Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36229.t1 | XP_003597862.2 | 99.2 | 118 | 1 | 0 | 1 | 118 | 1 | 118 | 5.70E-52 | 213.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36229.t1 | Q9SSC1 | 46.4 | 138 | 54 | 6 | 1 | 118 | 1 | 138 | 7.7e-21 | 101.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36229.t1 | Q2HVW4 | 99.2 | 118 | 1 | 0 | 1 | 118 | 1 | 118 | 4.1e-52 | 213.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene36229 | 0.824814 | 6.58E-54 | -1.69E-46 |
| MS.gene049254 | MS.gene36229 | 0.809161 | 2.17E-50 | -1.69E-46 |
| MS.gene049293 | MS.gene36229 | -0.805887 | 1.07E-49 | -1.69E-46 |
| MS.gene049378 | MS.gene36229 | 0.853438 | 2.39E-61 | -1.69E-46 |
| MS.gene049419 | MS.gene36229 | -0.802926 | 4.45E-49 | -1.69E-46 |
| MS.gene049669 | MS.gene36229 | 0.854791 | 9.73E-62 | -1.69E-46 |
| MS.gene050026 | MS.gene36229 | 0.802597 | 5.20E-49 | -1.69E-46 |
| MS.gene050105 | MS.gene36229 | 0.815738 | 7.93E-52 | -1.69E-46 |
| MS.gene050227 | MS.gene36229 | 0.814626 | 1.40E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene36229 | 0.813659 | 2.29E-51 | -1.69E-46 |
| MS.gene050312 | MS.gene36229 | 0.810027 | 1.41E-50 | -1.69E-46 |
| MS.gene050355 | MS.gene36229 | 0.813142 | 2.97E-51 | -1.69E-46 |
| MS.gene050750 | MS.gene36229 | 0.825257 | 5.17E-54 | -1.69E-46 |
| MS.gene050953 | MS.gene36229 | 0.826575 | 2.52E-54 | -1.69E-46 |
| MS.gene051064 | MS.gene36229 | 0.841485 | 4.62E-58 | -1.69E-46 |
| MS.gene051332 | MS.gene36229 | 0.800366 | 1.49E-48 | -1.69E-46 |
| MS.gene051368 | MS.gene36229 | 0.845271 | 4.51E-59 | -1.69E-46 |
| MS.gene051832 | MS.gene36229 | 0.810914 | 9.09E-51 | -1.69E-46 |
| MS.gene051926 | MS.gene36229 | -0.805791 | 1.13E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene36229 | 0.840869 | 6.71E-58 | -1.69E-46 |
| MS.gene052333 | MS.gene36229 | 0.826342 | 2.86E-54 | -1.69E-46 |
| MS.gene052389 | MS.gene36229 | 0.854953 | 8.73E-62 | -1.69E-46 |
| MS.gene052582 | MS.gene36229 | 0.813731 | 2.21E-51 | -1.69E-46 |
| MS.gene052584 | MS.gene36229 | 0.801419 | 9.08E-49 | -1.69E-46 |
| MS.gene052586 | MS.gene36229 | 0.880259 | 6.36E-70 | -1.69E-46 |
| MS.gene052685 | MS.gene36229 | 0.820955 | 5.22E-53 | -1.69E-46 |
| MS.gene052954 | MS.gene36229 | 0.803513 | 3.36E-49 | -1.69E-46 |
| MS.gene053006 | MS.gene36229 | 0.81275 | 3.62E-51 | -1.69E-46 |
| MS.gene053340 | MS.gene36229 | 0.813117 | 3.01E-51 | -1.69E-46 |
| MS.gene05365 | MS.gene36229 | 0.808498 | 3.01E-50 | -1.69E-46 |
| MS.gene053963 | MS.gene36229 | 0.830944 | 2.21E-55 | -1.69E-46 |
| MS.gene05415 | MS.gene36229 | 0.80896 | 2.40E-50 | -1.69E-46 |
| MS.gene054222 | MS.gene36229 | 0.827422 | 1.58E-54 | -1.69E-46 |
| MS.gene054291 | MS.gene36229 | -0.839952 | 1.16E-57 | -1.69E-46 |
| MS.gene054405 | MS.gene36229 | 0.816626 | 5.02E-52 | -1.69E-46 |
| MS.gene054468 | MS.gene36229 | 0.830243 | 3.28E-55 | -1.69E-46 |
| MS.gene054479 | MS.gene36229 | 0.803652 | 3.15E-49 | -1.69E-46 |
| MS.gene054576 | MS.gene36229 | 0.819962 | 8.81E-53 | -1.69E-46 |
