
GTACGGATAAGAAGGATATT+TGG 0.250292 5.3:+45501295 None:intergenic
AAATTAATTTGCAGATGATT+TGG 0.284567 5.3:-45503014 MS.gene96654:intron
GTTAAAGCCTTATTAGAAAA+AGG 0.298250 5.3:-45503159 MS.gene96654:CDS
CAGCAATCACGTCATCTATA+TGG 0.329414 5.3:+45501414 None:intergenic
TCCATATGATAACAACATTC+AGG 0.350735 5.3:-45502849 MS.gene96654:intron
GATATTTGGCCTGAAGCATT+TGG 0.356020 5.3:+45501309 None:intergenic
GTTAACCCCTCTTTCGTTGT+TGG 0.364774 5.3:-45501639 MS.gene96654:CDS
TACAAGCCTGCCAGATGCTT+TGG 0.370462 5.3:+45501367 None:intergenic
TGCTGCCCACTTATTGGCTA+TGG 0.389523 5.3:-45501396 MS.gene96654:CDS
TCAAATCATCCAAATGCTTC+AGG 0.391082 5.3:-45501318 MS.gene96654:CDS
CTCCAATGAGATTCATTAAG+AGG 0.392826 5.3:+45502074 None:intergenic
CTCCTCTTAATGAATCTCAT+TGG 0.405823 5.3:-45502076 MS.gene96654:CDS
TGTGTTTGGGTACTCTCCTC+TGG 0.407140 5.3:+45501451 None:intergenic
ACAAATCCCACTGTTGTGTT+TGG 0.410589 5.3:+45501437 None:intergenic
ACCTGAATGTTGTTATCATA+TGG 0.449261 5.3:+45502848 None:intergenic
TTGATTTCGTACGGATAAGA+AGG 0.451640 5.3:+45501287 None:intergenic
CAAATCCCACTGTTGTGTTT+GGG 0.454369 5.3:+45501438 None:intergenic
GCAGGAGTTCAGCACATTCT+GGG 0.454583 5.3:+45502178 None:intergenic
GAGGCAGTGACTGGGGTTGA+TGG 0.459132 5.3:-45502902 MS.gene96654:CDS
GCAGATGATTTGGAGAAGGT+TGG 0.460715 5.3:-45503004 MS.gene96654:intron
ATGGAGGAGTCCAAAGCATC+TGG 0.477461 5.3:-45501377 MS.gene96654:CDS
TTATCATATGGAACAATCAC+AGG 0.479329 5.3:+45502860 None:intergenic
TAAACTTACTTGATTTCGTA+CGG 0.486707 5.3:+45501278 None:intergenic
AGCAATGTGCTAGCTGGCTG+TGG 0.489492 5.3:+45501603 None:intergenic
TGGGAGAAAGAGAGGCTTGG+AGG 0.506920 5.3:-45501695 MS.gene96654:CDS
TTTCTAATAAGGCTTTAACT+AGG 0.507635 5.3:+45503163 None:intergenic
TATAGGTGTAGCAGCCAGAA+AGG 0.509846 5.3:-45501176 MS.gene96654:intron
TATGCATATGCAAAGACATT+GGG 0.522633 5.3:-45501714 MS.gene96654:CDS
TTTCACGTTTGCTTTGATGC+AGG 0.527457 5.3:+45502160 None:intergenic
TGGAGGATTGCAGAGGAAAG+TGG 0.531373 5.3:-45501678 MS.gene96654:CDS
ATCAAAAGCTTTCAAGACAA+AGG 0.539091 5.3:-45501062 MS.gene96654:CDS
ATTTGCAGATGATTTGGAGA+AGG 0.544790 5.3:-45503008 MS.gene96654:intron
GGAAAGTGGGCTTGATCTAG+TGG 0.545333 5.3:-45501664 MS.gene96654:CDS
ACTCCTCCATAGCCAATAAG+TGG 0.545932 5.3:+45501390 None:intergenic
AAGTGGACCAACAACGAAAG+AGG 0.547830 5.3:+45501632 None:intergenic
ATCATGAGCAATGTGCTAGC+TGG 0.548471 5.3:+45501597 None:intergenic
CATTGGGAGAAAGAGAGGCT+TGG 0.548941 5.3:-45501698 MS.gene96654:CDS
CCTCCTGTCACGCAAAACTC+AGG 0.555398 5.3:+45503207 None:intergenic
