AlfalfaGEDB | MS.gene89049

M. sativa cultivar XinJiangDaYe / MS.gene89049

KEGG: -
GO: -
PFAM: PF15697.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89049.t1	XP_003597862.2	99.2	118	1	0	1	118	1	118	5.70E-52	213.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89049.t1	Q9SSC1	46.4	138	54	6	1	118	1	138	7.7e-21	101.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89049.t1	Q2HVW4	99.2	118	1	0	1	118	1	118	4.1e-52	213.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049230	MS.gene89049	0.835075	2.07E-56	-1.69E-46
MS.gene049293	MS.gene89049	-0.80249	5.47E-49	-1.69E-46
MS.gene049378	MS.gene89049	0.854244	1.40E-61	-1.69E-46
MS.gene049669	MS.gene89049	0.830392	3.01E-55	-1.69E-46
MS.gene050227	MS.gene89049	0.805982	1.03E-49	-1.69E-46
MS.gene050228	MS.gene89049	0.80013	1.67E-48	-1.69E-46
MS.gene050312	MS.gene89049	0.800019	1.75E-48	-1.69E-46
MS.gene050750	MS.gene89049	0.801524	8.65E-49	-1.69E-46
MS.gene050953	MS.gene89049	0.810986	8.77E-51	-1.69E-46
MS.gene051064	MS.gene89049	0.837298	5.65E-57	-1.69E-46
MS.gene051368	MS.gene89049	0.816456	5.48E-52	-1.69E-46
MS.gene052176	MS.gene89049	0.848025	7.97E-60	-1.69E-46
MS.gene052302	MS.gene89049	0.803848	2.87E-49	-1.69E-46
MS.gene052309	MS.gene89049	-0.818729	1.68E-52	-1.69E-46
MS.gene052376	MS.gene89049	0.812812	3.51E-51	-1.69E-46
MS.gene052389	MS.gene89049	0.838575	2.65E-57	-1.69E-46
MS.gene052586	MS.gene89049	0.857851	1.23E-62	-1.69E-46
MS.gene052685	MS.gene89049	0.806496	8.00E-50	-1.69E-46
MS.gene053298	MS.gene89049	0.800187	1.62E-48	-1.69E-46
MS.gene053340	MS.gene89049	0.831921	1.27E-55	-1.69E-46
MS.gene053963	MS.gene89049	0.818001	2.46E-52	-1.69E-46
MS.gene054222	MS.gene89049	0.814265	1.68E-51	-1.69E-46
MS.gene055701	MS.gene89049	0.824309	8.66E-54	-1.69E-46
MS.gene056064	MS.gene89049	0.806676	7.33E-50	-1.69E-46
MS.gene056068	MS.gene89049	0.8113	7.50E-51	-1.69E-46
MS.gene056506	MS.gene89049	0.813671	2.27E-51	-1.69E-46
MS.gene05659	MS.gene89049	0.807199	5.68E-50	-1.69E-46
MS.gene057026	MS.gene89049	0.835735	1.41E-56	-1.69E-46
MS.gene057079	MS.gene89049	0.803886	2.81E-49	-1.69E-46
MS.gene057156	MS.gene89049	-0.803544	3.31E-49	-1.69E-46
MS.gene058223	MS.gene89049	-0.800359	1.50E-48	-1.69E-46
MS.gene058498	MS.gene89049	0.833735	4.50E-56	-1.69E-46
MS.gene058500	MS.gene89049	0.820203	7.76E-53	-1.69E-46
MS.gene060572	MS.gene89049	0.808935	2.42E-50	-1.69E-46
MS.gene060586	MS.gene89049	0.817928	2.56E-52	-1.69E-46
MS.gene060879	MS.gene89049	0.963533	3.35E-122	-1.69E-46
MS.gene061040	MS.gene89049	-0.831143	1.97E-55	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene89049	MS.gene60230	PPI
MS.gene060879	MS.gene89049	PPI
MS.gene89049	MS.gene86369	PPI
MS.gene89049	MS.gene034054	PPI
MS.gene026783	MS.gene89049	PPI
MS.gene36231	MS.gene89049	PPI
MS.gene89049	MS.gene75382	PPI
MS.gene36229	MS.gene89049	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89049.t1	MTR_2g103380	99.153	118	1	0	1	118	1	118	3.74e-84	241