| MS.gene054657 | MS.gene36229 | 0.803952 | 2.73E-49 | -1.69E-46 |
| MS.gene055225 | MS.gene36229 | 0.827262 | 1.72E-54 | -1.69E-46 |
| MS.gene055586 | MS.gene36229 | 0.817241 | 3.65E-52 | -1.69E-46 |
| MS.gene055701 | MS.gene36229 | 0.851613 | 7.92E-61 | -1.69E-46 |
| MS.gene056064 | MS.gene36229 | 0.803127 | 4.04E-49 | -1.69E-46 |
| MS.gene056068 | MS.gene36229 | 0.801196 | 1.01E-48 | -1.69E-46 |
| MS.gene056506 | MS.gene36229 | 0.82395 | 1.05E-53 | -1.69E-46 |
| MS.gene056779 | MS.gene36229 | 0.802203 | 6.27E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene36229 | 0.855691 | 5.32E-62 | -1.69E-46 |
| MS.gene057156 | MS.gene36229 | -0.834646 | 2.66E-56 | -1.69E-46 |
| MS.gene057598 | MS.gene36229 | 0.800495 | 1.40E-48 | -1.69E-46 |
| MS.gene058037 | MS.gene36229 | 0.800763 | 1.24E-48 | -1.69E-46 |
| MS.gene058498 | MS.gene36229 | 0.844404 | 7.72E-59 | -1.69E-46 |
| MS.gene058500 | MS.gene36229 | 0.830203 | 3.35E-55 | -1.69E-46 |
| MS.gene058502 | MS.gene36229 | 0.828027 | 1.13E-54 | -1.69E-46 |
| MS.gene05954 | MS.gene36229 | 0.809171 | 2.16E-50 | -1.69E-46 |
| MS.gene059952 | MS.gene36229 | 0.8019 | 7.24E-49 | -1.69E-46 |
| MS.gene060012 | MS.gene36229 | 0.808125 | 3.61E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene36229 | 0.803529 | 3.34E-49 | -1.69E-46 |
| MS.gene060521 | MS.gene36229 | 0.803592 | 3.24E-49 | -1.69E-46 |
| MS.gene060572 | MS.gene36229 | 0.810878 | 9.26E-51 | -1.69E-46 |
| MS.gene060879 | MS.gene36229 | 0.990372 | 2.52E-182 | -1.69E-46 |
| MS.gene061040 | MS.gene36229 | -0.84423 | 8.60E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene36229 | MS.gene36231 | PPI |
| MS.gene36229 | MS.gene026783 | PPI |
| MS.gene36229 | MS.gene60230 | PPI |
| MS.gene36229 | MS.gene034054 | PPI |
| MS.gene36229 | MS.gene060879 | PPI |
| MS.gene36229 | MS.gene86369 | PPI |
| MS.gene36229 | MS.gene75382 | PPI |
| MS.gene36229 | MS.gene89049 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36229.t1 | MTR_2g103380 | 99.153 | 118 | 1 | 0 | 1 | 118 | 1 | 118 | 3.74e-84 | 241 |
| MS.gene36229.t1 | MTR_4g011060 | 60.684 | 117 | 34 | 2 | 1 | 117 | 1 | 105 | 6.50e-41 | 132 |
| MS.gene36229.t1 | MTR_4g114050 | 43.548 | 124 | 41 | 5 | 1 | 118 | 1 | 101 | 3.25e-23 | 86.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36229.t1 | AT1G80180 | 48.551 | 138 | 51 | 6 | 1 | 118 | 1 | 138 | 1.34e-30 | 106 |
| MS.gene36229.t1 | AT1G15400 | 49.180 | 122 | 44 | 5 | 1 | 105 | 1 | 121 | 9.27e-29 | 102 |
| MS.gene36229.t1 | AT1G15400 | 49.180 | 122 | 44 | 5 | 1 | 105 | 1 | 121 | 1.10e-28 | 101 |
| MS.gene36229.t1 | AT1G15400 | 49.180 | 122 | 44 | 5 | 1 | 105 | 1 | 121 | 1.54e-28 | 101 |
| MS.gene36229.t1 | AT5G20100 | 47.273 | 110 | 42 | 5 | 3 | 108 | 2 | 99 | 1.27e-18 | 75.5 |
Find 0 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTTCTCTGAACAGTATTGAT+TGG | + | 15689:19970-19989 | None:intergenic | 35.0% |
| GCAGTATCATTCAGAAGACA+AGG | - | 15689:19805-19824 | MS.gene36229:CDS | 40.0% | |
| GTTCAGAGAAGTCGTTCTAA+CGG | - | 15689:19979-19998 | MS.gene36229:CDS | 40.0% | |
