Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027150.t1 | AES70281.2 | 59.1 | 379 | 94 | 4 | 440 | 788 | 2 | 349 | 6.00E-88 | 335.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027150.t1 | Q7XTT4 | 52.8 | 106 | 49 | 1 | 682 | 786 | 435 | 540 | 1.6e-25 | 119.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027150.t1 | G7J1Z7 | 59.1 | 379 | 94 | 4 | 440 | 788 | 2 | 349 | 4.4e-88 | 335.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene027150.t1 | TR | GNAT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene027150 | MS.gene02437 | PPI |
| MS.gene027150 | MS.gene007219 | PPI |
| MS.gene027150 | MS.gene033532 | PPI |
| MS.gene43653 | MS.gene027150 | PPI |
| MS.gene027150 | MS.gene022751 | PPI |
| MS.gene027150 | MS.gene57987 | PPI |
| MS.gene027150 | MS.gene050316 | PPI |
| MS.gene027150 | MS.gene58223 | PPI |
| MS.gene027150 | MS.gene024088 | PPI |
| MS.gene027150 | MS.gene007221 | PPI |
| MS.gene027150 | MS.gene041376 | PPI |
| MS.gene39295 | MS.gene027150 | PPI |
| MS.gene027150 | MS.gene020216 | PPI |
| MS.gene34516 | MS.gene027150 | PPI |
| MS.gene024089 | MS.gene027150 | PPI |
| MS.gene64679 | MS.gene027150 | PPI |
| MS.gene027150 | MS.gene010536 | PPI |
| MS.gene036674 | MS.gene027150 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027150.t1 | MTR_3g050860 | 62.402 | 383 | 75 | 5 | 440 | 788 | 2 | 349 | 1.57e-146 | 439 |
| MS.gene027150.t1 | MTR_3g050860 | 56.233 | 361 | 129 | 5 | 1 | 357 | 1 | 336 | 9.95e-111 | 345 |
| MS.gene027150.t1 | MTR_0439s0020 | 74.648 | 142 | 35 | 1 | 1 | 141 | 1 | 142 | 4.52e-65 | 215 |
| MS.gene027150.t1 | MTR_0439s0020 | 59.155 | 142 | 56 | 2 | 441 | 581 | 3 | 143 | 1.44e-46 | 163 |
| MS.gene027150.t1 | MTR_4g074930 | 75.000 | 104 | 26 | 0 | 677 | 780 | 311 | 414 | 4.02e-45 | 172 |
| MS.gene027150.t1 | MTR_4g074930 | 50.000 | 118 | 50 | 2 | 278 | 390 | 330 | 443 | 1.36e-22 | 103 |
| MS.gene027150.t1 | MTR_4g074930 | 75.000 | 104 | 26 | 0 | 677 | 780 | 346 | 449 | 4.52e-45 | 173 |
| MS.gene027150.t1 | MTR_4g074930 | 50.000 | 118 | 50 | 2 | 278 | 390 | 365 | 478 | 1.26e-22 | 103 |
| MS.gene027150.t1 | MTR_4g074930 | 80.000 | 50 | 10 | 0 | 215 | 264 | 11 | 60 | 1.16e-13 | 75.5 |
| MS.gene027150.t1 | MTR_4g074930 | 75.000 | 104 | 26 | 0 | 677 | 780 | 323 | 426 | 4.55e-45 | 172 |
| MS.gene027150.t1 | MTR_4g074930 | 50.000 | 118 | 50 | 2 | 278 | 390 | 342 | 455 | 1.37e-22 | 103 |
| MS.gene027150.t1 | MTR_4g074930 | 75.000 | 104 | 26 | 0 | 677 | 780 | 358 | 461 | 5.08e-45 | 173 |
| MS.gene027150.t1 | MTR_4g074930 | 50.000 | 118 | 50 | 2 | 278 | 390 | 377 | 490 | 1.27e-22 | 103 |
| MS.gene027150.t1 | MTR_4g074930 | 80.000 | 50 | 10 | 0 | 215 | 264 | 11 | 60 | 1.17e-13 | 75.5 |
| MS.gene027150.t1 | MTR_1g036430 | 48.515 | 101 | 51 | 1 | 679 | 778 | 276 | 376 | 1.53e-23 | 107 |
| MS.gene027150.t1 | MTR_1g036430 | 32.609 | 230 | 110 | 6 | 598 | 782 | 20 | 249 | 2.11e-23 | 106 |
| MS.gene027150.t1 | MTR_3g034200 | 36.975 | 119 | 69 | 5 | 477 | 592 | 42 | 157 | 2.75e-12 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene027150.t1 | AT3G18610 | 52.830 | 106 | 47 | 2 | 675 | 780 | 305 | 407 | 6.41e-27 | 117 |
| MS.gene027150.t1 | AT3G18610 | 44.068 | 118 | 53 | 3 | 278 | 390 | 325 | 434 | 4.45e-17 | 86.3 |
| MS.gene027150.t1 | AT3G18610 | 52.830 | 106 | 47 | 2 | 675 | 780 | 335 | 437 | 7.15e-27 | 117 |