MS.gene89049.t1	MTR_4g011060	60.684	117	34	2	1	117	1	105	6.50e-41	132
MS.gene89049.t1	MTR_4g114050	43.548	124	41	5	1	118	1	101	3.25e-23	86.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene89049.t1	AT1G80180	48.551	138	51	6	1	118	1	138	1.34e-30	106
MS.gene89049.t1	AT1G15400	49.180	122	44	5	1	105	1	121	9.27e-29	102
MS.gene89049.t1	AT1G15400	49.180	122	44	5	1	105	1	121	1.10e-28	101
MS.gene89049.t1	AT1G15400	49.180	122	44	5	1	105	1	121	1.54e-28	101
MS.gene89049.t1	AT5G20100	47.273	110	42	5	3	108	2	99	1.27e-18	75.5

Find 29 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGTTCATCAGGGTTTGTT+TGG	0.296640	2.4:-2078316	MS.gene89049:CDS
TTCAGAAGACAAGGTTCATC+AGG	0.369694	2.4:-2078326	MS.gene89049:CDS
GTTCTTGTTGATTCTGACTC+TGG	0.396706	2.4:+2078255	None:intergenic
AGGTTCATCAGGGTTTGTTT+GGG	0.404671	2.4:-2078315	MS.gene89049:CDS
CTCCTAGGATTTCTGCCTGT+GGG	0.417892	2.4:-2078085	MS.gene89049:CDS
CTTCTCTGAACAGTATTGAT+TGG	0.435131	2.4:+2078173	None:intergenic
CGTTCTAACGGTGGAGGACG+TGG	0.452280	2.4:-2078149	MS.gene89049:CDS
AACTACCTTGATCGATGCTT+AGG	0.486228	2.4:+2078020	None:intergenic
TTGGGAAAGTAGGGGAGAAG+AGG	0.507939	2.4:-2078049	MS.gene89049:CDS
CGATTGAGCCACCGTCTCCT+AGG	0.529012	2.4:-2078100	MS.gene89049:CDS
AACCCACAGGCAGAAATCCT+AGG	0.546823	2.4:+2078083	None:intergenic
GGAGACGGTGGCTCAATCGC+CGG	0.558530	2.4:+2078104	None:intergenic
CCGTACCGGAAAAGTTTCTC+CGG	0.558933	2.4:-2078123	MS.gene89049:CDS
AAAAGCACCACAAAACCCAC+AGG	0.561933	2.4:+2078070	None:intergenic
GATGCAACATCACCACAGCT+TGG	0.569916	2.4:-2078197	MS.gene89049:CDS
GGAGGACGTGGTTACCGTAC+CGG	0.573123	2.4:-2078137	MS.gene89049:CDS
TCTCCTAGGATTTCTGCCTG+TGG	0.574828	2.4:-2078086	MS.gene89049:CDS
AACCACCTAAGCATCGATCA+AGG	0.579242	2.4:-2078025	MS.gene89049:CDS
TCAGAAGACAAGGTTCATCA+GGG	0.600967	2.4:-2078325	MS.gene89049:CDS
TACCTTGATCGATGCTTAGG+TGG	0.600976	2.4:+2078023	None:intergenic
AGAAGTCGTTCTAACGGTGG+AGG	0.607642	2.4:-2078155	MS.gene89049:CDS
ACAGAGAAACCAGACATGGA+AGG	0.634834	2.4:-2078371	None:intergenic
GTTCAGAGAAGTCGTTCTAA+CGG	0.637358	2.4:-2078161	MS.gene89049:CDS
CAGGCAGAAATCCTAGGAGA+CGG	0.637647	2.4:+2078089	None:intergenic
GCAGTATCATTCAGAAGACA+AGG	0.648817	2.4:-2078335	MS.gene89049:CDS
CAGAGAAGTCGTTCTAACGG+TGG	0.679356	2.4:-2078158	MS.gene89049:CDS
GCAGAAATCCTAGGAGACGG+TGG	0.696269	2.4:+2078092	None:intergenic
GCTGTGGTGATGTTGCATCA+CGG	0.699454	2.4:+2078201	None:intergenic
GTATTGATTGGTCCAAGCTG+TGG	0.700900	2.4:+2078185	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTTCTCTGAACAGTATTGAT+TGG	+	chr2.4:2078208-2078227	None:intergenic	35.0%
	GCAGTATCATTCAGAAGACA+AGG	-	chr2.4:2078043-2078062	MS.gene89049:CDS	40.0%
	GTTCAGAGAAGTCGTTCTAA+CGG	-	chr2.4:2078217-2078236	MS.gene89049:CDS	40.0%
	TCAGAAGACAAGGTTCATCA+GGG	-	chr2.4:2078053-2078072	MS.gene89049:CDS	40.0%
	TTCAGAAGACAAGGTTCATC+AGG	-	chr2.4:2078052-2078071	MS.gene89049:CDS	40.0%
!	AAGGTTCATCAGGGTTTGTT+TGG	-	chr2.4:2078062-2078081	MS.gene89049:CDS	40.0%
!	AGGTTCATCAGGGTTTGTTT+GGG	-	chr2.4:2078063-2078082	MS.gene89049:CDS	40.0%
