Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041376.t1 | XP_003609480.1 | 99.1 | 567 | 3 | 1 | 1 | 565 | 1 | 567 | 8.00E-257 | 896.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041376.t1 | Q52T38 | 74.5 | 556 | 138 | 2 | 1 | 555 | 1 | 553 | 2.9e-191 | 669.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041376.t1 | G7JGL7 | 99.1 | 567 | 3 | 1 | 1 | 565 | 1 | 567 | 5.8e-257 | 896.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene024088 | MS.gene041376 | PPI |
| MS.gene39295 | MS.gene041376 | PPI |
| MS.gene064692 | MS.gene041376 | PPI |
| MS.gene037158 | MS.gene041376 | PPI |
| MS.gene02446 | MS.gene041376 | PPI |
| MS.gene42314 | MS.gene041376 | PPI |
| MS.gene033532 | MS.gene041376 | PPI |
| MS.gene30580 | MS.gene041376 | PPI |
| MS.gene34516 | MS.gene041376 | PPI |
| MS.gene010517 | MS.gene041376 | PPI |
| MS.gene024089 | MS.gene041376 | PPI |
| MS.gene64467 | MS.gene041376 | PPI |
| MS.gene010536 | MS.gene041376 | PPI |
| MS.gene027143 | MS.gene041376 | PPI |
| MS.gene30139 | MS.gene041376 | PPI |
| MS.gene82682 | MS.gene041376 | PPI |
| MS.gene59380 | MS.gene041376 | PPI |
| MS.gene43653 | MS.gene041376 | PPI |
| MS.gene82663 | MS.gene041376 | PPI |
| MS.gene020216 | MS.gene041376 | PPI |
| MS.gene062547 | MS.gene041376 | PPI |
| MS.gene027150 | MS.gene041376 | PPI |
| MS.gene047793 | MS.gene041376 | PPI |
| MS.gene057397 | MS.gene041376 | PPI |
| MS.gene64679 | MS.gene041376 | PPI |
| MS.gene02437 | MS.gene041376 | PPI |
| MS.gene022751 | MS.gene041376 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041376.t1 | MTR_4g116160 | 99.377 | 642 | 2 | 1 | 1 | 640 | 1 | 642 | 0.0 | 1328 |
| MS.gene041376.t1 | MTR_4g116160 | 96.647 | 507 | 13 | 2 | 1 | 505 | 1 | 505 | 0.0 | 1018 |
| MS.gene041376.t1 | MTR_4g116160 | 99.785 | 466 | 1 | 0 | 175 | 640 | 4 | 469 | 0.0 | 973 |
| MS.gene041376.t1 | MTR_1g112830 | 66.143 | 573 | 145 | 9 | 1 | 571 | 106 | 631 | 0.0 | 737 |
| MS.gene041376.t1 | MTR_4g092760 | 52.788 | 538 | 240 | 2 | 2 | 538 | 3 | 527 | 0.0 | 559 |
| MS.gene041376.t1 | MTR_4g092760 | 54.005 | 437 | 200 | 1 | 102 | 538 | 3 | 438 | 1.85e-164 | 478 |
| MS.gene041376.t1 | MTR_1g112820 | 50.000 | 540 | 111 | 9 | 1 | 540 | 99 | 479 | 4.69e-158 | 479 |
| MS.gene041376.t1 | MTR_6g033315 | 27.089 | 347 | 198 | 12 | 266 | 580 | 39 | 362 | 1.64e-20 | 95.1 |
| MS.gene041376.t1 | MTR_7g088360 | 28.879 | 232 | 117 | 9 | 319 | 526 | 92 | 299 | 4.07e-20 | 93.6 |
| MS.gene041376.t1 | MTR_3g098840 | 27.490 | 251 | 151 | 8 | 327 | 558 | 93 | 331 | 1.23e-18 | 89.4 |
| MS.gene041376.t1 | MTR_7g088350 | 31.441 | 229 | 123 | 9 | 319 | 531 | 93 | 303 | 2.76e-18 | 88.6 |
| MS.gene041376.t1 | MTR_7g114080 | 41.176 | 102 | 49 | 2 | 323 | 424 | 120 | 210 | 7.27e-18 | 85.5 |
| MS.gene041376.t1 | MTR_1g094610 | 27.240 | 279 | 152 | 9 | 269 | 531 | 3 | 246 | 1.24e-17 | 84.0 |
| MS.gene041376.t1 | MTR_7g083480 | 30.769 | 234 | 120 | 6 | 319 | 531 | 84 | 296 | 1.37e-17 | 86.3 |
| MS.gene041376.t1 | MTR_7g114080 | 41.176 | 102 | 49 | 2 | 323 | 424 | 120 | 210 | 1.93e-17 | 85.1 |
| MS.gene041376.t1 | MTR_7g114080 | 41.176 | 102 | 49 | 2 | 323 | 424 | 120 | 210 | 1.95e-17 | 85.1 |