| MS.gene027150.t1 | AT3G18610 | 44.068 | 118 | 53 | 3 | 278 | 390 | 355 | 464 | 3.98e-17 | 86.7 |
| MS.gene027150.t1 | AT3G18610 | 52.830 | 106 | 47 | 2 | 675 | 780 | 365 | 467 | 8.02e-27 | 117 |
| MS.gene027150.t1 | AT3G18610 | 44.068 | 118 | 53 | 3 | 278 | 390 | 385 | 494 | 3.62e-17 | 86.7 |
| MS.gene027150.t1 | AT1G48920 | 42.500 | 120 | 61 | 3 | 675 | 787 | 278 | 396 | 2.55e-24 | 108 |
| MS.gene027150.t1 | AT1G48920 | 36.975 | 119 | 69 | 2 | 278 | 390 | 298 | 416 | 4.37e-13 | 73.6 |
| MS.gene027150.t1 | AT1G45100 | 46.053 | 76 | 41 | 0 | 695 | 770 | 247 | 322 | 4.73e-11 | 67.0 |
Find 243 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATATCTTGCATAACGAATTT+TGG | 0.090281 | 3.4:-47852604 | None:intergenic |
| CATAAGGATGGAAAGCTTAA+AGG | 0.173752 | 3.4:+47855540 | MS.gene027150:CDS |
| GAAGCTGTTTCTTCTAAAAT+AGG | 0.182499 | 3.4:+47852365 | MS.gene027150:CDS |
| TAAAGATCGTCTTTCTTTAC+AGG | 0.196172 | 3.4:-47854777 | None:intergenic |
| CAAGGATATCCTCTTATTAT+TGG | 0.202894 | 3.4:+47851375 | MS.gene027150:CDS |
| AGTAATTACCTGTTATTATT+AGG | 0.205492 | 3.4:-47855790 | None:intergenic |
| GAAGAAACAGCTTCTGCTTC+AGG | 0.224450 | 3.4:-47852356 | None:intergenic |
| AAGTGAAGTTATTACATCTT+AGG | 0.242413 | 3.4:+47849194 | MS.gene027150:CDS |
| GGAGGCTTTGTCAGGGGTTT+TGG | 0.242686 | 3.4:+47851525 | MS.gene027150:CDS |
| TTACTTCATGGACTGTTTAC+AGG | 0.256586 | 3.4:+47855939 | MS.gene027150:CDS |
| GAACCTTAGTTCGACCATTT+TGG | 0.260174 | 3.4:-47857743 | None:intergenic |
| GACATCCTCGGTGGACGTTT+GGG | 0.271271 | 3.4:-47857827 | None:intergenic |
| AATGCGTCAGTGTTGGTCTT+TGG | 0.291887 | 3.4:-47848765 | None:intergenic |
| TGACATCCTCGGTGGACGTT+TGG | 0.301947 | 3.4:-47857828 | None:intergenic |
| ATTGCCGTCATTGTTGATTC+AGG | 0.320712 | 3.4:-47857658 | None:intergenic |
| AAGCTGTTACTTCTAAAATA+AGG | 0.322995 | 3.4:+47849366 | MS.gene027150:CDS |
| ATGTACCTTAGGCCGCAAAT+AGG | 0.323473 | 3.4:+47857709 | MS.gene027150:CDS |
| TGTATTCCTCTGGCTAATCT+AGG | 0.324507 | 3.4:-47857778 | None:intergenic |
| AGAGGATATCCTTGAGGATA+TGG | 0.327826 | 3.4:-47851366 | None:intergenic |
| AATACAACATTAGAAGAAAC+AGG | 0.329306 | 3.4:-47855909 | None:intergenic |
| TCATCTTCTGAAGAATCTTC+TGG | 0.335928 | 3.4:+47854804 | MS.gene027150:CDS |
| TGTTGACCTATATAAGATTC+TGG | 0.336459 | 3.4:+47848312 | MS.gene027150:CDS |
| ACTCCTTCTCTCCGAAAGAT+AGG | 0.337370 | 3.4:-47852849 | None:intergenic |
| TGTACCTTAGGCCGCAAATA+GGG | 0.347736 | 3.4:+47857710 | MS.gene027150:CDS |
| GTTGATGTATGTCTTGGTTA+TGG | 0.353092 | 3.4:+47851114 | MS.gene027150:CDS |
| TGACGTGGATCTTGAAAGTT+TGG | 0.353226 | 3.4:+47852701 | MS.gene027150:CDS |
| GAGGATATGGACGCCACTCC+CGG | 0.354161 | 3.4:-47851353 | None:intergenic |
| TTCTACAACGATGGTGTCCC+CGG | 0.363342 | 3.4:+47848264 | None:intergenic |
| ATGTCGAGAGCTTACGCGTT+TGG | 0.366733 | 3.4:-47851193 | None:intergenic |
| ACTTGTGACCATGGCTGAAA+TGG | 0.368546 | 3.4:+47848531 | MS.gene027150:CDS |
| AAGCTGTTTCTTCTAAAATA+GGG | 0.371461 | 3.4:+47852366 | MS.gene027150:CDS |
| GTGGCGGAGGAGGCTTTGTC+AGG | 0.376610 | 3.4:+47851517 | MS.gene027150:CDS |
| ATTACTCTTCTTCGAAAAGA+TGG | 0.379614 | 3.4:+47848785 | MS.gene027150:CDS |
| ATGTCAAGAGCTTACGTATT+GGG | 0.379625 | 3.4:-47855598 | None:intergenic |
| ATCCATCAGAGTTGGTCTTC+GGG | 0.381778 | 3.4:-47853159 | None:intergenic |
| ATGCGTCAGTGTTGGTCTTT+GGG | 0.387142 | 3.4:-47848764 | None:intergenic |
| CGTTTGGGGCGGGTTGTTGT+CGG | 0.387498 | 3.4:-47857812 | None:intergenic |
