AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene027672

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027672.t1 MTR_5g033700 98.780 246 3 0 1 246 17 262 0.0 503
MS.gene027672.t1 MTR_4g009880 28.632 234 148 4 25 239 49 282 3.90e-23 95.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene027672.t1 AT1G23360 69.198 237 71 1 10 244 23 259 6.65e-120 342
MS.gene027672.t1 AT1G23360 64.557 158 54 1 89 244 1 158 2.47e-70 213
MS.gene027672.t1 AT1G23360 64.557 158 54 1 89 244 1 158 2.47e-70 213
MS.gene027672.t1 AT5G57300 28.151 238 147 4 25 241 48 282 2.70e-22 93.2
MS.gene027672.t1 AT5G57300 28.151 238 147 4 25 241 48 282 2.70e-22 93.2
MS.gene027672.t1 AT5G57300 28.151 238 147 4 25 241 48 282 2.70e-22 93.2
MS.gene027672.t1 AT5G57300 28.151 238 147 4 25 241 48 282 2.70e-22 93.2
MS.gene027672.t1 AT2G41040 26.906 223 126 7 26 215 133 351 1.55e-11 63.5

Find 56 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCCCTGTAGCCTCTGTTTA+TGG 0.157587 5.2:-23727828 MS.gene027672:CDS
GCTACTAACTTCTTCATTTA+CGG 0.259722 5.2:-23728041 MS.gene027672:intron
GCTTGGGTCAGCACCGGATT+TGG 0.335534 5.2:-23729812 MS.gene027672:CDS
GGAAGCGAATGGCTGTTTCT+TGG 0.344170 5.2:-23729791 MS.gene027672:CDS
GCTGAATGATTTGCTGAGCT+TGG 0.375216 5.2:-23729829 MS.gene027672:CDS
GAGAAACTTGCTTTAGAAGT+AGG 0.398667 5.2:-23727563 MS.gene027672:CDS
CAGCCATTCGCTTCCAAATC+CGG 0.402703 5.2:+23729799 None:intergenic
TGGAGGCCTCATGGGGTGCT+TGG 0.422234 5.2:-23727510 MS.gene027672:CDS
CTGTGATGCAGGGAAGGAAT+TGG 0.426333 5.2:-23727585 MS.gene027672:intron
TTGGTTTGATGCAATCACTA+TGG 0.429752 5.2:-23728253 MS.gene027672:CDS
TTTCTCAAAGGAGCAACTAA+TGG 0.435316 5.2:-23728618 MS.gene027672:CDS
GAGATCGGTGGAGGCCTCAT+GGG 0.444479 5.2:-23727518 MS.gene027672:CDS
CTGAATGATTTGCTGAGCTT+GGG 0.456434 5.2:-23729828 MS.gene027672:CDS
ATGGAAGTCGGTCGGAATGA+AGG 0.467617 5.2:+23729987 None:intergenic
AGAAGAGCTTGCCTTGCCAT+TGG 0.469398 5.2:+23729596 None:intergenic
TGCTGAGCTTGGGTCAGCAC+CGG 0.478547 5.2:-23729818 MS.gene027672:CDS
AGAGCTTGCCTTGCCATTGG+AGG 0.480628 5.2:+23729599 None:intergenic
GTACATCCTAGGTGGGTTGA+AGG 0.482364 5.2:-23728306 MS.gene027672:intron
TTGTGTTGCAGAGATGTTCC+CGG 0.492822 5.2:-23730024 None:intergenic
TGAGATCGGTGGAGGCCTCA+TGG 0.504020 5.2:-23727519 MS.gene027672:CDS
GAAAAGCGCCTGTCGGTCGT+TGG 0.508235 5.2:+23729956 None:intergenic
AGGCCATAAACAGAGGCTAC+AGG 0.514026 5.2:+23727825 None:intergenic
TTGGATGTCTGCTGTGGAAG+TGG 0.515717 5.2:-23729654 MS.gene027672:CDS
AAGGATAAGGAGCAACCGCC+GGG 0.515863 5.2:+23730006 None:intergenic
TGGTTTGATGCAATCACTAT+GGG 0.517163 5.2:-23728252 MS.gene027672:CDS
TCAAACCAAGCATCAGAAAA+CGG 0.523537 5.2:+23728267 None:intergenic
TCGCTGCTCCAACGACCGAC+AGG 0.524223 5.2:-23729964 MS.gene027672:CDS
CGACATTATGAGATCGGTGG+AGG 0.533048 5.2:-23727527 MS.gene027672:CDS
GTCGGTCGGAATGAAGGATA+AGG 0.536675 5.2:+23729993 None:intergenic
GATGCAATCACTATGGGCTA+TGG 0.547081 5.2:-23728246 MS.gene027672:CDS
GGGCGCACGTTATCATAGAC+AGG 0.548668 5.2:+23729921 None:intergenic
AACTTTGTTAGACAAGAGAA+AGG 0.551476 5.2:+23729623 None:intergenic
CTTTGAAAGCGACCGTGATG+AGG 0.553016 5.2:+23728592 None:intergenic
TCTAACAAAGTTGCCTCCAA+TGG 0.553337 5.2:-23729612 MS.gene027672:CDS