| MS.gene041376.t1 | MTR_5g023230 | 26.437 | 261 | 118 | 8 | 320 | 530 | 57 | 293 | 3.25e-17 | 83.6 |
| MS.gene041376.t1 | MTR_5g023230 | 26.437 | 261 | 118 | 8 | 320 | 530 | 77 | 313 | 3.81e-17 | 84.0 |
| MS.gene041376.t1 | MTR_1g098390 | 36.207 | 116 | 63 | 2 | 319 | 423 | 70 | 185 | 5.23e-17 | 84.3 |
| MS.gene041376.t1 | MTR_7g114080 | 41.176 | 102 | 49 | 2 | 323 | 424 | 120 | 210 | 5.90e-17 | 82.8 |
| MS.gene041376.t1 | MTR_7g115280 | 31.330 | 233 | 155 | 3 | 23 | 255 | 44 | 271 | 6.96e-17 | 84.3 |
| MS.gene041376.t1 | MTR_6g038020 | 34.454 | 119 | 64 | 3 | 319 | 423 | 68 | 186 | 1.65e-16 | 82.8 |
| MS.gene041376.t1 | MTR_6g038020 | 34.454 | 119 | 64 | 3 | 319 | 423 | 71 | 189 | 2.05e-16 | 82.4 |
| MS.gene041376.t1 | MTR_5g023230 | 31.847 | 157 | 60 | 5 | 320 | 434 | 77 | 228 | 1.76e-15 | 77.0 |
| MS.gene041376.t1 | MTR_5g043640 | 33.121 | 157 | 99 | 3 | 102 | 255 | 55 | 208 | 3.74e-14 | 73.2 |
| MS.gene041376.t1 | MTR_5g043640 | 31.765 | 170 | 111 | 3 | 94 | 263 | 83 | 247 | 7.18e-13 | 69.3 |
| MS.gene041376.t1 | MTR_5g043640 | 28.902 | 173 | 120 | 2 | 35 | 205 | 55 | 226 | 1.38e-12 | 68.6 |
| MS.gene041376.t1 | MTR_4g021795 | 26.000 | 250 | 163 | 7 | 291 | 531 | 23 | 259 | 5.27e-14 | 73.6 |
| MS.gene041376.t1 | MTR_1g085980 | 40.909 | 88 | 46 | 2 | 351 | 438 | 139 | 220 | 7.59e-14 | 74.7 |
| MS.gene041376.t1 | MTR_0020s0130 | 29.412 | 238 | 120 | 7 | 33 | 224 | 9 | 244 | 2.81e-13 | 73.2 |
| MS.gene041376.t1 | MTR_1g089180 | 29.825 | 171 | 105 | 4 | 85 | 255 | 270 | 425 | 3.14e-13 | 72.8 |
| MS.gene041376.t1 | MTR_4g023760 | 27.090 | 299 | 169 | 15 | 279 | 560 | 21 | 287 | 3.16e-13 | 71.2 |
| MS.gene041376.t1 | MTR_6g027840 | 31.548 | 168 | 107 | 3 | 61 | 224 | 170 | 333 | 3.31e-13 | 72.8 |
| MS.gene041376.t1 | MTR_3g115950 | 32.192 | 146 | 74 | 5 | 375 | 504 | 75 | 211 | 6.22e-12 | 68.9 |
| MS.gene041376.t1 | MTR_3g115950 | 31.884 | 138 | 85 | 3 | 375 | 504 | 149 | 285 | 8.84e-12 | 68.6 |
| MS.gene041376.t1 | MTR_4g082560 | 47.368 | 57 | 27 | 1 | 376 | 432 | 181 | 234 | 1.55e-11 | 67.8 |
| MS.gene041376.t1 | MTR_4g062100 | 31.606 | 193 | 123 | 6 | 38 | 224 | 109 | 298 | 2.65e-11 | 67.0 |
| MS.gene041376.t1 | MTR_1g115575 | 30.319 | 188 | 127 | 4 | 38 | 221 | 135 | 322 | 5.08e-11 | 66.2 |
| MS.gene041376.t1 | MTR_5g042870 | 53.333 | 45 | 21 | 0 | 376 | 420 | 181 | 225 | 5.10e-11 | 66.2 |
| MS.gene041376.t1 | MTR_4g076120 | 32.877 | 73 | 49 | 0 | 370 | 442 | 103 | 175 | 6.98e-11 | 65.9 |
| MS.gene041376.t1 | MTR_4g076120 | 32.877 | 73 | 49 | 0 | 370 | 442 | 177 | 249 | 9.21e-11 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041376.t1 | AT5G20350 | 70.561 | 642 | 164 | 5 | 1 | 640 | 1 | 619 | 0.0 | 915 |
| MS.gene041376.t1 | AT2G14255 | 50.000 | 544 | 260 | 2 | 2 | 545 | 3 | 534 | 0.0 | 557 |
| MS.gene041376.t1 | AT5G50020 | 30.104 | 289 | 152 | 12 | 277 | 530 | 20 | 293 | 2.62e-23 | 103 |
| MS.gene041376.t1 | AT5G50020 | 30.104 | 289 | 152 | 12 | 277 | 530 | 50 | 323 | 3.90e-23 | 103 |
| MS.gene041376.t1 | AT3G26935 | 29.412 | 221 | 130 | 6 | 319 | 526 | 83 | 290 | 5.95e-20 | 93.6 |
| MS.gene041376.t1 | AT4G24630 | 29.741 | 232 | 137 | 6 | 319 | 530 | 69 | 294 | 8.29e-20 | 92.8 |
| MS.gene041376.t1 | AT5G41060 | 29.075 | 227 | 133 | 6 | 319 | 532 | 16 | 227 | 1.75e-19 | 90.9 |