| CATATACTCCTAATAATAAC+AGG | 0.390293 | 3.4:+47855782 | MS.gene027150:CDS |
| TCACTTCACGAGGCTCGTTC+AGG | 0.391717 | 3.4:-47849177 | None:intergenic |
| CAACAACCATTCCACAATCT+TGG | 0.392218 | 3.4:-47851095 | None:intergenic |
| GAAGTAACAGCTTCTGCTGC+AGG | 0.395666 | 3.4:-47849356 | None:intergenic |
| GATGGAAAGCTTAAAGGCTT+TGG | 0.397596 | 3.4:+47855546 | MS.gene027150:CDS |
| TATTAGGCACTTAAGATGAA+TGG | 0.404097 | 3.4:+47855706 | MS.gene027150:intron |
| AATAGGCACTTGATAAGAAT+GGG | 0.408077 | 3.4:+47851292 | MS.gene027150:intron |
| ATTACTCTTCTTCGAAAAGA+TGG | 0.410597 | 3.4:+47853180 | MS.gene027150:CDS |
| TGACGTGGATACTCAAAGTT+TGG | 0.414598 | 3.4:+47848351 | MS.gene027150:CDS |
| GCGTTTCTTAAGACCACATC+CGG | 0.415976 | 3.4:-47852633 | None:intergenic |
| GGCCCTATCTTTCGAAGAAA+AGG | 0.417511 | 3.4:+47848496 | MS.gene027150:CDS |
| AGGAAAATATACCTTGGTAT+AGG | 0.418282 | 3.4:-47855985 | None:intergenic |
| CAAAGTTCGAACTAAGGTTC+TGG | 0.418284 | 3.4:+47857606 | MS.gene027150:CDS |
| TCTTCGTTAACCCTATCTTT+CGG | 0.418866 | 3.4:+47852838 | MS.gene027150:CDS |
| GTGAAGAGCAATAGTATTGA+TGG | 0.420089 | 3.4:+47848825 | MS.gene027150:CDS |
| AATCCATCAGAGTTGGTCTT+CGG | 0.430864 | 3.4:-47853160 | None:intergenic |
| TGTACGTCTTGCTTCTCATA+AGG | 0.432229 | 3.4:+47855524 | MS.gene027150:CDS |
| GTGAAGAGCAATTATATTGA+TGG | 0.434863 | 3.4:+47853220 | MS.gene027150:CDS |
| AGGGGTCAATAAAGCCAAAA+TGG | 0.434881 | 3.4:+47857729 | MS.gene027150:CDS |
| AACGATGGTGTCCCCGGGTG+TGG | 0.437378 | 3.4:+47848270 | None:intergenic |
| AGAATCTTATATAGGTCAAC+AGG | 0.439517 | 3.4:-47848310 | None:intergenic |
| CCTCGGTGGACGTTTGGGGC+GGG | 0.440085 | 3.4:-47857822 | None:intergenic |
| AGTGAAGTTATTACATCTTA+GGG | 0.441387 | 3.4:+47849195 | MS.gene027150:CDS |
| GGCTGAAATGGACTTGGAAA+AGG | 0.444198 | 3.4:+47848543 | MS.gene027150:CDS |
| AAGAAACAGCTTCTGCTTCA+GGG | 0.451779 | 3.4:-47852355 | None:intergenic |
| CATATCACTTCCCTCTTCGT+TGG | 0.453367 | 3.4:+47848475 | MS.gene027150:CDS |
| TAATAGGCACTTGATAAGAA+TGG | 0.456193 | 3.4:+47851291 | MS.gene027150:intron |
| GACTTGATTTAGCTCGGGAG+AGG | 0.463792 | 3.4:+47855755 | MS.gene027150:CDS |
| TGTTGGGAACTTATCATTCA+AGG | 0.465531 | 3.4:+47850967 | MS.gene027150:CDS |
| TGAAAAGGACTTGAGGGATA+AGG | 0.466500 | 3.4:+47852896 | MS.gene027150:CDS |
| GCTTTATTGACCCCTATTTG+CGG | 0.467033 | 3.4:-47857721 | None:intergenic |
| ACTTGTGGAAAGCGCTGAAA+AGG | 0.467490 | 3.4:+47852881 | MS.gene027150:CDS |
| GAACTGAAGCGTACTGGTTA+CGG | 0.472216 | 3.4:+47848451 | MS.gene027150:CDS |
| AAAGATAGGGTTAACGAAGA+TGG | 0.472513 | 3.4:-47852835 | None:intergenic |
| ATTAGGCACTTAAGATGAAT+GGG | 0.473468 | 3.4:+47855707 | MS.gene027150:intron |
| TATGTTGGGAACTTATCCTT+CGG | 0.474371 | 3.4:+47855371 | MS.gene027150:CDS |
| CTTCGAAAAGATGGGTTATG+AGG | 0.474734 | 3.4:+47848794 | MS.gene027150:CDS |
| TCAGCTGAAAGTGGCGGAGG+AGG | 0.475866 | 3.4:+47851507 | MS.gene027150:CDS |
| CTTTCGAAGACAATATATGT+TGG | 0.475870 | 3.4:+47850950 | MS.gene027150:intron |
| GGTTACCTTGTCTTTGATGT+TGG | 0.476676 | 3.4:+47848427 | MS.gene027150:CDS |
| TAAGAGTAATGGAGAGGATG+AGG | 0.477121 | 3.4:+47851548 | MS.gene027150:CDS |
| TTTCGAAGACAATATATGTT+GGG | 0.477167 | 3.4:+47850951 | MS.gene027150:intron |
| TCAGAGTTGGTCTTCGGGAC+GGG | 0.477721 | 3.4:-47853154 | None:intergenic |
| TCTAATACCTCATCTTCATC+AGG | 0.477800 | 3.4:-47849223 | None:intergenic |