GAAGGATAAGGAGCAACCGC+CGG 0.567694 5.2:+23730005 None:intergenic
TGATAAGCGTAAAGCCATGC+AGG 0.572450 5.2:-23728208 MS.gene027672:CDS
GATATTTGTAGTCATCTGAA+AGG 0.577823 5.2:+23727805 None:intergenic
TCGGTCGTTGGAGCAGCGAA+TGG 0.583040 5.2:+23729968 None:intergenic
GGCCATAAACAGAGGCTACA+GGG 0.586319 5.2:+23727826 None:intergenic
CACACGTAAAATTTCCTGCA+TGG 0.586402 5.2:+23728194 None:intergenic
ACTGCTCGACATTATGAGAT+CGG 0.594791 5.2:-23727533 MS.gene027672:CDS
GATTGGTCTTGATTTCTCAA+AGG 0.599953 5.2:-23728630 MS.gene027672:CDS
CAAAGTTGCCTCCAATGGCA+AGG 0.615328 5.2:-23729607 MS.gene027672:intron
GCACCGGATTTGGAAGCGAA+TGG 0.615773 5.2:-23729802 MS.gene027672:CDS
TGCGGTTGAAAAGCGCCTGT+CGG 0.616608 5.2:+23729949 None:intergenic
AGATCGGTGGAGGCCTCATG+GGG 0.618606 5.2:-23727517 MS.gene027672:CDS
AACTAATGGTCGCCTCATCA+CGG 0.620553 5.2:-23728604 MS.gene027672:CDS
TTGGAGCAGCGAATGGAAGT+CGG 0.628117 5.2:+23729975 None:intergenic
CGTGTATTGGATGTCTGCTG+TGG 0.636133 5.2:-23729660 MS.gene027672:CDS
CACAGCAGACATCCAATACA+CGG 0.640805 5.2:+23729661 None:intergenic
AGCAGCGAATGGAAGTCGGT+CGG 0.656578 5.2:+23729979 None:intergenic
ATCTGAAAGGCCATAAACAG+AGG 0.659783 5.2:+23727818 None:intergenic
GCTCGACATTATGAGATCGG+TGG 0.666601 5.2:-23727530 MS.gene027672:CDS
TAGCTACCAAGCACCCCATG+AGG 0.708176 5.2:+23727504 None:intergenic
TATCATAGACAGGAGCAATG+CGG 0.761354 5.2:+23729931 None:intergenic
TGTTGCAGAGATGTTCCCGG+CGG 0.792432 5.2:-23730021 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATATTTTTCAAAAAAAAAA+AGG + chr5.2:23728099-23728118 None:intergenic 10.0%
!! ATACTAATGTATAGTATAAT+AGG - chr5.2:23729162-23729181 MS.gene027672:intron 15.0%
!! TACTAATGTATAGTATAATA+GGG - chr5.2:23729163-23729182 MS.gene027672:intron 15.0%
!!! ATTTTTTTTATAGTAGTAAG+AGG - chr5.2:23729829-23729848 MS.gene027672:CDS 15.0%
!! ATAATATTGCAAAAATGAGT+TGG + chr5.2:23728125-23728144 None:intergenic 20.0%
!! ATACTATACATTAGTATCAT+TGG + chr5.2:23729159-23729178 None:intergenic 20.0%
!! ATATAATTAATGTGTTGTGA+AGG - chr5.2:23728192-23728211 MS.gene027672:CDS 20.0%
!! ATCATTTCATTCTATTCTAA+TGG - chr5.2:23727768-23727787 MS.gene027672:intron 20.0%
!! GTTAATGTATCTTGAATAAT+CGG + chr5.2:23728749-23728768 None:intergenic 20.0%
!! TAAAATAGTTAAATGAGACA+TGG - chr5.2:23728159-23728178 MS.gene027672:intron 20.0%
!! TATAATTAATGTGTTGTGAA+GGG - chr5.2:23728193-23728212 MS.gene027672:CDS 20.0%
!! TATTATAGACCAAATACAAT+AGG - chr5.2:23728377-23728396 MS.gene027672:intron 20.0%
!! TTATTCAAGATACATTAACA+TGG - chr5.2:23728750-23728769 MS.gene027672:intron 20.0%
!!! TAAGCAAAAAAACACTTTTT+AGG + chr5.2:23728414-23728433 None:intergenic 20.0%
!!! TAAGCAAAAAATCACTTTTT+AGG - chr5.2:23728325-23728344 MS.gene027672:intron 20.0%
!!! TCAATAAGAGAATTTTTAAC+AGG - chr5.2:23729733-23729752 MS.gene027672:intron 20.0%
!!! TCATTTTTGCAATATTATGT+TGG - chr5.2:23728127-23728146 MS.gene027672:intron 20.0%
!!! TTAGTGTTTTAGCTTTATAA+CGG + chr5.2:23729074-23729093 None:intergenic 20.0%
! AAGTTGTACATTAGTATCAT+TGG - chr5.2:23729120-23729139 MS.gene027672:intron 25.0%
! ATCGGTTAATTGTTTAAATG+CGG + chr5.2:23728731-23728750 None:intergenic 25.0%
! CAAGTCAATAACATATTGAT+TGG - chr5.2:23729361-23729380 MS.gene027672:intron 25.0%
! CATTCAACACTTAATGTATT+TGG - chr5.2:23728350-23728369 MS.gene027672:intron 25.0%