TTGGATGATTTGAGACCAGT+GGG 0.557451 5.3:+45501328 None:intergenic
TTTGCGTGACAGGAGGCACT+GGG 0.559306 5.3:-45503200 MS.gene96654:CDS
AGTGGACCAACAACGAAAGA+GGG 0.561902 5.3:+45501633 None:intergenic
GGAGGATTGCAGAGGAAAGT+GGG 0.562235 5.3:-45501677 MS.gene96654:CDS
TGCCCACTTATTGGCTATGG+AGG 0.564412 5.3:-45501393 MS.gene96654:CDS
GCTGGCTGTGGTGCAAGAAG+TGG 0.567315 5.3:+45501615 None:intergenic
AAGCAGATCTGATGGTGGAA+GGG 0.569525 5.3:-45502934 MS.gene96654:CDS
AAAGCAGATCTGATGGTGGA+AGG 0.575224 5.3:-45502935 MS.gene96654:CDS
TTTGGATGATTTGAGACCAG+TGG 0.578890 5.3:+45501327 None:intergenic
AGGAGTCCAAAGCATCTGGC+AGG 0.581188 5.3:-45501373 MS.gene96654:CDS
GTTCAACCACAGTTGCCCAC+TGG 0.588605 5.3:-45501343 MS.gene96654:CDS
AGATAATAATACGCATAGCA+TGG 0.591714 5.3:-45501153 MS.gene96654:CDS
GTATGCATATGCAAAGACAT+TGG 0.592386 5.3:-45501715 MS.gene96654:CDS
GAGTACCCAAACACAACAGT+GGG 0.606732 5.3:-45501443 MS.gene96654:CDS
AAAGACATTGGGAGAAAGAG+AGG 0.610985 5.3:-45501703 MS.gene96654:CDS
TTGAGACCAGTGGGCAACTG+TGG 0.613180 5.3:+45501337 None:intergenic
ATGATACTAAGCATCGTCAA+AGG 0.630926 5.3:-45501579 MS.gene96654:intron
CTCCTCCATAGCCAATAAGT+GGG 0.636852 5.3:+45501391 None:intergenic
TTTAAAAGCAGATCTGATGG+TGG 0.645545 5.3:-45502939 MS.gene96654:CDS
CAAAGCAAACGTGAAACGTG+TGG 0.647352 5.3:-45502153 MS.gene96654:CDS
AAACTTGTTGCACATCGTCG+CGG 0.647555 5.3:+45502099 None:intergenic
AGAGGCTTGGAGGATTGCAG+AGG 0.653350 5.3:-45501685 MS.gene96654:CDS
AGAACTACAGTGAGAAACCC+AGG 0.654416 5.3:-45503126 MS.gene96654:intron
TATCATATGGAACAATCACA+GGG 0.665757 5.3:+45502861 None:intergenic
GTGGACCAACAACGAAAGAG+GGG 0.669528 5.3:+45501634 None:intergenic
AGAGTACCCAAACACAACAG+TGG 0.693456 5.3:-45501444 MS.gene96654:CDS
ATCATATGGAACAATCACAG+GGG 0.784728 5.3:+45502862 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTAATCTAAAAAATAAC+AGG - chr5.3:45502786-45502805 MS.gene96654:intron 10.0%
!! TCTATATAATATCAAAATTA+AGG + chr5.3:45503040-45503059 None:intergenic 10.0%
!!! GATATTTATACAATTATTTT+GGG - chr5.3:45501501-45501520 MS.gene96654:intron 10.0%
!!! TGATATTTATACAATTATTT+TGG - chr5.3:45501500-45501519 MS.gene96654:intron 10.0%
!!! TTGAGTATTATTTTAAAATT+TGG + chr5.3:45502433-45502452 None:intergenic 10.0%
!! GATAATTTAATAATCTAACA+AGG - chr5.3:45501790-45501809 MS.gene96654:intron 15.0%
!!! ATAAAAACTAAGTATTGATA+CGG + chr5.3:45501568-45501587 None:intergenic 15.0%
!! AATGTACCAATCAAATTAAA+AGG + chr5.3:45501187-45501206 None:intergenic 20.0%