| MS.gene041376.t1 | AT5G41060 | 29.075 | 227 | 133 | 6 | 319 | 532 | 16 | 227 | 1.75e-19 | 90.9 |
| MS.gene041376.t1 | AT5G41060 | 28.261 | 276 | 159 | 8 | 272 | 533 | 30 | 280 | 4.22e-19 | 90.5 |
| MS.gene041376.t1 | AT5G41060 | 28.261 | 276 | 159 | 8 | 272 | 533 | 45 | 295 | 5.11e-19 | 90.5 |
| MS.gene041376.t1 | AT5G05070 | 26.408 | 284 | 151 | 11 | 319 | 577 | 100 | 350 | 8.03e-19 | 89.7 |
| MS.gene041376.t1 | AT5G50020 | 28.720 | 289 | 149 | 13 | 277 | 530 | 20 | 286 | 1.89e-18 | 88.6 |
| MS.gene041376.t1 | AT3G48760 | 29.412 | 204 | 122 | 7 | 334 | 526 | 107 | 299 | 2.33e-18 | 89.0 |
| MS.gene041376.t1 | AT3G04970 | 38.462 | 104 | 53 | 2 | 321 | 424 | 31 | 123 | 1.13e-16 | 82.0 |
| MS.gene041376.t1 | AT3G56930 | 25.746 | 268 | 170 | 9 | 303 | 555 | 63 | 316 | 1.15e-16 | 83.6 |
| MS.gene041376.t1 | AT3G04970 | 38.462 | 104 | 53 | 2 | 321 | 424 | 118 | 210 | 1.45e-16 | 81.6 |
| MS.gene041376.t1 | AT3G04970 | 38.462 | 104 | 53 | 2 | 321 | 424 | 118 | 210 | 4.02e-16 | 81.3 |
| MS.gene041376.t1 | AT3G04710 | 30.263 | 228 | 154 | 3 | 28 | 255 | 49 | 271 | 1.19e-15 | 80.5 |
| MS.gene041376.t1 | AT3G04710 | 30.263 | 228 | 154 | 3 | 28 | 255 | 273 | 495 | 1.25e-15 | 81.3 |
| MS.gene041376.t1 | AT2G03430 | 33.939 | 165 | 103 | 3 | 94 | 255 | 40 | 201 | 3.37e-15 | 76.3 |
| MS.gene041376.t1 | AT2G03430 | 30.636 | 173 | 117 | 2 | 35 | 205 | 48 | 219 | 3.88e-14 | 73.2 |
| MS.gene041376.t1 | AT3G56920 | 26.506 | 249 | 148 | 7 | 319 | 550 | 73 | 303 | 4.28e-15 | 77.4 |
| MS.gene041376.t1 | AT2G40990 | 30.000 | 170 | 83 | 4 | 374 | 532 | 160 | 304 | 8.50e-15 | 77.4 |
| MS.gene041376.t1 | AT3G04710 | 29.386 | 228 | 155 | 3 | 28 | 255 | 49 | 270 | 1.23e-14 | 77.0 |
| MS.gene041376.t1 | AT3G51390 | 25.301 | 249 | 144 | 6 | 320 | 538 | 73 | 309 | 2.34e-14 | 75.1 |
| MS.gene041376.t1 | AT3G51390 | 30.061 | 163 | 102 | 3 | 376 | 538 | 164 | 314 | 1.63e-13 | 72.8 |
| MS.gene041376.t1 | AT5G07270 | 28.916 | 249 | 137 | 8 | 33 | 243 | 9 | 255 | 2.11e-13 | 73.6 |
| MS.gene041376.t1 | AT3G56930 | 25.000 | 268 | 136 | 8 | 303 | 555 | 63 | 280 | 3.26e-13 | 72.8 |
| MS.gene041376.t1 | AT3G02850 | 35.632 | 174 | 108 | 3 | 40 | 213 | 554 | 723 | 4.88e-13 | 72.8 |
| MS.gene041376.t1 | AT5G04270 | 29.878 | 164 | 99 | 4 | 373 | 531 | 91 | 243 | 5.28e-13 | 70.1 |
| MS.gene041376.t1 | AT3G02850 | 35.632 | 174 | 108 | 3 | 40 | 213 | 554 | 723 | 5.52e-13 | 72.8 |
| MS.gene041376.t1 | AT3G09320 | 29.500 | 200 | 122 | 8 | 336 | 531 | 66 | 250 | 8.40e-13 | 69.7 |
| MS.gene041376.t1 | AT5G37500 | 32.240 | 183 | 120 | 3 | 38 | 220 | 535 | 713 | 3.24e-12 | 70.5 |
| MS.gene041376.t1 | AT5G66055 | 31.293 | 147 | 95 | 2 | 64 | 205 | 251 | 396 | 4.21e-12 | 69.3 |
| MS.gene041376.t1 | AT1G69420 | 29.078 | 141 | 83 | 3 | 376 | 504 | 165 | 300 | 6.50e-12 | 68.9 |
| MS.gene041376.t1 | AT1G69420 | 29.078 | 141 | 83 | 3 | 376 | 504 | 165 | 300 | 6.50e-12 | 68.9 |
| MS.gene041376.t1 | AT5G40160 | 32.867 | 143 | 91 | 2 | 68 | 208 | 152 | 291 | 1.73e-11 | 66.2 |
| MS.gene041376.t1 | AT4G01730 | 42.857 | 63 | 36 | 0 | 376 | 438 | 160 | 222 | 1.80e-11 | 67.4 |
| MS.gene041376.t1 | AT3G60800 | 25.566 | 309 | 169 | 14 | 279 | 560 | 21 | 295 | 4.21e-11 | 65.1 |
