Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026783.t1 | XP_024638013.1 | 96.2 | 106 | 4 | 0 | 1 | 106 | 1 | 106 | 1.20E-48 | 202.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026783.t1 | Q9SSC1 | 42.3 | 142 | 42 | 6 | 1 | 106 | 1 | 138 | 5.1e-16 | 85.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026783.t1 | A0A396HYZ6 | 96.2 | 106 | 4 | 0 | 1 | 106 | 1 | 106 | 8.6e-49 | 202.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene36231 | MS.gene026783 | PPI |
| MS.gene36229 | MS.gene026783 | PPI |
| MS.gene026783 | MS.gene86369 | PPI |
| MS.gene060879 | MS.gene026783 | PPI |
| MS.gene026783 | MS.gene89049 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026783.t1 | MTR_4g011060 | 96.190 | 105 | 4 | 0 | 1 | 105 | 1 | 105 | 1.08e-69 | 204 |
| MS.gene026783.t1 | MTR_2g103380 | 57.627 | 118 | 38 | 1 | 1 | 106 | 1 | 118 | 1.40e-39 | 128 |
| MS.gene026783.t1 | MTR_4g114050 | 53.191 | 94 | 32 | 4 | 1 | 93 | 1 | 83 | 5.51e-23 | 85.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene026783.t1 | AT1G80180 | 42.254 | 142 | 42 | 6 | 1 | 106 | 1 | 138 | 3.59e-24 | 89.7 |
| MS.gene026783.t1 | AT5G20100 | 47.959 | 98 | 47 | 2 | 3 | 96 | 2 | 99 | 6.60e-22 | 83.2 |
| MS.gene026783.t1 | AT1G15400 | 40.152 | 132 | 45 | 6 | 1 | 103 | 1 | 127 | 5.23e-20 | 79.3 |
| MS.gene026783.t1 | AT1G15400 | 40.152 | 132 | 45 | 6 | 1 | 103 | 1 | 127 | 5.47e-20 | 79.0 |
| MS.gene026783.t1 | AT1G15400 | 40.152 | 132 | 45 | 6 | 1 | 103 | 1 | 127 | 5.73e-20 | 79.3 |
Find 31 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGGTTCATCTGGTCTTGTT+TGG | 0.224185 | 4.1:+85091178 | MS.gene026783:CDS |
| AGGTTCATCTGGTCTTGTTT+GGG | 0.315565 | 4.1:+85091179 | MS.gene026783:CDS |
| TGTGGATTCTGTAGCCCTTT+TGG | 0.323362 | 4.1:+85091390 | MS.gene026783:CDS |
| CTACCTCGACCGACGTTTAC+CGG | 0.339840 | 4.1:-85091436 | None:intergenic |
| TGGGATGACAGATTCTTATC+AGG | 0.348639 | 4.1:+85091198 | MS.gene026783:CDS |
| CTGAATGATACTTCAGATCT+TGG | 0.351403 | 4.1:-85091150 | None:intergenic |
| GGAGACGGTGGTTCGATCGC+TGG | 0.391781 | 4.1:-85091354 | None:intergenic |
| TTCAGAAGATCAGGTTCATC+TGG | 0.432719 | 4.1:+85091168 | MS.gene026783:CDS |
| GAAGTATCATTCAGAAGATC+AGG | 0.445781 | 4.1:+85091159 | MS.gene026783:CDS |
| AGAAGCCATTCCACCGGTGC+TGG | 0.447002 | 4.1:+85091303 | MS.gene026783:CDS |
| GGTGGAATGGCTTCTTTGTA+CGG | 0.453050 | 4.1:-85091295 | None:intergenic |
| GAGACGGTGGTTCGATCGCT+GGG | 0.462717 | 4.1:-85091353 | None:intergenic |
| CTCGTCCAGCACCGGTGGAA+TGG | 0.498060 | 4.1:-85091308 | None:intergenic |