| AATTACCTTCCAATAATAAG+AGG | 0.478964 | 3.4:-47851384 | None:intergenic |
| GATCTTGAAAGTTTGGAGAA+TGG | 0.482941 | 3.4:+47852708 | MS.gene027150:CDS |
| GAGGTGTACCATGGATATCC+TGG | 0.484184 | 3.4:-47857555 | None:intergenic |
| TGCCAAACAAAAGGTATTGA+AGG | 0.484854 | 3.4:+47850762 | MS.gene027150:CDS |
| TGCCAAACAAAAGGTATTGA+AGG | 0.484854 | 3.4:+47854565 | MS.gene027150:CDS |
| TTACTCTTCTTCGAAAAGAT+GGG | 0.485859 | 3.4:+47853181 | MS.gene027150:CDS |
| TGACCCCTATTTGCGGCCTA+AGG | 0.493447 | 3.4:-47857714 | None:intergenic |
| GACTTCAAGAAGCCCGGGAG+TGG | 0.494287 | 3.4:+47851340 | MS.gene027150:CDS |
| TGGACTGTTTACAGGCGTGC+AGG | 0.495759 | 3.4:+47855947 | MS.gene027150:CDS |
| CGAGGTTGCTGCCAAACAAA+AGG | 0.496709 | 3.4:+47850753 | MS.gene027150:CDS |
| ATGGTTGTTGATGTATGTCT+TGG | 0.496788 | 3.4:+47851108 | MS.gene027150:CDS |
| CTTGATCATCTGTATTCCTC+TGG | 0.497518 | 3.4:-47857788 | None:intergenic |
| CTGTCCGACTTGATTTAGCT+CGG | 0.502487 | 3.4:+47855749 | MS.gene027150:CDS |
| AACCCTATCTTTCGGAGAGA+AGG | 0.502877 | 3.4:+47852846 | MS.gene027150:CDS |
| CGTCATTGTTGATTCAGGCA+TGG | 0.504135 | 3.4:-47857653 | None:intergenic |
| GTCAGTGTTGGTCTTTGGGA+TGG | 0.506964 | 3.4:-47848760 | None:intergenic |
| TTACTCTTCTTCGAAAAGAT+GGG | 0.507362 | 3.4:+47848786 | MS.gene027150:CDS |
| TCCTCGGTGGACGTTTGGGG+CGG | 0.508025 | 3.4:-47857823 | None:intergenic |
| AGTTTCTTCAAAGAAGCAAA+AGG | 0.509361 | 3.4:+47854529 | MS.gene027150:CDS |
| TTCCAAGTCCATTTCAGCCA+TGG | 0.509729 | 3.4:-47848539 | None:intergenic |
| GACCATGGCTGAAATGGACT+TGG | 0.509783 | 3.4:+47848537 | MS.gene027150:CDS |
| GTTCGAACTTTGCTCTCAGT+GGG | 0.510129 | 3.4:-47857595 | None:intergenic |
| CATTCACCAGAATCTTATAT+AGG | 0.510974 | 3.4:-47848318 | None:intergenic |
| AAGCCAAAATGGTCGAACTA+AGG | 0.512436 | 3.4:+47857740 | MS.gene027150:CDS |
| ACTTGATTTAGCTCGGGAGA+GGG | 0.513360 | 3.4:+47855756 | MS.gene027150:CDS |
| GTGTGAAGACATTATATGTT+GGG | 0.513802 | 3.4:+47855357 | MS.gene027150:CDS |
| TGACCACAACCATTACTCTT+CGG | 0.514416 | 3.4:-47852804 | None:intergenic |
| TGAGGTTGCTGCCAAACAAA+AGG | 0.514624 | 3.4:+47854556 | MS.gene027150:CDS |
| CGTGTGAAGACATTATATGT+TGG | 0.515977 | 3.4:+47855356 | MS.gene027150:CDS |
| AATGGTCGAACTAAGGTTCA+AGG | 0.517845 | 3.4:+47857747 | MS.gene027150:CDS |
| AATATACCTTGGTATAGGCC+TGG | 0.518085 | 3.4:-47855980 | None:intergenic |
| GCGTCTCTTACGACCACACC+CGG | 0.518110 | 3.4:-47848283 | None:intergenic |
| CGACATATTTAAGATTCTCG+TGG | 0.520223 | 3.4:+47852665 | MS.gene027150:CDS |
| TTCACAGGAGAAACAACAAC+AGG | 0.521749 | 3.4:-47849954 | None:intergenic |
| CTTTCATGAATCTGAGGATG+TGG | 0.522797 | 3.4:+47854613 | MS.gene027150:CDS |
| AGAACTATCAGCTTTCTTTG+CGG | 0.524386 | 3.4:-47849396 | None:intergenic |
| GATATCCTCTTATTATTGGA+AGG | 0.526173 | 3.4:+47851379 | MS.gene027150:CDS |
| AAGCGCTGAAAAGGACTTGA+GGG | 0.526550 | 3.4:+47852890 | MS.gene027150:CDS |
| TGTCCGACTTGATTTAGCTC+GGG | 0.530721 | 3.4:+47855750 | MS.gene027150:CDS |
| GGTAGTCAAAATTAGTTCGC+AGG | 0.532939 | 3.4:+47854586 | MS.gene027150:CDS |
| CGTCTTGCTTCTCATAAGGA+TGG | 0.533795 | 3.4:+47855528 | MS.gene027150:CDS |
| GATACTCAAAGTTTGGTGAA+TGG | 0.534335 | 3.4:+47848358 | MS.gene027150:CDS |
| AGTGACTTCAAAGAAGCAAA+AGG | 0.536343 | 3.4:+47850726 | MS.gene027150:CDS |
| TCAGTGTTGGTCTTTGGGAT+GGG | 0.537407 | 3.4:-47848759 | None:intergenic |
