AlfalfaGEDB | MS.gene36231

M. sativa cultivar XinJiangDaYe / MS.gene36231

KEGG: -
GO: -
PFAM: PF15697.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36231.t1	XP_003597862.2	99.2	118	1	0	1	118	1	118	5.70E-52	213.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36231.t1	Q9SSC1	46.4	138	54	6	1	118	1	138	7.7e-21	101.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36231.t1	Q2HVW4	99.2	118	1	0	1	118	1	118	4.1e-52	213.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049230	MS.gene36231	0.82803	1.13E-54	-1.69E-46
MS.gene049254	MS.gene36231	0.800045	1.73E-48	-1.69E-46
MS.gene049293	MS.gene36231	-0.801131	1.04E-48	-1.69E-46
MS.gene049378	MS.gene36231	0.851776	7.12E-61	-1.69E-46
MS.gene049419	MS.gene36231	-0.807948	3.94E-50	-1.69E-46
MS.gene049669	MS.gene36231	0.85333	2.57E-61	-1.69E-46
MS.gene050105	MS.gene36231	0.806896	6.58E-50	-1.69E-46
MS.gene050227	MS.gene36231	0.80257	5.27E-49	-1.69E-46
MS.gene050228	MS.gene36231	0.800982	1.12E-48	-1.69E-46
MS.gene050312	MS.gene36231	0.810156	1.33E-50	-1.69E-46
MS.gene050355	MS.gene36231	0.813909	2.02E-51	-1.69E-46
MS.gene050750	MS.gene36231	0.82211	2.82E-53	-1.69E-46
MS.gene050953	MS.gene36231	0.827743	1.32E-54	-1.69E-46
MS.gene051064	MS.gene36231	0.837553	4.86E-57	-1.69E-46
MS.gene051368	MS.gene36231	0.840462	8.57E-58	-1.69E-46
MS.gene051926	MS.gene36231	-0.800264	1.56E-48	-1.69E-46
MS.gene052176	MS.gene36231	0.849388	3.34E-60	-1.69E-46
MS.gene052309	MS.gene36231	-0.805629	1.22E-49	-1.69E-46
MS.gene052333	MS.gene36231	0.818369	2.03E-52	-1.69E-46
MS.gene052376	MS.gene36231	0.8066	7.60E-50	-1.69E-46
MS.gene052389	MS.gene36231	0.852543	4.31E-61	-1.69E-46
MS.gene052586	MS.gene36231	0.874643	5.75E-68	-1.69E-46
MS.gene052685	MS.gene36231	0.818091	2.35E-52	-1.69E-46
MS.gene052954	MS.gene36231	0.806528	7.87E-50	-1.69E-46
MS.gene053006	MS.gene36231	0.814079	1.85E-51	-1.69E-46
MS.gene053340	MS.gene36231	0.813177	2.92E-51	-1.69E-46
MS.gene05365	MS.gene36231	0.803719	3.05E-49	-1.69E-46
MS.gene053963	MS.gene36231	0.823548	1.31E-53	-1.69E-46
MS.gene054222	MS.gene36231	0.824161	9.38E-54	-1.69E-46
MS.gene054291	MS.gene36231	-0.822228	2.65E-53	-1.69E-46
MS.gene054405	MS.gene36231	0.817713	2.86E-52	-1.69E-46
MS.gene054468	MS.gene36231	0.819943	8.91E-53	-1.69E-46
MS.gene054576	MS.gene36231	0.8013	9.61E-49	-1.69E-46
MS.gene055225	MS.gene36231	0.81701	4.12E-52	-1.69E-46
MS.gene055701	MS.gene36231	0.84135	5.01E-58	-1.69E-46