| TCTCCGAAGGTTTCTGCGTG+TGG | 0.512115 | 4.1:+85091372 | MS.gene026783:CDS |
| CATTCCACCGGTGCTGGACG+AGG | 0.514080 | 4.1:+85091309 | MS.gene026783:CDS |
| TACCTCGACCGACGTTTACC+GGG | 0.526089 | 4.1:-85091435 | None:intergenic |
| AATCCACACGCAGAAACCTT+CGG | 0.536395 | 4.1:-85091375 | None:intergenic |
| ACGGTATCCTCGTCCAGCAC+CGG | 0.568784 | 4.1:-85091316 | None:intergenic |
| AGCCCGGTAAACGTCGGTCG+AGG | 0.570758 | 4.1:+85091433 | MS.gene026783:CDS |
| TAGAGAGAGAACATCATGGC+TGG | 0.573751 | 4.1:+85091123 | None:intergenic |
| CACGCAGAAACCTTCGGAGA+CGG | 0.583247 | 4.1:-85091369 | None:intergenic |
| AAGGGTCAACGATCAAAGCC+CGG | 0.598386 | 4.1:+85091417 | MS.gene026783:CDS |
| GATCGAACCACCGTCTCCGA+AGG | 0.606120 | 4.1:+85091359 | MS.gene026783:CDS |
| CGGTAAACGTCGGTCGAGGT+AGG | 0.609038 | 4.1:+85091437 | MS.gene026783:CDS |
| GGAATGGCTTCTTTGTACGG+TGG | 0.613872 | 4.1:-85091292 | None:intergenic |
| GTATCCTCGTCCAGCACCGG+TGG | 0.623334 | 4.1:-85091313 | None:intergenic |
| GCTGGACGAGGATACCGTAC+CGG | 0.652103 | 4.1:+85091321 | MS.gene026783:CDS |
| GCAGAAACCTTCGGAGACGG+TGG | 0.660406 | 4.1:-85091366 | None:intergenic |
| AGACGGTGGTTCGATCGCTG+GGG | 0.689604 | 4.1:-85091352 | None:intergenic |
| GTACAAAGAAGCCATTCCAC+CGG | 0.692582 | 4.1:+85091297 | MS.gene026783:CDS |
| GATCAAAGCCCGGTAAACGT+CGG | 0.721418 | 4.1:+85091427 | MS.gene026783:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTGAATGATACTTCAGATCT+TGG | - | chr4.1:85091153-85091172 | None:intergenic | 35.0% | |
| ! | GAAGTATCATTCAGAAGATC+AGG | + | chr4.1:85091159-85091178 | MS.gene026783:CDS | 35.0% |
| !! | TGTAGCCCTTTTGGAAAAAA+GGG | + | chr4.1:85091399-85091418 | MS.gene026783:CDS | 35.0% |
| !!! | GTTGACCCTTTTTTCCAAAA+GGG | - | chr4.1:85091407-85091426 | None:intergenic | 35.0% |
| TGGGATGACAGATTCTTATC+AGG | + | chr4.1:85091198-85091217 | MS.gene026783:CDS | 40.0% | |
| TTCAGAAGATCAGGTTCATC+TGG | + | chr4.1:85091168-85091187 | MS.gene026783:CDS | 40.0% | |
| ! | GGTTTTCACATGAAGCTCTT+TGG | - | chr4.1:85091274-85091293 | None:intergenic | 40.0% |
| !! | AGGTTCATCTGGTCTTGTTT+GGG | + | chr4.1:85091179-85091198 | MS.gene026783:CDS | 40.0% |
| !! | CTGTAGCCCTTTTGGAAAAA+AGG | + | chr4.1:85091398-85091417 | MS.gene026783:CDS | 40.0% |
| !!! | CGTTGACCCTTTTTTCCAAA+AGG | - | chr4.1:85091408-85091427 | None:intergenic | 40.0% |
| AATCCACACGCAGAAACCTT+CGG | - | chr4.1:85091378-85091397 | None:intergenic | 45.0% | |
| GGTGGAATGGCTTCTTTGTA+CGG | - | chr4.1:85091298-85091317 | None:intergenic | 45.0% | |