| GTCCCGAAGACCAACTCTGA+TGG | 0.538106 | 3.4:+47853157 | MS.gene027150:CDS |
| AGTTCGAACTTTGCTCTCAG+TGG | 0.541521 | 3.4:-47857596 | None:intergenic |
| GGATATCCATGGTACACCTC+AGG | 0.542579 | 3.4:+47857558 | MS.gene027150:CDS |
| TGAGAGCAAAGTTCGAACTA+AGG | 0.543002 | 3.4:+47857600 | MS.gene027150:CDS |
| CGCTACTCGACTTGTGACCA+TGG | 0.544412 | 3.4:+47848522 | MS.gene027150:CDS |
| CTTTGATGAATCTGAGGACG+TGG | 0.545887 | 3.4:+47850810 | MS.gene027150:CDS |
| ATGTCCGACTTCAAGAAGCC+CGG | 0.547348 | 3.4:+47851334 | MS.gene027150:CDS |
| GCAAGCTGAGGAAGAAGTCA+AGG | 0.547712 | 3.4:+47850702 | MS.gene027150:CDS |
| TGAAGAGCAATTATATTGAT+GGG | 0.547887 | 3.4:+47853221 | MS.gene027150:CDS |
| GAGCAATTATATTGATGGGA+AGG | 0.549182 | 3.4:+47853225 | MS.gene027150:CDS |
| AGGAGTCGCTTCTAAACTTG+TGG | 0.551270 | 3.4:+47852866 | MS.gene027150:CDS |
| ATCAGAGTTGGTCTTCGGGA+CGG | 0.551912 | 3.4:-47853155 | None:intergenic |
| CGTGCAGGATTCTATAAACC+AGG | 0.552805 | 3.4:+47855962 | MS.gene027150:CDS |
| CTCCTTCTCTCCGAAAGATA+GGG | 0.554373 | 3.4:-47852848 | None:intergenic |
| AAGATATGTGTCCCCGGATG+TGG | 0.555259 | 3.4:+47852620 | MS.gene027150:CDS |
| AAACTTTGAGTATCCACGTC+AGG | 0.555864 | 3.4:-47848349 | None:intergenic |
| AACTCAGCTGAAAGTGGCGG+AGG | 0.557051 | 3.4:+47851504 | MS.gene027150:CDS |
| CCGTTCATTACAGACTTCAC+AGG | 0.557711 | 3.4:-47849969 | None:intergenic |
| TTCATGACGAAGAAGCTCAA+GGG | 0.560084 | 3.4:+47848867 | MS.gene027150:CDS |
| CGTTTCTTAAGACCACATCC+GGG | 0.561543 | 3.4:-47852632 | None:intergenic |
| AGGATATGGACGCCACTCCC+GGG | 0.562181 | 3.4:-47851352 | None:intergenic |
| GGCGGAGGAGGCTTTGTCAG+GGG | 0.562222 | 3.4:+47851519 | MS.gene027150:CDS |
| GTATTCCTCTGGCTAATCTA+GGG | 0.563962 | 3.4:-47857777 | None:intergenic |
| GATCGCCAAGACGCTATCCT+CGG | 0.566309 | 3.4:+47852756 | MS.gene027150:CDS |
| GCAAGGTGCTGCTTACGTCT+CGG | 0.567387 | 3.4:+47848734 | MS.gene027150:intron |
| AAAGATGGGTTATGAGGAGG+TGG | 0.568025 | 3.4:+47848800 | MS.gene027150:CDS |
| TCTACAACGATGGTGTCCCC+GGG | 0.568555 | 3.4:+47848265 | None:intergenic |
| GCAAGCTGTGGAAGAAGTCG+AGG | 0.568949 | 3.4:+47854505 | MS.gene027150:CDS |
| TTGCGGCCTAAGGTACATCA+TGG | 0.569258 | 3.4:-47857704 | None:intergenic |
| TGGCGGAGGAGGCTTTGTCA+GGG | 0.569871 | 3.4:+47851518 | MS.gene027150:CDS |
| GTTGCAGCAGATGATCTCCT+AGG | 0.569948 | 3.4:+47848394 | MS.gene027150:CDS |
| AGATTCATCAAAGGATTCCT+CGG | 0.570696 | 3.4:-47850800 | None:intergenic |
| AAACTTTCAAGATCCACGTC+AGG | 0.572396 | 3.4:-47852699 | None:intergenic |
| ATCATGACGAAGAAGTTCGA+GGG | 0.572521 | 3.4:+47853262 | MS.gene027150:CDS |
| CAGAGGAATACAGATGATCA+AGG | 0.573722 | 3.4:+47857789 | MS.gene027150:CDS |
| CGAAAAGATGGGGTATGAGA+AGG | 0.577206 | 3.4:+47853192 | MS.gene027150:CDS |
| GATGGGTTATGAGGAGGTGG+CGG | 0.578268 | 3.4:+47848803 | MS.gene027150:CDS |
| CTTGATTTAGCTCGGGAGAG+GGG | 0.578698 | 3.4:+47855757 | MS.gene027150:CDS |
| GTTGGTGAACTGAAGCGTAC+TGG | 0.578758 | 3.4:+47848445 | MS.gene027150:CDS |
| ACATCCTCGGTGGACGTTTG+GGG | 0.578826 | 3.4:-47857826 | None:intergenic |
| GAAGAGTAATCCATCAGAGT+TGG | 0.583113 | 3.4:-47853167 | None:intergenic |
| AAAGATAGGGCCAACGAAGA+GGG | 0.584375 | 3.4:-47848485 | None:intergenic |
| ATGCGTTCTGCGGAGACCGA+AGG | 0.587376 | 3.4:+47848897 | MS.gene027150:CDS |
| GAAGAGTAATGCGTCAGTGT+TGG | 0.587807 | 3.4:-47848772 | None:intergenic |
| GTACCTTAGGCCGCAAATAG+GGG | 0.589414 | 3.4:+47857711 | MS.gene027150:CDS |