| MS.gene041376.t1 | AT4G15080 | 35.294 | 68 | 44 | 0 | 375 | 442 | 175 | 242 | 6.88e-11 | 65.9 |
| MS.gene041376.t1 | AT4G15080 | 35.294 | 68 | 44 | 0 | 375 | 442 | 175 | 242 | 6.88e-11 | 65.9 |
Find 170 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAGGAATGCTGTCATTTC+TGG | 0.069774 | 8.1:+10956642 | None:intergenic |
| CACTTGCTCCTTCTTCGTTT+GGG | 0.171078 | 8.1:-10957257 | MS.gene041376:CDS |
| TATGATTGCAGAAGAATAAA+TGG | 0.200675 | 8.1:-10957446 | MS.gene041376:intron |
| TGCGGTTCGGGGTGCTATTC+AGG | 0.206164 | 8.1:-10963404 | MS.gene041376:CDS |
| GTGTACACTGCCGCGGCTTA+TGG | 0.225283 | 8.1:-10965055 | MS.gene041376:CDS |
| CCACTTGCTCCTTCTTCGTT+TGG | 0.228835 | 8.1:-10957258 | MS.gene041376:CDS |
| TTTACTATGTTATTTCAAAA+TGG | 0.249520 | 8.1:-10963075 | MS.gene041376:CDS |
| CTTGGAAAGATATCTAAATT+AGG | 0.292805 | 8.1:-10959735 | MS.gene041376:CDS |
| CTGCTTTGCTAGCTGGAAAT+TGG | 0.305604 | 8.1:-10958425 | MS.gene041376:CDS |
| GTTTGCTTTGACAGCTGTTC+AGG | 0.306580 | 8.1:-10957154 | MS.gene041376:CDS |
| ATTGCATTGGAGTGCGGTTC+GGG | 0.307377 | 8.1:-10963416 | MS.gene041376:CDS |
| TTCTTGATACCATGATCATA+TGG | 0.313589 | 8.1:+10956943 | None:intergenic |
| TGCATGGTTTGGAGTGTTGC+TGG | 0.319783 | 8.1:-10959474 | MS.gene041376:CDS |
| CTCCTTCTTCGTTTGGGGCA+TGG | 0.319869 | 8.1:-10957251 | MS.gene041376:CDS |
| GTTTCAGCAATGTTGGTTAC+TGG | 0.323373 | 8.1:-10957396 | MS.gene041376:CDS |
| TGTACACTGCCGCGGCTTAT+GGG | 0.329150 | 8.1:-10965054 | MS.gene041376:CDS |
| CATTGCATTGGAGTGCGGTT+CGG | 0.330833 | 8.1:-10963417 | MS.gene041376:CDS |
| GGGCATCTTCCATCTTTATC+AGG | 0.335484 | 8.1:+10963034 | None:intergenic |
| TGTTAACGAGCCTGATGGTT+TGG | 0.339227 | 8.1:-10964987 | MS.gene041376:CDS |
| CGCTGACATGAATGGGTATC+AGG | 0.339731 | 8.1:-10963341 | MS.gene041376:intron |
| CACTAATATCAGTCACTATC+AGG | 0.344409 | 8.1:+10960974 | None:intergenic |
| CCATCTTTATCAGGAGCATC+AGG | 0.357949 | 8.1:+10963043 | None:intergenic |
| GTTAACGAGCCTGATGGTTT+GGG | 0.358664 | 8.1:-10964986 | MS.gene041376:CDS |
| TGCCGCGGCTTATGGGGATT+TGG | 0.378653 | 8.1:-10965047 | MS.gene041376:CDS |
| ACACTCCAAACCATGCAAAA+AGG | 0.379104 | 8.1:+10959480 | None:intergenic |
| GTTAGTACAGGCTGGCAAAA+AGG | 0.379324 | 8.1:-10961001 | MS.gene041376:CDS |
| CCAGCTGATGCTGTCAGCTT+TGG | 0.384030 | 8.1:+10959503 | None:intergenic |
| GACAGCTGTTCAGGCTAGTC+AGG | 0.385671 | 8.1:-10957145 | MS.gene041376:intron |
| ATTCCTGTGGGATTAGGTCT+TGG | 0.385952 | 8.1:-10956628 | MS.gene041376:CDS |
| CTATATTCATTCGGTCATCT+TGG | 0.386891 | 8.1:-10959667 | MS.gene041376:CDS |
| CCTTGCATGTTGCACAAAGT+TGG | 0.402484 | 8.1:+10958400 | None:intergenic |
| ATGTGAGTCTCCATTTGTTA+TGG | 0.402722 | 8.1:+10956810 | None:intergenic |
| GGCTGCTATGCGAGGTAACT+TGG | 0.402917 | 8.1:-10961037 | MS.gene041376:CDS |
| CAATATTACAACAAATGAAA+TGG | 0.406071 | 8.1:-10957019 | MS.gene041376:CDS |
| AATAATCCTGCTTTGCTAGC+TGG | 0.407196 | 8.1:-10958432 | MS.gene041376:CDS |
| CACTCCAATGCAATGCTGTC+TGG | 0.408303 | 8.1:+10963425 | None:intergenic |
| GTTATTTCTCGATGCACATC+GGG | 0.416863 | 8.1:-10961224 | MS.gene041376:CDS |
| ACACATGTTGCTGCTCAATA+TGG | 0.418062 | 8.1:-10963112 | MS.gene041376:CDS |
| CAAGATGACCGAATGAATAT+AGG | 0.418447 | 8.1:+10959668 | None:intergenic |