! TTGATTGTTGTGTTTGAATT+TGG - chr5.2:23727802-23727821 MS.gene027672:CDS 25.0%
! TTTAATCATTTGTACATCCT+AGG - chr5.2:23729190-23729209 MS.gene027672:intron 25.0%
!! AAGCTTTATAAACACTACAT+AGG + chr5.2:23728526-23728545 None:intergenic 25.0%
!! CAGCTTTATAAACACTAAAT+AGG + chr5.2:23728674-23728693 None:intergenic 25.0%
!! TTTTTGCTTATAAATGTGAC+CGG - chr5.2:23728424-23728443 MS.gene027672:intron 25.0%
!! TTTTTGCTTATAAATGTGAC+CGG + chr5.2:23728443-23728424 None:intergenic 25.0%
!!! CATTGCTTTTATTAAAGTCA+AGG + chr5.2:23729445-23729464 None:intergenic 25.0%
!!! TTTGTTTTCAATTGAACTGA+AGG - chr5.2:23729756-23729775 MS.gene027672:intron 25.0%
AACCAACCCAAATTCTTTAT+CGG - chr5.2:23728587-23728606 MS.gene027672:CDS 30.0%
AACTTTGTTAGACAAGAGAA+AGG + chr5.2:23727887-23727906 None:intergenic 30.0%
ACAAGACAACAAATCCTTTA+TGG - chr5.2:23728779-23728798 MS.gene027672:intron 30.0%
AGAAAGAGAAAGAAAGAAGA+AGG + chr5.2:23727610-23727629 None:intergenic 30.0%
ATCCCAAAAGATTCACAAAA+CGG + chr5.2:23728851-23728870 None:intergenic 30.0%
CATTCAACACCTATTGTATT+TGG + chr5.2:23728389-23728408 None:intergenic 30.0%
GAAAGAGAAAGAAAGAAGAA+GGG + chr5.2:23727609-23727628 None:intergenic 30.0%
GATATTTGTAGTCATCTGAA+AGG + chr5.2:23729705-23729724 None:intergenic 30.0%
GCAAAACATAAATGTTGCAA+GGG + chr5.2:23729496-23729515 None:intergenic 30.0%
GCTACTAACTTCTTCATTTA+CGG - chr5.2:23729466-23729485 MS.gene027672:intron 30.0%
TCAATAATATTGAGTGAGCA+GGG - chr5.2:23728945-23728964 MS.gene027672:intron 30.0%
TCTCTCGTAAAAATACTTTC+AGG + chr5.2:23729005-23729024 None:intergenic 30.0%
TGCAAACAAAATGTCCATAA+AGG + chr5.2:23728796-23728815 None:intergenic 30.0%
TGGAATAAAATCGGGTTTAT+TGG - chr5.2:23728216-23728235 MS.gene027672:CDS 30.0%
TGTTGAAAAATATGTTGCCA+CGG - chr5.2:23727954-23727973 MS.gene027672:intron 30.0%
TTCAATAATATTGAGTGAGC+AGG - chr5.2:23728944-23728963 MS.gene027672:intron 30.0%
! ATTTGGTAACAGAGCAAAAA+AGG - chr5.2:23727819-23727838 MS.gene027672:CDS 30.0%
! TTTGGTAACAGAGCAAAAAA+GGG - chr5.2:23727820-23727839 MS.gene027672:CDS 30.0%
!! CACAGGAGTATTATTAAGTT+AGG + chr5.2:23729907-23729926 None:intergenic 30.0%
AATAATACTCCTGTGATGCA+GGG - chr5.2:23729912-23729931 MS.gene027672:intron 35.0%
AATCATTTGTACATCCTAGG+TGG - chr5.2:23729193-23729212 MS.gene027672:intron 35.0%
AATTAATGTGTTGTGAAGGG+TGG - chr5.2:23728196-23728215 MS.gene027672:CDS 35.0%
ATCATTTGTACATCCTAGGT+GGG - chr5.2:23729194-23729213 MS.gene027672:intron 35.0%
ATCCGATAAAGAATTTGGGT+TGG + chr5.2:23728592-23728611 None:intergenic 35.0%
ATTCAGCTGCAACAATGAAA+AGG + chr5.2:23727665-23727684 None:intergenic 35.0%
CAAAAAAAAAAAGGGCAACC+CGG + chr5.2:23728090-23728109 None:intergenic 35.0%
CAATAATATTGAGTGAGCAG+GGG - chr5.2:23728946-23728965 MS.gene027672:intron 35.0%
CCAAAGAATCAAAAAGACGA+AGG + chr5.2:23729599-23729618 None:intergenic 35.0%
CGCAAAACATAAATGTTGCA+AGG + chr5.2:23729497-23729516 None:intergenic 35.0%
GTTGAAAAATATGTTGCCAC+GGG - chr5.2:23727955-23727974 MS.gene027672:intron 35.0%
TAATAATACTCCTGTGATGC+AGG - chr5.2:23729911-23729930 MS.gene027672:intron 35.0%
TAGTTAAATGAGACATGGTG+AGG - chr5.2:23728164-23728183 MS.gene027672:intron 35.0%
TCAAACCAAGCATCAGAAAA+CGG + chr5.2:23729243-23729262 None:intergenic 35.0%