!! ACTTAATGTCATAAGAAATA+TGG + chr5.3:45502764-45502783 None:intergenic 20.0%
!! ATCAGTAAATATTAATACCT+GGG + chr5.3:45501156-45501175 None:intergenic 20.0%
!! TATCAGTAAATATTAATACC+TGG + chr5.3:45501157-45501176 None:intergenic 20.0%
!! TTTGAAAGCAAAATTAAAAG+TGG + chr5.3:45501646-45501665 None:intergenic 20.0%
!!! AAATTAATTTGCAGATGATT+TGG - chr5.3:45501248-45501267 MS.gene96654:intron 20.0%
!!! ATACATCCTTTTAATTTGAT+TGG - chr5.3:45501178-45501197 MS.gene96654:intron 20.0%
!!! TATACTTTCTTTTCTACTAT+AGG - chr5.3:45503069-45503088 MS.gene96654:intron 20.0%
!!! TTCTTTTTTGAATATGTGTA+AGG + chr5.3:45501731-45501750 None:intergenic 20.0%
! AGCATCATCAATATAACATA+TGG - chr5.3:45502000-45502019 MS.gene96654:intron 25.0%
! AGTCATATCAAATTCTTGTT+TGG - chr5.3:45502273-45502292 MS.gene96654:intron 25.0%
! CAAGAATTTGATATGACTTA+AGG + chr5.3:45502271-45502290 None:intergenic 25.0%
! TAAACTTACTTGATTTCGTA+CGG + chr5.3:45502987-45503006 None:intergenic 25.0%
! TTGATTGATCCATGTATAAA+AGG - chr5.3:45502059-45502078 MS.gene96654:CDS 25.0%
!! TATGTGACCTTTTTCTAATA+AGG + chr5.3:45501113-45501132 None:intergenic 25.0%
!! TGCTATTTGAAATATGTTGT+AGG - chr5.3:45502031-45502050 MS.gene96654:intron 25.0%
!! TTAAGGCTTTATAATTGAGA+AGG + chr5.3:45502254-45502273 None:intergenic 25.0%
!! TTTCTAATAAGGCTTTAACT+AGG + chr5.3:45501102-45501121 None:intergenic 25.0%
!!! GTTAAAGCCTTATTAGAAAA+AGG - chr5.3:45501103-45501122 MS.gene96654:CDS 25.0%
!!! TTATTTTGCGAAAAAACAAG+TGG + chr5.3:45501227-45501246 None:intergenic 25.0%
ACCTGAATGTTGTTATCATA+TGG + chr5.3:45501417-45501436 None:intergenic 30.0%
AGATAATAATACGCATAGCA+TGG - chr5.3:45503109-45503128 MS.gene96654:intron 30.0%
AGTAATGTCACATTCTTGTT+TGG - chr5.3:45502348-45502367 MS.gene96654:intron 30.0%
ATCAAAAGCTTTCAAGACAA+AGG - chr5.3:45503200-45503219 MS.gene96654:CDS 30.0%
GATCGTTAGATATTGAGATT+AGG - chr5.3:45501845-45501864 MS.gene96654:intron 30.0%
TAAAAAATAACAGGTTCCAG+AGG - chr5.3:45502795-45502814 MS.gene96654:intron 30.0%
TATCATATGGAACAATCACA+GGG + chr5.3:45501404-45501423 None:intergenic 30.0%
TATGCATATGCAAAGACATT+GGG - chr5.3:45502548-45502567 MS.gene96654:intron 30.0%
TCCATATGATAACAACATTC+AGG - chr5.3:45501413-45501432 MS.gene96654:CDS 30.0%
TTATCATATGGAACAATCAC+AGG + chr5.3:45501405-45501424 None:intergenic 30.0%
!! GATTTTAAAAGCAGATCTGA+TGG - chr5.3:45501320-45501339 MS.gene96654:CDS 30.0%
!!! GTCTGTTACATTAGTCTTTT+TGG - chr5.3:45502463-45502482 MS.gene96654:intron 30.0%
!!! TGTTCAAGAGTTTTGAATTG+AGG + chr5.3:45503164-45503183 None:intergenic 30.0%