| ATGATTGCAGAAGAATAAAT+GGG | 0.421823 | 8.1:-10957445 | MS.gene041376:intron |
| TTGGAGTATGTTGAAGAATC+AGG | 0.423345 | 8.1:-10956880 | MS.gene041376:CDS |
| CTTGCATGTTGCACAAAGTT+GGG | 0.426675 | 8.1:+10958401 | None:intergenic |
| CGTTCACTCGAGCACCCTCT+TGG | 0.427039 | 8.1:+10963365 | None:intergenic |
| CATGCCAACGGAAACGGAAA+TGG | 0.429129 | 8.1:-10956775 | MS.gene041376:CDS |
| GACAGCATTCCTGTGGGATT+AGG | 0.429472 | 8.1:-10956634 | MS.gene041376:CDS |
| TATATTCATTCGGTCATCTT+GGG | 0.440290 | 8.1:-10959666 | MS.gene041376:intron |
| AATTTGACCATCATTGTCCT+TGG | 0.444619 | 8.1:-10958184 | MS.gene041376:CDS |
| AAGTACCTTGCCAATGCAAT+TGG | 0.446875 | 8.1:+10958157 | None:intergenic |
| CGGCAGTGTACACGTCGTTC+CGG | 0.447089 | 8.1:+10965065 | None:intergenic |
| TAGGACTTGCTCCTGTACTT+TGG | 0.447769 | 8.1:-10959716 | MS.gene041376:CDS |
| AGGTACCACTCCTCTCCATT+GGG | 0.450107 | 8.1:-10961058 | MS.gene041376:CDS |
| CAATTTCCAGCTAGCAAAGC+AGG | 0.450595 | 8.1:+10958426 | None:intergenic |
| TTTGGAGAAGCTACATAGAT+TGG | 0.450998 | 8.1:-10965029 | MS.gene041376:CDS |
| TCTTGATACCATGATCATAT+GGG | 0.451186 | 8.1:+10956944 | None:intergenic |
| AAGAGCATACCGTCTTTATC+CGG | 0.452114 | 8.1:+10961196 | None:intergenic |
| CAGGTACCACTCCTCTCCAT+TGG | 0.452658 | 8.1:-10961059 | MS.gene041376:CDS |
| GTAAATGCAACAGATCATTC+TGG | 0.455394 | 8.1:-10963448 | MS.gene041376:CDS |
| CTATCAGAAGCAAGCTGAGC+TGG | 0.458177 | 8.1:+10960943 | None:intergenic |
| TCTGATCATGCCAACGGAAA+CGG | 0.458195 | 8.1:-10956781 | MS.gene041376:CDS |
| CAGCTCAGCTTGCTTCTGAT+AGG | 0.462435 | 8.1:-10960942 | MS.gene041376:CDS |
| GCGTAGTAACCCAAACCATC+AGG | 0.462483 | 8.1:+10964977 | None:intergenic |
| ATTTGACCATCATTGTCCTT+GGG | 0.468626 | 8.1:-10958183 | MS.gene041376:CDS |
| GGAGTGTTGCTGGCTACTGT+TGG | 0.469546 | 8.1:-10959464 | MS.gene041376:CDS |
| CATAGATTGGTGGAGATTGA+AGG | 0.470207 | 8.1:-10965016 | MS.gene041376:CDS |
| GTTGCTGGCTACTGTTGGAT+TGG | 0.474417 | 8.1:-10959459 | MS.gene041376:CDS |
| GTGAACGCCGCTGACATGAA+TGG | 0.478931 | 8.1:-10963349 | MS.gene041376:CDS |
| GGCAACTTCGTCGACGGCGG+CGG | 0.479440 | 8.1:+10965090 | None:intergenic |
| ATTGGTCACCTATATTCATT+CGG | 0.479806 | 8.1:-10959676 | MS.gene041376:CDS |
| TGCTGCTCAGTACATTATTG+AGG | 0.484936 | 8.1:-10964921 | MS.gene041376:intron |
| AGGGGCTCAACCATAACAAA+TGG | 0.489828 | 8.1:-10956820 | MS.gene041376:CDS |
| TGAACGCCGCTGACATGAAT+GGG | 0.491997 | 8.1:-10963348 | MS.gene041376:CDS |
| TCTCGAAGTTTCAGCAATGT+TGG | 0.497320 | 8.1:-10957403 | MS.gene041376:CDS |
| TGTTATTTCTCGATGCACAT+CGG | 0.498686 | 8.1:-10961225 | MS.gene041376:CDS |
| ACTTGCTCCTTCTTCGTTTG+GGG | 0.499916 | 8.1:-10957256 | MS.gene041376:CDS |
| GCGGCGGAAGATGATGATGA+TGG | 0.502697 | 8.1:+10965106 | None:intergenic |
| GGTAACTCTGAGGAGGGTCT+AGG | 0.503604 | 8.1:-10956859 | MS.gene041376:CDS |
| CCAAAGCTGACAGCATCAGC+TGG | 0.504906 | 8.1:-10959503 | MS.gene041376:CDS |
| GTGGGATTAGGTCTTGGCCT+CGG | 0.506522 | 8.1:-10956622 | MS.gene041376:CDS |
| CTGATAGTGACTGATATTAG+TGG | 0.507438 | 8.1:-10960973 | MS.gene041376:CDS |
| GGGTTACTACGCTCTTCAGT+GGG | 0.513004 | 8.1:-10964966 | MS.gene041376:CDS |
| TTTGACTCTAACAGCCGCCT+TGG | 0.515308 | 8.1:-10959753 | MS.gene041376:CDS |