TCAGGCTGTTATCTAATTTC+AGG + chr5.2:23728987-23729006 None:intergenic 35.0%
TCTTGTGTAAAAACAGGGTA+AGG - chr5.2:23727998-23728017 MS.gene027672:intron 35.0%
TGGGATCCGATAAAGAATTT+GGG + chr5.2:23728596-23728615 None:intergenic 35.0%
TGGTTTGATGCAATCACTAT+GGG - chr5.2:23729255-23729274 MS.gene027672:intron 35.0%
TGTGAAGGGTGGAATAAAAT+CGG - chr5.2:23728207-23728226 MS.gene027672:CDS 35.0%
TGTTCCAATTTACGCTTGAT+CGG + chr5.2:23729789-23729808 None:intergenic 35.0%
TTATCCGATCAAGCGTAAAT+TGG - chr5.2:23729782-23729801 MS.gene027672:intron 35.0%
TTGGGATCCGATAAAGAATT+TGG + chr5.2:23728597-23728616 None:intergenic 35.0%
TTTCTCAAAGGAGCAACTAA+TGG - chr5.2:23728889-23728908 MS.gene027672:intron 35.0%
! CACCGTTTTGTGAATCTTTT+GGG - chr5.2:23728846-23728865 MS.gene027672:intron 35.0%
! TCACCGTTTTGTGAATCTTT+TGG - chr5.2:23728845-23728864 MS.gene027672:intron 35.0%
! TTGGTTTGATGCAATCACTA+TGG - chr5.2:23729254-23729273 MS.gene027672:intron 35.0%
!! CCTTCGTCTTTTTGATTCTT+TGG - chr5.2:23729596-23729615 MS.gene027672:intron 35.0%
!! GAGAAACTTGCTTTAGAAGT+AGG - chr5.2:23729944-23729963 MS.gene027672:CDS 35.0%
!! GATTGGTCTTGATTTCTCAA+AGG - chr5.2:23728877-23728896 MS.gene027672:intron 35.0%
!! TAGCTTTATAACGGAGAACA+TGG + chr5.2:23729065-23729084 None:intergenic 35.0%
!! TTTTGATTCTTTGGTGTGAG+CGG - chr5.2:23729605-23729624 MS.gene027672:intron 35.0%
!!! TTACCCTGTTTTTACACAAG+AGG + chr5.2:23727999-23728018 None:intergenic 35.0%
!!! TTGTGAATCTTTTGGGATGT+AGG - chr5.2:23728853-23728872 MS.gene027672:intron 35.0%
AAAAAAGGGTGACCGTGTAT+TGG - chr5.2:23727834-23727853 MS.gene027672:CDS 40.0%
AAATAGGCCCATATCTCTGT+GGG + chr5.2:23728658-23728677 None:intergenic 40.0%
ACAGCCTCTTGTGTAAAAAC+AGG - chr5.2:23727992-23728011 MS.gene027672:intron 40.0%
ACTACATAGGTCGTATCTCT+AGG + chr5.2:23728513-23728532 None:intergenic 40.0%
ACTGCTCGACATTATGAGAT+CGG - chr5.2:23729974-23729993 MS.gene027672:CDS 40.0%
ACTTGAGATACAACAGCTAC+CGG + chr5.2:23729346-23729365 None:intergenic 40.0%
ATCTGAAAGGCCATAAACAG+AGG + chr5.2:23729692-23729711 None:intergenic 40.0%
CACACGTAAAATTTCCTGCA+TGG + chr5.2:23729316-23729335 None:intergenic 40.0%
CAGCCTCTTGTGTAAAAACA+GGG - chr5.2:23727993-23728012 MS.gene027672:intron 40.0%
CCTTTCATACCCAAAACGTT+GGG + chr5.2:23728615-23728634 None:intergenic 40.0%
CGTACAATACACCAAATGGT+GGG - chr5.2:23728024-23728043 MS.gene027672:intron 40.0%
CTACATAGGTCGTATCTCTA+GGG + chr5.2:23728512-23728531 None:intergenic 40.0%
GTGAAGGGTGGAATAAAATC+GGG - chr5.2:23728208-23728227 MS.gene027672:CDS 40.0%
TAAATAGGCCCATATCTCTG+TGG + chr5.2:23728659-23728678 None:intergenic 40.0%
TATCATAGACAGGAGCAATG+CGG + chr5.2:23727579-23727598 None:intergenic 40.0%
TCTAACAAAGTTGCCTCCAA+TGG - chr5.2:23727895-23727914 MS.gene027672:intron 40.0%
TGCTTATAAATGTGACCGGA+GGG - chr5.2:23728428-23728447 MS.gene027672:intron 40.0%
TGCTTATAAATGTGACCGGA+GGG + chr5.2:23728447-23728428 None:intergenic 40.0%
TGGAAATTCCACCTTCATTG+CGG - chr5.2:23728236-23728255 MS.gene027672:CDS 40.0%
TGGGTGTGAATGAGTGAATT+TGG - chr5.2:23728477-23728496 MS.gene027672:intron 40.0%
TTGCTTATAAATGTGACCGG+AGG - chr5.2:23728427-23728446 MS.gene027672:intron 40.0%
TTGCTTATAAATGTGACCGG+AGG + chr5.2:23728446-23728427 None:intergenic 40.0%