| TCAGAAGACAAGGTTCATCA+GGG | - | 15689:19815-19834 | MS.gene36229:CDS | 40.0% | |
| TTCAGAAGACAAGGTTCATC+AGG | - | 15689:19814-19833 | MS.gene36229:CDS | 40.0% | |
| ! | AAGGTTCATCAGGGTTTGTT+TGG | - | 15689:19824-19843 | MS.gene36229:CDS | 40.0% |
| ! | AGGTTCATCAGGGTTTGTTT+GGG | - | 15689:19825-19844 | MS.gene36229:CDS | 40.0% |
| !! | GTTCTTGTTGATTCTGACTC+TGG | + | 15689:19888-19907 | None:intergenic | 40.0% |
| TACCTTGATCGATGCTTAGG+TGG | + | 15689:20120-20139 | None:intergenic | 45.0% | |
| ! | AATCGCCGGAGAAACTTTTC+CGG | + | 15689:20025-20044 | None:intergenic | 45.0% |
| ! | ATTTCTGCCTGTGGGTTTTG+TGG | - | 15689:20063-20082 | MS.gene36229:CDS | 45.0% |
| !! | AAAAGCACCACAAAACCCAC+AGG | + | 15689:20073-20092 | None:intergenic | 45.0% |
| !! | AACCACCTAAGCATCGATCA+AGG | - | 15689:20115-20134 | MS.gene36229:CDS | 45.0% |
| !! | GTATTGATTGGTCCAAGCTG+TGG | + | 15689:19958-19977 | None:intergenic | 45.0% |
| !! | GTGGGTTTTGTGGTGCTTTT+GGG | - | 15689:20073-20092 | MS.gene36229:CDS | 45.0% |
| !! | GTGGTGCTTTTGGGAAAGTA+GGG | - | 15689:20082-20101 | MS.gene36229:CDS | 45.0% |
| !! | TGGTGCTTTTGGGAAAGTAG+GGG | - | 15689:20083-20102 | MS.gene36229:CDS | 45.0% |
| !! | TGTGGGTTTTGTGGTGCTTT+TGG | - | 15689:20072-20091 | MS.gene36229:CDS | 45.0% |
| !! | TGTGGTGCTTTTGGGAAAGT+AGG | - | 15689:20081-20100 | MS.gene36229:CDS | 45.0% |
| AACCCACAGGCAGAAATCCT+AGG | + | 15689:20060-20079 | None:intergenic | 50.0% | |
| AGAAGTCGTTCTAACGGTGG+AGG | - | 15689:19985-20004 | MS.gene36229:CDS | 50.0% | |
| CAGAGAAGTCGTTCTAACGG+TGG | - | 15689:19982-20001 | MS.gene36229:CDS | 50.0% | |
| CAGGCAGAAATCCTAGGAGA+CGG | + | 15689:20054-20073 | None:intergenic | 50.0% | |
| CCGTACCGGAAAAGTTTCTC+CGG | - | 15689:20017-20036 | MS.gene36229:CDS | 50.0% | |
| CTCCTAGGATTTCTGCCTGT+GGG | - | 15689:20055-20074 | MS.gene36229:CDS | 50.0% | |
| GATGCAACATCACCACAGCT+TGG | - | 15689:19943-19962 | MS.gene36229:CDS | 50.0% | |
| TCTCCTAGGATTTCTGCCTG+TGG | - | 15689:20054-20073 | MS.gene36229:CDS | 50.0% | |
| TTGGGAAAGTAGGGGAGAAG+AGG | - | 15689:20091-20110 | MS.gene36229:CDS | 50.0% | |
| ! | CCGGAGAAACTTTTCCGGTA+CGG | + | 15689:20020-20039 | None:intergenic | 50.0% |
| ! | GCTGTGGTGATGTTGCATCA+CGG | + | 15689:19942-19961 | None:intergenic | 50.0% |
| GCAGAAATCCTAGGAGACGG+TGG | + | 15689:20051-20070 | None:intergenic | 55.0% | |
| CGATTGAGCCACCGTCTCCT+AGG | - | 15689:20040-20059 | MS.gene36229:CDS | 60.0% | |
| CGTTCTAACGGTGGAGGACG+TGG | - | 15689:19991-20010 | MS.gene36229:CDS | 60.0% | |
| GGAGGACGTGGTTACCGTAC+CGG | - | 15689:20003-20022 | MS.gene36229:CDS | 60.0% | |
| GGAGACGGTGGCTCAATCGC+CGG | + | 15689:20039-20058 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15689 | gene | 19784 | 20140 | 19784 | ID=MS.gene36229 |
| 15689 | mRNA | 19784 | 20140 | 19784 | ID=MS.gene36229.t1;Parent=MS.gene36229 |
| 15689 | exon | 19784 | 20140 | 19784 | ID=MS.gene36229.t1.exon1;Parent=MS.gene36229.t1 |
| 15689 | CDS | 19784 | 20140 | 19784 | ID=cds.MS.gene36229.t1;Parent=MS.gene36229.t1 |
| Gene Sequence |
| Protein sequence |