| AAAATTATGCACCAAAGTAC+AGG | 0.516618 | 8.1:+10959705 | None:intergenic |
| AGTGTACACGTCGTTCCGGC+TGG | 0.517258 | 8.1:+10965069 | None:intergenic |
| CAATGGGTACAATGAAGACT+TGG | 0.518685 | 8.1:-10956899 | MS.gene041376:CDS |
| CGACGAGGATGAAACAGATC+TGG | 0.519824 | 8.1:+10956591 | None:intergenic |
| GGGGCATGGATTAATTATGC+TGG | 0.521153 | 8.1:-10957237 | MS.gene041376:CDS |
| ATGGCCATTTCCGTTTCCGT+TGG | 0.522929 | 8.1:+10956771 | None:intergenic |
| TATAGCTACCTCAGAGGTCC+AGG | 0.523785 | 8.1:-10956982 | MS.gene041376:CDS |
| ATGGAAGATGCCCCTTGCAT+TGG | 0.526333 | 8.1:-10963024 | MS.gene041376:intron |
| CCTTGGGTATCCAATTGCAT+TGG | 0.528779 | 8.1:-10958167 | MS.gene041376:CDS |
| ATCCATGCCCCAAACGAAGA+AGG | 0.528880 | 8.1:+10957249 | None:intergenic |
| CTCCTCGACGACCTCGATCT+CGG | 0.529421 | 8.1:+10965159 | None:intergenic |
| CTGGCCAGACAGCATTGCAT+TGG | 0.529464 | 8.1:-10963429 | MS.gene041376:CDS |
| CCTGATGCTCCTGATAAAGA+TGG | 0.529559 | 8.1:-10963043 | MS.gene041376:CDS |
| CCGGCTGGCAACTTCGTCGA+CGG | 0.531919 | 8.1:+10965084 | None:intergenic |
| CGGCGGAAGATGATGATGAT+GGG | 0.534788 | 8.1:+10965107 | None:intergenic |
| CCGTTCGTTCTGATATAACC+TGG | 0.535275 | 8.1:+10958686 | None:intergenic |
| TTCAGAAACCCATATGATCA+TGG | 0.541446 | 8.1:-10956952 | MS.gene041376:CDS |
| TAGGAATGATGCACATGGCA+AGG | 0.544586 | 8.1:-10956840 | MS.gene041376:CDS |
| GTACACTGCCGCGGCTTATG+GGG | 0.547067 | 8.1:-10965053 | MS.gene041376:CDS |
| AGGAATGATGCACATGGCAA+GGG | 0.547293 | 8.1:-10956839 | MS.gene041376:CDS |
| GACTTCAATTACCAATGCAA+GGG | 0.547760 | 8.1:+10963013 | None:intergenic |
| GGCCTGTACCGTGTTAGTAC+AGG | 0.548851 | 8.1:-10961013 | MS.gene041376:CDS |
| AGCCTGTACTAACACGGTAC+AGG | 0.551233 | 8.1:+10961011 | None:intergenic |
| TGACTTCAATTACCAATGCA+AGG | 0.553922 | 8.1:+10963012 | None:intergenic |
| TCAGCAATGTTGGTTACTGG+TGG | 0.555254 | 8.1:-10957393 | MS.gene041376:CDS |
| CAGGTTATATCAGAACGAAC+GGG | 0.557025 | 8.1:-10958685 | MS.gene041376:CDS |
| TGGATACCCAAGGACAATGA+TGG | 0.558145 | 8.1:+10958177 | None:intergenic |
| TGGGTTACTACGCTCTTCAG+TGG | 0.559516 | 8.1:-10964967 | MS.gene041376:CDS |
| TGCTATGCGAGGTAACTTGG+AGG | 0.560852 | 8.1:-10961034 | MS.gene041376:CDS |
| GGAGAAGCTACATAGATTGG+TGG | 0.562705 | 8.1:-10965026 | MS.gene041376:CDS |
| AAGCAAACACTCCGAAGAAG+AGG | 0.562824 | 8.1:+10957169 | None:intergenic |
| TGGCCATGTTGTTATTAATG+TGG | 0.564216 | 8.1:-10956755 | MS.gene041376:CDS |
| AAGCAAGCTGAGCTGGCGTA+AGG | 0.564597 | 8.1:+10960950 | None:intergenic |
| CTAATTTAGATATCTTTCCA+AGG | 0.565680 | 8.1:+10959736 | None:intergenic |
| CTCCATTGGGCTGCTATGCG+AGG | 0.569685 | 8.1:-10961045 | MS.gene041376:CDS |
| TGTCTTGTTAACGAGCCTGA+TGG | 0.570112 | 8.1:-10964992 | MS.gene041376:CDS |
| CCAGGTTATATCAGAACGAA+CGG | 0.570485 | 8.1:-10958686 | MS.gene041376:CDS |
| CCGTCGACGAAGTTGCCAGC+CGG | 0.572316 | 8.1:-10965084 | MS.gene041376:CDS |
| CCAACTTTGTGCAACATGCA+AGG | 0.575688 | 8.1:-10958400 | MS.gene041376:intron |
| GATTAGGTCTTGGCCTCGGA+CGG | 0.578419 | 8.1:-10956618 | MS.gene041376:CDS |
| TGCTGCTCAATATGGTCAGA+CGG | 0.579560 | 8.1:-10963104 | MS.gene041376:CDS |
| GGTATCCAATTGCATTGGCA+AGG | 0.582714 | 8.1:-10958162 | MS.gene041376:intron |