TTTGCGCTGCTTAAGTTTAG+AGG - chr5.2:23729511-23729530 MS.gene027672:intron 40.0%
! GCAACAATGAAAAGGTGTGA+AGG + chr5.2:23727657-23727676 None:intergenic 40.0%
! TTATCGGATCCCAACGTTTT+GGG - chr5.2:23728603-23728622 MS.gene027672:CDS 40.0%
! TTTATCGGATCCCAACGTTT+TGG - chr5.2:23728602-23728621 MS.gene027672:CDS 40.0%
!! CCAACGTTTTGGGTATGAAA+GGG - chr5.2:23728613-23728632 MS.gene027672:CDS 40.0%
!! CTGAATGATTTGCTGAGCTT+GGG - chr5.2:23727679-23727698 MS.gene027672:intron 40.0%
!!! CGTGCTACTTTTTACTTTCC+AGG - chr5.2:23729404-23729423 MS.gene027672:intron 40.0%
!!! TCTTTTGGGATGTAGGTGAT+TGG - chr5.2:23728860-23728879 MS.gene027672:intron 40.0%
AACTAATGGTCGCCTCATCA+CGG - chr5.2:23728903-23728922 MS.gene027672:intron 45.0%
AGGATAGATGCTGTAGAACC+TGG + chr5.2:23729425-23729444 None:intergenic 45.0%
CACAGCAGACATCCAATACA+CGG + chr5.2:23727849-23727868 None:intergenic 45.0%
CACATTGCCCACAGAGATAT+GGG - chr5.2:23728648-23728667 MS.gene027672:intron 45.0%
CACTCCTAGAACGAGAAATG+GGG + chr5.2:23729640-23729659 None:intergenic 45.0%
CCACTCCTAGAACGAGAAAT+GGG + chr5.2:23729641-23729660 None:intergenic 45.0%
CCCTTTCATACCCAAAACGT+TGG + chr5.2:23728616-23728635 None:intergenic 45.0%
CTAACCCCATTTCTCGTTCT+AGG - chr5.2:23729633-23729652 MS.gene027672:CDS 45.0%
CTTGAGATACAACAGCTACC+GGG + chr5.2:23729345-23729364 None:intergenic 45.0%
GAAACCACCTTGTAAGGTGA+TGG + chr5.2:23728555-23728574 None:intergenic 45.0%
GCGTACAATACACCAAATGG+TGG - chr5.2:23728023-23728042 MS.gene027672:intron 45.0%
GCTGCGTACAATACACCAAA+TGG - chr5.2:23728020-23728039 MS.gene027672:intron 45.0%
GGAGTAGCTTCCAACACATT+CGG - chr5.2:23728449-23728468 MS.gene027672:intron 45.0%
TACCCAACCATCACCTTACA+AGG - chr5.2:23728545-23728564 MS.gene027672:intron 45.0%
TCACCTTACAAGGTGGTTTC+CGG - chr5.2:23728555-23728574 MS.gene027672:intron 45.0%
TCCACTCCTAGAACGAGAAA+TGG + chr5.2:23729642-23729661 None:intergenic 45.0%
TGATAAGCGTAAAGCCATGC+AGG - chr5.2:23729299-23729318 MS.gene027672:intron 45.0%
! ATTTTACGTGTGCTGAAGCC+CGG - chr5.2:23729324-23729343 MS.gene027672:intron 45.0%
! CACCTTGTAAGGTGATGGTT+GGG + chr5.2:23728550-23728569 None:intergenic 45.0%
! GATGCAATCACTATGGGCTA+TGG - chr5.2:23729261-23729280 MS.gene027672:intron 45.0%
! GTCGTATCTCTAGGGAATGT+GGG + chr5.2:23728504-23728523 None:intergenic 45.0%
! TTACACAAGAGGCTGTTTCC+AGG + chr5.2:23727988-23728007 None:intergenic 45.0%
!! CCCAACGTTTTGGGTATGAA+AGG - chr5.2:23728612-23728631 MS.gene027672:CDS 45.0%
!! GCTGAATGATTTGCTGAGCT+TGG - chr5.2:23727678-23727697 MS.gene027672:intron 45.0%
!! TCCAACACATTCGGTGTGTT+GGG - chr5.2:23728458-23728477 MS.gene027672:intron 45.0%
!! TGGGTTGGTTCTAACTCAAC+CGG + chr5.2:23728577-23728596 None:intergenic 45.0%
!! TTCCAACACATTCGGTGTGT+TGG - chr5.2:23728457-23728476 MS.gene027672:intron 45.0%
!!! ACTTGCCGTTTTCTGATGCT+TGG - chr5.2:23729235-23729254 MS.gene027672:intron 45.0%
!!! ATATTTTTCAAAAAAAAAAA+GGG + chr5.2:23728098-23728117 None:intergenic 5.0%
ACCCAACACACCGAATGTGT+TGG + chr5.2:23728462-23728481 None:intergenic 50.0%
AGAAGAGCTTGCCTTGCCAT+TGG + chr5.2:23727914-23727933 None:intergenic 50.0%
AGGCCATAAACAGAGGCTAC+AGG + chr5.2:23729685-23729704 None:intergenic 50.0%
ATACTCCTGTGATGCAGGGA+AGG - chr5.2:23729916-23729935 MS.gene027672:intron 50.0%