MS.gene056064	MS.gene36231	0.81083	9.49E-51	-1.69E-46
MS.gene056068	MS.gene36231	0.804659	1.94E-49	-1.69E-46
MS.gene056506	MS.gene36231	0.81447	1.52E-51	-1.69E-46
MS.gene057026	MS.gene36231	0.844856	5.83E-59	-1.69E-46
MS.gene057156	MS.gene36231	-0.832525	8.99E-56	-1.69E-46
MS.gene057194	MS.gene36231	0.801934	7.12E-49	-1.69E-46
MS.gene058498	MS.gene36231	0.844206	8.73E-59	-1.69E-46
MS.gene058500	MS.gene36231	0.831686	1.45E-55	-1.69E-46
MS.gene058502	MS.gene36231	0.817293	3.55E-52	-1.69E-46
MS.gene05954	MS.gene36231	0.804682	1.92E-49	-1.69E-46
MS.gene059952	MS.gene36231	0.800642	1.31E-48	-1.69E-46
MS.gene060012	MS.gene36231	0.800524	1.38E-48	-1.69E-46
MS.gene060322	MS.gene36231	0.803139	4.02E-49	-1.69E-46
MS.gene060572	MS.gene36231	0.806153	9.44E-50	-1.69E-46
MS.gene060879	MS.gene36231	0.990729	4.90E-184	-1.69E-46
MS.gene061040	MS.gene36231	-0.846272	2.41E-59	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene36229	MS.gene36231	PPI
MS.gene36231	MS.gene026783	PPI
MS.gene36231	MS.gene60230	PPI
MS.gene36231	MS.gene034054	PPI
MS.gene36231	MS.gene86369	PPI
MS.gene060879	MS.gene36231	PPI
MS.gene36231	MS.gene75382	PPI
MS.gene36231	MS.gene89049	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36231.t1	MTR_2g103380	99.153	118	1	0	1	118	1	118	3.74e-84	241
MS.gene36231.t1	MTR_4g011060	60.684	117	34	2	1	117	1	105	6.50e-41	132
MS.gene36231.t1	MTR_4g114050	43.548	124	41	5	1	118	1	101	3.25e-23	86.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36231.t1	AT1G80180	48.551	138	51	6	1	118	1	138	1.34e-30	106
MS.gene36231.t1	AT1G15400	49.180	122	44	5	1	105	1	121	9.27e-29	102
MS.gene36231.t1	AT1G15400	49.180	122	44	5	1	105	1	121	1.10e-28	101
MS.gene36231.t1	AT1G15400	49.180	122	44	5	1	105	1	121	1.54e-28	101
MS.gene36231.t1	AT5G20100	47.273	110	42	5	3	108	2	99	1.27e-18	75.5

Find 29 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGTTCATCAGGGTTTGTT+TGG	0.296640	2.1:-2447178	MS.gene36231:CDS
TTCAGAAGACAAGGTTCATC+AGG	0.369694	2.1:-2447188	MS.gene36231:CDS
GTTCTTGTTGATTCTGACTC+TGG	0.396706	2.1:+2447117	None:intergenic
AGGTTCATCAGGGTTTGTTT+GGG	0.404671	2.1:-2447177	MS.gene36231:CDS
CTCCTAGGATTTCTGCCTGT+GGG	0.417892	2.1:-2446947	MS.gene36231:CDS
CTTCTCTGAACAGTATTGAT+TGG	0.435131	2.1:+2447035	None:intergenic
CGTTCTAACGGTGGAGGACG+TGG	0.452280	2.1:-2447011	MS.gene36231:CDS
AACTACCTTGATCGATGCTT+AGG	0.486228	2.1:+2446882	None:intergenic
TTGGGAAAGTAGGGGAGAAG+AGG	0.507939	2.1:-2446911	MS.gene36231:CDS
