AlfalfaGEDB | MS.gene86369

M. sativa cultivar XinJiangDaYe / MS.gene86369

KEGG: -
GO: -
PFAM: PF15697.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86369.t1	XP_003597862.2	99.2	118	1	0	1	118	1	118	5.70E-52	213.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86369.t1	Q9SSC1	46.4	138	54	6	1	118	1	138	7.7e-21	101.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86369.t1	Q2HVW4	99.2	118	1	0	1	118	1	118	4.1e-52	213.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049378	MS.gene86369	0.820098	8.21E-53	-1.69E-46
MS.gene051935	MS.gene86369	0.819038	1.43E-52	-1.69E-46
MS.gene052108	MS.gene86369	0.809372	1.95E-50	-1.69E-46
MS.gene052176	MS.gene86369	0.832523	9.00E-56	-1.69E-46
MS.gene052333	MS.gene86369	0.830357	3.07E-55	-1.69E-46
MS.gene052389	MS.gene86369	0.821668	3.57E-53	-1.69E-46
MS.gene052586	MS.gene86369	0.839997	1.13E-57	-1.69E-46
MS.gene05415	MS.gene86369	0.80726	5.51E-50	-1.69E-46
MS.gene054468	MS.gene86369	0.818523	1.88E-52	-1.69E-46
MS.gene057026	MS.gene86369	0.818462	1.94E-52	-1.69E-46
MS.gene058498	MS.gene86369	0.818178	2.24E-52	-1.69E-46
MS.gene058500	MS.gene86369	0.814167	1.77E-51	-1.69E-46
MS.gene058502	MS.gene86369	0.8031	4.09E-49	-1.69E-46
MS.gene058897	MS.gene86369	0.842668	2.25E-58	-1.69E-46
MS.gene060572	MS.gene86369	0.81098	8.80E-51	-1.69E-46
MS.gene060879	MS.gene86369	0.842938	1.91E-58	-1.69E-46
MS.gene061040	MS.gene86369	-0.811926	5.48E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene75382	MS.gene86369	PPI
MS.gene034054	MS.gene86369	PPI
MS.gene026783	MS.gene86369	PPI
MS.gene36231	MS.gene86369	PPI
MS.gene89049	MS.gene86369	PPI
MS.gene36229	MS.gene86369	PPI
MS.gene60230	MS.gene86369	PPI
MS.gene060879	MS.gene86369	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86369.t1	MTR_2g103380	99.153	118	1	0	1	118	1	118	3.74e-84	241
MS.gene86369.t1	MTR_4g011060	60.684	117	34	2	1	117	1	105	6.50e-41	132
MS.gene86369.t1	MTR_4g114050	43.548	124	41	5	1	118	1	101	3.25e-23	86.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene86369.t1	AT1G80180	48.551	138	51	6	1	118	1	138	1.34e-30	106
MS.gene86369.t1	AT1G15400	49.180	122	44	5	1	105	1	121	9.27e-29	102
MS.gene86369.t1	AT1G15400	49.180	122	44	5	1	105	1	121	1.10e-28	101
MS.gene86369.t1	AT1G15400	49.180	122	44	5	1	105	1	121	1.54e-28	101
MS.gene86369.t1	AT5G20100	47.273	110	42	5	3	108	2	99	1.27e-18	75.5

Find 28 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGTTCATCAGGGTTTGTT+TGG	0.296640	2.3:-1557538	MS.gene86369:CDS