ATGGAAGTCGGTCGGAATGA+AGG + chr5.2:23727523-23727542 None:intergenic 50.0%
CAAAGTTGCCTCCAATGGCA+AGG - chr5.2:23727900-23727919 MS.gene027672:intron 50.0%
CAACCGGAAACCACCTTGTA+AGG + chr5.2:23728561-23728580 None:intergenic 50.0%
CAATTCCTTCCCTGCATCAC+AGG + chr5.2:23729924-23729943 None:intergenic 50.0%
CAGCCATTCGCTTCCAAATC+CGG + chr5.2:23727711-23727730 None:intergenic 50.0%
CCAACCATCACCTTACAAGG+TGG - chr5.2:23728548-23728567 MS.gene027672:intron 50.0%
CCACATTGCCCACAGAGATA+TGG - chr5.2:23728647-23728666 MS.gene027672:intron 50.0%
CCATATCTCTGTGGGCAATG+TGG + chr5.2:23728650-23728669 None:intergenic 50.0%
CGACATTATGAGATCGGTGG+AGG - chr5.2:23729980-23729999 MS.gene027672:CDS 50.0%
CGTGTATTGGATGTCTGCTG+TGG - chr5.2:23727847-23727866 MS.gene027672:CDS 50.0%
CTGTGATGCAGGGAAGGAAT+TGG - chr5.2:23729922-23729941 MS.gene027672:intron 50.0%
GCTCGACATTATGAGATCGG+TGG - chr5.2:23729977-23729996 MS.gene027672:CDS 50.0%
GGCCATAAACAGAGGCTACA+GGG + chr5.2:23729684-23729703 None:intergenic 50.0%
GTCCCTGTAGCCTCTGTTTA+TGG - chr5.2:23729679-23729698 MS.gene027672:CDS 50.0%
GTCGGTCGGAATGAAGGATA+AGG + chr5.2:23727517-23727536 None:intergenic 50.0%
TGCCTTCATTGTACTCCCTC+CGG - chr5.2:23728293-23728312 MS.gene027672:CDS 50.0%
TTGGAGCAGCGAATGGAAGT+CGG + chr5.2:23727535-23727554 None:intergenic 50.0%
TTGGATGTCTGCTGTGGAAG+TGG - chr5.2:23727853-23727872 MS.gene027672:CDS 50.0%
! CCACCTTGTAAGGTGATGGT+TGG + chr5.2:23728551-23728570 None:intergenic 50.0%
! CTTTGAAAGCGACCGTGATG+AGG + chr5.2:23728918-23728937 None:intergenic 50.0%
! GATTCTTTGGTGTGAGCGGA+AGG - chr5.2:23729609-23729628 MS.gene027672:CDS 50.0%
! GGTCGTATCTCTAGGGAATG+TGG + chr5.2:23728505-23728524 None:intergenic 50.0%
!! CCCATTTCTCGTTCTAGGAG+TGG - chr5.2:23729638-23729657 MS.gene027672:CDS 50.0%
!! GGAAGCGAATGGCTGTTTCT+TGG - chr5.2:23727716-23727735 MS.gene027672:intron 50.0%
!! GTACATCCTAGGTGGGTTGA+AGG - chr5.2:23729201-23729220 MS.gene027672:intron 50.0%
AACGCCGTAATGAGGCAGCT+AGG + chr5.2:23728268-23728287 None:intergenic 55.0%
AAGGATAAGGAGCAACCGCC+GGG + chr5.2:23727504-23727523 None:intergenic 55.0%
ACGCCGTAATGAGGCAGCTA+GGG + chr5.2:23728267-23728286 None:intergenic 55.0%
ACTGAAGCTCCCGCATATGC+AGG + chr5.2:23728064-23728083 None:intergenic 55.0%
AGAGCTTGCCTTGCCATTGG+AGG + chr5.2:23727911-23727930 None:intergenic 55.0%
AGCAGCGAATGGAAGTCGGT+CGG + chr5.2:23727531-23727550 None:intergenic 55.0%
AGCTGCCTCATTACGGCGTT+TGG - chr5.2:23728268-23728287 MS.gene027672:CDS 55.0%
CATATGCAGGGTACGGGAAG+GGG + chr5.2:23728051-23728070 None:intergenic 55.0%
CTGAAGCTCCCGCATATGCA+GGG + chr5.2:23728063-23728082 None:intergenic 55.0%
CTTCCCGTACCCTGCATATG+CGG - chr5.2:23728051-23728070 MS.gene027672:CDS 55.0%
GAAGGATAAGGAGCAACCGC+CGG + chr5.2:23727505-23727524 None:intergenic 55.0%
GCAACCCAAACGCCGTAATG+AGG + chr5.2:23728276-23728295 None:intergenic 55.0%
GCACCGGATTTGGAAGCGAA+TGG - chr5.2:23727705-23727724 MS.gene027672:intron 55.0%
GCATATGCAGGGTACGGGAA+GGG + chr5.2:23728052-23728071 None:intergenic 55.0%
GCATCACCTTCAACCCACCT+AGG + chr5.2:23729210-23729229 None:intergenic 55.0%
GCTGCCTCATTACGGCGTTT+GGG - chr5.2:23728269-23728288 MS.gene027672:CDS 55.0%
GGGCGCACGTTATCATAGAC+AGG + chr5.2:23727589-23727608 None:intergenic 55.0%