CGATTGAGCCACCGTCTCCT+AGG	0.529012	2.1:-2446962	MS.gene36231:CDS
AACCCACAGGCAGAAATCCT+AGG	0.546823	2.1:+2446945	None:intergenic
GGAGACGGTGGCTCAATCGC+CGG	0.558530	2.1:+2446966	None:intergenic
CCGTACCGGAAAAGTTTCTC+CGG	0.558933	2.1:-2446985	MS.gene36231:CDS
AAAAGCACCACAAAACCCAC+AGG	0.561933	2.1:+2446932	None:intergenic
GATGCAACATCACCACAGCT+TGG	0.569916	2.1:-2447059	MS.gene36231:CDS
GGAGGACGTGGTTACCGTAC+CGG	0.573123	2.1:-2446999	MS.gene36231:CDS
TCTCCTAGGATTTCTGCCTG+TGG	0.574828	2.1:-2446948	MS.gene36231:CDS
AACCACCTAAGCATCGATCA+AGG	0.579242	2.1:-2446887	MS.gene36231:CDS
TCAGAAGACAAGGTTCATCA+GGG	0.600967	2.1:-2447187	MS.gene36231:CDS
TACCTTGATCGATGCTTAGG+TGG	0.600976	2.1:+2446885	None:intergenic
AGAAGTCGTTCTAACGGTGG+AGG	0.607642	2.1:-2447017	MS.gene36231:CDS
ACAGAGAAACCAGACATGGA+AGG	0.634834	2.1:-2447233	None:intergenic
GTTCAGAGAAGTCGTTCTAA+CGG	0.637358	2.1:-2447023	MS.gene36231:CDS
CAGGCAGAAATCCTAGGAGA+CGG	0.637647	2.1:+2446951	None:intergenic
GCAGTATCATTCAGAAGACA+AGG	0.648817	2.1:-2447197	MS.gene36231:CDS
CAGAGAAGTCGTTCTAACGG+TGG	0.679356	2.1:-2447020	MS.gene36231:CDS
GCAGAAATCCTAGGAGACGG+TGG	0.696269	2.1:+2446954	None:intergenic
GCTGTGGTGATGTTGCATCA+CGG	0.699454	2.1:+2447063	None:intergenic
GTATTGATTGGTCCAAGCTG+TGG	0.700900	2.1:+2447047	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTTCTCTGAACAGTATTGAT+TGG	+	chr2.1:2447070-2447089	None:intergenic	35.0%
	GCAGTATCATTCAGAAGACA+AGG	-	chr2.1:2446905-2446924	MS.gene36231:CDS	40.0%
	GTTCAGAGAAGTCGTTCTAA+CGG	-	chr2.1:2447079-2447098	MS.gene36231:CDS	40.0%
	TCAGAAGACAAGGTTCATCA+GGG	-	chr2.1:2446915-2446934	MS.gene36231:CDS	40.0%
	TTCAGAAGACAAGGTTCATC+AGG	-	chr2.1:2446914-2446933	MS.gene36231:CDS	40.0%
!	AAGGTTCATCAGGGTTTGTT+TGG	-	chr2.1:2446924-2446943	MS.gene36231:CDS	40.0%
!	AGGTTCATCAGGGTTTGTTT+GGG	-	chr2.1:2446925-2446944	MS.gene36231:CDS	40.0%
!!	GTTCTTGTTGATTCTGACTC+TGG	+	chr2.1:2446988-2447007	None:intergenic	40.0%
	TACCTTGATCGATGCTTAGG+TGG	+	chr2.1:2447220-2447239	None:intergenic	45.0%
!	AATCGCCGGAGAAACTTTTC+CGG	+	chr2.1:2447125-2447144	None:intergenic	45.0%
!	ATTTCTGCCTGTGGGTTTTG+TGG	-	chr2.1:2447163-2447182	MS.gene36231:CDS	45.0%
!!	AAAAGCACCACAAAACCCAC+AGG	+	chr2.1:2447173-2447192	None:intergenic	45.0%
!!	AACCACCTAAGCATCGATCA+AGG	-	chr2.1:2447215-2447234	MS.gene36231:CDS	45.0%
!!	GTATTGATTGGTCCAAGCTG+TGG	+	chr2.1:2447058-2447077	None:intergenic	45.0%