CTCCTAGGATTTCTGCTTGT+GGG	0.299207	2.3:-1557307	MS.gene86369:CDS
TTCAGAAGACAAGGTTCATC+AGG	0.369694	2.3:-1557548	MS.gene86369:CDS
GTTCTTGTTGATTCTGACTC+TGG	0.396706	2.3:+1557477	None:intergenic
AGGTTCATCAGGGTTTGTTT+GGG	0.404671	2.3:-1557537	MS.gene86369:CDS
CTTCTCTGAACAGTATTGAT+TGG	0.435131	2.3:+1557395	None:intergenic
CGTTCTAACGGTGGAGGACG+TGG	0.452280	2.3:-1557371	MS.gene86369:CDS
CTCTACCTGGATCGATGCTT+AGG	0.481698	2.3:+1557242	None:intergenic
TTGGGAAAGTAGGAGAGAAG+AGG	0.503813	2.3:-1557271	MS.gene86369:CDS
TCTCCTAGGATTTCTGCTTG+TGG	0.524460	2.3:-1557308	MS.gene86369:CDS
CGATTGAGCCACCGTCTCCT+AGG	0.529012	2.3:-1557322	MS.gene86369:CDS
AACCACCTAAGCATCGATCC+AGG	0.548665	2.3:-1557247	MS.gene86369:CDS
GGAGACGGTGGCTCAATCGC+CGG	0.558530	2.3:+1557326	None:intergenic
GATGCAACATCACCACAGCT+TGG	0.569916	2.3:-1557419	MS.gene86369:CDS
GGAGGACGTGGTTACCGTAC+CGG	0.573123	2.3:-1557359	MS.gene86369:CDS
CCGTACCGGAAAAGTATCTC+CGG	0.581778	2.3:-1557345	MS.gene86369:CDS
AACCCACAAGCAGAAATCCT+AGG	0.594122	2.3:+1557305	None:intergenic
TACCTGGATCGATGCTTAGG+TGG	0.595835	2.3:+1557245	None:intergenic
TCAGAAGACAAGGTTCATCA+GGG	0.600967	2.3:-1557547	MS.gene86369:CDS
AGAAGTCGTTCTAACGGTGG+AGG	0.607642	2.3:-1557377	MS.gene86369:CDS
AAAGAGAAACCAGACATGGA+AGG	0.620120	2.3:-1557593	None:intergenic
GTTCAGAGAAGTCGTTCTAA+CGG	0.637358	2.3:-1557383	MS.gene86369:CDS
CAAGCAGAAATCCTAGGAGA+CGG	0.637647	2.3:+1557311	None:intergenic
GCAGTATCATTCAGAAGACA+AGG	0.648817	2.3:-1557557	MS.gene86369:CDS
CAGAGAAGTCGTTCTAACGG+TGG	0.679356	2.3:-1557380	MS.gene86369:CDS
GCAGAAATCCTAGGAGACGG+TGG	0.696269	2.3:+1557314	None:intergenic
GCTGTGGTGATGTTGCATCA+CGG	0.699454	2.3:+1557423	None:intergenic
GTATTGATTGGTCCAAGCTG+TGG	0.700900	2.3:+1557407	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTTCTCTGAACAGTATTGAT+TGG	+	chr2.3:1557430-1557449	None:intergenic	35.0%
	GCAGTATCATTCAGAAGACA+AGG	-	chr2.3:1557265-1557284	MS.gene86369:CDS	40.0%
	GTTCAGAGAAGTCGTTCTAA+CGG	-	chr2.3:1557439-1557458	MS.gene86369:CDS	40.0%
	TCAGAAGACAAGGTTCATCA+GGG	-	chr2.3:1557275-1557294	MS.gene86369:CDS	40.0%
	TTCAGAAGACAAGGTTCATC+AGG	-	chr2.3:1557274-1557293	MS.gene86369:CDS	40.0%
!	AAGGTTCATCAGGGTTTGTT+TGG	-	chr2.3:1557284-1557303	MS.gene86369:CDS	40.0%
!	AGGTTCATCAGGGTTTGTTT+GGG	-	chr2.3:1557285-1557304	MS.gene86369:CDS	40.0%
!	ATTTCTGCTTGTGGGTTTTG+TGG	-	chr2.3:1557523-1557542	MS.gene86369:CDS	40.0%
!!	GTTCTTGTTGATTCTGACTC+TGG	+	chr2.3:1557348-1557367	None:intergenic	40.0%
	AACCCACAAGCAGAAATCCT+AGG	+	chr2.3:1557520-1557539	None:intergenic	45.0%