| ATCTATGAAGTCTGGAGCGG+AGG | 0.589831 | 3.4:+47852575 | MS.gene027150:intron |
| TACTCTTCTTCGAAAAGATG+GGG | 0.590062 | 3.4:+47853182 | MS.gene027150:CDS |
| CATCATGACGAAGAAGTTCG+AGG | 0.591689 | 3.4:+47853261 | MS.gene027150:CDS |
| ATGCATTATGCGGAGACTGA+AGG | 0.591978 | 3.4:+47853292 | MS.gene027150:CDS |
| CGTAACCGAGGATAGCGTCT+TGG | 0.592411 | 3.4:-47852761 | None:intergenic |
| GCTTTGCACGTGTTTACTTG+TGG | 0.594793 | 3.4:-47857869 | None:intergenic |
| TGAAGAGCAATAGTATTGAT+GGG | 0.595651 | 3.4:+47848826 | MS.gene027150:CDS |
| GACGGTCCATGATGTACCTT+AGG | 0.595863 | 3.4:+47857698 | MS.gene027150:CDS |
| TGTCCGACTTCAAGAAGCCC+GGG | 0.597660 | 3.4:+47851335 | MS.gene027150:CDS |
| TGTTGAGTTTGCAACTGCAG+AGG | 0.598820 | 3.4:+47855572 | MS.gene027150:CDS |
| AAGGTTCTGGCGGAACGCAA+AGG | 0.601781 | 3.4:+47857619 | MS.gene027150:CDS |
| GAAAGATAGGGCCAACGAAG+AGG | 0.603202 | 3.4:-47848486 | None:intergenic |
| AAAGAAGCAAAAGGTAGATG+AGG | 0.604240 | 3.4:+47854538 | MS.gene027150:CDS |
| AGTAACAACTCAGCTGAAAG+TGG | 0.605182 | 3.4:+47851498 | MS.gene027150:intron |
| CACATCCTCAGATTCATGAA+AGG | 0.607833 | 3.4:-47854612 | None:intergenic |
| ACTCCCGGGCTTCTTGAAGT+CGG | 0.608851 | 3.4:-47851338 | None:intergenic |
| TTATGCAAGATATGTGTCCC+CGG | 0.610053 | 3.4:+47852614 | MS.gene027150:CDS |
| GAACCGAAGAGTAATGGTTG+TGG | 0.611924 | 3.4:+47852801 | MS.gene027150:CDS |
| ATTCATGACGAAGAAGCTCA+AGG | 0.613048 | 3.4:+47848866 | MS.gene027150:CDS |
| GGATTATCTCTTACCTGACG+TGG | 0.613896 | 3.4:+47852686 | MS.gene027150:CDS |
| TTTACTTTGATATAGGCAAG+AGG | 0.615439 | 3.4:+47850680 | MS.gene027150:intron |
| TTGCATCCTGAGGTGTACCA+TGG | 0.615559 | 3.4:-47857564 | None:intergenic |
| CACGTCCTCAGATTCATCAA+AGG | 0.616561 | 3.4:-47850809 | None:intergenic |
| TTGACTTTGATATAGGCAAG+AGG | 0.619505 | 3.4:+47854483 | MS.gene027150:intron |
| GGAATCCTTTGATGAATCTG+AGG | 0.620629 | 3.4:+47850804 | MS.gene027150:CDS |
| CATGCCTGAATCAACAATGA+CGG | 0.621646 | 3.4:+47857654 | MS.gene027150:CDS |
| GGTAACCGAGGATAATGCCT+AGG | 0.623647 | 3.4:-47848411 | None:intergenic |
| GGAATCTTCTGATGAAGATG+AGG | 0.626607 | 3.4:+47852436 | MS.gene027150:CDS |
| GTCGTTGAACCGAAGAGTAA+TGG | 0.627136 | 3.4:+47852795 | MS.gene027150:CDS |
| TGAATCTTCTGATGAAGATG+AGG | 0.627880 | 3.4:+47849433 | MS.gene027150:CDS |
| CGAAAAGATGGGTTATGAGG+AGG | 0.628296 | 3.4:+47848797 | MS.gene027150:CDS |
| GGAATCCTTTCATGAATCTG+AGG | 0.630260 | 3.4:+47854607 | MS.gene027150:CDS |
| CGTCTCTTACGACCACACCC+GGG | 0.631420 | 3.4:-47848282 | None:intergenic |
| ACCAAGGTCCAGGATATCCA+TGG | 0.631502 | 3.4:+47857547 | MS.gene027150:intron |
| AAAGATGGGGTATGAGAAGG+AGG | 0.631960 | 3.4:+47853195 | MS.gene027150:CDS |
| GGTAGTCGAAATTAGTTCCG+AGG | 0.633179 | 3.4:+47850783 | MS.gene027150:CDS |
| AAAGCGCTGAAAAGGACTTG+AGG | 0.634334 | 3.4:+47852889 | MS.gene027150:CDS |
| TCATGACGAAGAAGCTCAAG+GGG | 0.636037 | 3.4:+47848868 | MS.gene027150:CDS |
| CCTGTGAAGTCTGTAATGAA+CGG | 0.636917 | 3.4:+47849969 | MS.gene027150:CDS |
| TTCATCTATGAAGTCTGGAG+CGG | 0.637375 | 3.4:+47852572 | MS.gene027150:intron |
| GGTCTGTCCTGATGAAGATG+AGG | 0.638915 | 3.4:+47849216 | MS.gene027150:CDS |
| TTGCACGTGTTTACTTGTGG+AGG | 0.639379 | 3.4:-47857866 | None:intergenic |
| TTCTCAAACAAGATTCAAGA+CGG | 0.642325 | 3.4:+47857680 | MS.gene027150:CDS |
| TGTTGAGTTTGAAACTGCAG+AGG | 0.644896 | 3.4:+47851167 | MS.gene027150:CDS |