AACATCACAACACATTTGAG+AGG + chr5.3:45502720-45502739 None:intergenic 35.0%
AATTGTGAGTTATGCAGCTT+TGG - chr5.3:45502525-45502544 MS.gene96654:intron 35.0%
ACATCACAACACATTTGAGA+GGG + chr5.3:45502719-45502738 None:intergenic 35.0%
ACCACAAAAATCACTCAACT+GGG - chr5.3:45503134-45503153 MS.gene96654:CDS 35.0%
ATCATATGGAACAATCACAG+GGG + chr5.3:45501403-45501422 None:intergenic 35.0%
CTCCAATGAGATTCATTAAG+AGG + chr5.3:45502191-45502210 None:intergenic 35.0%
CTCCTCTTAATGAATCTCAT+TGG - chr5.3:45502186-45502205 MS.gene96654:CDS 35.0%
GCGTATTATTATCTCCTTTC+TGG + chr5.3:45503103-45503122 None:intergenic 35.0%
GTACGGATAAGAAGGATATT+TGG + chr5.3:45502970-45502989 None:intergenic 35.0%
GTATGCATATGCAAAGACAT+TGG - chr5.3:45502547-45502566 MS.gene96654:intron 35.0%
TCAAATCATCCAAATGCTTC+AGG - chr5.3:45502944-45502963 MS.gene96654:CDS 35.0%
TTATAGCGTTTGCAGTATTC+TGG + chr5.3:45502218-45502237 None:intergenic 35.0%
TTGATTTCGTACGGATAAGA+AGG + chr5.3:45502978-45502997 None:intergenic 35.0%
TTTAAAAGCAGATCTGATGG+TGG - chr5.3:45501323-45501342 MS.gene96654:CDS 35.0%
! ATGATACTAAGCATCGTCAA+AGG - chr5.3:45502683-45502702 MS.gene96654:intron 35.0%
! ATTGAGATTAGGAGAGTACA+AGG - chr5.3:45501856-45501875 MS.gene96654:intron 35.0%
! ATTTGCAGATGATTTGGAGA+AGG - chr5.3:45501254-45501273 MS.gene96654:intron 35.0%
! TTCTGGGTTCCTTTTATACA+TGG + chr5.3:45502071-45502090 None:intergenic 35.0%
AAAGACATTGGGAGAAAGAG+AGG - chr5.3:45502559-45502578 MS.gene96654:intron 40.0%
ACAAATCCCACTGTTGTGTT+TGG + chr5.3:45502828-45502847 None:intergenic 40.0%
CAAATCCCACTGTTGTGTTT+GGG + chr5.3:45502827-45502846 None:intergenic 40.0%
CACCACAAAAATCACTCAAC+TGG - chr5.3:45503133-45503152 MS.gene96654:CDS 40.0%
CAGCAATCACGTCATCTATA+TGG + chr5.3:45502851-45502870 None:intergenic 40.0%
GATCACAATTCGTTCGCAAT+AGG + chr5.3:45501829-45501848 None:intergenic 40.0%
TTTCACGTTTGCTTTGATGC+AGG + chr5.3:45502105-45502124 None:intergenic 40.0%
! AGATTAGGAGAGTACAAGGA+TGG - chr5.3:45501860-45501879 MS.gene96654:intron 40.0%
! GATATTTGGCCTGAAGCATT+TGG + chr5.3:45502956-45502975 None:intergenic 40.0%
! TCCCAGTTGAGTGATTTTTG+TGG + chr5.3:45503138-45503157 None:intergenic 40.0%
! TTGGATGATTTGAGACCAGT+GGG + chr5.3:45502937-45502956 None:intergenic 40.0%
! TTTGGATGATTTGAGACCAG+TGG + chr5.3:45502938-45502957 None:intergenic 40.0%
AAGTGGACCAACAACGAAAG+AGG + chr5.3:45502633-45502652 None:intergenic 45.0%
AATTCGTTCGCAATAGGCTC+GGG + chr5.3:45501823-45501842 None:intergenic 45.0%