| GTACAAAGAAGCCATTCCAC+CGG | + | chr4.1:85091297-85091316 | MS.gene026783:CDS | 45.0% | |
| ! | TGTGGATTCTGTAGCCCTTT+TGG | + | chr4.1:85091390-85091409 | MS.gene026783:CDS | 45.0% |
| !! | CAGGTTCATCTGGTCTTGTT+TGG | + | chr4.1:85091178-85091197 | MS.gene026783:CDS | 45.0% |
| AAGGGTCAACGATCAAAGCC+CGG | + | chr4.1:85091417-85091436 | MS.gene026783:CDS | 50.0% | |
| GATCAAAGCCCGGTAAACGT+CGG | + | chr4.1:85091427-85091446 | MS.gene026783:CDS | 50.0% | |
| GGAATGGCTTCTTTGTACGG+TGG | - | chr4.1:85091295-85091314 | None:intergenic | 50.0% | |
| CACGCAGAAACCTTCGGAGA+CGG | - | chr4.1:85091372-85091391 | None:intergenic | 55.0% | |
| CTACCTCGACCGACGTTTAC+CGG | - | chr4.1:85091439-85091458 | None:intergenic | 55.0% | |
| TACCTCGACCGACGTTTACC+GGG | - | chr4.1:85091438-85091457 | None:intergenic | 55.0% | |
| TCTCCGAAGGTTTCTGCGTG+TGG | + | chr4.1:85091372-85091391 | MS.gene026783:CDS | 55.0% | |
| ! | CTGGGGAGACTTTTCCGGTA+CGG | - | chr4.1:85091338-85091357 | None:intergenic | 55.0% |
| ! | GATCGCTGGGGAGACTTTTC+CGG | - | chr4.1:85091343-85091362 | None:intergenic | 55.0% |
| ACGGTATCCTCGTCCAGCAC+CGG | - | chr4.1:85091319-85091338 | None:intergenic | 60.0% | |
| AGAAGCCATTCCACCGGTGC+TGG | + | chr4.1:85091303-85091322 | MS.gene026783:CDS | 60.0% | |
| AGACGGTGGTTCGATCGCTG+GGG | - | chr4.1:85091355-85091374 | None:intergenic | 60.0% | |
| GAGACGGTGGTTCGATCGCT+GGG | - | chr4.1:85091356-85091375 | None:intergenic | 60.0% | |
| GATCGAACCACCGTCTCCGA+AGG | + | chr4.1:85091359-85091378 | MS.gene026783:CDS | 60.0% | |
| GCAGAAACCTTCGGAGACGG+TGG | - | chr4.1:85091369-85091388 | None:intergenic | 60.0% | |
| GCTGGACGAGGATACCGTAC+CGG | + | chr4.1:85091321-85091340 | MS.gene026783:CDS | 60.0% | |
| AGCCCGGTAAACGTCGGTCG+AGG | + | chr4.1:85091433-85091452 | MS.gene026783:CDS | 65.0% | |
| GGAGACGGTGGTTCGATCGC+TGG | - | chr4.1:85091357-85091376 | None:intergenic | 65.0% | |
| ! | CATTCCACCGGTGCTGGACG+AGG | + | chr4.1:85091309-85091328 | MS.gene026783:CDS | 65.0% |
| ! | GTATCCTCGTCCAGCACCGG+TGG | - | chr4.1:85091316-85091335 | None:intergenic | 65.0% |
| !! | CTCGTCCAGCACCGGTGGAA+TGG | - | chr4.1:85091311-85091330 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 85091138 | 85091458 | 85091138 | ID=MS.gene026783 |
| chr4.1 | mRNA | 85091138 | 85091458 | 85091138 | ID=MS.gene026783.t1;Parent=MS.gene026783 |
| chr4.1 | exon | 85091138 | 85091458 | 85091138 | ID=MS.gene026783.t1.exon1;Parent=MS.gene026783.t1 |
| chr4.1 | CDS | 85091138 | 85091458 | 85091138 | ID=cds.MS.gene026783.t1;Parent=MS.gene026783.t1 |
| Gene Sequence |
| Protein sequence |