| GATCTCCTAGGCATTATCCT+CGG | 0.649171 | 3.4:+47848406 | MS.gene027150:CDS |
| TATAGGCAAGAGGCAAGCTG+TGG | 0.650683 | 3.4:+47854493 | MS.gene027150:intron |
| GTCTCTTACGACCACACCCG+GGG | 0.653034 | 3.4:-47848281 | None:intergenic |
| TGTAATAACTTCACTTCACG+AGG | 0.654474 | 3.4:-47849187 | None:intergenic |
| AGTTCGAACTAAGGTTCTGG+CGG | 0.655667 | 3.4:+47857609 | MS.gene027150:CDS |
| TGAATCTTCTGATGAAGATG+AGG | 0.655782 | 3.4:+47854827 | MS.gene027150:CDS |
| GGGGAAGCAGATGCATTATG+CGG | 0.657065 | 3.4:+47853282 | MS.gene027150:CDS |
| TCAACATGTGCATAGCCTAG+TGG | 0.659004 | 3.4:-47851150 | None:intergenic |
| CATCAAAGACAAGGTAACCG+AGG | 0.661196 | 3.4:-47848423 | None:intergenic |
| GGGGAAGCAGATGCGTTCTG+CGG | 0.668612 | 3.4:+47848887 | MS.gene027150:CDS |
| ATAATAAGAGGATATCCTTG+AGG | 0.672467 | 3.4:-47851372 | None:intergenic |
| GAGTGCACCTGAAAAGAAAG+CGG | 0.672655 | 3.4:+47852388 | MS.gene027150:CDS |
| AACAACTCAGCTGAAAGTGG+CGG | 0.672747 | 3.4:+47851501 | MS.gene027150:CDS |
| GAGTGGCGTCCATATCCTCA+AGG | 0.678657 | 3.4:+47851357 | MS.gene027150:CDS |
| TCTCCCGAGCTAAATCAAGT+CGG | 0.679997 | 3.4:-47855753 | None:intergenic |
| ATAGGCACTTGATAAGAATG+GGG | 0.680446 | 3.4:+47851293 | MS.gene027150:intron |
| TATAGGCAAGAGGCAAGCTG+AGG | 0.681134 | 3.4:+47850690 | MS.gene027150:intron |
| GTTCACCAACATCAAAGACA+AGG | 0.681557 | 3.4:-47848432 | None:intergenic |
| TATAAACCAGGCCTATACCA+AGG | 0.685036 | 3.4:+47855974 | MS.gene027150:CDS |
| GAATGATGTCTTTCCTGACG+TGG | 0.685326 | 3.4:+47848336 | MS.gene027150:CDS |
| GTTTCTTAAGACCACATCCG+GGG | 0.688273 | 3.4:-47852631 | None:intergenic |
| AAAGAAGCAAAAGGTAGACG+AGG | 0.690556 | 3.4:+47850735 | MS.gene027150:CDS |
| TCATGACGAAGAAGTTCGAG+GGG | 0.691102 | 3.4:+47853263 | MS.gene027150:CDS |
| ATACAACATTAGAAGAAACA+GGG | 0.691600 | 3.4:-47855908 | None:intergenic |
| CATCAAAGATGACGTAACCG+AGG | 0.714623 | 3.4:-47852773 | None:intergenic |
| GCTTGCCCTAGATTAGCCAG+AGG | 0.726595 | 3.4:+47857772 | MS.gene027150:CDS |
| CCCGCCCCAAACGTCCACCG+AGG | 0.730115 | 3.4:+47857822 | MS.gene027150:CDS |
| GGTCGAACTAAGGTTCAAGG+TGG | 0.741730 | 3.4:+47857750 | MS.gene027150:CDS |
| TAAGATTCTTCAAGTCATCG+CGG | 0.762237 | 3.4:+47852731 | MS.gene027150:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 47848274 | 47857897 | 47848274 | ID=MS.gene027150 |
| chr3.4 | mRNA | 47848274 | 47857897 | 47848274 | ID=MS.gene027150.t1;Parent=MS.gene027150 |
| chr3.4 | exon | 47848274 | 47848564 | 47848274 | ID=MS.gene027150.t1.exon1;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47848274 | 47848564 | 47848274 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| chr3.4 | exon | 47848735 | 47848918 | 47848735 | ID=MS.gene027150.t1.exon2;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47848735 | 47848918 | 47848735 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| chr3.4 | exon | 47849170 | 47849237 | 47849170 | ID=MS.gene027150.t1.exon3;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47849170 | 47849237 | 47849170 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| chr3.4 | exon | 47849353 | 47849454 | 47849353 | ID=MS.gene027150.t1.exon4;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47849353 | 47849454 | 47849353 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| chr3.4 | exon | 47849942 | 47849990 | 47849942 | ID=MS.gene027150.t1.exon5;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47849942 | 47849990 | 47849942 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| chr3.4 | exon | 47850695 | 47850831 | 47850695 | ID=MS.gene027150.t1.exon6;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47850695 | 47850831 | 47850695 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| chr3.4 | exon | 47850959 | 47851005 | 47850959 | ID=MS.gene027150.t1.exon7;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47850959 | 47851005 | 47850959 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| chr3.4 | exon | 47851083 | 47851200 | 47851083 | ID=MS.gene027150.t1.exon8;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47851083 | 47851200 | 47851083 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| chr3.4 | exon | 47851297 | 47851400 | 47851297 | ID=MS.gene027150.t1.exon9;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47851297 | 47851400 | 47851297 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| chr3.4 | exon | 47851500 | 47851569 | 47851500 | ID=MS.gene027150.t1.exon10;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47851500 | 47851569 | 47851500 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| chr3.4 | exon | 47852353 | 47852457 | 47852353 | ID=MS.gene027150.t1.exon11;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47852353 | 47852457 | 47852353 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| chr3.4 | exon | 47852585 | 47852917 | 47852585 | ID=MS.gene027150.t1.exon12;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47852585 | 47852917 | 47852585 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| chr3.4 | exon | 47853130 | 47853313 | 47853130 | ID=MS.gene027150.t1.exon13;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47853130 | 47853313 | 47853130 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| chr3.4 | exon | 47854498 | 47854634 | 47854498 | ID=MS.gene027150.t1.exon14;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47854498 | 47854634 | 47854498 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| chr3.4 | exon | 47854735 | 47854848 | 47854735 | ID=MS.gene027150.t1.exon15;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47854735 | 47854848 | 47854735 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| chr3.4 | exon | 47855323 | 47855411 | 47855323 | ID=MS.gene027150.t1.exon16;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47855323 | 47855411 | 47855323 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| chr3.4 | exon | 47855488 | 47855605 | 47855488 | ID=MS.gene027150.t1.exon17;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47855488 | 47855605 | 47855488 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| chr3.4 | exon | 47855712 | 47855803 | 47855712 | ID=MS.gene027150.t1.exon18;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47855712 | 47855803 | 47855712 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| chr3.4 | exon | 47855890 | 47855995 | 47855890 | ID=MS.gene027150.t1.exon19;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47855890 | 47855995 | 47855890 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| chr3.4 | exon | 47857553 | 47857897 | 47857553 | ID=MS.gene027150.t1.exon20;Parent=MS.gene027150.t1 |
| chr3.4 | CDS | 47857553 | 47857897 | 47857553 | ID=cds.MS.gene027150.t1;Parent=MS.gene027150.t1 |
| Gene Sequence |
| Protein sequence |