GTGTTGGAAGCTACTCCCTC+CGG + chr5.2:23728446-23728465 None:intergenic 55.0%
GTTTCCAGGACTTGAACCCG+TGG + chr5.2:23727974-23727993 None:intergenic 55.0%
TAGCTACCAAGCACCCCATG+AGG + chr5.2:23730006-23730025 None:intergenic 55.0%
TGCCCCTAGCTGCCTCATTA+CGG - chr5.2:23728261-23728280 MS.gene027672:CDS 55.0%
TTCCCGTACCCTGCATATGC+GGG - chr5.2:23728052-23728071 MS.gene027672:CDS 55.0%
! GACCGGAGGGAGTACAATGA+AGG + chr5.2:23728298-23728317 None:intergenic 55.0%
!! TGCGGTTGAAAAGCGCCTGT+CGG + chr5.2:23727561-23727580 None:intergenic 55.0%
AGATCGGTGGAGGCCTCATG+GGG - chr5.2:23729990-23730009 MS.gene027672:CDS 60.0%
ATGCGGGAGCTTCAGTGCAC+CGG - chr5.2:23728068-23728087 MS.gene027672:CDS 60.0%
CGCATATGCAGGGTACGGGA+AGG + chr5.2:23728053-23728072 None:intergenic 60.0%
CGCCGTAATGAGGCAGCTAG+GGG + chr5.2:23728266-23728285 None:intergenic 60.0%
CTCCCGCATATGCAGGGTAC+GGG + chr5.2:23728057-23728076 None:intergenic 60.0%
GAGATCGGTGGAGGCCTCAT+GGG - chr5.2:23729989-23730008 MS.gene027672:CDS 60.0%
GCTCCCGCATATGCAGGGTA+CGG + chr5.2:23728058-23728077 None:intergenic 60.0%
GTTGCCACGGGTTCAAGTCC+TGG - chr5.2:23727967-23727986 MS.gene027672:intron 60.0%
TCGGTCGTTGGAGCAGCGAA+TGG + chr5.2:23727542-23727561 None:intergenic 60.0%
TGAGATCGGTGGAGGCCTCA+TGG - chr5.2:23729988-23730007 MS.gene027672:CDS 60.0%
TGCTGAGCTTGGGTCAGCAC+CGG - chr5.2:23727689-23727708 MS.gene027672:intron 60.0%
! CTAGGGGCACACCGCAATGA+AGG + chr5.2:23728250-23728269 None:intergenic 60.0%
! GCTTGGGTCAGCACCGGATT+TGG - chr5.2:23727695-23727714 MS.gene027672:intron 60.0%
!! GAAAAGCGCCTGTCGGTCGT+TGG + chr5.2:23727554-23727573 None:intergenic 60.0%
CGGGAAGGGGTCCCACCATT+TGG + chr5.2:23728038-23728057 None:intergenic 65.0%
GGGGCACACCGCAATGAAGG+TGG + chr5.2:23728247-23728266 None:intergenic 65.0%
TCGCTGCTCCAACGACCGAC+AGG - chr5.2:23727543-23727562 MS.gene027672:CDS 65.0%
TGCGGGAGCTTCAGTGCACC+GGG - chr5.2:23728069-23728088 MS.gene027672:CDS 65.0%
! TGGAGGCCTCATGGGGTGCT+TGG - chr5.2:23729997-23730016 MS.gene027672:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 23727496 23730033 23727496 ID=MS.gene027672
chr5.2 mRNA 23727496 23730033 23727496 ID=MS.gene027672.t1;Parent=MS.gene027672
chr5.2 exon 23729929 23730033 23729929 ID=MS.gene027672.t1.exon1;Parent=MS.gene027672.t1
chr5.2 CDS 23729929 23730033 23729929 ID=cds.MS.gene027672.t1;Parent=MS.gene027672.t1
chr5.2 exon 23729786 23729850 23729786 ID=MS.gene027672.t1.exon2;Parent=MS.gene027672.t1
chr5.2 CDS 23729786 23729850 23729786 ID=cds.MS.gene027672.t1;Parent=MS.gene027672.t1
chr5.2 exon 23729608 23729698 23729608 ID=MS.gene027672.t1.exon3;Parent=MS.gene027672.t1
chr5.2 CDS 23729608 23729698 23729608 ID=cds.MS.gene027672.t1;Parent=MS.gene027672.t1
chr5.2 exon 23728572 23728654 23728572 ID=MS.gene027672.t1.exon4;Parent=MS.gene027672.t1
chr5.2 CDS 23728572 23728654 23728572 ID=cds.MS.gene027672.t1;Parent=MS.gene027672.t1
chr5.2 exon 23728184 23728317 23728184 ID=MS.gene027672.t1.exon5;Parent=MS.gene027672.t1
chr5.2 CDS 23728184 23728317 23728184 ID=cds.MS.gene027672.t1;Parent=MS.gene027672.t1
chr5.2 exon 23728042 23728103 23728042 ID=MS.gene027672.t1.exon6;Parent=MS.gene027672.t1
chr5.2 CDS 23728042 23728103 23728042 ID=cds.MS.gene027672.t1;Parent=MS.gene027672.t1