!!	GTGGGTTTTGTGGTGCTTTT+GGG	-	chr2.1:2447173-2447192	MS.gene36231:CDS	45.0%
!!	GTGGTGCTTTTGGGAAAGTA+GGG	-	chr2.1:2447182-2447201	MS.gene36231:CDS	45.0%
!!	TGGTGCTTTTGGGAAAGTAG+GGG	-	chr2.1:2447183-2447202	MS.gene36231:CDS	45.0%
!!	TGTGGGTTTTGTGGTGCTTT+TGG	-	chr2.1:2447172-2447191	MS.gene36231:CDS	45.0%
!!	TGTGGTGCTTTTGGGAAAGT+AGG	-	chr2.1:2447181-2447200	MS.gene36231:CDS	45.0%
	AACCCACAGGCAGAAATCCT+AGG	+	chr2.1:2447160-2447179	None:intergenic	50.0%
	AGAAGTCGTTCTAACGGTGG+AGG	-	chr2.1:2447085-2447104	MS.gene36231:CDS	50.0%
	CAGAGAAGTCGTTCTAACGG+TGG	-	chr2.1:2447082-2447101	MS.gene36231:CDS	50.0%
	CAGGCAGAAATCCTAGGAGA+CGG	+	chr2.1:2447154-2447173	None:intergenic	50.0%
	CCGTACCGGAAAAGTTTCTC+CGG	-	chr2.1:2447117-2447136	MS.gene36231:CDS	50.0%
	CTCCTAGGATTTCTGCCTGT+GGG	-	chr2.1:2447155-2447174	MS.gene36231:CDS	50.0%
	GATGCAACATCACCACAGCT+TGG	-	chr2.1:2447043-2447062	MS.gene36231:CDS	50.0%
	TCTCCTAGGATTTCTGCCTG+TGG	-	chr2.1:2447154-2447173	MS.gene36231:CDS	50.0%
	TTGGGAAAGTAGGGGAGAAG+AGG	-	chr2.1:2447191-2447210	MS.gene36231:CDS	50.0%
!	CCGGAGAAACTTTTCCGGTA+CGG	+	chr2.1:2447120-2447139	None:intergenic	50.0%
!	GCTGTGGTGATGTTGCATCA+CGG	+	chr2.1:2447042-2447061	None:intergenic	50.0%
	GCAGAAATCCTAGGAGACGG+TGG	+	chr2.1:2447151-2447170	None:intergenic	55.0%
	CGATTGAGCCACCGTCTCCT+AGG	-	chr2.1:2447140-2447159	MS.gene36231:CDS	60.0%
	CGTTCTAACGGTGGAGGACG+TGG	-	chr2.1:2447091-2447110	MS.gene36231:CDS	60.0%
	GGAGGACGTGGTTACCGTAC+CGG	-	chr2.1:2447103-2447122	MS.gene36231:CDS	60.0%
	GGAGACGGTGGCTCAATCGC+CGG	+	chr2.1:2447139-2447158	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	2446884	2447240	2446884	ID=MS.gene36231
chr2.1	mRNA	2446884	2447240	2446884	ID=MS.gene36231.t1;Parent=MS.gene36231
chr2.1	exon	2446884	2447240	2446884	ID=MS.gene36231.t1.exon1;Parent=MS.gene36231.t1
chr2.1	CDS	2446884	2447240	2446884	ID=cds.MS.gene36231.t1;Parent=MS.gene36231.t1

Gene Sequence	>MS.gene36231 ATGGAAGGTTTACAAAGATCTGCAGTATCATTCAGAAGACAAGGTTCATCAGGGTTTGTTTGGGATGATAGATTTCTACAAGAAGAAATAAACAAGATGAACCAGAGTCAGAATCAACAAGAACAAGAAGACAAAGATGAAATCAAAGAGATGAACCGTGATGCAACATCACCACAGCTTGGACCAATCAATACTGTTCAGAGAAGTCGTTCTAACGGTGGAGGACGTGGTTACCGTACCGGAAAAGTTTCTCCGGCGATTGAGCCACCGTCTCCTAGGATTTCTGCCTGTGGGTTTTGTGGTGCTTTTGGGAAAGTAGGGGAGAAGAGGAAACCACCTAAGCATCGATCAAGGTAG
Protein sequence	>MS.gene36231.t1 MEGLQRSAVSFRRQGSSGFVWDDRFLQEEINKMNQSQNQQEQEDKDEIKEMNRDATSPQLGPINTVQRSRSNGGGRGYRTGKVSPAIEPPSPRISACGFCGAFGKVGEKRKPPKHRSR