ACTCCTCCATAGCCAATAAG+TGG + chr5.3:45502875-45502894 None:intergenic 45.0%
AGAACTACAGTGAGAAACCC+AGG - chr5.3:45501136-45501155 MS.gene96654:CDS 45.0%
AGTGGACCAACAACGAAAGA+GGG + chr5.3:45502632-45502651 None:intergenic 45.0%
ATCATGAGCAATGTGCTAGC+TGG + chr5.3:45502668-45502687 None:intergenic 45.0%
CAAAGCAAACGTGAAACGTG+TGG - chr5.3:45502109-45502128 MS.gene96654:CDS 45.0%
CAATTCGTTCGCAATAGGCT+CGG + chr5.3:45501824-45501843 None:intergenic 45.0%
CTCCTCCATAGCCAATAAGT+GGG + chr5.3:45502874-45502893 None:intergenic 45.0%
GAGTACCCAAACACAACAGT+GGG - chr5.3:45502819-45502838 MS.gene96654:intron 45.0%
GTTAACCCCTCTTTCGTTGT+TGG - chr5.3:45502623-45502642 MS.gene96654:intron 45.0%
TATAGGTGTAGCAGCCAGAA+AGG - chr5.3:45503086-45503105 MS.gene96654:intron 45.0%
! AGAGTACCCAAACACAACAG+TGG - chr5.3:45502818-45502837 MS.gene96654:intron 45.0%
! GCAGATGATTTGGAGAAGGT+TGG - chr5.3:45501258-45501277 MS.gene96654:intron 45.0%
! TAGGAGAGTACAAGGATGGT+TGG - chr5.3:45501864-45501883 MS.gene96654:intron 45.0%
! TTTTGATGAGGCAGTGACTG+GGG - chr5.3:45501353-45501372 MS.gene96654:CDS 45.0%
!! AAACTTGTTGCACATCGTCG+CGG + chr5.3:45502166-45502185 None:intergenic 45.0%
!! AAAGCAGATCTGATGGTGGA+AGG - chr5.3:45501327-45501346 MS.gene96654:CDS 45.0%
!! AAGCAGATCTGATGGTGGAA+GGG - chr5.3:45501328-45501347 MS.gene96654:CDS 45.0%
!! AGTTTTGATGAGGCAGTGAC+TGG - chr5.3:45501351-45501370 MS.gene96654:CDS 45.0%
!! GTTTTGATGAGGCAGTGACT+GGG - chr5.3:45501352-45501371 MS.gene96654:CDS 45.0%
CACATCGTCGCGGTATCTTA+TGG + chr5.3:45502156-45502175 None:intergenic 50.0%
CATTGGGAGAAAGAGAGGCT+TGG - chr5.3:45502564-45502583 MS.gene96654:intron 50.0%
CGTGATTGCTGCCCACTTAT+TGG - chr5.3:45502860-45502879 MS.gene96654:CDS 50.0%
GCAGGAGTTCAGCACATTCT+GGG + chr5.3:45502087-45502106 None:intergenic 50.0%
GGAGGATTGCAGAGGAAAGT+GGG - chr5.3:45502585-45502604 MS.gene96654:intron 50.0%
GTGGACCAACAACGAAAGAG+GGG + chr5.3:45502631-45502650 None:intergenic 50.0%
TACAAGCCTGCCAGATGCTT+TGG + chr5.3:45502898-45502917 None:intergenic 50.0%
TGCAGGAGTTCAGCACATTC+TGG + chr5.3:45502088-45502107 None:intergenic 50.0%
TGGAGGATTGCAGAGGAAAG+TGG - chr5.3:45502584-45502603 MS.gene96654:intron 50.0%
TGTGTTTGGGTACTCTCCTC+TGG + chr5.3:45502814-45502833 None:intergenic 50.0%
! ATGCCTGAGTTTTGCGTGAC+AGG - chr5.3:45501052-45501071 MS.gene96654:CDS 50.0%
! ATGGAGGAGTCCAAAGCATC+TGG - chr5.3:45502885-45502904 MS.gene96654:CDS 50.0%
! GGAAAGTGGGCTTGATCTAG+TGG - chr5.3:45502598-45502617 MS.gene96654:intron 50.0%