chr5.2 exon 23727775 23727874 23727775 ID=MS.gene027672.t1.exon7;Parent=MS.gene027672.t1
chr5.2 CDS 23727775 23727874 23727775 ID=cds.MS.gene027672.t1;Parent=MS.gene027672.t1
chr5.2 exon 23727496 23727596 23727496 ID=MS.gene027672.t1.exon8;Parent=MS.gene027672.t1
chr5.2 CDS 23727496 23727596 23727496 ID=cds.MS.gene027672.t1;Parent=MS.gene027672.t1
Gene Sequence

>MS.gene027672

ATGTTCCCGGCGGTTGCTCCTTATCCTTCATTCCGACCGACTTCCATTCGCTGCTCCAACGACCGACAGGCGCTTTTCAACCGCATTGCTCCTGTCTATGATAACGTGCGCCCTTCTTCTTTCTTTCTCTTTCTCTCTCTCTCTCTAGTCTTCTTTCTCCTTCACACCTTTTCATTGTTGCAGCTGAATGATTTGCTGAGCTTGGGTCAGCACCGGATTTGGAAGCGAATGGCTGTTTCTTGGACTGAGTACGTATTTTGCTTCTTCACTTAATCATTTCATTCTATTCTAATGGAATTAATTAATTTGATTGTTGTGTTTGAATTTGGTAACAGAGCAAAAAAGGGTGACCGTGTATTGGATGTCTGCTGTGGAAGTGGTGATTTATCCTTTCTCTTGTCTAACAAAGTTGCCTCCAATGGCAAGGCAAGCTCTTCTTCTCTATCTTTTTTGCATTATGTTGAAAAATATGTTGCCACGGGTTCAAGTCCTGGAAACAGCCTCTTGTGTAAAAACAGGGTAAGGCTGCGTACAATACACCAAATGGTGGGACCCCTTCCCGTACCCTGCATATGCGGGAGCTTCAGTGCACCGGGTTGCCCTTTTTTTTTTTGAAAAATATGTTGCCAACTCATTTTTGCAATATTATGTTGGAGTTGAGATTAAAATAGTTAAATGAGACATGGTGAGGTAAAAATATAATTAATGTGTTGTGAAGGGTGGAATAAAATCGGGTTTATTGGAAATTCCACCTTCATTGCGGTGTGCCCCTAGCTGCCTCATTACGGCGTTTGGGTTGCCTTCATTGTACTCCCTCCGGTCACATTTATAAGCAAAAAATCACTTTTTAGGTTCATTCAACACTTAATGTATTTGGTCTATATTATAGACCAAATACAATAGGTGTTGAATGAACCTAAAAAGTGTTTTTTTGCTTATAAATGTGACCGGAGGGAGTAGCTTCCAACACATTCGGTGTGTTGGGTGTGAATGAGTGAATTTGGTCCCACATTCCCTAGAGATACGACCTATGTAGTGTTTATAAAGCTTACCCAACCATCACCTTACAAGGTGGTTTCCGGTTGAGTTAGAACCAACCCAAATTCTTTATCGGATCCCAACGTTTTGGGTATGAAAGGGTGAGTTTAGTTCCACATTGCCCACAGAGATATGGGCCTATTTAGTGTTTATAAAGCTGACAATGCATTTTATTGTTAATTATTTTAATGATTCCGCATTTAAACAATTAACCGATTATTCAAGATACATTAACATGGTATAGAACAAGACAACAAATCCTTTATGGACATTTTGTTTGCATAAGAATTACTCTAATTTTTGCAATGAAATCACCGTTTTGTGAATCTTTTGGGATGTAGGTGATTGGTCTTGATTTCTCAAAGGAGCAACTAATGGTCGCCTCATCACGGTCGCTTTCAAAGAATTGCTTCAATAATATTGAGTGAGCAGGGGTAACTTTTGCAGTATCCTGAAATTAGATAACAGCCTGAAAGTATTTTTACGAGAGAAAGTTGCTCTGTACACATTTAATTATTCATTTCTTTTCCATGTTCTCCGTTATAAAGCTAAAACACTAATTATAGTGAAGAGTACTCTTGTGATCAAGTTGTACATTAGTATCATTGGTGATAGTCTATCACCAATGATACTAATGTATAGTATAATAGGGAGATTTTAATCATTTGTACATCCTAGGTGGGTTGAAGGTGATGCTCTTGACTTGCCGTTTTCTGATGCTTGGTTTGATGCAATCACTATGGGCTATGGTCTTCGAAATGTTGTTGATAAGCGTAAAGCCATGCAGGAAATTTTACGTGTGCTGAAGCCCGGTAGCTGTTGTATCTCAAGTCAATAACATATTGATTGGTGTTTATATAAAATTTGACACGTGCTACTTTTTACTTTCCAGGTTCTACAGCATCTATCCTTGACTTTAATAAAAGCAATGAGCTACTAACTTCTTCATTTACGGTATACCCTTGCAACATTTATGTTTTGCGCTGCTTAAGTTTAGAGGAAAATCTATTTTTGACATGTGTATATGAATATTAAATTACAATAATAATTTCAATTAACTTGCCTTCGTCTTTTTGATTCTTTGGTGTGAGCGGAAGGTCTAACCCCATTTCTCGTTCTAGGAGTGGATGATTGACAATATTGTTGTCCCTGTAGCCTCTGTTTATGGCCTTTCAGATGACTACAAATATCTCAAAAGTTCAATAAGAGAATTTTTAACAGGTTTGTTTTCAATTGAACTGAAGGCATTTATCCGATCAAGCGTAAATTGGAACATGTTGATTCTGTTTTAACATATTTTTTTTATAGTAGTAAGAGGAAAATACTAAGTGATCATGTTTATCGATGTTAGAACTTTGTCATGTTGATCACCTAACTTAATAATACTCCTGTGATGCAGGGAAGGAATTGGAGAAACTTGCTTTAGAAGTAGGATTTTCTACTGCTCGACATTATGAGATCGGTGGAGGCCTCATGGGGTGCTTGGTAGCTAAACATTAG
Protein sequence

>MS.gene027672.t1

MFPAVAPYPSFRPTSIRCSNDRQALFNRIAPVYDNLNDLLSLGQHRIWKRMAVSWTEAKKGDRVLDVCCGSGDLSFLLSNKVASNGKVIGLDFSKEQLMVASSRSLSKNCFNNIEWVEGDALDLPFSDAWFDAITMGYGLRNVVDKRKAMQEILRVLKPGSTASILDFNKSNELLTSSFTEWMIDNIVVPVASVYGLSDDYKYLKSSIREFLTGKELEKLALEVGFSTARHYEIGGGLMGCLVAKH