	CAAGCAGAAATCCTAGGAGA+CGG	+	chr2.3:1557514-1557533	None:intergenic	45.0%
	CTCCTAGGATTTCTGCTTGT+GGG	-	chr2.3:1557515-1557534	MS.gene86369:CDS	45.0%
	TCTCCTAGGATTTCTGCTTG+TGG	-	chr2.3:1557514-1557533	MS.gene86369:CDS	45.0%
	TTGGGAAAGTAGGAGAGAAG+AGG	-	chr2.3:1557551-1557570	MS.gene86369:CDS	45.0%
!	AATCGCCGGAGATACTTTTC+CGG	+	chr2.3:1557485-1557504	None:intergenic	45.0%
!!	GTATTGATTGGTCCAAGCTG+TGG	+	chr2.3:1557418-1557437	None:intergenic	45.0%
!!	GTGGGTTTTGTGGTGCTTTT+GGG	-	chr2.3:1557533-1557552	MS.gene86369:CDS	45.0%
!!	TGTGGGTTTTGTGGTGCTTT+TGG	-	chr2.3:1557532-1557551	MS.gene86369:CDS	45.0%
!!	TGTGGTGCTTTTGGGAAAGT+AGG	-	chr2.3:1557541-1557560	MS.gene86369:CDS	45.0%
	AGAAGTCGTTCTAACGGTGG+AGG	-	chr2.3:1557445-1557464	MS.gene86369:CDS	50.0%
	CAGAGAAGTCGTTCTAACGG+TGG	-	chr2.3:1557442-1557461	MS.gene86369:CDS	50.0%
	GATGCAACATCACCACAGCT+TGG	-	chr2.3:1557403-1557422	MS.gene86369:CDS	50.0%
	TACCTGGATCGATGCTTAGG+TGG	+	chr2.3:1557580-1557599	None:intergenic	50.0%
!	CCGGAGATACTTTTCCGGTA+CGG	+	chr2.3:1557480-1557499	None:intergenic	50.0%
!	GCTGTGGTGATGTTGCATCA+CGG	+	chr2.3:1557402-1557421	None:intergenic	50.0%
!!	AACCACCTAAGCATCGATCC+AGG	-	chr2.3:1557575-1557594	MS.gene86369:CDS	50.0%
!!	CCGTACCGGAAAAGTATCTC+CGG	-	chr2.3:1557477-1557496	MS.gene86369:CDS	50.0%
	GCAGAAATCCTAGGAGACGG+TGG	+	chr2.3:1557511-1557530	None:intergenic	55.0%
	CGATTGAGCCACCGTCTCCT+AGG	-	chr2.3:1557500-1557519	MS.gene86369:CDS	60.0%
	CGTTCTAACGGTGGAGGACG+TGG	-	chr2.3:1557451-1557470	MS.gene86369:CDS	60.0%
	GGAGGACGTGGTTACCGTAC+CGG	-	chr2.3:1557463-1557482	MS.gene86369:CDS	60.0%
	GGAGACGGTGGCTCAATCGC+CGG	+	chr2.3:1557499-1557518	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	1557244	1557600	1557244	ID=MS.gene86369
chr2.3	mRNA	1557244	1557600	1557244	ID=MS.gene86369.t1;Parent=MS.gene86369
chr2.3	exon	1557244	1557600	1557244	ID=MS.gene86369.t1.exon1;Parent=MS.gene86369.t1
chr2.3	CDS	1557244	1557600	1557244	ID=cds.MS.gene86369.t1;Parent=MS.gene86369.t1

Gene Sequence	>MS.gene86369 ATGGAAGGTTTACAAAGATCTGCAGTATCATTCAGAAGACAAGGTTCATCAGGGTTTGTTTGGGATGATAGATTTCTACAAGAAGAAATAAACAAGATGAACCAGAGTCAGAATCAACAAGAACAAGAAGACAAAGATGAAATCAAAGAGATGAACCGTGATGCAACATCACCACAGCTTGGACCAATCAATACTGTTCAGAGAAGTCGTTCTAACGGTGGAGGACGTGGTTACCGTACCGGAAAAGTATCTCCGGCGATTGAGCCACCGTCTCCTAGGATTTCTGCTTGTGGGTTTTGTGGTGCTTTTGGGAAAGTAGGAGAGAAGAGGAAACCACCTAAGCATCGATCCAGGTAG
Protein sequence	>MS.gene86369.t1 MEGLQRSAVSFRRQGSSGFVWDDRFLQEEINKMNQSQNQQEQEDKDEIKEMNRDATSPQLGPINTVQRSRSNGGGRGYRTGKVSPAIEPPSPRISACGFCGAFGKVGEKRKPPKHRSR