! TGCCCACTTATTGGCTATGG+AGG - chr5.3:45502869-45502888 MS.gene96654:CDS 50.0%
! TGCTGCCCACTTATTGGCTA+TGG - chr5.3:45502866-45502885 MS.gene96654:CDS 50.0%
!! GGTGGAAGGGAGTTTTGATG+AGG - chr5.3:45501341-45501360 MS.gene96654:CDS 50.0%
AGAGGCTTGGAGGATTGCAG+AGG - chr5.3:45502577-45502596 MS.gene96654:intron 55.0%
AGCAATGTGCTAGCTGGCTG+TGG + chr5.3:45502662-45502681 None:intergenic 55.0%
CCTCCTGTCACGCAAAACTC+AGG + chr5.3:45501058-45501077 None:intergenic 55.0%
GTTCAACCACAGTTGCCCAC+TGG - chr5.3:45502919-45502938 MS.gene96654:CDS 55.0%
TGGGAGAAAGAGAGGCTTGG+AGG - chr5.3:45502567-45502586 MS.gene96654:intron 55.0%
TTGAGACCAGTGGGCAACTG+TGG + chr5.3:45502928-45502947 None:intergenic 55.0%
! AGGAGTCCAAAGCATCTGGC+AGG - chr5.3:45502889-45502908 MS.gene96654:CDS 55.0%
! CCTGAGTTTTGCGTGACAGG+AGG - chr5.3:45501055-45501074 MS.gene96654:CDS 55.0%
!! TTTGCGTGACAGGAGGCACT+GGG - chr5.3:45501062-45501081 MS.gene96654:CDS 55.0%
!! TTTTGCGTGACAGGAGGCAC+TGG - chr5.3:45501061-45501080 MS.gene96654:CDS 55.0%
GAGGCAGTGACTGGGGTTGA+TGG - chr5.3:45501360-45501379 MS.gene96654:CDS 60.0%
GCTGGCTGTGGTGCAAGAAG+TGG + chr5.3:45502650-45502669 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 45501052 45503232 45501052 ID=MS.gene96654
chr5.3 mRNA 45501052 45503232 45501052 ID=MS.gene96654.t1;Parent=MS.gene96654
chr5.3 exon 45503127 45503232 45503127 ID=MS.gene96654.t1.exon1;Parent=MS.gene96654.t1
chr5.3 CDS 45503127 45503232 45503127 ID=cds.MS.gene96654.t1;Parent=MS.gene96654.t1
chr5.3 exon 45502850 45503022 45502850 ID=MS.gene96654.t1.exon2;Parent=MS.gene96654.t1
chr5.3 CDS 45502850 45503022 45502850 ID=cds.MS.gene96654.t1;Parent=MS.gene96654.t1
chr5.3 exon 45502046 45502231 45502046 ID=MS.gene96654.t1.exon3;Parent=MS.gene96654.t1
chr5.3 CDS 45502046 45502231 45502046 ID=cds.MS.gene96654.t1;Parent=MS.gene96654.t1
chr5.3 exon 45501580 45501742 45501580 ID=MS.gene96654.t1.exon4;Parent=MS.gene96654.t1
chr5.3 CDS 45501580 45501742 45501580 ID=cds.MS.gene96654.t1;Parent=MS.gene96654.t1
chr5.3 exon 45501287 45501476 45501287 ID=MS.gene96654.t1.exon5;Parent=MS.gene96654.t1
chr5.3 CDS 45501287 45501476 45501287 ID=cds.MS.gene96654.t1;Parent=MS.gene96654.t1
chr5.3 exon 45501052 45501193 45501052 ID=MS.gene96654.t1.exon6;Parent=MS.gene96654.t1
chr5.3 CDS 45501052 45501193 45501052 ID=cds.MS.gene96654.t1;Parent=MS.gene96654.t1
Gene Sequence

>MS.gene96654