!!	GTTCTTGTTGATTCTGACTC+TGG	+	chr2.4:2078126-2078145	None:intergenic	40.0%
	TACCTTGATCGATGCTTAGG+TGG	+	chr2.4:2078358-2078377	None:intergenic	45.0%
!	AATCGCCGGAGAAACTTTTC+CGG	+	chr2.4:2078263-2078282	None:intergenic	45.0%
!	ATTTCTGCCTGTGGGTTTTG+TGG	-	chr2.4:2078301-2078320	MS.gene89049:CDS	45.0%
!!	AAAAGCACCACAAAACCCAC+AGG	+	chr2.4:2078311-2078330	None:intergenic	45.0%
!!	AACCACCTAAGCATCGATCA+AGG	-	chr2.4:2078353-2078372	MS.gene89049:CDS	45.0%
!!	GTATTGATTGGTCCAAGCTG+TGG	+	chr2.4:2078196-2078215	None:intergenic	45.0%
!!	GTGGGTTTTGTGGTGCTTTT+GGG	-	chr2.4:2078311-2078330	MS.gene89049:CDS	45.0%
!!	GTGGTGCTTTTGGGAAAGTA+GGG	-	chr2.4:2078320-2078339	MS.gene89049:CDS	45.0%
!!	TGGTGCTTTTGGGAAAGTAG+GGG	-	chr2.4:2078321-2078340	MS.gene89049:CDS	45.0%
!!	TGTGGGTTTTGTGGTGCTTT+TGG	-	chr2.4:2078310-2078329	MS.gene89049:CDS	45.0%
!!	TGTGGTGCTTTTGGGAAAGT+AGG	-	chr2.4:2078319-2078338	MS.gene89049:CDS	45.0%
	AACCCACAGGCAGAAATCCT+AGG	+	chr2.4:2078298-2078317	None:intergenic	50.0%
	AGAAGTCGTTCTAACGGTGG+AGG	-	chr2.4:2078223-2078242	MS.gene89049:CDS	50.0%
	CAGAGAAGTCGTTCTAACGG+TGG	-	chr2.4:2078220-2078239	MS.gene89049:CDS	50.0%
	CAGGCAGAAATCCTAGGAGA+CGG	+	chr2.4:2078292-2078311	None:intergenic	50.0%
	CCGTACCGGAAAAGTTTCTC+CGG	-	chr2.4:2078255-2078274	MS.gene89049:CDS	50.0%
	CTCCTAGGATTTCTGCCTGT+GGG	-	chr2.4:2078293-2078312	MS.gene89049:CDS	50.0%
	GATGCAACATCACCACAGCT+TGG	-	chr2.4:2078181-2078200	MS.gene89049:CDS	50.0%
	TCTCCTAGGATTTCTGCCTG+TGG	-	chr2.4:2078292-2078311	MS.gene89049:CDS	50.0%
	TTGGGAAAGTAGGGGAGAAG+AGG	-	chr2.4:2078329-2078348	MS.gene89049:CDS	50.0%
!	CCGGAGAAACTTTTCCGGTA+CGG	+	chr2.4:2078258-2078277	None:intergenic	50.0%
!	GCTGTGGTGATGTTGCATCA+CGG	+	chr2.4:2078180-2078199	None:intergenic	50.0%
	GCAGAAATCCTAGGAGACGG+TGG	+	chr2.4:2078289-2078308	None:intergenic	55.0%
	CGATTGAGCCACCGTCTCCT+AGG	-	chr2.4:2078278-2078297	MS.gene89049:CDS	60.0%
	CGTTCTAACGGTGGAGGACG+TGG	-	chr2.4:2078229-2078248	MS.gene89049:CDS	60.0%
	GGAGGACGTGGTTACCGTAC+CGG	-	chr2.4:2078241-2078260	MS.gene89049:CDS	60.0%
	GGAGACGGTGGCTCAATCGC+CGG	+	chr2.4:2078277-2078296	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	2078022	2078378	2078022	ID=MS.gene89049
chr2.4	mRNA	2078022	2078378	2078022	ID=MS.gene89049.t1;Parent=MS.gene89049
chr2.4	exon	2078022	2078378	2078022	ID=MS.gene89049.t1.exon1;Parent=MS.gene89049.t1
chr2.4	CDS	2078022	2078378	2078022	ID=cds.MS.gene89049.t1;Parent=MS.gene89049.t1

Gene Sequence	>MS.gene89049 ATGGAAGGTTTACAAAGATCTGCAGTATCATTCAGAAGACAAGGTTCATCAGGGTTTGTTTGGGATGATAGATTTCTACAAGAAGAAATAAACAAGATGAACCAGAGTCAGAATCAACAAGAACAAGAAGACAAAGATGAAATCAAAGAGATGAACCGTGATGCAACATCACCACAGCTTGGACCAATCAATACTGTTCAGAGAAGTCGTTCTAACGGTGGAGGACGTGGTTACCGTACCGGAAAAGTTTCTCCGGCGATTGAGCCACCGTCTCCTAGGATTTCTGCCTGTGGGTTTTGTGGTGCTTTTGGGAAAGTAGGGGAGAAGAGGAAACCACCTAAGCATCGATCAAGGTAG
Protein sequence	>MS.gene89049.t1 MEGLQRSAVSFRRQGSSGFVWDDRFLQEEINKMNQSQNQQEQEDKDEIKEMNRDATSPQLGPINTVQRSRSNGGGRGYRTGKVSPAIEPPSPRISACGFCGAFGKVGEKRKPPKHRSR