| AGCTATGTCTTCCGAGATCG+AGG | 0.585048 | 8.1:-10965170 | None:intergenic |
| ACACATCATGGTCATAGTAA+TGG | 0.588135 | 8.1:-10956709 | MS.gene041376:CDS |
| CATCTTTATCAGGAGCATCA+GGG | 0.591415 | 8.1:+10963044 | None:intergenic |
| GGCGGAAGATGATGATGATG+GGG | 0.593138 | 8.1:+10965108 | None:intergenic |
| CGCATAGCAGCCCAATGGAG+AGG | 0.594892 | 8.1:+10961048 | None:intergenic |
| GACAGCATTGCATTGGAGTG+CGG | 0.597347 | 8.1:-10963422 | MS.gene041376:CDS |
| TTCTACATGTGATCGTTGTG+TGG | 0.606564 | 8.1:-10958210 | MS.gene041376:CDS |
| CCAAACGAAGAAGGAGCAAG+TGG | 0.608637 | 8.1:+10957258 | None:intergenic |
| CAGATCTGGTGTTCCGTCCG+AGG | 0.610726 | 8.1:+10956605 | None:intergenic |
| CAGAAATGACAGCATTCCTG+TGG | 0.611047 | 8.1:-10956641 | MS.gene041376:CDS |
| TTGCATTGGAGTGCGGTTCG+GGG | 0.621732 | 8.1:-10963415 | MS.gene041376:CDS |
| AGGCCAAGACCTAATCCCAC+AGG | 0.622435 | 8.1:+10956625 | None:intergenic |
| CTCCAAATCCCCATAAGCCG+CGG | 0.629982 | 8.1:+10965045 | None:intergenic |
| GCTGGCAACTTCGTCGACGG+CGG | 0.630418 | 8.1:+10965087 | None:intergenic |
| TGCAGAGCTATTACTCCAAG+AGG | 0.630839 | 8.1:-10963380 | MS.gene041376:CDS |
| GAACGACGTGTACACTGCCG+CGG | 0.632469 | 8.1:-10965062 | MS.gene041376:CDS |
| TACCTCGCATAGCAGCCCAA+TGG | 0.633795 | 8.1:+10961043 | None:intergenic |
| AGAATCAGGTAACTCTGAGG+AGG | 0.634143 | 8.1:-10956866 | MS.gene041376:CDS |
| ACTTCAATTACCAATGCAAG+GGG | 0.636012 | 8.1:+10963014 | None:intergenic |
| CATTGTAGTCATAGTAACCA+AGG | 0.642884 | 8.1:-10956670 | MS.gene041376:CDS |
| GGGTTTCTGAATCTACCACC+TGG | 0.646328 | 8.1:+10956964 | None:intergenic |
| TTATTTCTCGATGCACATCG+GGG | 0.647180 | 8.1:-10961223 | MS.gene041376:CDS |
| TTTGCCAGCCTGTACTAACA+CGG | 0.650416 | 8.1:+10961005 | None:intergenic |
| TCTCATTCTGATCATGCCAA+CGG | 0.655122 | 8.1:-10956787 | MS.gene041376:CDS |
| GGAATGATGCACATGGCAAG+GGG | 0.657355 | 8.1:-10956838 | MS.gene041376:CDS |
| GAATCAGGTAACTCTGAGGA+GGG | 0.665015 | 8.1:-10956865 | MS.gene041376:CDS |
| AATCCACATTAATAACAACA+TGG | 0.665996 | 8.1:+10956752 | None:intergenic |
| AGAAATGACAGCATTCCTGT+GGG | 0.666053 | 8.1:-10956640 | MS.gene041376:CDS |
| CTGATACCCATTCATGTCAG+CGG | 0.667732 | 8.1:+10963342 | None:intergenic |
| CGAGATCGAGGTCGTCGAGG+AGG | 0.670692 | 8.1:-10965158 | MS.gene041376:CDS |
| TTCCGAGATCGAGGTCGTCG+AGG | 0.672079 | 8.1:-10965161 | MS.gene041376:CDS |
| AGCAGCCCAATGGAGAGGAG+TGG | 0.672394 | 8.1:+10961053 | None:intergenic |
| TGTACCGTGTTAGTACAGGC+TGG | 0.673682 | 8.1:-10961009 | MS.gene041376:CDS |
| AGCTACCTCAGAGGTCCAGG+TGG | 0.674547 | 8.1:-10956979 | MS.gene041376:CDS |
| ATTTAGATATCTTTCCAAGG+CGG | 0.677729 | 8.1:+10959739 | None:intergenic |
| TATTTCTCGATGCACATCGG+GGG | 0.678336 | 8.1:-10961222 | MS.gene041376:CDS |
| ATGAAATGGCAAATGCATTG+CGG | 0.679208 | 8.1:-10957005 | MS.gene041376:CDS |
| GCAGAGCTATTACTCCAAGA+GGG | 0.681123 | 8.1:-10963379 | MS.gene041376:CDS |
| ATCTACCACCTGGACCTCTG+AGG | 0.691439 | 8.1:+10956974 | None:intergenic |
| CCAATGCAATTGGATACCCA+AGG | 0.692775 | 8.1:+10958167 | None:intergenic |
| GGGTCTAGGAATGATGCACA+TGG | 0.698036 | 8.1:-10956845 | MS.gene041376:CDS |
| TTGCGGTATAGCTACCTCAG+AGG | 0.721082 | 8.1:-10956988 | MS.gene041376:CDS |
| GCGGAAGATGATGATGATGG+GGG | 0.722715 | 8.1:+10965109 | None:intergenic |