ATGCCTGAGTTTTGCGTGACAGGAGGCACTGGGTTCATAGCAGCTTACCTAGTTAAAGCCTTATTAGAAAAAGGTCACATAGTGAGAACTACAGTGAGAAACCCAGGTATTAATATTTACTGATATATACATCCTTTTAATTTGATTGGTACATTAATTTTTCATTTTTAATCCACTTGTTTTTTCGCAAAATAAAAAATTAATTTGCAGATGATTTGGAGAAGGTTGGTTATCTAACTCAACTAAGTAGAGACAAAGAAAGATTAAAGATTTTAAAAGCAGATCTGATGGTGGAAGGGAGTTTTGATGAGGCAGTGACTGGGGTTGATGGTGTGTTTCATACAGCATCCCCTGTGATTGTTCCATATGATAACAACATTCAGGTACACTTTTCTATTTCTTATAAAAGTGTGTAATTAATTTTGAATTGTAGTTTTAATTTGAGAAATGATATTTATACAATTATTTTGGGATAACTTTTGTGACAATTTTCTGTCTCTATTTGTTTGATTTCCGTATCAATACTTAGTTTTTATGTAAATTTATTGTTGTCACAAAAGTTGTCGCACAAATTATTGTTCAAATAACACACCACTTTTAATTTTGCTTTCAAAATTGTAGTATCTTGTTCAGCTCACTTTTCATGAATACAGAAACATAAATCACTTTACATATACCTTACACATATTCAAAAAAGAACATTGTTGCTGAATTCATTATTCAAAGATTTAAAGCAATGATAATTTAATAATCTAACAAGGAAATTATCCCGAGCCTATTGCGAACGAATTGTGATCGTTAGATATTGAGATTAGGAGAGTACAAGGATGGTTGGAAGTAAAAATGATGAGCTGCTTGCGTATCGTGAACTTTTTCGTGAATTATTTTTCGTATCAAGTAGCATTTCTCATTGTCAAAAGAGAGTTTATTGAGTCATTTCAATAACATAGCATCATCAATATAACATATGGTAATTTGTTGCTATTTGAAATATGTTGTAGGCTACTTTGATTGATCCATGTATAAAAGGAACCCAGAATGTGCTGAACTCCTGCATCAAAGCAAACGTGAAACGTGTGGTGTTAACATCTTCTTGCTCTTCCATAAGATACCGCGACGATGTGCAACAAGTTTCTCCTCTTAATGAATCTCATTGGAGTGATCCAGAATACTGCAAACGCTATAATGTAACAACACTTCCTTCTCAATTATAAAGCCTTAAGTCATATCAAATTCTTGTTTGGATCATCACTTTGAGTAATATAGTTTACAAGAACTACATATATCATCACTTTAAGTAATGTCACATTCTTGTTTGGATCATCACTTTGAATTTTATGTTTTCTTTTTCAATATTTTTGAATCATTTTGAAACTAACCAAATTTTAAAATAATACTCAAACTAAAAATAGTCTGTTACATTAGTCTTTTTGGATCAATCAATTTAAATTAGCGTAATTTAAATTCAATGAAAATTGTGAGTTATGCAGCTTTGGTATGCATATGCAAAGACATTGGGAGAAAGAGAGGCTTGGAGGATTGCAGAGGAAAGTGGGCTTGATCTAGTGGTTGTTAACCCCTCTTTCGTTGTTGGTCCACTTCTTGCACCACAGCCAGCTAGCACATTGCTCATGATACTAAGCATCGTCAAAGGTTATATAATTCCCTCTCAAATGTGTTGTGATGTTAAGAATTCTTTGTATTCTATACCATATTTCTTATGACATTAAGTGTTATTTAATCTAAAAAATAACAGGTTCCAGAGGAGAGTACCCAAACACAACAGTGGGATTTGTCCATATAGATGACGTGATTGCTGCCCACTTATTGGCTATGGAGGAGTCCAAAGCATCTGGCAGGCTTGTATGTTCAACCACAGTTGCCCACTGGTCTCAAATCATCCAAATGCTTCAGGCCAAATATCCTTCTTATCCGTACGAAATCAAGTAAGTTTAAGAATTGCTGTATATCATATACTATATCAACCTTAATTTTGATATTATATAGAAAAAGTATTTATACTTTCTTTTCTACTATAGGTGTAGCAGCCAGAAAGGAGATAATAATACGCATAGCATGGACACCACAAAAATCACTCAACTGGGATTTCCTCAATTCAAAACTCTTGAACAAATGTTTGATGACTGCATCAAAAGCTTTCAAGACAAAGGCTTCCTATGA
Protein sequence

>MS.gene96654.t1

MPEFCVTGGTGFIAAYLVKALLEKGHIVRTTVRNPDDLEKVGYLTQLSRDKERLKILKADLMVEGSFDEAVTGVDGVFHTASPVIVPYDNNIQATLIDPCIKGTQNVLNSCIKANVKRVVLTSSCSSIRYRDDVQQVSPLNESHWSDPEYCKRYNLWYAYAKTLGEREAWRIAEESGLDLVVVNPSFVVGPLLAPQPASTLLMILSIVKGSRGEYPNTTVGFVHIDDVIAAHLLAMEESKASGRLVCSTTVAHWSQIIQMLQAKYPSYPYEIKCSSQKGDNNTHSMDTTKITQLGFPQFKTLEQMFDDCIKSFQDKGFL