| TGAAGAATCAGGTAACTCTG+AGG | 0.725936 | 8.1:-10956869 | MS.gene041376:CDS |
| GGTATTGTTTGGCAGCATGG+TGG | 0.728517 | 8.1:-10963475 | MS.gene041376:intron |
| GACATGTCATTATCACGACG+AGG | 0.733985 | 8.1:+10956576 | None:intergenic |
| TGTACTGAGCAGCAGCAGTG+CGG | 0.750685 | 8.1:+10964930 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 10956588 | 10965188 | 10956588 | ID=MS.gene041376 |
| chr8.1 | mRNA | 10956588 | 10965188 | 10956588 | ID=MS.gene041376.t1;Parent=MS.gene041376 |
| chr8.1 | exon | 10964922 | 10965188 | 10964922 | ID=MS.gene041376.t1.exon1;Parent=MS.gene041376.t1 |
| chr8.1 | CDS | 10964922 | 10965188 | 10964922 | ID=cds.MS.gene041376.t1;Parent=MS.gene041376.t1 |
| chr8.1 | exon | 10963342 | 10963482 | 10963342 | ID=MS.gene041376.t1.exon2;Parent=MS.gene041376.t1 |
| chr8.1 | CDS | 10963342 | 10963482 | 10963342 | ID=cds.MS.gene041376.t1;Parent=MS.gene041376.t1 |
| chr8.1 | exon | 10963025 | 10963137 | 10963025 | ID=MS.gene041376.t1.exon3;Parent=MS.gene041376.t1 |
| chr8.1 | CDS | 10963025 | 10963137 | 10963025 | ID=cds.MS.gene041376.t1;Parent=MS.gene041376.t1 |
| chr8.1 | exon | 10961206 | 10961285 | 10961206 | ID=MS.gene041376.t1.exon4;Parent=MS.gene041376.t1 |
| chr8.1 | CDS | 10961206 | 10961285 | 10961206 | ID=cds.MS.gene041376.t1;Parent=MS.gene041376.t1 |
| chr8.1 | exon | 10960915 | 10961078 | 10960915 | ID=MS.gene041376.t1.exon5;Parent=MS.gene041376.t1 |
| chr8.1 | CDS | 10960915 | 10961078 | 10960915 | ID=cds.MS.gene041376.t1;Parent=MS.gene041376.t1 |
| chr8.1 | exon | 10959667 | 10959805 | 10959667 | ID=MS.gene041376.t1.exon6;Parent=MS.gene041376.t1 |
| chr8.1 | CDS | 10959667 | 10959805 | 10959667 | ID=cds.MS.gene041376.t1;Parent=MS.gene041376.t1 |
| chr8.1 | exon | 10959437 | 10959536 | 10959437 | ID=MS.gene041376.t1.exon7;Parent=MS.gene041376.t1 |
| chr8.1 | CDS | 10959437 | 10959536 | 10959437 | ID=cds.MS.gene041376.t1;Parent=MS.gene041376.t1 |
| chr8.1 | exon | 10958658 | 10958715 | 10958658 | ID=MS.gene041376.t1.exon8;Parent=MS.gene041376.t1 |
| chr8.1 | CDS | 10958658 | 10958715 | 10958658 | ID=cds.MS.gene041376.t1;Parent=MS.gene041376.t1 |
| chr8.1 | exon | 10958401 | 10958478 | 10958401 | ID=MS.gene041376.t1.exon9;Parent=MS.gene041376.t1 |
| chr8.1 | CDS | 10958401 | 10958478 | 10958401 | ID=cds.MS.gene041376.t1;Parent=MS.gene041376.t1 |
| chr8.1 | exon | 10958163 | 10958261 | 10958163 | ID=MS.gene041376.t1.exon10;Parent=MS.gene041376.t1 |
| chr8.1 | CDS | 10958163 | 10958261 | 10958163 | ID=cds.MS.gene041376.t1;Parent=MS.gene041376.t1 |
| chr8.1 | exon | 10957379 | 10957457 | 10957379 | ID=MS.gene041376.t1.exon11;Parent=MS.gene041376.t1 |
| chr8.1 | CDS | 10957379 | 10957457 | 10957379 | ID=cds.MS.gene041376.t1;Parent=MS.gene041376.t1 |
| chr8.1 | exon | 10957146 | 10957294 | 10957146 | ID=MS.gene041376.t1.exon12;Parent=MS.gene041376.t1 |
| chr8.1 | CDS | 10957146 | 10957294 | 10957146 | ID=cds.MS.gene041376.t1;Parent=MS.gene041376.t1 |
| chr8.1 | exon | 10956588 | 10957049 | 10956588 | ID=MS.gene041376.t1.exon13;Parent=MS.gene041376.t1 |
| chr8.1 | CDS | 10956588 | 10957049 | 10956588 | ID=cds.MS.gene041376.t1;Parent=MS.gene041376.t1 |
| Gene Sequence |
| Protein sequence |
